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Articles 91 - 120 of 330
Full-Text Articles in Biology
Molecular Evolution Of Odorant-Binding Protein Genes Os-E And Os-F In Drosophila, Daria S. Hekmat-Scafe, Robert L. Dorit, John R. Carlson
Molecular Evolution Of Odorant-Binding Protein Genes Os-E And Os-F In Drosophila, Daria S. Hekmat-Scafe, Robert L. Dorit, John R. Carlson
Biological Sciences: Faculty Publications
The Drosophila olfactory genes OS-E and OS-F are members of a family of genes that encode insect odorant-binding proteins (OBPs). OBPs are believed to transport hydrophobic odorants through the aqueous fluid within olfactory sensilla to the underlying receptor proteins. The recent discovery of a large family of olfactory receptor genes in Drosophila raises new questions about the function, diversity, regulation, and evolution of the OBP family. We have investigated the OS-E and OS-F genes in a variety of Drosophila species. These studies highlight potential regions of functional significance in the OS-E and OS-F proteins, which may include a region required …
An Ulstrastructural Study Of Acetabularia Acetabulum, Rachel Vangilder
An Ulstrastructural Study Of Acetabularia Acetabulum, Rachel Vangilder
Master's Theses
Calcification within the cell and the cell wall Acetabularia acetabulum, a large single cell alga, is examined through an ultrastructural investigation, electron diffraction, and an elemental analysis. These methods suggest that amorphous calcium carbonate is located adjacent to and within the cell wall. The location of the crystals within and directly outside the cell wall suggests the calcium is obtained from the surrounding environment. Fibrillar structures are seen through SEM in areas where calcification is occurring. This fibrillar material appears to serve as the organic matrix where calcification initiates. Intracellularly electron dense materials is seen in the chloroplasts. This …
Invertebrate Carcasses As A Resource For Competing Aedes Albopictus And Aedes Aegypti (Diptera : Culicidae), M P. Daugherty, B W. Alto, Steven A. Juliano
Invertebrate Carcasses As A Resource For Competing Aedes Albopictus And Aedes Aegypti (Diptera : Culicidae), M P. Daugherty, B W. Alto, Steven A. Juliano
Faculty Publications – Biological Sciences
However, little is known of the role of these carcasses in other containers, which also receive leaf fall and stemflow inputs. This experiment investigated effects of accumulated invertebrate carcasses as a resource for two competing mosquitoes, Aedes albopictus (Skuse) and Aedes aegypti (L.) whether either species differentially benefited from accumulated carcasses, and if such a benefit affected interspecific competition. First, we measured accumulation of invertebrate carcasses in standard containers at a field site. We then used a replacement series with five different species ratios at the same total density: and varied the input of invertebrate carcasses [dead Drosophila melanogaster (Meigen) …
On The Nature Of Norms: Biology, Morality, And The Disruption Of Order, Owen D. Jones
On The Nature Of Norms: Biology, Morality, And The Disruption Of Order, Owen D. Jones
Vanderbilt Law School Faculty Publications
This essay discusses the legal implications of bio-behavioral underpinnings to norms, morality, and economic order. It first discusses the recent book "The Great Disruption: Human Nature and the Reconstitution of Social Order," in which Francis Fukuyama explores the importance of evolved human nature to the reconstruction of social order and a thriving economy. It then addresses the extent to which we can usefully view law-relevant norms as products of evolutionary - as well as economic - processes.
Identification Of Fungal Pathogens Of Grasshoppers, M. J. Bidochka, Donald W. Roberts
Identification Of Fungal Pathogens Of Grasshoppers, M. J. Bidochka, Donald W. Roberts
Biology Faculty Publications
No abstract provided.
Nesting Of Rose-Throated Becard Pachyramphus Aglaiae (Passeriformes: Incertae Sedis) And Clay-Colored Robin Turdus Grayi (Passeriformes: Turdidae) In Hidalgo County, Texas, Timothy Brush
School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)
No abstract provided.
Mutagenesis And Cloning Of Photosynesis Genes Of The Filamentous Bacterium, Chloroflexus Aurantiacus, Kristi L. Berger '00
Mutagenesis And Cloning Of Photosynesis Genes Of The Filamentous Bacterium, Chloroflexus Aurantiacus, Kristi L. Berger '00
Honors Projects
Chloroflexus aurantiacus is a green non-sulfur bacterium that preferentially grows photosynthetically under lighted, anaerobic conditions. The biosynthetic pathway for one of the photosynthetic pigments, bacteriochlorophyll c, is not well understood but may share common steps with the better understood bacteriochlorophyll a pathway. Cultures of C. aurantiacus were mutagenized in order to produce photosynthetic pigment mutants to be used later in the determination of intermediate compounds in the pathway for bacteriochlorophyll c synthesis. However, mutants with photosynthetic pigment deficiencies were not produced due to experimental difficulties. The photosynthesis genes of C. aurantiacus, such as the bchXYZ genes, are of evolutionary interest …
An Assay For The Estimation Of Organic Content In Unknown Biological Samples, William J. Schneider '00
An Assay For The Estimation Of Organic Content In Unknown Biological Samples, William J. Schneider '00
Honors Projects
Potassium dichromate, in an acidic solution, will oxidize organic material. The reduction to the chromium ion is associated with a color change, which can be measured as a change in absorbance using a spectrophotometer. The degree of change is linearly related to the total energy contained in a sample and this information can be used to predict the energy content of unknown samples. However, the slopes of these relationships are significantly different for carbohydrates and proteins. It is hypothesized that these variations are due to differences in the reaction kinetics and that these differences can be used to predict the …
Test Of Intron Predictions Reveals Novel Splice Sites, Alternatively Spliced Mrnas And New Introns In Meiotically Regulated Genes Of Yeast, Carrie Davis, Leslie Grate, Marc Spingola, Manuel Ares
Test Of Intron Predictions Reveals Novel Splice Sites, Alternatively Spliced Mrnas And New Introns In Meiotically Regulated Genes Of Yeast, Carrie Davis, Leslie Grate, Marc Spingola, Manuel Ares
Biology Department Faculty Works
Correct identification of all introns is necessary to discern the protein-coding potential of a eukaryotic genome. The existence of most of the spliceosomal introns predicted in the genome of Saccharomyces cerevisiae remains unsupported by molecular evidence. We tested the intron predictions for 87 introns predicted to be present in non-ribosomal protein genes, more than a third of all known or suspected introns in the yeast genome. Evidence supporting 61 of these predictions was obtained, 20 predicted intron sequences were not spliced and six predictions identified an intron-containing region but failed to specify the correct splice sites, yielding a successful prediction …
Conjugal Transfer Of A Transcriptional Fusion Plasmid Containing The Toluene 2-Monooxygenase Gene Promoter From An E.Coli Host To A Parental Strain, Burkholderia Cepacia G4, Ross Martin Vander Noot
Conjugal Transfer Of A Transcriptional Fusion Plasmid Containing The Toluene 2-Monooxygenase Gene Promoter From An E.Coli Host To A Parental Strain, Burkholderia Cepacia G4, Ross Martin Vander Noot
Honors Capstone Projects and Theses
No abstract provided.
The Age Of The Woolly Rhino From Dream Cave, Derbyshire, Uk, Donald A. Mcfarlane, Joyce Lundberg, Derek C. Ford
The Age Of The Woolly Rhino From Dream Cave, Derbyshire, Uk, Donald A. Mcfarlane, Joyce Lundberg, Derek C. Ford
WM Keck Science Faculty Papers
The Dream Cave woolly rhinoceros, Coelodonta antiquitatis, is a "classic" specimen of a "cold-stage" fossil fauna from central England. The find was illustrated and described by Dean William Buckland in his seminal tome Reliquiae Diluvianae (1823) during the first half of the 19th century, and made a significant contribution to the development of Buckland's views on the origin of extinct and extirpated fossil vertebrates. The report presents the first, albeit indirect, radiometric dates on the specimen, and argues that the animal fell into the cave just before 37,000 years BP, during the middle of Marine Isotope Stage 3 Interstadial (41 …
Variation And Heritability Of Phenology In The Fungus Monilinia Vaccinii-Corymbosi On Blueberry, Jeffery S. Lehman, Peter V. Oudemans
Variation And Heritability Of Phenology In The Fungus Monilinia Vaccinii-Corymbosi On Blueberry, Jeffery S. Lehman, Peter V. Oudemans
Biology and Earth Science Faculty Scholarship
The germination of field-collected pseudosclerotia and the development of apothecia from eight New Jersey populations of the mummy berry fungus Monilinia vaccinii-corymbosi were evaluated under controlled conditions in the greenhouse. Development data for apothecia were used to describe the timing of apothecium formation and to estimate broad- and narrow-sense heritabilities of fungal phenology. Mean development times for the formation of apothecia ranged from 35.4 to 54.7 days. The mean development times for populations collected from early-season cv. Weymouth ranged from 35.4 to 39.6 days and were significantly shorter than the development times for three of the four populations collected from …
Isolating Pcr-Quality Dna From Human Feces With A Soil Dna Kit, Brian G. Clement, Christopher L. Kitts
Isolating Pcr-Quality Dna From Human Feces With A Soil Dna Kit, Brian G. Clement, Christopher L. Kitts
Biological Sciences
No abstract provided.
Disruption Of The Borrelia Burgdorferi Gac Gene, Encoding The Naturally Synthesized Gyra C-Terminal Domain, Scott W. Knight, Betsy J. Kimmel, Christian H. Eggers, D. Scott Samuels
Disruption Of The Borrelia Burgdorferi Gac Gene, Encoding The Naturally Synthesized Gyra C-Terminal Domain, Scott W. Knight, Betsy J. Kimmel, Christian H. Eggers, D. Scott Samuels
Biological Sciences Faculty Publications
The C-terminal domain of the A subunit of DNA gyrase, which we term Gac, is naturally synthesized in Borrelia burgdorferi as an abundant DNA-binding protein. Full-length GyrA, which includes the C-terminal domain, is also synthesized by the spirochete and functions as a subunit of DNA gyrase, We have disrupted synthesis of Gac as an independent protein and demonstrated that it is not essential for growth in a coumarin-resistant background. We detected no alterations in DNA maintenance, condensation, or topology In B. burgdorferi lacking this small DNA-binding protein.
Abnormal Pigmentation In A Franklin's Gull, Philip C. Chu
Abnormal Pigmentation In A Franklin's Gull, Philip C. Chu
Biology Faculty Publications
No abstract provided.
Molecular And Biological Characterization Of A Tanapox Virus Tnf-Binding Protein And The Complete Nucleotide Sequence Of The Psti-J Genomic Fragment, Mini Paulose-Murphy
Molecular And Biological Characterization Of A Tanapox Virus Tnf-Binding Protein And The Complete Nucleotide Sequence Of The Psti-J Genomic Fragment, Mini Paulose-Murphy
Dissertations
Most of the poxvirus encoded virulence factors have been identified as proteins that are secreted from infected host cells. Some of these secretory proteins impede host immune defenses. We have previously demonstrated that tanapox virus (TPV) infected cells secrete a 38 kDa glycopeptide that binds to human (h) interferon-γ, h-interleukin (IL)-2, and hIL-5. We now show an additional activity in the supernatant from TPV infected cells that down-regulates the expression of tumor necrosis factor-a (TNF-α) induced cell adhesion molecule gene expression. This activity was not detected in mock infected cells. Enzyme linked immunosorbent assays (ELISA) on primary human endothelial cells, …
Identification And Classification Of Coryneform Bacteria Isolated From Bovine Mammary Glands, Jeffrey L. Watts
Identification And Classification Of Coryneform Bacteria Isolated From Bovine Mammary Glands, Jeffrey L. Watts
Dissertations
Coryneform bacteria are frequently isolated from bovine mastitis and these infections are associated with economic losses. Corynehacterium bovis, a lipid requiring species, has been the most frequently isolated coryneform from the milk of infected bovine mammary glands. However, the taxonomic status of this organism is uncertain. In the current study, a polyphasic approach was used to identify coryneform bacteria isolated from bovine mastitis and determ ine the phylogenetic relationships among the identified species. A total of 212 coryneform bacteria isolated from bovine mastitis was obtained from mastitis reference laboratories in the United States and Canada. Presumptive identification based upon …
Target-And Stage-Dependent Influences On Axonal Outgrowth Of An Identified Motorneuron In Leech Cns Explants, April Crowley
Target-And Stage-Dependent Influences On Axonal Outgrowth Of An Identified Motorneuron In Leech Cns Explants, April Crowley
Masters Theses
The neural circuit that controls rhythmic contraction of the hearts in the medicinal leech, Hirudo medicinalis, provides an amenable model to investigate pathfinding and target selection. The bilaterally paired heart tubes (HT) in leech are eventually innervated by the heart excitor motomeuron (HE) following a period of remodeling that requires the presence of the HT. To begin characterizing possible HT-derived cues, co-cultures of CNS with innervated HT have been used. Previous work demonstrated that young, but not adult, HT was able to support profuse general outgrowth, but the HE extended few projections onto HT of any age. Current studies …
Molecular Taxonomy Of The Lichen Genus Heterodermia, Linda Hirschberger
Molecular Taxonomy Of The Lichen Genus Heterodermia, Linda Hirschberger
Seton Hall University Dissertations and Theses (ETDs)
Heterodermia is lichen consisting of a fungus and algae. Morphology and chemistry cannot provide definitive evidence for the identity of Heterodermia because various species have very similar morphologies that cannot always be distinguished. Other species only differ by the presence or absence of secondary chemicals detected. by specific chemical tests, which makes distinguishing between two species difficult. The objective of this project is to use Restriction Fragment Length Polymorphisms (RFLPs) to determine how closely related each Heterodermia species is to one another. RFLPs will also be used to determine how closely related a species is to its equal in another …
The Hp1 Protein Family: Getting A Grip On Chromatin, J Eissenberg, Sarah C.R. Elgin
The Hp1 Protein Family: Getting A Grip On Chromatin, J Eissenberg, Sarah C.R. Elgin
Biology Faculty Research
HP1 was first described in Drosophila as a heterochromatin-associated protein with dosage-dependent effects on heterochromatin-induced gene silencing. Recently, membership of the HP1 protein family has expanded tremendously. A number of intriguing interactions between HP1 and other proteins have been described, implicating HP1 in gene regulation, DNA replication, and nuclear architecture.
Effects Of The Zebra Mussel On Nitrogen Dynamics And The Microbial Community At The Sediment-Water Interface, Peter J. Lavrentyev, Wayne S. Gardner, Longyuan Yang
Effects Of The Zebra Mussel On Nitrogen Dynamics And The Microbial Community At The Sediment-Water Interface, Peter J. Lavrentyev, Wayne S. Gardner, Longyuan Yang
Biology Faculty Research
A flow-through experiment was conducted on intact cores of sediments from Saginaw Bay, Lake Huron, to examine how trophic interactions between filter-feeding bivalve mussels and microbial populations could affect nitrogen dynamics at the sediment-water interface. The zebra mussels used in this experiment removed a large proportion of protozoa and phytoplankton from the overlying water, particularly heterotrophic nanoplankton (up to 82%), while bacterial populations showed less change. A 3-fold decrease in the protozoan to bacterial carbon ratio corresponded to a 2.5-fold increase in relative ammonium removal rates as estimated from the dark loss of N-15-ammonium. Excretion by the bivalves also increased …
The Docking Stage Of Yeast Vacuole Fusion Requires The Transfer Of Proteins From A Cis-Snare Complex To A Rab/Ypt Protein, Albert Price, Darren Seals, William Wickner, Christian Ungermann
The Docking Stage Of Yeast Vacuole Fusion Requires The Transfer Of Proteins From A Cis-Snare Complex To A Rab/Ypt Protein, Albert Price, Darren Seals, William Wickner, Christian Ungermann
Dartmouth Scholarship
The homotypic fusion of yeast vacuoles requires Sec18p (NSF)-driven priming to allow vacuole docking, but the mechanism that links priming and docking is unknown. We find that a large multisubunit protein called the Vam2/6p complex is bound to cis-paired SNAP receptors (SNAREs) on isolated vacuoles. This association of the Vam2/6p complex with the cis-SNARE complex is disrupted during priming. The Vam2/6p complex then binds to Ypt7p, a guanosine triphosphate binding protein of the Rab family, to initiate productive contact between vacuoles. Thus, cis-SNARE complexes can contain Rab/Ypt effectors, and these effectors can be mobilized by NSF/Sec18p-driven priming, allowing their direct …
Identification Of Potential Vaccine And Drug Target Candidates By Expressed Sequence Tag Analysis And Immunoscreening Of Onchocerca Volvulus Larval Cdna Libraries, Michelle Lizotte-Waniewski, Wilson Tawe, David B. Guiliano, Wenhong Lu, Jing Liu, Steven A. Williams, Sara Lustigman
Identification Of Potential Vaccine And Drug Target Candidates By Expressed Sequence Tag Analysis And Immunoscreening Of Onchocerca Volvulus Larval Cdna Libraries, Michelle Lizotte-Waniewski, Wilson Tawe, David B. Guiliano, Wenhong Lu, Jing Liu, Steven A. Williams, Sara Lustigman
Biological Sciences: Faculty Publications
The search for appropriate vaccine candidates and drug targets against onchocerciasis has so far been confronted with several limitations due to the unavailability of biological material, appropriate molecular resources, and knowledge of the parasite biology. To identify targets for vaccine or chemotherapy development we have undertaken two approaches. First, cDNA expression libraries were constructed from life cycle stages that are critical for establishment of Onchocerca volvulus infection, the third-stage larvae (L3) and the molting L3. A gene discovery effort was then initiated by random expressed sequence tag analysis of 5,506 cDNA clones. Cluster analyses showed that many of the transcripts …
A Developmentally Regulated Deletion Element With Long Terminal Repeats Has Cis-Acting Sequences In The Flanking Dna, Namrata S. Patil, Kathleen M. Karrer
A Developmentally Regulated Deletion Element With Long Terminal Repeats Has Cis-Acting Sequences In The Flanking Dna, Namrata S. Patil, Kathleen M. Karrer
Biological Sciences Faculty Research and Publications
Approximately 6000 specific DNA deletion events occur during development of the somatic macronucleus of the ciliate Tetrahymena. The eliminated Tlr1 element is 13 kb or more in length and has an 825 bp inverted repeat near the rearrangement junctions. A functional analysis of the cis-acting sequences required for Tlr1 rearrangement was performed. A construct consisting of the entire inverted repeat and several hundred base pairs of flanking DNA on each side was rearranged accurately in vivo and displayed junctional variability similar to the chromosomal Tlr1 rearrangement. Thus, 11 kb or more of internal element DNA is not required …
Type I Nitroreductases In Soil Enterobacteria Reduce Tnt (2,4,6-Trinitrotoluene) And Rdx (Hexahydro-1,3,5-Trinitro-1,3,5-Triazine), Christopher Kitts, Chad E. Green, Rebecca A. Otley, Marc A. Alvarez, Pat J. Unkefer
Type I Nitroreductases In Soil Enterobacteria Reduce Tnt (2,4,6-Trinitrotoluene) And Rdx (Hexahydro-1,3,5-Trinitro-1,3,5-Triazine), Christopher Kitts, Chad E. Green, Rebecca A. Otley, Marc A. Alvarez, Pat J. Unkefer
Biological Sciences
Many enteric bacteria express a type I oxygen-insensitive nitroreductase, which reduces nitro groups on many different nitroaromatic compounds under aerobic conditions. Enzymatic reduction of nitramines was also documented in enteric bacteria under anaerobic conditions. This study indicates that nitramine reduction in enteric bacteria is carried out by the type I, or oxygen-insensitive nitroreductase, rather than a type II enzyme. The enteric bacterium Morganella morganii strain B2 with documented hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) nitroreductase activity, and Enterobacter cloacae strain 96-3 with documented 2,4,6-trinitrotoluene (TNT) nitroreductase activity, were used here to show that the explosives TNT and RDX were both reduced by a type …
Optical Discrimination Of A Phytoplankton Species In Natural Mixed Populations, David F. Millie, Mark A. Moline, Oscar Schofield
Optical Discrimination Of A Phytoplankton Species In Natural Mixed Populations, David F. Millie, Mark A. Moline, Oscar Schofield
Biological Sciences
Developing optical detection techniques for discriminating particular phytoplankon species in mixed assemblages has long been a goal of aquatic scientists. Previously, a processing algorithm for phytoplankton absorption spectra was reported that suggested detection of the red tide dinoflagellate Gymnodinium breve was possible. The algorithm evaluated the fourth derivative of the particulate absorption spectrum of an unknown sample and compared it to a standard fourth derivative spectrum for G. breve using a similarity index. We report here the first-time application of this technique to the detection of G. breve in natural, mixed phytoplankton communities. Pigment and spectral absorption data were collected …
A Bacteriophage-Like Particle From Bartonella Bacilliformis, Kent D. Barbian, Michael F. Minnick
A Bacteriophage-Like Particle From Bartonella Bacilliformis, Kent D. Barbian, Michael F. Minnick
Biological Sciences Faculty Publications
Bartonella bacilliformis and Bartonella henselae, the respective agents of Oroya fever and cat-scratch disease in humans, are known to produce bacteriophage-like particles (BLPs) that package 14 kbp segments of the host chromosome. Data from this study suggest that other Bartonella species including Bartonella quintana, Bartonella doshiae and Bartonella grahamii also contain similar BLPs, as evidenced by the presence of a 14 kbp extrachromosomal DNA element in their genomes, whereas Bartonella elizabethae and Bartonella clarridgeiae do not. A purification scheme utilizing chloroform, DNase I and centrifugation was devised to isolate BLPs from B. bacilliformis. Intact BLPs were observed by transmission electron …
Periphyton Production On Wood And Sediment: Substratum-Specific Response To Laboratory And Whole-Lake Nutrient Manipulations, Yvonne Vadeboncoeur, David M. Lodge
Periphyton Production On Wood And Sediment: Substratum-Specific Response To Laboratory And Whole-Lake Nutrient Manipulations, Yvonne Vadeboncoeur, David M. Lodge
Biological Sciences Faculty Publications
Substratum heterogeneity is a large source of variability in periphyton production, but the influence of substratum on periphyton response to experimental manipulations is rarely measured. Using laboratory and whole-lake experiments, we compared area-specific primary production of periphyton on wood (epixylon) and sediment (epipelon), and tested whether periphyton on the 2 substrata responded differently to water-column fertilization. In the laboratory, natural periphyton assemblages on wood or sediment were exposed to 1 of 6 treatments in a fully factorial (light [250, 70, or 10 μmol m−2 s−1] × nutrient [control or + N and P]) experiment. We measured 14 …
A Summer Survey Of The Birds At Two Eastern Nebraska Wetlands, Kristine T. Phipps
A Summer Survey Of The Birds At Two Eastern Nebraska Wetlands, Kristine T. Phipps
Biology Faculty Publications
This study compares the avian species diversity at two eastern Nebraska wetlands that differ in their relative isolation from an urban environment. Birds were surveyed by the point Count method twice weekly at each site during June of 1998. Diversity was measured using species richness and species evenness. The percentage of bird species observed that depend on wetlands for breeding was also compared. Results suggest that both species richness and evenness, as determined by the Shannon-Wiener index, were higher at the wetland located in a network of other marsh areas and agricultural land than at the suburban wetland. Immigration of …
The Advantages Of Clonal Integration Under Different Ecological Conditions: A Community-Wide Test, Steven C. Pennings, Ragan M. Callaway
The Advantages Of Clonal Integration Under Different Ecological Conditions: A Community-Wide Test, Steven C. Pennings, Ragan M. Callaway
Biological Sciences Faculty Publications
The "connectedness" of clonal plants has been shown to promote survival and growth in a variety of single-species, single-factor studies, but experiments comparing the relative advantages of clonality across multiple habitats and species are rare, raising the concern that generalizations about the benefits of clonality might be biased by the particular species or habitat studied. We studied the importance of clonal integration in southeastern USA salt marsh plants, using all six of the common clonal species in the community, by following the success of intact and severed clonal fragments invading three habitat treatments. Clonal integration was most important for growth …