Biology Commons^™

Enhanced Formulation And Comprehensive Analysis Of Novel Natural Ointments Withgrape Seed And Pomegranate Peel Infused In Olive Oil, Mebarka Imane Benguechoua, Madjda Benguechoua, Khedidja Benarous, Alaeddine Kaouka, Mohamed Yousfi

Turkish Journal of Biology

Background/aim: The global shift toward sustainable and organic alternatives in cosmetics and personal care has created a demandfor innovative and environmentally conscious formulations. This study aimed to develop novel natural ointments using beeswax andgrape seed (GS) and pomegranate peel (PP) extracts infused in extra virgin olive oil (EVOO), and determine the physicochemicalcharacteristics, microbiological properties, and antioxidant activity as a way to valorize organic waste toward its application in thecosmetic industry. The research aimed to promote sustainability and effectiveness in the cosmetic industry.Materials and methods: Microbial analysis was performed on the oils and ointments, followed by the extraction of active compoundsusing …

Determining The Optimal Antioxidant Strategy: A Comparative Analysis Of Extractionmethods In Cistus Villosus L. Extracts Revealed By Lc-Hrms And Computationalmodeling, Hicham Bouakkaz, Ebru Erol, Khedidja Benarous, Gülaçti Topçu, Reguia Mahfoudi, Amar Djeridane, Mohamed Yousfi

Turkish Journal of Biology

Background/aim: Cistus villosus L., a beloved tea herb, contains a treasure trove of potent antioxidants. Our research unveils thisancient ally’s potential to shield against oxidative stress, a modern health threat.Materials and methods: Antioxidant assays were conducted to evaluate the potency of ethyl acetate extract. IC50 values were determinedfor the extract in various antioxidant assays. Furthermore, molecular docking was employed for the examination of the 22 compoundsanalyzed through LC-HRMS. Additionally, ADMET and PASS investigations were conducted to evaluate their capability as antioxidantsand inhibitors of oxidant formation.Results: Each identified compound boasts a history of antioxidant prowess, building the natural defense arsenal of …

The Cumulative Effect Of Ellagic Acid And Carnosic Acid Attenuates Oxidative Events During Diabetic Wound Healing: In Different Applications And On Different Days, Eli̇f Naz Gürsoy, Kanuni̇ Barbaros Balabanli, Fatma Nur Tuğcu Demi̇röz, Şule Coşkun Cevher

Turkish Journal of Biology

Background/aim: The hyperglycemic environment in diabetes disrupts normal wound-healing processes, leading to chronic wounds. This study investigated whether the combination of the phenolic compounds Ellagic acid and Carnosic acid shows synergistic effects on diabetic wound healing and oxidative parameters in diabetic rats.Materials and methods: In this study, diabetic rats were divided into control, untreated, Carbopol 974P treated, topical treatment, and oral gavage treatment. Ellagic acid and Carnosic acid combination were applied topically and oral gavage to the full-thickness excisional wounds of all animals except the control group. We investigated oxidative events by malondialdehyde, glutathione, nitric oxide, protein carbonyl, collagen spectrophotometrically, …

Cannabinoid Receptor Ligands Modulate Fibrosis And Inflammation In Idiopathicpulmonary Fibrosis: A Preliminary Study, Sevi̇l Köse, Seli̇n Önen, Merve Gi̇zer, Esi̇n Boduroğlu, Uğur Gönüllü, Petek Korkusuz

Turkish Journal of Biology

Background/aim: There is no specific pharmacological treatment regimen for idiopathic pulmonary fibrosis (IPF). Therefore, new anti-inflammatory therapeutic strategies are needed. Cannabinoids (CBs) that are known for their inflammation-modulating and anti-fibrotic effects may be potential medication candidates for the treatment of IPF. Our aim is to evaluate the inflammation-modulating and anti-fibrotic effects of CB receptor (CBR) agonists and antagonists in lipopolysaccharide (LPS) stimulated human lung healthy fibroblast, epithelial cells, IPF fibroblast cells and monocytes.Materials and methods: We detected CBRs in the human lung healthy (LL24) and IPF fibroblast cells (LL29), epithelial cells (A549) and monocytes (THP-1) by flow cytometry (FC). We …

Development Of An Immunodiagnostic Assay For The Detection Of Sugarcane Mosaic Virus, Hafiz Muhammad Khalid, Najam Us Sahar Sadaf Zaidi, Naeem Rashid, Muhammad Tahir

Turkish Journal of Biology

Background/Aim: Sugarcane mosaic virus (SCMV; genus Potyvirus and family Potyviridae), poses a significant threat to global sugarcane cultivars, including those in Pakistan. The aim of this study was to develop a rapid and effective diagnostic tool for detection of SCMV, enabling timely implementation of control measures to mitigate potential yield losses.Materials and Methods: The study focused on the in silico analysis; physicochemical properties, immunogenicity and subcellular localization of the SCMV coat protein (CP). The SCMV coat protein (CP) gene was synthesized, cloned and expressed in Escherichia coli. The recombinant fusion coat protein (rFCP-SCMV) was purified and used to generate polyclonal …

Combinational Therapy Of All-Trans Retinoic Acid (Atra) And Sphingomyelin Induces Apoptosis And Cell Cycle Arrest In B16f10 Melanoma Cancer Cell, Zeynep İşlek Köklü, Eli̇f Li̇dya Şanverdi̇, Başak Karadağ, Mehmet Hi̇kmet Üçişik, Ezgi̇ Taşkan, Fi̇kretti̇n Şahi̇n

Turkish Journal of Biology

Background: Melanoma arises from the uncontrolled multiplication of melanocytes, posing an escalating global health concern. Despite early detection and surgical removal being crucial, metastatic melanoma poses treatment challenges, with limited options like chemotherapy and immunotherapy. All-trans retinoic acid (ATRA), a natural metabolite of vitamin A, has shown promise in melanoma treatment by inducing differentiation, apoptosis, growth arrest, and immune modulation. However, ATRA treatment alone can lead to resistance and relapse. Sphingomyelin (SM) also plays a role in inhibiting melanoma progression by apoptotic cell death.Objective: This study investigated combinational anticancer effects of ATRA and SM on in vitro B16F10 melanoma cells.Method: …

Organ-On-A-Chip Platforms For Drug Development, Cellular Toxicity Assessment, And Disease Modelling, Muhammad Maaz Khurram, Göktürk Ci̇nel, Özlem Yeşi̇l Çeli̇ktaş, Erdal Bedi̇r

Turkish Journal of Biology

Organ-on-a-chips (OoCs) or microphysiological platforms are biomimetic systems engineered to mimic organ structures onto microfluidic devices for biomedical research. These microdevices can mimic biological environments that enable cell-cell interactions at a small scale by mimicking 3D in vivo microenvironments outside the body. Thus far, numerous single and multiple OoCs that recapitulate organs have been developed, and they have emerged as forerunners for drug efficacy and cytotoxicity testing. This review explores OoC platforms to portray their versatility in investigating drug safety, efficacy, and toxicity. We also reflect upon the potential of OoCs to effectively demonstrate disease models for probable novel therapeutics, …

Mechanistic Insights Into Cardiac Regeneration And Protection Through Meis Inhibition, Aynura Mammadova, Gali̇p Servet Aslan, Li Mo, Qifang Wu, Sebastian Clauss, Fati̇h Kocabaş

Turkish Journal of Biology

MEIS1, a member of the TALE-type homeobox gene family, has emerged as a pivotal regulator of cardiomyocyte cell cycle arrest and represents a promising therapeutic target. Our study reveals that inhibition of MEIS1 using two novel small molecules, MEISi-1 and MEISi-2, significantly enhances neonatal cardiomyocyte proliferation and cytokinesis. Specifically, MEISi-1 and MEISi-2 increased the proportion of proliferating cardiomyocytes (Ph3+TnnT cells) upto 4.5 fold and the percentage of cytokinetic cardiomyocytes (AuroraB+TnnT cells) by 2 fold, compared to untreated controls. MEIS1 inhibition resulted in a notable downregulation of MEIS1 target genes and cyclin-dependent kinase inhibitors, demonstrating its effect on key regulatory pathways. …

Chelidonic Acid Abrogates Oxidative Stress And Memory Dysfunction In Experimental Aging Rats, Feri̇de Ni̇hal Si̇nan, Emel Serdaroğlu Kaşikçi, Burcu Çevreli̇

Turkish Journal of Biology

Background/aim: In an aging model established in male Wistar albino rats via the administration of D-galactose (D-gal), the aim of this study was to examine the effects of chelidonic acid on cognitive functioning and the levels of glutathione (GSH), malondialdehyde (MDA), Total antioxidant status (TAS), and brain-derived neurotrophic factor (BDNF).Materials and methods: Thirty-two Wistar albino (three month old) male rats (n = 8) were used. Group 1: Control (C), Group 2: Chelidonic acid (CA) (2 mg/kg oral gavage), Group 3: D-galactose (D-gal) (150 mg/kg, s.c.), Group 4: D-gal (150 mg/kg) + CA (2 mg/kg) (DC). Following an overnight fast, the …

Characterization Of Tfiie-Regulated Genes By Transcriptome Analysis, Serdar Baysal

Turkish Journal of Biology

RETRACTED: This article has been retracted. Please see the Retraction Notice for details. An Expression of Concern was previously published.

Previous studies on GTF2E showed that it has association with certain group of diseases such as colon cancer and trichothiodystrophy but what is the global effect of GTF2E on cellular processes is not widely characterized. In this regard, this study aimed to investigate and characterize the affect of GTF2E on transcription level of genes and identify the cellular processes and diseases associated with GTF2E. The HCT116 cell line was used in the study and transfected at 30 nM concentration with …

Antimetastatic Effect Of Nanodiamond-Conjugated Quercetin Against Colon Cancer: An In Vivo Study, Firli Rahmah Primula Dewi, Sephia Tiara Marviella, Sri Puji Astuti Wahyuningsih, A'Liyatur Rosyidah, Vuanghao Lim, Lionel Lian Aun In, Amy Yi Hsan Saik, Bimaji Ariyogo, Mee Lee Looi

Turkish Journal of Biology

Background/aim: Quercetin (Q) is a compound that can inhibit the growth of cancer cells in the colon; however, to do so, a high dose is needed, requiring a drug delivery system to target cancer endothelial cells directly. This study investigates the potency of nanodiamond-conjugated quercetin (NDQ) as an anticancer drug against colon cancer in Rattus norvegicus induced by N-methyl N-Nitrosourea (MNU). Materials and methods: This study is experimental-based and was designed using a six-group treatment method, namely normal control (KN: not treated by MNU, nanodiamond (ND), or Q); negative control (K–: treated by MNU); positive control (K+: treated by MNU …

Correction Of Griscelli Syndrome Type 2 Causing Mutations In The Rab27a Gene With Crispr/Cas9, Özgür Doğuş Erol, Şi̇mal Şenocak, Burcu Özçi̇men, Gülen Güney Esken, Hasan Basri̇ Kiliç, Yusuf Çeti̇n Kocaefe, Niek Peter Van Til, Fatima Aerts Kaya

Turkish Journal of Biology

Background/aimGriscelli Syndrome Type 2 (GS-2) is a rare, inherited immune deficiency caused by a mutation in the RAB27A gene. Current treatment consists of hematopoietic stem cell transplantation. Lack of suitable donors warrants the development of alternative treatment strategies, including gene therapy. The development of mutation-specific CRISPR/Cas9 gene editing technology has opened the way for custom designed gene correction of patient-derived stem cells. Here, we aimed to custom design CRISPR/Cas9 constructs and test their efficiency of homology directed repair (HDR) on the correction of exon 3 and exon 7 mutations in the RAB27A gene of GS-2 patient-derived mesenchymal stem cells (MSCs) …

Delivery Of Bikdd Proapoptotic Gene In Peptide-18 Targeted Poly(2-Oxazoline)-Dope Nanoliposomes For Breast Cancer Models, Zeynep Büşra Bolat, Ayça Ece Nezi̇r, Ongun Mehmet Saka, Itir Ebru Zemheri̇, Sevgi̇ Gülyüz, Umut Uğur Özköse, Özgür Yilmaz, Asuman Bozkir, Di̇lek Telci̇, Fi̇kretti̇n Şahi̇n

Turkish Journal of Biology

Breast cancer is the most common cancer type and the major cause of death in females worldwide. For effective breast cancer treatment, systems with promising delivery of anticancer agents is an important strategy. Peptide 18 (P18), a tumor homing peptide, shows high binding affinity towards breast cancer cells. Nanoliposomes are known to have enhanced accumulation ability in tumors with longer systemic circulation. Poly (2-ethyl-2-oxazoline) (PEtOx) polymers conjugated with DOPE were used to prepare PEtOx-DOPE nanoliposomes. BikDD is a mutant form of Bik gene, a member of the BH3-only proapoptotic genes, mimics the constitutively phosphorylated form of the gene. To the …

Disappearance Of Cdc14 From The Daughter Spindle Pole Body Requires Glc7-Bud14, İdi̇l Kirdök, Ayşe Koca Çaydaşi

Turkish Journal of Biology

Background/aim: The conserved phosphatase Cdc14 facilitates mitotic exit in budding yeast by counteracting mitotic Cyclin Dependent Kinase activity. Cdc14 is kept in the nucleolus until anaphase-onset, when its released transiently into the nucleoplasm. In late-anaphase, Cdc14 is fully released into the cytoplasm upon activation of the Mitotic Exit Network (MEN) to trigger mitotic exit. Cdc14 also localizes to yeast Spindle Pole Bodies (SPB) in anaphase and dephosphorylates key targets that resides at SPBs to license SPB duplication and prime the MEN. Protein Phosphatase 1 (Glc7) with its regulatory-subunit Bud14 is another phosphatase that plays a key role in spatiotemporal control …

Pterostilbene Suppresses Head And Neck Cancer Cell Proliferation Via Induction Of Apoptosis, Tali̇h Özdaş, Si̇bel Özdaş, İpek Canatar, Erdem Kaypak

Turkish Journal of Biology

Background/aim: Head and neck cancer (HNC) is one of the most prevalent causes of death worldwide, and the discovery of anticancer agents for its prevention is very important. Pterostilbene (PS) is a trans-stilbene reported to be beneficial in managing various cancers. The objective of the study was to evaluate the cytotoxic, anti-proliferative, pro-apoptotic and anti-migrative effect of PS on HEp-2, SCC-90, SCC-9, FaDu and Detroit-551 cell lines. Materials and Methods: MTT and live/dead assays were employed to assess cell-viability, while cell migration test was performed to evaluate wound healing capacity. The mRNA, protein and intracellular expression level of CASP-3, BAX …

Optical Imaging And Gene Transfection Potential Of Linear Polyethylenimine Coated Ag2s Near-Infrared Quantum Dots, Altay Savalan

Turkish Journal of Biology

Background/aimApplication of biocompatible heavy metal free and cationic Ag2S NIR QDs, which are highly luminesce in the medical range (700-900 nm), as a non-viral gene delivery system paves the way to overcome the auto-fluorescence and easily track the delivery of the genes in real time as it happens.Materials and methodsHere, the newly developed small and colloidally stable 2-mercaptopropionic acid (MPA)-capped Ag2S aqueous quantum dots electrostatically complexed with linear polyethyleneimine (LPEI) (Ag2S@2MPA/LPEI) was investigated for the first time both as a strong fluorescent probe and as a non-viral gene delivery vector for the highest tracking of the system and delivery of …

Anatolian Medicinal Plants As Potential Antiviral Agents: Bridging Traditional Knowledge And Modern Science In The Fight Against Covid-19 And Related Viral Infections, Engi̇n Ti̇lkat, Israt Jahan, Ayşe Hoşer, Alevcan Kaplan, Oğuzhan Özdemi̇r, Ahmet Onay

Turkish Journal of Biology

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the cause of the worldwide coronavirus illness 2019 (COVID-19), often known as the coronavirus pandemic. Since December 2020 however, COVID-19 vaccines have been extensively administered in numerous countries. In addition to new antiviral medications, the treatment regimen also encompasses symptom management. In spite of sustained research efforts, the outbreak remains uncontrolled, with affected patients still lacking proper treatment. Consequently, this review serves as a valuable asset for researchers and practitioners aiming to delve into the yet unexplored potential of Anatolian flora in the fight against COVID-19 and other viral infections. Numerous …

Expressions Of The Satellite Repeat Hsat5 And Transposable Elements Are Implicated In Disease Progression And Survival In Glioma, Sila Naz Köse, Tutku Yaraş, Ahmet Bursali, Yavuz Oktay, Ci̇hangi̇r Yandim, Gökhan Karakülah

Turkish Journal of Biology

The glioma genome encompasses a complex array of dysregulatory events, presenting a formidable challenge in managing this devastating disease. Despite the widespread distribution of repeat and transposable elements across the human genome, their involvement in glioma's molecular pathology and patient survival remains largely unexplored. In this study, we analysed the expression levels of satellite repeats and transposons along with genes in low-grade glioma (LGG) and high-grade glioma (HGG). Endogenous transposable elements LTR5 and HERV_a-int exhibited higher expression in HGG patients, along with immune response-related genes. Altogether, 16 transposable elements were associated with slower progression of disease in LGG patients. Conversely, …

Exploring The Impact Of Diabetes On Aging: Insights From Tert And Col1a1 Methylation, Jessica Nathania Liamry, Farizky Martriano Humardani, Giovani Chandra, Lisa Thalia Mulyanata, Tjie Kok, Fenny Irawati, Hikmawan Wahyu Sulistomo, Christoph Reichetzeder, Sulistyo Emantoko Dwi Putra

Turkish Journal of Biology

Background/aim: Aging, a multifaceted biological process, leading to diminished physical performance, especially in older adults with diabetes, where a mismatch between biological and chronological age is noticeable. Numerous studies have demonstrated that diabetes accelerates aging at both cellular and organ levels. Notable aging markers are TERT, related to telomere length, and COL1A1, a key component of skin collagen. Additionally, age-related methylation increases, as revealed through methylation analysis, illuminate aspects of aging. However, the detailed interplay between aging and diabetes, particularly regarding methylation, remains underexplored and warrants further study to elucidate their biological links.Materials and methods: In this research, we elucidate …

Bud14 Function Is Crucial For Spindle Pole Body Size Maintenance, Sevi̇lay Müni̇re Gi̇rgi̇n, Ayşe Koca Çaydaşi

Turkish Journal of Biology

Background/aimSpindle Pole Bodies (SPB), functional equivalent of centrosomes in yeast, duplicate through generation of a new SPB next to the old one. Yet, SPBs are dynamic structures that can grow and exchange, and mechanisms that regulate SPB size remain largely unknown. This study aims to elucidate the role of Bud14 in SPB size maintenance in Saccharomyces cerevisiae.Materials and methodsWe employed quantitative fluorescence microscopy to assess the relative and absolute amounts of SPB structural proteins at SPBs of wildtype cells and in cells lacking BUD14 (bud14∆). Quantifications were performed using asynchronous cell cultures as well as cultures synchronously progressing through the …

Lungbank: A Novel Biorepository Strategy Tailored For Comprehensive Multi-Omics Analysis And P-Medicine Applications In Lung Cancer, Di̇lek Demi̇rcan Çeker, Volkan Baysungur, Serdar Evman, İlker Kolbaş, Abdurrahi̇m Gördebi̇l, Si̇nem M. Nalbantoğlu, Yusuf Tambağ, Ömer Kaçar, Ahmet Mi̇di̇, Hati̇ce Aslanoğlu, Nülüfer Kara, Ni̇lgün Algan, Ayberk Boyacioğlu, Betül Karademi̇r Yilmaz, Ali̇ Şahi̇n, Hi̇vda Ülbeği̇ Polat, Abi̇di̇n Şehi̇toğullari, Ali̇ Osman Çibikdi̇ken, Mücahi̇t Büyükyilmaz, İbrahi̇m Berkan Aydi̇lek, Abdulkeri̇m Eneş, Sevde Küçüker, Fati̇h Karakaya, İhsan Boyaci, Mahmut Gümüş, Onur Şenol, Merve Öztuğ, Evren Saban, Ömer Soysal, Nur Büyükpinarbaşili, Akif Turna, Mehmet Zeki Günlüoğlu, Aslı Çakir, Şaban Teki̇n, Uygar Tazebay, Abdullah Karadağ

Turkish Journal of Biology

Background/aim: LUNGBANK was established as a pioneering biorepository dedicated to lung cancer. Designed for streamlined biospecimens handling, it employs high-volume technologies to secure high-quality samples. Fortified with robust data management, LUNGBANK efficiently integrates diverse datasets. This strategic infrastructure positions LUNGBANK as more than a biospecimens repository; it operates as a sophisticated platform for advanced lung cancer research, amplifying the potential for meaningful discoveries.Materials and methods: LUNGBANK is intricately designed to streamline the collection, transfer, storage, and processing of biospecimens, employing high-volume technologies to ensure the acquisition of high-quality biological specimens. The system is fortified with robust data management protocols, facilitating …

O-Demethyl Galantamine Alters Protein Expression In Cerebellum Of 5xfad Mice, İrem Kiriş, Merve Karayel Başar, Büşra Gurel, Tomasz Mroczek, Ahmet Tarik Baykal

Turkish Journal of Biology

Background/aim: Alzheimer’s disease (AD), one of the most common health issues, is characterized by memory loss, severe behavioral disorders, and eventually death. Despite many studies, there are still no drugs that can treat AD or stop it from progressing. Previous in vitro tests showed that O-demethyl galantamine (ODG) might have a therapeutic potential owing to its 10 times higher acetylcholinesterase inhibitory activity than galantamine (GAL). Materials and methods: We aimed to assess effect of ODG on a molecular level 12-month-old 5xFAD Alzheimer’s mouse model. To this end, following the administrations of ODG and GAL, used as a positive control, protein …

Petox-Dope Nanoliposomes Functionalized With Peptide 563 In Targeted Bikdda Delivery To Prostate Cancer, Ayca Ece Nezi̇r, Zeynep Büşra Bolat, Ongun Mehmet Saka, Itir Ebru Zemheri̇, Sevgi̇ Gülyüz, Umut Uğur Özköse, Özgür Yilmaz, Asuman Bozkir, Fi̇kretti̇n Şahi̇n, Di̇lek Telci̇

Turkish Journal of Biology

BackgroundNanocarrier-based systems have cultivated significant improvements in prostate cancer therapy. However, the efforts are still limited in clinical applicability, and more research is required for the development of effective strategies. Here, we describe a novel nanoliposomal system for targeted apoptotic gene delivery to prostate cancer.MethodsPoly (2-ethyl-2-oxazoline) (PEtOx) dioleoyl phosphatidylethanolamine (DOPE) nanoliposomes were conjugated with the prostate-specific membrane antigen (PSMA)-targeting peptide GRFLTGGTGRLLRIS (P563) and loaded with BikDDA, a mutant form of the proapoptotik Bik. We selected 22Rv1 cells with moderate upregulation of PSMA to test the in vitro uptake, cell death, and in vivo anti-cancer activity of our formulation, P563-PEtOx-DOPE-BikDDA.ResultsBikDDA was …

The Apoptotic Effect Of Garlic (Allium Sativum) Derived Sevs On Different Types Of Cancer Cell Lines In Vitro, Naz Ünsal, Polen Koçak Deni̇zci̇, Hazal Yilmaz, Fi̇kretti̇n Şahi̇n, Merve Yildirim Canpolat

Turkish Journal of Biology

Small extracellular vesicles (SEVs) are known to have an impact on the physiological circumstances of the target cells, are a critical component of cell-to-cell communication and have been implicated in a variety of diseases. Although it has been proposed that edible plant-derived nanoparticles have effect on communication with mammalian cells, the influence of these nanoparticles on cancer cell development has yet to be explored. In order to characterize small extracellular vesicles obtained from garlic, specific SEV surface markers and antibodies and size detections were performed by using scanning electron microscopy and nanoparticle tracking analysis. Human hepatoma, Hep3B, human neuroblastoma SH-SY5Y, …

Proteomic Investigation Of Acute And Chronic Hypoxia /Reoxygenation Responsive Proteins And Pathways In H9c2 Cardiomyoblasts, Merve Öztuğ, Evren Kilinç, Zeynep A. Öztuğ Durer, Emel Baloğlu

Turkish Journal of Biology

Background/Aim: Ischemic heart diseases continue to be a significant global cardiovascular problem in today's world. Myocardial reperfusion (R) is provided with an effective and rapid treatment; however, it can lead to fatal results as well as ischemia (I). This study aims to use proteomic analysis to assess proteins and pathways in H9C2 cardiomyoblast cells exposed to hypoxic conditions, followed by reoxygenation, representing I /R injury for both short and long terms, reflecting acute and chronic hypoxia, respectively. Utilizing advanced techniques, our goal is to identify and characterize key proteins undergoing alterations during these critical phases.Materials and methods: H9C2 cardiomyoblasts, commonly …

Non-Small Cell Lung Cancer Treatment: Current Status Of Drug Repurposing And Nanoparticle-Based Drug Delivery Systems, Tugba Gul Inci, Serap Acar, Di̇lek Balik

Turkish Journal of Biology

Drug repurposing is the strategy of drug utilization for a treatment option other than the intended indications and has increased over the past decades, especially within cancer nanomedicine. Cancer nanomedicine has been facilitated through nanoparticle-based (NP-based) delivery systems which can combat NSCLC via recent advances in nanotechnology and apply its benefits to existing drugs. An effective therapeutic solution could be gained via repurposing and accelerated via NP-based drug delivery systems. This review aims to present an overview of NSCLC treatments with a special focus on drug repurposing with details of clinical study advances and NP-based drug delivery systems for NSCLC. …

Nampt, Il-6, And Vaspin Gene Expressions And Serum Protein Levels In Type 2 Diabetes Mellitus And Related Complication, Süheyla Pınar Çelik, Damla Nur Parıltı, Leyla Açık, Mehmet Muhittin Yalçın, İlhan Yetkin, Eldeniz Yunusov

Turkish Journal of Biology

Type 2 diabetes (T2DM) is the most common type of diabetes and occurs due to insufficient insulin secretion or inability to use existing insulin and the effects of environmental factors. Although there are many studies on the pathophysiology of T2DM, the mechanisms playing a role in the pathogenesis of insulin resistance and pancreatic beta-cell dysfunction have not been fully elucidated. Some adipokines secreted from adipose tissue, which are the primary regulators of insulin resistance, affect immune and inflammatory functions. Altered adipokine profiles have been observed in obesity and T2DM, leading to severe metabolic risks and changes in insulin sensitivity. This …

A Novel Algorithm For The Virtual Screening Of Extensive Small Molecule Libraries Against Ercc1/Xpf Protein-Protein Interaction For The Identification Of Therapeutic Resistance-Bypassing Small Anticancer Molecules, Salma Ghazy, Lalehan Oktay, Serdar Durdagi

Turkish Journal of Biology

Background and aim:Cancer cell’s innate chemotherapeutic resistance continues to be an obstacle in molecular oncology. This theory is firmly tied to the cancer cells’ integral DNA repair mechanisms continuously neutralizing the effects of chemotherapy. Amidst these mechanisms, the nuclear excision repair pathway is crucial in renovating DNA lesions prompted by agents like Cisplatin. The ERCC1/XPF complex stands center-stage as a structure specific endonuclease in this repair pathway. Targeting the ERCC1/XPF dimerization brings forth a strategy to augment chemotherapy by eschewing the resistance mechanism integral to cancer cells. This study tracks and identifies small anticancer molecules, with ERCC1/XPF inhibiting potential, within …

Circ-Mettl15 Stimulates The Aggressive Behaviors Of Papillary Thyroid Cancer Cells By Coordinating The Mir-200c-3p/Xiap Axis, Yuhao Huang, Xinyu Zeng, Yanling Cai, Yan Yang, Yujie Zhang, Yiqi Ma, Suping Li

Turkish Journal of Biology

Background and objective: Thyroid cancer is the most common malignant tumor in the endocrine system. Papillary thyroid carcinoma (PTC) accounts for the vast majority of cases in this cancer. Recently, the vital role of circular RNA (circRNA) has been acknowledged in various cancers. However, to date, few studies have examined the roles of circRNAs in PTC. Therefore, this research aimed at exploring the function and mechanism of circ-methyltransferase-like 15 (METTL15) in PTC cells.Methods: First, quantitative measurements of circ-METTL15, miR-200c-3p, and X-linked inhibitor of apoptosis protein (XIAP) in PTC cells were completed with reverse transcription-quantitative polymerase chain reaction or Western blot …

Mir-770-5p-Induced Cellular Switch To Sensitize Trastuzumab Resistant Breast Cancer Cells Targeting Her2/Egfr/Igf1r Bidirectional Crosstalk, Senem Noyan, Bala Gür Dedeoğlu

Turkish Journal of Biology

Background/aim: Studies highlighted the bidirectional crosstalk between the HER family members in breast cancer as resistance mechanism to anti-HER agents. Cross-signaling between HER2/EGFR and ER/IGF1R could play role in the development of resistance to therapeutics hence stimulating cell growth. To overcome this resistance, combined therapies targeting both pathways simultaneously have been proposed as an effective strategy. The involvement of miRNAs in resistance of targeted therapies like trastuzumab was demonstrated in recent studies. Hence the regulation of miRNAs in resistance state could reverse the cell behaviour to drugs. Previously we found that overexpression of miR-770-5p downregulated AKT and ERK expression through …