Biology Commons^™

High-Throughput Screen Of Drug Repurposing Library Identifies Inhibitors Of Sarcocystis Neurona Growth, Gregory D. Bowden, Kirkwood M. Land, Roberta M. O'Connor, Heather M. Fritz

College of the Pacific Faculty Articles

The apicomplexan parasite Sarcocystis neurona is the primary etiologic agent of equine protozoal myeloencephalitis (EPM), a serious neurologic disease of horses. Many horses in the U.S. are at risk of developing EPM; approximately 50% of all horses in the U.S. have been exposed to S. neurona and treatments for EPM are 60-70% effective. Advancement of treatment requires new technology to identify new drugs for EPM. To address this critical need, we developed, validated, and implemented a high-throughput screen to test 725 FDA-approved compounds from the NIH clinical collections library for anti-S. neurona activity. Our screen identified 18 compounds with confirmed …

Small Mammal Diversity, Rattlesnake Demographics, And Resource Utilization In The Great Basin: Implications For Management And Stable Isotope Proxies, Bryan T. Hamilton

Theses and Dissertations

Plant carbon isotopes were used to track assimilation of riparian resources by small mammals. Voles and shrews derived significant portions of their carbon from riparian vegetation. Deer and harvest mice were abundant in riparian habitat but assimilated little riparian vegetation indicating that the riparian corridor provided resources other than food. This is first use of stable carbon isotopes to trace riparian resources into a vertebrate community. Conifer encroachment in sagebrush ecosystems negatively affects many wildlife populations. Conifer removal is recommended across millions of hectares in the Great Basin. However the effects of conifer encroachment and conifer removal are unknown for …

The Relationship Between Site Contamination And Native Plant Success In Butte, Mt: Implications For Future Restoration, Jared Trilling

Graduate Theses & Non-Theses

Interest in ecological restoration of mining impacted areas, as opposed to traditional reclamation practices, has been increasing in recent years. Holistic ecological restoration is preferable to traditional reclamation practices as it provides better ecological function, biodiversity, and human enjoyment. However, legacy effects of mining, such as metals contamination and poor growing substrates, has hampered restoration efforts and further, there is little research on primary succession of these novel ecosystems. There is also scant information on many native plant species responses to contamination.

This study focuses on developing plant-contaminant relationships to guide restoration planting decisions. We look at Butte, MT, an …

Moss Revegetation- A Process To Initiate Restoration And Repair Natural Resources Damaged By Mining, Arundeep Taduri

Graduate Theses & Non-Theses

Mosses are small plants within the phylum Bryophyta known for their ability to grow on acidic, metal laden soil. This project proposes the restoration of moss to barren mine waste areas which will stabilize the soil, thus reducing heavy metal contaminants released to the aquifer and the surrounding environment.

In general, the mine waste areas can be naturally recolonization by native and non-native vegetation as a remediation process. This process is very slow. The removal of the mine waste, capping and replacing it with clean soil, and subsequently replanting is a common remediation method. However, this approach is very expensive …

Using Multi‐Response Models To Investigate Pathogen Coinfections Across Scales: Insights From Emerging Diseases Of Amphibians, William E. Stutz, Andrew R. Blaustein, Cheryl J. Briggs, Jason T. Hoverman, Jason R. Rohr, Pieter T. J. Johnson

Integrative Biology Faculty and Staff Publications

1. Associations among parasites affect many aspects of host–parasite dynamics, but a lack of analytical tools has limited investigations of parasite correlations in observational data that are often nested across spatial and biological scales.
2. Here we illustrate how hierarchical, multiresponse modelling can characterize parasite associations by allowing for hierarchical structuring, offering estimates of uncertainty and incorporating correlational model structures. After introducing the general approach, we apply this framework to investigate coinfections among four amphibian parasites (the trematodes Ribeiroia ondatrae and Echinostoma spp., the chytrid fungus Batrachochytrium dendrobatidis and ranaviruses) and among >2,000 individual hosts, 90 study sites and five amphibian host …

Herpetofauna Of Sumak Allpa: A Baseline Assessment Of An Unstudied Island Herpetofaunal Community, Sara Freimuth

Independent Study Project (ISP) Collection

Sumak Allpa is an island dedicated to the provision and protection of habitat for the conservation and rehabilitation of primates. As such, the island - a varzea ecosystem located in the Western Amazon of Ecuador, one of the most biodiverse and also most threatened regions in the world – consists of protected primary forest that is home not only to a variety of primates, but also to an even wider variety of other taxa, nearly all of which have gone unstudied on the island. The present investigation assessed two of those taxa, amphibians and reptiles, in order to establish a …

If You Build It, They Will Come: A Habitat Mimicry-Based Assessment Tool For Estimating Larval Salamander Density In Temperate Forests Streams, Margaret E. Maloney

Honors Theses

The biology of small, forested streams is critical to the ecology of larger waterways and broader watershed function. Because of their size, these “headwater streams” are strongly influenced by surrounding landscape conditions and their biology is intimately connected to the landscape through material and energy subsidies. In the United States, 50 to 80 percent of streams are primary headwater streams, making them a high priority for conservation. Often, stream salamanders are a useful indicator for biotic integrity of headwater streams due to their longevity, relatively stable populations, small home ranges, and abundance. Assessing stream salamanders is a challenging endeavor and …

The Evolutionary History Of Nebraska Deer Mice: Local Adaptation In The Face Of Strong Gene Flow, Susanne P Pfeifer, Stefan Laurent, Vitor C. Sousa, Catherine R. Linnen, Matthieu Foll, Laurent Excoffier, Hopi E. Hoekstra, Jeffrey D. Jensen

Biology Faculty Publications

The interplay of gene flow, genetic drift, and local selective pressure is a dynamic process that has been well studied from a theoretical perspective over the last century. Wright and Haldane laid the foundation for expectations under an island-continent model, demonstrating that an island-specific beneficial allele may be maintained locally if the selection coefficient is larger than the rate of migration of the ancestral allele from the continent. Subsequent extensions of this model have provided considerably more insight. Yet, connecting theoretical results with empirical data has proven challenging, owing to a lack of information on the relationship between genotype, phenotype, …

Volume 10, Taylor Hogg, Tiffany Carter, Brandyn Johnson, Haleigh James, Josh Baker, Tyler Cernak, Kirsten Bauer, Allie Snavely, Mary Zell Galen, Eric Powell, Thomas Wise, Katie Kinsey, Beth Barbolla, Maeleigh Ferlet, Rebecca Morra, Michala Day, Alexandra Evangelista, Max Flores, Harley Hodges, Clardene Jones, Harrison Samaniego, Jamesha Watson, Abby Gargiulo, Heather Green, Haley Klepatzki, Juan Guevara, Dani Bondurant, Michael Joseph Link Jr., Pamela Dahl, Maeve Losen, Charlotte Murphey

Incite: The Journal of Undergraduate Scholarship

Introduction Dr. Roger A. Byrne

An Analysis of Media Framing in Cases of Violence Against Women by Taylor Hogg

Writing in the Discipline of Nursing by Tiffany Carter

Photography by Brandyn Johnson

The Hidden Life of Beef Cattle: A Study of Cattle Welfare on Traditional Ranches and Industrial Farms by Haleigh James

Bloodworth's by Josh Baker and Tyler Cernak

Prosimians: Little Bodies, Big Significance by Kirsten Bauer

Skinformed by Allie Snavely

Coopertition and Gracious Professionalism: The Effects of First Robotics Folklore and Culture on the Stem Community by Mary Zell Galen

Tilt by Eric Powell And Thomas Wise

The Millennial …

Effects Of Radiation On Neurite Morphology And Cytoskeleton Structure, Melissa Sassman

Student Scholarship

Long-term manned space exploration to the moon, Mars, and other areas beyond Earth's protective magnetic field poses possible acute and late central nervous systems (CNS) risks. Of particular concern for astronauts is exposure to high atomic number, high energy particles known as HZE particles, a component of galactic cosmic radiation (GCR). Although NASA has radiation safety requirements, the possible effects of GCR and HZE particles on the central nervous systems of astronauts remains unknown. Understanding the risks and effects of galactic cosmic radiation and HZE particles on the central nervous system will allow for safer space exploration.

Additionally, and perhaps …

Defining A Species: A Look At The Relationship Of Papio And Theropithecus Using Alu Elements, Paulina Gonzalez-Quiroga

Honors Capstones

No abstract provided.

Infection Kinetics Of Rickettsia Asemboensis And Interaction With Rickettsia Felis Within The Cat Flea (Ctenocephalides Felis), Sabrina Magdalena Camille Valdes

Honors Capstones

No abstract provided.

Inhibition Of Carboxyltransferase From Escherichia Coli By Oxadiazolopyrazines, Margaret Mccarron

Honors Capstones

No abstract provided.

Epigenetic Misregulation As A Causal Agent Of Disease: Methylation Analysis And Rna Expression Quantification, Carter Pesson

Honors Capstones

No abstract provided.

Analysis Of Protein-Protein Interactions Between The Polybromo Subunit Of A Drosophila Melanogaster Chromatin Remodeling Complex And The Beaf Insulator Protein By Yeast-Two-Hybrid Assay, John Selloriquez

Honors Capstones

No abstract provided.

Biochemical Characterization Of Fe-S Cluster Assembly Machinery In Methanogenic Archaea, Nicole Bryer

Honors Capstones

No abstract provided.

3d Modeling And Animation Of The Temporomandibular Joint (Tmj) Of A Human Male With Implications For The Study Of Tmj Dysfunction, Breigh Sonnier

Honors Capstones

No abstract provided.

An Investigation Of Maltose Transport System Induction In The Rugose Phase Variant Of Human Pathogen Vibrio Vulnificus, Hira Hasan

Honors Capstones

No abstract provided.

Artemisia Scoparia Reduces The Effects Of Tnfα-Induced Lipolysis In Murine Adipocytes, Ruth Dunn

Honors Capstones

No abstract provided.

Analysis Of Young Alu Elements In The Squirrel Monkey (Saimiri) Lineage For Use As Species-Indicative Molecular Markers, Michael W. Denham

Honors Capstones

No abstract provided.

Efficacy Of Compound X (Bedoukian Research, Inc.) On Reducing Fecal Egg Count In Lambs, Mary M. France

Honors Capstones

No abstract provided.

Ph Dependence Of Dna Binding By Dna Polymerase I From Pychromonas Ingrahamii And Single Stranded Binding Protein From Deinococcus Radiodurans, Olivia Leonovicz

Honors Capstones

No abstract provided.

Sine Language: Communicating Platy-1 Retrotransposon Propagation In The Aotus Lineage, Jackson Mierl

Honors Capstones

No abstract provided.

Changes In Neural Activation Between Pre-Release And Post-Release Mouth Brooding Astatotilapia Burtoni Females, Victoria Oanh Huynh

Honors Capstones

No abstract provided.

Determining The Role Of Fatty Acid Composition In Antibiotic Resistance, Andrew Deak

Honors Theses

Bacterial infections that can no longer be treated by antibiotics because of bacterial mutations cause many infections and deaths each year. My research conducted aims to study how membrane fatty acid composition can affect membrane susceptibility to antibiotics. Listeria monocytogenes, a gram-negative facultative anaerobe, is the bacterium that I am testing. Listeria has 80-90% branched-chain fatty acids (BCFAs) which allow membrane fluidity and sufficient protection against invaders. When Listeria is grown in the presence of butyrate, the BCFAs become straight-chain fatty acids (SCFAs) and make the once fluid membrane more rigid. This allows for easier antibiotic penetration of the phospholipid …

Characterization Of The Glycosylation Of Aquaglyceroporin Hc-3 In Erythrocytes From The Freeze Tolerant Anuran, Dryophytes Chrysoscelis, Dante L. Pezzutti

Honors Theses

By utilizing an extreme physiological adaptation known as freeze-tolerance, Cope’s gray tree frog, Dryophytes chrysoscelis, freezes and then subsequently thaws up to 65% of its extracellular fluid to survive the winter. During these periods of freezing and thawing, erythrocytes (RBCs) of D. chrysoscelis utilize a protein, aquaglyceroporin HC-3, that facilitates transmembrane flux of both water and cryoprotective glycerol to mediate osmotic adjustments. RBCs from cold-acclimated tree frogs up-regulate HC-3 protein expression, which coincides with more abundant membrane localization and higher levels of glycosylation. However, the functional significance of HC-3 glycosylation on membrane localization and cellular freeze tolerance is currently not …

Editing Life: Modern Biology And Biblical Principles, Peter J. Lyons

Faculty Publications

No abstract provided.

The Effect Of Undergraduate Biology Research Experiences And Mentoring Structures On Student Self-Efficacy, Kyle Harris

Doctoral Dissertations and Projects

Collaborative inquiry within undergraduate research experiences (UREs) is an effective curriculum tool to support student growth. This study seeks to understand how collaborative inquiry within undergraduate biology student experiences are affected within faculty mentored experiences and non-mentored experiences at a large private southeastern university. Undergraduate biology students engaged in UREs (faculty as mentor and non-mentor experiences) were examined for statistically significant differences in student self-efficacy. Self-efficacy was measured in three subcomponents (thinking and working like a scientist, scientific self-efficacy, and scientific identity) from student responses obtained in an online survey. Responses were analyzed using a nonparametric equivalent of a t …

Evaluating Reproducibility In Computational Biology Research, Morgan Oneka

Honors Projects

For my Honors Senior Project, I read five research papers in the field of computational biology and attempted to reproduce the results. However, for the most part, this proved a challenge, as many details vital to utilizing relevant software and data had been excluded. Using Geir Kjetil Sandve's paper "Ten Simple Rules for Reproducible Computational Research" as a guide, I discuss how authors of these five papers did and did not obey these rules of reproducibility and how this affected my ability to reproduce their results.

Restoration Of Native-Dominated Plant Communities On A Centaurea Stoebe L.-Infested Site, Neil W. Macdonald, Kaitlyn M. Dykstra, Laurelin M. Martin

Open Access Publishing Support Funded Articles

Questions: Restoring native-dominated plant communities often requires controlling invasive species, reintroducing native species, and implementing continued management practices. Can single herbicide applications to control Centaurea stoebe L. encourage establishment of seeded native species more effectively than a single mowing? Can annual hand pulling to control C. stoebe favor the persistence of seeded native species? Can mid-spring burning reduce C. stoebe and increase native forbs and grasses? After eight years, will the restored plant communities differ from those in untreated areas?

Location: Bass River Recreation Area, Ottawa County, MI, USA.

Methods: We studied the effects of site preparation (mowing, clopyralid, glyphosate), …