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2020

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Articles 241 - 270 of 1996

Full-Text Articles in Biology

Anti-Diabetic Activity Of Chrysophyllum Albidum (G. Don) Stem Bark In Alloxan-Induced Type 1 Diabetic Female Wistar Rats, Babalola Ola Yusuf, Musa Toyin Yakubu, Musbau Adewumi Akanji Oct 2020

Anti-Diabetic Activity Of Chrysophyllum Albidum (G. Don) Stem Bark In Alloxan-Induced Type 1 Diabetic Female Wistar Rats, Babalola Ola Yusuf, Musa Toyin Yakubu, Musbau Adewumi Akanji

Tanzania Journal of Science

Anti-diabetic activity of aqueous extract of Chrysophyllum albidum stem bark (AECASB) in female Wistar rats was investigated to confirm or refute the purported use and the underlying mechanism of action of C. albidum stem bark in the management of type 1 diabetes in Nigeria traditional medicine. Seventy female rats (180.60 ± 8.50 g) were assigned into seven groups. Rats in Group 1 (non-diabetic) were orally administered 1 mL of distilled water, while animals in Groups 2-7 were made diabetic and orally administered 1 mL of distilled water, 2.5 mg/kg body weight (bwt) of glibenclamide, 25, 50, 100 and 200 mg/kg …

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Spiny Mice (Acomys) Exhibit Attenuated Hallmarks Of Aging And Rapid Cell Turnover After Uv Exposure In The Skin Epidermis, Wesley Wong, Austin Kim, James R. Monaghan, Ashley W. Seifert, Malcolm Maden, Justin D. Crane Oct 2020

Spiny Mice (Acomys) Exhibit Attenuated Hallmarks Of Aging And Rapid Cell Turnover After Uv Exposure In The Skin Epidermis, Wesley Wong, Austin Kim, James R. Monaghan, Ashley W. Seifert, Malcolm Maden, Justin D. Crane

Biology Faculty Publications

The study of long-lived and regenerative animal models has revealed diverse protective responses to stressors such as aging and tissue injury. Spiny mice (Acomys) are a unique mammalian model of skin wound regeneration, but their response to other types of physiological skin damage has not been investigated. In this study, we examine how spiny mouse skin responds to acute UVB damage or chronological aging compared to non-regenerative C57Bl/6 mice (M. musculus). We find that, compared to M. musculus, the skin epidermis in A. cahirinus experiences a similar UVB-induced increase in basal cell …

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Patterning A Leaf By Establishing Polarities, Darren Manuela, Mingli Xu Oct 2020

Patterning A Leaf By Establishing Polarities, Darren Manuela, Mingli Xu

Faculty Publications

Leaves are the major organ for photosynthesis in most land plants, and leaf structure is optimized for the maximum capture of sunlight and gas exchange. Three polarity axes, the adaxial–abaxial axis, the proximal-distal axis, and the medial-lateral axis are established during leaf development to give rise to a flattened lamina with a large area for photosynthesis and blades that are extended on petioles for maximum sunlight. Adaxial cells are elongated, tightly packed cells with many chloroplasts, and their fate is specified by HD-ZIP III and related factors. Abaxial cells are rounder and loosely packed cells and their fate is established …

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Modeling The Load Of Sars-Cov-2 Virus In Human Expelled Particles During Coughing And Speaking, Yang Wang, Guang Xu, Yue-Wern Huang Oct 2020

Modeling The Load Of Sars-Cov-2 Virus In Human Expelled Particles During Coughing And Speaking, Yang Wang, Guang Xu, Yue-Wern Huang

Civil, Architectural and Environmental Engineering Faculty Research & Creative Works

Particle size is an essential factor when considering the fate and transport of virus-containing droplets expelled by human, because it determines the deposition pattern in the human respiratory system and the evolution of droplets by evaporation and gravitational settling. However, the evolution of virus-containing droplets and the size-dependent viral load have not been studied in detail. The lack of this information leads to uncertainties in understanding the airborne transmission of respiratory diseases, such as the COVID-19. In this study, through a set of differential equations describing the evolution of respiratory droplets and by using the SARS-CoV-2 virus as an example, …

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Succession In Native Groundwater Microbial Communities In Response To Effluent Wastewater, Chelsea M. Dinon Oct 2020

Succession In Native Groundwater Microbial Communities In Response To Effluent Wastewater, Chelsea M. Dinon

USF Tampa Graduate Theses and Dissertations

Aquifer storage and recovery techniques are used globally to mitigate increasing demands for groundwater. Microcosms were used to evaluate the effect of wastewater effluent that may be used for aquifer storage and recovery injection on native aquifer microbial communities. Sulfur Springs water was used as the source of aquifer water. The microcosms were 100% spring water, 100% unchlorinated filtered wastewater effluent, or one of three mixtures of unchlorinated filtered wastewater effluent and spring water. The mixture microcosms were 50%, 30%, and 10% unchlorinated filtered wastewater effluent with the balance as spring water. The effluent water was UV treated prior to …

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Making A First Impression: Exploring What Instructors Do And Say On The First Day Of Introductory Stem Courses, A. Kelly Lane, Clara L. Meaders, J. Kenny Shuman, Mackenzie R. Stetzer, Erin L. Vinson, Brian A. Couch, Michelle K. Smith, Marilyne Stains Oct 2020

Making A First Impression: Exploring What Instructors Do And Say On The First Day Of Introductory Stem Courses, A. Kelly Lane, Clara L. Meaders, J. Kenny Shuman, Mackenzie R. Stetzer, Erin L. Vinson, Brian A. Couch, Michelle K. Smith, Marilyne Stains

School of Biological Sciences: Faculty Publications

Student impressions formed during the first day of class can impact course satisfaction and performance. Despite its potential importance, little is known about how instructors format the first day of class. Here, we report on observations of the first day of class in 23 introductory science, technology, engineering, and math (STEM) courses. We first described how introductory STEM instructors structure their class time by characterizing topics covered on the first day through inductive coding of class videos. We found that all instructors discussed policies and basic information. However, a cluster analysis revealed two groups of instructors who differed primarily in …

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Atlantic Salmon (Salmo Salar) Neurocranium, Connor Depriest, Nadia Abeido, David Kerstetter Oct 2020

Atlantic Salmon (Salmo Salar) Neurocranium, Connor Depriest, Nadia Abeido, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

9.0 cm in length. Neurocranium scan of an Atlantic Salmon (Salmo salar). Collected from Two Bills Seafood on 04/09/20 by Nadia Abeido and Connor Depriest.

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Comparable Human Reconstitution Following Cesium-137 Versus X-Ray Irradiation Preconditioning In Immunodeficient Nog Mice, Anna Halling Folkmar Andersen, Stine Sofie Frank Nielsen, Rikka Olesen, Jakob Le Fèvre Harslund, Ole S. Søgaard, Lars Østergaard, Paul Denton, Martin Tolstrup Oct 2020

Comparable Human Reconstitution Following Cesium-137 Versus X-Ray Irradiation Preconditioning In Immunodeficient Nog Mice, Anna Halling Folkmar Andersen, Stine Sofie Frank Nielsen, Rikka Olesen, Jakob Le Fèvre Harslund, Ole S. Søgaard, Lars Østergaard, Paul Denton, Martin Tolstrup

Biology Faculty Publications

Humanized mouse models are used extensively in research involving human pathogens and diseases. However, most of these models require preconditioning. Radio-active sources have been used routinely for this purpose but safety issues have motivated researchers to transition to chemical or X-ray based preconditioning. In this study, we directly compare 350 kV X-ray and Cs-137 low-dose precondition of NOG mice before human stem cell transplantation. Based on flow cytometry data, we found that engraftment of human cells into the mouse bone marrow was similar between radiation sources. Likewise, human engraftment in the peripheral blood was comparable between Cs-137 and three different …

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Hepatobiliary Polycyclic Aromatic Hydrocarbons In Pelagic Fishes Of The Gulf Of Mexico, Madison R. Schwaab Oct 2020

Hepatobiliary Polycyclic Aromatic Hydrocarbons In Pelagic Fishes Of The Gulf Of Mexico, Madison R. Schwaab

USF Tampa Graduate Theses and Dissertations

Fisheries populations and their ecosystems are negatively impacted by both chronic and acute inputs of pollutants, including oil spills such as the Deepwater Horizon platform blowout in 2010 in the Gulf of Mexico (GoM). After Deepwater Horizon, toxicological studies of demersal fishes of the northern GoM were undertaken to characterize impacts and to establish baseline contaminant levels in the aftermath of the spill. In this study, I quantify polycyclic aromatic hydrocarbon (PAH) concentrations in eight pelagic fish species to demonstrate oil exposure differences between species, region and time. Analysis of biliary PAH metabolite equivalents using high performance liquid chromatography was …

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Hypogean Communities As Cybernetic Systems: Implications For The Evolution Of Cave Biotas, Aldemaro Romero Jr. Oct 2020

Hypogean Communities As Cybernetic Systems: Implications For The Evolution Of Cave Biotas, Aldemaro Romero Jr.

Publications and Research

Ramón Margalef proposed in 1968 that ecosystems could be better understood if they were viewed as cybernetic systems. I tested this hypothesis in the case of hypogean ecosystems using available pieces of evidence. I looked on how information on feedbacks, stability, succession, organization, diversity, and energy flows in the hypogean environment fit the cybernetics hypothesis. The results were that there are convincing arguments that the application of the concept of cybernetics in biospeleology can be beneficial to broadening our understanding of cave biota in terms of their structure. I also make the case that this approach can provide more clarity …

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Population Genetics Of Rhizophora Apiculata In Peninsular Malaysia Using Microsatellite Markers, Amelia Azman Oct 2020

Population Genetics Of Rhizophora Apiculata In Peninsular Malaysia Using Microsatellite Markers, Amelia Azman

Student Works (2020-2029)

Rampant illegal logging, overharvesting and deforestation coupled with climate change, pose significant threats to the natural stands of Rhizophora apiculata, or locally known as Bakau Minyak; one of the most economically and ecologically important species of mangroves in Peninsular Malaysia. Despite being a dominant mangrove species, the reduction in the number of R. apiculata in its habitat has resulted in concerns over the long-term survival potential of the species. In Malaysia, genetic information to develop effective guidelines for the conservation and management of mangrove species has been lacking, and hence, further research should be conducted to fill this gap. The …

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Science Alive, Shweta Zinzuwadia Oct 2020

Science Alive, Shweta Zinzuwadia

Trick to the Treat of Internships and Research

Science Alive is a not-for-profit organization based at NSU started by Dr. Emily Schmitt Lavin and Dr. Aarti Raja. This organization promotes community outreach and engagement by volunteering at Nova Blanche Forman Elementary School. Through this weekly afterschool program, NSU students promote and encourage STEM-related topics to the elementary students through various interactive activities. The main goal of Science Alive is to get kids excited about Science and be engaged within the Broward Community. If you have any questions, please email Shweta Zinzuwadia ([email protected]) or Dr. Schmit ([email protected])

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Clear Vision Center Internship, Maria Sanchez Oct 2020

Clear Vision Center Internship, Maria Sanchez

Trick to the Treat of Internships and Research

Come to learn more about my experience at Clear Vision Center as I gained first hand experiences working with patients and immersing myself within a healthcare setting.

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Development Of “Sharkavir”: A New Hypothetical Inhibitor For Hiv-1 Protease, Feza Abbas Oct 2020

Development Of “Sharkavir”: A New Hypothetical Inhibitor For Hiv-1 Protease, Feza Abbas

Trick to the Treat of Internships and Research

HIV has infected approximately 37 million people worldwide and results in over 1 million deaths annually. A key component of the HIV life cycle is the enzyme HIV-1 protease, which is involved in cutting newly made protein components of the HIV virion. Our research focused on how can molecular models be used to simulate the binding of various HIV-1 prtease inhibitors including Darunavir, Ritonavir, and a novel combination of these two named "Sharkavir".

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Effects Of Porphyromonas Gingivalis Derived Sphingolipids On Osteoclastogenesis, Geena Song, Michelle Hoang Oct 2020

Effects Of Porphyromonas Gingivalis Derived Sphingolipids On Osteoclastogenesis, Geena Song, Michelle Hoang

Trick to the Treat of Internships and Research

This was an independent study focused on literary research about the effect of Porphyromonas gingivalis on osteoclastogenesis. This opportunity was gained by emailing Jeff Hartman ([email protected]) and setting up a meeting with the professor, Dr. Movila. We gathered variety of research papers and analyzed it to write a peer reviewed article ourselves. Throughout this experience, we learned variety of skills, such as organizing materials into a coherent narrative and learned materials that showed up in future classes, such as microbiology and cellular molecular biology.

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Impact Of Covid-19 On Global Education And Research In Healthcare And Stem, Stephanie Autore, Jessica Hallet, Michelle Hoang, Santanu De Oct 2020

Impact Of Covid-19 On Global Education And Research In Healthcare And Stem, Stephanie Autore, Jessica Hallet, Michelle Hoang, Santanu De

Trick to the Treat of Internships and Research

The independent study focused on Covid-19 from the perspectives of global education and research in healthcare and STEM. Our aim was to determine the impact of the pandemic on each global sector and the associated transition to virtual modes. The research was conducted using peer-reviewed articles and papers. Each week, our group held zoom meetings to present our research and provide peer feedback. At the end of the experience, we all wrote three separate manuscripts on the topics regarding Covid 19 and global education, research in healthcare, and research in STEM.

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Medical Scribe Experience, Naziba A. Nuha Oct 2020

Medical Scribe Experience, Naziba A. Nuha

Trick to the Treat of Internships and Research

A medical scribe is a trained medical information manager who specializes in charting physician-patient encounters in real-time during medical exams. Come to learn more about my experience at Scribe America.

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Dermatology Office, Michelle Volis Oct 2020

Dermatology Office, Michelle Volis

Trick to the Treat of Internships and Research

I intern at a local dermatology office where I primarily shadow the head dermatologist but also shadow another dermatologist, a nurse practitioner, and a physician assistant. I shadow surgical procedures such as Mohs surgery which is a technique used to remove cancerous cells from the skin one layer at a time for the purpose of preserving healthy tissue. I also shadow cosmetic surgeries like injections and laser procedures. During my internship, there have been interesting clinical cases, so I am working on publishing articles about them with the help of the head dermatologist. I have also spent time in the …

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Expression & Localization Of The 14-3-3 Protein Family Within Mammals, Neha Kumrah, Santanu De Oct 2020

Expression & Localization Of The 14-3-3 Protein Family Within Mammals, Neha Kumrah, Santanu De

Trick to the Treat of Internships and Research

This past summer, I was able to complete a remote Independent Study with Dr. Santanu De. As a team of four, we interacted via Zoom meetings and shared documents. Together, we researched and analyzed the 14-3-3 protein family, a class of molecular chaperones found in essentially all mammals. I was able to conclude that this protein family has many implications in cellular biology, and that expression levels could potentially be used as a diagnostic tool for various cancers. Overall, this was a beneficial experience as I was able to strengthen my research skills, publish a manuscript, and gain a newfound …

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Survival And Development Of Zebra Longwing, Heliconius Charithonia, On Native And Non-Native Passion Vines, Passiflora Sp., In South Florida, Madelyn Rinka Oct 2020

Survival And Development Of Zebra Longwing, Heliconius Charithonia, On Native And Non-Native Passion Vines, Passiflora Sp., In South Florida, Madelyn Rinka

Trick to the Treat of Internships and Research

Working on this project since last fall has given me a lot of experience regarding writing abstracts, making presentations, following methodologies, and much more. Also, I've learned about the challenges that can go hand in hand with research in environmental science, including the unpredictability of finding a specific species in nature and the potential roadblocks caused by weather. The project was accepted for NSU's Undergraduate Student Symposium for Winter 2020 and the Florida Native Plant Society's Annual Conference (both were cancelled due to COVID-19). Overall, working on this project has allowed me to get a better understanding of research in …

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Functions Of 14-3-3 Proteins And Their Isoforms Across Various Mammaliam Species, Cells, Tissues, Organs And Developmental Stages, Elizabeth Barley, Santanu De Oct 2020

Functions Of 14-3-3 Proteins And Their Isoforms Across Various Mammaliam Species, Cells, Tissues, Organs And Developmental Stages, Elizabeth Barley, Santanu De

Trick to the Treat of Internships and Research

Throughout the Fall 2020 term, I had the opportunity to partake in an internship in biology with Dr. Santanu De as my faculty sponsor. The study that I completed focused on the 14-3-3 protein isoforms in various mammalian species, cells, tissues, organs and developmental stages. This experience allowed me to delve deeper into a topic in biology which supported my field of interest. Completing this study provided me with useful knowledge on an upper-level biology topic while providing me with a guided space to nurture my continued learning. The 14-3-3 proteins consist of seven isoforms in various mammalian species. My …

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Inheritability Of Ciprofloxacin-Induced Mitochondrial Mutations From Parental To Offspring Generation Using Quantitative Polymerase Chain Reaction, Rose Mccoy Oct 2020

Inheritability Of Ciprofloxacin-Induced Mitochondrial Mutations From Parental To Offspring Generation Using Quantitative Polymerase Chain Reaction, Rose Mccoy

Honors Program: Senior Projects (Public)

In all eukaryotes, mitochondria are known as the powerhouse of the cell (Siekevitz, 1957). In plants, however, their mitochondrial genome is especially strange. Plant mitochondrial genomes are extremely large and contain both linear and circular subgenomic DNA fragments. Plant mitochondrial genomes undergo a significant amount of mutations in the form of rearrangements. However, it is not known how often these rearrangements are inherited by the next generation. It is thought that plant cells that are still dividing have higher rates of DNA repair, such as double-strand break repair, to ensure the quality of that plant lineage. As follows, it is …

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Identification Of Planktothrix (Cyanobacteria) Blooms And Effects On The Aquatic Macroinvertebrate Community In The Non-Tidal Potomac River, Usa, Joshua Henesy, Jennifer L. Wolny, John E. Mullican, Detbra S. Rosales, Joseph S. Pitula, Joseph W. Love Oct 2020

Identification Of Planktothrix (Cyanobacteria) Blooms And Effects On The Aquatic Macroinvertebrate Community In The Non-Tidal Potomac River, Usa, Joshua Henesy, Jennifer L. Wolny, John E. Mullican, Detbra S. Rosales, Joseph S. Pitula, Joseph W. Love

Virginia Journal of Science

Using transverse cross-sectional transects, a survey of 31 km of the non-tidal Potomac River was conducted from White’s Ferry, Virginia to Brunswick, Maryland, USA, between June and September in 2013 through 2015 to assess a recurring benthic cyanobacteria bloom. Abundant benthic cyanobacteria blooms were detected during the 2014 and 2015 sampling seasons and the primary taxon was identified morphologically and molecularly as Planktothrix cf. isothrix. When present, P. cf. isothrix blooms were concentrated from river center to the Maryland shoreline. This pattern was correlated with significantly greater benthic chlorophyll-a and phycocyanin concentrations. In an apparent response to …

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Axonal Blockage With Microscopic Magnetic Stimulation, Hui Ye Oct 2020

Axonal Blockage With Microscopic Magnetic Stimulation, Hui Ye

Biology: Faculty Publications and Other Works

Numerous neurological dysfunctions are characterized by undesirable nerve activity. By providing reversible nerve blockage, electric stimulation with an implanted electrode holds promise in the treatment of these conditions. However, there are several limitations to its application, including poor bio-compatibility and decreased efficacy during chronic implantation. A magnetic coil of miniature size can mitigate some of these problems, by coating it with biocompatible material for chronic implantation. However, it is unknown if miniature coils could be effective in axonal blockage and, if so, what the underlying mechanisms are. Here we demonstrate that a submillimeter magnetic coil can reversibly block action potentials …

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Draft Genome Of Bugula Neritina, A Colonial Animal Packing Powerful Symbionts And Potential Medicines, Mikhail Rayko, Aleksey Komissarov, Jason C. Kwan, Grace Lim-Fong, Adelaide C. Rhodes, Sergey Kliver, Polina Kuchur, Stephen James O'Brien, Jose Lopez Oct 2020

Draft Genome Of Bugula Neritina, A Colonial Animal Packing Powerful Symbionts And Potential Medicines, Mikhail Rayko, Aleksey Komissarov, Jason C. Kwan, Grace Lim-Fong, Adelaide C. Rhodes, Sergey Kliver, Polina Kuchur, Stephen James O'Brien, Jose Lopez

Biology Faculty Articles

Many animal phyla have no representatives within the catalog of whole metazoan genome sequences. This dataset fills in one gap in the genome knowledge of animal phyla with a draft genome of Bugula neritina (phylum Bryozoa). Interest in this species spans ecology and biomedical sciences because B. neritina is the natural source of bioactive compounds called bryostatins. Here we present a draft assembly of the B. neritina genome obtained from PacBio and Illumina HiSeq data, as well as genes and proteins predicted de novo and verified using transcriptome data, along with the functional annotation. These sequences will permit a better …

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Tadpole Of The Amazonia Frog Edalorhina Perezi (Anura: Leptodactylidae) With Description Of Oral Internal And Chondrocranial Morphology, Filipe A.C Do Nascimento, Rafael O. De Sá, Paulo C. De A. Garcia Oct 2020

Tadpole Of The Amazonia Frog Edalorhina Perezi (Anura: Leptodactylidae) With Description Of Oral Internal And Chondrocranial Morphology, Filipe A.C Do Nascimento, Rafael O. De Sá, Paulo C. De A. Garcia

Biology Faculty Publications

The genus Edalorhina consists of two species of small forest-floor frogs inhabiting the Amazon basin. The tadpole of Edalorhina perezi, the most widely distributed species, was previously described based on a single and early stage (Gosner 25) individual. Herein, we provide a description of the tadpole in Gosner stages 35–36 including internal morphology data (i.e., buccopharyngeal cavity and larval skeleton) based on samples from two populations from Ecuador. Edalorhina shares a generalized morphology with most members of its closely related taxa; however, it is distinguished from the other species by having an almost terminal oral disc. The presence of …

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Survivor Microbial Populations In Post-Chlorinated Wastewater Are Strongly Associated With Untreated Hospital Sewage And Include Ceftazidime And Meropenem Resistant Populations, Rachelle E. Beattie, Troy Skwor, Krassimira R. Hristova Oct 2020

Survivor Microbial Populations In Post-Chlorinated Wastewater Are Strongly Associated With Untreated Hospital Sewage And Include Ceftazidime And Meropenem Resistant Populations, Rachelle E. Beattie, Troy Skwor, Krassimira R. Hristova

Biological Sciences Faculty Research and Publications

Wastewater treatment plant (WWTP) effluent has been implicated in the spread of antibiotic resistant bacteria (ARB), including pathogens, as the WWTP environment contains multiple selective pressures that may increase mutation rates, pathogen survivability, and induce gene transfer between bacteria. In WWTPs receiving hospital sewage, this selective effect may be more pronounced due to increased concentrations of antibiotics, ARB, and clinical pathogens from hospital sewage. To determine the extent to which hospital sewage contributes to the microbial community of disinfected wastewater which is released into the environment, we used 16S rRNA sequencing of hospital sewage, WWTP influent, primary effluent, Post-Chlorinated Effluent, …

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Novel Information About The Kinetic Effects Of Equine Shoe Modifications And Kinematic Effects Of Human Digital Devices For Improved Performance In Both Species, Pengju Wang Oct 2020

Novel Information About The Kinetic Effects Of Equine Shoe Modifications And Kinematic Effects Of Human Digital Devices For Improved Performance In Both Species, Pengju Wang

LSU Master's Theses

Equine shoes are frequently modified to enhance traction for horses that travel on paved surfaces for work, pleasure, or entertainment. Little is known about other common shoe modifications used to enhance traction like calks, tungsten carbide granules, or plastic composition. This information is vital to shoe design to protect the safety and welfare of all service, working, and leisure horses. The objective of the first part of this thesis was to quantify the effect of shoes with and without traction adaptions on kinetic measures in non-lame, light breed horses at a trot. Kinetic data was collected with a force platform …

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Analyzing Flavonoids In Chocolate-Based Beverages, Ernest Afoakwah Oct 2020

Analyzing Flavonoids In Chocolate-Based Beverages, Ernest Afoakwah

Kean Quest

Some amount of previous research has suggested that chocolate-based drinks are very effective post-exercise recovery beverages. The fluid, electrolyte, protein and carbohydrate content of chocolate milk has been credited with possibly providing this benefit. Antioxidants perhaps contribute to post exercise recovery. Flavonoids, also naturally occurring phytochemicals in cocoa, the chief ingredient in chocolate, may be capable of providing antioxidant benefits. Using high-performance liquid chromatography (HPLC) and colorimetry, the present research involved examining the presence of flavonoids (particularly catechin) in seven chocolate-based milk drinks, as they may serve some importance regarding post-exercise recovery. A similar protocol (which basically involved defatting samples, …

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Identification And Characterization Of Novel Genes And Genetic Interactions That Influence Iba Metabolism, Vanessica Jawahir Oct 2020

Identification And Characterization Of Novel Genes And Genetic Interactions That Influence Iba Metabolism, Vanessica Jawahir

Dissertations

Indole-3-butyric acid (IBA) is an endogenous storage auxin important for maintaining appropriate indole-3-acetic acid (IAA) levels that influences primary root elongation and lateral root development. IBA is metabolized into free IAA in the peroxisome in a multistep process similar to fatty acid β-oxidation. Although many components specific to IBA metabolism and peroxisome function have been identified, our understanding is incomplete. I sought to identify novel components of IBA metabolism or peroxisome function by conducting a forward genetic screen for Arabidopsis thaliana plants with enhanced resistance to IBA. I identified Long chain acyl-CoA synthetase 4 (LACS4) as a novel gene functioning …

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