Biology Commons^™

Isolation Of A Dosage Dependent Suppressor Using Saccharomyces Cerevisiae As A Model System, Rachel Chimner

Masters Theses

Human pathogenic Yersinia use a type three secretion system to deliver various effector proteins into host cells. Once these effector proteins are within the cell, they elicit a cascade of events that disrupt the normal immune response. One of these effectors, YopT, is known to disrupt actin distribution but it is currently unknown what YopT targets within the host cell. To investigate the cellular targets of the YopT effector, we use a yeast model system and a dosage-dependent suppression screen. The dosage-dependent suppression screen isolated three plasmids able to suppress YopT induced lethality within yeast. One of them, 2T9, was …

Broad-Spectrum Antibacterial Properties Of Metal-Ion Doped Borate Bioactive Glasses For Clinical Applications, Megan Ottomeyer

Masters Theses

"Bioactive glasses with antimicrobial properties can be implemented as coatings on medical devices and implants, as well as a treatment for tissue repair and prevention of common hospital-acquired infections such as MRSA A borate-containing glass, B3, is also undergoing clinical trials to assess wound-healing properties. The sensitivities of various bacteria to B3, B3-Ag, B3-Ga, and B3-I bioactive glasses were tested. In addition, the mechanism of action for the glasses was studied by spectroscopic enzyme kinetics experiments, Live-Dead staining fluorescence microscopy, and luminescence assays using two gene fusion strains of Escherichia coli.

It was found that gram-positive bacteria were more …

Production Of 1,3-Propanediol From Glycerol Under Haloalkaline Conditions By Halanaerobium Hydrogeniformans, Daniel William Roush

Masters Theses

"With increased demands around the world to make modern lifestyles more environmentally friendly, the chemical commodity market has rapidly shifted. Through new technologies in chemical production, certain high value products have oversaturated the market and have become high-volume, low value waste products. The expansion of biodiesel production offers a prime example; high volumes of glycerol byproduct from this process have shifted glycerol from a high priced commodity to a common waste product. A number of microorganisms are known to synthesize the polymeric precursor 1,3-propanediol from glycerol; however, crude glycerol from biodiesel production creates a harsh environment for most microbes, and …

Wetting And Drying Cycles And The Fungal Communities On Leaf Litter In Streams, Kele Qwinn Thrailkill

Masters Theses

"Leaf litter is a major source of energy for streams in deciduous forests. Fungi play a critical role by converting the leaves into nutritional material for the rest of the food web. The breakdown of leaf litter and associated biota, including invertebrates and fungi, have been proposed as measures of stream health in systems affected by anthropogenic activity. Rates of leaf breakdown can be depressed in streams affected by acidity, metals, organic contaminants, and other stressors. Climate change may lead to alterations in stream hydrology such that streams experience more frequent floods as well as drying episodes. Fungal communities can …

Laboratory And Field Performance Of The Biosand Point Of Use Water Filtration System In The Artibonite Valley, Haiti, Andrew John Sisson

Masters Theses

The research presented here is summary two years of studying the Biosand filter (BSF) both in Haiti during March 2011 and in the laboratory. In Chapter 2, we examined the long term use and sustainability 55 BSF systems near Deschapelles, Haiti and 47% were found to be no longer in use. Interviews with BSF owners revealed problems related to intermittent filter use. A review of 17 BSF field studies also was included to compare and substantiate observations made in Haiti. Together, previous field studies and our observations point toward the importance of providing culturally appropriate technologies and education materials explaining …

The Rise And Fall Of Kin Structure In The Neotropical, Family-Living Buff-Breasted Wren (Cantorchilus Leucotis) In Gamboa, Panama, Sarah C. Alessi

Masters Theses

Family-living species provide an exciting model to examine how natal dispersal and kin structure influence genetic structuring within local populations. Juvenile buff-breasted wrens (Cantorchilus leucotis) of both sexes delay dispersal and exhibit short-distance natal dispersal, which should lead to kin-structured populations in which relatives of both sexes occupy neighboring territories. Blood samples collected from juvenile and adult wrens in Gamboa, Panama were analyzed using microsatellite markers to determine whether related individuals are spatially clustered on neighboring territories, spatial clusters of relatives change over time, and if kin structure is sex-specific. Global and local spatial autocorrelation analyses detected genetic …

Origin Of Great Lakes Brown Trout, Salmo Trutta: A Phylogeographic Analysis Using Mtdna Sequence Variation, David Baisch

Masters Theses

The brown trout, Salmo trutta, was first introduced to the Laurentian Great Lakes in 1887 from European broodstocks to found a recreational salmonid fishery; however, the origins of these progenitor lineages remain largely unknown. Trout from these regions are very specialized to their native habitats and matching North American stocks to similar watersheds may help increase survivability by introducing the stock to a more appropriate environment. The objective of this study was to determine the European origins of brown trout found in the Great Lakes. We analyzed 144 brown trout from ten watersheds across Michigan and Wisconsin and identified …

Winter Habitat Selection And Genetic Relatedness Of Southern Flying Squirrels In Southwest Michigan, Sheila M. Miara

Masters Theses

Few studies have examined winter habitat selection for southern flying squirrels (Glaucomys volans) in northern latitudes where winter conditions are harsher than southern latitudes and resource availability is particularly limiting. Winter den tree characteristics and use by 19 southern flying squirrels (10 females and 9 males) were investigated from November through February in 2010-2011 and 2011-2012. The mean home range size using 100% minimum convex polygon for 18 individuals was 3.40 ha (±0.46 0.46 SE) and there was no significant difference between genders (P= 0.56) or ages (P=0.50). The mean 95% fixed kernel home range size was 0.15 …

Effects Of Ailanthus Altissima Soil Leachates On Nodulation And Expression Of Two Genes That Regulate Nodulation Of Trifolium Pratense, Jesse Michael Lincoln

Masters Theses

Ailanthus altissima (Simaroubaceae) is an invasive tree from China that has spread over much of North America. A number of characteristics contribute to its success, notably tolerance of nutrient-poor soils, rapid growth rates, prolific production of wind dispersed seeds, escape from predation, and the production of allelopathic compounds. That Ailanthus altissima frequently co-occurs with legumes in nutrient poor soils alludes to a possible relationship between Ailanthus altissima and nitrogen fixing legumes. Legumes increase the biospheres supply of bio-available nitrogen through facilitating the fixation of atmospheric nitrogen within structures on roots called nodules. Thus, increased nodulation of legumes caused by Ailanthus …

Demographics And Seasonal Abundance Of Sportfish In Three Thermal Habitats Of Coffeen Lake, Anthony Paul Porreca

Masters Theses

Power generating facilities across Illinois provide electricity to residents of the state, while at the same time offering desirable open water fishing lakes that can be used by anglers during the winter season when other reservoirs are not accessible. Coffeen Lake represents one of the best power cooling fisheries in the state and boasts both excellent largemouth bass and channel catfish populations. The reservoir supplies water to a power station with a generating capacity of 945 megawatts of electricity, with 73% of the surface water affected by heated discharge and a cooling loop covering 4.1 miles. Sampling sport fish species …

Population Demographics Of White Crappie In Three Illinois Reservoirs, Candice Marie Miller

Masters Theses

Crappie provide important sport-fisheries throughout the United States. Because of the propensity of this species to display growth stunting, an assessment of crappie population demographics is needed in Midwestern reservoirs. I assessed the population demographics of white crappie, Pomoxis annularis, in three Illinois reservoirs. Samples were collected by three-phase AC boat electrofishing. All crappie were weighed, measured, and the otolith and scale samples were removed for age estimation. Relative density, as measured by catch per unit of effort (CPUE; fish/hour), was different at Lake Mattoon (0.53±0.15), Lake Charleston Side Channel Reservoir (1.17±0.15), and Paradise Lake (1.04±0.18). Size structure of white …

Feeding Behavior Of Nestling Eastern Bluebirds (Sialia Sialis) In East-Central Illinois, Amanda Lamb

Masters Theses

I observed feeding of nestlings in Eastern Bluebirds in nest boxes in east-central Illinois. Provisioning rate of males and females were compared and early observation periods (younger nestlings) were compared with late observation periods (older nestlings). I also compared the average food size brought to the nestlings by males and females and during early and late observations. No significant differences were found between average food sizes throughout the study. In addition, food size was not significantly correlated with mean nestling weights within a brood. However, males had significantly fewer confirmed feedings than females for the last observation period. In addition, …

Linking The Colonization Rate Of Vesicular Arbuscular Mycorrhizae (Vam) To Species Dynamics In Succession, Jie Luo

Masters Theses

Vesicular arbuscular mycorrhizae (YAM) are the most common mycorrhizal fungi that form symbiotic associations with plant species. They form hyphae, vesicles and arbuscules which expand root surface area and exchange nutrients within root cells. Greater abundance of YAM can result in more nutrient uptake and disease resistance to the host plant. While mycorrhizae are often important to the growth and reproduction of individual species, their role in regulating plant community dynamics is less clear. Previous research has shown that YAM are tightly related to plant traits such as relative growth rate, foliar N and P concentrations, and responses to soil …

Effect Of Reservoir Discharge And Sanitary Effluent On An Urban Stream, Daniel Hiatt

Masters Theses

Eutrophication of aquatic ecosystems may occur from several sources, primarily from anthropogenic inputs. Excess nutrient deposition can result in eutrophication and community compositional shifts in organisms located in impacted ecosystems. In the Sangamon River located outside Decatur, IL, discharge from the local reservoir, which receives inputs from both a city landscape and agricultural landscape and a wastewater treatment plant (WWTP). These may result in a large influx of nutrients into this system. Increased nutrient levels have the potential to shift benthic periphyton, and macroinvertebrate communities and cause extinction of low tolerance native species. To examine the impacts of inputs for …

Analysis Of A Wound-Induced Gene Family In Glycine Max, Gena Robertson

Masters Theses

Gene families in plants are important in understanding genome evolution indicating when and where genome duplications and segmental duplications occurred as well as subsequent divergence and subfunctionalization. A gene family in Glycine max that encodes a WI12 protein, wound-induced protein, was found to consist of ten genes on five chromosomes. Wound-induced proteins are activated in response to wounding in plants, and the WI12 protein in particular is thought to be involved in cell wall modifications at the wound site. A variety of bioinformatics tools have been used to analyze the expansion of this family in soybean as well as identify …

Whatchu Breathin'?: Sick Building Syndrome And Seasonal Occurrence Of Fungi, Jessica Stapleton

Masters Theses

This study was designed to evaluate the presence of sick building syndrome in a classroom at Unity High School (Mendon, Illinois) and contrast differences in the number of fungal spores per cubic meter of air in different rooms in the building and between seasons. Air and surface samples were taken to analyze the number of fungal spores per cubic meter and to evaluate generic fungal diversity. Factors influencing the number of fungal spores per cubic meter and genera of fungal isolates were year-round temperature control in the classroom and the presence or absence of students. Some of the fungi isolated …

Bioavailability, Bioaccumulation, And Trophic Transfer Of Trace Metals In The Tims Branch-Steed Pond Tributary, Paul Gethyn Edwards

Masters Theses

The Department of Energy's (DOE) Savannah River Site (SRS) is a 777 km² former nuclear weapon material production and current research facility located along the Savannah River in west-central South Carolina. In 1954, direct environmental discharges were released from "M-Area", a fuel and aluminum-clad Uranium (U) nuclear reactor target manufacturing facility through a drainage ditch into the Tims Branch-Steed Pond water system. The wastewater discharges released into Tims Branch contained large volumes of inorganic wastes including depleted and natural U, aluminum (Al), and Nickel (Ni) along with smaller quantities of mercury (Hg), lead (Pb), copper (Cu), chromium (Cr), and …

Egg Recognition And Cowbird Egg Ejection Behavior In The American Robin (Turdus Migratorius), Allison Karlien Lang

Masters Theses

American Robins (Turdus migratorius) typically eject parasitic cowbird eggs from their nests. In order to successfully remove parasitic eggs, robins must first be able to differentiate between their own and foreign eggs and then remove the unwanted egg(s). This study addressed these behaviors. A robin's ability to recognize its own eggs was tested by adding artificial cowbird eggs to robin nests at various parasite-tohost egg ratios which created three treatments: (1) a majority of robin eggs in the nest, (2) an equal number of robin and cowbird eggs, and (3) a majority of cowbird eggs. At 89% of nests (51 …

Comparison Of Silver Carp Population Demographics In An Impounded And A Free Flowing River, Jason G. Stuck

Masters Theses

The silver carp, Hypophthalmichthys molitrix, is an invasive species that has received increased attention due to the proximity of the population in the Illinois River to the economically important Great Lakes and the species ability to dominate the aquatic biomass of an ecosystem. We sought to assess the population demographics of silver carp on two different river ecosystems, the Illinois River and Wabash River. The Illinois River is channelized and dammed for commercial barges, whereas the Wabash River is the longest free flowing river east of the Mississippi River and is still connected to its natural flood plain. Silver carp …

In Vivo Evaluation Of Microfibrous Bioactive Borate Glasses For Use In Wound Healing, Yinan Lin

Masters Theses

"This project consisted of two parts focused on in vivo evaluation of microfibrous bioactive borate glasses for use in wound healing. Part one of the project involved subcutaneous implantation of two types of borate glass microfibers and 45S5 glass micro fibers in the dorsum of rats and histologically evaluating the soft tissues at the implant sites at 2 to 4 weeks post surgery. The primary objective was to assess the angiogenic response to the implanted materials. The 13-93B3 and 13-93B3Cu glass microfibers were found to significantly increase the microvascular density in soft tissue, an outcome that suggests these simple and …

Physiochemical Characteristics Contributing To The Cytotoxicity Of Transition Metal Oxides, Chi-Heng Wu

Masters Theses

"In the past ten years nanotechnology has not only evolved to play a prominent role in our economy but also increased the concern over potential adverse effects caused by nanomaterials to human health and the environment. Nanotoxicity is to understand the nature and origin of the toxicity imposed by nanomaterials. Studies from our laboratory have shown that nanoparticle induces oxidative stress, perturbs calcium homeostasis, alter gene expression, and produces pro-inflammatory responses. We also identified a trend of toxicity: TiO₂ < Cr₂O₃ < Fe₂O₃ < Mn₂O₃ < NiO < ZnO < CuO. We then asked a question: what are physiochemical factors of transition metal nanoparticles that contributed to this increasing cytotoxicity. In this thesis I investigated the correlation between physicochemical properties and toxicity of the transition metal oxides in the 4th Period of the Periodic Table of Elements. Particle size, BET surface area, point of zero charge, metal dissolution, and degree of surface adsorption of transition metal oxide nanoparticles were measured. There were no increasing trends in both particle size and specific surface area. The point of zero charge showed an increasing trend as TiO₂ < Cr₂O₃ < Fe₂O₃ < NiO < CuO = ZnO < Mn₂O₃. The number of available binding sites of nanoparticle showed an increasing trend as Cr₂O₃ > ZnO > CuO > NiO > Fe₂O₃ > Mn₂O₃ > TiO₂. The degree of adsorption on the surface of nanoparticles showed an increasing trend with atomic …

Social Dimensions Of Lake Ecology: Stakeholder Perception Surveys Used To Design Effective Lake Management Plans, Laurie Nannini

Masters Theses

Lake conservation management plans have exhibited varying levels of success through out Illinois. Recent research, in eutrophic lakes shows that pollution at any level stabilizes when stakeholder cooperation is enhanced by high conformist tendency and social concern. Involving stakeholders in preliminary stages of lake management plans creates more legitimacy and connectedness with the local government as well as stakeholder involvement with management plans. Public opinion data also allows policy makers to sustainably involve stakeholders in management plans. Members of the Illinois Lake Management Association were surveyed on their perceived threats to lakes, preferences on lake facilities and activities. Responses were …

Is Anthropogenic Habitat Change The Driving Force Of Rapid Evolution Of Southeastern U.S. Coastal Deer Populations?, Nicole L. Storm

Masters Theses

Many factors, such as genetics, food availability and the exact food species, can affect the growth rate and asymptotic size of animals. Since the I 960's, the coastal areas and islands in Georgia, North Carolina, and South Carolina have become vacation destinations with large and lavish resort communities built to accommodate the large number of visitors to areas that were once called home by just a small number of residents. These developments brought with them non-native plants and improved soil quality for both native and non-native plants. During this same time period, the whitetailed deer ( Odocoileus virginianus) native to …

Isolation And Characterization Of Bacterial Symbionts From Crotalaria Spectabilis Grown On Trichloroethene Contaminated Soil, April Lyn Rocha

Masters Theses

"The discovery of several members of the Leguminosae family growing on a TCE contaminated site demonstrated that they have the capabilities to tolerate the TCE contamination. This led to an investigation of the plants' ability to metabolize TCE. Research by Dr. Lee Newman showed that nodules from Crotalaria spectabilis grown on the site had TCE metabolites present while nodules from other legumes at the site did not. The presence of TCE metabolites within the C. spectabilis nodules suggests that the bacterial symbiont may be responsible for TCE metabolism. This led us to investigate the symbiont of C. spectabilis and its …

Analysis Of Extinction Of Fibroblast-Specific Gene Expression In Somatic Hepatic Hybrids Using Cdna Microarray Technology, Sushma K. Shrestha

Masters Theses

Extinction of tissue-specific genes in mammalian somatic cell hybrids is a well known epigenetic phenomenon and knowledge of this process is useful in understanding molecular mechanisms underlying tissue specific gene expression. Signaling proteins critical for the activation of transcription including transcription factors, co-activators, and repressors, play a role in the establishment and maintenance of tissue-specific phenotypes. Tissue-specific transcription factors are the key driving force of tissuespecific gene expression. However, mechanisms responsible for gene silencing are largely unknown.

Extinction of liver specific traits in somatic hybrids generated by the fusion of hepatoma and fibroblast cells has been well documented and is …

Interactions Of Diet And Behavior In A Death-Feigning Snake (Heterodon Nasicus), Andrew Michael Durso

Masters Theses

Studies of animal behavior in captivity are limited in their ability to explain the influence of a natural environment on behavioral ecology. Defensive behaviors vary among individual animals, between sexes and with age, as well as with other less well-known factors. The toxin-rich diet of many toad-eating snakes might enable or cause their passive terminal defensive behavior of death-feigning. I videotaped death-feigning behavior in wild Plains Hog-Nosed Snakes (Heterodon nasicus) subjected to standardized harassment. Using stable isotopes, I also described the diet of the same individuals subjected to behavioral analysis (n = 40). Heterodon had broader diets than other snakes …

Estrogen Promotes Neurite Outgrowth In Olfactory Epithelial Explant Cultures Through The Estrogen Receptor, Apryl E. Pooley

Masters Theses

No abstract provided.

A Functional Genomics Approach To Analyze Two Methionine-[Gamma]-Lyase Orthologs In "Ferroplasma Acidarmanus" Using In Vitro Enzyme Assays, Md. A. Wadud Khan

Masters Theses

Functional genomics predicts that ferl genome has two candidate genes for the production of two methionine-y-lyase (MGL) orthologs, which are thought to be pivotal for the production of methanethiol. These orthologs show a high level of protein sequence identity ( ~ 3 5%) and similarity (~60%) when compared to known MGLs. In addition, ClustalW2 alignment of ferl MGL orthologs and other known MG Ls showed a high level of similarity in amino acid residues, which are involved for the stabilization of 3D structure of MGL and in forming active site for the substrates. To confirm the predictive functions of these …

The Evolution Of Leaf Anatomy In Badiera (Polygalaceae), Laura Brauer

Masters Theses

Leaf samples from Polygalaceae: nine species of Badiera, two species of Bredemeyera, three species of Hebecarpa, and two species of Polygala were taken from herbarium specimens for anatomical observations. Several methods of rehydration were compared to a control of fresh material fixed in the field for four species of Badiera. Soaking in concentrated ammonium hydroxide proved to be the best overall means of rehydrating leaves of the genus Badiera and related genera. All leaves were embedded in paraffin for sectioning and cleared in 5% aqueous sodium hydroxide for examining epidermal features. Twelve anatomical characters were mapped onto a molecular phylogeny …

Mutation Of The Hof1 Pest Domain Affects Cytokinesis In Budding Yeast, Katherine E. Stockstill

Masters Theses

"Cytokinesis is the division of a cell into two daughter cells. Cytokinesis failure results in polyploidy, which may contribute to tumorigenesis or cause cell death. The Saccharomyces cerevisiae protein Hof1, which is required for efficient cytokinesis, is phosphorylated during mitosis. The hypothesis that MEN (mitotic exit network) dependent phosphorylation regulates Hof1 degradation, triggering completion of cytokinesis was tested. Hof1 contains a PEST sequence, which is rich in proline, glutamic acid, serine, and threonine, and is believed to be involved in protein degradation. To test the hypothesis, we created two non-phosphorylatable mutants of the Hof1 PEST domain tagged with GFP. The …