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Articles 151 - 157 of 157

Full-Text Articles in Biology

The N-Terminal Prodomain Of Sv23 Is Essential For The Assembly Of A Functional Vitelline Membrane Network In Drosophila, Anita L. Manogaran, Gail L. Waring Jun 2004

The N-Terminal Prodomain Of Sv23 Is Essential For The Assembly Of A Functional Vitelline Membrane Network In Drosophila, Anita L. Manogaran, Gail L. Waring

Biological Sciences Faculty Research and Publications

The vitelline membrane is a specialized extracellular matrix that surrounds and protects the oocyte. Recent studies indicate that it also serves as a storage site for embryonic pattern determinants. sV23, a major vitelline membrane protein, is essential for the morphogenesis of the vitelline membrane as sV23 protein null mutants lay flaccid, infertile eggs. By analyzing a series of sV23 mutant transgenes in the sV23 protein null genetic background, we have shown that sV23 is secreted as a proprotein in functional excess and that C- and N-terminal prodomains are removed successively, following its deposition in the extracellular space. Although a target …

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The Cohesion Protein Ord Is Required For Homologue Bias During Meiotic Recombination, Hayley A. Webber, Louisa Howard, Sharon E. Bickel Mar 2004

The Cohesion Protein Ord Is Required For Homologue Bias During Meiotic Recombination, Hayley A. Webber, Louisa Howard, Sharon E. Bickel

Dartmouth Scholarship

During meiosis, sister chromatid cohesion is required for normal levels of homologous recombination, although how cohesion regulates exchange is not understood. Null mutations in orientation disruptor (ord) ablate arm and centromeric cohesion during Drosophila meiosis and severely reduce homologous crossovers in mutant oocytes. We show that ORD protein localizes along oocyte chromosomes during the stages in which recombination occurs. Although synaptonemal complex (SC) components initially associate with synapsed homologues in ord mutants, their localization is severely disrupted during pachytene progression, and normal tripartite SC is not visible by electron microscopy. In ord germaria, meiotic double strand breaks appear …

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Fc177, A Minor Dec-1 Proprotein, Is Necessary To Prevent Ectopic Aggregation Of The Endochorion During Eggshell Assembly In Drosophila, Debra Kay Mauzy-Melitz, Gail L. Waring Mar 2003

Fc177, A Minor Dec-1 Proprotein, Is Necessary To Prevent Ectopic Aggregation Of The Endochorion During Eggshell Assembly In Drosophila, Debra Kay Mauzy-Melitz, Gail L. Waring

Biological Sciences Faculty Research and Publications

The Drosophila eggshell is a highly specialized extracellular matrix that forms between the oocyte and the surrounding epithelial follicle cells during late oogenesis. The dec-1 gene, which is required for proper eggshell assembly, produces three proproteins that are cleaved within the vitelline membrane layer to multiple derivatives. The different spatial distributions of the cleaved derivatives suggest that they play distinct roles in eggshell assembly. Using extant dec-1 mutations in conjunction with genetically engineered dec-1 transgenes, we show that, although all three dec-1 proproteins, fc106, fc125, and fc177, are required for female fertility, gross morphological abnormalities in the eggshell are observed …

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Anti-Genotoxic Effect Of Ascorbic Acid On Mutagenic Dose Of Three Alkylating Agents, Bülent Kaya Jan 2003

Anti-Genotoxic Effect Of Ascorbic Acid On Mutagenic Dose Of Three Alkylating Agents, Bülent Kaya

Turkish Journal of Biology

The antimutagenic effect of ascorbic acid (vitamin C) was investigated by using the Drosophila wing spot test. In this assay, 3-day-old transheterozygous larvae for the multiple wing hair (mwh, 3-0.3) and flare (flr, 3-38.8) genes were treated with 3 direct acting mutagens: ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS) and N-nitroso N-ethylurea (ENU). The results obtained from 3 reference mutagens were clearly genotoxic in the Drosophila wing somatic mutation and recombination test. The calculated concentration of ascorbic acid as recommended dietary allowance (RDA) did not induce the frequency of the mutant clones recorded. When co-treatment experiments with ascorbic acid were carried …

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Lack Of Minority Advantage In Drosophilamelanogaster Mutants, Şükran Çakir, Aykut Kence Jan 1999

Lack Of Minority Advantage In Drosophilamelanogaster Mutants, Şükran Çakir, Aykut Kence

Turkish Journal of Biology

The objective of this study was to test the generality of frequency-dependent fitness in the mating behavior of Drosophila, called the minority effect and to investigate the effect of artificial olfactory cues and marking the flies by wing cillipng on the frequency-dependent mate choice. In the mating experiments using wild type (oregon) and mutant (white-eyed, sepia, ebony) strains of Drosophila melanogaster, the mating success of mutant males did not increase as their frequency decreased. In addition, the olfactory cue hypothesis was not supported by the results of the experiments with artificial scent. The results of this study did not support …

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The Functional Organization Of The Vestigial Locus In Drosophila Melanogaster, Jim A. Williams, Audrey L. Atkin, John B. Bell Jan 1990

The Functional Organization Of The Vestigial Locus In Drosophila Melanogaster, Jim A. Williams, Audrey L. Atkin, John B. Bell

School of Biological Sciences: Faculty Publications

Vestigial mutants are associated with imaginal disc cell death which results in the deletion of adult wing and haltere structures. The vestigial locus has previously been cloned, and mutational lesions associated with a number of vg alleles were mapped within a 19 kb DNA region defined as essential for vg function. Herein we report the identification and characterization of a developmentally regu-lated 3.8 kb vg transcript which is spliced from exons distributed throughout the essential interval defined above. All the characterized classical alleles have predictable effects on this transcription unit, and the severity of this effect is directly proportional to …

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The Effect Of Temperature On The Life Cycle Of Drosophila Nebulosa, Scott S. Nagatani Jan 1978

The Effect Of Temperature On The Life Cycle Of Drosophila Nebulosa, Scott S. Nagatani

University of the Pacific Theses and Dissertations

It is the purpose of this research to study the effect of temperature on the life cycle of D. nebulosa. D. nebulosa is considered a stenothermal species from a warm environment and has been reported in Texas and Florida, the West Indies, Mexico, Central America, and as far south as Brazil. According to the hypothesis of Hunter (1964), the capacity of this species for adaptation to different temperatures would not be expected to be as great as that of a eurythermal species. Stenothermal species are relatively limited by the environmental temperature, and therefore, one would expect a marked decrease in …

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