Biology Commons^™

Comparison Of Dna Extraction From Cervical Cells Collected In Preservcyt Solution For The Amplification Of Chlamydia Trachomatis, Helen Keegan, Clara Boland, Alison Malkin, Mairead Griffin, Fergus Ryan, Helen Lambkin

Articles

Objective: The aim of this study was to compare and evaluate three methods of DNA extraction for the amplification of Chlamydia trachomatis in uterine cervical samples collected in PreservCyt solution. ThinPrep is the trade name for the slide preparation. Methods: Thirty-eight samples collected in LCx buffer medium, which were identified as C. trachomatis infected by ligase chain reaction (LCR), were selected for this study. DNA from the PreservCyt samples was extracted by three methods: (i) QIAamp kit, (ii) boiling in Tris-EDTA buffer with Chelex purification, and (iii) Proteinase K digestion with Chelex purification. Sample DNA was tested for the presence …

Validation Of A Multiplex Pcr Assay For The Simultaneous Detection Of Human Papillomavirus And Chlamydia Trachomatis In Cervical Preservcyt Samples., Helen Keegan, Alison Malkin, Mairead Griffin, Fergus Ryan, Helen Lambkin

Articles

No abstract provided.

Allosteric Models For Multimeric Proteins : Oxygen-Linked Effector Binding In Hemocyanin., Michael Menze, Nadja Hellman, Heinz Decker, Manfred Grieshaber

Faculty and Staff Scholarship

In many crustaceans, changing concentrations of several low molecular weight compounds modulates hemocyanin oxygen binding, resulting in lower or higher oxygen affinities of the pigment. The nonphysiological effector caffeine and the physiological modulator urate, the latter accumulating in the hemolymph of the lobster Homarus Vulgaris during hypoxia, increase hemocyanin oxygen affinity and decrease cooperativity of oxygen binding. To derive a model that describes the mechanism of allosteric interaction between hemocyanin and oxygen in the presence of urate or caffeine, studies of oxygen, urate, and caffeine binding to hemocyanin were performed. Exposure of lobster hemocyanin to various pH values between 7.25 …

The Challenge Of Environmental Protection, Jennifer Mattei

Biology Faculty Publications

Connecticut is in the midst of a demographic transition to a period of lower population growth. These demographic changes will help check the pressures exerted on the state's natural resources by population growth. Water, air, soil, energy sources, food, fisheries, forests, and biodiversity are common pool resources upon which we depend in ways that transcend political boundaries. Those governing Connecticut should help turn the state into a model of how to manage natural resources by halting forest fragmentation, reducing pollution, and promoting environmental science education.

An Experimental Study Of Substrate Selection In American Toad (Bufo Americanus) Metamorphs, Geoffrey R. Smith

Faculty Publications

No abstract provided.

Survivorship Of Xenosaurus Newmanorum (Sauria: Xenosauridae) From A Seasonal Tropical Environment In Mexico, J. A. Lemos-Espinal, Geoffrey R. Smith, R. E. Ballinger

Faculty Publications

No abstract provided.

Characterization Of Migrating Cells From Ventricular Zone (Vz) To Distant Limbic Structures After Multiple Neonatal Seizures, Melissa Corcia

Seton Hall University Dissertations and Theses (ETDs)

.

Evaluation Of Human Papillomavirus And Chlymdia Tachomatis Infections In The Irish Female Population: Determination Of Prevalence In Adults U Ndergoing Opportunistic Cervical Screening, Helen Keegan

Doctoral

Human papillomaviruses and Chlamydia trachomatis are two of the most common sexually transmitted infections worldwide. They can be detected using urine, swab and cervical scrapings from the anogenital region. Liquid-based cervical cytology is fast becoming the method of choice for the preparation of cervical smears, with the major advantage of extra cellular material from which nucleic acids can be extracted and molecular tests performed. In this project, nucleic acid based methods for the detection of HPV and C. trachomatis and the quantitation and typing of HPV from PreservCyt cervical material were established and applied in an epidemiological study of the …

A Fossil Reef From The Last Interglacial, Western Australia, Benjamin J. Greenstein, John M. Pandolfi, David R. Blakeway

Arts & Sciences Faculty Publications

We report an initial investigation of a Devils Point Event (DPE) style surface exposed approximately 1.5 m above present sea level in a spectacularly preserved reef sequence in southern Exmouth Gulf, Western Australia.

Omnivory By The Small Cosmopolitan Hydromedusa Aglaura Hemistoma, Sean Colin, John H. Costello, William M. Graham, John Higgins Iii

Arts & Sciences Faculty Publications

We investigated the feeding of the small hydromedusa, Aglaura hemistoma (bell diameter < 4 mm), to determine if it occupies a trophic position similar to that of large medusae. Feeding was examined using gut-content analysis of preserved and unpreserved medusae and by analyzing prey-capture events using microvideographic techniques. Analysis of gut contents and prey-capture events revealed that A. hemistoma fed heavily on protistan prey and that it possessed a prey-capture mechanism, specifically a feeding current,that is effective at entraining and capturing protists with low motility. We suggest that many species of small hydromedusae possess prey-capture mechanisms adapted to capture small protistan prey and that many of these small hydromedusae feed omnivorously on microplanktonic prey. The trophic roles of small hydromedusae in different systems are not understood and more studies are needed. However, based on their often high abundances and the cosmopolitan nature, if small hydromedusae …

Flow Patterns Generated By Oblate Medusan Jellyfish: Field Measurements And Laboratory Analyses, John O. Dabiri, Sean Colin, John H. Costello, Morteza Gharib

Arts & Sciences Faculty Publications

Flow patterns generated by medusan swimmers such as jellyfish are known to differ according the morphology of the various animal species. Oblate medusae have been previously observed to generate vortex ring structures during the propulsive cycle. Owing to the inherent physical coupling between locomotor and feeding structures in these animals, the dynamics of vortex ring formation must be robustly tuned to facilitate effective functioning of both systems. To understand how this is achieved, we employed dye visualization techniques on scyphomedusae (Aurelia aurita) observed swimming in their natural marine habitat. The flow created during each propulsive cycle consists of …

Prorocentrum Minimum(Clone Exuv) Is Nutritionally Insufficient, But Not Toxic To The Copepod Acartia Tonsa, Hans G. Dam, Sean Colin

Arts & Sciences Faculty Publications

This study tested whether the dinoflagellate Prorocentrum minimum is nutritionally insufficient or toxic to the copepod Acartia tonsa. Experiments were carried out with adult female A. tonsa and the P. minimum clone Exuv, both isolated from Long Island Sound. Initially, the functional and numerical responses of A. tonsa feeding on exponentially growing P. minimum cells were characterized. These experiments revealed that A. tonsa readily ingested P. minimum cells, up to the equivalent of 200% of body carbon day⁻¹, but egg production was relatively low, with a maximum egg production rate of 22% of body carbon day …

Spontaneous Alternating Behavior In Paramecium Caudatum And Paramecium Multimicronucleatum, Nyron K. A. Bovell

College of Graduate Studies: Theses & Dissertations (1964–2006)

Spontaneous alternating behavior (SAB) describes the tendency of an organism to spontaneously select the unfamiliar direction in a two choice situation. Paramecium is the only microscopic genus in which SAB has been studied. The two earlier studies regarding SAB in Paramecium have come to conclusive, but diametrically opposed results. Designing a single new experiment that incorporates the critical differences in the designs of both studies may help to clarify the results from these earlier studies, and provide an excellent opportunity to better understand the factors that influence SAB.

The overall objective of this research project was to determine whether or …

Genetic Engineering Of Eukaryotic Algae With Special Reference To Chlamydomonas, Mostafa M. El-Sheekh

Turkish Journal of Biology

No abstract provided.

Interactive Anatomy Online, Jennifer Leopold, Thomas Hoeft, Anne M. Maglia

Computer Science Faculty Research & Creative Works

The scientific community and general public can best take advantage of the benefits of 3D digital reconstructions if they are stored in a Web-accessible, easy-to-access database. We describe MorphologyNet/sup /spl copy// a Web-based digital library of realistic, 3D interactive and customizable images of anatomy currently in development at the University of Missouri-Rolla.

Conservation Genetics Of Blue-Spotted Salamanders (Ambystoma Laterale) In The Upper Midwest, Jonathan Michael Eastman

Dissertations and Theses @ UNI

Arguably, the primary intention of conservation genetics is to preserve allelic variability within and among populations, because a lack of genetic diversity may adversely effect the viability of a population subjected to variable environmental conditions. The influence of such evolutionary processes as genetic erosion and inbreeding depression may be pronounced for increasingly small and isolated populations. Blue-spotted salamanders (Ambystoma laterale), though widespread and relatively common throughout northeastern North America, are marked by a pair of peripherally isolated populations at the southwestern boundary of the range. These populations are putative relicts of post-glacial isolation and thus provide a model from which …

Effects Of Nitric Oxide Inhibitors And Scavengers On Gravitropism In Arabidopsis Thaliana, Kristin Russell, Jennifer Jacobi, Jacob Elmore

Opportunities for Undergraduate Research Experience Program (OURE)

No abstract provided.

Cultivation Of Mesophilic Soil Crenarchaeotes In Enrichment Cultures From Plant Roots, Holly M. Simon, Courtney E. Jahn, Luke T. Bergerud, Marek K. Sliwinski, Paul J. Weimer, David K. Willis, Robert M. Goodman

Faculty Work

Because archaea are generally associated with extreme environments, detection of nonthermophilic members belonging to the archaeal division Crenarchaeota over the last decade was unexpected; they are surprisingly ubiquitous and abundant in nonextreme marine and terrestrial habitats. Metabolic characterization of these nonthermophilic crenarchaeotes has been impeded by their intractability toward isolation and growth in culture. From studies employing a combination of cultivation and molecular phylogenetic techniques (PCR– single-strand conformation polymorphism, sequence analysis of 16S rRNA genes, fluorescence in situ hybridization, and real-time PCR), we present evidence here that one of the two dominant phylotypes of Crenarchaeota that colonizes the roots of …

Molecules, Morphology, And Biogeography: An Analysis Of The Phylogeny And Taxonomy Of The Physaria Reediana Species Complex (Brassicaceae), Benjamin R. Grady

Dissertations and Theses @ UNI

The taxonomy of Physaria has continually been in a state of flux, especially certain groups of species within the genus. The Physaria reediana species complex (sensu Rollins) is an example of one of these problematic groups. The systematics of the Physaria reediana species complex was examined by a combination of techniques. A Bayesian analysis of 71 aligned sequences of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA (nrDNA) provided a fairly well-supported phylogeny (mean posterior probability per node: 0.5429) of a portion of the genus, which included the. species in the complex. Recent nodes showed the highest statistical …

The Bipartite Structure Of The Trna M1A58 Methyltransferase From S. Cerevisiae Is Conserved In Humans, Sarah G. Ozanick, Annette M. Krecic, Joshua Andersland, James T. Anderson

Biological Sciences Faculty Research and Publications

Among all types of RNA, tRNA is unique given that it possesses the largest assortment and abundance of modified nucleosides. The methylation at N₁ of adenosine 58 is a conserved modification, occurring in bacterial, archaeal, and eukaryotic tRNAs. In the yeast Saccharomyces cerevisiae, the tRNA 1-methyladenosine 58 (m¹A58) methyltransferase (Mtase) is a two-subunit enzyme encoded by the essential genes TRM6 (GCD10) and TRM61 (GCD14). While the significance of many tRNA modifications is poorly understood, methylation of A58 is known to be critical for maintaining the stability of initiator tRNA^Met in yeast. …

Seed Storage Reserves And Glucosinolates In Brassica Rapa L. Grown On The International Space Station, M. E. Musgrave, Anxiu Kuang, L. K. Tuominen, L. H. Levine, R. C. Morrow

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Although plants are envisioned to play a central role in life support systems for future long-duration space travel, plant growth in space has been problematic due to horticultural problems of nutrient delivery and gas resupply posed by the weightless environment. Iterative improvement in hardware designed for growth of plants on orbital platforms now provides confidence that plants can perform well in microgravity, enabling investigation of their nutritional characteristics. Plants of B. rapa (cv. Astroplants) were grown in the Biomass Production System on the International Space Station. Flowers were hand-pollinated and seeds were produced prior to harvest at 39 days after …

An Efficient Method For Supporting Multiple Types Of Services On Smart Server, Chang-Soo Kim, Yu-Hyeon Bak, Seung-Joe Bae, Jin-Mee Kim, Sang-Min Woo, Seung-Hyup Jeon, Won-Jae Lee, Hag-Young Kim

Electrical and Computer Engineering Faculty Research & Creative Works

No abstract provided.

A New Microsensor System For Plant Root Zone Monitoring, Chang-Soo Kim, Sandeep Sathyan, D. M. Porterfield

Electrical and Computer Engineering Faculty Research & Creative Works

The objective of this work is to develop a new microsensor system that can monitor dissolved oxygen and hydration environment at the plant root zone. A miniaturized plant growth system is prepared including the root zone layer, either a porous ceramic tube or porous ceramic wafer on which the plant is grown, and an underlying fluidic channel to deliver nutrients and water to the root zone. We demonstrate the feasibility of using a flexible microsensor array for dissolved oxygen detection, and a four-electrode impedance microelectrode for wetness detection on the surface of a porous tube nutrient delivery system. The unique …

Reproductive Effort Is Inversely Proportional To Average Adult Life Span., Eric Charnov

Biology Faculty & Staff Publications

Background: Forty years ago, G.C. Williams predicted that reproductive effort should be inversely related to the average adult life span across species. Aim: Use allometric life-history theory to refine that prediction. Result: Reproductive effort should be inversely proportional to average adult life span, a −1 scaling rule.

Mammal Life-History Evolution With Size-Dependent Mortality, Eric Charnov

Biology Faculty & Staff Publications

Size dependence of mortality over the ontogeny is added to life-history optimization schemes for mammals