Biology Commons^™

Isolation And Characterization Of Bacterial Symbionts From Crotalaria Spectabilis Grown On Trichloroethene Contaminated Soil, April Lyn Rocha

Masters Theses

"The discovery of several members of the Leguminosae family growing on a TCE contaminated site demonstrated that they have the capabilities to tolerate the TCE contamination. This led to an investigation of the plants' ability to metabolize TCE. Research by Dr. Lee Newman showed that nodules from Crotalaria spectabilis grown on the site had TCE metabolites present while nodules from other legumes at the site did not. The presence of TCE metabolites within the C. spectabilis nodules suggests that the bacterial symbiont may be responsible for TCE metabolism. This led us to investigate the symbiont of C. spectabilis and its …

Bat Habitat Use And Roost Tree Selection For Northern Long-Eared Myotis (Myotis Septentrionalis) In North-Central Ohio, Timothy J. Krynak

Masters Theses

Understanding roosting behavior and habitat use of bats is an important component when unraveling life histories and their ecology. Ohio remains under-represented in published information of bats compared to surrounding states. This large scale survey in 2002–2003 and Myotis septentrionalis radio telemetry study in 2005 is one of the few conducted within the state and is the first in Ohio’s North-Central region. It is also the first study conducted where net sites were chosen randomly and spatially distributed to adequately survey bat populations within Cuyahoga Valley National Park and Cleveland Metroparks. This survey documented seven species (n = 668), with …

The Seasonality Of Parasites In Illinois House Sparrows (Passer Domesticus): Effect Of Stress On Infection Parameters, Tiffany C.M. Gibson

Masters Theses

Parasitism often shows seasonal variation in their hosts and has been associated with host densities, host stress levels or transmission strategies (Moller et al 2003, Andersen and May 1982 ). This study was designed to evaluate the role of direct versus indirect life cycle transmission strategies, using Isospora and haemosporidians; and the impact of proposed ecological (winter) and physiological (breeding) stresses on parasitism levels. Coccidia have direct life cycles and infect gut tissues and are passed by fecal-oral transmission. Haemosporidians are blood parasites with an indirect life cycle that requires blood-feeding insects for transmission. If parasitemias are influenced by host …

Multiple Endpoints Of Endocrine Disruption In Gravid Watersnakes (Colubridae:Nerodia) As A Function Of Ingestion Of A Common Herbicide, Lorin Anne Neuman-Lee

Masters Theses

Ecotoxicological studies that focus on a single endpoint might not accurately represent the true ecological effects of a contaminant. Exposure to atrazine, a widelyused herbicide, disrupts endocrine function and sexual development in amphibians, but studies involving reptilian species are lacking. This study examines several effects of atrazine ingestion on gravid females and neonates exposed in utero of the Northern Watersnake (Nerodia sipedon). Twenty-five gravid N. sipedon were collected and kept in the lab for the entirety of their gestation period. Each snake received one of four doses of atrazine: control, 2, 20, or 200 ppb. Blood samples were drawn each …

A Spatially Explicit Model To Predict Radiocesium Body Burdens In White-Tailed Deer On The Department Of Energy's Savannah River Site, Christopher W. Bobryk

Masters Theses

A spatially explicit exposure model was developed to interpolate and predict radiocesium (137Cs) body burdens found in white-tailed deer (Odocoileus virginianus) on the U.S. Department of Energy's Savannah River Site (SRS) in west-central South Carolina. Since 1965 hunting has been allowed from permanent stands as a mechanism to manage the herd and all animals have been monitored in the field for gross beta/gamma activity levels providing a long-term spatially explicit dataset. Until now no study has attempted to model deer 137Cs at the hunt-stand level, but rather at the compartment level (1,000 - 5,200 ha). The models described here use …

The Spatial Distribution Of Japanese Beetles, Popillia Japonica, In Soybean Fields, Stacey A. Sara

Masters Theses

The Japanese beetle, Popilliajaponica Newman, is a serious pest of many agricultural and horticultural plants. Little is known about the distributions of Japanese beetles in agricultural fields which makes pest managyment more difficult. We studied the spatial distribution of Japanese be~tles in soybean fields. In particular, we examined how distance from an edge, edge direction, and edge type affected the distribution and abundance of beetles. Twenty-five soybean fields were transected parallel with the field edges at varying distances from the field edges and sampled for beetles. An edge effect for density was discovered; beetle numbers decreased significantly with increasing distance …

Prey Preference As A Function Of Feeding Experience And Prey Type In Neonate Gartersnakes (Colubridae: Thamnophis), Kathryn B. Hale

Masters Theses

Several species of potential prey have been introduced into the geographic range of the Two-striped Gartersnake, Thamnophis hammondii. Some of these prey species possess defenses that present novel challenges to the snake predator. Despite novel predator defense mechanisms (including secretions from skin glands), adult T. hammondii ingest several of these prey species without adverse effects. Although ~dult T hammondii are dietary generalists, almost nothing is known about prey preferences in newborn individuals that lack feeding experience. My study determined whether or not T. hammondii innately exhibits a preference among native and introduced prey types. Furthermore, I compared the degree of …

A Model Cost Analysis Comparing Solar, Wind And Geothermal Energies, Including Alternatives To Changing Energy Sources, Using Texas Households And Weather, Kimberly Speed

Masters Theses

The Emergency Economic Stabilization Act of2008 (P.L. 110-343) provides several incentives and tax credits for Americans interested in investing in alternative energy resources. Solar, wind, and. geothermal systems receive the most tax credits due to their low carbon output, but a determination must be made as to which resource is the most accessible and economically feasible. The State of Texas was used as a model for comparing the three renewable energy resources through analysis of weather variables, geological features, resource availability, amount of energy harvested in relation to equipment, and installation and maintenance costs.

All three energy systems have positive …

The Capacity Of Diatom Species To Survive Ingestion By The Algivorous Minnow, Pimephales Notatus, Paul Gregory Grubach

Masters Theses

The capacity of diatom species to survive gastrointestinal passage through the algivorous minnows Pimephales notatusand Campostoma anomalum was studied. From a site on the Grand River in northeastern Ohio, 27 minnows and 7 epilithic diatom samples were taken. In order to determine whether diatom taxa varied in digestibility, live/dead ratios of diatom cells taken from the minnows’ feces were compared with live/dead ratios of cells taken from immersed rocks. Diatoms that were live/undigested at the time of collection were differentiated from dead/digested cells under light microscopy by noting the presence of chloroplasts and/or lipid droplets. Seventy-seven percent of the …

The Role Of Skeletal Development In Body Size Evolution Of Two North American Frogs, Sarah Beth Havens

Masters Theses

"In order to better understand the evolution of miniaturization in Acris blanchardi, a North American Hylid with a unique life history and of ecological interest in the United States. The development and ossification sequences of 48 larvae, 5 juveniles and 5 adult A. blanchardi were examined. The adult was described by Maglia et al. (2007) to be a miniature and to display morphological novelties. In addition, 44 larvae, 2 juvenile and 5 adults of Pseudacris crucifer a closely related frog that has been suggested to be a miniature, were examined. The cranial and postcranial adult elements of this species, as …

Does Evolutionary Exposure Mediate Allelopathic Effects?, Nikki Leigh Pisula

Masters Theses

Allelopathy most commonly refers to chemically mediated plant interference, though the effects may range from beneficial to inhibitory. Allelopathy has been suggested as a potential mechanism for invasion success because invasive species may produce allelochemicals that are evolutionarily novel to recipient communities. To test for evolutionary mediation of allelopathy, six target species were grown in a factorial experiment with and without activated carbon or a native allelopathic species, Solidago canadensis (Canada goldenrod). I used activated carbon to remove allelochemicals to separate allelopathic effects from resource competition. The species represented pairs of evolutionary experienced (native - N) and evolutionarily na'ive (non-native …

Effects Of Forest Edges On Population Dynamics In A Successional System, Kimberly Anne Lang

Masters Theses

Abandoned agricultural land is often bound by remnant woodlands which may locally influence successional dynamics. The primary objective of this study was to examine the effect of an old-growth forest edge on plant population dynamics within a successional system. Data from the Buell-Small Succession Study (BSS) in New Jersey were used to analyze population dynamics in relation to the forest edge during the first 50 years of succession following agriculture. It was hypothesized that edge responses would be driven by either differential colonization (dispersal and establishment) or differential performance (increase within plots).

By looking at individual species' edge responses, I …

Soil Variability And Its Influence On Plant Performance, Jeremy R. Klass

Masters Theses

During recorded history, nearly all temperate deciduous forests in eastern North America have seen extensive agricultural land-use conversions. Persistent influences of agricultural disturbance continue to shape the diversity and composition of the plant community long after agriculture has ceased. While some measures of ecosystem functioning may recover relatively quickly from agricultural disturbance, other characteristics such as levels of organic matter, carbon, and phosphorus, may remain different from primary forests for much longer periods. The alteration of the original ecosystem due to agriculture practices has far reaching impacts on biotic and nutrient dynamics along with causing significant shifts in community composition …

Population Ecotoxicology Of The Common Snapping Turtle In Agriculturally Impacted Lotic Ecosystems, Derrick L. Douros

Masters Theses

Anthropogenic impacts on lotic systems, including pollutants, have had both direct and indirect negative effects on the aquatic habitats of the world. Goals of this study are: (1) Quantify atrazine, the atrazine metabolite diaminochlorotriazine (DACT), and glyphosate burdens in common snapping turtle (Chelydra serpentina) tissue from individuals collected within the Embarras River in Coles County, Illinois. (2) Quantify the atrazine, DACT, and glyphosate loads in water from the aquatic habitats in which common snapping turtles reside. (3) Quantify the relative abundance of all turtle species and movement patterns of common snapping turtles in this lotic ecosystem. Hoop nets and modified …

Analysis Of Eurycea Hybrid Zone In Eastern Missouri, Bonnie Jean Beasley

Masters Theses

"Evolutionary mechanisms are often difficult to observe in action because evolution generally works slowly over time. Hybrid zones provide a unique opportunity to observe many evolutionary processes, such as reinforcement, because of the rapid changes that tend to occur in these zones. Salamanders provide an ideal model for examining the rapid changes in populations that result from hybridization because many closely-related species lack reproductive barriers. In Missouri, a well-documented hybridization zone exists among the two subspecies Eurycea longicauda longicauda (long-tailed salamander) and E. L. melanopleura (dark-sided salamander). These salamanders inhabit caves, limestone creek beds, and abandoned mine shafts. A closely …

Laryngeal Apparatus And Call Structure In North American Hylids, Barbara Ann Catherine Fears

Masters Theses

"Although the ecological implications and structure of frog calls have been the subject of much study, little is known about the association between the laryngeal apparatus morphology and call structure in North American hylids. In this study linear measurements of the laryngeal apparatus were captured and compared to the call structures of thirteen species of North American hylids. Species examined included: Pseudacris crucifer, P. triseriata, P. ocularis, Acris crepitans blanchardi, Hyla avivoca, H. cinerea, H. gratiosa, H. chrysoscelis, H. versicolor, H. squirella, H. femoralis, H. arenicolor, and H. eximia. Six homologous landmark points were identified, and the lengths between them …

Zinc Oxide Nanoparticle Disruption Of Store-Operated Calcium Entry In A Muscarinic Receptor Signaling Pathway, Hsiu-Jen Wang

Masters Theses

"The influences of ZnO nanoparticles on cellular responses to activation of muscarinic receptors were studied in Chinese hamster ovary cells expressing the human M3 muscarinic acetylcholine receptor. ZnO particles (20 nm) induced cytotoxicity in a time and concentration-dependent manner: following a 24 h exposure, toxicity was minimal at concentrations below 20 µg/ml but virtually complete at concentrations above 28 µg/ml. ZnO particles did not affect antagonist binding to M3 receptors or allosteric ligand effects, but increased agonist binding affinity while eliminating guanine nucleotide sensitivity. At a noncytotoxic concentration (10 µg/ml), ZnO increased resting [Ca²⁺]i from 40 to 130 nM without …

Fish Assemblages In Manistee River Tributaries: Longitudinal Distribution Analysis, Seasonal Variation, And Riparian Improvement Evaluation, Nicholas J. Gressick

Masters Theses

Sedimentation affects both stream physical and biological integrity. Improperly designed stream passage accompanied with sedimentation and altered hydrology can impede fish passage and reduce fish assemblage integrity. The purpose of this study was to: 1) quantify impacts of poorly constructed road stream crossings and eroding banks on fish assemblages, and 2) assess these sites as sediment sources and connectivity breaks on entire fish assemblages and individual fish species. Electrofishing was conducted during spring and fall 2004 and 2005. A total of 29 electrofishing reaches were sampled which included 5 road-stream and streambank restoration sites. Sickle Creek (1st order) had reduced …

A Community Ecology And Stable Isotope Study Of Plankton, Macroinvertebrate, And Fish Communities Surrounding Groundwater Vents In Lake Huron, Michigan, Thomas Garrison Sanders Jr.

Masters Theses

Extreme environments can create ecotones with uncharacteristic physicochemical conditions and ecological communities. Recently, several submerged sinkholes were discovered in Lake Huron where hypoxic groundwater that is rich with dissolved ions intrudes into surrounding lake water. Researchers have documented large growths of cyanobacteria and chemosynthetic bacteria surrounding these groundwater vents, but little is known if this production is utilized by upper trophic level organisms and how the environmental conditions affect local community density and diversity. My objectives were threefold: (1) I wanted to confirm the presence and describe the nature and extent of chemical and physical gradients in the sublacustrine sinkhole …

From In-Vitro To In-Vivo: Corporate Development And Efficacy Of A Topical Hair Growth Agent Derived From Natural Extracts, Kelly Michael Glynn

Masters Theses

Androgenetic alopecia (male pattern baldness) affects up to 50% of the world’s population, propelling the development for a possible treatment. The hair follicle is influenced by several genetic and physiologic factors, which, when gone awry, lead to androgenetic alopecia. Vascular endothelial and keratinocyte growth factors are believed to be promoters of hair growth, as is inhibition of the proteasome complex. The cytokine IL-1α is also known to regulate follicle dynamics. The research objective described herein was an attempt to develop a botanical blend, which could mediate the above biomarkers, be successfully incorporated into a safe topical product and be evaluated …

The Effect Of Coalmine Effluent On The Parasite Assemblages Of Lepomis Spp. In The Saline River Basin, Andrew Claxton

Masters Theses

To determine the efficacy of using parasite assemblages as bioindicators of coalmine effluent, a survey of gastro-intestinal helminths from the gut of sunfish, Lepomis spp, was performed. Five species of Lepomis, L. cyanellus, L. nwcrochirus, L. megalotis, L. microlophus, and L. gulosus, were collected from six locations near the Palzo mine, an abandoned coalmine that actively discharges into the lower reach of Sugar Creek. Sunfish were collected from three sites which did not receive effluent from the Palzo mine (Sugar Creek (37° 37'/ 88° 50'), South Fork(37° 41 '/ 88° 52'), and the Little Saline River (37° 38'/ 88° 38')) …

Feathers As Bioindicators Of Pcb Exposure In Clapper Rails, Jay W. Summers

Masters Theses

In this study we used feathers as a biomonitor for exposure to the polychlorinated biphenyl (PCB) Aroclor 1268. Clapper rails have been used as an indicator species of environmental damage for the LCP superfund site located in Brunswick, GA USA. LCP is contaminated with Aroclor 1268, which has been used in limited amounts elsewhere and therefore can be used as a contaminant marker. Aroclor 1268, including congener profiles, were quantified using gas chromatography (GC). Concurrently each sample was quantified for total Aroclor 1268 using an enzyme-linked immunosorbant assay (ELISA) and compared to the GC results to determine if ELISA was …

Behavioral Response Of Japanese Beetles (Popillia Japonica) To Sex Pheromone: Exploring Factors Of Social Situation And Recent Mating Experience, Carissa A. Schoenick

Masters Theses

Insects in have several methods of advertising for mates; one important and common method is via the use of chemical pheromones. Insects release pheromones into the environment to communicate information about their sex, mating status and receptivity to others. For example, Japanese beetles (Popillia japonica) produce a very volatile sex pheromone conspecifics can readily detect. This pheromone is released by virgin females only, and triggers scramble competition among the males that detect and follow the signal for access to these females. Because Japanese beetles are an invasive species and an important pest of ornamental and agricultural plants, there is strong …

Photopigments As Descriptors Of Phytoplankton Assemblages For Biotic Assessment Of Illinois Lakes And Reservoirs: An Hplc Aided Analysis, Robert J. Krenz Iii

Masters Theses

The Illinois Environmental Protection Agency (IEPA) and U.S. Environmental Protection Agency (USEP A) identify needs to monitor aquatic resources by quantitative means using biocriteria in conjunction with routine environmental assessment to identify sources of impairment, and determine the effectiveness of management protocols (IEP A 2007; USEPA 2007). Though bioassessment protocols have been implemented within lotic systems and lakes of natural origin, investigation of their application to functionally unique reservoir habitat, which dominates the Midwest, has been limited. This study .evaluated the utility of photopigment profiles, attained using high-performance liquid chromatography (HPLC), for development ofbioassessment protocols for Illinois lakes and reservoirs. …

Dynamics Of Aggregation Formation In Japanese Beetles, Popillia Japonica, Katelyn A. Kowles

Masters Theses

For most insect aggregations to form, they need to be started by an initial individual (the pioneer) andjoined by later individuals (the joiners). Pioneers and joiners may differ with regard to characteristics such as sex and body size. We carried out three field experiments to examine the characteristics of Japanese beetles, Popillia japonica, pioneering and joining aggregations on host plants. Individual beetles were captured as they arrived on uninhabited grape plants, as well as plants designed to simulate aggregations with model beetles and feeding damage. For all experiments and treatments, the beetles arriving were significantly female-biased, with pioneer females having …

Ecology And Impacts Of Lianas In Regenerating Forests, Laura Marie Ladwig

Masters Theses

Although they are important components of forest communities, the general ecology of temperate lianas, especially during forest regeneration, is largely unknown. The dependence of lianas on other plants for physical support makes them a potentially important driver of community dynamics. I examined current and past vegetation data from an old-field succession study in the Piedmont region of New Jersey, USA to determine the dynamics of liana expansion and their role in forest regeneration. Fifty years of vegetation data were utilized to determine community controls and population dynamics oflianas during expansion. Five lianas, Celastrus orbiculatus, Lonicera japonica, Parthenocissus quinquefolia, Toxicodendron radicans, …

Aging In The Mammalian Olfactory System, Stephen Clark

Masters Theses

The purpose of this investigation was to investigate genetic and age~related differences in the mammalian olfactory system. The molecule of interest is apolipoprotein E (apoE), a component oflipoprotein molecules which functions in the transport of cell membrane components (triglycerides, lipids, and cholesterol). Specifically in the nervous system, apoE is synthesized by glial cells that function in recycling dead nervous tissue components for delivery to growing or dividing neurons. ApoE is present in three protein isofon11s: apoE2, which has been associated with type III hyperlipoproteinemia; apoE3, rhe normal human isoforrn; and apoE4, which has been associated with Alzheimer's disease (AD). The …

Interaction Of Apoe With Estrogen In The Olfactory System During Nerve Maintenance And Recovery, Michael M. Tonsor Ii

Masters Theses

Estrogen replacement profoundly aids in nerve regeneration after injury in adult mammalian models. Estrogen replacement also protects against olfactory dysfunction in mammals. The mechanism underly1ng estrogen's neuroprotective effects remains ambiguous. My goal is to investigate and better clarify if apolipoprotein E (apoE ), a lipid transporting protein, is a critical intermediary to estrogen's effects on nerve maintenance and regeneration.

My hypothesis is that apoE is a mediator of estrogen's beneficial effects. This hypothesis is based on the following observations from previous studies. First, olfactory nerve regeneration was delayed in apoE-gene knockout (KO) mice as compared to wild type (WT) mice. …

Fragmentation Effects On Fitness In Five Common Prairie Species, Lydia Miramontes Loyd

Masters Theses

Midwest tallgrass prairies have suffered extensive habitat loss and as a result persist as isolated and highly fragmented remnants. In such small, isolated remnants, genetic drift and inbreeding depression can lead to a loss of genetic variation over time. Reduced genetic variation is often associated with reduced levels of growth, survival, and reproduction as well as increased susceptibility to stress. This study examined fitness traits of five common prairie species to determine whether plants in small fragmented remnants had reduced fitness as compared to those in a larger remnant. Five prairie species were chosen for this study based on their …

Nest Success, Nesting Habitat, & Nestling Feeding Rates Of Red-Headed Woodpeckers In East-Central Illinois, Nathan Cole Hudson

Masters Theses

The Red-headed Woodpecker (Melanerpes erythrocephalus) is a classic oak savanna species that has suffered significant population declines caused, in part, by extensive loss of savanna habitat and agricultural development. Despite consistent data that reveal a dramatic decline throughout much of their range, few studies have examined other possible explanations. In an effort to determine potential causes for their continued decline (as well as add to existing knowledge of nesting biology), we examined nest success and nesting micro-habitat features. We compared micro-habitat variables at: 1) golf and non-golf course sites, 2) successful and failed nest sites, and 3) nest and random …