Microbiology Commons^™

Transport Of E. Coli In Egyptian Agricultural Soils As A Result Of Reclaimed Wastewater Use For Irrigation, Aimen Badawy

Archived Theses and Dissertations

Limited water resources are one of the main constraints to socio-economic development and even a source of insecurity at national and regional levels. The increasing demand for water in the agricultural sector stimulated the use of reclaimed wastewater for irrigation. Comprehending health and environment risks resulting from contaminants that may often be associated with reclaimed wastewater will lead to the sustainable use of this resource, as well as the conservation of other water resources.

In the present study the fate and transport of one important wastewater constituent, namely E. Coli bacteria, in three Egyptian soils of typical agricultural use was …

Effects Of Commercial Diazinon And Imidacloprid On Microbial Urease Activity In Soil And Sod, C. W. Ingram, Mark S. Coyne, David W. Williams

Plant and Soil Sciences Faculty Publications

Diazinon [O,O-diethyl O-2-isopropyl-6-methyl(pyrimidine-4-yl) phosphorothioate] and imidacloprid [1-(1-[6-chloro-3-pyridinyl]methyl)-N-nitro-2-imidazolidinimine] are applied to lawns for insect control simultaneously with nitrogenous fertilizers such as urea, but their potential effect on urease activity and nitrogen availability in turfgrass management has not been evaluated. Urease activity in enzyme assays, washed cell assays, and soil slurries was examined as a function of insecticide concentration. Intact cores from field sites were used to assess the effect of insecticide application on urease activity in creeping bentgrass (Agrostis palustris Huds.) and bluegrass (Poa pratensis L.) sod. Bacterial urease fromBacillus pasteurii and plant …

Fertilizer, Tillage, And Dairy Manure Contributions To Nitrate And Herbicide Leaching, C. S. Stoddard, John H. Grove, Mark S. Coyne, William O. Thom

Plant and Soil Sciences Faculty Publications

Few studies have examined the water quality impact of manure use in no-tillage systems. A lysimeter study in continuous corn (Zea mays L.) was performed on Maury silt loam (fine, mixed, semiactive, mesic Typic Paleudalf) to evaluate the effect(s) of tillage (no-till [NT] and chisel-disk [CD]), nitrogen fertilizer rate (0 and 168 kg N ha⁻¹), and dairy manure application timing (none, spring, fall, or fall plus spring) on NO₃–N, atrazine (2-chloro-4-ethylamino-6-isopropylamino-s-triazine), and alachlor [2-chloro-2′-6′-diethyl-N-(methoxymethyl)acetanilide] concentrations in leachate collected at a 90-cm depth. Herbicides were highest immediately after application, declining to less …

Powdery Mildew In Wine Grapes In Western Australia, Diana Fisher, Trevor Wicks Dr

Bulletins 4000 -

Powdery mildew is caused by the fungal pathogen Uncinula necator. It is the most persistent fungal problem of grapes in WA and one of the most widespread fungal diseases of grapevines in the world. It is characterised by ash-grey to white powdery growth on green tissue of the vine. If uncontrolled it can cause serious crop losses and impair wine quality.

Mortality Of Escherichia Coli O157:H7 In Two Soils With Different Physical And Chemical Properties, D. N. Mubiru, Mark S. Coyne, John H. Grove

Plant and Soil Sciences Faculty Publications

Wild and domesticated animals can harbor a pathogenic Escherichia coli strain designated as O157:H7. Potential health problems could occur if strain O157:H7 is a more robust survivor in defecated waste than commonly used indicator bacteria. A laboratory study was conducted to assess E. coli O157:H7 survival relative to a nonpathogenie E. coli strain in two soils with different physical and chemical characteristics. Bacteria in the inoculated soils were enumerated on a weekly basis for 8 wk using a most probable number (MPN) technique. First-order decay models were used to describe bacteria mortality in the soils. Decay series were described slightly …

Fecal Bacteria Survival And Infiltration Through A Shallow Agricultural Soil: Timing And Tillage Effects, C. S. Stoddard, Mark S. Coyne, John H. Grove

Plant and Soil Sciences Faculty Publications

Human and livestock exposure to fecal pathogens via contaminated surface or groundwater is an important water quality concern for soils receiving animal wastes. The effects of manure application timing (spring or fall application) and soil management (no-tillage or conservation tillage) on fecal bacteria infiltration through shallow karst soils in central Kentucky (the Bluegrass region) have not been evaluated. We performed a field experiment to measure fecal coliforms and fecal streptococci in leachate from dairy manure-amended no-tillage and conservation tillage soils. Manure significantly increased fecal bacteria in leachate compared with unmanured treatments. After manure application, the leachate that collected in zero-tension …

Fecal Coliform Transport Through Intact Soil Blocks Amended With Poultry Manure, S. W. Mcmurry, Mark S. Coyne, E. Perfect

Plant and Soil Sciences Faculty Publications

Poultry production in Kentucky increased almost 200% between 1991 and 1995. Their waste is typically land applied, and fecal pathogen runoff and infiltration may cause nonpoint source groundwater pollution. We looked at the preferential flow of fecal coliforms through undisturbed soil blocks since fecal bacteria typically infiltrate the soil profile to contaminate groundwater. Poultry manure was uniformly distributed on top of sod-covered or tilled (upper 12.5 cm) soil blocks and the blocks were irrigated. Drainage was collected in 100 uniformly spaced cells beneath each block and analyzed for fecal coliform content and drainage volume. The spatial distribution of drainage and …

A Comparative Study Of The Benthic Population In Weir Ponds Draining Watersheds Of The Fernow Experimental Forest, Parsons, West Virginia, Kevin Dwayne Brittingham

Theses, Dissertations and Capstones

A study of the benthos of weir ponds draining watersheds of the Fernow Experimental Forest (FEF) was conducted in 1994-1995. The objectives of this study were: (1) to relate differences in weir pond faunas to watershed treatments on the FEF, and (2) to compare current weir pond faunas to those described in 1973 by Steve Harris. In 1971-1972, there were fewer invertebrates but greater taxa richness than in 1994-1995. Total density varied among weir pond and between studies with numbers in the 1971- 1972 study ranging from 26,168 to 79,259 individuals collected, while numbers in the 1994-1995 study raged from …

Effect Of Sediment Particle Size And Temperature On Fecal Bacteria Mortality Rates And The Fecal Coliform/Fecal Streptococci Ratio, J. M. Howell, Mark S. Coyne, P. L. Cornelius

Plant and Soil Sciences Faculty Publications

Extended survival of fecal bacteria in sediment can obscure the source and extent of fecal contamination in agricultural settings. The variability in fecal coliform/fecal streptococci (FC/FS) ratios with time and discrepancies between observable fecal sources and measured FC/FS ratios in shallow surface water from agricultural watersheds may be explained by examining FC and FS mortality rates in response to ambient temperature and sediment particle size. We measured FC and FS mortality rates at three different temperatures and in three feces-amended sediments with different particle size in a laboratory study. In controlled conditions, using physiological saline to reduce cell death by …

So You Want To Be A Soil Microbiologist?, Mark S. Coyne

Plant and Soil Sciences Faculty Book Gallery

A tongue-in-cheek look at soil microbiology.

Nitrous Oxide Loss From Poultry Manure-Amended Soil After Rain, Mark S. Coyne, A. Villalba, Robert L. Blevins

Plant and Soil Sciences Faculty Publications

Land application of poultry wastes in Kentucky will increase as the broiler industry grows. If poultry manure stimulates N₂O loss from soil it will reduce the fertilizer N value of this waste. In contrast, stimulated N₂O loss in grass filter strips receiving the runoff from manured fields could help reduce contamination of surface water by NO⁻₃. Our objectives were to determine (i) if poultry manure stimulated N₂O loss in soil after rainfall and (ii) if there was an edge-of-field effect on N₂O loss in grass filters intercepting runoff from …

Fecal Bacteria In Agricultural Waters Of The Bluegrass Region Of Kentucky, J. M. Howell, Mark S. Coyne, P. L. Cornelius

Plant and Soil Sciences Faculty Publications

Agricultural runoff influenced by nonpoint pollution frequently exceeds the USEPA standards for bacterial contamination of primary contact water (200 fecal coliforms/100 mL). Few studies have evaluated the effect of cattle (Bos taurus) grazing on fecal contamination of ground water in the karst topography of central Kentucky. Our objectives were to: (i) observe the extent and pattern of fecal bacteria in agricultural waters from two central Kentucky watersheds; (ii) determine if monthly sampling accurately assessed the extent and variability of fecal contamination; and (iii) assess the fecal coliform/fecal streptococci ratio (FC/FS) as an indicator of fecal bacteria source. Springs, …

Lichen Use By Larval Leucochrysa Pavida (Neuroptera:Chrysopidae), Penni Jo Wilson

Masters Theses

Leucochrysa pavida is a species of green lacewing (Neuroptera:Chrysopidae) that uses lichen fragments to build a larval debris "packet." The "packet" is eventually used to form a cocoon used during pupation. Only minute lichen fragments are harvested for formation of the packets which makes identification of the actual lichens utilized difficult using morphological characteristics. For this reason, thin-layer chromatography was employed. The thin-layer chromatographic process identifies acetone-soluble lichen chemicals that are formed in lichen thalli. By comparing the chemistry of lichen communities at Tower Rock Recreation Area in Hardin County, Illinois, with the chemicals identified during thin-layer chromatography of larval …

Nitrous Oxide Flux From Poultry-Manured Erosion Plots And Grass Filters After Simulated Rain, Mark S. Coyne, R. A. Gilfillen, Robert L. Blevins

Plant and Soil Sciences Faculty Publications

Adding carbon-rich materials to fields, like manure, may enhance denitrification. Grass filters, which are used to trap surface runoff from these fields, may also provide a carbon-rich environment that favors water infiltration and denitrification. Nitrous oxide (N₂O) may be evolved these settings. It is a radiatively important trace gas and intermediate in the denitrification pathway and several other microbial processes. We measured N₂O flux, after simulated rain, using a soil cover technique in poultry-manured plots and grass filters receiving their runoff. Intact soil cores were used to relate the N₂O flux to the denitrification …

Virus Diseases Of Subterranean Clover Pastures And Their Management, David Ferris, Roger Jones

Journal of the Department of Agriculture, Western Australia, Series 4

In Western Australia and southern Australia generally, subterranean clover mottle (SCMV) and bean yellow mosaic (BYMV) are the most damaging virus diseases of subterranean clover pastures.

Although infected plants cannot be cured of virus infection, pastures can be managed to reduce both virus spread and persistence from year to year. Management options depend on which virus is present and include reseeding with resistant varieties; changing the pasture composition so that non-host species predominate; altering grazing practices to limit spread; and application of pesticide sprays to kill aphids.

Miscellaneous Publication No 33/93 - Virulent Footrot A Farmer's Guide To Eradication, Department Of Agriculture, Western Australia

Animal production and livestock published reports

The first thing to understand about virulent footrot is that it can occur on any farm in the medium to high rainfall zone. Persons who regularly buy and sell sheep have a greater risk, but even closed breeding flock properties are at risk from stray sheep.

People often associate footrot with properties managed by inefficient farmers. This is a false belief. Many good farmers have found the disease in their sheep, including high profile studs.

Footrot can strike anywhere and often without warning.

The important thing to remember is that once it is found, getting to know and understand the …

Frequency Of Mug Negative Escherichia Coli In Kentucky Groundwater Samples, Mark S. Coyne, J. C. Shuler

Plant and Soil Sciences Faculty Publications

MUG negative Escherichia coli are a small fraction (2.5%) of the total E. coli in Kentucky groundwater samples. It is unlikely that they alone will cause a significant potential to underestimate fecal contamination using MUG as the primary criterion for that assessment. An unresolved question is how effectively MUG-based, defined-substrate tests address false negative water samples containing MUG positive E. coli.

Miscellaneous Publication 39/91 - How To Eradicate Virulent Footrot From Your Property. A Farmer's Guide., Dianne Hopkins

Animal production and livestock published reports

The first thing to understand about virulent footrot is that it can occur on any farm in the medium to high rainfall zone.

People often associate footrot with properties managed by inefficient farmers. This is a belief based on fallacy. Many good farmers have found the disease in their sheep, including high profile stud producers.

Footrot can strike anywhere and without warning.

The important thing to remember is, that once it is found, getting to know the disease and understanding it will help you beat it. This manual, the video and your stock inspector will help you eradicate footrot from …

Plant Viruses., R. A.C. Jones

Experimental Summaries - Plant Research

Cucumber mosaic virus in legumes, 85A024, 85A122, 85A123, 85A153, 85A163, 85A217, 85A219. Alfalfa mosaic virus in annual medics.

Rhizoctonia Patch Of Cereals, G C. Macnish

Journal of the Department of Agriculture, Western Australia, Series 4

Rhizoctonia patch is now widespread within Western Australia, having been recorded on all cereals and on a wide variety of other crops and pasture plants. Although the disease-as its name implies - looks spectacular in the field, the overall reduction in crop yield is probably small. However, its increasing frequency is causing considerable concern as the disease appears to be associated with minimum or reduced tillage systems.

The Effect Of A Bacteriophage On The Competition Of Rhizobium Japonicum Strains For Nodulation Of Glycine Max (L.) Merr, Lori Kelley

Masters Theses

The symbiotic relationship between Rhizobium japonicum (Kir.) Buch. and Glycine max (L.) Merr. was the concern of this thesis. This relationship occurs naturally in soybean fields. In the field there are many different strains of Rhizobium. Some of these strains fix atmospheric nitrogen more efficiently than others resulting in a higher crop yield. The problem is the more efficient nitrogen fixers are out-competed by less efficient nitrogen fixers. In order to solve this problem the more competitive strains have to be controlled. There are two main ways to do this. One is to increase the number of the desired …

Partial Characterization Of The Extracellular Polysaccharides Produced By Mutants Of Rhizobium Leguminosarum And Rhizobium Trifolii, Lendell L. Cummins

Masters Theses

Extracellular polysaccharides (EPS) are suspected to be involved in some aspect of the Rhizobium-legume symbiotic process. Extracellular polysaccharides produced by R. leguminosarum and R. trifolii mutants were isolated and partially characterized by gas chromatography, column chromatography, double diffusion, polyacrylamide gel electrophoresis, and immunoelectrophoresis.

The R. leguminosarum mutants, derived from strain 128C53, all are deficient in EPS production (exo-). Strain ANU54 is missing the symbiotic plasmid, while plasmid-containing strains ANU54 pJB5JI and ANU54 pBR1AN nodulate (nod+) peas and clover respectively, but do not fix nitrogen (fix-).

R. leguminosarum mutants produce an EPS that is similar to parental LPS in composition. …

Benthic Diatom Community Dynamics In The Colorado River: Interactive Effects Of Periodic Dessication And Current Regime, Christopher Gerard Peterson

Publications (WR)

The response to periodic desiccation of periphyton communities developed in eddy environments or in areas exposed to variable, direct current was examined. Algal communities were incubated on clay tiles for between 2 and 12 weeks in the Colorado River, directly below Hoover Dam from October, 1983 - January, 1984. Mean daily discharge from the dam decreased over this period. To assess the influence of these discharge changes on community dynamics, samples collected over the first 12 weeks of the investigation were compared to samples of the same age collected over the final 8 weeks.

The small diatom taxon Achnahthes spp …

The Biology Of Coprinus Radians, Ozonium, Oidium, And Their Interrelationships, Thomas J. Fahey

All-Inclusive List of Electronic Theses and Dissertations

The pleomorphism of Coprinus radians was studied. The three distinct growth forms--sporophore, oidial stage, and ozonium stage--were examined in relation to ultrastructure, morphology and development. Scanning and transmission electron microscopy are correlated with photomicrographs of developmental stages. Development of the oidia and of the sporophore are of particular interest. The multinucleate oidia develop quite differently from oidia as generally described for Basidiomycete imperfect stages. Development of the oidia of this Coprinus is holoblastic and sympodial. The mushroom primordia are differentiated internally into gill, cap, and stipe hyphae while only l-2mm in diameter. At this stage they look superficially like sclerotial …

Report On Wastewater Disposal To Board Of County Commissioners, Clark County Nevada, Clair N. Sawyer

Publications (WR)

This report is concerned with the management of waste-waters and subsurface drainage from the City of Las Vegas and its environs and the BMI complex, all in Clark County, Nevada. The prime objective is to recommend a plan of action which will utilize the natural resources of the area in the least costly manner and still protect Lake Mead for recreational purposes and use as a public water supply, At this writing, conditions in the upper Las Vegas arm of Boulder Basin are quite unsatisfactory for some recreational purposes due to the extensive blooms of algae which develop. Experience at …

Fixation Of Dormant Tilletia Teliospores For Thin Sectioning, John Scott Gardner

Theses and Dissertations

Dormant Tilletia caries teliospores in fixative solution or distilled water were frozen onto specimen chucks of an FTS Sorvall- Christensen frozen thin sectioner and cut or fractured at various temperatures (-20 to -75 C) and thickness settings (10, 15, 20, and 25 um). Cytoplasm of dormant spores was well preserved and organelles were found to differ from those of germinated spores in morphology. Irregular electron dense and electron opaque areas of lipid bodies were sometimes evident in dormant and germinating Tilletia caries teliospores. When dormant teliospores were sectioned in buffered fixative, lipids had an appearance similar to lipids in thin …

Root Rot Of Subterranean Clover In W.A, G C. Macnish, M. J. Barbetti, D. Gillespie, K. Hawley

Journal of the Department of Agriculture, Western Australia, Series 4

Root rot of subterranean clover has occurred sporadically in the south west of Western Australia for a number of years.

In most seasons the disease has affected the clover paddocks of only a few farms, but in 1973 there was widespread pasture decline due to root rot in the South-West and south coastal districts.

At present the most promising approaches for minimising the effect of root rot appear to be the use of cultivation techniques and eventually the use of resistant varieties, or other pasture species.

The Effect Of Urea On The Sedimentation Coefficient Of The Curly Top Virus Dimer, Allen H. Smith

Undergraduate Honors Capstone Projects

The purpose of this project is to investigate the effect of increasing concentrations of urea on the sedimentation rate of the curly top virus dimer as measured by ultracentrifugation techniques. In general, urea causes the breaking of hydrogen bonds in macromolecules--in the case of the virus, increased concentrations of urea should cause changes in the configuration of the virus and may possibly cause a separation of the dimer into monomers. From previous centrifugation studies, we know that the dimer has a characteristic sedimentation coefficient(S) of about 80 Svedbergs; the monomer has an S value of about 55 Svedberg units. Increased …

Phomopsis : The Causal Fungus Of Lupinosis, P Mcr Wood, A. G. P. Brown

Journal of the Department of Agriculture, Western Australia, Series 4

It is now well known that lupinosis of sheep and cattle follows ingestion of lupin stubble colonised by the fungus Phomopsis rossiana. The fungus infects green lupins but persists on the stubble and under certain conditions apparently produces a toxin which causes the disease.

As Phomopsis is now widespread on lupins in Western Australia its ecology has been studied in detail as a basis for possible control measures.

This article outlines what is known so far of the disease and its control.

Lupinosis, M R. Gardiner

Journal of the Department of Agriculture, Western Australia, Series 4

In spite of many years of intensive investigation lupinosis remains a remarkably complex and baffling problem. Current research is throwing new light on the disease and it is now much better understood— although far from beaten.

Lupinosis research is co-ordinated by the Department of Agriculture's Lupinosis Committee, on which are represented all groups working on the disease. These include veterinary surgeons and pathologists, chemists, plant pathologists and animal husbandry specialists.

Chairman of the Committee is Dr M. R. Gardiner, Chief of the Department's Animal Division and a veteran of lupinosis research.

In this article Dr Gardiner summarises the current state …