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2000

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Articles 31 - 60 of 330

Full-Text Articles in Biology

Does A Rigid Body Limit Maneuverability?, Jeffrey A. Walker Phd Oct 2000

Does A Rigid Body Limit Maneuverability?, Jeffrey A. Walker Phd

Faculty Publications

Whether a rigid body limits maneuverability depends on how maneuverability is defined. By the current definition, the minimum radius of the turn, a rigid-bodied, spotted boxfish Ostracion meleagris approaches maximum maneuverability, i.e. it can spin around with minimum turning radii near zero. The radius of the minimum space required to turn is an alternative measure of maneuverability. By this definition, O. meleagris is not very maneuverable. The observed space required by O. meleagris to turn is slightly greater than its theoretical minimum but much greater than that of highly flexible fish. Agility, the rate of turning, is related to maneuverability. …

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The Aquaculture Of Non-Endemic Species In Western Australia Silver Perch (Bidyanus Bidyanus), Tina Thorne, H. G. Brayford Oct 2000

The Aquaculture Of Non-Endemic Species In Western Australia Silver Perch (Bidyanus Bidyanus), Tina Thorne, H. G. Brayford

Fisheries Management Papers

The development of commercial aquaculture of a non-endemic species such as silver perch raises a number of important issues relating to translocation. These include the potential of the introduced species to: impact on genetic diversity; introduce disease; and impact on - the natural environment; and - the biodiversity of native species. This Management Paper has been drafted in liaison with the Department of Environmental Protection in accordance with the principles of rish assessment, taking into account the above issues. It is considered that the implementation of this policy will protect the natural environment and native fish species. It will also …

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Linear And Nonlinear Measures Predict Swimming In The Leech, C. J. Cellucci, Peter Brodfuehrer, H. Acera-Pozzi, E. Dobrovolny, J. Engler, R. Los, R. Thompson, Alfonso M. Albano Oct 2000

Linear And Nonlinear Measures Predict Swimming In The Leech, C. J. Cellucci, Peter Brodfuehrer, H. Acera-Pozzi, E. Dobrovolny, J. Engler, R. Los, R. Thompson, Alfonso M. Albano

Biology Faculty Research and Scholarship

Stimulation of a trigger interneuron of an isolated nerve cord preparation of the medicinal leech, Hirudo medicinalis, sometimes leads to swimming; sometimes it does not. We investigate signals transmitted in the ventral cord of the leech after stimulation and seek quantitative measures that would make it possible to distinguish signals that predict swimming from those that do not. We find that a number of linear as well as nonlinear measures provide statistically significant distinctions between the two kinds of signals. The linear measures are the time dependence of (i) the standard deviation and (ii) the autocorrelation function at a small …

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Facilitation May Buffer Competitive Effects: Indirect And Diffuse Interactions Among Salt Marsh Plants, Ragan M. Callaway, Steven C. Pennings Oct 2000

Facilitation May Buffer Competitive Effects: Indirect And Diffuse Interactions Among Salt Marsh Plants, Ragan M. Callaway, Steven C. Pennings

Biological Sciences Faculty Publications

Direct interactions among plant species may be highly modified by indirect or diffuse effects within a multispecies community. We investigated the direct and diffuse effects of two salt marsh perennials, Monanthechloe littoralis and Arthrocnemum subterminale, on winter annuals and the perennial herb Limonium californicum in a salt marsh in central California. In permanent plots, Monanthechloe had expanded substantially in the upper marsh over the past 13 yr, while Arthrocnemum and all annual species had decreased. These dynamics suggest that Monanthechloe may directly outcompete most other species in the upper marsh. In contrast, Arthrocnemum is known to facilitate some annual species. …

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Varying The Abundance Of O Antigen In Rhizobium Etli And Its Effect On Symbiosis With Phaseolus Vulgaris, K. Dale Noel, Lennart Scott Forsberg, Russell W. Carlson Oct 2000

Varying The Abundance Of O Antigen In Rhizobium Etli And Its Effect On Symbiosis With Phaseolus Vulgaris, K. Dale Noel, Lennart Scott Forsberg, Russell W. Carlson

Biological Sciences Faculty Research and Publications

Judged by migration of its lipopolysaccharide (LPS) in gel electrophoresis, the O antigen of Rhizobium etli mutant strain CE166 was apparently of normal size. However, its LPS sugar composition and staining of the LPS bands after electrophoresis indicated that the proportion of its LPS molecules that possessed O antigen was only 40% of the wild-type value. Its LPS also differed from the wild type by lacking quinovosamine (2-amino-2,6-dideoxyglucose). Both of these defects were due to a single genetic locus carrying a Tn5 insertion. The deficiency in O-antigen amount, but not the absence of quinovosamine, was suppressed by transferring into …

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Is Reproduction By Tree Swallows (Tachycineta Bicolor) Cost-Free?, Michael T. Murphy Oct 2000

Is Reproduction By Tree Swallows (Tachycineta Bicolor) Cost-Free?, Michael T. Murphy

Biology Faculty Publications and Presentations

We manipulated brood sizes of Tree Swallows (Tachycineta bicolor) in 1996 and 1997 to test for the existence of intra- and intergenerational costs of reproduction. Modal clutch size was six eggs, but experimental brood sizes ranged from two to nine young. Nestling starvation was higher in 1996 (and dependent on brood size) than in 1997, but in both years enlargement of brood size resulted in increased productivity. Nestling mass near fledging was negatively correlated with brood size, but tarsus length and wing chord were not. Food deliveries by parents increased steadily between broods of two to six young but then …

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A Conserved Mechanism For Post-Transcriptional Gene Silencing?, Eleanor M. Maine Sep 2000

A Conserved Mechanism For Post-Transcriptional Gene Silencing?, Eleanor M. Maine

Biology - All Scholarship

Proteins with homology to RNA-directed RNA polymerases function in post-transcriptional gene silencing: in quelling in the fungus Neurospora crassa, RNAi in the nematode Caenorhabditis elegans, and co-suppression in the mustard plant Arabidopsis thaliana. These findings are consistent with a conserved mechanism operating in these diverse species.

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Biology Department Publications: 2000, Sam Lindgren Sep 2000

Biology Department Publications: 2000, Sam Lindgren

Biology Department Bibliographies

This document is a noncomprehensive list of publications made by graduate students, post-doctoral researchers, and faculty members in the Biology Department in 2000. It indexes those publications available through major databases, and serves as an archival record of the Department’s robust scholarly contributions.

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An Unusual Pulse In Recruitment Of Two Reef Fishes In The Galápagos Islands Coincident With The 1997–1998 El Niño, Benjamin I. Ruttenberg Sep 2000

An Unusual Pulse In Recruitment Of Two Reef Fishes In The Galápagos Islands Coincident With The 1997–1998 El Niño, Benjamin I. Ruttenberg

Biological Sciences

No abstract provided.

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Regulation Of Sup Expression Identifies Multiple Regulators Involved In Arabidopsis Floral Meristem Development, Hajime Sakai, Beth A. Krizek, Steven E. Jacobsen, Elliot M. Meyerowitz Sep 2000

Regulation Of Sup Expression Identifies Multiple Regulators Involved In Arabidopsis Floral Meristem Development, Hajime Sakai, Beth A. Krizek, Steven E. Jacobsen, Elliot M. Meyerowitz

Faculty Publications

During the course of flower development, floral homeotic genes are expressed in defined concentric regions of floral meristems called whorls. The SUPERMAN ( SUP, also called FLO10 ) gene, which encodes a C2H2-type zinc finger protein, is involved in maintenance of the stamen/carpel whorl boundary (the boundary between whorl 3 and whorl 4) in Arabidopsis. Here, we show that the regulation of SUP expression in floral meristems is complex, consisting of two distinct phases, initiation and maintenance. The floral meristem identity gene LEAFY ( LFY ) plays a role in the initiation phase through at least two pathways, which …

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Have We Forgotten The Forest Because Of The Trees?, Stefan A. Schnitzer, Walter P. Carson Sep 2000

Have We Forgotten The Forest Because Of The Trees?, Stefan A. Schnitzer, Walter P. Carson

Biological Sciences Faculty Research and Publications

No abstract provided.

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Los Anfibios Del Monumento Natural Barro Colorado, Parque Nacional Soberania Y Areas Adyacentes, Rafael O. De Sá Sep 2000

Los Anfibios Del Monumento Natural Barro Colorado, Parque Nacional Soberania Y Areas Adyacentes, Rafael O. De Sá

Biology Faculty Publications

This book focuses on the batrachofauna of Barro Colorado Nature Monument. Soberania National Park and adjacent areas. It complements other recent publications on Neotropical amphibians such as those by Rodriguez and Duellman ( 1994) on Peru, Achaval and Olmos (1995) on Uruguay, and Meyer and Foster (1996) on Belize. The goal of these books is to reach a wider audience than the more technical batrachofaunal summaries-e.g., Frogs of Boracéia (Heyer 1990). Amphibians of Argentina (Cei 1985), and Frogs of the Genus Eleutherodactylus in Western Ecuador (Lynch and Duellman 1997). These geographically focused guides are especially useful to students, amateur biologists, …

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Molecular Cloning And Analysis Of The Modification Genes Of The Kpnbl Restriction- Modification System Of Klebsiella Pneumoniae Strain Gm236, Vernon Robert Chin Sep 2000

Molecular Cloning And Analysis Of The Modification Genes Of The Kpnbl Restriction- Modification System Of Klebsiella Pneumoniae Strain Gm236, Vernon Robert Chin

Loma Linda University Electronic Theses, Dissertations & Projects

The KpnBl restriction-modification (R-M) system has been recognized for some time in Klebsiella pneumoniae strain GM236. Previously the restriction subunit (hsdRkpmBI) was cloned and sequenced, based on the sequence infonnation the KpnBl RM system was suspected to be either a type I or type III R-M system In this project the modification genes of KpnBl were cloned into a pMECA plasmid by using a modification expression method. The modification genes were identified on an 8.2 kb EcoRl fragment from a chromosomal library of the GM236 strain. The complete 8.2 kb fragment was sequenced and …

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Tid1/Rdh54 Promotes Colocalization Of Rad51 And Dmc1 During Meiotic Recombination, Stephen L. Gasior Sep 2000

Tid1/Rdh54 Promotes Colocalization Of Rad51 And Dmc1 During Meiotic Recombination, Stephen L. Gasior

Biological Sciences Faculty Publications

Two RecA homologs, Rad51 and Dmc1, assemble as cytologically visible complexes (foci) at the same sites on meiotic chromosomes. Time course analysis confirms that co-foci appear and disappear as the single predominant form. A large fraction of co-foci are eliminated in a red1 mutant, which is expected as a characteristic of the interhomolog-specific recombination pathway. Previous studies suggested that normal Dmc1 loading depends on Rad51. We show here that a mutation in TID1/RDH54, encoding a RAD54 homolog, reduces Rad51-Dmc1 colocalization relative to WT. A rad54 mutation, in contrast, has relatively little effect on RecA homolog foci except when strains also …

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Religiopoiesis, Ursula Goodenough Sep 2000

Religiopoiesis, Ursula Goodenough

Biology Faculty Research

Religiopoiesis describes the crafting of religion, a core activity of humankind. Each religion is grounded in its myth, and each myth includes a cosmology of origins and destiny. The scientific worldview coheres as such a myth and calls for a religiopoietic response. The difficulties, opportunities, and imperatives inherent in this call are explored, particularly as they impact the working scientist.

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Regional Variation In Recruitment Of Hemlock Seedlings And Saplings In The Upper Great Lakes, Usa, Thomas P. Rooney, Ronald J. Mccormick, Stephen L. Solheim, Donald M. Waller Aug 2000

Regional Variation In Recruitment Of Hemlock Seedlings And Saplings In The Upper Great Lakes, Usa, Thomas P. Rooney, Ronald J. Mccormick, Stephen L. Solheim, Donald M. Waller

Biological Sciences Faculty Publications

Mature eastern hemlock–northern hardwood forest cover decreased drastically in the upper Midwest following European settlement and has yet to rebound substantially. Previous studies show that stands retaining substantial hemlock canopy coverage have low hemlock seedling and sapling densities. Results from various geographically restricted studies suggest several possible mechanisms that could cause low seedling or sapling density. We examined the relative importance of these proposed mechanisms in the Southern Superior Uplands Section of the Laurentian Forest Province. We surveyed 294- m2 plots in 100 hemlock stands in northern Wisconsin and western upper Michigan to assess how these proposed mechanisms affect the …

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Aintegumenta Promotes Petal Identity And Acts As A Negative Regulator Of Agamous, Beth A. Krizek, Valerie Prost, Anthony Macias Aug 2000

Aintegumenta Promotes Petal Identity And Acts As A Negative Regulator Of Agamous, Beth A. Krizek, Valerie Prost, Anthony Macias

Faculty Publications

The Arabidopsis AINTEGUMENTA (ANT) gene has been shown previously to be involved in ovule development and in the initiation and growth of floral organs. Here, we show that ANT acts in additional processes during flower development, including repression of AGAMOUS (AG) in second whorl cells, promotion of petal epidermal cell identity, and gynoecium development. Analyses of ap2-1 ant-6 double mutants reveal that ANT acts redundantly with AP2 to repress AG in second whorl cells. The abaxial surface of ant petals contains features such as stomata and elongated, interdigitated cells that are not present on wild-type petals. …

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Chromatin Organization And Transcriptional Control Of Gene Expression In Drosophila, G Farkas, B Leibovitch, Sarah C.R. Elgin Aug 2000

Chromatin Organization And Transcriptional Control Of Gene Expression In Drosophila, G Farkas, B Leibovitch, Sarah C.R. Elgin

Biology Faculty Research

It is increasingly clear that the packaging of DNA in nucleosome arrays serves not only to constrain the genome within the nucleus, but also to encode information concerning the activity state of the gene. Packaging limits the accessibility of many regulatory DNA sequence elements and is functionally significant in the control of transcription, replication, repair and recombination. Here, we review studies of the heat-shock genes, illustrating the formation of a specific nucleosome array at an activatable promoter, and describe present information on the roles of DNA-binding factors and energy-dependent chromatin remodeling machines in facilitating assembly of an appropriate structure. Epigenetic …

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The Role Of Nf-Кb Activation In Breast Carcinoma And Glioblastoma Cell Lines, Kelly T. Murphy Aug 2000

The Role Of Nf-Кb Activation In Breast Carcinoma And Glioblastoma Cell Lines, Kelly T. Murphy

Masters Theses

The nuclear factor-КB (NF-КB)/Rel family of inducible dimeric transcription factors regulates numerous genes associated with the immune, mitogenic, and apoptotic responses. Investigations were made into the role of NF-КB in breast carcinoma (MCF-7, T47D, MDA-MB-231) and glioblastoma (U87MG, U373MG, Ul 18MG) cell lines. NF-КB binding to DNA was quantified using both gel mobility shift assays and transient transfections of a vector containing a luciferase gene under the control of 4 tandem copies of the NF-КB binding sequence. To determine the correlation between NF-КB activation, cellular proliferation, and foci formation, the tumor cells were cultured and analyzed in the presence of …

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Isolation And Characterization Of A Genetic Locus That Confers Tolerance To Cadmium And Zinc In Pseudomonas Fluorescens, Szi Fei Feng Aug 2000

Isolation And Characterization Of A Genetic Locus That Confers Tolerance To Cadmium And Zinc In Pseudomonas Fluorescens, Szi Fei Feng

Masters Theses

This thesis describes the isolation and characterization of a region containing several genes involved in metal tolerance in the common soil bacterium, Pseudomonas fluorescens ATCC 13525. The DNA region was identified in a cadmium and zinc sensitive mutant, 45-B10, which contains a lacZ-based reporter gene transposon Tn5-B20 inserted in the chromosome. The DNA surrounding the transposon was cloned from this mutant strain and DNA sequencing of this region revealed seven genes that share similarity with genes whose products are involved in the cation-proton antiporter efflux system of other Gram-negative bacteria. Complete DNA sequence was obtained from four of these …

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Uncharged Trna Activates Gcn2 By Displacing The Protein Kinase Moiety From A Bipartite Trna-Binding Domain, Jinsheng Dong, Hongfang Qiu, Minerva Garcia-Barrio, James T. Anderson, Alan G. Hinnebusch Aug 2000

Uncharged Trna Activates Gcn2 By Displacing The Protein Kinase Moiety From A Bipartite Trna-Binding Domain, Jinsheng Dong, Hongfang Qiu, Minerva Garcia-Barrio, James T. Anderson, Alan G. Hinnebusch

Biological Sciences Faculty Research and Publications

Protein kinase GCN2 regulates translation in amino acid–starved cells by phosphorylating eIF2. GCN2 contains a regulatory domain related to histidyl-tRNA synthetase (HisRS) postulated to bind multiple deacylated tRNAs as a general sensor of starvation. In accordance with this model, GCN2 bound several deacylated tRNAs with similar affinities, and aminoacylation of tRNAPhe weakened its interaction with GCN2. Unexpectedly, the C-terminal ribosome binding segment of GCN2 (C-term) was required in addition to the HisRS domain for strong tRNA binding. A combined HisRS+C-term segment bound to the isolated protein kinase (PK) domain in vitro, and tRNA impeded this interaction. An activating mutation …

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High Efficiency Separation Of Microbial Aggregates Using Capillary Electrophoresis, J. M. Schneiderheinze, Daniel W. Armstrong, G. Schulte, David J. Westenberg Aug 2000

High Efficiency Separation Of Microbial Aggregates Using Capillary Electrophoresis, J. M. Schneiderheinze, Daniel W. Armstrong, G. Schulte, David J. Westenberg

Chemistry Faculty Research & Creative Works

Recent advances in the technique of capillary electrophoresis have demonstrated fast, highly efficient separation of mixtures of intact microbes. This paper describes the application of this technique for the separation of microbial aggregates of Micrococcus luteus, Saccharomyces cerevisiae, or Alcaligenes faecalis. The aggregates of these microbes were resolved into several highly efficient peaks with analysis times under 10 min and efficiencies approaching 1 000 000 plates m-1 in some cases. A reproducible relationship was found between the electrophoretic mobility and the aggregation number or size of the cluster under a given set of experimental conditions. Often, cellular aggregation was reversible …

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Physiology Of A Microgravity Environment Invited Review: Microgravity And Skeletal Muscle, Robert H. Fitts, Danny R. Riley, Jeffrey J. Widrick Aug 2000

Physiology Of A Microgravity Environment Invited Review: Microgravity And Skeletal Muscle, Robert H. Fitts, Danny R. Riley, Jeffrey J. Widrick

Biological Sciences Faculty Research and Publications

Spaceflight (SF) has been shown to cause skeletal muscle atrophy; a loss in force and power; and, in the first few weeks, a preferential atrophy of extensors over flexors. The atrophy primarily results from a reduced protein synthesis that is likely triggered by the removal of the antigravity load. Contractile proteins are lost out of proportion to other cellular proteins, and the actin thin filament is lost disproportionately to the myosin thick filament. The decline in contractile protein explains the decrease in force per cross-sectional area, whereas the thin-filament loss may explain the observed postflight increase in the maximal velocity …

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Early Instar Growth And Survivorship In The Common Baskettail Dragonfly Epitheca Cynosura (Anisoptera: Corduliidae)., Bryan Arthur Reece Aug 2000

Early Instar Growth And Survivorship In The Common Baskettail Dragonfly Epitheca Cynosura (Anisoptera: Corduliidae)., Bryan Arthur Reece

Electronic Theses and Dissertations

Egg masses of Epitheca cynosura were collected from Bays Mountain Park, Tennessee, USA, in June, 1999. Newly hatched individuals were placed into enclosures and sampled at scheduled time intervals throughout the summer. Enclosures were exposed to combinations of high and low densities and presence/absence of a second-year class E. cynosura predator. Survivorship, mean head widths, and mean dry masses were compared across treatments. Due to poor recovery of early-instar larvae, survivorship showed no significant differences in mortality among treatments. The predator present treatment caused significantly smaller head widths and dry masses only on days 42 and 55. The density treatment …

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The Impact Of Lianas On Tree Regeneration In Tropical Forest Canopy Gaps: Evidence For An Alternative Pathway Of Gap‐Phase Regeneration, Stefan A. Schnitzer, James W. Dalling, Walter P. Carson Aug 2000

The Impact Of Lianas On Tree Regeneration In Tropical Forest Canopy Gaps: Evidence For An Alternative Pathway Of Gap‐Phase Regeneration, Stefan A. Schnitzer, James W. Dalling, Walter P. Carson

Biological Sciences Faculty Research and Publications

1 Regeneration in forest canopy gaps is thought to lead invariably to the rapid recruitment and growth of trees and the redevelopment of the canopy. Our observations, however, suggest that an alternate successional pathway is also likely, whereby gap‐phase regeneration is dominated by lianas and stalled in a low‐canopy state for many years. We investigated gap‐phase regeneration in an old‐growth tropical forest on Barro Colorado Island (BCI) in Panama to test the following two hypotheses: (i) many gaps follow a pathway in which they remain at a low canopy height and are dominated by lianas and (ii) the paucity of …

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Physiology Of A Microgravity Environment Invited Review: Microgravity And Skeletal Muscle, Robert H. Fitts, Danny R. Riley, Jeffrey J. Widrick Aug 2000

Physiology Of A Microgravity Environment Invited Review: Microgravity And Skeletal Muscle, Robert H. Fitts, Danny R. Riley, Jeffrey J. Widrick

Biological Sciences Faculty Research and Publications

Spaceflight (SF) has been shown to cause skeletal muscle atrophy; a loss in force and power; and, in the first few weeks, a preferential atrophy of extensors over flexors. The atrophy primarily results from a reduced protein synthesis that is likely triggered by the removal of the antigravity load. Contractile proteins are lost out of proportion to other cellular proteins, and the actin thin filament is lost disproportionately to the myosin thick filament. The decline in contractile protein explains the decrease in force per cross-sectional area, whereas the thin-filament loss may explain the observed postflight increase in the maximal velocity …

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Kentucky Salamanders Of The Genus Desmognathus: Their Identification, Distribution, And Morphometric Variation, Leslie Scott Meade Jul 2000

Kentucky Salamanders Of The Genus Desmognathus: Their Identification, Distribution, And Morphometric Variation, Leslie Scott Meade

Morehead State Theses and Dissertations

A thesis presented to the faculty of the College of Science and Technology at Morehead State University in partial fulfillment of the requirements for the Degree of Master of Science in Biology by Leslie Scott Meade on July 24, 2000.

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Direct Determination Of The Kinetics Of Oxygen Diffusion To The Photocytes Of A Bioluminescent Elaterid Larva, Measurement Of Gas- And Aqueous-Phase Diffusional Barriers And Modelling Of Oxygen Supply, G. S. Timmins, E. J. Bechara, H. M. Swartz Jul 2000

Direct Determination Of The Kinetics Of Oxygen Diffusion To The Photocytes Of A Bioluminescent Elaterid Larva, Measurement Of Gas- And Aqueous-Phase Diffusional Barriers And Modelling Of Oxygen Supply, G. S. Timmins, E. J. Bechara, H. M. Swartz

Dartmouth Scholarship

We describe the development and use of a direct kinetic technique to determine the time taken for oxygen to diffuse from the external environment into the light-producing cells (photocytes) in the prothorax of bioluminescent larvae of Pyrearinus termitilluminans. This was achieved by measuring the time course of the pseudoflash induced through sequential anoxia followed by normoxia. We have also determined the separate times taken for this oxygen diffusion in gaseous and tissue (predominantly aqueous) phases by using helium and nitrogen as the carrier gas. Of the total time taken for diffusion, that in the gas phase required 613+/-136 ms (mean …

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Molecular Aspect Of Leaf Senescence, Edward Himelblau, Betania Quirino, Yoo-Sun Noh, Richard Amasino Jul 2000

Molecular Aspect Of Leaf Senescence, Edward Himelblau, Betania Quirino, Yoo-Sun Noh, Richard Amasino

Biological Sciences

Senescence is the last stage of leaf development and one type of programmed cell death that occurs in plants. The relationships among senescence programs that are induced by a variety of factors have been addressed at a molecular level in recent studies. Furthermore, an overlap between the pathogen-response and senescence programs is beginning to be characterized. The complexity of the senescence program is also evident in studies of senescence-specific gene regulation and the role of photosynthesis and plant hormones in senescence regulation. New molecular-genetic approaches are expected to be useful in unraveling the molecular mechanisms of the leaf senescence program.

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Sequence Analysis Of Bacterial Dna In The Colon And Stomach Of The Tyrolean Iceman, Raul J. Cano, Friedrich Tiefenbrunner, Massimo Ubaldi, Clarissa Del Cueto, Stefania Luciani, Tobe Cox, Paula Orkand, Karl H. Kunzel, Franco Rollo Jul 2000

Sequence Analysis Of Bacterial Dna In The Colon And Stomach Of The Tyrolean Iceman, Raul J. Cano, Friedrich Tiefenbrunner, Massimo Ubaldi, Clarissa Del Cueto, Stefania Luciani, Tobe Cox, Paula Orkand, Karl H. Kunzel, Franco Rollo

Biological Sciences

The male human body found in an Alpine glacier on September 19, 1991 ("Tyrolean Iceman") has, for the first time in history, given scientists a chance to perform detailed anatomical, histological, and molecular investigations on the organs of a person from the Neolithic Age (5350-5100 B.P.). In the present study, tissue samples aseptically taken from the stomach and the colon of the mummy were utilized for DNA extraction, and the DNA was PCR-amplified, using primer pairs designed to bind to fragments of the 16s ribosomal RNA gene (16s rDNA) of a broad range of bacteria. The PCR products were cloned …

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