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- Proteomics (13)
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- Fruit quality (5)
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- Ethylene (3)
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- Jackfruit (3)
- Lupinus angustifolius (3)
- Malting (3)
- Melatonin (3)
- Methyl jasmonate (3)
- Microalgae (3)
- SWATH-MS (3)
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Articles 31 - 60 of 89
Full-Text Articles in Food Science
Simultaneous Extraction And Quantitative Analysis Of S-Methyl-L-Cysteine Sulfoxide, Sulforaphane And Glucosinolates In Cruciferous Vegetables By Liquid Chromatography Mass Spectrometry, Armaghan Shafaei, Caroline R. Hill, Jonathan M. Hodgson, Lauren C. Blekkenhorst, Mary C. Boyce
Simultaneous Extraction And Quantitative Analysis Of S-Methyl-L-Cysteine Sulfoxide, Sulforaphane And Glucosinolates In Cruciferous Vegetables By Liquid Chromatography Mass Spectrometry, Armaghan Shafaei, Caroline R. Hill, Jonathan M. Hodgson, Lauren C. Blekkenhorst, Mary C. Boyce
Research outputs 2022 to 2026
Sulfur containing compounds including glucosinolates (GLS), sulforaphane (SFN) and S-methyl-L-cysteine sulfoxide (SMCSO) have been proposed to be partly responsible for the beneficial health effects of cruciferous vegetables. As such, greater understanding of their measurements within foods is important to estimate intake in humans and to inform dietary intervention studies. Herein is described a simple and sensitive method for simultaneous analysis of 20 GLS, SFN and SMCSO by liquid chromatography mass spectrometry. Analytes were effectively retained and resolved on an Xbridge C18 column. Detection can be achieved using high resolution or unit resolution mass spectrometry; the latter making the method more …
Increased Nitrate Intake From Beetroot Juice Over 4 Weeks Affects Nitrate Metabolism, But Not Vascular Function Or Blood Pressure In Older Adults With Hypertension, Rebeka Fejes, Martin Lutnik, Stefan Weisshaar, Nina Pilat, Karl-Heinz Wagner, Hans-Peter Stüger, Jonathan M. Peake, Richard J. Woodman, Kevin D. Croft, Catherine P. Bondonno, Jonathan M. Hodgson, Michael Wolzt, Oliver Neubauer
Increased Nitrate Intake From Beetroot Juice Over 4 Weeks Affects Nitrate Metabolism, But Not Vascular Function Or Blood Pressure In Older Adults With Hypertension, Rebeka Fejes, Martin Lutnik, Stefan Weisshaar, Nina Pilat, Karl-Heinz Wagner, Hans-Peter Stüger, Jonathan M. Peake, Richard J. Woodman, Kevin D. Croft, Catherine P. Bondonno, Jonathan M. Hodgson, Michael Wolzt, Oliver Neubauer
Research outputs 2022 to 2026
The decline in vascular function and increase in blood pressure with aging contribute to an increased cardiovascular disease risk. In this randomized placebo-controlled crossover study, we evaluated whether previously reported cardiovascular benefits of plant-derived inorganic nitrate via nitric oxide (NO) translate into improved vascular function and blood pressure-lowering in 15 men and women (age range: 56-71 years) with treated hypertension. We investigated the effects of a single ∼400 mg-dose at 3 hours post-ingestion (3H POST) and the daily consumption of 2 × ∼400 mg of nitrate through nitrate-rich compared with nitrate-depleted (placebo) beetroot juice over 4 weeks (4WK POST). Measurements …
Effect Of High-Pressure Processing On The Antioxidant And Antibacterial Properties Of Honey, Ayesha Faraz, Mark Williams, Wmad Binosha Fernando, Vijay Jayasena
Effect Of High-Pressure Processing On The Antioxidant And Antibacterial Properties Of Honey, Ayesha Faraz, Mark Williams, Wmad Binosha Fernando, Vijay Jayasena
Research outputs 2022 to 2026
High pressure processing (HPP) have the potential to be used as an alternative to commercial thermal processing of honey. Its impact on the antioxidant and antibacterial properties of honey have yet to be understood properly. This study focuses on the effects of HPP on the antioxidant and antibacterial properties of honey. Six different honey samples were subjected to HPP at 400 and 600 MPa for 10 and 15 min. The antioxidant properties were measured in terms of total phenolic content, total flavonoid content, the 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assay, and 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) free radical scavenging assay. The antibacterial activity …
Rapid Discrimination Between Wild And Cultivated Ophiocordyceps Sinensis Through Comparative Analysis Of Label-Free Sers Technique And Mass Spectrometry, Qing Hua Liu, Jia Wei Tang, Zhang Wen Ma, Yong Xuan Hong, Quan Yuan, Jie Chen, Xin Ru Wen, Yu Rong Tang, Liang Wang
Rapid Discrimination Between Wild And Cultivated Ophiocordyceps Sinensis Through Comparative Analysis Of Label-Free Sers Technique And Mass Spectrometry, Qing Hua Liu, Jia Wei Tang, Zhang Wen Ma, Yong Xuan Hong, Quan Yuan, Jie Chen, Xin Ru Wen, Yu Rong Tang, Liang Wang
Research outputs 2022 to 2026
Ophiocordyceps sinensis is a genus of ascomycete fungi that has been widely used as a valuable tonic or medicine. However, due to over-exploitation and the destruction of natural ecosystems, the shortage of wild O. sinensis resources has led to an increase in artificially cultivated O. sinensis. To rapidly and accurately identify the molecular differences between cultivated and wild O. sinensis, this study employs surface-enhanced Raman spectroscopy (SERS) combined with machine learning algorithms to distinguish the two O. sinensis categories. Specifically, we collected SERS spectra for wild and cultivated O. sinensis and validated the metabolic profiles of SERS spectra using Ultra-Performance …
Advancing Sustainable Malting Practices: Aquaporins As Potential Breeding Targets For Improved Water Uptake During Controlled Germination Of Barley (Hordeum Vulgare L.), Clare E. O’Lone, Angéla Juhász, Mitchell Nye-Wood, David Moody, Hugh Dunn, Jean P. Ral, Michelle L. Colgrave
Advancing Sustainable Malting Practices: Aquaporins As Potential Breeding Targets For Improved Water Uptake During Controlled Germination Of Barley (Hordeum Vulgare L.), Clare E. O’Lone, Angéla Juhász, Mitchell Nye-Wood, David Moody, Hugh Dunn, Jean P. Ral, Michelle L. Colgrave
Research outputs 2022 to 2026
The conversion of raw barley (Hordeum vulgare L.) to malt requires a process of controlled germination, where the grain is submerged in water to raise the moisture content to > 40%. The transmembrane proteins, aquaporins, influence water uptake during the initial stage of controlled germination, yet little is known of their involvement in malting. With the current focus on sustainability, understanding the mechanisms of water uptake and usage during the initial stages of malting has become vital in improving efficient malting practices. In this study, we used quantitative proteomics analysis of two malting barley genotypes demonstrating differing water-uptake phenotypes in the …
Insights Into Phytonutrient Profile And Postharvest Quality Management Of Jackfruit: A Review, Jashanpreet Kaur, Zora Singh, Hafiz Muhammad Shoaib Shah, Muhammad Sohail Mazhar, Mahmood Ul Hasan, Andrew Woodward
Insights Into Phytonutrient Profile And Postharvest Quality Management Of Jackfruit: A Review, Jashanpreet Kaur, Zora Singh, Hafiz Muhammad Shoaib Shah, Muhammad Sohail Mazhar, Mahmood Ul Hasan, Andrew Woodward
Research outputs 2022 to 2026
Jackfruit (Artocarpus heterophyllus Lam.), also known as ‘vegetarian’s meat’, is an excellent source of carbohydrates, protein, fiber, vitamins, minerals, and several phytochemicals. It is a climacteric fruit that exhibits an increase in ethylene biosynthesis and respiration rate during fruit ripening. The market value of jackfruit is reduced due to the deterioration of fruit quality during storage and transportation. There is a lack of standardized harvest maturity index in jackfruit, where consequently, fruit harvested at immature or overmature stages result in poor quality ripe fruit with short storage life. Other factors responsible for its short postharvest life relate to its highly …
Melatonin Application Suppresses Oxidative Stress And Maintains Fruit Quality Of Cold Stored ‘Esperanza’ Raspberries By Regulating Antioxidant System, Hafiz M. S. Shah, Zora Singh, Mahmood Ul Hasan, Jashanpreet Kaur, Eben Afrifa-Yamoah, Andrew Woodward
Melatonin Application Suppresses Oxidative Stress And Maintains Fruit Quality Of Cold Stored ‘Esperanza’ Raspberries By Regulating Antioxidant System, Hafiz M. S. Shah, Zora Singh, Mahmood Ul Hasan, Jashanpreet Kaur, Eben Afrifa-Yamoah, Andrew Woodward
Research outputs 2022 to 2026
Raspberries are highly perishable and have a short storage life. To extend its storage life, we evaluated the impact of preharvest melatonin (MEL) application (0, 50, 100, 200 mol L−1), three days before harvest, on the activities of oxidative and antioxidative enzymes and quality attributes in ‘Esperanza’ raspberries up to 10 d cold storage. The 200 mol L−1 MEL-treated raspberries exhibited a significant reduction in disease incidence while maintaining higher marketable fruit and activities of superoxide dismutase, glutathione reductase, dehydroascorbate reductase, monodehydroascorbate reductase, ascorbate peroxidase and catalase as compared to control. Additionally, it reduced activities of polyphenol oxidase, peroxidase and …
Water Loss: A Postharvest Quality Marker In Apple Storage, Mahmood Ul Hasan, Zora Singh, Hafiz M. S. Shah, Jashanpreet Kaur, Andrew Woodward
Water Loss: A Postharvest Quality Marker In Apple Storage, Mahmood Ul Hasan, Zora Singh, Hafiz M. S. Shah, Jashanpreet Kaur, Andrew Woodward
Research outputs 2022 to 2026
Apple fruit can be stored for long periods of time, especially with the use of controlled atmosphere storage, but like many fruits and vegetables are susceptible to water loss. Water loss can result in compromised appearance such as skin shriveling, as well as loss of firmness, and reduced saleable weight, which in turn affect the income of growers and other industry stakeholders. Preharvest factors that can influence water loss in apples during the postharvest period include climate, cultivar, fruit size, tree age, orchard practices, and harvest maturity. Postharvest factors such as the storage temperature, relative humidity, storage type, and duration …
The Allergen Profile Of Two Edible Insect Species—Acheta Domesticus And Hermetia Illucens, Shaymaviswanathan Karnaneedi, Elecia B. Johnston, Utpal Bose, Angéla Juhász, James A. Broadbent, Thimo Ruethers, Emily M. Jerry, Sandip D. Kamath, Vachiranee Limviphuvadh, Sally Stockwell, Keren Byrne, Dean Clarke, Michelle L. Colgrave, Sebastian Maurer-Stroh, Andreas L. Lopata
The Allergen Profile Of Two Edible Insect Species—Acheta Domesticus And Hermetia Illucens, Shaymaviswanathan Karnaneedi, Elecia B. Johnston, Utpal Bose, Angéla Juhász, James A. Broadbent, Thimo Ruethers, Emily M. Jerry, Sandip D. Kamath, Vachiranee Limviphuvadh, Sally Stockwell, Keren Byrne, Dean Clarke, Michelle L. Colgrave, Sebastian Maurer-Stroh, Andreas L. Lopata
Research outputs 2022 to 2026
Scope: Edible insect proteins are increasingly introduced as an alternative sustainable food source to address the world's need to feed the growing population. Tropomyosin is the main insect allergen; however, additional potential allergens are not well characterized and the impact of extraction procedures on immunological reactivity is unknown. Methods and results: Proteins from different commercial food products derived from cricket (Acheta domesticus) and black soldier fly (BSF) (Hermetia illucens) are extracted using five different extraction buffers. The proteins are analyzed by SDS-PAGE and immunoblotting using allergen-specific antibodies and crustacean allergic patient sera. IgE binding bands are analyzed by mass spectrometry …
From Grain To Malt: Tracking Changes Of Ultra-Low-Gluten Barley Storage Proteins After Malting, Mahya Bahmani, Angéla Juhász, Utpal Bos, Mitchell G. Nye-Wood, Malcolm Blundell, Crispin A. Howitt, Michelle L. Colgrave
From Grain To Malt: Tracking Changes Of Ultra-Low-Gluten Barley Storage Proteins After Malting, Mahya Bahmani, Angéla Juhász, Utpal Bos, Mitchell G. Nye-Wood, Malcolm Blundell, Crispin A. Howitt, Michelle L. Colgrave
Research outputs 2022 to 2026
Barley (Hordeum vulgare L.) is a major cereal crop produced globally. Hordeins, the major storage proteins in barley, can trigger immune responses leading to celiac disease or symptoms associated with food allergy. Here, proteomics approaches were employed to investigate the proteome level changes of grain and malt from the malting barley cultivar, Sloop, and single-, double- and triple hordein-reduced lines. The triple hordein-reduced line is an ultra-low gluten barley cultivar, Kebari®. Using discovery proteomics, 2,688 and 3,034 proteins in the barley and malt samples were detected respectively. Through the application of targeted proteomics, a significant reduction in the quantity …
Oxalic Acid: A Blooming Organic Acid For Postharvest Quality Preservation Of Fresh Fruit And Vegetables, Mahmood Ul Ul Hasan, Zora Singh, Hafiz Muhammad Shoaib Shah, Jashanpreet Kaur, Andrew Woodward, Eben Afrifa-Yamoah, Aman Ullah Malik
Oxalic Acid: A Blooming Organic Acid For Postharvest Quality Preservation Of Fresh Fruit And Vegetables, Mahmood Ul Ul Hasan, Zora Singh, Hafiz Muhammad Shoaib Shah, Jashanpreet Kaur, Andrew Woodward, Eben Afrifa-Yamoah, Aman Ullah Malik
Research outputs 2022 to 2026
Oxalic acid (OA) is a unique organic acid that commonly occurs in plants with distinct functions in modulating metabolic processes. To date, the role of OA has primarily been studied in the deactivation of copper-containing preservatives, detoxification of aluminium toxicity and remediation of organic pollutants. During the last two decades, OA has been considered as an antioxidant compound with focus on its potential to enhance crop yield, improve fruit quality, boost nutritional profile, and delay postharvest senescence in fruit and vegetables. It has been established that pre- and postharvest OA application delays ripening and senescence by down-regulating physiological processes such …
Conventional Solid-State Fermentation Impacts The White Lupin Proteome Reducing The Abundance Of Allergenic Peptides, Arineh Tahmasian, Roger Drew, James A, Broadbent, Angéla Juhász, Mitchell Nye-Wood, Michelle L. Colgrave
Conventional Solid-State Fermentation Impacts The White Lupin Proteome Reducing The Abundance Of Allergenic Peptides, Arineh Tahmasian, Roger Drew, James A, Broadbent, Angéla Juhász, Mitchell Nye-Wood, Michelle L. Colgrave
Research outputs 2022 to 2026
The demand for high-quality and sustainable protein sources is on the rise. Lupin is an emerging plant-based source of protein with health-enhancing properties; however, the allergenic potential of lupins limits their widespread adoption in food products. A combination of discovery and targeted quantitative proteome measurements was used to investigate the impact of solid-state fermentation induced by Rhizopus oligosporus on the proteome composition and allergenic protein abundances of white lupin seed. In total, 1,241 proteins were uniquely identified in the fermented sample. Moreover, the effectiveness of the solid-state fermentation in reducing the abundance of the tryptic peptides derived from white lupin …
Nutrition, Allergenicity And Physicochemical Qualities Of Food-Grade Protein Extracts From Nannochloropsis Oculata, Sara Hamzelou, Damien Belobrajdic, Angéla Juhász, Henri Brook, Utpal Bose, Michelle L. Colgrave, James A. Broadbent
Nutrition, Allergenicity And Physicochemical Qualities Of Food-Grade Protein Extracts From Nannochloropsis Oculata, Sara Hamzelou, Damien Belobrajdic, Angéla Juhász, Henri Brook, Utpal Bose, Michelle L. Colgrave, James A. Broadbent
Research outputs 2022 to 2026
Microalgae offer an opportunity to act as a sustainable source of dietary protein. This study aimed to evaluate the impact of different protein extraction methods on the nutritional and physicochemical properties of Nannochloropsis oculata. Food-grade protein extracts were obtained by hypotonic osmotic shock using milli-Q water. Food grade (FG) and non-food grade (NFG) extraction buffers were compared along with three cell disruption methods including bead beating, probe sonication and a combination of both methods for protein extraction. Mass spectrometry was used for protein and putative allergen identification in FG extracts. Bead beating led to a slightly higher number of identifiable …
Low Gluten Beers Contain Variable Gluten And Immunogenic Epitope Content, Mitchell G. Nye-Wood, Keren Byrne, Sally Stockwell, Angéla Juhász, Utpal Bose, Michelle L. Colgrave
Low Gluten Beers Contain Variable Gluten And Immunogenic Epitope Content, Mitchell G. Nye-Wood, Keren Byrne, Sally Stockwell, Angéla Juhász, Utpal Bose, Michelle L. Colgrave
Research outputs 2022 to 2026
Gluten content labels inform food choice and people practicing a gluten-free diet rely upon them to avoid illness. The regulations differ between jurisdictions, especially concerning fermented foodstuffs such as beer. Gluten abundance is typically measured using ELISAs, which have come into question when testing fermented or hydrolysed foodstuffs such as beer. Mass spectrometry can be used to directly identify gluten peptides and reveal false negatives recorded by ELISA. In this survey of gluten in control and gluten-free beers, gluten protein fragments that contain known immunogenic epitopes were detected using liquid chromatography-mass spectrometry in multiple beers that claim to be gluten-free …
Safe Food Through Better Labelling: A Robust Method For The Rapid Determination Of Caprine And Bovine Milk Allergens, Joost L. D. Nelis, Amanda L. Dawson, Utpal Bose, Alisha Anderson, Michelle L. Colgrave, James A. Broadbent
Safe Food Through Better Labelling: A Robust Method For The Rapid Determination Of Caprine And Bovine Milk Allergens, Joost L. D. Nelis, Amanda L. Dawson, Utpal Bose, Alisha Anderson, Michelle L. Colgrave, James A. Broadbent
Research outputs 2022 to 2026
Accidental milk cross-contamination is one of the most common causes for costly food recalls. Yet, quantifying trace-levels of allergen is time-consuming and current methods are not adapted for routine analyses making quality control for trace-level allergen content impractical. This perpetuates voluntary “may-contain” statements that are unhelpful for people suffering from food allergies. Here, we developed a rapid LC-MS method enabling milk allergen quantification by comparing all tryptic-peptides of major milk allergens. The bovine-specific αS-2 casein peptide and allergen-epitope NAVPITPTLNR provided excellent performance in sensitivity (LOD 1 mg.kg − 1; LOQ 2 mg.kg − 1) across various dairy products, …
Higher Overall Intakes Are The Defining Feature Of Dietary Intakes In Nafld And Compared To The General Population, Catherine Properzi, Leon A. Adams, Johnny Lo, Jill L. Sherriff, Gary P. Jeffrey, Therese A. O’Sullivan
Higher Overall Intakes Are The Defining Feature Of Dietary Intakes In Nafld And Compared To The General Population, Catherine Properzi, Leon A. Adams, Johnny Lo, Jill L. Sherriff, Gary P. Jeffrey, Therese A. O’Sullivan
Research outputs 2022 to 2026
We aimed to compare the dietary intakes of Australian patients with non-alcoholic fatty liver disease (NAFLD) to general Australian population intake data and determine whether the intake of any nutrient or food group was able to predict the degree of steatosis. Dietary data from fifty adult patients with NAFLD were compared to intake data from the Australian Health Survey for energy, macronutrients, fat sub-types, alcohol, iron, folate, sugar, fibre, sodium and caffeine. Linear regression models adjusting for potential confounders (age, sex, physical activity and body mass index) were used to examine predictive relationships between hepatic steatosis (quantified via magnetic resonance …
Postharvest Biology And Technology Of Loquat (Eriobotrya Japonica Lindl.), Hafiz Muhammad Shoaib Shah, Ahmad Sattar Khan, Zora Singh, Saqib Ayyub
Postharvest Biology And Technology Of Loquat (Eriobotrya Japonica Lindl.), Hafiz Muhammad Shoaib Shah, Ahmad Sattar Khan, Zora Singh, Saqib Ayyub
Research outputs 2022 to 2026
Loquat (Eriobotrya japonica Lindl.) fruit is a rich source of carotenoids, flavonoids, phenolics, sugars, and organic acids. Although it is classified as a non-climacteric fruit, susceptibility to mechanical and physical bruising causes its rapid deterioration by moisture loss and postharvest decay caused by pathogens. Anthracnose, canker, and purple spot are the most prevalent postharvest diseases of loquat fruit. Cold storage has been used for quality management of loquat fruit, but the susceptibility of some cultivars to chilling injury (CI) consequently leads to browning and other disorders. Various techniques, including cold storage, controlled atmosphere storage, hypobaric storage, modified atmosphere packaging, low-temperature …
Unpacking The Proteome And Metaproteome Of The Black Soldier Fly Larvae: Efficacy And Complementarity Of Multiple Protein Extraction Protocols, Utpal Bose, Angela Juhasz, Sally Stockwell, Sophia Escobar-Correas, Anna Marcora, Cate Paull, James A. Broadbent, Gene Wijffels
Unpacking The Proteome And Metaproteome Of The Black Soldier Fly Larvae: Efficacy And Complementarity Of Multiple Protein Extraction Protocols, Utpal Bose, Angela Juhasz, Sally Stockwell, Sophia Escobar-Correas, Anna Marcora, Cate Paull, James A. Broadbent, Gene Wijffels
Research outputs 2022 to 2026
The larvae of the black soldier fly (BSF), Hermetia illucens (Diptera: Stratiomyidae), have demonstrated the ability to efficiently bioconvert organic waste into a sustainable source of food and feed, but fundamental biology remains to be discovered to exploit their full biodegradative potential. Herein, LC-MS/MS was used to assess the efficiency of eight differing extraction protocols to build foundational knowledge regarding the proteome landscape of both the BSF larvae body and gut. Each protocol yielded complementary information to improve BSF proteome coverage. Protocol 8 (liquid nitrogen, defatting, and urea/thiourea/chaps) was better than all other protocols for the protein extraction from larvae …
The Kraft Heinz Company Global Nutrition Targets For The Innovation And Reformulation Of Food And Beverages: Current And Future Directions, Lynn Yu, Véronique Braesco, Sheri L. Cooper, Adam Drewnowski, Bridget Hannon Esteves, Andrea Luigi Budelli
The Kraft Heinz Company Global Nutrition Targets For The Innovation And Reformulation Of Food And Beverages: Current And Future Directions, Lynn Yu, Véronique Braesco, Sheri L. Cooper, Adam Drewnowski, Bridget Hannon Esteves, Andrea Luigi Budelli
Research outputs 2022 to 2026
Reformulating packaged foods has the potential to improve the nutrient density of the global diet. The present perspective illustrates The Kraft Heinz Company’s approach to product (re)formulation to develop healthier product lines that are lower in saturated fats, total sugars, and sodium, and contain health promoting components. Here we present the rationale for The Kraft Heinz Company’s global nutrition targets used for the global innovation and renovation of foods and beverages. The global nutrition targets use a category specific approach to set maximum levels for the main nutrients of public health concern: saturated fat, total sugars and sodium, taking into …
Encapsulation Of Orange Peel Oil In Biopolymeric Nanocomposites To Control Its Release Under Different Conditions, Sanaz Ghasemi, Elham Assadpour, Mohammad Saeed Kharazmi, Shima Jafarzadeh, Masoumeh Zargar, Seid Mahdi Jafari
Encapsulation Of Orange Peel Oil In Biopolymeric Nanocomposites To Control Its Release Under Different Conditions, Sanaz Ghasemi, Elham Assadpour, Mohammad Saeed Kharazmi, Shima Jafarzadeh, Masoumeh Zargar, Seid Mahdi Jafari
Research outputs 2022 to 2026
Orange peel oil (OPO) is one of the most common flavorings used in the food industry, but it is volatile under environmental conditions (the presence of light, oxygen, humidity, and high temperatures). Encapsulation by biopolymer nanocomposites is a suitable and novel strategy to improve the bioavailability and stability of OPO and its controlled release. In this study, we investigated the release profile of OPO from freeze-dried optimized nanocomposite powders as a function of pH (3, 7, 11) and temperature (30, 60, and 90 °C), and within a simulated salivary system. Finally, its release kinetics modelling was performed using experimental models. …
Improving The Quality Of Set Yoghurt Using Milk Fat Globule Membrane Fragments, Joshua Ibitoye, T. T. Q. Phan, Duy N. Le, K. Dewettinck, Michelle L. Colgrave, A. P. Trzcinski, B. Ly-Nguyen
Improving The Quality Of Set Yoghurt Using Milk Fat Globule Membrane Fragments, Joshua Ibitoye, T. T. Q. Phan, Duy N. Le, K. Dewettinck, Michelle L. Colgrave, A. P. Trzcinski, B. Ly-Nguyen
Research outputs 2022 to 2026
Lacprodan PL20, a dairy ingredient that is rich in protein and polar lipids, was added into set yoghurts produced from nonhomogenized raw milk. The set yoghurts were prepared using concentrations of 2%, 4%, and 6% Lacprodan PL20, while the control sample was only supplemented with skim milk powder. The effect of Lacprodan PL20 concentrations on the physical and chemical properties, rheology, and microstructure of set yoghurt was thoroughly investigated to determine some likely improvement or changes in quality. Consequently, Lacprodan PL20 showed a gradual improvement in the set yoghurt nutritive values, water holding capacity, and apparent viscosity. The results indicated …
1 H-Cyclopropa[B]Naphthalene: A Novel Ethylene Antagonist For Extending Storage Life And Maintaining Quality Of Climacteric And Suppressed Climacteric Plums, Poe N. Kyaw, Zora Singh, Vijay Y. Tokala
1 H-Cyclopropa[B]Naphthalene: A Novel Ethylene Antagonist For Extending Storage Life And Maintaining Quality Of Climacteric And Suppressed Climacteric Plums, Poe N. Kyaw, Zora Singh, Vijay Y. Tokala
Research outputs 2022 to 2026
Ethylene is a key trigger that governs the rate of fruit ripening, storability, and postharvest quality of fresh fruit. Efficient inhibition of ethylene action is essential to slow down the postharvest ripening processes, extend storage life and maintain optimum fruit quality during storage. Ethylene antagonist compounds with the likelihood of application as liquid formulation would facilitate managing ethylene broadly as both preharvest and/or postharvest treatments. This study examined the effects of different aqueous formulations of 1 H-cyclopropa[b]naphthalene (NC) as an ethylene antagonist in the cold stored (1 °C) Japanese plums ‘Angeleno’ (suppressed-climacteric) and ‘Tegan Blue’, which exhibit climacteric peaks, respectively. …
Development Of A Scoring Tool For Australian Rural Food Retail Environments, Tracy L. Schumacher, Carissa A. Alderton, Leanne J. Brown, Susan Heaney, Laura Alston, Katherine Kent, Stephanie L. Godrich
Development Of A Scoring Tool For Australian Rural Food Retail Environments, Tracy L. Schumacher, Carissa A. Alderton, Leanne J. Brown, Susan Heaney, Laura Alston, Katherine Kent, Stephanie L. Godrich
Research outputs 2022 to 2026
Current tools scoring the healthiness of food retail outlets do not reflect outlets found in rural locations. This study aimed to adapt pre-existing Australian scoring tools to represent non-metropolitan areas. Rural nutrition experts were identified, and a modified Delphi technique was used to adapt two pre-existing, food-scoring tools in five iterative stages. Stages included identifying all relevant outlets, providing a description and score for each, ensuring consistency between outlet scores and pre-existing, metro-centric tools, and providing instructions for correct use. Six rural nutrition experts were identified and engaged in the modified Delphi technique. The final tool consisted of 12 categories …
Trends In Maintaining Postharvest Freshness And Quality Of Rubus Berries, Hafiz Muhammad Shoaib Shah, Zora Singh, Jashanpreet Kaur, Mahmood Ul Hasan, Andrew Woodward, Eben Afrifa-Yamoah
Trends In Maintaining Postharvest Freshness And Quality Of Rubus Berries, Hafiz Muhammad Shoaib Shah, Zora Singh, Jashanpreet Kaur, Mahmood Ul Hasan, Andrew Woodward, Eben Afrifa-Yamoah
Research outputs 2022 to 2026
Blackberries and raspberries, commonly known as Rubus berries, are commercially grown worldwide across different climates. Rubus berries contain wide array of phytochemicals, vitamins, dietary fibers, minerals, and unsaturated fatty acids. Nevertheless, these berries have short storage life which is the major constraint in their supply chains leading to higher postharvest losses. Inappropriate harvest handling, physical bruising, insect pests, and postharvest diseases lower the acceptability of fruit among consumers and other supply chain stakeholders. Additionally, the susceptibility to microbial decay, fruit softening, higher ethylene production, respiratory activity, and increased oxidation of anthocyanins, phenolics, and flavonoids considerably affects the marketability of Rubus …
Utilizing Proteomics To Identify And Optimize Microalgae Strains For High-Quality Dietary Protein: A Review, Sara Hamzelou, Damien Belobrajdic, James A. Broadbent, Angéla Juhász, Kim L. Chang, Ian Jameson, Peter Ralph, Michelle L. Colgrave
Utilizing Proteomics To Identify And Optimize Microalgae Strains For High-Quality Dietary Protein: A Review, Sara Hamzelou, Damien Belobrajdic, James A. Broadbent, Angéla Juhász, Kim L. Chang, Ian Jameson, Peter Ralph, Michelle L. Colgrave
Research outputs 2022 to 2026
Algae-derived protein has immense potential to provide high-quality protein foods for the expanding human population. To meet its potential, a broad range of scientific tools are required to identify optimal algal strains from the hundreds of thousands available and identify ideal growing conditions for strains that produce high-quality protein with functional benefits. A research pipeline that includes proteomics can provide a deeper interpretation of microalgal composition and biochemistry in the pursuit of these goals. To date, proteomic investigations have largely focused on pathways that involve lipid production in selected microalgae species. Herein, we report the current state of microalgal proteome …
Plant Proteomic Research 5.0: From Data To Insights, Setsuko Komatsu, Michelle L. Colgrave
Plant Proteomic Research 5.0: From Data To Insights, Setsuko Komatsu, Michelle L. Colgrave
Research outputs 2022 to 2026
Proteomics offers one of the best approaches for the functional analysis of the genome, generating detailed information that can be integrated with that obtained by other classic and omics approaches. It, thus, provides deep knowledge and an understanding of diverse proteoforms and different plant processes. Several different generations of proteomic platforms have appeared in the past 25 years. They have been exploited for describing protein profiles, post-translational modifications, and subcellular localization. Despite recent advancements, more emphasis needs to be given to the protein sample preparation protocols, especially for cases with very low abundance, hydrophobicity, and a large molecular weight. The …
Maturity Stage At Harvest Influences Antioxidant Phytochemicals And Antibacterial Activity Of Jujube Fruit (Ziziphus Mauritiana Lamk. And Ziziphus Spina-Christi L.), Adhbah R. H. Aldhanhani, Zienab F. R. Ahmed, Nikolaos Tzortzakis, Zora Singh
Maturity Stage At Harvest Influences Antioxidant Phytochemicals And Antibacterial Activity Of Jujube Fruit (Ziziphus Mauritiana Lamk. And Ziziphus Spina-Christi L.), Adhbah R. H. Aldhanhani, Zienab F. R. Ahmed, Nikolaos Tzortzakis, Zora Singh
Research outputs 2022 to 2026
Fruits of the genus Ziziphus are known for their medicinal and nutritional value. Thus, this study aimed to investigate the influence of different maturity stages (unripe, half-ripe, and ripe fruit) at harvest on the physicochemical characteristics, phytochemical composition, antioxidants, and antibacterial activities of Ziziphus mauritiana and Ziziphus spina-christi fruits. In both plant species, the unripe fruits had significantly higher (P < 0.05) phenolic and flavonoid content and antioxidant and antibacterial activity compared with ripe fruits. At all maturity stages, these features were significantly higher (P < 0.05) in Z. spina-christi fruits than in Z. mauritiana fruits. Regardless of the species, 13 phenolic compounds were identified and quantified in the fruit extracts. For both species, the concentration of individual phenolic compounds changed with fruit maturity. Among the phenolic compounds, the gallic acid, 4-hydroxybenzoic acid, salicylic acid, catechin, and rutin levels were significantly higher (P < 0.05) in Z. spina-christi fruits than those for Z. mauritiana fruits, whereas vanillic acid and quercetin showed the opposite trend. Additionally, trans-cinnamic acid was only detected in Z. spina-christi fruits. In conclusion, the mature unripe jujube fruit, especially in Z. spina-christi, was shown to be a promising source of various naturally occurring compounds of significant interest owing to its pharmacological and therapeutic properties as an antioxidant, anti-inflammatory, and analgesic.
Spectrophotometric Methods For Measurement Of Antioxidant Activity In Food And Pharmaceuticals, Marios C. Christodoulou, Jose C. Orellana Palacios, Golnaz Hesami, Shima Jafarzadeh, José M. Lorenzo, Rubén Domínguez, Andres Moreno, Milad Hadidi
Spectrophotometric Methods For Measurement Of Antioxidant Activity In Food And Pharmaceuticals, Marios C. Christodoulou, Jose C. Orellana Palacios, Golnaz Hesami, Shima Jafarzadeh, José M. Lorenzo, Rubén Domínguez, Andres Moreno, Milad Hadidi
Research outputs 2022 to 2026
In recent years, there has been a growing interest in the application of antioxidants in food and pharmaceuticals due to their association with beneficial health effects against numerous oxidative-related human diseases. The antioxidant potential can be measured by various assays with specific mechanisms of action, including hydrogen atom transfer, single electron transfer, and targeted scavenging activities. Understanding the chemistry of mechanisms, advantages, and limitations of the methods is critical for the proper selection of techniques for the valid assessment of antioxidant activity in specific samples or conditions. There are various analytical techniques available for determining the antioxidant activity of biological …
Proteome Phenotypes Discriminate The Growing Location And Malting Traits In Field-Grown Barley, Mahya Bahmani, Angéla Juhász, James Broadbent, Utpal Bose, Mitchell G. Nye-Wood, Ian B. Edwards, Michelle L. Colgrave
Proteome Phenotypes Discriminate The Growing Location And Malting Traits In Field-Grown Barley, Mahya Bahmani, Angéla Juhász, James Broadbent, Utpal Bose, Mitchell G. Nye-Wood, Ian B. Edwards, Michelle L. Colgrave
Research outputs 2022 to 2026
Barley is one of the key cereal grains for malting and brewing industries. However, climate variability and unprecedented weather events can impact barley yield and end-product quality. The genetic background and environmental conditions are key factors in defining the barley proteome content and malting characteristics. Here, we measure the barley proteome and malting characteristics of three barley lines grown in Western Australia, differing in genetic background and growing location, by applying liquid chromatography-mass spectrometry (LC-MS). Using data-dependent acquisition LC-MS, 1571 proteins were detected with high confidence. Quantitative data acquired using sequential window acquisition of all theoretical (SWATH) MS on barley …
Digestibility Of Wheat Alpha-Amylase/Trypsin Inhibitors Using A Caricain Digestive Supplement, Angéla Juhász, Mitchell G. Nye-Wood, Gregory J. Tanner, Michelle L. Colgrave
Digestibility Of Wheat Alpha-Amylase/Trypsin Inhibitors Using A Caricain Digestive Supplement, Angéla Juhász, Mitchell G. Nye-Wood, Gregory J. Tanner, Michelle L. Colgrave
Research outputs 2022 to 2026
Wheat is a major source of nutrition, though in susceptible people it can elicit inappropriate immune responses. Wheat allergy and non-celiac wheat sensitivity are caused by various wheat proteins, including alpha-amylase trypsin inhibitors (ATIs). These proteins, like the gluten proteins which can cause celiac disease, are incompletely digested in the stomach such that immunogenic epitopes reach the lower digestive system where they elicit the undesirable immune response. The only completely effective treatment for these immune reactions is to eliminate the food trigger from the diet, though inadvertent or accidental consumption can still cause debilitating symptoms in susceptible people. One approach …