Biology Commons^™

Sexual Differentiation Of Circadian Clock Function In The Adrenal Gland, Ian Kloehn, Savin B. Pillai, Laurel Officer, Claire Klement, Paul J. Gasser, Jennifer A. Evans

Biological Sciences Faculty Research and Publications

Sex differences in glucocorticoid production are associated with increased responsiveness of the adrenal gland in females. However, the adrenal-intrinsic mechanisms that establish sexual dimorphic function remain ill defined. Glucocorticoid production is gated at the molecular level by the circadian clock, which may contribute to sexual dimorphic adrenal function. Here we examine sex differences in the adrenal gland using an optical reporter of circadian clock function. Adrenal glands were cultured from male and female Period2::Luciferase (PER2::LUC) mice to assess clock function in vitro in real time. We confirm that there is a pronounced sex difference in the intrinsic capacity to …

Gender Differences In Space-Use Patterns And Microhabitat Characteristics Of Southern Flying Squirrel (Glaucomys Volans) In Northeastern Iowa, Elizabeth G. Bainbridge

Master's Theses or Doctor of Nursing Practice

Southern flying squirrel (Glaucomys volans) is common throughout the eastern deciduous forests of the United States, southern Canada, Mexico, and Central America. However, within the state of Iowa G. volans currently is listed as a “species of special concern.” This status is due to general loss of local habitat and lack of information about the species within the state. The state of Iowa has lost a majority of its native land cover over the past century due to intensive agricultural practices. Most native forests have been reduced drastically. The majority of habitat that would be suitable for southern flying squirrel …

Evaluating The Effect Of Water Supplementation On Ring-Necked Pheasant And Mesocarnivore Occupancy In Western Kansas, Brandon L. Calderon

Master's Theses or Doctor of Nursing Practice

A “guzzler” is any structure that stores and supplements water for wildlife populations. They are often used to target economically influential game species where water is thought to be potentially limiting. Upland game, like the ring-necked pheasant (Phasianus colchicus) in the semi-arid landscape of western Kansas, represent such populations as guzzlers have become common practice in wildlife management applications across the region, especially on lands enrolled in the Conservation Reserve Program (CRP). However, little is known about the spatial response, if any, of wildlife populations to guzzlers or the potential increased risk they pose for predation. From June to August …

Filifactor Alocis, A Newly Appreciated Oral Pathogen, Fails To Induce The Respiratory Burst Response Of Human Neutrophils., Jacob S. Edmisson

College of Arts & Sciences Senior Theses

Almost 50% of adult Americans suffer from periodontitis which is a bacterially induced inflammation of the tissue that surround and support the tooth. The accumulation of neutrophils, a critical cell component of the innate immune system, in the gingival crevice contributes to tissue damage. Filifactor alocis is a newly appreciated pathogen present in oral biofilms at periodontal disease sites. Studying the interactions between neutrophils and F. alocis will provide valuable information for delineating the role of this bacterium in periodontal disease and enhance our understanding of bacterial strategies to evade leukocytes’ antimicrobial mechanisms. The hypothesis that F. alocis modulates human …

Bipolar Cell Dendritic Morphology Analysis In Mice With Model Retinitis Pigmentosa., Simra Ahmed

College of Arts & Sciences Senior Theses

No abstract provided.

Role Of Dietary Fatty Acids In Liver Injury Caused By Vinyl Chloride Metabolites In Mice., Heegook Yeo

College of Arts & Sciences Senior Theses

Background. Vinyl chloride (VC) is an environmental pollutant found in many industrial sites and it ranks 4th on the 2013 Hazardous Substances Priority List published by Agency for Toxic Substances and Disease Registry’s (ATSDR). We have previously reported increased hepatocellular necrosis with high occupational VC exposure in human and in in vitro models; however, the effect of subtoxic VC exposure on liver with underlying conditions is unknown. The purpose of the current study is to investigate the hepatic injury caused by chloroethanol (CE; VC metabolite) and inflammatory response in two experimental models of high-fat diet (HFD) induced obesity. Methods. Mice …

Exploring The Mechanisms Of Action Of Antifungal Peptides Using Saccharomyces Cerevisiae., Michelle L. Mason

Biological Sciences Undergraduate Honors Theses

Candida albicans is a normal inhabitant of the skin and mucosal membranes of humans, however, in individuals with depressed immune systems or disrupted cutaneous flora, Candida can overgrow and cause serious infection. Candida infection is the fourth leading cause of nosocomial infection in the United States. These infections are often associated with longer hospital stays and higher mortality. Current drug therapies for this infection are largely ineffective due to the increased drug resistance of Candida species, and for some therapeutics, high levels of drug toxicity to humans. Histatin 5 is a naturally occurring salivary peptide that has strong antifungal properties. …

Rhebi Expression In Embryonic And Postnatal Mouse, Qi Tian, James L. Smart, Joachim H. Clement, Yingming Wang, Alex Derkatch, Harald Schubert, Michael V. Danilchik, Daniel L. Marks, Lev M. Fedorov

Faculty Publications - Department of Biological & Molecular Science

Ras homolog enriched in brain (RHEB1) is a member within the superfamily of GTP-binding proteins encoded by the RAS oncogenes. RHEB1 is located at the crossroad of several important pathways including the insulin-signaling pathways and thus plays an important role in different physiological processes. To understand better the physiological relevance of RHEB1 protein, the expres- sion pattern of RHEB1 was analyzed in both embryonic (at E3.5–E16.5) and adult (1-month old) mice. RHEB1 immu- nostaining and X-gal staining were used for wild-type and Rheb1 gene trap mutant mice, respectively. These inde- pendent methods revealed similar RHEB1 expression pat- terns during both …

Evaluating College Biology Laboratory Accommodations For Students With Blindness And Visual Impairments, Barbara Rae Heard

All Theses And Dissertations

Studies show that active participation in science laboratory activities promotes student learning. However, students with blindness and visual impairments (BVI) often confront obstacles to active participation in the required activities of the college biology laboratory. Legislation requires institutions of higher education to provide accommodations for students with disabilities, yet the institutions must also maintain the academic integrity of their courses and programs. While college biology instructors provide specific accommodations, such as tactile models and audible devices, to enable active participation by students with BVI, they do so without research-supported guidelines for best practices. This mixed methods study sought to evaluate …

The Skin Disease Mutation Cx31l209f Induces A Calcium- And Cobalt-Sensitive Leak Current Across The Plasma Membrane, Husain A. Bneed

Biology Theses

Gap junctions are essential to the function of multicellular animals, which require a high degree of coordination between cells. The functions of gap junctions are highly diverse and include exchange of metabolites and electrical signals between cells, as well as functions apparently unrelated to intercellular communication. In vertebrates, gap junctions are comprised of connexin proteins and over 20 connexins have been identified in humans. Each connexin is named according to its predicted molecular weight, for instance connexin31 (Cx31) has a predicted molecular weight of 31 KDa. Mutations in connexin genes cause a variety of disorders depending on the connexin protein …

The Effect Of Hypertonicity On Glucose Regulation In Healthy Adults, Alison L. Schroeder

Biological Sciences Undergraduate Honors Theses

Impaired glucose tolerance is the key defining characteristic of diabetes, a condition which affects nearly 30% of Americans. Hydration status and the physiological effects of poor hydration have been linked to compromised glucose tolerance in animal models, but have not been thoroughly investigated in humans. PURPOSE: The purpose of this investigation was to observe the effect of altered hydration status on a healthy male adult’s response to an oral glucose tolerance test (OGTT). METHODS: Four healthy adult men (28.4±1 y, 23.6±1.2 kg/m2, HbA1C 5.6±0.2%, non-smoking, non-athletes) participated. Subjects took OGTT’s in both a hypohydrated and euhydrated state. The euhydrated state …

Damsels In Distress: A Study Of The Effect Of Temperature On Damselfly Competitive Interactions, Madison Pitts

Biological Sciences Undergraduate Honors Theses

Temperature has a fundamental role in shaping numerous physiological aspects of organisms, such as growth rate, that determine an organism's performance. Due to global climate change, temperature is projected to keep increasing. As temperature increases, so does an organism’s growth rate. Also, as competition increases organisms’ growth rate decreases. While studies have examined these factors individually, no experiments have assessed the combined impact of temperature and competition on organism’s growth rates. The experiment we conducted manipulated temperature and density in order to project what will happen as a result of rising temperatures.

Assessing The Limitations And Capabilities Of Lidar And Landsat 8 To Estimate The Aboveground Vegetation Biomass And Cover In A Rangeland Ecosystem Using A Machine Learning Algorithm, Shital Dhakal

Boise State University Theses and Dissertations

Remote sensing based quantification of semiarid rangeland vegetation provides the large scale observations required for monitoring native plant distribution, estimating fuel loads, modeling climate and hydrological dynamics, and measuring carbon storage. Fine scale 3-dimensional vertical structural information from airborne lidar and improved signal to noise ratio and radiometric resolution of recent satellite imagery provide opportunities for refined measurements of vegetation structure.

In this study, we leverage a large number of time series Landsat 8 vegetation indices and lidar point cloud - based vegetation metrics with ground validation for scaling aboveground shrub and herb biomass and cover from small scale plot …

Phenotypic Characterization Of Adipose-Specific Vdr Knockout Mice, Joseph D'Angelo

Biological Sciences

Breast cancer is a prominent and lethal disease that currently affects close to three million people in the United States. Each year displays close to 250,000 new cases of breast cancer and around 40,000 deaths in the United States alone. A geographic pattern of breast cancer suggests a higher incidence in more temperate regions. Research has suggested that low Vitamin D levels associated with reduced sun exposure might contribute to increased breast cancer incidence. In recent studies, Vitamin D has been shown to slow the proliferation of breast cancer cells, but the mechanisms involved in vivo are poorly defined. It …

The Essential Oil Of Lippia Alba Affects Drosophila Behavior And Physiology, Jibin Manimala

Biological Sciences

Lippia alba (LA) is a flowering shrub native to Central and South America. Its essential oil has been used in herbal medicine as an anti-anxiety drug and in aquaculture; it is used to sedate fish during transport (Daniel 2014. Essential oils are derived from plants usually through steam distillation. They are labeled as “essential” because it contains the odor of the plant. Its physiological action is unknown. We use the fruitfly Drosophila melanogaster to further define its behavioral actions and determine its underlying physiological effects. We first tested whether Lippia alba produced had an anesthetic effect on the fruitfly. We …

Design For Mosquito Abundance, Diversity, And Phenology Sampling Within The National Ecological Observatory Network, D. Hoekman, Y. P. Springer, C. M. Barker, R. Barrera, M. S. Blackmore, W. E. Bradshaw, D. H. Foley, H. S. Ginsberg, M. H. Hayden, C. M. Holzapfel, S. A. Juliano, L. D. Kramer, S. L. Ladeau, Todd P. Livdahl, C. G. Moore, R. S. Nasci, W. K. Reisen, H. M. Savage

Biology

The National Ecological Observatory Network (NEON) intends to monitor mosquito populations across its broad geographical range of sites because of their prevalence in food webs, sensitivity to abiotic factors, and relevance for human health. We describe the design of mosquito population sampling in the context of NEON's long-term continental scale monitoring program, emphasizing the sampling design schedule, priorities, and collection methods. Freely available NEON data and associated field and laboratory samples, will increase our understanding of how mosquito abundance, demography, diversity, and phenology are responding to land use and climate change.

Erratum: In the paper by Hoekman et al. (“Design …

Green Tea Polyphenols : Potential Antibiotic Synergistic Agents For Combatting Antibiotic Resistant Bacteria, Siti Ayuni Mohamed Yussof

Theses, Dissertations and Culminating Projects

The rise in antibiotic resistant cases has caused a global concern and researchers around the world are trying to find a novel alternative to combat this issue. Green tea with its many health benefits, including antibacterial and antiviral activity, has shown to be one of the most promising candidates to be used as an agent to solve this problem. Green tea commonly found throughout Asia is made from the unfermented leaves of Camellia sinensis and the polyphenols extracted from it can be divided into two categories: (1): Crude, which includes green tea polyphenols (GTP) and lipophilic green tea polyphenols (LTP), …

Social And Scientific Implications Of Genetic Testing In The Digital Age, Yaruska A. Ordinola

Senior Honors Projects

From Mendel’s law of inheritance in the 19th century through Watson and Crick’s revolutionary observations of the double helix in the 20th century, genetics has been a fascinating and continuing topic of discussion in the field of science (Collins & McKusick, 2001). Major studies like the Human Genome Project (HGP), initiated in 1990 and completed in 2003, provided a starting point from which scientists could more thoroughly investigate the human condition on a genetic level. Arising from this study, personal genomics is considered a blooming field in genetics- in which rapidly developing technological advances are able to provide easier and …

The Role Of Cellulose Synthase-Like D Genes In Tip Growth Of Physcomitrella Patens, Erin E. Killeavy, Arielle Chaves, Alison Roberts

Senior Honors Projects

Physcomitrella patens is a non-vascular plant with a relatively small genome and is amongst the few eukaryotic organisms that have a high rate of homologous recombination. This is valuable in biological research because it allows for targeted genetic modification of the organism. In vascular plants like Arabidopsis thaliana, a model organism, Cellulose Synthase-like D (CSLD) genes have been discovered to be important in tip growth. This type of growth is observed in the pollen tubes and root hairs of these plant types. The CSLD genes in Arabidopsis were found to play a crucial role in the growth of …

Identification Of N-Acylethanolamine Hydrolyzing Enzyme In Solanum Lycopersicum, Derek A. Stuffle

Undergraduate Honors Theses

N-acylethanolamines (NAEs) are fatty acid derivatives that occur naturally in plant and animal systems. In mammals, they regulate physiological functions, including neurotransmission, immune responses, vasodilation, embryo development and implantation, feeding behavior, and cell proliferation. NAEs are metabolized by fatty acid amide hydrolase (FAAH), which belongs to the amidase signature family. It is hypothesized that putative FAAH functions as the catalyst in the metabolism of N-acylethanolamine in tomato plants. To test the hypothesis, FAAH protein homologs were identified in tomato via in silico analysis. Among the six homologs identified, FAAH1 and FAAH2 were selected for further validation. This study …

The Transfer Of Commensal And Transient Bacteria Into Deep Tissue Using Dry Needling Needles Through Agar Media Designed To Mimic The Elasticity Of Human Skin, Denisse N. Breton Guerrero

Honors Theses

Needle insertion into skin for the purpose of alleviating muscle pain began with the emergence of traditional Chinese medicinal (TCM) acupuncture. “Dry needling”, a recent alternative to acupuncture, involves inserting a monofilament needle down into the deep tissue of a patient’s skin in order to reach the affected muscle. Despite its effectiveness and low risk of infection, dry needling remains questionable with respect to its safety and the lack of research to confirm the low risk of bacterial transmission via the dry needling needles. In this microbiological study, highly concentrated Tryptic Soy Agar was utilized to simulate the elasticity of …

Alzheimer's Disease: The Silver Tsunami Of The 21st Century, Ankita Sarkar, Madison Irwin, Aditi Singh, Matthew Riccetti, Amit Singh

Biology Faculty Publications

Alzheimer's disease (AD), a fatal progressive neurodegenerative disorder, has no cure to date. One of the causes of AD is the accumulation of amyloid-beta 42 (Aß42) plaques, which result in the onset of neurodegeneration. It is not known how these plaques trigger the onset of neurodegeneration. There are several animal models developed to (i) study etiology of disease, (ii) look for genetic modifiers, and (iii) identify chemical inhibitors that can block neurodegeneration and help to find cure for this disease. An insect model of Drosophila melanogaster has also provided new insights into the disease. Here we will discuss the utility …

Tick-, Mosquito-, And Rodent-Borne Parasite Sampling Designs For The National Ecological Observatory Network, Yuri P. Springer, David Hoekman, Pieter T.J. Johnson, Paul A. Duffy, Rebecca A. Hufft, David T. Barnett, Brian F. Allan, Brian R. Amman, Christopher M. Barker, Roberto Barrera, Charles B. Beard, Lorenza Beati, Mike Begon, Mark S. Blackmore, William E. Bradshaw, Dustin Brisson, Charles H. Calisher, James E. Childs, Maria A. Diuk-Wasser, Richard J. Douglass, Rebecca J. Eisen, Desmond H. Foley, Janet E. Foley, Holly D. Gaff, Scott L. Gardner, Howard S. Ginsberg, Gregory E. Glass, Sarah A. Hamer, Mary H. Hayden, Brian Hjelle, Christina M. Holzapfel, Steven A. Juliano, Todd P. Livdahl

Biology

Parasites and pathogens are increasingly recognized as significant drivers of ecological and evolutionary change in natural ecosystems. Concurrently, transmission of infectious agents among human, livestock, and wildlife populations represents a growing threat to veterinary and human health. In light of these trends and the scarcity of long-term time series data on infection rates among vectors and reservoirs, the National Ecological Observatory Network (NEON) will collect measurements and samples of a suite of tick-, mosquito-, and rodent-borne parasites through a continental-scale surveillance program. Here, we describe the sampling designs for these efforts, highlighting sampling priorities, field and analytical methods, and the …

Population Dynamics Based On Resource Availability & Founding Effects: Live & Computational Models, Samuel Potter, Rebecca M. Krall, Susan Mayo, Diane Johnson, Kimberly J. Zeidler-Watters, Robin L. Cooper

Biology Faculty Publications

With the looming global population crisis, it is more important now than ever that students understand what factors influence population dynamics. We present three learning modules with authentic, student-centered investigations that explore rates of population growth and the importance of resources. These interdisciplinary modules integrate biology, mathematics, and computer-literacy concepts aligned with the Next Generation Science Standards. The activities are appropriate for middle and high school science classes and for introductory college-level biology courses. The modules incorporate experimentation, data collection and analysis, drawing conclusions, and application of studied principles to explore factors affecting population dynamics in fruit flies. The variables …

Transition Between Phases Of The Annual Cycle: Spring Migration To Breeding In Nearctic-Neotropical Songbirds, Kristen Marie Covino

Dissertations

Appropriate timing of each life-history stage is crucial for seasonally migratory species. The temporal constraints faced by migratory songbirds require that they overlap preparation for breeding with spring migration. However, previous work has focused primarily on male birds and has produced inconsistent results regarding the degree of overlap between these two life-history stages. I study the degree to which migrating male and female songbirds prepare for breeding throughout spring migration as they move towards their breeding grounds. Overall, male migrants show a significant degree of breeding preparation during spring migration as determined by circulating testosterone levels and their ability to …

The Role Of Orexin Receptors In Diurnal Variations In Learning And Memory, Jacob Christian Blotter

Undergraduate Honors Capstone Projects

The brain's ability to learn and remember is a topic of extensive debate and research. Mammals share many similarities, including the way in which information from the outside world is processed and stored. Mammalian circadian rhythms have long been thought to be essentially involved with these processes. Orexin, a neurotransmitter in the brain, plays a crucial role in arousal and circadian rhythm. This study is designed to explore the brain's ability to process and store information at different times of the circadian period, and to explore the role of orexin by comparing the performance of normal (wild-type) mice and abnormal …

A National Strategy For The Conservation Of Native Freshwater Mollusks, Megan Bradley, Robert S. Butler, Heidi L. Dunn, Catherine Gatenby, Patricia A. Morrison, Teresa J. Newton, Matthew Patterson, Kathryn E. Perez

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

In 1998, a strategy document outlining the most pressing issues facing the conservation of freshwater mussels was published (NNMCC 1998). Beginning in 2011, the Freshwater Mollusk Conservation Society began updating that strategy, including broadening the scope to include freshwater snails. Although both strategy documents contained 10 issues that were deemed priorities for mollusk conservation, the identity of these issues has changed. For example, some issues (e.g., controlling dreissenid mussels, technology to propagate and reintroduce mussels, techniques to translocate adult mussels) were identified in the 1998 strategy, but are less prominent in the revised strategy, due to changing priorities and progress …

Effects Of Arachidin-1 And Arachidin-3 On Human Colon Cancer Cells, Jessica L. Bettis

Biological Sciences Undergraduate Honors Theses

Cancer remains a leading cause of death in the state of Arkansas and across the United States. Recent research found that peanuts consumption has been correlated with a decreased risk of cancer. Although limited research has been conducted, phenolic antioxidant compounds found in peanuts, arachidin-1 and arachidin-3, are correlated to a decrease in human leukemia and lung cancer cell growth. The objective of this study was to evaluate the anti-colon cancer effects of these compounds. Experiments were conducted to examine the effects of purified arachidin-1 and arachidin-3 on Caco-2 cell proliferation. Treatment of cells with arachidin-1 (0, 1, 5, 10µM) …

Response Of Leukocyte Profiles To Corticosterone Manipulation In The Prairie Rattlesnake (Crotalus Viridus), Jill E. Guillette

Biological Sciences Undergraduate Honors Theses

Prior work has shown that when animals are stressed they have altered leukocyte profiles. The use of leukocyte profiles could provide an inexpensive and efficient method for determining stress. Here we present a validation of the use of leukocyte profiles for showing induced stress in Prairie Rattlesnakes (Crotalus viridus). Snakes (N=19) with masses ranging from 682g to 137g were used in a repeated measures design to examine the effect of hormone manipulation on leukocyte profiles. During each trial snakes were dosed with either corticosterone and sesame oil (1.1µg/g) or only sesame oil. Prior to dosing, baseline blood samples …

Trinucleotide Heterogeneity Expansions: In Vitro Molecule Amplification & Purification, Gennaro Dellicarpini

Biological Sciences

Simple sequence repeats (SSRs) are mutation-prone DNA tracts composed of tandem repetitions of relatively short motifs (Kashi & King, 2006). SSRs are found scattered throughout the human genome in both coding and non-coding regions. Present under the category of SSRs are Trinucleotide repeats, which may vary in number and, when expanded upon, provide the basis of at least nine neurodegenerative diseases. Large degrees of structural heterogeneity are found in CAG trinucleotide repeat DNA. DNA slippage leads these hetero-structures to form, since certain repeats slip out of the origin of replication and are carried into the next generation of replication. This …