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The Construction Of A Mammalian Transfectionvector For Expression Of Cytosine-5 Specific Dnamethyltransferase Gene M.Msp1 In Cultured Cells, Öztürk Özdemi̇r
The Construction Of A Mammalian Transfectionvector For Expression Of Cytosine-5 Specific Dnamethyltransferase Gene M.Msp1 In Cultured Cells, Öztürk Özdemi̇r
Turkish Journal of Biology
The expression vectors are designed for expression and purification of normal or recombi-nant gene of interest. There is a wide variety of stable and transferable selectable mammalian expres-sion vectors.The vector pCDM8 and its derivatives, pcDNAI/Ampicilline are widely used for cloning and analyzing of genes in higher eukaryotic cells. The vectors pRC/CMV, pcDNA3 and pRC/RSV are designed for high-level expression of recombinant genes in mammalian cells. In the present study, we have been able to transfect and express the monospecific bacterial (Moroxcella spp) cytosine-5 DNA methyltransferase (MTase) M.Msp1 gene in cultured cells within a newly con-structed mammalian transfection vector. We have …