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Articles 571 - 600 of 1129

Full-Text Articles in Entomology

Description Of New Ceratitis Species (Diptera: Tephritidae) From Africa, Or How Morphological And Dna Data Are Complementary In Discovering Unknown Species And Matching Sexes, Marc De Meyer, Maulid Mwatawala, Robert S. Copeland, Massimiliano Virgilio Sep 2016

Description Of New Ceratitis Species (Diptera: Tephritidae) From Africa, Or How Morphological And Dna Data Are Complementary In Discovering Unknown Species And Matching Sexes, Marc De Meyer, Maulid Mwatawala, Robert S. Copeland, Massimiliano Virgilio

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This paper describes five new Ceratitis species from the eastern and southern parts of the Afrotropical Region: C. (Pterandrus) quilicii De Meyer, Mwatawala & Virgilio sp. nov.; C. (Ceratalaspis) pallidula De Meyer, Mwatawala & Virgilio sp. nov.; C. (Ceratalaspis) taitaensis De Meyer & Copeland sp. nov.; C. (Ceratalaspis) sawahilensis De Meyer & Virgilio sp. nov.; and C. (Ceratalaspis) flavipennata De Meyer & Virgilio sp. nov. Their relationships with closely allied species within their respective subgenera are discussed where appropriate, and diagnostic characters are given. DNA barcodes are provided for all new species. In addition, the hitherto unknown male of C. …

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Developmental Biology And Demographic Parameters Of Antestia Bug Antestiopsis Thunbergii (Hemiptera: Pentatomidae), On Coffea Arabica (Rubiaceae) At Different Constant Temperatures, Abdelmutalab Gesmalla Ahmed, Lucy Kananu Murungi, Regis Babin Sep 2016

Developmental Biology And Demographic Parameters Of Antestia Bug Antestiopsis Thunbergii (Hemiptera: Pentatomidae), On Coffea Arabica (Rubiaceae) At Different Constant Temperatures, Abdelmutalab Gesmalla Ahmed, Lucy Kananu Murungi, Regis Babin

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The antestia bug Antestiopsis thunbergii (Gmelin 1790) is a major coffee pest in East Africa. Nymphs and adults feed on all vegetative and fruiting parts of the coffee tree leading to yield reduction and poor quality of coffee beans. Our study aimed to characterize the developmental biology and provide life table parameters for A. thunbergii reared in the laboratory. The biology of A. thunbergii was studied at four constant temperatures of 20, 25, 30 and 35°C with 80±5% RH and a photoperiod of L:D 12:12. Complete development of A. thunbergii from egg to adult occurred between 20 and 30°C, while …

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Chemical Parameters And Bacterial Communities Associated With Larval Habitats Of Anopheles, Culex And Aedes Mosquitoes (Diptera: Culicidae) In Western Kenya, Thomas Ogao Onchuru, Yvonne Ukamaka Ajamma, Marion Burugu, Martin Kaltenpoth, Daniel Masiga, Jandouwe Villinger Sep 2016

Chemical Parameters And Bacterial Communities Associated With Larval Habitats Of Anopheles, Culex And Aedes Mosquitoes (Diptera: Culicidae) In Western Kenya, Thomas Ogao Onchuru, Yvonne Ukamaka Ajamma, Marion Burugu, Martin Kaltenpoth, Daniel Masiga, Jandouwe Villinger

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Aquatic larval habitat conditions influence the development, fitness and vectorial capacity of mosquitoes. In turn, human activities can influence these conditions and shape mosquito vector distribution, thus affecting pathogen circulation and transmission. We measured environmental factors (chemical and microbial) in mosquito larval habitats and evaluated their potential as predictors for the occurrence of different mosquito species in an arbovirus and malaria endemic region of western Kenya.We found significantly greater proportions of Aedes aegypti mosquito larvae in ammonium and phosphate rich habitats, suggesting that fertilizer usage could potentially increase aquatic habitat suitability and Aedes vectored disease transmission. Anopheles gambiae larval habitats …

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Effects Of Entomopathogenic Fungus Metarhizium Anisopliae On Non-Target Ants Associated With Odontotermes Spp. (Isoptera: Termitidae) Termite Mounds In Kenya, E. A. Abonyo, N. K. Maniania, C. M. Warui, E. D. Kokwaro, T. M. Palmer, D. F. Doak, A. K. Brody Sep 2016

Effects Of Entomopathogenic Fungus Metarhizium Anisopliae On Non-Target Ants Associated With Odontotermes Spp. (Isoptera: Termitidae) Termite Mounds In Kenya, E. A. Abonyo, N. K. Maniania, C. M. Warui, E. D. Kokwaro, T. M. Palmer, D. F. Doak, A. K. Brody

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Termites are an important component of savannah ecosystems throughout Africa. Despite their importance in the ecosystem, they can be serious pests of structures, houses, rangelands, tropical forestry, and agriculture. For many decades, chemical insecticides have remained popular for termite management worldwide. However, with the growing environmental concerns over pesticides, biological control using entomopathogenic fungi such as Metarhizium anisopliae (Metschnikoff) Sorokin has become an often-considered alternative. Metarhizium anisopliae is an ubiquitous, naturally occurring pathogen, which has been reported infecting over 200 insect species; therefore, there is concern that use of M. anisopliae may affect non-target organisms. The effects of M. anisopliae …

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Farmers' Knowledge And Perception Of Grain Legume Pests And Their Management In The Eastern Province Of Kenya, Andnet Abtew, Saliou Niassy, Hippolyte Affognon, Sevgan Subramanian, Serge Kreiter, Giovanna Tropea Garzia, Thibaud Martin Sep 2016

Farmers' Knowledge And Perception Of Grain Legume Pests And Their Management In The Eastern Province Of Kenya, Andnet Abtew, Saliou Niassy, Hippolyte Affognon, Sevgan Subramanian, Serge Kreiter, Giovanna Tropea Garzia, Thibaud Martin

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Grain legumes play an important role in community livelihood and in the national economy in Kenya. Unfortunately, in many African countries, production doesn't satisfy the demand in grains due to various constrains. Understanding farmers practices and behavior in the management of grain legume pests is a crucial step in the development of sustainable management strategies. A total of 216 farmers were surveyed in eight districts of eastern Kenya to evaluate farmers' knowledge and perceptions of grain legume pests; to examine current pest management practices, and to identify other production constraints. Grain legumes are grown by a wide age-group of farmers, …

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First Record Of Tuta Absoluta (Lepidoptera: Gelechiidae) In Uganda, Venansio Tumuhaise, Fathiya M. Khamis, Ambrose Agona, Godfrey Sseruwu, Samira A. Mohamed Sep 2016

First Record Of Tuta Absoluta (Lepidoptera: Gelechiidae) In Uganda, Venansio Tumuhaise, Fathiya M. Khamis, Ambrose Agona, Godfrey Sseruwu, Samira A. Mohamed

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An invasive pest, Tuta absoluta (Meyrick) has been observed infesting tomato Solanum lycopersicum L. (Solanales: Solanaceae) in Central Uganda. The affected farmer made the report, and upon visiting the farm, we observed tiny larvae, green to cream in colour with a black head feeding on tomato leaves, fruit, as well as tender stems. Also, we saw several tiny moths resting on screen house linings. Delta traps baited with TUA-Optima® (Russell IPM) were set in and around the affected farm. Dozens of moths were attracted and trapped on the sticky surface of the traps. The team tentatively identified the insect as …

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Assessing The Long-Term Welfare Effects Of The Biological Control Of Cereal Stemborer Pests In East And Southern Africa: Evidence From Kenya, Mozambique And Zambia, Soul Kifouly G. Midingoyi, Hippolyte D. Affognon, Ibrahim Macharia, Georges Ong'amo, Esther Abonyo, Gerphas Ogola, Hugo De Groote, Bruno Leru Aug 2016

Assessing The Long-Term Welfare Effects Of The Biological Control Of Cereal Stemborer Pests In East And Southern Africa: Evidence From Kenya, Mozambique And Zambia, Soul Kifouly G. Midingoyi, Hippolyte D. Affognon, Ibrahim Macharia, Georges Ong'amo, Esther Abonyo, Gerphas Ogola, Hugo De Groote, Bruno Leru

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The International Centre of Insect Physiology and Ecology (icipe), undertook a biological control (BC) programme for control of stemborers from 1993 to 2008, to reduce cereal yield losses due to stemborer attack in East and Southern Africa. The programme released four biological control agents-the larval parasitoids Cotesia flavipes and Cotesia sesamiae, the egg parasitoid Telenomus isis and the pupal parasitoid Xanthopimpla stemmator-to control the economically important stemborer pests Busseola fusca, Chilo partellus and Sesamia calamistis. Two of the natural enemies that were released got established and spread to many localities in the region. This study adopted the economic surplus model …

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Prospects For Repellent In Pest Control: Current Developments And Future Challenges, Emilie Deletre, Bertrand Schatz, Denis Bourguet, Fabrice Chandre, Livy Williams, Alain Ratnadass, Thibaud Martin Aug 2016

Prospects For Repellent In Pest Control: Current Developments And Future Challenges, Emilie Deletre, Bertrand Schatz, Denis Bourguet, Fabrice Chandre, Livy Williams, Alain Ratnadass, Thibaud Martin

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The overall interest in environmentally safe pest control methods and the rise of insecticide resistance in pest populations have prompted medical and agricultural entomology research on insect repellents in recent years. However, conducting research on repellent is challenging for several reasons: (1) the different repellent phenomena are not well defined; (2) it is difficult to test for and quantify repellent; (3) the physiological mechanisms are poorly known; (4) the field efficacy appears to be highly variable. Here, we identified five different types of repellent: expellency, irritancy, deterrency, odor masking and visual masking, and described behavioral bioassays to differentiate between them. …

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Storage Of Pigeonpea Grain (Cajanus Cajan (L.) Millsp.) In Hermetic Triple-Layer Bags Prevents Losses Caused By Callosobruchus Maculatus (F.) (Coleoptera: Bruchidae), H. D. Affognon, A. W. Njoroge, C. M. Mutungi, J. Manono, D. Baributsa, L. L. Murdock Jul 2016

Storage Of Pigeonpea Grain (Cajanus Cajan (L.) Millsp.) In Hermetic Triple-Layer Bags Prevents Losses Caused By Callosobruchus Maculatus (F.) (Coleoptera: Bruchidae), H. D. Affognon, A. W. Njoroge, C. M. Mutungi, J. Manono, D. Baributsa, L. L. Murdock

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Dry pigeonpea grain is susceptible to infestation from the cowpea bruchid during storage. This study investigated the potential of Purdue Improved Cowpea Storage (PICS™) and polypropylene (PP) bags for reducing insect infestation under ambient laboratory conditions for six months. Some of the bags were artificially infested with cowpea bruchid Callosobruchus maculatus (PICS1, PP1) while others were not (PICS0, PP0). In an additional trial, PP bags containing pigeonpeas were treated with Actellic Super® dust before being artificially infested (PP1Ac). Moisture content, number of live C. maculatus adults, grain damage, and weight loss were determined at monthly intervals. Temperature and relative humidity …

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An Indirect Defence Trait Mediated Through Egg-Induced Maize Volatiles From Neighbouring Plants, Daniel M. Mutyambai, Toby J.A. Bruce, Johnnie Van Den Berg, Charles A.O. Midega, John A. Pickett, Zeyaur R. Khan Jul 2016

An Indirect Defence Trait Mediated Through Egg-Induced Maize Volatiles From Neighbouring Plants, Daniel M. Mutyambai, Toby J.A. Bruce, Johnnie Van Den Berg, Charles A.O. Midega, John A. Pickett, Zeyaur R. Khan

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Attack of plants by herbivorous arthropods may result in considerable changes to the plant's chemical phenotype with respect to emission of herbivore-induced plant volatiles (HIPVs). These HIPVs have been shown to act as repellents to the attacking insects as well as attractants for the insects antagonistic to these herbivores. Plants can also respond to HIPV signals from other plants that warn them of impending attack. Recent investigations have shown that certain maize varieties are able to emit volatiles following stemborer egg deposition. These volatiles attract the herbivore's parasitoids and directly deter further oviposition. However, it was not known whether these …

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Redescription Of Helorus Ruficornis Förster (Hymenoptera: Heloridae), With A New Synonymy And New Afrotropical Specimen Records, Matthew L. Buffington, Robert S. Copeland Jul 2016

Redescription Of Helorus Ruficornis Förster (Hymenoptera: Heloridae), With A New Synonymy And New Afrotropical Specimen Records, Matthew L. Buffington, Robert S. Copeland

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Research up to this point has recorded two species of Afrotropical Heloridae: Helorus ruficornis Förster and H. elgoni Risbec. After examining recently collected specimens from Kenya and Burundi, we have been able to better understand intraspecific variation within Helorus ruficornis, and as a result, we hereby synonymize H. elgoni with H. ruficornis, redescribe H. ruficornis, and illustrate the holotype of H. elgoni for the first time. We also discuss new collection records for Afrotropical Heloridae, as well as possible habitat preferences.

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Push-Pull: Chemical Ecology-Based Integrated Pest Management Technology, Zeyaur Khan, Charles A.O. Midega, Antony Hooper, John Pickett Jul 2016

Push-Pull: Chemical Ecology-Based Integrated Pest Management Technology, Zeyaur Khan, Charles A.O. Midega, Antony Hooper, John Pickett

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Lepidopterous stemborers, and parasitic striga weeds belonging to the family Orobanchaceae, attack cereal crops in sub-Saharan Africa causing severe yield losses. The smallholder farmers are resource constrained and unable to afford expensive chemicals for crop protection. The push–pull technology, a chemical ecology- based cropping system, is developed for integrated pest and weed management in cereal–livestock farming systems. Appropriate plants were selected that naturally emit signaling chemicals (semiochemicals). Plants highly attractive for stemborer egg laying were selected and employed as trap crops (pull), to draw pests away from the main crop. Plants that repelled stemborer females were selected as intercrops (push). …

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Vector Competence Of Aedes Aegypti In Transmitting Chikungunya Virus: Effects And Implications Of Extrinsic Incubation Temperature On Dissemination And Infection Rates, Sophiah Mbaika, Joel Lutomiah, Edith Chepkorir, Francis Mulwa, Christopher Khayeka-Wandabwa, Caroline Tigoi, Elijah Oyoo-Okoth, James Mutisya, Zipporah Ng'ang'a, Rosemary Sang Jun 2016

Vector Competence Of Aedes Aegypti In Transmitting Chikungunya Virus: Effects And Implications Of Extrinsic Incubation Temperature On Dissemination And Infection Rates, Sophiah Mbaika, Joel Lutomiah, Edith Chepkorir, Francis Mulwa, Christopher Khayeka-Wandabwa, Caroline Tigoi, Elijah Oyoo-Okoth, James Mutisya, Zipporah Ng'ang'a, Rosemary Sang

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Background: Aedes aegypti is a competent arthropod vector of chikungunya virus (CHIKV). The rate at which the virus disseminate in the vector is limited by temperature of their environment which can be an important determinant of geographical and seasonal limits to transmission by the arthropods in the tropics. This study investigated the vector competence of Ae. aegypti for CHIKV at ambient temperature of 32 and 26 °C (Coastal and Western Kenya respectively) reared at Extrinsic Incubation Temperature (EIT) of 32 and 26 °C that resembles those in the two regions. Methods: Ae. aegypti eggs were collected from coastal and Western …

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Low Permeability Triple-Layer Plastic Bags Prevent Losses Of Maize Caused By Insects In Rural On-Farm Stores, Jeremiah Ng’Ang’A, Christopher Mutungi, Samuel M. Imathiu, Hippolyte Affognon Jun 2016

Low Permeability Triple-Layer Plastic Bags Prevent Losses Of Maize Caused By Insects In Rural On-Farm Stores, Jeremiah Ng’Ang’A, Christopher Mutungi, Samuel M. Imathiu, Hippolyte Affognon

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Participatory on-farm trials were conducted to assess effectiveness of Purdue Improved Crop Storage (PICS™) bags for storage of maize in small-scale farmers’ stores in rural villages in eastern Kenya. A PICS bag is a three-layered hermetic bag-system that forms a barrier against the influx of oxygen and the escape of carbon dioxide. Jute, woven polypropylene or PICS bags were filled with shelled maize grain, purchased from the participating farmers, and the three sets of bags kept in the farmers’ own stores for 35 weeks. Oxygen and carbon dioxide levels in the PICS bags were monitored, as well as the temperature …

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Molecular Identification Of Different Trypanosome Species And Subspecies In Tsetse Flies Of Northern Nigeria, Clement Isaac, Marc Ciosi, Alana Hamilton, Kathleen Maria Scullion, Peter Dede, Igho Benjamin Igbinosa, Oyebiguwa Patrick Goddey Nmorsi, Dan Masiga, C. Michael R. Turner May 2016

Molecular Identification Of Different Trypanosome Species And Subspecies In Tsetse Flies Of Northern Nigeria, Clement Isaac, Marc Ciosi, Alana Hamilton, Kathleen Maria Scullion, Peter Dede, Igho Benjamin Igbinosa, Oyebiguwa Patrick Goddey Nmorsi, Dan Masiga, C. Michael R. Turner

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Background: Animal African Trypanosomiasis (AAT) is caused by several species of trypanosomes including Trypanosoma congolense, T. vivax, T. godfreyi, T. simiae and T. brucei. Two of the subspecies of T. brucei also cause Human African Trypanosomiasis. Although some of them can be mechanically transmitted by biting flies; these trypanosomes are all transmitted by tsetse flies which are the cyclical vectors of Trypanosoma congolense, T. godfreyi, T. simiae and T. brucei. We present here the first report assessing the prevalence of trypanosomes in tsetse flies in Nigeria using molecular tools. Methods: 488 tsetse flies of three species, Glossina palpalis palpalis, G. …

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Can Climate-Driven Change Influence Silicon Assimilation By Cereals And Hence The Distribution Of Lepidopteran Stem Borers In East Africa?, P. A. Calatayud, E. Njuguna, S. Mwalusepo, M. Gathara, G. Okuku, A. Kibe, B. Musyoka, D. Williamson, G. Ong'amo, G. Juma, T. Johansson, S. Subramanian, E. Gatebe, B. Le Ru May 2016

Can Climate-Driven Change Influence Silicon Assimilation By Cereals And Hence The Distribution Of Lepidopteran Stem Borers In East Africa?, P. A. Calatayud, E. Njuguna, S. Mwalusepo, M. Gathara, G. Okuku, A. Kibe, B. Musyoka, D. Williamson, G. Ong'amo, G. Juma, T. Johansson, S. Subramanian, E. Gatebe, B. Le Ru

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In East Africa, lepidopteran stemborers such as Chilo partellus and Busseola fusca are major constraints to production of maize, which is the main staple food crop in the region. Cereals depend on silicon (Si)-based defences to fight off herbivores. Using altitudinal ranges in the East African highlands as ecological surrogates for inferring climate change, it was shown that Si concentrations in soil and maize decreased with altitude. This was attributed, in part, to low temperatures at high altitudes, which negatively affected Si assimilation by maize. Experiments showed that B. fusca was more susceptible to Si than C. partellus. Hence the …

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Gravid Anopheles Gambiae Sensu Stricto Avoid Ovipositing In Bermuda Grass Hay Infusion And It's Volatiles In Two Choice Egg-Count Bioassays, Lynda K. Eneh, Michael N. Okal, Anna Karin Borg-Karlson, Ulrike Fillinger, Jenny M. Lindh May 2016

Gravid Anopheles Gambiae Sensu Stricto Avoid Ovipositing In Bermuda Grass Hay Infusion And It's Volatiles In Two Choice Egg-Count Bioassays, Lynda K. Eneh, Michael N. Okal, Anna Karin Borg-Karlson, Ulrike Fillinger, Jenny M. Lindh

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Background: A number of mosquito species in the Culex and Aedes genera prefer to lay eggs in Bermuda grass (Cynodon dactylon) hay infusions compared to water alone. These mosquitoes are attracted to volatile compounds from the hay infusions making the infusions effective baits in gravid traps used for monitoring vectors of arboviral and filarial pathogens. Since Bermuda grass is abundant and widespread, it is plausible to explore infusions made from it as a potential low cost bait for outdoor monitoring of the elusive malaria vector Anopheles gambiae s.s. Methods: This study investigated preferential egg laying of individual An. gambiae s.s. …

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Influence Of Host-Plant Surface Chemicals On The Oviposition Of The Cereal Stemborer Busseola Fusca, Gerald Juma, Gilles Clément, Peter Ahuya, Ahmed Hassanali, Sylvie Derridj, Cyrile Gaertner, Romain Linard, Bruno Le Ru, Brigitte Frérot, Paul André Calatayud May 2016

Influence Of Host-Plant Surface Chemicals On The Oviposition Of The Cereal Stemborer Busseola Fusca, Gerald Juma, Gilles Clément, Peter Ahuya, Ahmed Hassanali, Sylvie Derridj, Cyrile Gaertner, Romain Linard, Bruno Le Ru, Brigitte Frérot, Paul André Calatayud

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The chemical composition of plant surfaces plays a role in selection of host plants by herbivorous insects. Once the insect reaches the plant, these cues determine host acceptance. Laboratory studies have shown that the stem borer Busseola fusca (Lepidoptera: Noctuidae), an important pest of sorghum and maize in sub-Saharan Africa, is able to differentiate between host and non-host plant species. However, no information is available on the cues used by this insect to seek and accept the host plant. Thus, the role of surface phytochemical stimuli on host selection and oviposition by B. fusca was studied in the laboratory using …

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Uncertainty And Sensitivity Analysis Of A Rift Valley Fever Model, S. A. Pedro, H. E.Z. Tonnang, S. Abelman Apr 2016

Uncertainty And Sensitivity Analysis Of A Rift Valley Fever Model, S. A. Pedro, H. E.Z. Tonnang, S. Abelman

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Rift Valley fever (RVF) is a vector-borne viral disease with pronounced health and economic impacts on domestic animals and humans in much of sub-Saharan Africa. Using techniques of uncertainty and sensitivity analysis (both local derivatives and sampling-based methods) of a mathematical model for RVF transmission in livestock by two population of mosquitoes (Aedes and Culex). We systematically investigate the relative importance of each model parameter for both disease epidemic and endemic activities. The relationship between vertical transmission and basic reproduction number reveals that during epidemic activities vertical transmission accelerates the course of the outbreak as it increases the size of …

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Chemical Composition Of Cupressus Lusitanica And Eucalyptus Saligna Leaf Essential Oils And Bioactivity Against Major Insect Pests Of Stored Food Grains, Philip K. Bett, Arop L. Deng, Joshua O. Ogendo, Samuel T. Kariuki, Maud Kamatenesi-Mugisha, Joel M. Mihale, Baldwyn Torto Apr 2016

Chemical Composition Of Cupressus Lusitanica And Eucalyptus Saligna Leaf Essential Oils And Bioactivity Against Major Insect Pests Of Stored Food Grains, Philip K. Bett, Arop L. Deng, Joshua O. Ogendo, Samuel T. Kariuki, Maud Kamatenesi-Mugisha, Joel M. Mihale, Baldwyn Torto

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The leaf essential oils from Cupressus lusitanica, Miller and Eucalyptus saligna, Smith obtained by hydrodistillation were analyzed by GC/MS and also screened for their insecticidal and repellent effects against adult Tribolium castaneum, Acanthoscelides obtectus, Sitotroga cerealella and Sitophilus zeamais. The C. lusitanica oil contained mainly umbellulone (18.38%) and α-pinene (9.97%) whereas the E. saligna oil was dominated by α-pinene (24.40%) and 1,8-cineole (24.26%). Bioassays showed that of the four insect species tested, A. obtectus and S. cerealella were the most susceptible to the oils, with LC50 values of 0.05-0.11% v/w in contact toxicity and 4.07-7.02μl/L air in space fumigation. Except …

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Impact Assessment Of Integrated Pest Management (Ipm) Strategy For Suppression Of Mango-Infesting Fruit Flies In Kenya, Beatrice W. Muriithi, Hippolyte D. Affognon, Gracious M. Diiro, Sarah W. Kingori, Chrysantus M. Tanga, Peterson W. Nderitu, Samira A. Mohamed, Sunday Ekesi Mar 2016

Impact Assessment Of Integrated Pest Management (Ipm) Strategy For Suppression Of Mango-Infesting Fruit Flies In Kenya, Beatrice W. Muriithi, Hippolyte D. Affognon, Gracious M. Diiro, Sarah W. Kingori, Chrysantus M. Tanga, Peterson W. Nderitu, Samira A. Mohamed, Sunday Ekesi

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We utilized two waves of data collected from a sample of mango farmers in Meru County in Kenya to evaluate the impact of Integrated Pest Management (IPM) strategy for controlling fruit flies in mango-production. We specifically explored the effect of five IPM practices including parasitoids (p) and Metarhizium anisopliae-based biopesticides (biop), orchard sanitation (os), spot spray of food bait (fb) and male annihilation technique (mat) on three outcome indicators: farmer pesticide expenditure, farm-level mango fruit yield losses and profit. We fitted difference-in-difference and household fixed effects regression models that account for unobserved heterogeneity across households. Our estimates differentiated the impact …

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Visual, Vibratory, And Olfactory Cues Affect Interactions Between The Red Spider Mite Tetranychus Evansi And Its Predator Phytoseiulus Longipes, Ginette Yèyinou Azandémè-Hounmalon, Baldwyn Torto, Komi Kouma Mokpokpo Fiaboe, Sevgan Subramanian, Serge Kreiter, Thibaud Martin Mar 2016

Visual, Vibratory, And Olfactory Cues Affect Interactions Between The Red Spider Mite Tetranychus Evansi And Its Predator Phytoseiulus Longipes, Ginette Yèyinou Azandémè-Hounmalon, Baldwyn Torto, Komi Kouma Mokpokpo Fiaboe, Sevgan Subramanian, Serge Kreiter, Thibaud Martin

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Phytoseiulus longipes Evans (Mesostigmata: Phytoseiidae) is an exotic predator widely used in biological control programs for the red spider mite Tetranychus evansi Baker & Pritchard (Acari: Tetranychidae) in East Africa. However, little is known about the cues mediating this prey/predator interaction. Here, we performed behavioral assays to test the involvement of visual, vibratory, and olfactory cues using a combination of dead/living insects enclosed in either perforated or non-perforated transparent/opaque capsules. We monitored insect responses with a video tracking system and analyzed the data with Ethovision software. Our results showed avoidance behavior of T. evansi in the presence of live P. …

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Genome-Wide Comparative Analysis Of Chemosensory Gene Families In Five Tsetse Fly Species, Rosaline Macharia, Paul Mireji, Edwin Murungi, Grace Murilla, Alan Christoffels, Serap Aksoy, Daniel Masiga Feb 2016

Genome-Wide Comparative Analysis Of Chemosensory Gene Families In Five Tsetse Fly Species, Rosaline Macharia, Paul Mireji, Edwin Murungi, Grace Murilla, Alan Christoffels, Serap Aksoy, Daniel Masiga

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For decades, odour-baited traps have been used for control of tsetse flies (Diptera; Glossinidae), vectors of African trypanosomes. However, differential responses to known attractants have been reported in different Glossina species, hindering establishment of a universal vector control tool. Availability of full genome sequences of five Glossina species offers an opportunity to compare their chemosensory repertoire and enhance our understanding of their biology in relation to chemosensation. Here, we identified and annotated the major chemosensory gene families in Glossina. We identified a total of 118, 115, 124, and 123 chemosensory genes in Glossina austeni, G. brevipalpis, G. f. fuscipes, G. …

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Host Stage Preference And Performance Of The Aphid Parasitoid Diaeretiella Rapae (Hymenoptera: Braconidae) On Brevicoryne Brassicae And Lipaphis Pseudobrassicae (Hemiptera: Aphididae), Sylvia W.R. Khakasa, Samira A. Mohamed, Zipporah O. Lagat, Fathiya M. Khamis, Chrysantus M. Tanga Feb 2016

Host Stage Preference And Performance Of The Aphid Parasitoid Diaeretiella Rapae (Hymenoptera: Braconidae) On Brevicoryne Brassicae And Lipaphis Pseudobrassicae (Hemiptera: Aphididae), Sylvia W.R. Khakasa, Samira A. Mohamed, Zipporah O. Lagat, Fathiya M. Khamis, Chrysantus M. Tanga

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We assessed the host stage preference and performance of the aphid parasitoid Diaeretiella rapae (McIntosh) on two aphid species, Brevicoryne brassicae (L.) and Lipaphis pseudobrassicae (Davis). Although the parasitoid parasitized all nymphal instars of both aphid species, it showed a higher oviposition preference for 2nd (48.2 ± 7.74%) and 3rd (41.0 ± 7.82%) instars of L. pseudobrassicae, and for the 3rd (40.0 ± 4.59%) instar of B. brassicae. Across the host species, there was no significant difference in parasitoid preference between the 3rd and 4th instars, while 1st and 2nd instars of L. pseudobrassicae were significantly more preferred than their …

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Identification Of Aphid (Hemiptera: Aphididae) Species Of Economic Importance In Kenya Using Dna Barcodes And Pcr-Rflp-Based Approach, G. Kinyanjui, F. M. Khamis, S. Mohamed, L. O. Ombura, M. Warigia, S. Ekesi Feb 2016

Identification Of Aphid (Hemiptera: Aphididae) Species Of Economic Importance In Kenya Using Dna Barcodes And Pcr-Rflp-Based Approach, G. Kinyanjui, F. M. Khamis, S. Mohamed, L. O. Ombura, M. Warigia, S. Ekesi

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Aphids are among pests of economic importance throughout the world. Together with transmitting plant viruses, aphids are capable of inflicting severe crop production losses. They also excrete honeydew that favours the growth of sooty mold which reduces the quality of vegetables and fruits and hence their market values. Rapid and accurate identification of aphids to the species level is a critical component in effective pest management and plant quarantine systems. Even though morphological taxonomy has made a tremendous impact on species-level identifications, polymorphism, morphological plasticity and immature stages are among the many challenges to accurate identification. In addition, their small …

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Developing And Deploying Insect Resistant Maize Varieties To Reduce Pre-And Post-Harvest Food Losses In Africa, Tadele Tefera, Stephen Mugo, Yoseph Beyene Feb 2016

Developing And Deploying Insect Resistant Maize Varieties To Reduce Pre-And Post-Harvest Food Losses In Africa, Tadele Tefera, Stephen Mugo, Yoseph Beyene

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Maize grain yield in Africa is low, 1.5 t ha−1 compared to the global average of 4.9 t ha−1. Maize production in Africa is constrained by various abiotic (drought, soil fertility) and biotic factors (insect pests, weeds and diseases). Stem borers and postharvest insect pests play considerable roles in reducing maize yield through damaging the leaves, stems, ears, and kernels. Stem borers can cause 10–15 % yield losses while the postharvest insect pests, particularly, the larger grain borer and maize weevil, can cause 14–36 % grain losses. The use of chemical insecticides has been recommended; however, in addition to health …

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Bivalent Carbamates As Novel Control Agents Of The Malaria Mosquito, Anopheles Gambiae, James M. Mutunga, Qiao Hong Chen, Dawn M. Wong, Polo C.H. Lam, Jianyong Li, Maxim M. Totrov, Aaron D. Gross, Paul R. Carlier, Jeffrey R. Bloomquist Jan 2016

Bivalent Carbamates As Novel Control Agents Of The Malaria Mosquito, Anopheles Gambiae, James M. Mutunga, Qiao Hong Chen, Dawn M. Wong, Polo C.H. Lam, Jianyong Li, Maxim M. Totrov, Aaron D. Gross, Paul R. Carlier, Jeffrey R. Bloomquist

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Widespread pyrethroid resistance has caused an urgent need to develop new insecticides for control of the malaria mosquito, Anopheles gambiae. Insecticide discovery efforts were directed towards the construction of bivalent inhibitors that occupy both the peripheral and catalytic sites of the mosquito acetylcholinesterase (AChE). It was hypothesized that this approach would yield a selective, high potency inhibitor that would also circumvent known catalytic site mutations (e.g. G119S) causing target site resistance. Accordingly, a series of bivalent phthalimide-pyrazole carbamates were prepared having an alkyl chain linker of varying length, along with other modifications. The most active compound was (1-(3-(1,3-dioxoisoindolin-2-yl)propyl)-1H-pyrazol-4-yl methylcarbamate, 8a), …

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Rapid And High Throughput Molecular Identification Of Diverse Mosquito Species By High Resolution Melting Analysis., Yvonne Ukamaka Ajamma, Enock Mararo, David Omondi, Thomas Onchuru, Anne W.T. Muigai, Daniel Masiga, Jandouwe Villinger Jan 2016

Rapid And High Throughput Molecular Identification Of Diverse Mosquito Species By High Resolution Melting Analysis., Yvonne Ukamaka Ajamma, Enock Mararo, David Omondi, Thomas Onchuru, Anne W.T. Muigai, Daniel Masiga, Jandouwe Villinger

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Mosquitoes are a diverse group of invertebrates, with members that are among the most important vectors of diseases. The correct identification of mosquitoes is paramount to the control of the diseases that they transmit. However, morphological techniques depend on the quality of the specimen and often unavailable taxonomic expertise, which may still not be able to distinguish mosquitoes among species complexes (sibling and cryptic species). High resolution melting (HRM) analyses, a closed-tube, post-polymerase chain reaction (PCR) method used to identify variations in nucleic acid sequences, has been used to differentiate species within the Anopheles gambiae and Culex pipiens complexes. We …

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Fruit Fly Species Composition, Distribution And Host Plants With Emphasis On Mango- Infesting Species, Ivan Rwomushana, Chrysantus M. Tanga Jan 2016

Fruit Fly Species Composition, Distribution And Host Plants With Emphasis On Mango- Infesting Species, Ivan Rwomushana, Chrysantus M. Tanga

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Mango is the most widely cultivated fruit tree in tropical and sub-tropical Africa. However, the sustainability of this lucrative business is threatened by infestations of fruit flies (Diptera: Tephritidae) that annually inflict heavy economic losses on the industry. The nutritional quality of different fruit species can influence the survival and fecundity of adult fruit flies. This host-insect interaction determines the species composition, distribution and abundance of the major frugivorous tephritids. The economic impact of fruit fly pest species includes direct yield losses and the loss of export markets due to quarantine restrictions implemented to prevent the entry and establishment of …

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Role Of Microsatellite Markers In Molecular Population Genetics Of Fruit Flies With Emphasis On The Bactrocera Dorsalis Invasion Of Africa, Fathiya M. Khamis, Anna R. Malacrida Jan 2016

Role Of Microsatellite Markers In Molecular Population Genetics Of Fruit Flies With Emphasis On The Bactrocera Dorsalis Invasion Of Africa, Fathiya M. Khamis, Anna R. Malacrida

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Microsatellites, also referred to as short tandem repeats (STRs) or simple sequence repeats (SSRs) are short sequences of tandem repeats of 1-6 bp in length in clusters of less than 150 bp flanked by sections of non-repetitive unique sequences that are scattered throughout the nuclear genome. These markers are co-dominant and hypervariable, revealing many alleles per locus; they are inherited in Mendelian fashion making them useful for detecting genetic variability within species. Once isolated and characterized, microsatellites can also be used in closely related taxa. Microsatellites can be amplified, even from highly degraded DNA, and are very simple to score. …

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