Microbial Physiology Commons^™

Biotransformation Of Bile Acids, Cholesterol And Steroids. Chapter 13 In: The Ecology And Physiology Of Gastrointestinal Microbes. Vol. 1, New York, Chapman And Hall., Stephen Baron, Phillip B. Hylemon

Biology Faculty Scholarship

No abstract provided.

Hypercholesterolemia And Platelet Sensitivity To Thromb In-Induced Aggregation In Whole Blood, Charles E. Hammond Ii

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Arts and Sciences at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Sciences in Biology by Charles E. Hammond, II on August 1, 1989.

Protoporphyrinogen Oxidation, A Step In Heme Synthesis In Soybean Root Nodules And Free-Living Rhizobia., Nicholas J. Jacobs, Susan E. Borotz, Mary Lou Guerinot

Dartmouth Scholarship

Extracts of the crude bacteroid fraction of symbiotically grown Bradyrhizobium japonicum were much more active in oxidizing protoporphyrinogen to protoporphyrin than were extracts of cells grown under free-living conditions, especially when assayed in atmospheres containing only traces of oxygen. This correlates with the higher heme content of the microaerophilic nodules. Furthermore, the high level of oxidative activity in the crude bacteroid fraction was associated with an uncharacterized membrane fraction, probably of plant origin, that was separable from the bacteroids by Percoll gradient centrifugation.

Microbial Degradation Of Oxalate In The Gastrointestinal Tracts Of Rats, Steven Daniel

Faculty Research & Creative Activity

Rates of oxalate degradation by mixed bacterial populations in cecal contents from wlld rats ranged from 2.5 to 20.6 µmol/g (dry weight) per h. The oxalate-degrading activity in cecal contents from three strains of laboratory rats (Long-Evans, Wistar, and Sprague-Dawley) from four commercial breeders was generally lower, ranging from 1.8 to 3.5 µmollg (dry weight) of cecal contents per h. This activity did not increase when diets were supplemented with oxalate. Wben Sprague-Pawley rats from a fifth commercial breeder were fed an oxalate diet, rates of oxalate degradation in cecal contents increased from 2.0 to 23.1 µmollg (dry weight) per …

Carbon Monoxide-Dependent Chemolithotrophic Growth Of Clostridium Thermoautotrophicum, M. Savage, Zhongren Wu, Steven Daniel, Leon Lundie, Harold Drake

Faculty Research & Creative Activity

The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO --> CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; Y H2 , was considerably lower than Y CO Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.

Carbon Monoxide-Dependent Chemolithotrophic Growth Of Clostridium Thermoautotrophicum, M. Dean Savage, Zhongren Wu, Steven L. Daniel, Leon L. Lundie Jr, Harold L. Drake

Faculty Research & Creative Activity

The acetogen Clostridium thermoautotrophicum was cultivated under CO-dependent chemolithotrophic conditions. CO-dependent growth profiles and energetics indicated that supplemental CO2 was fundamental to efficient growth at the expense of CO. Overall product stoichiometry approximated 6.5CO --> CH3CO2H + 3.5CO2 + 0.6 cell C + 0.5 unrecovered C. Initial CO/CO2 ratios of 2 to 4 yielded optimal doubling times and cell yields. Maximal YCO values approximated 2.5 g of cell dry weight per mol of CO consumed; Y H2 , was considerably lower than Y CO Cross-transfer growth experiments and protein profiles indicated differential expression of genes between CO and methanol cultures.

Microbial Degradation Of Oxalate In The Gastrointestinal Tracts Of Rats, Steven L. Daniel

Faculty Research & Creative Activity

Rates of oxalate degradation by mixed bacterial populations in cecal contents from wlld rats ranged from 2.5 to 20.6 µmol/g (dry weight) per h. The oxalate-degrading activity in cecal contents from three strains of laboratory rats (Long-Evans, Wistar, and Sprague-Dawley) from four commercial breeders was generally lower, ranging from 1.8 to 3.5 µmollg (dry weight) of cecal contents per h. This activity did not increase when diets were supplemented with oxalate. Wben Sprague-Pawley rats from a fifth commercial breeder were fed an oxalate diet, rates of oxalate degradation in cecal contents increased from 2.0 to 23.1 µmollg (dry weight) per …

Comparison Of Surface Polysaccharides Of R. Leguminosarum Anu57 (Exo-1), R. Leg. Anu57(Pbr1an) (Nod+, Nif-), And R. Leg. Anu57(Pjb5j1) (Nod+, Nif-), Frank G. Hustmyer

Masters Theses

Extracellular polysaccharides (EPS) and lipopolysaccharides (LPS) are suspected to be involved in the legume/Rhizobium symbiotic process. Polysaccharide compositions from the EPS, LPS, capsular polysaccharide (CPS), and small polysaccharide (SmPS) were compared between Rhizobium leguminosarum ANU57 (parent, Exo^-1) and two mutants R. leg. ANU57(pBR1AN) (nod⁺, nif^-) and R. leg. ANU57(pJB5J1) (nod⁺, nif^-) both containing a Sym (symbiotic) plasmid insertion. R. leg. ANU57(pBR1AN) and R. leg. ANU57(pJB5J1) are nod⁺ on clover and peas, respectively. The nod⁺ mutants produce larger amounts of EPS and decreased amounts of LPS compared to …

A Genetic And Physiological Study Of An Arsenite Resistant, Uncoupled Mutant Of Escherichia Coli, Daniel Gordon Smiley

Dissertations and Theses

Chromosomally determined arsenate resistance in Escherichia coli is well characterized. Little, however, is known about chromosomally determined arsenite resistance in E. coli. Accordingly, spontaneous arsenite resistant mutants were selected in a plasmid free strain of E. coli. One mutant strain was analyzed in detail, genetically and physiologically. The mutation confering arsenite resistance was shown to be a single gene mutation. Genetic mapping studies using conjugation and transduction showed that the mutation was closely linked to the ilv region of the E. coli map.

Physiological studies of this mutant showed that it shared characteristics common to those of mutants …

A Taxonomic Study Of Members Of The Family Entrobacteriaceae With Gas Chromatography, Madeline Ducate

Biological Sciences Theses & Dissertations

Abstract unavailable.

Repression Of Β-Galactosidase Synthesis In Escherichia Coli By Salicylates, Joan Carlyn Olson

Dissertations and Theses

Salicylic acid, and to a lesser extent aspirin, have been shown to repress β-galactosidase synthesis in Escherichia coli. The repression is not due to decreased inducer uptake, nor does it result from competition with inducer for repressor. Dinitrophenol does not exert similar effects on β-galactosidase synthesis. Cyclic adenosine 31, 51-monophosphate partially relieves the repression. The extent or the relief by cyclic adenosine monophosphate seems to depend on the concentration of salicylic acid. This indicates there may be direct interaction between the actions of cyclic adenosine monophosphate and salicylic acid in E. coli cells.

The Affect Of Indole-3-Acetic Acid On The Fission Rate Of Paramecium Caudatum, Margherita L. Baker

Student Scholarship

This research report, authored by Margherita L. Baker in 1951, investigates the physiological effects of the plant growth hormone indole-3-acetic acid, also known as heteroauxin, on the fission rate of the protozoan Paramecium caudatum. The study is grounded in the hypothesis that if plant hormones can influence animal growth, it suggests a fundamental similarity in the growth mechanisms between the two kingdoms. The experimental procedure was divided into two distinct series to observe how varying environmental conditions and food availability influenced the hormonal impact. 

In the first series, paramecia were maintained in a nutrient-rich beef extract medium with an abundant …