Biology Commons^™

Nest Success, Nesting Habitat, & Nestling Feeding Rates Of Red-Headed Woodpeckers In East-Central Illinois, Nathan Cole Hudson

Masters Theses

The Red-headed Woodpecker (Melanerpes erythrocephalus) is a classic oak savanna species that has suffered significant population declines caused, in part, by extensive loss of savanna habitat and agricultural development. Despite consistent data that reveal a dramatic decline throughout much of their range, few studies have examined other possible explanations. In an effort to determine potential causes for their continued decline (as well as add to existing knowledge of nesting biology), we examined nest success and nesting micro-habitat features. We compared micro-habitat variables at: 1) golf and non-golf course sites, 2) successful and failed nest sites, and 3) nest and random …

Analysis Of Transcription Factor Promoter Binding In Rat Hepatoma/Fibroblast Hybrids, Jordan C. Angle

Masters Theses

When hepatomas (liver cancer cells) are fused with fibroblasts (undifferentiated body cells), there is complete extinguishing of all tissue-specific gene expression. Once it was discovered that many hybrid cell lines do not express needed transcription factors for liver-specific gene transcription, the factors were reintroduced and forced to express through the use of plasmids. Even if the two primary transcription factors were present in the system, downstream tissue-specific gene expression was not rescued. To examine potential mechanisms for the repression of liver-specific gene expression in hepatoma x fibroblast hybrid cells, chromatin immunoprecipitation (ChlP) was used. This procedure allows the liver-specific gene …

Long-Term Assessment Of Predatory Fish Removal On A Pond-Breeding Amphibian Community In Central Illinois, Lee M. Gross

Masters Theses

Habitat loss and fragmentation are important contributors to population declines in many species, so maintaining suitable habitat has become a priority for wildlife management. Removal of introduced fish can help restore suitable habitats for amphibian populations, although long-term assessments of such removals are lacking at the community level. In 2001, drift fences and pitfall traps were constructed around the majority of four ponds to monitor an amphibian community in a nature preserve in central Illinois. Rotenone™ was applied to ponds containing introduced fish in 2001, and again, in 2003. I collected data from 2005-2007 to determine the long-term impacts of …

Habitat Selection And Niche Partitioning Of Fox And (Sciurus Niger) And Gray (Sciurus Carolinensis) Squirrels In Charleston, Il, Katelyn Marti

Masters Theses

Two species of tree squirrels inhabit Charleston, IL; fox squirrels (Sciurus niger) and gray squirrels (Sciurus caro/inensis). Although these species are sympatric throughout much of the eastern U.S., urban settings are usually occupied by one or the other. In the few localities where they co-occur, little is known about how they partition habitat. Therefore, the objectives of my study were to determine: (1) the relative abundance of each species, (2) whether habitat use differs between species, (3) if habitat use shifts with season, time of day, domestic predator abundance, or mast availability, and (3) observe interspecific and intraspecific interactions. The …

Survival Strategies Of The Endangered Physaria Ludoviciana (Silvery Bladderpod; Brassicaceae), Marissa Catherine Jernegan Grant

Masters Theses

Physaria ludoviciana (Nuttal) O'Kane and Al-Shehbaz is an endangered species in Illinois and Minnesota sand prairies, but has many individual populations in western states. Areas where P. ludoviciana occurs have high summer temperatures, a sandy soil with low water holding capacity and high drainage, the frequent disturbance of blowing sand and little canopy vegetation, giving it a full sun environment. This habitat is where few other plants are able to establish, providing P. ludoviciana with little competition. Physaria ludoviciana exists in a niche where it is adapted and able to establish and persist. The focus of this study was to …

Electricity Generation And Ethanol Production Using Iron-Reducing, Haloalkaliphilic Bacteria, Varun Paul

Masters Theses

"Microbial life in extreme environments has been studied primarily for their metabolic activities. Very few commercial or industrial applications have been known from these systems. In this study, the metabolic pathways and properties of bacteria from a haloalkaliphilic environment of Soap Lake, Washington were employed in two research applications related to energy production. In the first study, the bacterial cultures that were known to reduce iron (III) were used in a Microbial Fuel Cell (MFC) reactor. Iron reducing bacteria have shown to shunt their electrons on to the surface of a carbon electrode of an MFC, and the electrons can …

Nona-Arginine Peptides Facilitate Cellular Entry Of Semiconductor Nanocrystals: Mechanisms Of Uptake, Yi Xu

Masters Theses

"Luminescent semiconductor quantum dots (QDs) have recently been used for delivering and monitoring biomolecules, such as drugs and proteins. However, QDs alone have a very low efficiency of transport across the plasma membrane. In order to increase the efficiency of QD delivery, synthetic nona-arginine (sR9) was used, a cell penetrating peptide, to facilitate uptake. Data demonstrated that sR9 could significantly increase the cellular uptake of QDs by noncovalent binding between QDs and sR9. Furthermore, the mechanisms of QD/sR9 cellular internalization were investigated. Low temperature and metabolic inhibitors markedly inhibited the uptake of QD/sR9, indicating that internalization is an energy-dependent process. …

In Vitro Evaluation Of Bioactive Glass Scaffolds And Modified Bioactive Glasses With An Osteogenic Cell Line, Vernon C. Modglin

Masters Theses

"This investigation consisted of two parts focused on bioactive glasses and scaffolds for in vitro growth of bone cells for tissue engineering and bone repair. The first part involved evaluation of the ability of two new types of porous 13-93 bioactive glass scaffolds to support the growth and differentiation of mouse MLO-AS cells, an established osteogenic cell line used as a model of developing bone tissue. The two scaffold types tested included 13-93 glass fiber and trabecular-like scaffolds seeded with MLO-AS cells and cultured for intervals of 2 to 12 days. Collectively, in vitro results indicate that the 13-93 glass …

A Genetic Assessment Of The Red Squirrel In Illinois: Natives, Immigrants, Or Exotics?, William Beatty

Masters Theses

A small population ofred squirrels (Tamiasciurus hudsonicus) has existed in Iroquois County, Illinois since the 1970' s. The origin of this population is unknown, but some accounts suggest they are the offspring of released red squirrels from Minnesota. Alternatively, they may be a remnant Illinois population or more recent immigrants from Indiana. Finally, this population could reflect a mixed ancestry of the above scenarios. Mitochondrial DNA (mtDNA) was utilized to determine the genetic relationships among red squirrel populations in Illinois, Indiana, Michigan, Minnesota, and Wisconsin. My results showed low levels of haplotype diversity and nucleotide diversity in the Illinois sample. …

Beneficial Effects Of Estradiol Are Mediated Through Apoe, Aseem Hussain

Masters Theses

Estradiol is the primary premenopausal hormone circulating in women. It is a powerful hormone in the female body since it sends signals to the brain, regulates ovulation and menstrual cycle and acts on the nervous syste~ heart, breast and many other tissues in the body. However all estradiol production is stopped after menopause. Post menopausal women can develop a host of neurodegenerative diseases such as Alzheimer's disease. If the onset of progressive dementia is after menopause during loss of estradiol, it seems logical to assume that the lack of estradiol should have a big role to play in the pathogenesis …

Tracing Sperm In Multiply-Mated Female Anastrepha Suspensa, (Diptera:Tephridae), Preeti Dhakal

Masters Theses

Anastrepha suspensa females have four different sites of sperm storage in their reproductive tract, may mate with more than one male, and store sperm in differing amounts in each organ after copulation. In order to study the dynamics of sperm storage patterns and subsequent paternity outcomes, a method was developed for both genotyping and quantifying sperm in the spermathecae. Spermathecae were isolated and sonicated to remove maternal cells, and subsequently crushed between two microslides to release sperm from the capsule. After DNA isolation, microsatellite loci could be amplified by PCR for fragment analysis. A newly developed method was then used …

Artificial Riffles As A Stream Remediation Technique, Bethany S. Harrington

Masters Theses

No abstract provided.

Influence Of Seed Source On Vegetative And Reproductive Performance Of Three Common Prairie Grasses Common In Grassland Restoration, Matt Burmeister

Masters Theses

Though tallgrass prairie once covered 250 million acres of the midwestem U.S, now less than one percent· of this original area is left. In recent years the restoration and reconstruction of tallgrass prairie has become more popular throughout the prairie region. There is evidence that local and distant populations may both be practical seed sources for ecological restoration projects. To address this basic issue in restoration, I tested the how performance of three grass species from different locations was affected by the distance from the restored area. Three species, Andropogon gerardii, Sorghastrum nutans and Panicum virgatum, of perennial grasses common …

Influence Of Fluvial Geomorphology On Fish Assemblage Structure Within An Agriculturally Impacted Watershed, Jeffrey D. Fore

Masters Theses

Warmwater stream fish assemblages are often considered limited by instream habitat availability. Thus, much current research has focused on determining habitat features that limit the abundance and persistence of stream fishes. These studies provide valuable information to managers who are concerned with increasing species abundance and persistence, but they may not provide information on the processes structuring the stream fish community. To address this issue, we hypothesized that stream geomorphology differed among stream sizes and types (i.e., channelized vs. unchannelized streams) and that the fish assemblages associated with each geomorphic type would be unique. Fish assemblages were sampled and geomorphic …

Long-Term Functional Trait Dynamics In Abandoned Agricultural Fields, Timothy A. Rye

Masters Theses

A fundamental question of plant succession is whether species within different successional stages differ in terms of functional traits as changes in community-level traits reflect changes in plant strategies over time. Although there are many studies that focus on that question, researchers have not studied the long-term dynamics of those traits within plant communities in depth. We used data from a 49-year study of old-field succession (the Buell-Small Succession Study) to assess the dynamics of important functional traits (height, seed mass, specific leaf area) and compared their dynamics among life forms and between native and non-native species. We assessed communitylevel …

Foraging Site Selection In Prairie Kingsnakes (Lampropeltis C. Calligaster): An Experimental Approach, Angela M. Fornell

Masters Theses

A critical component to a predator’s survival is the choice of foraging sites that yield enough predatory success to meet energetic demands. To elucidate cues used to select foraging sites, I examined foraging site selection in Prairie Kingsnakes (Lampropeltis c. calligaster), a generalist species whose foraging mode is flexible. My objectives were to determine: the pattern of foraging site selection; the importance of certain cues that kingsnakes use when selecting foraging sites; and, whether or not kingsnakes adjust their foraging patterns in response to alterations in the microhabitat. I tested subjects in a large experimental arena under several treatment conditions …

Terrestrial Macrofungi Of Old-Growth Prairie Groves, Vincent P. Hustad

Masters Theses

The purpose of this study was to evaluate and contrast the communities of terrestrial macrofungi of Brownfield and Trelease Woods, Champaign Co., IL; old-growth prairie groves isolated for> 120 years. This study represents the first known analysis of fungal communities in old-growth prairie grove ecosystems in the Midwestern United States, and provides an excellent opportunity to characterize the fungal biodiversity of established old-growth reference communities for comparisons with other sites, both local and distant. Fungal communities were sampled during the fall fruiting seasons of 2006 and 2007 using a plot frequency study of fungal abundance. Several estimators of species richness …

Correlating Phytoplankton Assemblages With Water Quality In Illinois Lakes And Reservoirs: Validating Models Based On Historical Data, Jason D. Allen

Masters Theses

In the last two decades management and monitoring efforts in freshwater systems has shifted from point source pollutants to non-point source pollutants and environmental modification. Long term, large scale sampling projects necessitated by such a shift often are infeasible due to logistical, cost, and time constraints.

In recent years fish and benthic macroinvertebrate indices of biological integrity (IBI's) have been used to survey the quality of flowing (lotic) water systems, which have drastically reduced sampling time and facilitated large scale monitoring projects. However, it is unlikely that these could be adapted to lentic systems. This study was undertaken in an …

The Impact Of Climate On The Leaf Characteristics Of Genus Fraxinus, Brian W. Craven

Masters Theses

Fraxinus (ash) has six species across eastern North America, five of which are found in Illinois (F. americana, F. pennsylvanica, F. nigra, F. quadrangulata, F. profimda). It is also threatened by the emerald ash borer (Agrilus planipennis) (BAB). Are climate differences across a north-south distance of 600 km great enough to create differences in the morphological characteristics of Fraxinus species? Studying latitudinal variation in the species of Fraxinus would reveal the impact of climate on the leaf characteristics of a genus of trees which extend over a considerable portion of the continent. The objectives of this study were to determine …

Evaluation Of The Oxalate-Consuming Activities Of Probiotic Microorganisms, Alexandra E.C. Baluka

Masters Theses

This study identifies the oxalate-consuming capabilities of mixtures of probiotic microorganisms, present as commercial probiotics, as well as in seventeen pure cultures of probiotic microorganisms. Probiotics are sold commercially as powders, tablets, and supplements of live microorganisms that, when taken in large amounts, confer a health benefit to the host. Increased levels of urinary oxalate coincide with the formation of kidney stones, so this study sought to identify organisms capable of oxalate consumption that could potentially be manufactured for those suffering from calcium oxalate kidney stones. This study determined that consumption of oxalate was not directly tied to active growth …

Mating Bias In The Red Imported Fire Ant, Solenopsis Invicta, Kendra Carroll

Masters Theses

The two social forms in the red imported fire ant, Solenopsis invicta, are believed to be governed by the expression of one specific gene. This gene, known as Gp-9, is responsible for the production of general protein-9, thought to be a pheromone binding protein. Among the social aspects thought to be controlled by this locus is reproduction. Many aspects of breeding within and between the two colonial forms have been investigated over the past years since in introduction of this species to the United States. In this study, several features of the currently accepted model were examined. Newlymated queens were …

Evaluating The Illinois Stream Valley Segment Model As An Effective Management Tool, Stephen S. Warrner

Masters Theses

Stream habitat assessment procedures have been used to monitor logical potential, habitat restoration, and determine anthropogenic impacts on streams. In the past, the Illinois Department of Natural Resources (IDNR) and the Illinois Natural History Survey (INHS) have determined which stream reaches to sample habitat quality based on historical data, accessibility, and whether or not a reach is representative of an area. To develop a consistent and unbiased procedure for choosing sampling locations, the IDNR and INHS have proposed a technique by which watersheds are divided into homogeneous stream segments called valley segments. Valley segments within each watershed are determined by …

Spatial And Ecological Patterns Of Tent Location In The Eastern Tent Caterpillar, Malacosoma Americana (Lepidoptera:Lasiocampidae), Jennifer Pleasant

Masters Theses

The eastern tent caterpillar, Malacosoma americanum (Fabricius) (Lepidoptera: Lasiocampidae) is a widely studied social caterpillar. The nests (i.e. tents) of the tent caterpillar are most frequently found on Black Cherry trees (Prunus serotina). However, dispersions of tents are often clumped with one cherry tree having five or more tent caterpillar nests in it and another cherry tree 3 meters away having only one nest, or none at all. I tried to determine why this happens. Because of the short field season, much of the research on the caterpillars has been done in the laboratory. I tried to determine host selection …

A Survey Of Carrion And Burying Beetles (Coleoptera:Silphidae) In Mchenry County, Illinois, David Suhrbur

Masters Theses

This study compared the occurrence of beetles in the family Silphidae using historical collection records with that of field collections from five different sites within McHenry County. The five sites include: Glacial Park, Woodstock, Silver Creek, Rush Creek, and Coral Woods. Historically, 11 known silphid species have been found in McHenry County, compared with seven species found during this study' collection period.

Percentages of silphid species at each site was calculated, as well as total number of individuals sampled, and two indices of diversity (Shannon-Weiner and Simpson's) were applied to both current and historical collection data. The historical collection (when …

The Identification And Characterization Of Phenylalanine Ammonia-Lyase Gene Family Members In Glycine Max, Erin Kathleen Pringle

Masters Theses

"Gene families are collections of genes with similar functions. Studying gene families is important for understanding the evolution of genes and manipulating genes. Phenylalanine ammonia-lyase (PAL) is an enzyme found in plants. It catalyzes the deamination of phenylalanine to produce cinnamic acid. Genes for PAL have been identified in many different plant species. This project used the known sequence for the PAL1 gene in Glycine max to find other PAL genes in Glycine max"--Abstract, page iii.

Role Of Microtubules In Budding Yeast Cytokinesis, Su Young Park

Masters Theses

"Cytokinesis is an important step to finish cell cycle events and separate into two daughter cells. This event is driven by actomyosin ring contraction. In budding yeast, the mitotic exit network (MEN) controls completion of ana/telophase by signaling events. Bub2 is a mitotic exit network regulator and binds to Tem1p which is an important component in MEN. Mad2 is a component of a separate spindle checkpoint. Mad2 dependent pathway prevents the metaphase to anaphase transition under spindle damage. Because yeast cells have cell walls, septum formation follows actin ring contraction. Here it was investigated how microtubule defects affect cytokinesis in …

Modulatory Efferent Morphology And Target Allocation With Co-Innervating Leech Motor Neurons, James Melchior

Masters Theses

The leech, Hirudo, has two efferents that converge upon the heart tube (HT) muscle; the segmentally iterated heart excitor motor neurons (HE), and the modulatory heart accessory neurons (HA). Using intracellular dye injections and antibody labeling, we were able to visualize and distinguish the morphologies of these two efferents. We found that HA(5) provides dense coverage of the anterior segments of heart tube muscle and extends along the HT into the posterior segments 6 and 7, while HA(6) projects centrally for several segments before extending into the periphery. HA(5) and HA(6) homologs show little or no overlap on HT …

Spatial And Temporal Distribution Of Total Heterotroph And Fecal Coliform Bacteria In An Urban Stream, Heather R. English

Masters Theses

Total heterotroph bacteria populations in the Sangamon River in east central Illinois, U.S.A. were monitored during a period between June and October in 2003 and 2004. Fecal coliforms (FC) were also monitored during this period in 2004. The four sites monitored were upstream and downstream of the Sanitary District of Decatur (SOD). All areas ofthe river monitored showed similar populations ofboth total heterotrophs and FCs. No correlations could be made between increases in populations of total heterotrophs or FC in relation to flow rate, dissolved oxygen, or total solids. Most sites were found to exceed the recommended amount ofFC for …

Long Term Population Dynamics For Rosa Multiflora In A Successional System, Stephen Eugene Banasiak

Masters Theses

There is a real need for long term studies of invasions to develop a more complete understanding of the factors that influence species spread. Rosa multiflora was chosen to study to address this issue due to its pervasiveness throughout the study site's successional history. The study-site was composed of 10 abandoned agricultural fields near an old growth forest with fifty years of annual vegetation data collection. R. multiflora colonization and extinction rates were examined along with frequency and coefficient of variance since field abandonment. Environmental factors such as rainfall, tree cover, and common species and their effects on R. multiflora …

The Influence Of Extreme Hydrologic Events On Benthic Macroinvertebrate Assemblages In The Sangamon River, Decatur, Illinois, Catherine E. Ciak

Masters Theses

One of the most extreme disturbances to aquatic systems is river impoundment. Dams alter a river's ecological processes by changing the flow of water, nutrients, sediment, biota and energy. Anthropogenic flow variability can lead to changes in natural seasonal variation, which has subsequent effects on biological assemblages within the ecosystem. This study investigated the influence of extreme hydrologic events on benthic macroinvertebrate assemblages within the Sangamon River in Decatur, Illinois. Four sites were sampled from June-August 2006 using the EPA 20 jab method. Samples were processed and macroinvertebrates were identified to family or lowest identifiable taxonomic group. Primer 6.1.6 was …