Biology Commons^™

Different Hypoxic Response Of Human Cavii And Caenorhabditis Elegans Homologous Genes Cah-3, Cah-4, Cah-5, Sümeyye Aydoğan Türkoğlu, Aysu Bozkurt, Fatma Poyrazli, Derya Okuyan

Turkish Journal of Biology

Background/aim: A number of carbonic anhydrase (CA) family proteins have been implicated in cancer. They contribute to the hypoxic microenvironment. CAVII is often downregulated in colorectal carcinoma and it has been associated with increased tumor size, node metastasis, and adverse clinical outcomes. In this study, we aimed to investigate the effect of hypoxia on CAVII protein in human colon cancer and prostate cancer cells. In addition, the regulation of CAH genes in Caenorhabditis elegans was examined. These are homologous to CAVII in humans.

Materials and methods: CAVII expression was analyzed in different cell lines such as human colon cancer (SW480 …

Mir-9-5p Alleviates The Development Of Abdominal Aortic Aneurysm By Regulating The Differentiation Of Cd4+Il-10+T Cells Via Targeting The Crosstalk Between Nrf2 And Nf-Κb Signaling Pathways, Hongfu Liu, Jinyi Zhang, Lubin Li, Benxiang Yu, Chunlei Zhang, Wenqiang Niu, Yawen Cheng, Hengyang Dong, Yukun Zhang, Xinlin Luo, Yanlian Xiong, Yueming Wang

Turkish Journal of Biology

Background: Abdominal aortic aneurysm (AAA), a gradual segmental dilatation of the abdominal aorta, is associated with a high mortality rate. The pathophysiological molecular mechanisms underlying AAA remain unclear. In recent years, changes in miRNA levels have been reported to be involved in the development and treatment of AAA. This study aimed to investigate the potential targets and underlying mechanisms of miR-9-5p in attenuating AAA progression by modulating the inflammatory response.

Materials and methods: Biochemical kits were used to measure the levels of inflammatory factors, antioxidant enzyme activity, and serum oxidative stress in normal and AAA model mice. miR-9-5p overexpression was …

Lactic Acid Bacteria Alleviate Neuronal Excitotoxicity And Extend Caenorhabditis Elegans Lifespan, Bao Le, Thi Nhat Hong Nguyen, Seung-Hwan Yang

Turkish Journal of Biology

Aim: Neuronal cell death plays a critical role in the development of neurological disorders associated with aging. This study aimed to evaluate the beneficial effects of heat-killed lactic acid bacteria (hkLAB) on neuroblastoma cells and Caenorhabditis elegans.

Materials and methods: We pretreated heat-killed Lactobacillus fermentum CNU384 (hkCNU384), L. brevis CNU386 (hkCNU386), L. helveticus CNU395 (hkCNU395), and L. paracasei CNU396 (hkCNU396) at a concentration of 10⁹ CFU/mL to investigate their neuroprotective effects on glutamate-induced SH-SY5Y cells and their impact on the lifespan of 6-hydroxydopamine (6-OHDA)-induced C. elegans.

Results: Our findings indicate that hkCNU395 and hkCNU396 protected against glutamate-induced cell death …

Insights Into Therapeutic Discovery Through The Kelch Domain Structure Of Keap1 At Ambient Temperature, Merve Yilmaz, Belgi̇n Sever, Yi̇ği̇t Kutlu, Mehmet Gül, Ceren Okuducu, Serra Tavli, Masami Otsuka, Mikako Fujita, Turkan Hali̇loğlu, Hali̇li̇brahi̇m Çi̇ftçi̇, Hasan Demi̇rci̇

Turkish Journal of Biology

Background/aim: The Kelch-like-ECH associated protein 1 (Keap1) is an integral component of the E3-ubiquitin ligase complex, which binds to Nuclear factor erythroid 2-related factor 2 (Nrf2) and facilitates its degradation by the 26S proteasome. The Kelch domain of Keap1, composed of six repeated structural motifs, plays a key role in this interaction. This study aims to investigate the dimeric structure of the Keap1 Kelch domain at ambient temperature and to examine its implications for conformational dynamics, particularly in relation to the DMF and Nrf2 binding sites.Materials and methods: The dimeric crystal structure of the Keap1 Kelch domain was determined at …

The Effects Of Hsa-Mir-26a-5p On Cell Proliferation, Migration, And Pi3k Inhibitor Sensitivity In Metformin-Resistant Triple Negative Breast Cancer Cells, Şahi̇ka Cingir Köker, Senem Noyan, Banu Yalçin, İrem Doğan Turaçli

Turkish Journal of Biology

Background/aim: Metformin is commonly used to manage type 2 diabetes (T2D) and is being investigated for its potential antiproliferative effects in cancer, particularly in patients with both T2D and malignancies. Drug resistance can develop with any therapeutic agent, and metformin is no exception. As we showed in our previous study, metformin-resistant MDA-MB-468 (MET-R) cells exhibited an EMT-like phenotype. Many transcription factors, as well as miRNAs, can contribute to this altered phenotype. Our current study identifies the contribution of hsa-miR-26a-5p expression to the previously observed phenotype. Materials and methods: By utilizing bioinformatic tools, we identified hsa-miR-26a-5p, whose expression was significantly altered …

Butyrylcholinesterase (Bche) Downregulation In Taxane Resistance: Implications For Prostate Cancer, Buse Cevatemre, Arda Işiklar, İpek Bulut, Ezgi̇ Karyemez, Hamzah Syed, Ceyda Açilan

Turkish Journal of Biology

Background/aim: Despite advancements in chemotherapeutic strategies, drug resistance remains a major barrier to effective cancer treatment. While primary prostate cancers (PC) can be treated with surgery and radiotherapy, treatment options for recurrent PC are limited. Upon progression to the castration-resistant (CR) phase, therapies rely on taxanes (chemotherapeutic drugs) like docetaxel (Dtx) and cabazitaxel (Cbz); however, resistance to taxanes is common in CRPC, highlighting the importance of identifying underlying molecular mechanisms or targets in resistant cells.Materials and methods: In transcriptome (RNA sequencing) analyses comparing taxane-resistant PC cells (resistant to both Dtx and Cbz) with parental (nonresistant, sensitive) cells, butyrylcholinesterase (BChE) was …

Proteomic Profiling Of Escherichia Coli Cytoplasmic Proteins Under Sublethal Boric Acid Stress, Beki̇r Çöl, Begüm Hazar Çi̇ftçi̇, Merve Sezer Kürkçü, Esra Di̇bek

Turkish Journal of Biology

Background/aim: Boron is an essential micronutrient for plants and certain bacteria, where it plays critical roles in cellular processes at low concentrations. However, elevated levels of boron-containing compounds, such as boric acid, exhibit antimicrobial toxicity. Although the physiological effects of boric acid on bacteria have been partially characterized, its proteome-wide impacts remain poorly elucidated. This study employs a 2D-PAGE-based proteomic approach to investigate how sublethal boric acid stress alters the cytoplasmic proteome of Escherichia coli BW25113. Materials and methods: E. coli BW25113 cultures were grown to mid-log phase in tryptic soy broth (TSB) and exposed to 70 mM boric acid …

Screening Of Ccdc43 Molecular Partners By Bioid2-Based Proximity Labeling, Merve Tuzlakoğlu Öztürk

Turkish Journal of Biology

Background/aim: CCDC43 (coiled-coil domain containing protein 43) is a eukaryotic protein that contains alpha-helical domains in its structure, consistent with the family to which it belongs. It is predominantly located in the cytosol of the cell. CCDC43 protein has been shown to play a role in cell proliferation, invasion, metastasis, and epithelial–mesenchymal transition in gastric and colorectal cancer types. The fundamental function and cellular interaction network of this protein, which is known to have varying expression levels in various cancer types, has not yet been fully elucidated. Here, we conducted a proximity-dependent biotin identification (BioID2) screening approach for CCDC43 to …

Fda-Approved Drugs As Potential Covalent Inhibitors Of Key Sars-Cov-2 Proteins: An In Silico Approach, Murat Seri̇lmez, Anwar Abuelrub, İsmai̇l Erol, Serdar Durdaği

Turkish Journal of Biology

Background/aim: The COVID-19 pandemic caused by SARS-CoV-2 necessitated rapid development of effective therapeutics, prompting this study to identify potential inhibitors targeting key viral and host proteins: RNA-dependent RNA polymerase (RdRp), main protease (Mpro), transmembrane serine protease 2 (TMPRSS2), and angiotensin-converting enzyme 2 (ACE2). Methods: We used covalent docking and molecular dynamics (MD) simulations to screen FDA-approved compounds against these targets using diverse covalent reaction mechanisms. Top-ranking compounds underwent further evaluation through MD simulations to assess binding stability and conformational dynamics. Results: Several promising drug repurposing candidates were identified: bremelanotide, lanreotide, histrelin, and leuprolide as potential RdRp inhibitors; azlocillin, cefiderocol, and …

Antiinflammatory Effects Of Cucurbitacins And Sorafenib In Hepg2 Cells By Modulating The Iκb/Nf-Κb/Cox-2 Pathway Through Akt Signaling, Muhammed Mehdi̇ Üremi̇ş, Yusuf Türköz, Nuray Üremi̇ş

Turkish Journal of Biology

Aim: Cucurbitacins possess antitumor, antiproliferative, and antiinflammatory properties. This study aims to examine the antiinflammatory effects of CuD, CuI, and CuE on NF-κB, iNOS, COX-2, and Akt and compare their cytotoxic and antiinflammatory effects on HepG2 cells with sorafenib, the primary chemotherapeutic agent used in HCC treatment. Methods: Cytotoxic effects of cucurbitacins and sorafenib on HepG2 cells were evaluated using MTT and LDH assays, along with Annexin V, MMP, and comet assays. Levels of proteins related to the Akt/NF-κB pathway and COX-2, iNOS, and NO were also measured. Results: Our study showed that CuD, CuI, CuE, and sorafenib have antiproliferative …

Novel Mrsa-Targeting Phage Metb16: Genomic Features, Structural Insights, And Therapeutic Applications, Berna Erdoğdu, Senanur Dokuz, Görkem Güngör, Wei Lin, Yigang Tong, Tüli̇n Özbek

Turkish Journal of Biology

Background/aim: Recent reports have indicated that multidrug-resistant strains of S. aureus, including methicillin-resistant strains, may pose a significant threat to public health and global economic stability. Materials and methods: In this study, we present the isolation and comprehensive characterization of a novel phage, derived from clinically isolated MRSA strains. Results: MetB16 exhibited an incubation period of approximately 20 min, a lysis period of around 45 min, and a burst size of 127 Plaque Forming Units (PFU)/cell. The phage demonstrated remarkable biological stability across a pH spectrum of 4.0–9.0 and maintained integrity within a temperature range of 37 and –80 °C. …

Discovery And Characterization Of Selective Lipase-Inhibiting Polyheterocyclic Derivatives: A Combined In Silico And In Vitro Study, Madjda Benguechoua, Mebarka Imane Benguechoua, Khedidja Benarous, Houssem Boulebd, Ibtissem Kadi, Ari̇f Mermer, Yakup Şi̇ri̇n, Alaeddine Kaouka, Mohamed Yousfi

Turkish Journal of Biology

Background/aim: Obesity has become a global health crisis with an increasing prevalence, necessitating the search for effective therapies. Schiff base derivatives, known for their broad pharmacological activities, have gained attention as potential antiobesity agents. This study aimed to investigate the lipase inhibitory potential of novel Schiff base derivatives and assess their drug-like properties through in vitro assays and in silico methods. Materials and methods: The lipase inhibitory activity of synthesized Schiff base derivatives was evaluated using in vitro assays, with IC50 values determined for each compound. Additionally, in silico ADMET predictions (absorption, distribution, metabolism, excretion, and toxicity), molecular docking studies, …

Expression Of Concern: Characterization Of Tfiie-Regulated Genes By Transcriptomeanalysis, Serdar Baysal

Turkish Journal of Biology

Update: This Expression of Concern has been superseded by a Retraction Notice. The Expression of Concern remains part of the permanent publication record. Please see the Retraction Notice and the original article.

The Editors of Turkish Journal of Biology wish to issue an Expression of Concern regarding the article entitled: “Characterization of TFIIE-regulated genes by transcriptome analysis” by Serdar Baysal published on 20.12.2024. Following the publication of this article, concerns have been raised regarding• Potential violations of academic ethical standards in the published study• Potential intellectual property infringement, including plagiarism• An academic sanction reportedly imposed on the author by …

Apigenin Attenuates Ischemia–Reperfusion-Induced Pulmonary Ferroptosis And Fibrosis By Activating The Nrf2/Ho-1/Gpx4 Axis In Mice, Liang Zhang, Haojie Li, Shichen Xu, Hao Wen, Chaoxiao Yu

Turkish Journal of Biology

Background/aim: Acute lung injury (ALI) is a major cause of morbidity and mortality after lung ischemia-reperfusion injury (LIRI). In recent years, pulmonary ferroptosis and its associated fibrosis have been recognized as important causes of LIRI. The purpose of this study is to investigate apigenin (APG) as a potential therapeutic target for treating LIRI-induced pulmonary ferroptosis and fibrosis.

Materials and methods: A rat model of LIRI was established and the rats were randomly divided into three groups, a sham group, a LIRI group, and an APG group. The pathological changes of the lung tissue were evaluated using hematoxylin-eosin staining and …

Progression Of Electrophysiological Impairments In Diabetic Cardiomyopathy And Intervention Using Enicostemma Axillare, Victor Arokia Doss, Dharaniyambigai Kuberapandian

Turkish Journal of Biology

Earlier studies widely reported advanced ventricular impairments only at the later stages of diabetic cardiomyopathy (DCM). The present study aimed to understand the duration-based early to late stages of cardiac electrophysiological impairments using electrocardiography (ECG) and intervention using Enicostema axillare subsp. littorale. The experimental rats were streptozotocin (SZ)-induced diabetic rats that were administered E. axillare formulations as follows: (1) normal; (2) SZ - 40 mg/kg, single, i.p.); (3) SZ + insulin (2 U/day) + losartan (10 mg/kg); (4) SZ + insulin + losartan + E. axillare decoction (2 mL/day); (5) SZ + E. axillare decoction; (6) SZ + E. axillare …

Knockdown Of Plexin C1 Induces Epithelial-To-Mesenchymal Transition And Confers Resistance To Multikinase Inhibitors In Hepatocellular Carcinoma Cells, Gamze Güngör Topcu, Arzu Aysan, Şevval Di̇k, Mai̇de Şeker, Meli̇ke Bi̇nnur Bahçekapili, Sude Topkaraoğlu, Di̇lek Yavuzer, Tamer Yağci

Turkish Journal of Biology

Background/aim: HCC is a common and lethal malignancy and multi-kinase inhibitors (MKIs) are among the therapeutic options for unresectable cases. However, response rates to MKIs remained variable, necessitating the identification of predictive biomarkers. Plexin C1 (PLXNC1), a receptor involved in cell signaling, has emerged as a potential candidate to regulate tumor responses. This study aims to evaluate the impact of PLXNC1 expression on the sensitivity of HCC cells to MKI therapy.

Materials and methods: shRNA-mediated PLXNC1 knock-down and control clones of HCC cell lines PLC/PRF/5 and Hep3B were generated, and downregulation of PLXNC1 was confirmed using Western blotting. …

A Tetracycline-Inducible Split Turboid System For Specific Biotinylation And Identification Of Nuclear Proteins From Hek293t Cells, Mehmet Sarihan, Fatma Zehra Özen, Murat Kasap, Gürler Akpinar

Turkish Journal of Biology

Background/aim: To overcome the limitations of conventional organelle isolation methods including low purity, low yield, sample degradation, scalability and the need for multiple centrifugation steps, an improved nuclear protein enrichment approach was developed using the modified Split TurboID biotin ligase enzyme.

Materials and methods: A construct was created in which the N-terminal domain of TurboID, fused to the FK506-binding protein (FKBP) was targeted to the nucleus. This construct was incorporated into a tetracycline-inducible gene expression vector. Similarly, the C-terminal domain of TurboID was fused to the rapamycin-binding domain of mTOR (FRB) and directed to the nucleus. This construct …

Prooxidant Effect Of Uric Acid On Human Leukocytic Dna: An In Vitro And Ex Vivo Study, Yim Tong Savio Szeto, Vincy Sze Wing Li, Yuen Lam Pon

Turkish Journal of Biology

Background/aim: Uric acid is a major contributor to the total antioxidant capacity of human plasma. However, this endogenous substance’s antioxidant and prooxidant properties have not yet been reported.

Conclusion: In this study, the comet assay was employed in vitro to determine the effect of uric acid on DNA damage in human lymphocytes and leukocytic DNA damage in hyperuricemia patients with and without renal failure.

Results: DNA damage in lymphocytes occurred at uric acid concentrations of ≥600 μM. Adding catalase to the uric acid solution diminished the damaging effect, indicating that hydrogen peroxide mediated the prooxidant activity. Moreover, …

Intrinsic Apoptotic Effect Of Anatolian Honeybee (Apis Mellifera Anatoliaca) Venom Promoted With Mesoporous Silica Nanocarriers, Batuhan Orman, Ayli̇n Koç, Di̇dem Şen Karaman, Ayşe Nalbantsoy

Turkish Journal of Biology

Background/aim: The use of bee products or treatment with bees, as a complement to conventional medicine is attracting considerable attention in cancer research. Although discoveries related to the potential anticancer effects of bee venom are increasing, the unstable nature of venom biomolecules remains a limiting factor for their usage. In this study, we employed mesoporous silica nanocarriers (MSNs) to provide precise dosing and prevent carriers from biomolecule degradation thanks to the outstanding loading capacity provided by the pores, excellent chemical and biological robustness, and ability to improve bioavailability.

Materials and methods: MSNs were synthesized and physicochemical characterizations were …

N-Cadherin Mimetic Hydrogels Drive Superior Regenerative And Paracrine Responses In 3d Cultures Of Adipose-Derived Mesenchymal Stem Cells, Demet Kaçaroğlu, Alper Murat Ulaşli, Aybüke Di̇denur Sak, Seher Yaylaci

Turkish Journal of Biology

Background: Cadherin-based biomaterials play a pivotal role in influencing the fate of mesenchymal stem cells (MSC). Enhancing the adhesion of adipose tissue-derived MSCs has been shown to augment their paracrine effects while N-cadherin biomaterials have been suggested to regulate the paracrine effects of MSCs via specific growth factors although the precise mechanisms underlying this regulation remain insufficiently understood. This study aims to compare the effects of a 3D N-cadherin mimetic environment on cell viability, apoptosis, extracellular matrix regulation, and growth factor expression with those observed in traditional 2D and 3D spheroid cultures. Additionally, the study seeks to evaluate the …

Resveratrol Modulates Triosephosphate Isomerase And Mineralization In Osteosarcoma Cells: Potential Target For Novel Therapeutic Strategies, Gonca Tuna, Si̇bel Çinar Asa, Eli̇f Ertürk, Yaren Yildiz, Ferda Ari

Turkish Journal of Biology

Background/aim: Osteosarcoma, a primary malignant bone tumor, is challenging to treat due to its aggressive nature and limited therapies. Resveratrol (RES), a natural polyphenol, has potential anticancer properties. Hence, we investigated RES’s impact on osteosarcoma cells, focusing on triosephosphate isomerase (TPI) and related mechanisms.

Materials and methods: RES was applied to osteosarcoma (SaOS-2) and healthy fetal osteoblast (hFOB 1.19) cells for 48 h, and cell viability was measured by SRB assay. The mode of cell death was examined using Hoechst 33342/annexin V/propidium iodide. TPI and methylglyoxal (MG) enzyme levels were determined by ELISA. The effect of RES on …

The Therapeutic Potential Of Salvia Aegyptiaca Extracts In Ethanol-Induced Gastric Ulcer:Insights Into Macroscopic, Histopathological, And Biochemical Mechanisms, Walid Mamache, Smain Amira, Fatima Benchikh, Hassiba Benabdallah, Amor Bencheikh, Hind Amira, Roumaissa Ounis, Mohammed Abdallah Torki, Chahrazed Kaoudoune

Turkish Journal of Biology

Background/aim: This study explores the antiulcer activity of different doses of Salvia aegyptiaca (SAE) methanol (ME) and decoctedextracts (DE) on ethanol-induced gastric ulcers in rats.Materials and methods: Female Wistar rats weighing 180–200 g were divided into five groups: control, omeprazole (positive control),and extract-treated (100, 200, and 400 mg/kg). Ulcers were induced with absolute ethanol 30 min after treatment with the extracts.The experiment was followed by macroscopic and histopathological examination. In vitro tests were also conducted to assess lipidperoxidation, catalase activity, mucus content, glutathione, and protein levels.Results: The study found that 100% ethanol caused significant damage, including colour and mucus loss, …

Antidiabetic Potential Of Polysaccharides From Algerian Saharan Zygophyllum Geslini Instreptozotocin-Induced Diabetic Rats, Houria Medjdoub, Waffa Bouali, Arezki Azzi, Nacera Belkacem, Nabila Benariba, Nawel Meliani

Turkish Journal of Biology

Background/aim: Zygophyllum geslini, an endemic Algerian species, has numerous properties, especially as an antidiabeticdrug. In Algeria, this herb serves as condiment in Saharan dishes and as animal feed (Sheep, Goat and Camel). However, fewscientific studies have reported on the medicinal properties of this Saharan species. The aims of the present work were to study 1) thechemical aspects of polysaccharides extracted from this plant, 2) their inhibitory effect on pancreatic α-amylase in vitro, and 3) theirantihyperglycemic effect in streptozotocin-induced diabetic rats in vivo.Materials and methods: First, polysaccharides were extracted from Z. geslini aerial part (ZGAP) in hot water and precipitated withethanol …

Inhibitory Effects Of Carvacrol On Glucansucrase From Streptococcus Mutans And Salivaryα-Amylase: In Silico And In Vitro Studies, Samet Kocabay, M. Abdullah Alagöz, Bi̇rnur Akkaya

Turkish Journal of Biology

Background/aim: Streptococcus mutans produces glucansucrase, an enzyme that converts sucrose into lactic acid, which lowers the pH in the oral environment and leads to tooth enamel demineralization, a key factor in dental caries. Additionally, glucansucrase facilitates the formation of extracellular polysaccharides, which promote bacterial adhesion to tooth surfaces. This study investigates the inhibitory effects of carvacrol, a natural compound, on glucansucrase activity both in vitro and in silico. Materials and methods: Glucansucrase enzyme was purified from S. mutans. The inhibitory effects of carvacrol against glucansucrase enzyme were investigated both in vitro and in silico. Results: In the presence of 50 …

Investigating The Composition Of Fatty Acid, Tocopherol, And Sterol Contents Andantioxidant Capacity Of Oils From The Seeds Of Abelmoschus Esculentus L. Growing Inalgeria, Samira Nia, Madjda Benguechoua, Toufik Hadj Mahammed, Khatir Reggagui, Mohamed Yousfi

Turkish Journal of Biology

Background/aim: Vegetable oils are considered a vital source of energy when glucose cannot be utilized. Therefore, research on newsources of oil that demonstrate nutritional value is necessary. Okra (Abelmoschus esculentus), a member of the Malvaceae family, isa remarkable botanical specimen due to its various uses; its roots, stem, leaves, fruits, and seeds are all valued for their nutritional,therapeutic, and industrial value. This study aims to demonstrate okra’s fatty acid (FA) composition, tocopherol content, and sterolcontent and evaluate the antioxidant activity of the oils extracted from okra using three solvents.Materials and methods: The initial part of this study involved oil extraction …

Evaluating The Multifaceted Bioactivity Of Syzygium Aromaticum Essential Oil:The Central Role Of Eugenol, Noureddine Rahim, Chamseddine Derabli, Amina Bramki, Sara Mahdjoub, Sandrine Rup-Jacques, Ghozlane Barboucha, Stephani Hesse, Houssem Boulebd

Turkish Journal of Biology

Background/aim: Syzygium aromaticum L. is a versatile plant traditionally used to treat digestive and respiratory issues, improve oral health, and relieve pain, particularly in regions such as Southeast Asia, South Asia, and parts of the Middle East. Its essential oil (EO), predominantly composed of eugenol, is rich in bioactive compounds. This study aims to clarify the specific contribution of eugenol to the antioxidant, antibacterial, antifungal, and insecticidal activities of S. aromaticum EO by comparing their individual effects. Additionally, density functional theory (DFT) calculations were employed to examine the antioxidant mechanism of eugenol and its role in enhancing the overall activity …

Phytochemical Analysis And Antioxidant, Antimicrobial, And Cytotoxic Activities Ofxanthium Strumarium L. (Asteraceae), Ahmet Beyatli

Turkish Journal of Biology

Background/aim: Medicinal plants are considered an important source of novel antioxidant, antimicrobial, and anticancer agents. The main goal of this study was to define the beneficial properties of various extracts obtained from Xanthium strumarium. Materials and methods: Evaluations were conducted on the total phenolic and flavonoid contents of the extracts. High-performance liquid chromatography and diode-array detection (HPLC-DAD) was used to determine the phenolic profiles of the extracts. DPPH, ABTS, and FRAP assays were used to evaluate the free radical scavenging properties of the X. strumarium extracts. The broth dilution method was used for antimicrobial activity assessments, the MTT assay was …

Antilipase Activities Of Cultivated Peppermint And Rosemary Essential Oils:In Vitro And In Silico Studies, Khadidja Bengana, Talia Serseg, Khedidja Benarous, Ari̇f Mermer, Yakup Şi̇ri̇n, Alaeddine Kaouka

Turkish Journal of Biology

Background/aim: The growing interest in essential oils clearly indicates the power of nature and aligns with our increasing need to findtherapeutic solutions in the natural world. This study aimed to investigate the inhibitory effects of the essential oils of Mentha × piperitaand Salvia rosmarinus, harvested from the Laghouat region of Algeria, against Candida rugosa lipase (CRL) and pancreatic lipasethrough both in vitro and in silico studies.Materials and methods: Essential oils were extracted via hydrodistillation and analyzed using gas chromatography–mass spectrometryand spectrophotometry. Their antilipase activities were assessed using an inhibition assay, and molecular docking was performed withAutoDock Vina to explore interactions …

Phytochemical Profile And Nematicidal Potential Of Essential Oil From Algerian Wildoriganum Vulgare Subsp. Glandulosum Defs., Amina Mezerket, Juan Emilio Palomares Rius, Souad Bouasla, Henia Saib

Turkish Journal of Biology

Background/aim: The root gall nematode Meloidogyne incognita constitute the most damaging species that infects many crops inAlgeria. The intense use of harmful agricultural chemical products has incited research to develop alternative methods with natural andecological advantages like essential oils extracted from plants. The objective of this study was to evaluate the efficacy of Origanum vulgaresubsp. glandulosum Desf. (Lamiaceae) essential oil on the development of the root-knot nematode M. incognita in potted tomatoes.Materials and methods: In pot trials, we assess the activity of O. vulgare subsp. glandulosum essential oil at two concentrations of0.75 and 0.37 mg /L against M . incognita. …

Metal Complexes Of Sulfamethazine/Benzoin-Based Schiff Base Ligand: Synthesis,Characterization, Dft Calculations, And Antimicrobial Activities, Kerem Buran

Turkish Journal of Biology

Background/aim: The global rise in bacterial resistance poses a significant challenge, exacerbated by the overuse of antibacterial agents. Schiff base-metal complexes have gained attention for their potential antimicrobial properties, attributed to their unique three-dimensional structures and modes of action, such as cell wall inhibition and membrane disruption. The aim of this study was to synthesize and characterize a novel Schiff base (L) derived from sulfamethazine and benzoin, and to develop metal complexes of it with Cu2+, Co2+, Ni2+, and Cd2+. This research sought to evaluate the antibacterial and antifungal activities of the synthesized Schiff base and its metal complexes to …