Biology Commons^™

Bioregulatory Systems Medicine: An Innovative Approach To Integrating The Science Of Molecular Networks, Inflammation, And Systems Biology With The Patient's Autoregulatory Capacity?, Alyssa W Goldman, Yvonne Burmeister, Konstantin Cesnulevicius, Martha Herbert, Mary Kane, David Lescheid, Timothy Mccaffrey, Myron Schultz, Bernd Seilheimer, Alta Smit, Georges St Laurent, Brian Berman

Medicine Faculty Publications

Bioregulatory systems medicine (BrSM) is a paradigm that aims to advance current medical practices. The basic scientific and clinical tenets of this approach embrace an interconnected picture of human health, supported largely by recent advances in systems biology and genomics, and focus on the implications of multi-scale interconnectivity for improving therapeutic approaches to disease. This article introduces the formal incorporation of these scientific and clinical elements into a cohesive theoretical model of the BrSM approach. The authors review this integrated body of knowledge and discuss how the emergent conceptual model offers the medical field a new avenue for extending the …

Biological Sciences Newsletter - August 2015, Department Of Biological Sciences, East Tennessee State University

Biological Sciences Newsletters

No abstract provided.

Virulence Evolution Of Fungal Pathogens In Social And Solitary Bees With An Emphasis On Multiple Infections, Ellen G. Klinger

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The health of pollinators, especially bees, is of the utmost importance to success of many agricultural ecosystems. Microorganisms can cause diseases in bees; such microbes are pathogenic. The ability of a pathogen to cause harm to its host (such as a bee) is termed its virulence. Studying the evolution of different levels of virulence can lead researchers to a better understanding of pathogens, and potentially predict how much harm a pathogen can cause in the future. We studied the evolution of virulence levels for a fungal disease of bees. This group of fungi is composed of 28 species, and some …

A Dual Function Of White Coloration In A Nocturnal Spider Dolomedes Raptor (Araneae: Pisauridae), Tai-Shen Lin, Shichang Zhang, Chen-Pan Liao, Eileen A. Hebets, I-Min Tso

Eileen Hebets Publications

Nocturnal animals frequently possess seemingly conspicuous color patterns that can function in a variety of ways (e.g. prey attraction, camouflage, predator avoidance, etc.). The use of color patterns in intraspecific signaling, especially reproductive activities, in nocturnal animals has received relatively little attention. This study tested for a dual function of color in the nocturnal fishing spider, Dolomedes raptor (Araneae: Pisauridae), whose males develop dimorphic white stripes at sexual maturation. We tested for a role in foraging as well as mate assessment. First, quantifications of the natural variation of male stripes indicated a correlation between stripe area and male body size …

An Investigation Of The Effect Of Malathion On Adaptive Plasticity Of Pseudacris Sierra, Michael Jonathan Maples

Master's Theses

This thesis is composed of two chapters. Chapter one reviews what is known about adaptive plasticity in response to predators, describes the physiological systems involved in such plasticity, and outlines the evolutionary consequences of adaptive plasticity. Chapter two describes a scientific experiment that investigates how malathion may impact adaptive plasticity in the Sierran Treefrog, Pseudacris sierra. Anuran tadpoles suffer high mortality rates due to predation. In response to strong selective forces relating to these high predation rates, tadpoles evolved the ability to adaptively respond to predators through morphological and behavioral plasticity. The morphological and behavioral responses are varied and depend …

Microbial Source Tracking Of Nonpoint Source Pollution In New Jersey Rivers, Matthew John Newton

Theses, Dissertations and Culminating Projects

The bodies of water of New Jersey serve many different purposes from recreational to agricultural and drinking to waste water treatment. Due to the vast diversity of usage and the many people that rely on these water bodies for everyday life it is essential that the health and quality of the water bodies is monitored and maintained regularly. Sources of pollution that affect the health of rivers, lakes and streams include biological, microbial, physical and chemical contaminants. All of these pollutions can impact the health of the water body, the organisms living in it and those who come into contact …

Prevalence Of A Chytrid Pathogen (Batrachochytrium Dendrobatidis) In Eastern Hellbender Salamanders In New York And Pennsylvania, Linxuan Wu

Biology Theses

Amphibian populations are currently declining globally. There are many possible causes for these declines, among which an emerging infectious disease, chytridiomycosis, has been implicated. Chytridiomycosis in the U.S.A. is mainly caused by the Batrachochytrium dendrobatidis. In this study, I used qPCR assays to detect the existence of this pathogen in the Eastern Hellbender (Cryptobranchus alleganiensis alleganiensis) populations in the Allegheny and Susquehanna River drainages of New York and Pennsylvania. Chytrid is most often tested by using skin swabs, but in this study, tail clips, dorsal skin, blood and eggs were tested as well. Batrachochytrium dendrobatidis was detected …

The Sea Lamprey Meiotic Map Improves Resolution Of Ancient Vertebrate Genome Duplications, Jeramiah James Smith, Melissa C. Keinath

Biology Faculty Publications

It is generally accepted that many genes present in vertebrate genomes owe their origin to two whole-genome duplications that occurred deep in the ancestry of the vertebrate lineage. However, details regarding the timing and outcome of these duplications are not well resolved. We present high-density meiotic and comparative genomic maps for the sea lamprey (Petromyzon marinus), a representative of an ancient lineage that diverged from all other vertebrates ~550 million years ago. Linkage analyses yielded a total of 95 linkage groups, similar to the estimated number of germline chromosomes (1n ~ 99), spanning a total of 5570.25 cM. …

Identification And Abundance Of Jellyfish In The San Francisco Estuary, Carrie Fong

STAR Program Research Presentations

In 2010 the delta smelt (Hypomesus transpacificus), became protected under the California Endangered Species Act. Endemic to the San Francisco Estuary, their numbers have been declining since the mid 1990s. Many factors have contributed to their low numbers, such as alterations to their habitat, predation, water diversions, and prey abundance. Several invasive species of jellyfish may be sources of competition for the native delta smelt because they both eat the same food source of copepods. From July 2010 to December 2012, samples of zooplankton, including jellyfish, were taken from 9 different stations in the high and low salinity …

A Study Of Ret Dependent Transcriptional Changes Of Cell Signaling Proteins In Neuroblastoma Cells, Elizabeth Yvonne Flores

Theses, Dissertations and Culminating Projects

The ret proto-oncogene codes for a receptor tyrosine kinase involved in the pathogenesis of numerous developmental defects, particularly those in neural crest-derived structures leading to the regulation of cell proliferation, migration, differentiation and survival during embryogenesis. Constitutive activation or inactivation of the RET receptor results in the cancer syndrome Multiple Endocrine Neoplasia or Hirschsprung's disease, respectively. The importance of RET in these disease states has been well established. However, while many proteins associated with the RET signaling pathway have been identified, the transcriptional changes induced by the wild type receptor, particularly with respect to these signaling proteins in neuroblastoma cells, …

The Effects Of Crude And Pure Green Tea Polyphenols On Bacteria Biofilm Formation And Endospore Germination, Hassan Tahir

Theses, Dissertations and Culminating Projects

Over the past couple of decades humans have been combating the problem of multi-drug resistant microorganisms. Multi-drug resistant strains o f microorganisms have been causing not only problems for medical settings, but the world as well. Because of the continuous use of antibiotics within our time and age, there has been a huge increase in antibiotic resistant bacteria. In addition to the rise in multi-drug resistant bacteria over time, endospore cells have been causing increased levels of food-bourne illnesses. Thus, there is a need for novel antimicrobial agents from nature derived products as an alternative way to treat and control …

Home Range, Habitat Use, And Movements Of Native Northern Map Turtles (Graptemys Geographica), And Sympatric Invasive Red-Eared Slider Turtles (Trachemys Scripta Elegans), In The Upper Niagara River, Brian E. Haas

Biology Theses

Turtle populations throughout the world are in decline due to the effects associated with anthropogenic disturbances. Northern map turtles in the Upper Niagara River are facing the same effects associated with shoreline development, pollution, and human induced mortality. A biotelemetric study was conducted to understand the population structure, habitat use, and behavior of northern map turtles in the Upper Niagara River. Turtles were trapped, outfitted with radio and sonic transmitters, and tracked from August 2013 until April 2015. Invasive red-eared sliders, which also inhabit the Niagara River were captured and tracked to allow for comparison. This invasive species is a …

Distinct Physiological Roles For The Two Isoforms Of The Er Chaperone Grp170 In Caenorhabditis Elegans, Yuanyuan Li

Biology Theses

GRP170 is a large molecular chaperone found in the ER of all eukaryotes. The nematode Caenorhabditis elegans has two loci encoding GRP170: T24H7.2 (grp170a) and T14G8.3 (grp170b). The phenotypes of nematodes genetically deficient for either grp170a or grp170b were compared to a standard laboratory strain with functional grp170 loci. Worms that were deficient for grp170a developed 32% slower than the control strain. The loss of grp170a had a significant but modest reduction on the life span compared to the control strain. Worms deficient for grp170a also displayed significantly increased embryonic lethality and resulted in 6.9% arrested embryos. The loss of …

Participant Perceptions Of Range Rider Programs Used To Mitigate Wolf-Livestock Conflicts In The Western United States, Molly Parks

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Range Rider Programs (RRPs) are one example of a proactive non-lethal tool that has been implemented in western United States to mitigate gray wolf (Canis lupus) and livestock conflicts. Because RRPs are an emerging non-lethal tool that little is known about, I selected a qualitative research approach to examine participant perceptions to further contemporary understanding of how these efforts are implemented and potential benefits. I surveyed 51 participants from 17 Range Rider Programs (RRPs) in Montana, Washington, and Oregon to determine participant perceptions regarding effectiveness of RRPs as a non-lethal approach to mitigate wolf-conflicts.

I developed a RRPs typology based …

Evaluating Accuracy And Bias In Genetic Monitoring Under Scenarios Of Population Decline, Susan K. Munster

Masters Theses

Accurate and unbiased estimates of current effective population size are of primary importance in making informed decisions for conservation purposes. One species of concern, the eastern red bat (Lasiurus borealis), is experiencing ongoing population losses due to wind turbine collisions. The proportion of the population being affected is currently unknown, although recent estimates of total fatalities at wind turbines over the time from 2000-2011 are in the hundreds of thousands for this species alone. The roosting habits of eastern red bats make it difficult to monitor their census population size (Nc); thus, genetic estimates of effective population size (Ne) may …

Interkingdom Communication: Study Of Caenorhabditis Elegans And Vibrio Cholerae Interactions, Joseph Angeloni

All Theses

Vibrio cholerae is a Gram-negative, rod-shaped bacterium that is mainly found in water environments, and is the causative agent of the disease cholera in humans. It is critical for this bacterium to communicate via quorum sensing to persist and survive in the environment, as well as cause infection. Recently, it has been shown that eukaryotes are able to sense and respond to certain quorum sensing molecules, known as autoinducers, which are produced by different bacteria. Caenorhabditis elegans is a particularly useful model for studying this interaction. During preliminary experiments, it was noticed that C. elegans were strongly attracted to V. …

Assessing The Impacts Of Macroalgal Competition And Parrotfish Grazing On Coral Cover In The Middle Florida Keys, Kylie Smith

All Theses

The coral reefs of the Florida Keys National Marine Sanctuary (FKNMS) are diverse ecosystems that support thousands of marine organisms. Unfortunately, the live coral cover on these reefs has dramatically decreased over the last 30 years even in the most protected areas of the sanctuary. Environmental changes have contributed to this decline in coral cover by creating optimal conditions for macroalgal growth. These macroalgae species are fast growing and out-compete slow growing corals for space and nutrients. Herbivores known to forage on macroalgae, including long-spined sea urchins and parrotfishes, have decreased in abundance. A synthesis of data from across the …

Environmental Effects On Fish Escape Responses: Impact Of Flow On The Escape Performance Of The Hawaiian Stream Goby, Sicyopterus Stimpsoni, Kelly Diamond

All Theses

The ability of fish to escape from predators is a critical behavior for their survival. Experimental measurements of escape performance in fishes have typically been based on trials conducted in still water; however, such environmental conditions are rare in nature due to waves and currents that expose fishes to unsteady and/or directional flow. I examined the effects of water flow and predator attack direction on the escape behavior of fish, using juveniles of the amphidromous Hawaiian goby Sicyopterus stimpsoni as a model system. In nature, these fish must escape ambush predation by the Hawaiian sleeper, Eleotris sandwicensis, while exposed …

Assessing Biogeochemical Impacts And Environmental Conditions Associated With Cross-Shelf High Chlorophyll Plumes In The Northern Gulf Of Mexico, Erin Brooke Jones

Dissertations

The northern Gulf of Mexico is a complex marine system subject to episodic physical phenomena such as loop current eddies. Flow fields generated by these eddies can result in cross-shelf exchanges between riverine influenced shelf waters and the offshore water column. This study considers the impacts of high chlorophyll plumes (HCPs) resulting from cross-shelf exchanges to the bio-optical properties of affected waters and how these plumes are influenced by their environment. The seasonal, interannual and decadal chlorophyll cycles of the Gulf of Mexico and the northern Gulf of Mexico are described to provide context for evaluating the ecological effects of …

Microcystin Accumulation In Fish Muscle Tissue: Exploring The Safety Of Fish Consumption In Several Michigan Water Bodies, Heather Snyder

Masters Theses

A variety of anthropogenic factors, including global climate change and eutrophication, are causing increases in cyanobacterial abundance. Increased prevalence of cyanobacteria can be detrimental, as some genera of cyanobacteria have the ability to produce toxic secondary metabolites known as cyanotoxins. The cyanotoxin microcystin- a hepatotoxin, is the most ubiquitous and toxic cyanotoxin in freshwater ecosystems, and has been quantified in a number of aquatic organisms. Microcystin was quantified in 8 fish species from St. Mary’s River in June, Saginaw Bay in September, and from Stony Creek Lake in October in 2014 by the Michigan Department of Environmental Quality and the …

Toxin Production And Population Dynamics Of Gloeotrichia Echinulata With Considerations Of Global Climate Change, Sarah Stamann

Masters Theses

Global climate change has been identified as a driver for increasing cyanobacteria blooms world-wide. Blooms of the cyanobacterium, Gloeotrichia echinulata, (hereafter, G. echinulata) have been observed in Silver Lake (Oceana County, MI), often forming dense surface scums. This organism is known to produce the hepatotoxin, microcystin- LR, and its growth is linked to phosphorus accumulation from sediment and temperature.

A series of experiments at ambient and elevated temperatures (+2° and +6°above ambient) with sediment from four locations in Silver Lake with varying phosphorus concentrations were conducted to examine the effect of these variables on G. echinulata growth. These …

Ecology And Morphology Of The Late Miocene Musk Deer, Longirostromeryx Wellsi (Artiodactyla: Moschidae: Blastomerycinae), Katheryn Y. C. Chen

Department of Earth and Atmospheric Sciences: Dissertations, Theses, and Student Research

Longirostromeryx wellsi, one of the latest surviving members of the extinct clade Blastomerycinae (Artiodactyla: Moschidae), possesses highly derived craniodental morphology that deviates from typical musk deer form. Previous work suggests that the unique anatomy of L. wellsi represents adaptations for occupying open savannas. To test this hypothesis I conduct principal components analysis on five postcranial bones of L. wellsi, comparing them to that of several extant ruminant artiodactyls, which are divided among seven habitat categories. These elements are also compared with the postcrania of other blastomerycines. These analyses indicate that L. wellsi anatomy is most similar to that of other …

Beyond Bivariate Correlations: Three-Block Partial Least Squares Illustrated With Vegetation, Soil, And Topography, Daehyun Kim, Thomas J. Dewitt, César S. B. Costa, John A. Kupfer, Ryan W. Mcewan, J. Anthony Stallins

Biology Faculty Publications

Ecologists, particularly those engaged in biogeomorphic studies, often seek to connect data from three or more domains. Using three-block partial least squares regression, we present a procedure to quantify and define bi-variance and tri-variance of data blocks related to plant communities, their soil parameters, and topography. Bi-variance indicates the total amount of covariation between these three domains taken in pairs, whereas tri-variance refers to the common variance shared by all domains. We characterized relationships among three domains (plant communities, soil properties, topography) for a salt marsh, four coastal dunes, and two temperate forests spanning several regions in the world. We …

Canonical Bmp Signaling Is Required For Allodynia In Drosophila Melanogaster, Taylor L. Follansbee

All Theses And Dissertations

In the United States alone over 100 million people suffer from chronic pain and unfortunately, even still, there is a lack in scientific understanding for the mechanisms of abnormal pain sensitivity. The present study utilized a candidate gene approach to identify novel components required for modulation of the tissue damage induced pain sensitization pathway in Drosophila melanogaster. We have shown that RNAi silencing of decapentaplegic (dpp), a member of the Bone Morphogenetic Protein (BMP) signaling pathway, specifically in the class IV multidendritic nociceptor neurons significantly attenuated UV-induced nociceptive sensitization. Furthermore, overexpression of dpp in nociceptor neurons was sufficient to …

Two Cryptic Species Within Astragalus Cusickii Delimited Using Molecular Phylogenetic Techniques, Jay Christopher Zimmers

Boise State University Theses and Dissertations

Understanding the source of phenotypic variability is a challenge in the biological sciences. Variation in phenotypes is the result of variation in the genetics and environment the organism experiences, but elucidating the relative contribution of these two parameters can pose problems, especially in the field of systematics. Systematists are challenged to classify biological diversity into groups that share common ancestry. Phenotypic variation can be useful to demonstrate common ancestry, but only when the primary contributor to the variation is under strong genetic control, and thus heritable. Cusick’s milkvetch (Astragalus cusickii) is a perennial forb endemic to the intermountain …

An Analysis Of The Differential Methylation And Expression Of Imprinted Genes In M. M. Musculus, M. M. Domesticus, And Their Hybrids, Anna P. Rice

All NMU Master's Theses

Epigenetics has been found to have an effect on many aspects of biology. Epigenetics refers to modifications of the double-stranded DNA molecule, which do not change the nucleotide sequence but do affect gene expression. DNA methylation is a type of epigenetic modification. Genomic imprinting is a pattern of gene expression that is primarily achieved through DNA methylation, and it results in the expression of only one allele at a particular locus. In this study, I analyzed the methylation patterns of five imprinted genes in the hybrids of two different lab strains of the house mouse subspecies, M. m. musculus and …

Assessment Of Behavior, Home Range, Population Size, And Intermixing Among The Atlantic Bottlenose Dolphin, Tursiops Truncatus, In New Jersey, Colleen Marie Talty

Theses, Dissertations and Culminating Projects

Bottlenose dolphins (Tursiops truncatus) are known to exhibit a range of different social structures and habitat use profiles. To investigate the structure of the population off Cape May, NJ, photo identification techniques were employed. Between April 2013 and November 2014, 209 survey days with 989 individual sightings were observed from a platform of opportunity, the whale watching vessel the American Star. The primary goals of this study were to assess the population size in this area; determine whether or not the inshore ecotype were mixing with the offshore ecotype; determine home range size for dolphins sighted multiple times; and to …

Investigations Of Filarial Nematode Motility, Response To Drug Treatment, And Pathology, Charles Nutting

Dissertations

More than a billion people live at risk of chronic diseases caused by parasitic filarial nematodes. These diseases: lymphatic filariasis, onchocerciasis, and loaisis cause significant morbidity, degrading the health, quality of life, and economic productivity of those who suffer from them. Though treatable, there is no cure to rid those infected of adult parasites. The parasites can modulate the immune system and live for 10-15 years. Testing of compounds against filarial nematodes is complicated due to a lack of an objective platform on which to analyze in vitro treatments. There is no published, immunocompetent laboratory model for lymphatic filariasis. This …

Spatiotemporal Dynamics Of Coarse Woody Debris In An Old-Growth Temperate Deciduous Forest, Jessica G. Davis, Julia I. Chapman, Shuang-Ye Wu, Ryan W. Mcewan

Biology Faculty Publications

No abstract provided.

Water Transport And The Functional Dynamics Of Aquaporins In Osmoregulatory Organs Of Fishes, Steffen S. Madsen, Morton B. Engelund, Christopher P. Cutler

Biology: Faculty Publications

Aquaporins play distinct roles for water transport in fishes as they do in mammals—both at the cellular, organ, and organismal levels. However, with over 32,000 known species of fishes inhabiting almost every aquatic environment, from tidal pools, small mountain streams, to the oceans and extreme salty desert lakes, the challenge to obtain consensus as well as specific knowledge about aquaporin physiology in these vertebrate clades is overwhelming. Because the integumental surfaces of these animals are in intimate contact with the surrounding milieu, passive water loss and uptake represent two of the major osmoregulatory challenges that need compensation. However, neither obligatory …