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Articles 961 - 990 of 1681
Full-Text Articles in Biology
Identification Of 23 Specific Nucleotide Patterns In The Hn Gene Of Newcastle Disease Viruses Isolated From Iran, Majid Esmaelizad, Mehdi P. Ashtiani, Saber Jelokhani Niaraki, Khadije Hashemnejad
Identification Of 23 Specific Nucleotide Patterns In The Hn Gene Of Newcastle Disease Viruses Isolated From Iran, Majid Esmaelizad, Mehdi P. Ashtiani, Saber Jelokhani Niaraki, Khadije Hashemnejad
Turkish Journal of Biology
Newcastle disease is considered the most contagious poultry disease and may cause severe economic loss in the poultry industry. The present study was undertaken to focus on molecular and phylogenetic analyses of the hemagglutinin-neuraminidase (HN) gene in the Iranian isolates. The nucleotide sequence of the HN (780-903 nt) coding region of 6 Newcastle disease viruses (NDVs), isolated from various outbreaks in Iran, was determined and aligned with previously published sequences of 26 NDV isolates in GenBank; the resulting alignment was then analyzed in terms of genetic variation. The number of nucleotide sequence variations presented by each of the isolates indicated …
Genetic Diversity And Identification Of Some Turkish Cotton Genotypes (Gossypium Hirsutum L.) By Rapd-Pcr Analysis, Yonca Surgun, Beki̇r Çöl, Betül Bürün
Genetic Diversity And Identification Of Some Turkish Cotton Genotypes (Gossypium Hirsutum L.) By Rapd-Pcr Analysis, Yonca Surgun, Beki̇r Çöl, Betül Bürün
Turkish Journal of Biology
Cotton (Gossypium hirsutum L.) is the leading fiber crop and the second most important oil seed crop in the world. This study aimed to analyze the genetic differences among 9 cotton genotypes of cultivars from Turkey using the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) method. A total of 42 RAPD primers were used to determine polymorphism among the genotypes and 34 of the primers produced scorable results, with 319 RAPD-PCR bands obtained. The rate of polymorphism was calculated to be 18.1%, and genetic similarity between any 2 genotypes ranged from 90.2% to 96.5%. By evaluating the presence and absence …
Apoplastic And Symplastic Solute Concentrations Contribute To Osmotic Adjustment In Bean Genotypes During Drought Stress, Nesli̇han Saruhan Güler, Aykut Sağlam, Mehmet Demi̇ralay, Asi̇m Kadioğlu
Apoplastic And Symplastic Solute Concentrations Contribute To Osmotic Adjustment In Bean Genotypes During Drought Stress, Nesli̇han Saruhan Güler, Aykut Sağlam, Mehmet Demi̇ralay, Asi̇m Kadioğlu
Turkish Journal of Biology
The present study investigates changes in the inorganic ions, proline, and endogenous abscisic acid (ABA) contents of the apoplastic and symplastic compartments of leaves from drought-tolerant (Yakutiye) and drought-sensitive (Zulbiye) cultivars of the common bean (Phaseolus vulgaris L.). Drought stress caused a decrease in leaf water potential and stomatal conductance in both cultivars. Concentrations of proline in the drought-tolerant and drought-sensitive cultivars increased in response to drought stress in both compartments. The symplastic K^+ concentration decreased in both cultivars. However, the opposite trend was observed concerning K^+ concentrations in the apoplastic areas. While the symplastic Na^+ concentrations significantly decreased in …
Usefulness Of Ca 15-3 For Breast Or Ovarian Primary Sites In Metastatic Adenocarcinoma Of Pleural Fluid, Serpi̇l Oğuztüzün, Murat Kiliç, Ni̇sa Tandogan, Lati̇f Öztürk
Usefulness Of Ca 15-3 For Breast Or Ovarian Primary Sites In Metastatic Adenocarcinoma Of Pleural Fluid, Serpi̇l Oğuztüzün, Murat Kiliç, Ni̇sa Tandogan, Lati̇f Öztürk
Turkish Journal of Biology
This study was conducted to evaluate the diagnostic value of CA 15-3, mammaglobin, c-erbB-2, estrogen receptor (ER), progesterone receptor (PR), CK 20, and CA 125 for detecting metastatic breast and ovarian carcinoma in pleural fluids. The material under study consisted of 26 pleural effusions from invasive breast and ovarian cancer patients from the Hannover Cytopathology Institute. Expressions of these markers were studied using immunocytochemistry. All of the breast cancer cases studied showed a positive reaction with CA 15-3, while only 36% with mammaglobin, 21% with c-erbB-2, 36% with ER, and 7% with PR were positive. The sensitivities of CK 20, …
An Anti-Shigella Dysenteriae Bacteriocin From Pediococcus Pentosaceus Mtcc 5151 Cheese Isolate, Renu Agrawal, Shylaja Dharmesh
An Anti-Shigella Dysenteriae Bacteriocin From Pediococcus Pentosaceus Mtcc 5151 Cheese Isolate, Renu Agrawal, Shylaja Dharmesh
Turkish Journal of Biology
A cheese isolate Pediococcus pentosaceus lactic acid bacterium, which has been deposited at the Microbial Type Culture Collection Centre Chandigarh with the accession number MTCC 5151, was tested for anti-Shigella dysenteriae activity and the bacteriocin was characterized. The protein band was observed with tricine sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) as a single band with a molecular mass of 23 kDa. This is a new and novel bacteriocin that inhibits S. dysenteriae and has not yet been reported from P. pentosaceus. It was purified on a Sephacryl column and the active fraction specific for anti-Shigella dysenteriae with 23 kDa …
Karyology Of The Scorzonera L. (Asteraceae) Taxa From Turkey, Esra Marti̇n, Özlem Çeti̇n, Serdar Makbul, Ahmet Duran, Meryem Şeker, Derya Boduroğlu, Bahri̇ye Eşmekaya
Karyology Of The Scorzonera L. (Asteraceae) Taxa From Turkey, Esra Marti̇n, Özlem Çeti̇n, Serdar Makbul, Ahmet Duran, Meryem Şeker, Derya Boduroğlu, Bahri̇ye Eşmekaya
Turkish Journal of Biology
In this study, the karyotypes of 13 taxa of the tribe Lactuceae growing naturally in Turkey were examined. Of the species of the genus Scorzonera L. (Asteraceae), S. laciniata L. subsp. laciniata, S. cana (C.A.Mey.) Hoffm. var. jacquiniana (W.Koch) Chamb., S. suberosa C.Koch subsp. suberosa, S. mollis M.Bieb. subsp. mollis, S. papposa DC., S. lacera Boiss. & Bal., S. elata Boiss., and S. parviflora Jacq. have a diploid chromosome number of 2n = 14. For S. phaeopappa (Boiss.) Boiss., this number is 2n = 28, and for S. eriophora DC., S. pseudolanata Grossh., S. tomentosa L., and S. kotschyi Boiss., …
Hepatoprotective Effect Of Vitamins C And E Against Gasoline Vapor-Induced Liver Injury In Male Rats, Friday Effiong Uboh, Patrick Ekong Ebong, Henry Dan Akpan, Itoro Friday Usoh
Hepatoprotective Effect Of Vitamins C And E Against Gasoline Vapor-Induced Liver Injury In Male Rats, Friday Effiong Uboh, Patrick Ekong Ebong, Henry Dan Akpan, Itoro Friday Usoh
Turkish Journal of Biology
The protective effect of vitamins C and E against gasoline vapor-induced liver injury was investigated in rats. Liver injury was assessed from the activities of liver function diagnostic indices including serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma glutamyl transferase (\gamma-GT), alkaline phosphatase (ALP) activities, total serum protein (TSP), albumin concentrations, and the histological architectures of the liver tissues of the experimental animals. The results showed that gasoline vapors caused a significant (P = 0.05) higher than that of vitamin C. The results of our study suggest a protective effect of vitamin C and vitamin E against gasoline vapor-induced liver …
Accumulation Of Poly (3-Hydroxybutyrate) By Microbacterium Barkeri Dsm 20145, Srividya Shivakumar
Accumulation Of Poly (3-Hydroxybutyrate) By Microbacterium Barkeri Dsm 20145, Srividya Shivakumar
Turkish Journal of Biology
A gram-positive bacterium that accumulated poly(3-hydroxybutyric) acid (PHB) was isolated from petrol bunk soil. From its morphological and physiological properties and the nucleotide sequence (about 1.5 kb) of its 16S rDNA, it was identified as Microbacterium barkeri DSM 20145. Based on the available literature, this is the first report of PHB accumulation by Microbacterium sp. The isolate accumulated 0.6 g/L, or 29.7%, of PHB under growth conditions in a nitrogen-deficient medium containing glucose and yeast extract (4:1 ratio), with maximum production by 48 h. The capacity of the isolate to produce PHB under different carbon and nitrogen sources was also …
Fatty Acid Composition Of The Seeds Of Some Species Of Peucedanum (Kral Otu) From Turkey, Nükhet Akpinar, Sali̇h Görgün, Hüseyi̇n Aşkin Akpulat, Mehmet Ali̇ Akpinar, Apdurahman Aktümsek, Ali̇ Emre Akpinar
Fatty Acid Composition Of The Seeds Of Some Species Of Peucedanum (Kral Otu) From Turkey, Nükhet Akpinar, Sali̇h Görgün, Hüseyi̇n Aşkin Akpulat, Mehmet Ali̇ Akpinar, Apdurahman Aktümsek, Ali̇ Emre Akpinar
Turkish Journal of Biology
The fatty acid compositions of the seeds of Peucedanum ruthenicum, Peucedanum chryseum, Peucedanum palimbioides, Peucedanum obtusifolium, and Peucedanum zedelmeierianum were investigated by gas chromatography (GC). The main fatty acids in the species were found to be palmitic acid (C 16:0, 5.78%-14.68%), oleic acid (C 18:1 \omega-9, 31.28%-68.06%), and linoleic acid (C 18:2 \omega-6, 15.99%-33.74%). Trace amounts of cis-vaccenic acid (C 18:1 \omega-7, 0.82%-1.40%) were found in the seed oils of all of the species investigated.
Antimicrobial And Antiviral Effects Of Essential Oils From Selected Umbelliferae And Labiatae Plants And Individual Essential Oil Components, İlkay Erdoğan Orhan, Berri̇n Özçeli̇k, Murat Kartal, Yüksel Kan
Antimicrobial And Antiviral Effects Of Essential Oils From Selected Umbelliferae And Labiatae Plants And Individual Essential Oil Components, İlkay Erdoğan Orhan, Berri̇n Özçeli̇k, Murat Kartal, Yüksel Kan
Turkish Journal of Biology
The essential oils obtained from Anethum graveolens, Foeniculum vulgare collected at fully-mature and flowering stages, Mentha piperita, Mentha spicata, Lavandula officinalis, Ocimum basilicum (green- and purple-leaf varieties), Origanum onites, O. vulgare, O. munitiflorum, O. majorana, Rosmarinus officinalis, Salvia officinalis, and Satureja cuneifolia, as well as the widely encountered components in essential oils (\gamma-terpinene, 4-allylanisole, (-)-carvone, dihydrocarvone, D-limonene, (-)-phencone, cuminyl alcohol, cuminyl aldehyde, cuminol, trans-anethole, camphene, isoborneol, (-)-borneol, L-bornyl acetate, 2-decanol, 2-heptanol, methylheptane, farnesol, nerol, isopulegol, citral, citronellal, citronellol, geraniol, geranyl ester, linalool, linalyl oxide, linalyl ester, \alpha-pinene, \beta-pinene, piperitone, (-)-menthol, isomenthone, carvacrol, thymol, vanillin, and eugenol), were screened for their …
Morphological And 18s Rrna Analysis Of Coccoid Green Algae Isolated From Lakes Of Kızılırmak Delta, Eli̇f Neyran Soylu, Ari̇f Gönülol
Morphological And 18s Rrna Analysis Of Coccoid Green Algae Isolated From Lakes Of Kızılırmak Delta, Eli̇f Neyran Soylu, Ari̇f Gönülol
Turkish Journal of Biology
Molecular techniques are used today in many areas and have increased the confidence level of studies. The aim of the study was to compare the classical morphological characters of coccoid green algae under field and culture conditions by using modern molecular analysis. To achieve this aim, we first isolated the coccoid green algae from Liman and Cernek lakes. The morphological variations of green algae were observed under culture conditions and the phylogenetic relationships of these strains were defined according to the 18S rRNA gene sequences. According to sequence analysis, 2 of the isolated species had high similarity to Scenedesmus subspicatus …
Genetic Variability Of Beauveria Bassiana And Metarhizium Anisopliae Var. Anisopliae Isolates Obtained From The Eastern Black Sea Region Of Turkey, Ali̇ Sevi̇m, Monica Höfte, Zi̇hni̇ Demi̇rbağ
Genetic Variability Of Beauveria Bassiana And Metarhizium Anisopliae Var. Anisopliae Isolates Obtained From The Eastern Black Sea Region Of Turkey, Ali̇ Sevi̇m, Monica Höfte, Zi̇hni̇ Demi̇rbağ
Turkish Journal of Biology
Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae var. anisopliae (Metschnikoff) Sorokin are the most common entomopathogenic fungi used in microbial control programs all over the world. Assessments of the genetic variability of these 2 important species are useful for the development of effective biocontrol strategies and for evaluating the impact of artificial epizootics. In this study, the genetic diversity of 13 B. bassiana and 33 M. anisopliae var. anisopliae strains isolated from the hazelnut-growing region of Turkey was determined by using the amplified fragment length polymorphism (AFLP) and \alpha- and \beta-isoenzyme analyses. Cluster analysis of AFLP data clearly separated both …
Cytotoxic Action Of Lead Nitrate On Cytomorphology Of Trigonella Foenum-Graecum L., Sana Choudhary, Mohammad Yunus Khalil Ansari, Zeba Khan, Honey Gupta
Cytotoxic Action Of Lead Nitrate On Cytomorphology Of Trigonella Foenum-Graecum L., Sana Choudhary, Mohammad Yunus Khalil Ansari, Zeba Khan, Honey Gupta
Turkish Journal of Biology
The present experiment was undertaken to determine the mutagenic potential of lead on the cytomorphology of Trigonella foenum-graecum L. and to determine the maximum concentration of lead nitrate that induces maximum genetic variability. The seeds were treated with 6 different concentrations (10, 25, 50, 100, 200, and 400 ppm) of lead nitrate. The higher concentrations of lead nitrate significantly reduced plant height, number of branches per plant, pollen fertility, and yield; higher concentrations also caused variation in the plants at the seedling and mature stages. In addition, concentrations affected the pairing of homologous chromosomes and spindle formation. This resulted in …
Bioactive Compounds From Discarded Mushroom Beds, Svsssl Hima Bindu Nidadavolu, Ram Prasad Metuku, Samatha Burra, Rajitha Byram, Singara Charya Maringanti
Bioactive Compounds From Discarded Mushroom Beds, Svsssl Hima Bindu Nidadavolu, Ram Prasad Metuku, Samatha Burra, Rajitha Byram, Singara Charya Maringanti
Turkish Journal of Biology
Discarded dried button, dried oyster, and fresh button mushroom beds were examined for their bioactive compounds and industrial enzymes. Levels of reducing sugars and total sugars were high in all mushroom beds. Carboxymethyl cellulase activity was higher (920 µg/mL) in the dried oyster mushroom bed extract. Schizophyllum commune, Fomitopsis feei, Trametes gibbosa, and Trametes elegans were grown on direct mushroom bed extract, 0.2% glucose-containing mushroom bed extract, and common production medium for determination of growth and lignolytic activities. Since lignolytic activities were high with the direct mushroom bed extract, this medium was further diluted to 1:1 and 1:2 ratios and …
A Preliminary Attempt For Efficient Genetic Transformation And Regeneration Of Legume Mucuna Pruriens L. Mediated By Agrobacterium Tumefaciens, Raghavendra Sathyanarayana, Vadlapudi Kumar, Chapeyl.Kumaran Ramesh, Mahadevappa Paresha, Mahaboob Habeebulla Moinuddin Khan
A Preliminary Attempt For Efficient Genetic Transformation And Regeneration Of Legume Mucuna Pruriens L. Mediated By Agrobacterium Tumefaciens, Raghavendra Sathyanarayana, Vadlapudi Kumar, Chapeyl.Kumaran Ramesh, Mahadevappa Paresha, Mahaboob Habeebulla Moinuddin Khan
Turkish Journal of Biology
Mucuna pruriens belongs to the family Papillonaceae. The seeds and leaves of these plants contain a large amount of L-DOPA. L-DOPA (L-3,4-dihydroxyphenylalanine) is one of the most widely used drugs in the treatment of Parkinson disease. Development of an efficient gene transfer method is an absolute requirement for genetically improving Mucuna pruriens and creating plants with more desirable traits. A simple protocol was developed for the Agrobacterium-mediated stable genetic transformation of M. pruriens. Agrobacterium tumefaciens strain EHA 101, containing the vector pCAMBIA1305 and the hptII and GUS plus genes, was used for the gene transfer experiments. Putative transgenic shoots were …
Determination Of Genetic Relationships Among Velezia L. Caryophyllaceae) Species Using Rapd Markers, İlham Eröz Poyraz, Emel Sözen, Ebru Ataşlar, İsmai̇l Poyraz
Determination Of Genetic Relationships Among Velezia L. Caryophyllaceae) Species Using Rapd Markers, İlham Eröz Poyraz, Emel Sözen, Ebru Ataşlar, İsmai̇l Poyraz
Turkish Journal of Biology
Random amplified polymorphic DNA (RAPD) markers were used to determine genetic relationships among Velezia L. species from Turkey. A total of 432 amplified bands were obtained using 14 RAPD primers. The polymorphism in RAPD markers was high (98.61%) and was sufficient to distinguish each species. The degree of band-sharing was used for evaluating the genetic similarity between species and for constructing a dendrogram by the unweighted pair group method with arithmetic mean (UPGMA). Genetic relationships among the species were found to be fully consistent with those obtained by the use of morphological characters. Data obtained from our study demonstrated that …
Poly-3-Hydroxybutyrate-Producing Extreme Halophilic Archaeon: Haloferax Sp. Ma10 Isolated From Çamaltı Saltern, İzmir, Ebru Teki̇n, Mustafa Ateş, Özge Kahraman
Poly-3-Hydroxybutyrate-Producing Extreme Halophilic Archaeon: Haloferax Sp. Ma10 Isolated From Çamaltı Saltern, İzmir, Ebru Teki̇n, Mustafa Ateş, Özge Kahraman
Turkish Journal of Biology
İzmir Çamaltı Saltern is the biggest seawater-based saltern in Turkey. To date, it has not been investigated extensively for the existence of poly-3-hydroxybutyrate (PHB)-producing species. In this study, 14 extremely halophilic archaea were isolated, purified, and screened for PHB production. One strain was then selected as the best PHB producer, further cultivated in different PHB media, and compared with a positive control, Haloferax mediterranei ATCC 33500. PHB was extracted from cells and measured with a spectrophotometer, and then the amount of PHB was measured through comparison with standard PHB. The detected high PHB yield was 6.53% of the dry cell …
Biofilm Formation Comparison Of The Sanipacking® Cooling Tower Fill Material Against Standard Polypropylene Fill Material In A Recirculating Model Water System, İrfan Türetgen, Nazmi̇ye Özlem Şanli Yürüdü, Imke Norden
Biofilm Formation Comparison Of The Sanipacking® Cooling Tower Fill Material Against Standard Polypropylene Fill Material In A Recirculating Model Water System, İrfan Türetgen, Nazmi̇ye Özlem Şanli Yürüdü, Imke Norden
Turkish Journal of Biology
Cooling towers are heat rejection systems that are used in some industrial applications, and they have the potential to develop infectious concentrations of Legionella pneumophila. SANIPACKING® cooling tower fill material and standard polypropylene fill material were compared in terms of biofilm formation potential and anti-Legionella activity within a 4-month period using a laboratory-scale recirculating water system. The recirculating water system was experimentally infected with a L. pneumophila standard strain (ATCC 33152) suspension and operated continuously until all experiments had been completed. Results showed that the L. pneumophila rapidly multiplied in a short time during the study within the model system …
Genetic Diversity Among Salvia Miltiorrhiza Bunge And Related Species Inferred From Nrdna Its Sequences, Li Zhang, Hong-Xia Zhao, Xing Fan, Meng Wang, Chunbang Ding, Rui-Wu Yang, Zhong-Qiong Yin, Xian-Li Xie, Yong-Hong Zhou, De-Guang Wan
Genetic Diversity Among Salvia Miltiorrhiza Bunge And Related Species Inferred From Nrdna Its Sequences, Li Zhang, Hong-Xia Zhao, Xing Fan, Meng Wang, Chunbang Ding, Rui-Wu Yang, Zhong-Qiong Yin, Xian-Li Xie, Yong-Hong Zhou, De-Guang Wan
Turkish Journal of Biology
To investigate the genetic diversity and phylogenetic relationships of Salvia miltiorrhiza and related species, we analyzed the nuclear ribosomal DNA internal transcribed spacer (ITS) region for 7 accessions of Salvia miltiorrhiza and another 23 samples from other taxa within the genus Salvia by maximum parsimony and Bayesian inference analyses. There were 257 variation sites amounting to 40.8% of the total base pairs. All of the data revealed abundant genetic diversity in the genus Salvia. The results showed that the tested materials could be grouped into 3 clusters, and the 3 different occasions in Salvia were clustered into 1 clade, which …
The Effect Of Salt Stress On Antioxidative Enzymes And Proline Content Of Two Turkish Tobacco Varieties, Özge Çeli̇k, Çi̇men Atak
The Effect Of Salt Stress On Antioxidative Enzymes And Proline Content Of Two Turkish Tobacco Varieties, Özge Çeli̇k, Çi̇men Atak
Turkish Journal of Biology
The aim of this study was to compare the salinity tolerances of 2 oriental tobacco varieties (İzmir Özbaş and Akhisar 97). Salinity stress experiments were performed under both in vitro and in vivo conditions. Seedlings of each variety were subjected to 0, 50, 100, 150, 200, 250, 300, and 350 mM NaCl. Photosynthetic pigment levels, lipid peroxidation rate, total protein content, antioxidant enzyme activities, and proline concentrations were determined for seedlings treated with salt for 14 days. The Akhisar 97 variety was found to be more sensitive to salinity stress than the İzmir Özbaş variety. Although proline is thought to …
Influence Of Phytophthora Capsici L. Inoculation On Disease Severity, Necrosis Length, Peroxidase And Catalase Activity, And Phenolic Content Of Resistant And Susceptible Pepper (Capsicum Annuum L.) Plants, Esra Koç, Ayşen Sülün Üstün
Influence Of Phytophthora Capsici L. Inoculation On Disease Severity, Necrosis Length, Peroxidase And Catalase Activity, And Phenolic Content Of Resistant And Susceptible Pepper (Capsicum Annuum L.) Plants, Esra Koç, Ayşen Sülün Üstün
Turkish Journal of Biology
This study explored the level of infection caused by different inoculum concentrations (10^2, 10^3, and 10^4 zoospores mL^-^1) of Phytophthora capsici in 3 pepper cultivars at days 2, 4, and 6. The effect that the infection induced on the peroxidase (POX), catalase (CAT), and phenolics of resistant and sensitive seedlings, as well as the defense mechanism against the pathogen, were also investigated. The resistance of PM-702 against the isolate used was high, whereas KM-Hot and DEM-8 displayed sensitive reactions. As a result of analyses of the leaves and stems from the 3 pepper cultivars, important changes in biochemical parameters were …
Deletion Of Polyphosphate Kinase Gene (Ppk)Has A Stimulatory Effect On Actinorhodin Production By Streptomyces Coelicolor A3(2), İrem Yalim Camci, Tuğrul Doruk, Ümmehan Avi̇can, Sedef Tunca Gedi̇k
Deletion Of Polyphosphate Kinase Gene (Ppk)Has A Stimulatory Effect On Actinorhodin Production By Streptomyces Coelicolor A3(2), İrem Yalim Camci, Tuğrul Doruk, Ümmehan Avi̇can, Sedef Tunca Gedi̇k
Turkish Journal of Biology
Polyphosphate, which is synthesized by the enzyme polyP kinase (PPK), is an important energy and phosphate storage polymer that plays a crucial role in the regulation of adaptive responses of cells to physical and chemical stresses. In this work, the polyphosphate kinase gene (ppk) of Streptomyces coelicolor was deleted, and the effect of this mutation on actinorhodin and undecylprodigiosin biosynthesis was investigated. Deletion of the ppk gene had a stimulatory effect on actinorhodin production; the mutant strain produced about 5 times more antibiotic compared to the wild type strain at 120 h of growth. There was no difference in undecylprodigiosin …
Calcium Oxalate Crystal Types In Three Oak Species (Quercus L.) In Turkey, Bedri̇ Serdar, Hati̇ce Demi̇ray
Calcium Oxalate Crystal Types In Three Oak Species (Quercus L.) In Turkey, Bedri̇ Serdar, Hati̇ce Demi̇ray
Turkish Journal of Biology
Crystal types from 3 oak species representing 3 different sections of the genus Quercus were identified. Crystals were studied with scanning electron microscopy after localisation by light microscopy. The crystals were composed of calcium oxalate silicates as whewellite (calcium oxalate monohydrate) composites. In Quercus macranthera Fisch. et Mey. subsp. syspirensis (C.Koch) Menitsky (İspir oak) from the white oaks (section Quercus: Leucobalanus), whewellite and trihydrated weddellites coexisted. Axial parenchyma cells included 30 crystal chains with walls as chambers in uniseriate ray cells; some cells had many crystals without any chambers and were small. Long thin crystals were found adherent to the …
Optimization Of The Process Parameters For The Utilization Of Orange Peel To Produce Polygalacturonase By Solid-State Fermentation From An Aspergillus Sojae Mutant Strain, Hande Demi̇r, Ni̇han Göğüş, Canan Tari, Doreen Heerd, Marcelo Fernandez Lahore
Optimization Of The Process Parameters For The Utilization Of Orange Peel To Produce Polygalacturonase By Solid-State Fermentation From An Aspergillus Sojae Mutant Strain, Hande Demi̇r, Ni̇han Göğüş, Canan Tari, Doreen Heerd, Marcelo Fernandez Lahore
Turkish Journal of Biology
The effect of orange peel concentration, HCl concentration, incubation time and temperature, and inoculum size on the spore count and activity of polygalacturonase (PG) enzyme produced from Aspergillus sojae M3 by solid-state fermentation was screened using 2^k factorial design. Orange peel and HCl concentrations and incubation time were significant factors affecting the responses. Optimum conditions favoring both PG and spore production from Aspergillus sojae M3 were determined as 2% orange peel and 50 mM HCl concentrations at 22 °C and 4.3 days of incubation. An overlay plot was constructed for use as a practical chart for production of high enzyme …
Induction Of Salt-Tolerant Potato (Solanum Tuberosum L.) Mutants With Gamma Irradiation And Characterization Of Genetic Variations Via Rapd-Pcr Analysis, Orkun Yaycili, Sema Ali̇kamanoğlu
Induction Of Salt-Tolerant Potato (Solanum Tuberosum L.) Mutants With Gamma Irradiation And Characterization Of Genetic Variations Via Rapd-Pcr Analysis, Orkun Yaycili, Sema Ali̇kamanoğlu
Turkish Journal of Biology
Salt-tolerant mutants of potato (Solanum tuberosum L. 'Marfona') were obtained via gamma irradiation. Node explants of the Marfona potato plant were treated with various dosages of gamma irradiation, and the M_1V_2 and M_1V_3 clonal generations were developed. Selection of salt-tolerant mutants was accomplished by in vitro selection media containing 50, 100, and 125 mM NaCl. Molecular-level differences between the control and mutant plants were elucidated using RAPD-PCR method, and the polymorphism rate according to the selected primers was calculated as 89.66%. Genetic distances between the controls and mutants were also calculated, and related dendrograms were produced. On average the mutants …
Genetic Diversity Of Natural Cyclamen Alpinum Populations, Belgi̇n Göçmen Taşkin, Ni̇lden Vardareli̇, Ersi̇n Doğaç, Ramazan Mammadov, Vatan Taşkin
Genetic Diversity Of Natural Cyclamen Alpinum Populations, Belgi̇n Göçmen Taşkin, Ni̇lden Vardareli̇, Ersi̇n Doğaç, Ramazan Mammadov, Vatan Taşkin
Turkish Journal of Biology
The focus of this study was Cyclamen alpinum (formerly C. trochopteranthum). Habitat fragmentation, environmental degradation, and overharvesting of tubers have exerted pressure on native populations of this valuable ornamental species. Although the entire Cyclamen genus is in the Convention on International Trade in Endangered Species of Wild Fauna and Flora-Appendix II (CITES II), no species has yet been red-listed. Estimating the level and distribution of genetic variation in populations of rare and endemic species is important for conserving genetic diversity within a species in the context of well-developed conservation strategies. Currently, DNA markers are the most effective means used to …
Alkaline Protease Production Of Bacillus Cohnii Apt5, Ni̇lgün Teki̇n, Arzu Çöleri̇ Ci̇han, Zeki̇ye Serpi̇l Takaç, Canan Yağci Tüzün, Kenan Tunç, Cumhur Çökmüş
Alkaline Protease Production Of Bacillus Cohnii Apt5, Ni̇lgün Teki̇n, Arzu Çöleri̇ Ci̇han, Zeki̇ye Serpi̇l Takaç, Canan Yağci Tüzün, Kenan Tunç, Cumhur Çökmüş
Turkish Journal of Biology
An obligate alkaliphilic Bacillus strain was isolated from a soil sample and identified as Bacillus cohnii APT5 based upon phylogenetic and phenotypic analyses. The optimum growth pH of B. cohnii APT5 was 10.0. B. cohnii APT5 was also found capable of producing an extracellular alkaline protease that showed optimum activity (693,318 U/min) at 50 °C and pH 11.0 when grown in a medium containing casein. The enzyme was partially purified 3.22-fold with a yield of 78.74% after acetone precipitation and cation exchange column chromatography, respectively. The partially purified enzyme maintained its activity when incubated at 50 °C for 2 h. …
Genotoxic And Mutagenic Effects Of Aqueous Extract From Aerial Parts Of Achillea Teretifolia, Di̇lek Akyil, Sedat Oktay, Recep Li̇man, Yasi̇n Eren, Muhsi̇n Konuk
Genotoxic And Mutagenic Effects Of Aqueous Extract From Aerial Parts Of Achillea Teretifolia, Di̇lek Akyil, Sedat Oktay, Recep Li̇man, Yasi̇n Eren, Muhsi̇n Konuk
Turkish Journal of Biology
Genotoxic and mutagenic effects of aqueous extract from aerial parts of Achillea teretifolia (At-ext) were investigated using both Allium cepa root meristematic cells and a short-term mutation assay in Salmonella typhimurium with TA98 and TA100 strains in the presence or absence of S9 mix, respectively. In the Allium tests, EC_{50} value was determined as 50 g/L, and 0.5 × EC_{50}, EC_{50}, and 2 × EC_{50} concentrations of At-ext were applied to onion tuber roots. Methyl methane sulfonate (MMS, 10 ppm) and distilled water were used as positive and negative controls, respectively. The mitotic index (MI) increased in all concentrations in …
Investigation Of Genetic Variation In Tunisian Date Palm (Phoenix Dactylifera L.) Cultivars Using Issr Marker Systems And Their Relation With Fruit Characteristics, Hammadi Hamza, Mohamed Ali Benabderrahim, Mokhtar Elbekkay, Guasmi Ferdaous, Tebra Triki, Ali Ferchichi
Investigation Of Genetic Variation In Tunisian Date Palm (Phoenix Dactylifera L.) Cultivars Using Issr Marker Systems And Their Relation With Fruit Characteristics, Hammadi Hamza, Mohamed Ali Benabderrahim, Mokhtar Elbekkay, Guasmi Ferdaous, Tebra Triki, Ali Ferchichi
Turkish Journal of Biology
Phoenix dactylifera L. was introduced in Tunisia a very long time ago and plays an important socioeconomic role, especially in the south of the country. Genetic diversity and relationships among 26 cultivars were assessed with 7 ISSR primers. A total of 43 amplified bands were obtained. Principal component analyses based on Nei genetic distances showed no geographical separation with the exception of the Dhahbi cultivar, which has a limited geographical distribution. A group of cultivars that are also phonetically clustered was distinguished. These cultivars have a common maturity period and a common fruit consistency. The Mantel test emphasizes a significant …
Antioxidant Enzymes And Gst Activity In Natural Populations Of Holandriana Holandrii From The Bosna River, Jelena Vrankovic, Svetlana Labus-Blagojevic, Bela Csanyi, Jarmila Makovinska, Olga Cvetkovic, Zoran Gacic, Dusko Blagojevic, Momir Paunovic
Antioxidant Enzymes And Gst Activity In Natural Populations Of Holandriana Holandrii From The Bosna River, Jelena Vrankovic, Svetlana Labus-Blagojevic, Bela Csanyi, Jarmila Makovinska, Olga Cvetkovic, Zoran Gacic, Dusko Blagojevic, Momir Paunovic
Turkish Journal of Biology
Specimens of the freshwater snail Holandriana holandrii affected by different levels of contamination were collected from 3 sites within the Bosna River Basin, i.e. Visoko, Doboj, and Modrica. The activities of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR), and glutathione-S-transferase (GST) were investigated in order to understand their variation with respect to the pollution status of the sampling locations. The results revealed a significant increase in CAT, GPx, and GST activities in the snails collected from Modrica, suggesting that the animals at this location are exposed to a higher level of oxidative …