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Full-Text Articles in Microbiology
The Quest To Identify A New Virus Disease Of Sunflower From Nebraska, Robert M. Harveson, Maher Al Rwahnih, California Department Of Food And Agriculture, Alex Karasev, Tom J. Gulya, Jeff Bradshaw
The Quest To Identify A New Virus Disease Of Sunflower From Nebraska, Robert M. Harveson, Maher Al Rwahnih, California Department Of Food And Agriculture, Alex Karasev, Tom J. Gulya, Jeff Bradshaw
Panhandle Research and Extension Center
Between 2010 and 2018, sunflower plants exhibiting virus-like symptoms, including stunting, mottling, and chlorotic ringspots on leaves, were observed from commercial fields and research plots from four sites within three distinct counties of western Nebraska (Box Butte, Kimball, and Scotts Bluff). Near identical symptoms from field samples were reproduced on seedlings mechanically in the greenhouse on multiple occasions, confirming the presence of a sap-transmissible virus from each site. Symptomatic greenhouse-inoculated plants from the 2010 and 2011 Box Butte samples tested negative for sunflower mosaic virus (SuMV), sunflower chlorotic mottle virus (SuCMoV), and all potyviruses in general by ELISA and RT-PCR. …
Assembly-Hub Function Of Er-Localized Snare Proteins In Biogenesis Of Tombusvirus Replication Compartment, Zsuzsanna Sasvari, Nikolay Kovalev, Paulina Alatriste Gonzalez, Kai Xu, Peter D. Nagy
Assembly-Hub Function Of Er-Localized Snare Proteins In Biogenesis Of Tombusvirus Replication Compartment, Zsuzsanna Sasvari, Nikolay Kovalev, Paulina Alatriste Gonzalez, Kai Xu, Peter D. Nagy
Plant Pathology Faculty Publications
Positive-strand RNA viruses assemble numerous membrane-bound viral replicase complexes within large replication compartments to support their replication in infected cells. Yet the detailed mechanism of how given subcellular compartments are subverted by viruses is incompletely understood. Although, Tomato bushy stunt virus (TBSV) uses peroxisomal membranes for replication, in this paper, we show evidence that the ER-resident SNARE (soluble NSF attachment protein receptor) proteins play critical roles in the formation of active replicase complexes in yeast model host and in plants. Depletion of the syntaxin 18-like Ufe1 and Use1, which are components of the ER SNARE complex in the ERAS (ER …
Sterol Binding By The Tombusviral Replication Proteins Is Essential For Replication In Yeast And Plants, Kai Xu, Peter D. Nagy
Sterol Binding By The Tombusviral Replication Proteins Is Essential For Replication In Yeast And Plants, Kai Xu, Peter D. Nagy
Plant Pathology Faculty Publications
Membranous structures derived from various organelles are important for replication of plus-stranded RNA viruses. Although the important roles of co-opted host proteins in RNA virus replication have been appreciated for a decade, the equally important functions of cellular lipids in virus replication have been gaining full attention only recently. Previous work with Tomato bushy stunt tombusvirus (TBSV) in model host yeast has revealed essential roles for phosphatidylethanolamine and sterols in viral replication. To further our understanding of the role of sterols in tombusvirus replication, in this work we showed that the TBSV p33 and p92 replication proteins could bind to …
Role Of Viral Rna And Co-Opted Cellular Escrt-I And Escrt-Iii Factors In Formation Of Tombusvirus Spherules Harboring The Tombusvirus Replicase, Nikolay Kovalev, Isabel Fernández De Castro Martín, Judit Pogany, Daniel Barajas, Kunj Bihari Pathak, Cristina Risco, Peter D. Nagy
Role Of Viral Rna And Co-Opted Cellular Escrt-I And Escrt-Iii Factors In Formation Of Tombusvirus Spherules Harboring The Tombusvirus Replicase, Nikolay Kovalev, Isabel Fernández De Castro Martín, Judit Pogany, Daniel Barajas, Kunj Bihari Pathak, Cristina Risco, Peter D. Nagy
Plant Pathology Faculty Publications
Plus-stranded RNA viruses induce membrane deformations in infected cells in order to build viral replication complexes (VRCs). Tomato bushy stunt virus (TBSV) co-opts cellular ESCRT (endosomal sorting complexes required for transport) proteins to induce the formation of vesicle (spherule)-like structures in the peroxisomal membrane with tight openings toward the cytosol. In this study, using a yeast (Saccharomyces cerevisiae) vps23Δ bro1Δ double-deletion mutant, we showed that the Vps23p ESCRT-I protein (Tsg101 in mammals) and Bro1p (ALIX) ESCRT-associated protein, both of which bind to the viral p33 replication protein, play partially complementary roles in TBSV replication in cells …