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Articles 601 - 630 of 1129

Full-Text Articles in Entomology

Fruit Fly Nutrition, Rearing And Quality Control, Samira A. Mohamed, Fathiya M. Khamis, Chrysantus M. Tanga Jan 2016

Fruit Fly Nutrition, Rearing And Quality Control, Samira A. Mohamed, Fathiya M. Khamis, Chrysantus M. Tanga

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Tephritid fruit flies are recognized worldwide as an important threat to the horticultural industry. Most of the species belonging to this group are highly polyphagous attacking several important fruits and vegetables. They cause direct damage through larval feeding and indirect losses are associated with quarantine restrictions. The increasing awareness of the damage caused by these fruit flies to the horticultural industry has created a demand for the development of control measures based on integrated pest management (IPM) strategies and the sterile insect technique (SIT). However, success of the majority of these control methods largely depends on the ability to establish …


Spatial Separation Of Semiochemical Lurem-Tr And Entomopathogenic Fungi To Enhance Their Compatibility And Infectivity In An Autoinoculation System For Thrips Management, David K. Mfuti, Sevgan Subramanian, Rob Whm Van Tol, Gerrie L. Wiegers, Willem J. De Kogel, Saliou Niassy, Hannalene Du Plessis, Sunday Ekesi, Nguya K. Maniania Jan 2016

Spatial Separation Of Semiochemical Lurem-Tr And Entomopathogenic Fungi To Enhance Their Compatibility And Infectivity In An Autoinoculation System For Thrips Management, David K. Mfuti, Sevgan Subramanian, Rob Whm Van Tol, Gerrie L. Wiegers, Willem J. De Kogel, Saliou Niassy, Hannalene Du Plessis, Sunday Ekesi, Nguya K. Maniania

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BACKGROUND: The effect of spatial separation of the semiochemical Lurem-TR, which has been found to inhibit conidia of entomopathogenic fungi when put together, on the persistence of conidia of Metarhizium brunneum and M. anisopliae was evaluated in the greenhouse and field in order to develop an autodissemination strategy for the management of Megalurothrips sjostedti on cowpea crop. Influence of spatial separation of the semiochemical on thrips attraction and conidial acquisition by thrips from the autoinoculation device was also investigated in the field. RESULTS: Persistence of conidia of M. brunneum and M. anisopliae increased with distance of separation of Lurem-TR. Direct …


Fruit Fly Species Composition, Distribution And Host Plants With Emphasis On Vegetable-Infesting Species, Chrysantus M. Tanga, Ivan Rwomushana Jan 2016

Fruit Fly Species Composition, Distribution And Host Plants With Emphasis On Vegetable-Infesting Species, Chrysantus M. Tanga, Ivan Rwomushana

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Vegetable crops hold a key position in smallholder agricultural production systems in Africa due to the number of farmers involved, income generation, employment opportunities, and enhancement of food and nutritional security. In many developed countries, demand for vegetables continues to grow due to increased awareness of the nutritional benefits, thereby stimulating increased domestic production and also imports from Africa. However, many pests threaten the productivity of the vegetable sector. Key amongst these are tephritid fruit flies that inflict both direct and indirect losses; alien invasive species are often responsible for severe ecological and economic impacts. The highly invasive and polyphagous …


Plant Resting Site Preferences And Parity Rates Among The Vectors Of Rift Valley Fever In Northeastern Kenya, Samwel O. Arum, Christopher W. Weldon, Benedict Orindi, Caroline Tigoi, Francis Musili, Tobias Landmann, David P. Tchouassi, Hippolyte D. Affognon, Rosemary Sang Jan 2016

Plant Resting Site Preferences And Parity Rates Among The Vectors Of Rift Valley Fever In Northeastern Kenya, Samwel O. Arum, Christopher W. Weldon, Benedict Orindi, Caroline Tigoi, Francis Musili, Tobias Landmann, David P. Tchouassi, Hippolyte D. Affognon, Rosemary Sang

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Background: Mosquito lifespan can influence the circulation of disease causing pathogens because it affects the time available for infection and transmission. The life-cycle of mosquitoes is determined by intrinsic and environmental factors, which can include the availability of hosts and suitable resting environments that shelter mosquitoes from extreme temperature and desiccating conditions. This study determined the parity rates (an indirect measure of survival) and plant resting preference of vectors of Rift Valley fever (RVF) in northeastern Kenya. Methods: Resting mosquitoes were trapped during the rainy and the dry season using a Prokopack aspirator from vegetation, whereas general adult populations were …


Mosquito Host Choices On Livestock Amplifiers Of Rift Valley Fever Virus In Kenya, David P. Tchouassi, Robinson O.K. Okiro, Rosemary Sang, Lee W. Cohnstaedt, David Scott Mcvey, Baldwyn Torto Jan 2016

Mosquito Host Choices On Livestock Amplifiers Of Rift Valley Fever Virus In Kenya, David P. Tchouassi, Robinson O.K. Okiro, Rosemary Sang, Lee W. Cohnstaedt, David Scott Mcvey, Baldwyn Torto

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Background: Animal hosts may vary in their attraction and acceptability as components of the host location process for assessing preference, and biting rates of vectors and risk of exposure to pathogens. However, these parameters remain poorly understood for mosquito vectors of the Rift Valley fever (RVF), an arboviral disease, and for a community of mosquitoes. Methods: Using three known livestock amplifiers of RVF virus including sheep, goat and cattle as bait in enclosure traps, we investigated the host-feeding patterns for a community of mosquitoes in Naivasha, an endemic area of Rift Valley fever (RVF), in a longitudinal study for six …


Megastigmus Seed Chalcids (Hymenoptera, Torymidae) Radiated Much More On Angiosperms Than Previously Considered. I- Description Of 8 New Species From Kenya, With A Key To The Females Of Eastern And Southern Africa, Alain Roques, Robert S. Copeland, Laurent Soldati, Olivier Denux, Marie Anne Auger-Rozenberg Jan 2016

Megastigmus Seed Chalcids (Hymenoptera, Torymidae) Radiated Much More On Angiosperms Than Previously Considered. I- Description Of 8 New Species From Kenya, With A Key To The Females Of Eastern And Southern Africa, Alain Roques, Robert S. Copeland, Laurent Soldati, Olivier Denux, Marie Anne Auger-Rozenberg

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A survey of seed chalcids from woody plants in Kenya revealed 12 species belonging to the genus Megastigmus Dalman, 1820, and has increased to 16 the number of Megastigmus species presently recorded from the Afrotropical Region, of which at least 13 are seed feeders. A key to female Megastigmus of the Afrotropical Region is provided. Eight new species are described from morphological evidence: M. lanneae Roques & Copeland, M. laventhali Roques & Copeland, M. ozoroae Roques & Copeland, and M. smithi Roques & Copeland in seeds of species of the family Anacardiaceae, M. copelandi Roques & Copeland and M. grewianae …


Active Aggregation Among Sexes In Bean Flower Thrips (Megalurothrips Sjostedti) On Cowpea (Vigna Unguiculata), Saliou Niassy, Sunday Ekesi, Nguya K. Maniania, Benedict Orindi, Gerald B. Moritz, Willem J. De Kogel, Sevgan Subramanian Jan 2016

Active Aggregation Among Sexes In Bean Flower Thrips (Megalurothrips Sjostedti) On Cowpea (Vigna Unguiculata), Saliou Niassy, Sunday Ekesi, Nguya K. Maniania, Benedict Orindi, Gerald B. Moritz, Willem J. De Kogel, Sevgan Subramanian

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Male sexual aggregations are a common territorial, mating-related or resource-based, behaviour observed in diverse organisms, including insects such as thrips. The influence of factors such as plant substrate, time of day, and geographic location on aggregation of thrips is uncertain, therefore we monitored the dispersion of male and female bean flower thrips (BFT), Megalurothrips sjostedti (Trybom) (Thysanoptera: Thripidae), on cowpea, Vigna unguiculata (L.) Walp. (Fabaceae), over three cowpea growth stages and across three cowpea-growing areas of Kenya. Our results indicated that for all the crop growth stages, the density of BFTs varied over the time of day, with higher densities …


Determination Of Pesticide Residues In Honey: A Preliminary Study From Two Of Africa’S Largest Honey Producers, Janet Irungu, Suresh Raina, Baldwyn Torto Jan 2016

Determination Of Pesticide Residues In Honey: A Preliminary Study From Two Of Africa’S Largest Honey Producers, Janet Irungu, Suresh Raina, Baldwyn Torto

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Background: The presence of pollutants in honey can influence honey bee colony performance and devalue its use for human consumption. Using liquid chromatography tandem mass spectrometry (LC-MS/MS), various clean-up methods were evaluated for efficient determination of multiclass pesticide contaminants in honey. The selected clean-up method was optimized and validated and then applied to perform a preliminary study of commercial honey samples from Africa. Results: The most efficient method was primary-secondary amine (PSA) sorbent which was significantly different from the others (P <0.05; average recovery ~94 %) and was applied to analyze 96 pesticide residues in 28 retail honey samples from Kenya and Ethiopia. From our preliminary data, a total of 17 pesticide residues were detected at ~10-fold below maximum residue limit (MRL) established for food products except for malathion which was detected at almost 2-fold above its acceptable MRL. Conclusions: A highly efficient approach for determining pesticide residues in honey with good recoveries was developed. All residue contaminants were detected at levels well below their acceptable MRLs except malathion suggesting that the retail honey analyzed is safe for human consumption. Although PSA clean-up method was selected as the most efficient for cleaning honey samples, omitting the clean-up step was the most economical approach with potential applicability in the food industry.


Some Key Elements On Entomophagy In Africa: Culture, Gender And Belief, S. Niassy, H. D. Affognon, K. K.M. Fiaboe, K. S. Akutse, C. M. Tanga, S. Ekesi Jan 2016

Some Key Elements On Entomophagy In Africa: Culture, Gender And Belief, S. Niassy, H. D. Affognon, K. K.M. Fiaboe, K. S. Akutse, C. M. Tanga, S. Ekesi

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Edible insects are a natural renewable food resource and, within the context of food security could be one solution for Africa. Although, entomophagy is still being practiced in Africa, there is a degree of rejection in certain milieu due to negative images associated with insects and poor awareness efforts. In order to understand the key elements that govern entomophagy in Africa, we conducted an online survey on edible insects in African countries and documented the importance of insects, cultures and believes associated to their use. We eventually looked at gender participation in entomophagy. Study showed that insect consumption can be …


Integrated Management Of Fruit Flies-Case Studies From Uganda, Brian E. Isabirye, Caroline K. Nankinga, Alex Mayamba, Anne M. Akol, Ivan Rwomushana Jan 2016

Integrated Management Of Fruit Flies-Case Studies From Uganda, Brian E. Isabirye, Caroline K. Nankinga, Alex Mayamba, Anne M. Akol, Ivan Rwomushana

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Fruit flies (Diptera: Tephritidae) pose a threat to commercialisation of the horticulture industry in Uganda. They impair the quality and quantity of fruits produced, and limit access to lucrative regional and global markets. Here we explore past and present efforts, and future plans for research and management, of fruit flies in Uganda. Early research geared towards collection and identification of fruit flies recognised the pest status of many species and highlighted the need for establishing sustainable management strategies. Subsequently large-scale research initiatives have substantially increased knowledge on the biology and ecology of fruit flies in Uganda. Based on these studies, …


Antagonistic Interactions Between The Africanweaver Ant Oecophylla Longinoda And The Parasitoid Anagyrus Pseudococci Potentially Limits Suppression Of The Invasive Mealybug Rastrococcus Iceryoides, Chrysantus M. Tanga, Sunday Ekesi, Prem Govender, Peterson W. Nderitu, Samira A. Mohamed Dec 2015

Antagonistic Interactions Between The Africanweaver Ant Oecophylla Longinoda And The Parasitoid Anagyrus Pseudococci Potentially Limits Suppression Of The Invasive Mealybug Rastrococcus Iceryoides, Chrysantus M. Tanga, Sunday Ekesi, Prem Govender, Peterson W. Nderitu, Samira A. Mohamed

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The ant Oecophylla longinoda Latreille forms a trophobiotic relationship with the invasive mealybug Rastrococus iceryoides Green and promotes the latter’s infestations to unacceptable levels in the presence of their natural enemies. In this regard, the antagonistic interactions between the ant and the parasitoid Anagyrus pseudococci Girault were assessed under laboratory conditions. The percentage of parasitism of R. iceryoides by A. pseudococci was significantly higher on “ant-excluded” treatments (86.6% ± 1.27%) compared to “ant-tended” treatments (51.4% ± 4.13%). The low female-biased sex-ratio observed in the “ant-tended” treatment can be attributed to ants’ interference during the oviposition phase, which disrupted parasitoids’ ability …


Eco-Friendly Nets To Improve Vegetable Production And Quality In Sub-Saharan Africa, T. Martin, S. Simon, L. Parrot, F. Assogba Komlan, F. Vidogbena, A. Adegbidi, V. Baird, M. Saidi, M. Kasina, L. A. Wasilwa, S. Subramanian, M. Ngouajio Dec 2015

Eco-Friendly Nets To Improve Vegetable Production And Quality In Sub-Saharan Africa, T. Martin, S. Simon, L. Parrot, F. Assogba Komlan, F. Vidogbena, A. Adegbidi, V. Baird, M. Saidi, M. Kasina, L. A. Wasilwa, S. Subramanian, M. Ngouajio

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Continuous coverage of vegetables with nets under different climate conditions in Benin and Kenya proved effective in protecting vegetable nurseries and main fields against key lepidopteran and dipteran pests. However, netting did not effectively protect the vegetables against small insects such as aphids and whiteflies, although a visual barrier could delay outbreaks. Hence, opening the nets during the day could be an alternative option to conserve the natural enemies for small insects. Moreover, the efficacy of the visual barrier may be complemented by a repellent compound impregnated in the net. The small-holder growers adopting the nets in Benin and Kenya …


Genetic Divergence Of Chikungunya Virus Plaque Variants From The Comoros Island (2005), Caroline Wasonga, Shingo Inoue, Cecilia Rumberia, George Michuki, James Kimotho, Juliette R. Ongus, Rosemary Sang, Lillian Musila Dec 2015

Genetic Divergence Of Chikungunya Virus Plaque Variants From The Comoros Island (2005), Caroline Wasonga, Shingo Inoue, Cecilia Rumberia, George Michuki, James Kimotho, Juliette R. Ongus, Rosemary Sang, Lillian Musila

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Chikungunya virus (CHIKV) from a human sample collected during the 2005 Chikungunya outbreak in the Comoros Island, showed distinct and reproducible large (L2) and small (S7) plaques which were characterized in this study. The parent strain and plaque variants were analysed by in vitro growth kinetics in different cell lines and their genetic similarity assessed by whole genome sequencing, comparative sequence alignment and phylogenetic analysis. In vitro growth kinetic assays showed similar growth patterns of both plaque variants in Vero cells but higher viral titres of S7 compared to L2 in C6/36 cells. Amino acids (AA) alignments of the CHIKV …


Cost: Benefit Analysis Of Insect Net Use In Cabbage In Real Farming Conditions Among Smallholder Farmers In Benin, Faustin Vidogbéna, Anselme Adégbidi, Françoise Assogba-Komlan, Thibaud Martin, Mathieu Ngouajio, Serge Simon, Rigobert Tossou, Laurent Parrot Dec 2015

Cost: Benefit Analysis Of Insect Net Use In Cabbage In Real Farming Conditions Among Smallholder Farmers In Benin, Faustin Vidogbéna, Anselme Adégbidi, Françoise Assogba-Komlan, Thibaud Martin, Mathieu Ngouajio, Serge Simon, Rigobert Tossou, Laurent Parrot

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Insect net use provides a physical exclusion of pests as well as a microclimate change, thus increasing soil humidity, vegetable quality and yields in tropical conditions. This paper presents the findings of a cost:benefit analysis of this technology in real farming conditions compared to current insecticide practices. The surveys were conducted in Benin for cabbage production (. Brassica oleracea L.) during two production cycles. There were almost no significant differences in costs between unnetted and netted cabbage for the two production cycles. The only factors that varied were farm gate prices, the number of cabbages marketed and their size. Improved …


An Improved Odor Bait For Monitoring Populations Of Aedes Aegypti-Vectors Of Dengue And Chikungunya Viruses In Kenya, Eunice A. Owino, Rosemary Sang, Catherine L. Sole, Christian Pirk, Charles Mbogo, Baldwyn Torto Dec 2015

An Improved Odor Bait For Monitoring Populations Of Aedes Aegypti-Vectors Of Dengue And Chikungunya Viruses In Kenya, Eunice A. Owino, Rosemary Sang, Catherine L. Sole, Christian Pirk, Charles Mbogo, Baldwyn Torto

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Abstract Background: Effective surveillance and estimation of the biting fraction of Aedes aegypti is critical for accurate determination of the extent of virus transmission during outbreaks and inter-epidemic periods of dengue and chikungunya fever. Here, we describe the development and use of synthetic human odor baits for improved sampling of adult Ae. aegypti, in two dengue and chikungunya fevers endemic areas in Kenya; Kilifi and Busia counties. Methods: We collected volatiles from the feet and trunks of two female and two male volunteers aged between 25 and 45 years. We used coupled gas chromatography- electroantennographic detection (GC/EAD) analysis to screen …


Olfactory Associative Learning In Two African Stingless Bee Species (Meliponula Ferruginea And M. Bocandei, Meliponini), J. Henske, K. Krausa, F. A. Hager, K. Nkoba, W. H. Kirchner Nov 2015

Olfactory Associative Learning In Two African Stingless Bee Species (Meliponula Ferruginea And M. Bocandei, Meliponini), J. Henske, K. Krausa, F. A. Hager, K. Nkoba, W. H. Kirchner

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Worldwide only a few of the more than 500 stingless bee species has been studied in any detail. Most studies on stingless bees have been conducted in the Neotropics, whereas a very few have been undertaken in Africa. Foraging success is dependent to a greater or lesser extent on olfactory cues or signals. A prerequisite to effective foraging via odors is that the bees are able to associate odors with a nectar reward and memorize this information. In the context of olfactory learning, only five species of stingless bees have been studied using classical conditioning of the proboscis extension reflex …


A New Species Of Alhajarmyia Stuckenberg (Diptera: Vermileonidae), The First Wormlion Fly Described From East Africa And Its Biogeographical Implications, Vaughn R. Swart, Ashley H. Kirk-Spriggs, Robert S. Copeland Nov 2015

A New Species Of Alhajarmyia Stuckenberg (Diptera: Vermileonidae), The First Wormlion Fly Described From East Africa And Its Biogeographical Implications, Vaughn R. Swart, Ashley H. Kirk-Spriggs, Robert S. Copeland

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A second species of the genus Alhajarmyia Stuckenberg (A. stuckenbergi Swart, Kirk-Spriggs & Copeland, sp. n.), is described and figured, from the Eastern Arc Mountains of Kenya (Kasigau Mountain and Taita Hills), being the first vermileonid recorded from East Africa. The species is shown to differ from its congener, A. umbraticola (Stuckenberg & Fisher), described from Oman in the Arabian Peninsula, based on external characters including male and female terminalia. An identification key is provided together with distribution maps for the two species, and biogeographical aspects are discussed.


Linalool Oxide: Generalist Plant Based Lure For Mosquito Disease Vectors, Vincent O. Nyasembe, David P. Tchouassi, Charles M. Mbogo, Catherine L. Sole, Christian Pirk, Baldwyn Torto Nov 2015

Linalool Oxide: Generalist Plant Based Lure For Mosquito Disease Vectors, Vincent O. Nyasembe, David P. Tchouassi, Charles M. Mbogo, Catherine L. Sole, Christian Pirk, Baldwyn Torto

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Background: Lack of effective vaccines and therapeutics for important arboviral diseases such as Rift Valley fever (RVF) and dengue, necessitates continuous monitoring of vector populations for infections in them. Plant-based lures as surveillance tools has the potential of targeting mosquitoes of both sexes and females of varied physiological states; yet such lures are lacking for vectors of these diseases. Here, we present evidence of the effectiveness of linalool oxide (LO), a single plant-based lure previously developed for malaria vectors in trapping RVF vectors, Aedes mcintoshi and Aedes ochraceus, and dengue vector, Aedes aegypti. Methods: For RVF vectors, we used CDC …


Effects Of Agroforestry On Pest, Disease And Weed Control: A Meta-Analysis, Lorena Pumariño, Gudeta Weldesemayat Sileshi, Sofia Gripenberg, Riikka Kaartinen, Edmundo Barrios, Mary Nyawira Muchane, Charles Midega, Mattias Jonsson Nov 2015

Effects Of Agroforestry On Pest, Disease And Weed Control: A Meta-Analysis, Lorena Pumariño, Gudeta Weldesemayat Sileshi, Sofia Gripenberg, Riikka Kaartinen, Edmundo Barrios, Mary Nyawira Muchane, Charles Midega, Mattias Jonsson

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Agroforestry practices may influence pest incidence and abundance both through increased top-down regulation by natural enemies and via bottom-up factors such as moderation of microclimate, soil nutrients and water content. We conducted a meta-analysis of the effects of agroforestry on the abundance of invertebrate pests, weeds, natural enemies and plant damage due to pests and diseases. We also tested whether effects of agroforestry were dependent on crop type (annual or perennial), type of pest association (above or belowground) and weed type (parasitic Striga weeds or non-parasitic weeds).Agroforestry practices resulted in lower abundances of both parasitic and non-parasitic weeds, and in …


Differential Expression Of The Chemosensory Transcriptome In Two Populations Of The Stemborer Sesamia Nonagrioides, Nicolas Glaser, Aurore Gallot, Fabrice Legeai, Myriam Harry, Laure Kaiser, Bruno Le Ru, Paul André Calatayud, Emmanuelle Jacquin-Joly Oct 2015

Differential Expression Of The Chemosensory Transcriptome In Two Populations Of The Stemborer Sesamia Nonagrioides, Nicolas Glaser, Aurore Gallot, Fabrice Legeai, Myriam Harry, Laure Kaiser, Bruno Le Ru, Paul André Calatayud, Emmanuelle Jacquin-Joly

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Among the proposed mechanisms of local adaptation to different ecological environments, transcriptional changes may play an important role. In this study, we investigated whether such variability occurred within the chemosensory organs of a herbivorous insect, for which chemosensation guides most of its host preferences. A European and an African population of the noctuid Sesamia nonagrioides that display significant differences in their ecological preferences were collected on Zea mays and Typha domingensis, respectively. RNAseq were used between the two populations for digital expression profiling of chemosensory organs from larval antennae and palps. Preliminary data on adult female antennae and ovipositors were …


Sternal Gland Structures In Males Of Bean Flower Thrips, Megalurothrips Sjostedti, And Poinsettia Thrips, Echinothrips Americanus, In Comparison With Those Of Western Flower Thrips, Frankliniella Occidentalis (Thysanoptera: Thripidae), Stephanie Krueger, Sevgan Subramanian, Saliou Niassy, Gerald B. Moritz Sep 2015

Sternal Gland Structures In Males Of Bean Flower Thrips, Megalurothrips Sjostedti, And Poinsettia Thrips, Echinothrips Americanus, In Comparison With Those Of Western Flower Thrips, Frankliniella Occidentalis (Thysanoptera: Thripidae), Stephanie Krueger, Sevgan Subramanian, Saliou Niassy, Gerald B. Moritz

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Sternal pores are important features for identification of male thrips, especially within the subfamily Thripinae. They vary in shape, size and distribution even between species of one genus. Their functional role is speculated to be that of sex- and/or aggregation pheromone production. Yet, sexual aggregations are not reported in Echinothrips americanus, known to have sternal pores, while we observed aggregations in Megalurothrips sjostedti, previously reported to lack them. We examined the sternal glands and pores of the thripine species E. americanus and M. sjostedti males, in comparison with those of Frankliniella occidentalis using light microscopy, as well as scanning and …


Influence Of Dietary Experience On The Induction Of Preference Of Adult Moths And Larvae For A New Olfactory Cue, Christophe Petit, Bruno Le Ru, Stéphane Dupas, Brigitte Frérot, Peter Ahuya, Laure Kaiser-Arnauld, Myriam Harry, Paul André Calatayud Aug 2015

Influence Of Dietary Experience On The Induction Of Preference Of Adult Moths And Larvae For A New Olfactory Cue, Christophe Petit, Bruno Le Ru, Stéphane Dupas, Brigitte Frérot, Peter Ahuya, Laure Kaiser-Arnauld, Myriam Harry, Paul André Calatayud

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In Lepidoptera, host plant selection is first conditioned by oviposition site preference of adult females followed by feeding site preference of larvae. Dietary experience to plant volatile cues can induce larval and adult host plant preference. We investigated how the parent's and self-experience induce host preference in adult females and larvae of three lepidopteran stem borer species with different host plant ranges, namely the polyphagous Sesamia nonagrioides, the oligophagous Busseola fusca and the monophagous Busseola nairobica, and whether this induction can be linked to a neurophysiological phenotypic plasticity. The three species were conditioned to artificial diet enriched with vanillin from …


Climate-Adapted Companion Cropping Increases Agricultural Productivity In East Africa, Charles A.O. Midega, Toby J.A. Bruce, John A. Pickett, Jimmy O. Pittchar, Alice Murage, Zeyaur R. Khan Aug 2015

Climate-Adapted Companion Cropping Increases Agricultural Productivity In East Africa, Charles A.O. Midega, Toby J.A. Bruce, John A. Pickett, Jimmy O. Pittchar, Alice Murage, Zeyaur R. Khan

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Production of cereals, the main staple and cash crops for millions of farmers in sub-Saharan Africa (SSA) is severely constrained by parasitic striga weed Striga hermonthica, stemborers and poor soil fertility. A companion cropping system known as 'push-pull' overcomes these constraints while providing additional soil fertility and forage grass benefits to smallholder farmers. To ensure the technology's long-term sustainability in view of the current and further potential aridification as a consequence of climate change, drought-tolerant crops, Brachiaria cv mulato (border crop) and greenleaf desmodium (intercrop), have been identified and incorporated into a 'climate-adapted push-pull'. The aims of the current study …


Evolution Of Spermophagus Seed Beetles (Coleoptera, Bruchinae, Amblycerini) Indicates Both Synchronous And Delayed Colonizations Of Host Plants, Gael J. Kergoat, Bruno P. Le Ru, Seyed E. Sadeghi, Midori Tuda, Chris A.M. Reid, Zoltán György, Gwenaëlle Genson, Cibele S. Ribeiro-Costa, Alex Delobel Aug 2015

Evolution Of Spermophagus Seed Beetles (Coleoptera, Bruchinae, Amblycerini) Indicates Both Synchronous And Delayed Colonizations Of Host Plants, Gael J. Kergoat, Bruno P. Le Ru, Seyed E. Sadeghi, Midori Tuda, Chris A.M. Reid, Zoltán György, Gwenaëlle Genson, Cibele S. Ribeiro-Costa, Alex Delobel

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Seed beetles are a group of specialized chrysomelid beetles, which are mostly associated with plants of the legume family (Fabaceae). In the legume-feeding species, a marked trend of phylogenetic conservatism of host use has been highlighted by several molecular phylogenetics studies. Yet, little is known about the evolutionary patterns of association of species feeding outside the legume family. Here, we investigate the evolution of host use in Spermophagus, a species-rich seed beetle genus that is specialized on two non-legume host-plant groups: morning glories (Convolvulaceae) and mallows (Malvaceae: Malvoideae). Spermophagus species are widespread in the Old World, especially in the Afrotropical, …


Unraveling Host-Vector-Arbovirus Interactions By Two-Gene High Resolution Melting Mosquito Bloodmeal Analysis In A Kenyan Wildlife-Livestock Interface, David Omondi, Daniel K. Masiga, Yvonne Ukamaka Ajamma, Burtram C. Fielding, Laban Njoroge, Jandouwe Villinger Jul 2015

Unraveling Host-Vector-Arbovirus Interactions By Two-Gene High Resolution Melting Mosquito Bloodmeal Analysis In A Kenyan Wildlife-Livestock Interface, David Omondi, Daniel K. Masiga, Yvonne Ukamaka Ajamma, Burtram C. Fielding, Laban Njoroge, Jandouwe Villinger

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The blood-feeding patterns of mosquitoes are directly linked to the spread of pathogens that they transmit. Efficient identification of arthropod vector bloodmeal hosts can identify the diversity of vertebrate species potentially involved in disease transmission cycles. While molecular bloodmeal analyses rely on sequencing of cytochrome b (cyt b) or cytochrome oxidase 1 gene PCR products, recently developed bloodmeal host identification based on high resolution melting (HRM) analyses of cyt b PCR products is more cost-effective. To resolve the diverse vertebrate hosts that mosquitoes may potentially feed on in sub-Saharan Africa, we utilized HRM profiles of both cyt b and 16S …


Genome-Wide Analysis Of Signatures Of Selection In Populations Of African Honey Bees (Apis Mellifera) Using New Web-Based Tools, Zachary L. Fuller, Elina L. Niño, Harland M. Patch, Oscar C. Bedoya-Reina, Tracey Baumgarten, Elliud Muli, Fiona Mumoki, Aakrosh Ratan, John Mcgraw, Maryann Frazier, Daniel Masiga, Stephen Schuster, Christina M. Grozinger, Webb Miller Jul 2015

Genome-Wide Analysis Of Signatures Of Selection In Populations Of African Honey Bees (Apis Mellifera) Using New Web-Based Tools, Zachary L. Fuller, Elina L. Niño, Harland M. Patch, Oscar C. Bedoya-Reina, Tracey Baumgarten, Elliud Muli, Fiona Mumoki, Aakrosh Ratan, John Mcgraw, Maryann Frazier, Daniel Masiga, Stephen Schuster, Christina M. Grozinger, Webb Miller

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Background: With the development of inexpensive, high-throughput sequencing technologies, it has become feasible to examine questions related to population genetics and molecular evolution of non-model species in their ecological contexts on a genome-wide scale. Here, we employed a newly developed suite of integrated, web-based programs to examine population dynamics and signatures of selection across the genome using several well-established tests, including F ST, pN/pS, and McDonald-Kreitman. We applied these techniques to study populations of honey bees (Apis mellifera) in East Africa. In Kenya, there are several described A. mellifera subspecies, which are thought to be localized to distinct ecological regions. …


Grower Adoption Of An Integrated Pest Management Package For Management Of Mango-Infesting Fruit Flies (Diptera: Tephritidae) In Embu, Kenya, J. K. Korir, H. D. Affognon, C. N. Ritho, W. S. Kingori, P. Irungu, S. A. Mohamed, S. Ekesi Jun 2015

Grower Adoption Of An Integrated Pest Management Package For Management Of Mango-Infesting Fruit Flies (Diptera: Tephritidae) In Embu, Kenya, J. K. Korir, H. D. Affognon, C. N. Ritho, W. S. Kingori, P. Irungu, S. A. Mohamed, S. Ekesi

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Mango (Mangifera indica L.) is a well-recognized fruit of economic and nutritional importance to smallholder farmers across Africa. Production and marketing of this fruit is, however, severely hampered by fruit fly infestation that is responsible for enormous fruit losses. In Kenya, control of this pest is primarily dependent on the use of chemical pesticides, a strategy that has been shown to be ineffective. Although the recently introduced fruit fly integrated pest management (IPM) package developed by the International Centre of Insect Physiology and Ecology (icipe) has proved to be effective against this pest, there is little demonstrable investigation on its …


Adoption Of Modern Beekeeping And Its Impact On Honey Production In The Former Mwingi District Of Kenya: Assessment Using Theory-Based Impact Evaluation Approach, Hippolyte D. Affognon, W. S. Kingori, A. I. Omondi, M. G. Diiro, B. W. Muriithi, S. Makau, S. K. Raina Jun 2015

Adoption Of Modern Beekeeping And Its Impact On Honey Production In The Former Mwingi District Of Kenya: Assessment Using Theory-Based Impact Evaluation Approach, Hippolyte D. Affognon, W. S. Kingori, A. I. Omondi, M. G. Diiro, B. W. Muriithi, S. Makau, S. K. Raina

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This study used the theory-based impact evaluation approach to understand as to how promotion of beekeeping by the Commercial Insects Programme (CIP) of the International Centre of Insect Physiology and Ecology (icipe) has had an impact on honey production in the former Mwingi District of Kenya (now merged into Kitui County). We examined the adoption of modern hives promoted by icipe and applied data envelopment analysis (DEA) to assess the technical efficiency (TE) of participants in CIP. CIP participants had an average TE of 0.56, which was significantly higher than that of non-participants in the former Kitui District (average TE …


Predicting The Impact Of Temperature Change On The Future Distribution Of Maize Stem Borers And Their Natural Enemies Along East African Mountain Gradients Using Phenology Models, Sizah Mwalusepo, Henri E.Z. Tonnang, Estomih S. Massawe, Gerphas O. Okuku, Nancy Khadioli, Tino Johansson, Paul André Calatayud, Bruno Pierre Le Ru Jun 2015

Predicting The Impact Of Temperature Change On The Future Distribution Of Maize Stem Borers And Their Natural Enemies Along East African Mountain Gradients Using Phenology Models, Sizah Mwalusepo, Henri E.Z. Tonnang, Estomih S. Massawe, Gerphas O. Okuku, Nancy Khadioli, Tino Johansson, Paul André Calatayud, Bruno Pierre Le Ru

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Lepidopteran stem borers are among the most important pests of maize in East Africa. The objective of the present study was to predict the impact of temperature change on the distribution and abundance of the crambid Chilo partellus, the noctuid Busseola fusca, and their larval parasitoids Cotesia flavipes and Cotesia sesamiae at local scale along Kilimanjaro and Taita Hills gradients in Tanzania and Kenya, respectively. Temperature-dependent phenology models of pests and parasitoids were used in a geographic information system for mapping. The three risk indices namely establishment, generation, and activity indices were computed using current temperature data record from local …


Mosquito Attraction: Crucial Role Of Carbon Dioxide In Formulation Of A Five-Component Blend Of Human-Derived Volatiles, Joop J.A. Van Loon, Renate C. Smallegange, Gabriella Bukovinszkiné-Kiss, Frans Jacobs, Marjolein De Rijk, Wolfgang R. Mukabana, Niels O. Verhulst, David J. Menger, Willem Takken Jun 2015

Mosquito Attraction: Crucial Role Of Carbon Dioxide In Formulation Of A Five-Component Blend Of Human-Derived Volatiles, Joop J.A. Van Loon, Renate C. Smallegange, Gabriella Bukovinszkiné-Kiss, Frans Jacobs, Marjolein De Rijk, Wolfgang R. Mukabana, Niels O. Verhulst, David J. Menger, Willem Takken

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Behavioral responses of the malaria mosquito Anopheles coluzzii (An. gambiae sensu stricto molecular ‘M form’) to an expanded blend of human-derived volatiles were assessed in a dual-port olfactometer. A previously documented attractive three-component blend consisting of NH3, (S)-lactic acid, and tetradecanoic acid served as the basis for expansion. Adding 4.5 % CO2 to the basic blend significantly enhanced its attractiveness. Expansion of the blend with four human-derived C4-volatiles was then assessed, both with and without CO2. Only when CO2 was offered simultaneously, did addition of a specific concentration of 3-methyl-1-butanol or 3-methyl-butanoic acid significantly enhance attraction. The functional group at …