Biology Commons^™

Consequences Of The Historical Demography On The Global Population Structure Of Two Highly Migratory Cosmopolitan Marine Fishes: The Yellowfin Tuna (Thunnus Albacares) And The Skipjack Tuna (Katsuwonus Pelamis), Bert Ely, Jordi Viñas, Jaime R. Alvarado Bremer, Donna Black, Luciano Lucas, Kelly Covello, Alexis V. Labrie, Eric Thelen

Faculty Publications

Background

Yellowfin and skipjack tuna are globally distributed in the world's tropical and sub-tropical oceans. Since little, if any, migration of these fishes occurs between the Atlantic and Indo-Pacific Oceans, one might expect to see genetic differences between sub-populations in these ocean basins. However, yellowfin and skipjack tuna have extremely large population sizes. Thus, the rate of genetic drift should be slower than that observed for other tunas.

Results

Low levels of genetic differentiation were observed between Atlantic and Pacific samples of yellowfin tuna. In contrast, no genetic differentiation was observed between Atlantic and Pacific samples of skipjack tuna.

Conclusion …

From The Cover: Assignment Of An Essential Role For The Neurospora Frequency Gene In Circadian Entrainment To Temperature Cycles, Antonio M. Pregueiro, Nathan Price-Lloyd, Deborah Bell-Pedersen, Christian Heintzen, Jennifer J. Loros, Jay C. Dunlap

Dartmouth Scholarship

Circadian systems include slave oscillators and central pacemakers, and the cores of eukaryotic circadian clocks described to date are composed of transcription and translation feedback loops (TTFLs). In the model system Neurospora, normal circadian rhythmicity requires a TTFL in which a White Collar complex (WCC) activates expression of the frequency (frq) gene, and the FRQ protein feeds back to attenuate that activation. To further test the centrality of this TTFL to the circadian mechanism in Neurospora, we used low-amplitude temperature cycles to compare WT and frq-null strains under conditions in which a banding rhythm was elicited. WT cultures were entrained …

Two Crystal Structures Of Dihydrofolate Reductase-Thymidylate Synthase From Cryptosporidium Hominis Reveal Protein–Ligand Interactions Including A Structural Basis For Observed Antifolate Resistance, Amy C. Anderson

Dartmouth Scholarship

Cryptosporidium hominis is a protozoan parasite that causes acute gastro- intestinal illness. There are no effective therapies for cryptosporidiosis, highlighting the need for new drug-lead discovery. An analysis of the protein ligand interactions in two crystal structures of dihydrofolate reductase- thymidylate synthase 􏰀DHFR-TS) from C. hominis, determined at 2.8 and 2.87 AÊ resolution, reveals that the interactions of residues Ile29, Thr58 and Cys113 in the active site of C. hominis DHFR provide a possible structural basis for the observed antifolate resistance. A comparison with the structure of human DHFR reveals active-site differences that may be exploited for the design of …

Relationship Among Genes Conferring Partial Resistance To Leaf Rust (Puccinia Triticina) In Wheat Lines Ci 13227 And L-574-1., Jeffery S. Lehman, Karin A. Hanson, Gregory Shaner

Biology and Earth Science Faculty Scholarship

This study describes the segregation of genes for resistance to the fungus Puccinia triticinain a cross between partially resistant wheat lines L-574-1 and CI 13227 with two and four genes for resistance, respectively. The objectives of this study were to use parental, F1, F2, and backcross populations to quantify maternal effects, degree of dominance, and transgressive segregation, and to determine whether CI 13227 and L-574-1 share any resistance genes for long latent period or small uredinia. In two experiments conducted in the greenhouse, the uppermost leaf of adult wheat plants was inoculated prior to heading with P. triticina. On days …

Opinion: Avian Brains And A New Understanding Of Vertebrate Brain Evolution, Gerald E. Hough, The Avian Brain Nomenclature Consortium

College of Science & Mathematics Departmental Research

We believe that names have a powerful influence on the experiments we do and the way in which we think. For this reason, and in the light of new evidence about the function and evolution of the vertebrate brain, an international consortium of neuroscientists has reconsidered the traditional, 100-year-old terminology that is used to describe the avian cerebrum. Our current understanding of the avian brain — in particular the neocortex-like cognitive functions of the avian pallium — requires a new terminology that better reflects these functions and the homologies between avian and mammalian brains.

Hybrid Xerogel Films As Novel Coatings For Antifouling And Fouling Release, Ying Tang, John A. Finlay, Gregory L. Kowalke, Anne E. Meyer, Frank V. Bright, Maureen E. Callow, James A. Callow, Dean E. Wendt, Michael R. Detty

Biological Sciences

Hybrid sol-gel-derived xerogel films prepared from 45/55 (mol ratio) n-propyltrimethoxysilane (C3-TMOS)/tetramethylorthosilane (TMOS), 2/98 (mol ratio) bis[3-(trimethoxysilyl)propyl]-ethylenediamine (enTMOS)/tetraethylorthosilane (TEOS), 50/50 (mol ratio) n-octyltriethoxysilane (C8-TEOS)/TMOS, and 50/50 (mol ratio) 3,3,3-trifluoropropyltrimethoxysilane (TFP-TMOS)/TMOS were found to inhibit settlement of zoospores of the marine fouling alga Ulva (syn. Enteromorpha) relative to settlement on acid-washed glass and give greater release of settled zoospores relative to glass upon exposure to pressure from a water jet. The more hydrophobic 50/50 C8-TEOS/TMOS xerogel films had the lowest critical surface tension by comprehensive contact angle analysis and gave significantly greater release of 8-day Ulva sporeling biomass after exposure to …

Structure Of The P115rhogef Rgrgs Domain-Galpha13/I1 Chimera Complex Suggests Convergent Evolution Of A Gtpase Activator, Zhe Chen, William D. Singer, Paul C. Sternweis, Stephen R. Sprang

Biological Sciences Faculty Publications

p115RhoGEF, a guanine nucleotide exchange factor (GEF) for Rho GTPase, is also a GTPase-activating protein (GAP) for G12 and G13 heterotrimeric Galpha subunits. The GAP function of p115RhoGEF resides within the N-terminal region of p115RhoGEF (the rgRGS domain), which includes a module that is structurally similar to RGS (regulators of G-protein signaling) domains. We present here the crystal structure of the rgRGS domain of p115RhoGEF in complex with a chimera of Galpha13 and Galphai1. Two distinct surfaces of rgRGS interact with Galpha. The N-terminal betaN-alphaN hairpin of rgRGS, rather than its RGS module, forms intimate contacts with the catalytic site …

Connecting Branches To Student Activities, Amy Cheng Vollmer

Biology Faculty Works

No abstract provided.

Mechanisms For The Environmental Regulation Of Gene Expression: Ecological Aspects Of Animal Development, Scott F. Gilbert

Biology Faculty Works

The environment can play a significant role in the production of phenotypes. However, the developmental mechanisms by which the environmental agents effect normal development are Just becoming known. At least three paths have been found through which the environment can modify gene activity. The first is the neuroendocrine route. Here, the nervous system monitors the environment and transfers signals to the endocrine system. The endocrine hormones can then alter gene expression. The second route involves environmental factors that change the methylation pattern of genes, thereby altering their transcriptional capabilities. The third route involves the direct induction of gene expression in …

Assessment Of Biogeochemical Deposits In Landfill Drainage Systems, Audrey D. Levine, Valerie J. Harwood, Antonio J. Cardoso, Lisa R. Rhea, Bina S. Nayak, Barbara M. Dodge, Mindy L. Decker, George Dzama, Lawrence Jones, Edward Haller

Integrative Biology Faculty and Staff Publications

Design, operation, and closure practices for Class I municipal solid waste (MSW) landfills are based on RCRA Subtitle D requirements for control of leachates and gases generated during the life of the landfill. Because leachate is generated as a result of water percolating through the landfill and waste consolidation, it contains dissolved and suspended materials that reflect the characteristics of the material it contacts. Over the past two decades significant changes have occurred in the characteristics of the waste streams that are disposed in landfills. In addition, many Florida municipalities have implemented Waste-to-Energy (WTE) systems. The WTE ash is either …

Phylogenetic Reconstruction Of Exoristinae Using Molecular Data: A Bayesian Re-Analysis, John O. Stireman Iii

Biological Sciences Faculty Publications

A few years ago I published the results of a phylogenetic analysis of New World Exoristinae based on molecular data from two genes, 28S rDNA and Elongation Factor 1-alpha (Stireman 2002). In that study I employed parsimony, neighbor joining, and maximum likelihood inference methods to generate phylogenetic reconstructions, and explored a variety of weighting schemes and combinations of the sequence data (i.e. each gene separately and both together). The results of these analyses generally supported recent taxonomic hypotheses (e.g., Herting 1984; Wood 1987; O’Hara and Wood 2004). For example, Tachinidae and Exoristinae were reconstructed as monophyletic in most analyses, as …

Functional Analysis Of Subunit E Of The F1FO-Atp Synthase Of The Yeast Saccharomyces Cerevisiae: Importance Of The N-Terminal Membrane Anchor Region, Valerie Everard-Gigot, Cory D. Dunn, Brigid M. Dolan, Susanne Brunner, Robert E. Jensen, Rosemary A. Stuart

Biological Sciences Faculty Research and Publications

Mitochondrial F₁F_o-ATP synthase complexes do not exist as physically independent entities but rather form dimeric and possibly oligomeric complexes in the inner mitochondrial membrane. Stable dimerization of two F₁F_o-monomeric complexes involves the physical association of two membrane-embedded F_o-sectors. Previously, formation of the ATP synthase dimeric-oligomeric network was demonstrated to play a critical role in modulating the morphology of the mitochondrial inner membrane. In Saccharomyces cerevisiae, subunit e (Su e) of the F_o-sector plays a central role in supporting ATP synthase dimerization. The Su e protein is anchored …

Development And Validation Of A Food-Frequency Questionnaire For The Determination Of Detailed Fatty Acid Intakes, Maria Cantwell, Michael Gibney, Denis Cronin, Katherine Younger, John O'Neill, Linda Hogan, Mary Flynn

Articles

Objective: To validate a fat intake questionnaire (FIQ) developed to assess habitual dietary intake while focusing on the assessment of detailed fatty acid intake including total trans unsaturated fatty acids (TUFA). Design: An 88 food item/food group FIQ was developed using a meal pattern technique. Validation was achieved by comparison with dietary intake assessed by a modified diet history (DH) in a cross-over design. Eighty-four individuals supplied adipose tissue biopsies for linoleic acid and total TUFA analysis as an independent validation of the FIQ and DH. Setting: Medical Centre, Dublin Airport, Republic of Ireland. Subjects: One hundred and five healthy …

Dimeric Novel Hsp40 Is Incorporated Into The Radial Spoke Complex During The Assembly Process In Flagella, Chun Yang, Mark M. Compton, Pinfen Yang

Biological Sciences Faculty Research and Publications

The radial spoke is a stable structural complex in the 9 + 2 axoneme for the control of flagellar motility. However, the spokes in Chlamydomonas mutant pf24 are heterogeneous and unstable, whereas several spoke proteins are reduced differentially. To elucidate the defective mechanism, we clone RSP16, a prominent spoke protein diminished in pf24 axonemes. Unexpectedly, RSP16 is a novel HSP40 member of the DnaJ superfamily that assists chaperones in various protein-folding-related processes. Importantly, RSP16 is uniquely excluded from the 12S spoke precursor complex that is packaged in the cell body and transported toward the flagellar tip to be …

Elimination Of Foreign Dna During Somatic Differentiation In Tetrahymena Thermophila Shows Position Effect And Is Dosage Dependent, Yifan Liu, Xiaoyuan Song, Martin A. Gorovsky, Kathleen M. Karrer

Biological Sciences Faculty Research and Publications

In the ciliate Tetrahymena thermophila, approximately 15% of the germ line micronuclear DNA sequences are eliminated during formation of the somatic macronucleus. The vast majority of the internal eliminated sequences (IESs) are repeated in the micronuclear genome, and several of them resemble transposable elements. Thus, it has been suggested that DNA elimination evolved as a means for removing invading DNAs. In the present study, bacterial neo genes introduced into the germ line micronuclei were eliminated from the somatic genome. The efficiency of elimination from two different loci increased dramatically with the copy number of the neo genes in the …

Ua1b1/7 Complex For Engineering & Biological Sciences Dedication, Western Kentucky University

WKU Administration Documents

Program for the Complex for Engineering & Biological Sciences dedication, includes image of the building and floor plans.

The Genetics Of Ray Pattern Variation In Caenorhabditis Briggsae, Scott Everet Baird, Cynthia R. Davidson, Justin C. Bohrer

Biological Sciences Faculty Publications

Background: How does intraspecific variation relate to macroevolutionary change in morphology? This question can be addressed in species in which derived characters are present but not fixed. In rhabditid nematodes, the arrangement of the nine bilateral pairs of peripheral sense organs (rays) in tails of males is often the most highly divergent character between species. The development of ray pattern involves inputs from hometic gene expression patterns, TGFβ signalling, Wnt signalling, and other genetic pathways. In Caenorhabditis briggsae, strain-specific variation in ray pattern has provided an entrée into the evolution of ray pattern. Some strains were fixed for a derived …

Correlational Selection Leads To Genetic Integration Of Body Size And An Attractive Plumage Trait In Dark-Eyed Juncos, Joel Mcglothlin, Patricia Parker, Val Nolan, Ellen Ketterson

Biology Department Faculty Works

When a trait's effect on fitness depends on its interaction with other traits, the resultant selection is correlational and may lead to the integration of functionally related traits. In relation to sexual selection, when an ornamental trait interacts with phenotypic quality to determine mating success, correlational sexual selection should generate genetic correlations between the ornament and quality, leading to the evolution of honest signals. Despite its potential importance in the evolution of signal honesty, correlational sexual selection has rarely been measured in natural populations. In the dark‐eyed junco (Junco hyemalis), males with experimentally elevated values of a plumage trait (whiteness …

Effects Of Food Deprivation On Blood Lipid Concentration And Composition In Steller Sea Lions (Eumetopias Jubatus), Michelle Lea Berman

Electronic Theses and Dissertations

Steller sea lions, the largest Otariid, fast during their breeding season; during this time they refrain from ingesting food for a period of 12-43 days. Fasting, while undertaking an extremely energetically demanding activity (breeding and pupping), requires specific physiological adaptations. This study examined the physiological response to fasting of two age classes, juveniles and sub-adults, during the breeding and non-breeding seasons to determine how these animals utilize lipids and the pattern of fatty acid mobilization from lipid stores during fasting. Four juveniles and 5 sub-adults were fasted for one and two weeks, respectively, and blood samples were collected approximately every …

Responses Of The Florida Mouse (Podomys Floridanus) To Habitat Management, Jason Depue

Electronic Theses and Dissertations

The Florida mouse (Podomys floridanus), a species restricted to the Lake Wales Ridge and the Atlantic Coastal Ridge, is recognized by the Florida Fish and Wildlife Conservation Commission as a species of special concern, highlighting its status as a species that is threatened by habitat loss statewide. Publicly owned lands offer protection for the species, but management is generally focused on protecting biodiversity in general and not a particular species. The response of the Florida mouse to land management practices such as mechanical treatment and prescribed fires is poorly documented. This research examined the population responses of Florida mice on …

Appendix C. Physical Measurements And Chemical Analyses Of China Lake Water Quality, Problems In Environmental Science Course (Biology 493), Colby College, Colby Environmental Assessment Team, Colby College

Colby College Watershed Study: China Lake (2005, 1989)

No abstract provided.

A Watershed Analysis Of China Lake [Presentation], Problems In Environmental Science Course (Biology 493), Colby College, Colby Environmental Assessment Team, Colby College

Colby College Watershed Study: China Lake (2005, 1989)

No abstract provided.

Diversity Of The Unionidae In The Rocky River, Ohio, Robert A. Krebs, Louie J. Rundo

Biological, Geological, and Environmental Faculty Publications

We surveyed the freshwater mussels (Mollusca: Bivalvia: Unionidae) of the Rocky River, Ohio, USA, a river surrounded by suburban development. The survey produced 253 live unionid specimens and 572 empty shells. Most occurred in the west branch. Species living in the West Branch Rocky River included Anodontoides ferussacianus, Elliptio dilatata, Lampsilis cardium, Lampsilis radiata luteola, Lasmigona compressa, Lasmigona costata, Pyganodon grandis grandis, Strophitus undulatus undulatus, and Toxolasma parvus. Two additional species (Utterbackia imbecillis and Villosa iris iris) were represented as dead shells. Three species (Potamilis alatus, Quadrula quadrula and Leptodea fragilis) were found only near the …

Analytical Solutions For Vertical Flow In Unsaturated, Rooted Soils With Variable Surface Fluxes, Fasong Yuan, Zhiming Lu

Biological, Geological, and Environmental Faculty Publications

Analytical solutions to Richards' equation have been derived to describe the distribution of pressure head, water content, and fluid flow for rooted, homogeneous soils with varying surface fluxes. The solutions assume that (i) the constitutive relations for the hydraulic conductivity and water content as function of the pressure head are exponential, (ii) the initial water content distribution is a steady-state distribution, and (iii) the root water uptake is a function of depth. Three simple forms of root water uptake are considered, that is, uniform, stepwise, and exponential functional forms. The lower boundary of the rooted soil profile studied is a …

Density And Cover Preferences Of Black-And-Rufous Elephant-Shrews (Rhynchocyon Petersi) In Chome Forest Reserve, Tanzania, Stephanie Coster, David O. Ribble

Biology Faculty Research

The objective of this study was to determine the density and habitat preference of the Black-andrufous elephant-shrew (Rhynchocyon petersi) in Chome Forest Reserve, Tanzania. Chome Forest (143km²) is located in the South Pare Mountains and provides critical habitat for endangered R. petersi. Twelve 300m transects were cut through the centre of the forest in an east-west direction and the number of elephant-shrew nests within 2.5 meters on each side of the transects was recorded. The mean number of nests per 100m transect (0.39 ± 0.47 [1SE]) translated to a density estimate of 19 elephant-shrews per …

Assessing Cultural And Ecological Variation In Ethnobiological Research: The Importance Of Gender, Jeanine M. Pfeiffer

Faculty Publications, Environmental Studies

Contending that a significant portion of current ethnobiological research continues to overlook cultural variation in traditional ecological knowledge (TEK) and practice, this paper explores the potential impacts of gender-imbalanced research on data collection, hypothesis testing, and the formulation and application of ethnobiological inventories and theories. A multidisciplinary review of over 220 studies addresses commonly held stereotypes underlying gender-imbalanced field research and demonstrates the need for more inclusive, finely-tuned studies which disaggregate indigenous knowledge and practice by gender. The paper outlines factors underlying gender-based spatial and temporal variation in ecosystem exposure and traditional ecological knowledge in rural societies worldwide, and discusses …

The 19th Century Excavation Of Kent's Cavern, England, Donald A. Mcfarlane, Joyce Lundberg

WM Keck Science Faculty Papers

Between 1858 and 1880, William Pengelly developed revolutionary new techniques for the archeological and paleontological excavation of cave deposits. His work at Brixham Cave and Kent’s Cavern, England, yielded tens of thousands of specimens from the mid-Pleistocene to the Holocene, settled the intellectual debate over the co-existence of humans and extinct mammals, and accumulated an unparalleled resource for continued study. Although the Brixham Cave work was thoroughly summarized in print, Pengelly never published the plans of his much more thorough and extensive excavations at Kent’s Cavern. Here we present a reconstructed plan of the Pengelly excavations that we hope will …

The Effects Of Ecotourism On Polar Bear Behavior, Gillian Eckhardt

Electronic Theses and Dissertations

Polar bears spend the majority of their lives on the sea ice, where they gain access to seals and mates. In western Hudson Bay, the sea ice melts for three to four months in the summer, and polar bears there are forced onto land. These bears live on their fat reserves for the duration of the iceless period, until temperatures get colder in the fall and freeze up begins. The aggregation of polar bears near Churchill, Manitoba during the ice free period has led to a thriving tourist industry, with a large influx of tourists visiting Churchill in the fall …

Site Preparation Methods For Restoration Of Non-Native Pasturelands To Native Upland Habitat, Krisann Joie Kosel

Electronic Theses and Dissertations

The flatwoods ecosystem of Florida has been heavily depleted over time but remains one of the most important systems to many threatened and endangered species. Areas that have been converted into non-native pastures may be restored to provide not only this invaluable ecosystem but also restore connectivity of the surrounding ecosystems. The pasture areas on The Disney Wilderness Preserve in central Florida were surveyed, and a conceptual plan for restoration was written in 1996. That same year a pilot study was developed to assess five methods for removing non-native pasture grasses. The treatments studied were single herbicide, single disc, multiple …

Seed And Seedling Ecology Of Acer Saccharum And Acer Platanoides: A Contrast Between Native And Exotic Congeners, Scott J. Meiners

Faculty Research & Creative Activity

The exotic tree, Acer platanoides, is increasing in forests of northeastern North America, largely within the range of its native congener, Acer saccharum. A combination of field and controlled experiments was used on seeds and seedlings of these congeners to determine species characteristics that may be contributing to these floristic changes. Acer platanoides experienced lower rates of seed predation than A. saccharum in field experiments. Differences in the dispersal and allocation characteristics of the two species were small and not likely to explain the relative success of A. platanoides. Greenhouse- grown seedlings of A. platanoides were much larger than those …