Genetics and Genomics Commons^™

Cancer Genetic Susceptibility Testing: Ethical And Policy Implications For Future Research And Clinical Practice, Benjamin S. Wilfond, Karen H. Rothenberg, Elizabeth J. Thomson, Caryn Lerman

Faculty Scholarship

No abstract provided.

Sex Chromosome Ratios And Aneuploidy Rates In The Zona Pellucida Selected Spermatozoa From Normal And Subfertile Men, Qinuo Fan Van Dyk

Theses and Dissertations in Biomedical Sciences

The human hemizona has been demonstrated to select spermatozoa with good motility, normal morphology, and the capacity to undergo the zona-induced acrosomal reaction. The studies conducted here are directed at using the human hemizona to investigate two key questions: (1) whether human X-chromosome bearing spermatozoa (X-sperm) and Y-chromosome bearing spermatozoa (Y-sperm) differ in their functional survival time (assessed by their capacity to bind to the human zona pellucida) after prolonged in vitro incubation, and (2) whether hemizona-bound spermatozoa have a reduced aneuploidy rate compared to unbound (and therefore, unselected) spermatozoa?

In the functional survival study, donor spermatozoa were held in …

Draft: Optimising The Worth Of The Lobster Catch: Options And Issues, Marec Pty Ltd

Fisheries Management Papers

The purpose of this document is to contribute to informed discussion on alternative measures which might be adopted to optimise the worth of the lobster catch. In doing so, it provides a number of possible initiatives which could be more closely examined for adoption. Details of issues are provided which should be taken into consideration when assessing the various proposals. The industry as it is today is examined and assessed, and its achievements analysed. The possible initiatives proposed stem from this research. As a consequence of this document, the rock lobster industry could undertake to proceed with a detailed examination …

Selective Feeding By Phyllostomid Fruit Bats In A Subtropical Montane Cloud Forest, David Herníndez-Conrique, Luis Iñiguez-Dávalos, Jay F. Storz

Jay F. Storz Publications

Phyllostomid bats eat the fruits and disperse the seeds of a wide variety of neotropical plant species, particularly those typical of early successional stages (Fleming & Heithaus 1981, Heithaus 1982, Orozco-Segovia et al. 1985). In assessing the effects of frugivorous animals on plant populations and vice-versa, it is important to determine whether the animals are selective in the types of fruit they eat, or whether the proportional abundance of different fruit species in their diets simply reflects availability in the environment. The purpose of this study was to test experimentally the hypothesis that two common phyllostomid species, Sturnira ludovici and …

Detection Of Hybridization Events Between The Coyote, Canis Latrans, And The Domestic Dog, Canis Familiaris, Using Two Polymorphic Microsatellite Loci And Cranial Morphometric Analysis, John Jobe Cox

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by John Jobe Cox on August 26, 1997.

Dna Fingerprinting Reveals Low Genetic Diversity In Gunnison's Prairie Dog (Cynomys Gunnisoni), Steven E. Travis, C. N. Slobodchikoff, Paul Keim

Genetics Collection

The use of molecular techniques for the assessment of familial relationships among social species of mammals has become relatively commonplace. However, some species represent poor candidates for such studies due to naturally low levels of genetic diversity, leading to unacceptably large standard errors associated with estimates of relatedness. Here, we report on a preliminary study of genetic diversity within two populations of a social species of ground squirrel, Gunnison's prairie dog (Cynomys gunnisoni) using DNA fingerprinting. We observed low levels of diversity in the form of large mean coefficients of genetic similarity among individuals occupying the same population. …

Identification Of A Gal/Galnac Lectin In The Protozoan Hartmannella Vermiformis As A Potential Receptor For Attachment And Invasion By The Legionnaires' Disease Bacterium, Chandrasekar Venkataraman, Bradley J. Haack, Subbarao Bondada, Yousef Abu Kwaik

Microbiology, Immunology, and Molecular Genetics Faculty Publications

The Legionnaire's disease bacterium, Legionella pneumophila, is a facultative intracellular pathogen which invades and replicates within two evolutionarily distant hosts, free-living protozoa and mammalian cells. Invasion and intracellular replication within protozoa are thought to be major factors in the transmission of Legionnaire's disease. Although attachment and invasion of human macrophages by L. pneumophila is mediated in part by the complement receptors CR1 and CR3, the protozoan receptor involved in bacterial attachment and invasion has not been identified. To define the molecular events involved in invasion of protozoa by L. pneumophila, we examined the role of protein tyrosine phosphorylation …

Marine Farm Planning And Consultation Processes In Western Australia, Dave Everall

Fisheries Management Papers

The development of a sustainable aquaculture industry in Western Australia is considered to be a high priority by the Government. The marine farming industry has a bright future in Western Australia, but there is a need to balance its developing needs for suitable waters with those of existing activities, and with community expectations for accountability and transparency in the resolution of equity and resource sharing decisions. The purpose of this report is to review the existing planning and consultation processes for pearling and aquaculture in WA and recommend how they may be improved and incorporated into a single process. It …

Ms2 Coat Protein Mutants Which Bind Qβ Rna, Marc Spingola, David Peabody

Biology Department Faculty Works

The coat proteins of the RNA phages MS2 and Qβ are structurally homologous, yet they specifically bind different RNA structures. In an effort to identify the basis of RNA binding specificity we sought to isolate mutants that convert MS2 coat protein to the RNA binding specificity of Qβ. A library of mutations was created which selectively substitutes amino acids within the RNA binding site. Genetic selection for the ability to repress translation from the Qβ translational operator led to the isolation of several MS2 mutants that acquired binding activity for Qβ RNA. Some of these also had reduced abilities to …

A. F. J. K. Mayer: Anatomy Of The Eye Of The Cetaceans. An Annotated Translation., Heiko L. Shoenfuss

Occasional Papers of the Museum of Natural Science, Louisiana State University

No abstract provided.

Variation In The Mexican Garter Snake Thamnophis Scalaris Cope And The Taxonomic Status Of T. Scaliger (Jan), Douglas A. Rossman, Guillermo Lara Gongora

Occasional Papers of the Museum of Natural Science, Louisiana State University

No abstract provided.

Percina Kathae, A New Logperch Endemic To The Mobile Basin In Mississippi, Alabama, Georgia, And Tennessee (Percidae, Etheostomatini), Bruce A. Thompson

Occasional Papers of the Museum of Natural Science, Louisiana State University

No abstract provided.

Fluorescence In Situ Hybridization Analysis Of Human Embryos Derived From In Vitro And In Vivo Matured Oocytes, Constance Descisciolo

Theses and Dissertations in Biomedical Sciences

Despite adequate hormonal stimulation, oocytes collected for the purpose of in vitro fertilization and embryo transfer display several levels of nuclear maturity. Preovulatory or mature oocytes, technically those that are Metaphase I or II, are inseminated shortly after aspiration and assessed for fertilization the following day. Prophase I oocytes, also called germinal vesicle-bearing or immature oocytes, require a 24-36 hour period in culture before being exposed to spermatozoa. During this time, the majority of Prophase I oocytes complete nuclear maturation in vitro, progressing from germinal vesicle breakdown through first polar body extrusion. If inseminated, many in vitro matured oocytes fertilize …

Oral Cancer Screening, Damon Omar Watson

Honors Theses

Cancers of the oral cavity and pharynx have increased throughout the decades. Since oral cancer represents a small percentage of the total cancers in the United States, it is often overlooked. Though this is the case, the results of this cancer can be devastating. To address this issue, an Oral Cancer Screening was organized on August 14, 1996 at the West Michigan Cancer Center in Kalamazoo. This was a joint collaboration between the author, the West Michigan Cancer Center, and the Kalamazoo Valley Dental Society. This event allowed research to take place concerning the data obtained. Confidential results and information …

The Aquaculture Of Non-Endemic Species In Western Australia, Tina Thorne

Fisheries Management Papers

This Management Paper has been drafted in liaison with the Department of Environmental Protection in accordance with the principles of risk assessment and taking into account translocation issues of non-endemic species such as redclaw (Cheras quadricarninatus). It is considered that the implementation of this policy will protect the natural environment and native crayfish species. It will also allow for the development of a redclaw industry, and provide the first step towards self regulation of that industry.

Relationships Between In Situ Protein Degradability And Grass Developmental Morphology, R. B. Mitchell, D. D. Redfearn, Lowell E. Moser, R. J. Grant, K. J. Moore, B. H. Kirch

Department of Animal Science: Faculty Publications

The objective of this research was to determine the relationships between the morphological development and in situ ruminally degradable protein (RDP), ruminally undegradable protein (RUP), and microbial protein of two cool season grasses (intermediate wheatgrass and smooth bromegrass) and two warm season grasses (switchgrass and big bluestem). The initial growth of grass tillers grown near Mead, Nebraska was clipped at ground level six times during the 1992 growing season and morphologically classified. Mean stage was calculated. Forage was ground to pass a 2-mm screen and was incubated in ruminally fistulated steers for 16 h. The RUP was adjusted for microbial …

Study Of The Hiv-1 Gene Regulatory Proteins Tat And Rev By The Two-Hybrid System In Yeast, David Allen Elkins

Loma Linda University Electronic Theses, Dissertations & Projects

Tat and Rev, small proteins encoded by the Human Immunodeficiency Virus type 1 (HIV-1), regulate its pattern of gene expression within infected cells. Tat increases transcription from the integrated pro virus by approximately a thousand-fold; Rev effects a shift in the pattern of splicing of the viral mRNA. Both are likely to interact with cellular proteins in executing their respective functions. Tat has additional activities (including neurotoxicity and inhibition of lymphocyte activation) which may be extraneous to its viral functions.

In order to identify cellular proteins interacting with Tat and Rev, the genes for both were cloned into plasmid vectors …

Psychosocial Risks Of Storing And Using Human Tissues In Research, Jon F. Merz

RISK: Health, Safety & Environment (1990-2002)

Dr. Merz argues that genetics technology makes it more compelling that researchers plan more carefully for the collection and disposition of information derived from subjects' tissues and blood.

Coming To Terms With Heritability, Scott F. Stoltenberg

Department of Psychology: Faculty Publications

The complex mechanisms of heredity are little appreciated by nonspecialists, in some measure, because of misunderstandings that are perpetuated when words used for technical terms have other, more widely understood, folk meanings. When a word has both technical and folk meanings, it is the responsibility of the specialist to avoid promoting confusion by either using extremely cautious and precise language when using the term or, in cases when confusion is inevitable, abandoning the term in favor of one without a widely understood folk meaning. The study of heredity is beset by such confusion, and the term heritability appears to be …

Genomic Diversity In Staphylococcus Aureus, Bret Max Barton

Loma Linda University Electronic Theses, Dissertations & Projects

Staphylococcus aureus chromosomal DNA from 125 methicillin-resistant and 10 methicillin-sensitive clinical isolates was digested with rare-cutting restriction endonucleases and subjected to pulsed-field gel electrophoresis (PFGE). Thirty-nine distinct genomic macrorestriction patterns (GPs) were identified using SmaI; 29 of these patterns are from methicillin-resistant S. aureus (MRSA).

A dendrogram showing percent similarity among the patterns was constructed which revealed the considerable genomic diversity of the collection even though the isolates had been obtained during outbreaks. The majority of the MRSA formed one broad group with two outbreak subclasses. However, 8 of the 29 MRSA GPs (28%) were diverse.

Digestion with Csp …

Management Arrangements For The Western Rock Lobster Fishery For The 1997/98 Season, F. B. Prokop

Fisheries Management Papers

This paper deals with the proposed management strategies for the 1997/98 seasons. The purpose of this paper is to present the views of RLIAC on management arrangements for the 1997/98 rock lobster season and to indicate the strategic directions that long term management of this important fishery might take so as to ensure both its biological sustainability and the optimisation of product value for both the State and the participants in the fishery.

Homology Among The Autosomal Chromosomes Of Boophilus Annulatus (Say) And B. Microplus (Canestrini), Bonnie S. Gunn

Theses and Dissertations - UTB/UTPA

Autosomes of Boophilus annulatus and B. microplus were compared using C- and G-bands to establish the degree of homology. Karyotypes of both species consisted of 20 autosomes and an XX:XO sex determination system with the X being the largest chromosome. All chromosomes of B. annulatus were acrocentric with heterochromatin limited to centromeric regions. The B. microplus karyotype was acrocentric with one band of noncentromeric heterochromatin occurring in three chromosome pairs. Interspecific comparisons indicated seven pairs of G-band homologous autosomes and three G-band homologous pairs when added interstitial heterochromatin was considered. The number three chromosome of both species showed variation in …

Percina Suttkusi, A New Species Of Logperch (Subgenus Percina) From Louisiana, Mississippi, And Alabama (Perciformes, Percidae, Etheostomatini), Bruce A. Thompson

Occasional Papers of the Museum of Natural Science, Louisiana State University

No abstract provided.

Y-Chromosome Effects On Drosophila Geotaxis Interact With Genetic Or Cytoplasmic Background, Scott F. Stoltenberg, Jerry Hirsch

Department of Psychology: Faculty Publications

Previously, all of the major fruit fly, Drosophila melanogaster, chromosomes (I, II, and III) have been shown to be associated with geotaxis, but the Y chromosome has not. Using two methods (back-crossing and chromosome substitution), Y chromosomes from lines that have evolved stable, extreme expressions of geotaxis were placed into different genetic and cytoplasmic backgrounds to test the resulting males for geotaxis. The results of the back-crossing do not support the interpretation of Y-chromosome effects on geotaxis. These tests do not have sufficient statistical power, however, to detect small genetic effects. In the chromosome substitution experiment, the geotaxis-line Y …

Genetic Information And The Workplace: Legislative Approaches And Policy Challenges, Karen H. Rothenberg, Barbara Fuller, Mark Rothstein, Troy Duster, Mary Jo Ellis Kahn, Rita Cunningham, Beth Fine, Kathy Hudson, Mary-Claire King, Patricia Murphy, Gary Swergold, Francis Collins

Faculty Scholarship

No abstract provided.

Management Of The South Coast Purse Seine Fishery, J Fowler, K Donohue, R.C. J. Lenanton, M. Moran, D Gaughan

Fisheries Management Papers

This paper has presented a strategy for moving the management of the small pelagics fishery off the south coast to management of the whole fishery, which facilitates a more biologically sound and economically stable fishery.

A Pricing Policy For Fisheries Agencies, Management Committee Of Scfa, Standing Committee Of Fisheries And Aquaculture (Sfca), Department Of Primary Industries And Energy

Fisheries Management Papers

The intent of this paper is to inform members of Standing Committee for Fisheries and Aquaculture (SCFA) and the members of the Ministerial Council for Fisheries, Forestry and Aquaculture of the methods of fee setting that are currently applied by fisheries agencies in Australia, and alternative pricing policies for fisheries agencies.

Identification Of Poinsettia Cultivars Using Rapd Markers, Jing-Tian Ling, Roger Sauve, Nick Gawel

Agricultural and Environmental Sciences Faculty Research

Randomly amplified polymorphic DNA (RAPD) techniques were used to compare the DNA from leaf tissues of nine commercial poinsettia (Euphorbia pulcherrima Wild ex Klotzsch) cultivars. Amplification occurred in 57 out of 60 (95%) tested primers. Nine primers that revealed polymorphisms among cultivars were selected for further evaluation. Forty-eight RAPD bands were scored from these primers, and 33 (69%) were polymorphic. All tested cultivars could be discriminated with seven bands generated from primers OPB7 and OPC13. Results of a UPGMA cluster analysis and principal components analysis placed the nine cultivars into two groups: one group consisted of `Jingle Bells', `Supjibi', …

Selection Of Populations Of Puccinia Recondita F. Sp. Tritici For Shortened Latent Period On A Partially Resistant Wheat Cultivar, Jeffery S. Lehman, Gregory Shaner

Biology and Earth Science Faculty Scholarship

Wild-type fungal population 851-WT was selected for shortened latent period on cv. CI 13227 for five uredinial generations to study the adaptation of Puccinia recondita f. sp. tritici to partially resistant wheat cultivars. Differences among wild-type and selected populations for traits contributing to parasitic fitness (i.e., latent period, infection frequency, and uredinium area and growth rate) were assessed in monocyclic infection experiments on susceptible cv. Monon and partially resistant cvs. Suwon 85, Sw 72469-6, L-574-1, and CI 13227. Differences were greatest among fungal populations on cv. CI 13227. The mean latent period of selected population 851-C5 was 2 days shorter …

The Analysis Of Twelve Forensic Dna Genetic Markers For Hardy-Weinberg And Gametic Phase Disequilibrium For A Caucasian Data Base, Daniel John Gregonis

Theses Digitization Project

No abstract provided.