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Articles 31 - 58 of 58
Full-Text Articles in Biology
Effects Of Various Proportions Of Different Additive Substances On Themiselle Growth Of Pleurotus Florida Fovose, On Druationof Formation And Development Of Basidiocarps And On The Yield, Abdunnasır Yildiz
Turkish Journal of Biology
Into aliment place that we prapered for vegetative propagation of the miscell, in 1 lt pure water 10 g malt-extract, 0.2 g K 2 HPO 4 , 0.1 g CaCl 2 .2H 2 O 0.05 g MgSO 4 . 7H 2 O, and g (NH 4 )SO 4 as well as 15 g agar, barley broken and wheat flour in 10, 15 and 20 g doses were added as contribution substance (contribution substance, however, weren't added to the control group). In petri boxes, it has been identified that P. florida miscelles grafted for aliment-agar medium covered the medium in 8.6 …
The Construction Of A Mammalian Transfectionvector For Expression Of Cytosine-5 Specific Dnamethyltransferase Gene M.Msp1 In Cultured Cells, Öztürk Özdemi̇r
The Construction Of A Mammalian Transfectionvector For Expression Of Cytosine-5 Specific Dnamethyltransferase Gene M.Msp1 In Cultured Cells, Öztürk Özdemi̇r
Turkish Journal of Biology
The expression vectors are designed for expression and purification of normal or recombi-nant gene of interest. There is a wide variety of stable and transferable selectable mammalian expres-sion vectors.The vector pCDM8 and its derivatives, pcDNAI/Ampicilline are widely used for cloning and analyzing of genes in higher eukaryotic cells. The vectors pRC/CMV, pcDNA3 and pRC/RSV are designed for high-level expression of recombinant genes in mammalian cells. In the present study, we have been able to transfect and express the monospecific bacterial (Moroxcella spp) cytosine-5 DNA methyltransferase (MTase) M.Msp1 gene in cultured cells within a newly con-structed mammalian transfection vector. We have …
Serological Properties Of Pseudomonas Syringaepvphaseolicolaisolates Collected From Eskisehir, Dilek Ertugrul, Kıymet Güven
Serological Properties Of Pseudomonas Syringaepvphaseolicolaisolates Collected From Eskisehir, Dilek Ertugrul, Kıymet Güven
Turkish Journal of Biology
Thirty six bean seed samples collected from bean fields in Eskisehir-Türkiye were examined for detection and isolation of Pseudomonas syringae pv. phaseolicola. A polyclonal antiserum specific to P. s. pv. phaseolicola NCPPB 52 was raised in a rabbit and both biochemical and serological test were used for identification. Heat-stable O antigens of 92 isolates identified as P. S. pv. phaseolicola together with authentic isolates of the bacterium were compared by Ouchterlony immunodiffusion test and all the isolates had a common antigenic structure.
Purification And Characterization Of Pediocin F, Abacteriocin Produced By Pediococcus Acidilacticif, Özlem Osmanagaoğlu, Ufuk Gündüz, Yavuz Beyatli, Cumhur Çökmüş
Purification And Characterization Of Pediocin F, Abacteriocin Produced By Pediococcus Acidilacticif, Özlem Osmanagaoğlu, Ufuk Gündüz, Yavuz Beyatli, Cumhur Çökmüş
Turkish Journal of Biology
In this work, Pediococcus acidilactici F isolated from fermented sausage was reported to produce an extracellular antimicrobial peptide, termed Pediocin F, that was protein in nature and effec-tive against many bacteria associated with food spoilage and food related health hazards. Pediocin F is reported here to be sensitive to proteolytic enzymes, resistant to heat and organic solvents, and active over a wide range of pH. We have also reported that the extracellular antimicrobial protein pro-duced by P. acidilactici F was really a small peptide. Coomassie-blue stained SDS-PAGE technique allowed us to estimate the molecular weight of this small peptide to …
Recent Developments In Molecular Plant-Microbe Interactions, Mahmut Tör
Recent Developments In Molecular Plant-Microbe Interactions, Mahmut Tör
Turkish Journal of Biology
Generally, plants are affected by biotic and abiotic elicitors in nature. These elicitors are recognized by receptors on the plasma membrane and the information is passed to the responsible part of the cell via a signal transduction pathway. In terms of plant-pathogen interaction, the products of avr genes of the pathogens are recognized by the products of R genes of the host plant. Following recognition, the other genes, including kinases, in the cascade are phosphorylated and the defence mechanisms of the plant are activated. As a result, the plant cell commits suicide and the growth of the pathogen is restricted. …
Fine Structure Of A-Type Neurosecretory Cells In Abscisic Acid Treatedlocusta Migratorial. (Orthoptera), Önder Deveci̇
Fine Structure Of A-Type Neurosecretory Cells In Abscisic Acid Treatedlocusta Migratorial. (Orthoptera), Önder Deveci̇
Turkish Journal of Biology
Fine structure of A-type neurosecretory cells was investigated in the adult males of Locusta migratoria in solitary phase. The cells were surrounded by glial cell processes and contained electron dense granules of 257 nm in an average diameter. Most of the granules were alongated but some were spherical. In control group insects, regular narrow channels of rough surfaced endoplasmic reticulum were evident in the peripheral parts of the cells. Golgi complexes were observed with electron dense material within its vacuoles and vesicles. The number of stored granules were not abundant and large empty areas were observed in the cells. In …
Effects Of Salt And Exogenous Putrescine On Seed Germination And Earlyseedling Growth Of Helianthus Annuusl. Var. Santafe, Fatma Teki̇n, Suna Bozcuk
Effects Of Salt And Exogenous Putrescine On Seed Germination And Earlyseedling Growth Of Helianthus Annuusl. Var. Santafe, Fatma Teki̇n, Suna Bozcuk
Turkish Journal of Biology
Effects of salt (NaCl) and putrescine (Put.) and various combinations of these (salt+Put.) on germination and some early growth parameters of sunflower ( Helianthus annuus L. var. Santafe) plants, grown under the controlled conditions, were investigated. Salt was used in 3 different con-centrations (50, 100, 200mM) and also, Put. was applied in 3 levels (0.01, 1, 2 mM). The salt treat-ment alone inhibited or delayed the seed germination, depending on concentration. Furthermore, some growth parameters (length of radicle, fresh and dry weights) studied in germination period were adversely affected by salt. In the treatment alone of Put., regardless of the …
The Influence Of Biology Education On Attitudes To Humanity And Worldpeace, Yalçın Yetki̇n
The Influence Of Biology Education On Attitudes To Humanity And Worldpeace, Yalçın Yetki̇n
Turkish Journal of Biology
The purpose of this study was to determine whether biology education has any effect on behaviour and opinions with regard to humanity and world peace. The subjects were 37 student volunteers, 19 male and 18 female, from the Biology Department of Atatürk University. They were asked what influence biology education has on individual peace of mind and attitudes to world peace. The students responses indicated that, owing to their biology education, they have greater self-awareness (46.9%), are better able to think in a balanced way and to find their place in society. They also reported a greater capacity to love …
The Effects Of Copper Chloride On The Gene Activity Of The Salivarygland Polytene Chromosomes Of Drosophila Melanogaster, Sevgi Levent, Handan Uysal, Zafer Bahçeci̇
The Effects Of Copper Chloride On The Gene Activity Of The Salivarygland Polytene Chromosomes Of Drosophila Melanogaster, Sevgi Levent, Handan Uysal, Zafer Bahçeci̇
Turkish Journal of Biology
The effects of copper chloride on the salivary gland polytene chromosomes of third instar larvae of Drosophila melanogaster have been investigated. Copper chloride have been applied to the larvae in vivo. It has been shown that different band sections of the salivary gland chromosomes become puffed and many developmental puffs were inactivated. As a result of the copper chloride treatment, band 22A, 23EF, 27E, 28B, 35F on chromosome arm 2L; 47A, 54BD, 55DE, 56DE, 58CF, 59B on chromosome arm 2R; 62A, 62DE, 68BC, 70DE, 73B, 74D on chromosome arm 3L have become puffed. The average size of these puff have …
The Targeting Mechanisms Of Membrane Proteins, Nihat Di̇lsi̇z
The Targeting Mechanisms Of Membrane Proteins, Nihat Di̇lsi̇z
Turkish Journal of Biology
This review describes what is known about the way of insertion and translocation of membrane proteins and the factors that determine these structures. The translocation of proteins across the cytoplasmic membrane in prokaryotes and that across the endosplasmic reticulum membrane in eukaryotes are similar in that both require the sinyal peptide that is attached to the amino terminus of the proteins. In general, mebrane proteins reach their final target via the secretory (Sec) pathway which are called Sec-dependent proteins. However, it has become clear that a few membrane proteins are not involved in Sec-pathway and these are called Sec-independent proteins.
The Algal Flora Of The Littoral Region In Lake Akşehir, Ayşe Elmaci, Olcay Obali
The Algal Flora Of The Littoral Region In Lake Akşehir, Ayşe Elmaci, Olcay Obali
Turkish Journal of Biology
In this study, composition and seasonal variations of littoral algae in Lake Akşehir were investigated from June 1992- November 1993. In the littoral region, the algal flora were composed of 115 species belonging to Bacillariophyta, Charophyta, Chlorophyta, Cyanophyta, Euglenophyta. Bacillariophyta was predominant in respect to species diversity and density. Cyanophyta and Chlorophyta were in minority. In general, the most of the identified species of the algal flora of Lake Akşehir are considered as the members of eutrophic lakes which are rich in nutritional salts.
Transcription Of The Autographa Californicanuclear Polyhedrosis Virus P39 Gene In Bombyxmori Cell Culture, Zihni Demi̇rbağ
Transcription Of The Autographa Californicanuclear Polyhedrosis Virus P39 Gene In Bombyxmori Cell Culture, Zihni Demi̇rbağ
Turkish Journal of Biology
Transcription of the Autographa californica nuclear polynedrosis virüs (AcNPV; Family: Baculoviridae) p39 gene (delayed-early gene) has been studied extensively in the productive system of Spodoptera frugiperda cells. This is the first transcriptional study of this gene in the Bombyx mori cell line. In this study, comparative transcription of the AcNPV p39 gene was examined in S. frugiperda and B. mori cell cultures. Northern blot hybridization of totoal RNA samples, isolated from mock-infected (O and 48 hour post infection, h.p.i.) and AcNPV-infected (0, 2, 6, 12, 24, 48 h.p.i.) S. frugiperda and B. mori cell lines, was analyzed. Results indicates that …
Determination Of Heavy Metal Levels In Some Mossspecies Around Thermic Power Stations, Özlem Tonguç
Determination Of Heavy Metal Levels In Some Mossspecies Around Thermic Power Stations, Özlem Tonguç
Turkish Journal of Biology
In our studry, the chemical analysis related to the heavy metal pollution in some mosses grown near the thermic power stations which built close to Mugla and its environs, was carried out.
A Comparison Of Germ Tube Production By Candidaalbicans In Three Media, Gülhan Vardar-Ünlü
A Comparison Of Germ Tube Production By Candidaalbicans In Three Media, Gülhan Vardar-Ünlü
Turkish Journal of Biology
The conversion of C. albicans. from commensal to the pathogenic state is associated with its ability to make the dimorphic transition from the yeast to the hyphal (germ tube) form. Three liq-uid media were compared in ability to promote germ tube production by C. albicans. Medium 199 and Glucose-Glycine Medium at 37°C were highly satisfactory and gave 90 % conversion of yeast cells to hyphal-form cells. Sucrose-Gelatine medium gave only 15% conversion. Glucose-Glycine medium appeared to give more rapid germ tube production than 199 Medium.
Fatty Acid Composition In Phospholipid And Triacylglycerol Fractions Ofmelanogryllus Desertuspall. (Orthoptera: Gryllidae), Mehmet Başhan
Fatty Acid Composition In Phospholipid And Triacylglycerol Fractions Ofmelanogryllus Desertuspall. (Orthoptera: Gryllidae), Mehmet Başhan
Turkish Journal of Biology
In this study, the fatty acid compositions in phospholipid and triacylglycerol factions of ten-day- old adults of both sexes of Melanogryllus desertusreared on stock culture medium under labora-tory conditions, and the total fatty acid composition of the stock culture medium were analysed sep-arately by gas chromatography. Quantitative analysis of both fractions and the stock culture medium showed that oleic, palmitic and linoleic acids constituted the major part of the fatty acid compositions. The fatty acid compositions of the phospholipid and triaclglycerol fractions were quantitatively different. Moreover, a polyunsaturat-ed fatty acid. linolenic acid and eicosapentaenoic acids which is the precursor of …
Investigation Of Mold Flora In Moldy Cheeses Of Konya And Itsvicinity, Birol Özkalp, Yusuf Durak
Investigation Of Mold Flora In Moldy Cheeses Of Konya And Itsvicinity, Birol Özkalp, Yusuf Durak
Turkish Journal of Biology
In this study, 140 moldy cheese samples were investigated in view of mold flora. 9 microfungi species which belong to Penicillium and Aspergillus genera were isolated from moldy cheese samples. 87.16% of isolates were Penicillium and 12.84% were Aspergillus species. P. ro-queforti were determined as dominant species in all samples.
The Effect Of Some Plant Materials On The Grwth And Productivity Ofpleurotus Ostreatus (Jacq. Ex. Fr.) Kum. Var. Salignus (Pers. Ex. Fr.)Konr. Et Maubl., Abdunnasır Yildiz, Ramazan Demi̇r
The Effect Of Some Plant Materials On The Grwth And Productivity Ofpleurotus Ostreatus (Jacq. Ex. Fr.) Kum. Var. Salignus (Pers. Ex. Fr.)Konr. Et Maubl., Abdunnasır Yildiz, Ramazan Demi̇r
Turkish Journal of Biology
In this study, the soybean, sorghum, peanut and wheat straw were used as culture media for Pleurotus ostreatus var. salignus. Mycellium growth period, basidiocarp formation period anda first, second, third and fourth harvesting periods were determined as; 10.0, 24.3, 28.6 38.6, 47.3 and 58.6 days respectively as the shortest periods found with peanut straw; and 22.6, 52.6, 56.6, 68.6, 73.6 and 88.6 days respectively, as the longest periods found with sorghum straw. The fresh mushroom yield obtained with 100 g material (70 % moisture) at first, second, third and fourth harwesting periods and total yield was 8.6, 8.1, 4.8, 3.4 …
Fatty Acid Composition Of Agaricus Bisporus (Lange) Sing., Abdurrahman Aktümsek, Celâleddin Öztürk, Giyasettin Kaşik
Fatty Acid Composition Of Agaricus Bisporus (Lange) Sing., Abdurrahman Aktümsek, Celâleddin Öztürk, Giyasettin Kaşik
Turkish Journal of Biology
Fatty acid compositions of fruit body, stem, lamellae and total of Agaricus bisporus were seperately analysed by GLC. In the all fatty acid compositions of A. bisporus, linoleic acid were predominant. Percentages of linoleic acid were varied between 53.45 - 68.78%. It was showed that the other major fatty acids were palmitic, oleic and stearic acid in the fatty acid compositions.
Antimicrobial Activity Of The Lichen Cetraria Islandica (L.) Ach., Başaran Dülger, Fahrettin Güci̇n, Ali Aslan
Antimicrobial Activity Of The Lichen Cetraria Islandica (L.) Ach., Başaran Dülger, Fahrettin Güci̇n, Ali Aslan
Turkish Journal of Biology
In this study, extracts of Cetraria islandica (L.) Ach. were prepared with Ethyl acetate, Acetone, Chloroform and Ethanol and antimicrobial activities of these extracts were examined on test microorganisms as follows: Escherichia coli ATCC 11230, Enterobacter aerogenes CCM 2531, Staphylococcus aureus 6538P, Staphylococcus epidermidis, Bacillus subtilis La2114, Bacillus cereus var. mycoides, Bacillus sphaericus, Bacillus thurigiensis, Bacillus megaterium, Mycobacterium smegmatis RUT, Salmonella thyphimurium, Candida utilis La991, Candida albicans CCM314, Aspergillus flavus, Aspergillus oryzae, Aspergillus fumigatus, Trichophyton rubrum, Botrytis cineriae, Fusarium oxysporium, Streptomyces murinus ISP 5091 and Nocardia cornea IFO 14403 by Disc Diffusion Methods. As a result of study, we have …
Biochemical Characterization Of Elastase Frompseudomonas Aeruginosa Ses 938-1, Semra Kocabiyik, Ezgi Ergi̇n
Biochemical Characterization Of Elastase Frompseudomonas Aeruginosa Ses 938-1, Semra Kocabiyik, Ezgi Ergi̇n
Turkish Journal of Biology
We have investigated some biochemical properties of elastase from a new strain o f Pseudomonas aeruginosa (SES 938-1). Activities were measured at 410 nm using N-succinyl-L-(ala) 3 -p- nitroanilide as substrate. The elastase activity followed Michaelis-Menten kinetics over the substrate range of 0.067-0.540 mM with the apparent K m value of 0.375 mM. Optimum activity was observed at 36 °C and pH 7.5. elastase activity was inhibited by metal chelating agents and high concentrations of Mn 2+ , Zn 2+ and Ni 2+ . The results obtained suggest that elastase from P. aeruginosa SES 938-1 is a neutral metalloproteinase.
The Effects Of Phenoxybenzamine And Cold Stresson Tyrosine Hydroxylase Enzyme Activity Andexpression Of Tyrosine Hydroxylase Gene In The Ratadrenal Medulla, Muhittin Yürekli
The Effects Of Phenoxybenzamine And Cold Stresson Tyrosine Hydroxylase Enzyme Activity Andexpression Of Tyrosine Hydroxylase Gene In The Ratadrenal Medulla, Muhittin Yürekli
Turkish Journal of Biology
The effects of cold exposure and cold plus phenoxybenzamine treatment on tyrosine hydrox-ylase (TH) activity and TH m RNA levels were investigated in the adrenal medulla of Sprague Dawley (SD) rats. SD rats were exposed to cold (4 °C) for 48 hours. Phenoxybenzamine was injected intra-peritoneally (i.p) at 20 mg/kg, which was prepared in 5% ethanol containing 0.9 % NaCL TH activity was measured by monitoring the formation of 3 H 2 O as a product of L-(3 H)-tyrosine hydroxylation, the formation of L-(3 H)-dopa from L-(3 H)-tyrosine. Total adrenal RNA was isolated and blotted onto a nylon membrane and …
A Theoretical Study On The Sex-Pheromones Of Therice Moth, Corcyra Cephalonica Stainton, Lemi Türker
A Theoretical Study On The Sex-Pheromones Of Therice Moth, Corcyra Cephalonica Stainton, Lemi Türker
Turkish Journal of Biology
Molecular orbital calculations (AM1 type) were performed on the geometry optimized structures of various farnesal isomers and methyl esters of farnesoic acids. It has been found that the major component, E, E-farnesal, of the sex-pheromone for the rice moth, Corcyra cephalonica Stainton is chemically the most exothermic and stable of all the farnesal isomers. Then, the resem-blance of molecular shapes was detrmined in order to design more stable sex-attractants.
Larval Growth And Survival Of Penaeus Indicus(Decapoda: Penaeidae) On Live Feeds, Metin Kumlu
Larval Growth And Survival Of Penaeus Indicus(Decapoda: Penaeidae) On Live Feeds, Metin Kumlu
Turkish Journal of Biology
This study was conducted to compare the suitability of three microalgal species (Tetraselmis chuii, Skeletonema costatum and Rhinomonas reticulata), both individually and in combination, as food for Penaeus indicus during larval culture. To determine the best feeding density, each alga was fed to protozoea 1 (PZI) larvae from 10 to 80 cells mL -1 . The best algal-cell density, promoting the highest survival rate, greatest growth and fastest larval development, was obtained at 60-70 cells mL -1 with a combination of T. chuii and S. costatum. Low cell densities (10-20 cells mL -1 ) were not effective. The diatom S. …
The Effect Of 9450 Mhz Microwave Radiation On The Chromosomes Invivo, M. Zülküf Akdağ, Cemil Sert, M. Salih Çeli̇k, M. Emin Erdal, M. Aydın Ketani
The Effect Of 9450 Mhz Microwave Radiation On The Chromosomes Invivo, M. Zülküf Akdağ, Cemil Sert, M. Salih Çeli̇k, M. Emin Erdal, M. Aydın Ketani
Turkish Journal of Biology
In this study, we investigated whether microwave radiation affected chromosomal structure of rats or not. Adult male Spraque-Dawley rats were irradiated for 1 h a day to continous micorwave radiation (9450 MHz) at powever density 2.65 mW/cm 2 during 13. days. The control group were exposed to the some procedurus without microwave irradiation. On the following exposure day, rats were sacrificed under cetalar anesthesia, and their femurs were removed. Satandard bone marrow chromosome analysis were caried out, and experimental group was compared with control group. Our results showed that abnormal metaphase count increased and mitotic index decreasad, therefor, after and …
In Vivo Dna Methylation Of Escherichia Colidh5\Alphaand Top10f' Strains By Bacterial Cytosine-5methyltransferase M. Msp1, Öztürk Özdemi̇r, David Hornby
In Vivo Dna Methylation Of Escherichia Colidh5\Alphaand Top10f' Strains By Bacterial Cytosine-5methyltransferase M. Msp1, Öztürk Özdemi̇r, David Hornby
Turkish Journal of Biology
At the chromatin level, methylated CpG dinucleotides are R.Msp1 resistant compared with nonmethylated counterparts. The DNA of two E.coli strains was analyzed following transformation-with bacterial cytosine-5-methyltransferase gene M.Msp1 in mammalian transfection vector pcDNA3. Expression of the M.Msp1 was tested by R.Msp1 digestion. The results suggest that the DNA of both strains was fully methylated at the CCGG sequences, by the active enzyme under the control of T7 and cytomegalovirus (CMV) promoters. Methylated DNA can not be digested and it exhibits higher frag-ment sizes in 1% agarose gel in contrast to the untransformed cell DNAs in vivo.
Determination Of 5'-Nucleotidase Activity Of Higherplants By Using An Improved Method, Yusuf Turan
Determination Of 5'-Nucleotidase Activity Of Higherplants By Using An Improved Method, Yusuf Turan
Turkish Journal of Biology
The presence of relatively high non-specific phosphatase activities are known in various plant tissues. These enzymes also catalyze the removal of inorganic phosphate from nucleoside monophosphates. According to published reports, so far, the experimental investigations, concerning the 5'-nucleotidase activity, have been mostly carried out without consideration of the non-specific phosphatase activities in crude enzymic extracts. However, few researchers have been included some inhibitors of these phosphatases, such as NaMoO 4 or KF, in assay mixtures. But phosphatases are not completely inhibited by these inhibitors and, therefore, interfere the activity of 5'-nucleotidase. Thus, the non-specific phosphatase activities were determined in crude …
Flora Hellenica, Adi̇l Güner
Use Of Baculovirus As Expression Vector In Biotechnology, Zihni Demi̇rbağ, A. Osman Beldüz, İsmail Demi̇r
Use Of Baculovirus As Expression Vector In Biotechnology, Zihni Demi̇rbağ, A. Osman Beldüz, İsmail Demi̇r
Turkish Journal of Biology
Variuos viral, bacterial, plant and animal proteins which are important for medicinal, industrial and agricultural aspects, have been synhesized via expression vector systems have great importance in biotechnology. Baculoviruses, used as a good model system in molecular biology studies, a biological control agent in agriculture and an expression vector for synthesis of important proteins, opened up a significant period for recombinant DNA technology and protein production. Desired foreign genes, code for various products such as drug, toxin and nutrient substances, can be replaced with viral genes which are not essasial for replication of baculovirus in cell culture. Priority and advantages …