Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- Apoptosis (44)
- Antimicrobial activity (32)
- Cancer (32)
- Oxidative stress (31)
- Cytotoxicity (30)
-
- Antioxidant (25)
- COVID-19 (25)
- Breast cancer (24)
- SARS-CoV-2 (24)
- Melatonin (23)
- Antioxidant activity (22)
- Purification (20)
- Mesenchymal stem cells (19)
- Turkey (18)
- Antioxidant enzymes (17)
- Rat (17)
- Antibacterial activity (15)
- Inflammation (15)
- Gene expression (14)
- Regeneration (14)
- Bioinformatics (13)
- Characterization (13)
- Liver (13)
- Antibacterial (12)
- Genetic diversity (12)
- Growth (12)
- Saccharomyces cerevisiae (11)
- Streptomyces (11)
- Toxicity (11)
- Catalase (10)
- Publication Year
Articles 931 - 960 of 1681
Full-Text Articles in Biology
Bioaccumulation Of Tributyltin And Its Impact On Spermatogenesis In Mud Crab Scylla Serrata (Forskal), Revathi Peranandam, Iyapparaj Palanisamy, Munuswamy Natesan, Arockia Vasanthi Lourduraj, Krishnan Muthukalingan
Bioaccumulation Of Tributyltin And Its Impact On Spermatogenesis In Mud Crab Scylla Serrata (Forskal), Revathi Peranandam, Iyapparaj Palanisamy, Munuswamy Natesan, Arockia Vasanthi Lourduraj, Krishnan Muthukalingan
Turkish Journal of Biology
The effects of tributyltin (TBT) on the spermatogenesis of mud crab Scylla serrata were studied. TBT-exposed crabs exhibited a decrease in the gonadosomatic Index and the hepatosomatic Index compared to the crabs in the control group. Bioaccumulation of the TBT levels also varied in different tissues. The treated crabs showed variation in the weight of the testes with respect to TBT concentrations. Histological results clearly indicated that the control crabs showed spermatophores and granular substances were seen filling up the lumen of the seminiferous tubules. The testes of the experimental crabs illustrated that disruption in the seminiferous tubule membrane architecture, …
L-Arginine Protects Against Ethylene Glycol-Induced Gastric Mucosal Damage In Rats: Immunohistochemical And Electron Microscopic Study, Samah Kandeel, Thoryaa El_Deeb, Ehsan Salah, Nafisa El-Bakary, Naglaa Sarhan, Mohamed Balaha, Hiroki Sakai, Tokuma Yanai
L-Arginine Protects Against Ethylene Glycol-Induced Gastric Mucosal Damage In Rats: Immunohistochemical And Electron Microscopic Study, Samah Kandeel, Thoryaa El_Deeb, Ehsan Salah, Nafisa El-Bakary, Naglaa Sarhan, Mohamed Balaha, Hiroki Sakai, Tokuma Yanai
Turkish Journal of Biology
Ethylene glycol is an alcohol widely used in industry as an antifreeze. It affects the normal gastric mucosal defense mechanisms, inducing surface ulcerations. L-arginine is a precursor of nitric oxide that exhibits multiple biological properties; among them is the acceleration of wound healing, especially in the stomach. Caspase-3 immunohistochemistry, and semithin and ultrathin section examinations were used to investigate the possible protective effect of L-arginine on ethylene glycol-induced gastric mucosal damage in rats. Three animal groups were used: group A (control group), group B (received 3.3 mL/kg ethylene glycol for 2 weeks), and group C (received 200 mg/kg of L-arginine …
Morphological And Molecular Identification Of Pennate Diatoms Isolated From Urla, İzmir, Coast Of The Aegean Sea, Kutsal Kesi̇ci̇, İnci̇ Tüney, Doğuş Zeren, Mustafa Güden, Atakan Sukatar
Morphological And Molecular Identification Of Pennate Diatoms Isolated From Urla, İzmir, Coast Of The Aegean Sea, Kutsal Kesi̇ci̇, İnci̇ Tüney, Doğuş Zeren, Mustafa Güden, Atakan Sukatar
Turkish Journal of Biology
Diatoms represent an important class of aquatic phototrophs. They are not only one of the major contributors to global carbon fixation, but they also play a key role in the biogeochemical cycling of silica. Molecular identification methods based on conserved DNA sequences, such as internal transcribed spacer (ITS) have revolutionized our knowledge and understanding of conventional taxonomy. In this study, we aimed to compare the conventional identification methods with molecular identification methods. To do so, we isolated 4 diatom samples from the coast at Urla and characterized them using light microscopy (LM) and scanning electron microscopy (SEM) according to morphological …
Activation Of The Jak/Stat Pathway In Human Neutrophils By Ndma, Wioletta Ratajczak Wrona, Ewa Jablonska, Marzena Garley, Jakub Jablonski, Piotr Radziwon, Agnieszka Iwaniuk
Activation Of The Jak/Stat Pathway In Human Neutrophils By Ndma, Wioletta Ratajczak Wrona, Ewa Jablonska, Marzena Garley, Jakub Jablonski, Piotr Radziwon, Agnieszka Iwaniuk
Turkish Journal of Biology
The study objective was to assess the role of the JAK/STAT pathway in the induction of nitric oxide (NO) synthesis with the involvement of inducible nitric oxide synthase (iNOS) and in the generation of superoxide anion radical by human neutrophils (PMNs) exposed to N-nitrosodimethylamine (NDMA). Isolated PMNs were incubated in the presence of NDMA. The expression of the study proteins in the cytoplasmic and nuclear fraction was determined by western blotting. Total NO was assayed in cell cultures by Griess reaction. The ability of PMNs to generate superoxide anion radical was assessed using the cytochrome-c reduction test. The current results …
Biopotential Of Verbesina Encelioides (Stem And Leaf Powders) In Silver Nanoparticle Fabrication, Himakshi Bhati-Kushwaha, Chander Parkash Malik
Biopotential Of Verbesina Encelioides (Stem And Leaf Powders) In Silver Nanoparticle Fabrication, Himakshi Bhati-Kushwaha, Chander Parkash Malik
Turkish Journal of Biology
Nanotechnology has opened new opportunities in the field of medical sciences and pharmaceuticals. Commercially, nanoparticles are in great demand in electronics, catalysis, chemistry, energy, and medicine. Today in nanoparticle production green synthesis, using biological material including medicinal plants as a starting material, is in vogue. In the present investigation the powder extract (stems and leaves) of an important medicinal plant, Verbesina encelioides, was used for the biosynthesis of bionanoparticles. The synthesized nanoparticles were screened and characterized by UV-visible spectrophotometer, scanning electron microscopy, X-ray diffraction, and FTIR analyses. The particles were then subjected to antimicrobial assay. Comparative analyses of the antimicrobial …
Membrane And Dna Damaging/Protective Effects Of Eugenol, Eucalyptol, Terpinen-4-Ol, And Camphor At Various Concentrations On Parental And Drug-Resistant H1299 Cells, Aysun Özkan, Ayşe Erdoğan
Membrane And Dna Damaging/Protective Effects Of Eugenol, Eucalyptol, Terpinen-4-Ol, And Camphor At Various Concentrations On Parental And Drug-Resistant H1299 Cells, Aysun Özkan, Ayşe Erdoğan
Turkish Journal of Biology
Eugenol, eucalyptol, terpinen-4-ol, and camphor, used as flavor agents in cosmetic and food products, have both prooxidant and antioxidant activities. To clarify the mechanisms of their cytotoxic effect and the factors affecting their antioxidant/prooxidant activities, we investigated cell membrane and DNA damage/protective effects induced by eugenol, eucalyptol, terpinen-4-ol, and camphor in parental and drug-resistant human lung cancer cell lines. The parental cells were found approximately 3, 6, and 8 times more sensitive to camphor cytotoxicity than drug-resistant cells at 24, 48, and 72 h respectively. The cytotoxic activity for both cell types was found to be in the order of …
Proteomic Evidence For The Plasticity Of Cultured Vascular Smooth Muscle Cells, Ahmet Tarik Baykal, Betül Baykal, Müge Serhatli, Zelal Adigüzel, Mehmet Altuğ Tunçer, Ömer Kaçar, Kemal Baysal, Ceyda Açilan Ayhan
Proteomic Evidence For The Plasticity Of Cultured Vascular Smooth Muscle Cells, Ahmet Tarik Baykal, Betül Baykal, Müge Serhatli, Zelal Adigüzel, Mehmet Altuğ Tunçer, Ömer Kaçar, Kemal Baysal, Ceyda Açilan Ayhan
Turkish Journal of Biology
Morphological, functional, and gene expression studies have established the phenotypic plasticity of smooth muscle cells (SMCs). These cells have been shown to respond to environmental stimulants such as extracellular matrix and growth factors. Cellular changes can vary between extremes defined as the contractile and synthetic states. Various growth factors have been shown to have profound effects on the phenotype of these cells. In this study, we intended to investigate the effects of growth factor-rich medium on the protein expression of vascular SMCs in culture. Interestingly, transiently changing the type of medium did not result in any apparent morphological differences, yet …
Transcriptional Profiling Of Transferrin Gene From Egyptian Cotton Leaf Worm, Spodoptera Littoralis, Nurper Güz, Asli Dağeri̇, Tuğba Erdoğan, Mouzhgan Mousavi, Şeri̇fe Bayram, Mehmet Oktay Gürkan
Transcriptional Profiling Of Transferrin Gene From Egyptian Cotton Leaf Worm, Spodoptera Littoralis, Nurper Güz, Asli Dağeri̇, Tuğba Erdoğan, Mouzhgan Mousavi, Şeri̇fe Bayram, Mehmet Oktay Gürkan
Turkish Journal of Biology
Iron is an essential nutrient for almost all organisms, but it is also a potent toxin because it can catalyze oxidative reactions that are destructive to cells. Transferrin is an essential protein involved in iron metabolism, immunity, and vitellogenesis in insects. We have characterized a cDNA encoding a putative iron-binding transferrin (SpliTrf) in Egyptian cotton leaf worm, Spodoptera littoralis (Lepidoptera: Noctuidae), an important pest that causes extensive losses in many vegetable, fodder, and fiber crops. The structure of the putative SpliTrf showed significant homology to other insect transferrins. Gene expression of SpliTrf was examined from different adult tissues including the …
Genetic Diversity Of Golden Root (Rhodiola Rosea L.) In Northern Norway Based On Recently Developed Ssr Markers, Zsuzsanna György, Erling Fjelldal, Anna Szabó, Paul Eric Aspholm, Andrzej Pedryc
Genetic Diversity Of Golden Root (Rhodiola Rosea L.) In Northern Norway Based On Recently Developed Ssr Markers, Zsuzsanna György, Erling Fjelldal, Anna Szabó, Paul Eric Aspholm, Andrzej Pedryc
Turkish Journal of Biology
Roseroot (Rhodiola rosea L.), an adaptogenic herb, has received increased attention in recent years. The genetic diversity of roseroot was studied with simple sequence repeat (SSR) markers, which have not been widely used so far. Plants were collected in Finnmark County, Norway, from 10 habitats. Eight recently developed microsatellite (SSR) markers were used to assess genetic diversity. However, only 4 SSR markers were informative during this study. The primer pairs for these 4 SSR markers produced 20 fragments with an average of 5 putative alleles per locus. Observed heterozygosity was 1.0 at each locus, whereas expected heterozygosity ranged from 0.60 …
Purification And Characterization Of Endoxylanase Xln-2 From Aspergillus Niger B03, Georgi Dobrev, Boriana Zhekova
Purification And Characterization Of Endoxylanase Xln-2 From Aspergillus Niger B03, Georgi Dobrev, Boriana Zhekova
Turkish Journal of Biology
An extracellular multiple form of endoxylanase was isolated from the xylanolytic complex of Aspergillus niger B03. The enzyme was purified to a homogenous form using ultrafiltration, anion exchange chromatography, and gel filtration. It was a nonglycosylated protein with a molecular weight of 20,000 Da as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and 21,000 Da as determined by gel filtration. The optimal pH for the enzyme action was 5.0 and the optimal temperature was 55 °C. Endoxylanase stability was significantly improved in the presence of glycerol and sorbitol. The enzyme activity was activated by Mn^{2+} and Co^{2+}, and it was …
Comparison Of Three Yarrowia Lipolytica Strains For Lipase Production: Nbrc 1658, Ifo 1195, And A Local Strain, Özgür Kebabci, Ni̇lüfer Ci̇hangi̇r
Comparison Of Three Yarrowia Lipolytica Strains For Lipase Production: Nbrc 1658, Ifo 1195, And A Local Strain, Özgür Kebabci, Ni̇lüfer Ci̇hangi̇r
Turkish Journal of Biology
Lipase production was examined with 3 strains of Yarrowia lipolytica: NBRC 1658, IFO 1195, and a local strain. All strains were obtained from Hacettepe University's Department of Food Engineering. The addition of nitrogen and carbon sources and the effects of initial pH and temperature parameters were studied. For all 3 strains, the growth temperature range was 20-40 °C, and maximum growth was determined at 30 °C. The initial pH range was 6-9 for growth and 3-6 for lipase production. Maximum growth results were detected as follows: Y. lipolytica NBRC 1658 at 30 °C and pH 8, Y. lipolytica IFO 1195 …
Prevalence Of The Ivs1(+1)G->A And 35delg Mutations In The Gjb2 Gene Of Turkish Patients With Nonsyndromic Hearing Loss, Tüzün Arik Biyikli
Prevalence Of The Ivs1(+1)G->A And 35delg Mutations In The Gjb2 Gene Of Turkish Patients With Nonsyndromic Hearing Loss, Tüzün Arik Biyikli
Turkish Journal of Biology
GJB2 encodes connexin 26, a gap junction protein that is assumed to be a component of the potassium recycling pathway in the inner ear. Loss or malfunction of these gap junctions, as might be reflected by mutations in GJB2, may disrupt potassium movement from the hair cells through the supporting cell network to the endolymph, leading to hearing impairment. One mutation, the deletion of 1 guanosine residue from a stretch of 6 between nucleotide positions 30 and 35 (35delG) at codon 10, is the most common deafness-causing allelic variant of GJB2 in sporadic patients and autosomal recessive families. Mutations in …
Anti-Inflammatory Effects Of Different Extracts From Three Salvia Species, Eli̇f Çadirci, Hali̇s Süleyman, Peri̇han Gürbüz, Ayşe Uz, Zühal Güvenalp, Lütfi̇ye Ömür Demi̇rezer
Anti-Inflammatory Effects Of Different Extracts From Three Salvia Species, Eli̇f Çadirci, Hali̇s Süleyman, Peri̇han Gürbüz, Ayşe Uz, Zühal Güvenalp, Lütfi̇ye Ömür Demi̇rezer
Turkish Journal of Biology
Salvia L. species have been used for the treatment of various inflammatory ailments in traditional medicine. In order to evaluate this ethnobotanical information, water, methanol, n-butanol, acetone, and chloroform extracts from 3 Salvia species (S. fruticosa, S. verticillata, and S. trichoclada) were screened for their anti-inflammatory activity using in vivo experimental models in rats. For this purpose a carrageenan-induced inflammatory paw edema model was used. All extracts demonstrated anti-inflammatory activities; however, n-butanol extract of Salvia fruticosa (syn. S. triloba), which is known as Turkish sage, was found to be the most active. It can be expected that the active flavonoids, …
Protective Effects Of \Beta-Carotene And Silymarin On Human Lymphocytes, Erkan Yurtcu, Ezgi̇ Kasapoğlu, Feri̇de İffet Şahi̇n
Protective Effects Of \Beta-Carotene And Silymarin On Human Lymphocytes, Erkan Yurtcu, Ezgi̇ Kasapoğlu, Feri̇de İffet Şahi̇n
Turkish Journal of Biology
Beta-carotene and silymarin have antioxidant properties against oxidative damage and are used as dietary supplements. The aim of this study was to assess the protective effects of \beta-carotene and silymarin on healthy human lymphocytes exposed to L-arginine-induced oxidative damage. Study samples were lymphocyte cultures set up from venous blood obtained from 6 healthy individuals (3 males and 3 females). Oxidative DNA damage was induced by L-arginine. \beta-Carotene and silymarin were added to the cultures as antioxidants to observe their protective effects, if any. Alkaline single-cell gel electrophoresis (SCGE) was used to evaluate DNA damage. While L-arginine generated genotoxic damage in …
Folk Medicines In The Villages Of Ilıca District (Erzurum, Turkey), Ufuk Özgen, Yusuf Kaya, Peter Houghton
Folk Medicines In The Villages Of Ilıca District (Erzurum, Turkey), Ufuk Özgen, Yusuf Kaya, Peter Houghton
Turkish Journal of Biology
The villages of Ilıca District in Erzurum Province, Turkey, were surveyed in terms of plants and animals used traditionally for medicinal purposes. Plants were compiled according to their families, botanical names, local names, usages, and applications. A total of 70 plant species belonging to 29 families are used by village people to treat a wide range of complaints, such as hemorrhoids, urinary system infections, stomach disorders, respiratory diseases, skin diseases, rheumatism, and jaundice. In addition, 3 animal species are used for the treatment of some diseases. The collected data were compared with those formerly recorded.
Cytotoxic And Antibacterial Activities Of Leaf Extracts Of Astragalus Gombiformis Pomel (Fabaceae) Growing Wild In Tunisia, Hassen Teyeb, Nahla Zanina, Mohamed Neffati, Wahiba Douki, Mohamed Fadhel Najjar
Cytotoxic And Antibacterial Activities Of Leaf Extracts Of Astragalus Gombiformis Pomel (Fabaceae) Growing Wild In Tunisia, Hassen Teyeb, Nahla Zanina, Mohamed Neffati, Wahiba Douki, Mohamed Fadhel Najjar
Turkish Journal of Biology
Many species of the genus Astragalus have been long used in folk medicine because of their biological properties. The aim of this study was to investigate the antibacterial and cytotoxic activities of extracts from the leaves of wild Astragalus gombiformis Pomel. Antibacterial activity was assayed against several common human pathogenic bacterial strains using the paper disk-agar diffusion method. Cytotoxicity was measured against human lung carcinoma cells using the colorimetric MTT assay. The strongest cytotoxic activity against the human A549 lung epithelial carcinoma cell line was detected for dichloromethane extracts at IC_{50} = 85 ± 21.7 µg/mL. The best incubation time …
The Growth And Reproduction Of White Bream (Blicca Bjoerkna L. 1758) In An Oligo-Mesotrophic Lake In Northwest Anatolia (Sapanca, Turkey), Hacer Canan Okgerman, Mahmut Elp, Si̇bel Atasağun
The Growth And Reproduction Of White Bream (Blicca Bjoerkna L. 1758) In An Oligo-Mesotrophic Lake In Northwest Anatolia (Sapanca, Turkey), Hacer Canan Okgerman, Mahmut Elp, Si̇bel Atasağun
Turkish Journal of Biology
Growth parameters and the reproduction period of white bream (Blicca bjoerkna L. 1758) sampled from Sapanca Lake were determined monthly between November 2000 and October 2001. Three hundred and fifty white bream specimens caught ranged in age from 1^+ to 9^+ years, and the majority of the samples consisted of individuals of the 5^+-year-old age group. The von Bertalanffy equation was determined as Lt = 22.169 (1 - exp {-0.215[t + 0.986]}), Ø': 2.02 for males and Lt = 31.917 (1 - exp {-0.122[t + 1.087]}), Ø': 2.10 for females. The length-weight relationship was estimated as W = 0.0046 TL …
Phosphate-Solubilizing Microbes And Their Occurrence In The Rhizospheres Of Piper Betel In Karnataka, India, Padmavathi Tallapragada, Usha Seshachala
Phosphate-Solubilizing Microbes And Their Occurrence In The Rhizospheres Of Piper Betel In Karnataka, India, Padmavathi Tallapragada, Usha Seshachala
Turkish Journal of Biology
Low phosphate solubility is one of the most important factors limiting the plant growth in Indian soils. Many microorganisms can enhance phosphate solubility, but little is known about the magnitude of their phosphorus-solubilizing ability. The native populations of phosphate-solubilizing bacteria and fungi were studied in different rhizospheric soil samples obtained from betel vine plants (Piper betel L.) in order to compare the results. The present study focuses on the phosphate-solubilizing capacity of bacteria and fungi in rhizospheric soil samples obtained from betel vine plants, revealing the dominance of Aspergillus species (26.1 mm) as major phosphate solubilizers, along with Bacillus subtilis …
Determination Of Mutagenic Potencies Of Aqueous Extracts Of Thermopsis Turcica By Ames Test, Recep Li̇man, Yasi̇n Eren, Di̇lek Akyil, Muhsi̇n Konuk
Determination Of Mutagenic Potencies Of Aqueous Extracts Of Thermopsis Turcica By Ames Test, Recep Li̇man, Yasi̇n Eren, Di̇lek Akyil, Muhsi̇n Konuk
Turkish Journal of Biology
Thermopsis turcica is an endemic species of Turkey popularly known as Eber Sarısı or Piyam; it is a member of the Thermopsis genus, which contains plants considered to be medicinal throughout the world. In this study, the mutagenic effect of aqueous extracts obtained from the leaves, root, stem, and flowers of T. turcica were evaluated by the Ames test, using Salmonella typhimurium TA97, TA98, TA100, and TA102 strains with and without an S9 activation system. The results obtained were analyzed statistically using SPSS for Windows, and the Mann-Whitney test was performed for comparisons. The results showed that all of the …
The Effects Of Seasonal Variation On The Fatty Acid Composition Of Total Lipid, Phospholipid, And Triacylglycerol In The Dorsal Muscle Of Capoeta Trutta Found In The Tigris River (Turkey), Eli̇f İpek Satar, Ersi̇n Uysal, Erhan Ünlü, Mehmet Başhan, Ali̇ Satar
The Effects Of Seasonal Variation On The Fatty Acid Composition Of Total Lipid, Phospholipid, And Triacylglycerol In The Dorsal Muscle Of Capoeta Trutta Found In The Tigris River (Turkey), Eli̇f İpek Satar, Ersi̇n Uysal, Erhan Ünlü, Mehmet Başhan, Ali̇ Satar
Turkish Journal of Biology
The effects of seasonal variation on the fatty acid composition of total lipid, triacylglycerol, and phospholipid in the dorsal muscle of Capoeta trutta were assessed by gas chromatographic method. A total of 18 different fatty acids were determined in the composition of C. trutta. The variations in fatty acid composition of total lipid, triacylglycerol, and phospholipid were recorded throughout the seasons. The total polyunsaturated fatty acids (PUFA) and monounsaturated fatty acids (MUFA) were the most important groups of total lipid. Total saturated fatty acid (SFA) and total MUFA were seen to increase from spring to summer while total PUFA decreased …
Phylogenetic Characterization, Antimicrobial Susceptibilities, And Mechanisms Of Resistance In Bacteria Isolates From A Poultry Waste-Polluted River, Southwestern Nigeria, Olawale Olufemi Adelowo, Obasola Ezekiel Fagade
Phylogenetic Characterization, Antimicrobial Susceptibilities, And Mechanisms Of Resistance In Bacteria Isolates From A Poultry Waste-Polluted River, Southwestern Nigeria, Olawale Olufemi Adelowo, Obasola Ezekiel Fagade
Turkish Journal of Biology
Bacteria resistance to antibiotics has become a worldwide problem impacted partly by antibiotics use in livestock production. The present study investigated the pattern and mechanisms of resistance in 21 bacteria species isolated from a river used as a waste-dump by a poultry farm in Ogbomoso, southwestern Nigeria. Bacteria were isolated on nutrient agar supplemented with 50 µg/mL of tetracycline, their identities were determined by PCR amplification, and sequencing of their 16s rRNA genes and their susceptibility to 6 antimicrobials determined by broth dilution. The molecular basis of resistance among the isolates was also investigated by the PCR amplification of class …
Antimicrobial And Antioxidant Properties Of Artemisia L. Species From Western Anatolia, Şüra Baykan Erel, Gottfried Reznicek, Serdar Gökhan Şenol, Nefi̇se Ülkü Karabay Yavaşoğlu, Si̇bel Konyalioğlu, Ahmet Ulvi̇ Zeybek
Antimicrobial And Antioxidant Properties Of Artemisia L. Species From Western Anatolia, Şüra Baykan Erel, Gottfried Reznicek, Serdar Gökhan Şenol, Nefi̇se Ülkü Karabay Yavaşoğlu, Si̇bel Konyalioğlu, Ahmet Ulvi̇ Zeybek
Turkish Journal of Biology
Artemisia L. taxa (A. absinthium L., A. arborescens L., A. campestris L., A. scoparia Waldst. & Kit., A. santonicum L., and A. vulgaris L.) naturally distributed through western and southwestern Turkey were chosen as experimental materials in this study. Essential oils of the aerial parts of these Artemisia species were isolated by hydrodistillation and analyzed by gas chromatography-mass spectrometry. The major components were identified as sabinene in A. absinthium (17.56%), camphor in A. arborescens (33.39%), 1,2-dehydro acenaphthylene in A. campestris and A. scoparia (20.71% and 11.80%, respectively), and \alpha-thujone in A. vulgaris and A. santonicum (56.13% and 39.46%, respectively). Essential …
Changes In Seed Reserve Composition During Germination And Initial Seedling Development Of Safflower (Carthamus Tinctorius L.), Muhammet Tonguç, Ruzi̇ye Elkoyunu, Sabri̇ Erbaş, Yaşar Karakurt
Changes In Seed Reserve Composition During Germination And Initial Seedling Development Of Safflower (Carthamus Tinctorius L.), Muhammet Tonguç, Ruzi̇ye Elkoyunu, Sabri̇ Erbaş, Yaşar Karakurt
Turkish Journal of Biology
The present study was carried out to determine the changes in some reserve molecules of safflower seeds during germination and early seedling growth. The seeds of 2 safflower cultivars were used as plant material (Dinçer 5-118 and Montola 2000). Seeds were germinated for 72 h and observations were taken every 12 h throughout the study. Changes in total oil content (%), total protein content (%), total soluble sugar contents (TSS, mg g^{-1}), and reducing sugar contents (RS, mg g^{-1}) were determined for each cultivar during germination and seedling growth. The oil content of the cultivars decreased from 54.7% and 57.4% …
Antimicrobial Activity Of Poplar Propolis On Mutans Streptococci And Caries Development In Rats, Soley Arslan, Si̇bel Si̇li̇ci̇, Duygu Perçi̇n, Ayşe Nedret Koç, Özgür Er
Antimicrobial Activity Of Poplar Propolis On Mutans Streptococci And Caries Development In Rats, Soley Arslan, Si̇bel Si̇li̇ci̇, Duygu Perçi̇n, Ayşe Nedret Koç, Özgür Er
Turkish Journal of Biology
The influence of the different extracts of propolis and chlorhexidine on mutans streptococci and the development of dental caries in rats was examined in this study. Specifically, the antimicrobial effects of propolis extracts (including ethanol, methanol, chloroform, hexane, propylene glycol, and ethyl acetate) on Streptococcus mutans UA159 and Streptococcus sobrinus 6715 were studied. Minimal inhibitory concentration and minimal bactericidal concentration were determined using the National Committee for Clinical Laboratory Standards broth macrodilution method. In the animal study, the rats were infected with S. sobrinus and fed a cariogenic diet. They were treated topically twice a day with both ethanol extract …
Purification And Properties Of An Endoglucanase From Aspergillus Niger Vtcc-F021, Thi Hoa Pham, Dinh Thi Quyen, Ngoc Minh Nghiem
Purification And Properties Of An Endoglucanase From Aspergillus Niger Vtcc-F021, Thi Hoa Pham, Dinh Thi Quyen, Ngoc Minh Nghiem
Turkish Journal of Biology
An extracellular endoglucanase (EG) from Aspergillus niger VTCC-F021 was purified 2.09-fold to homogeneity with a yield of 18.4%. The enzyme had a molecular mass of 31 kDa and a specific activity of 14.122 U/mg protein. Optimum temperature was observed at 55 °C and optimum pH at 5. The enzyme was stable up to 50 °C and from pH 5 to 6 with residual activity >80% and 60%, respectively. The kinetic constants K_m and V_{max} determined for EG, with carboxyl methyl cellulose as a substrate, were 8.5815 mg CMC/mL and 20.121 U/mg protein, respectively. EDTA increased EG activity by 35% at …
A Comparison Of Major Taste- And Health-Related Compounds Of Vaccinium Berries, Jasminka Milivojevic, Vuk Maksimovic, Jelena Dragisic Maksimovic, Dragan Radivojevic, Milena Poledica, Sezai̇ Erci̇şli̇
A Comparison Of Major Taste- And Health-Related Compounds Of Vaccinium Berries, Jasminka Milivojevic, Vuk Maksimovic, Jelena Dragisic Maksimovic, Dragan Radivojevic, Milena Poledica, Sezai̇ Erci̇şli̇
Turkish Journal of Biology
The chemical fruit composition, phenolic profile, and corresponding total antioxidant activity of bilberry (Vaccinium myrtillus) and 2 commercial blueberry cultivars (Vaccinium corymbosum) were analyzed. Results from this study showed that cultivar Berkeley yielded the highest glucose and fructose contents (70.8 and 88.8 mg/g FW, respectively), and the sweetness index expressed a similar trend, achieving the highest value for cultivar Berkeley (279.2). Citric acid was the major organic acid in the berries tested. V. myrtillus yielded a total organic acids value 2-fold higher (0.35 mg/g FW) as well as the highest vitamin C content (25.8 mg/100 g FW). Among the flavonols …
Evaluation Of Nested Pcr Method For Diagnosis Of Meningitis Due To Neisseria Meningitidis And Haemophilus Influenzae, Zahra Hojjati Bonab, Safar Farajnia, Reza Ghotaslou, Elhameh Nikkhah
Evaluation Of Nested Pcr Method For Diagnosis Of Meningitis Due To Neisseria Meningitidis And Haemophilus Influenzae, Zahra Hojjati Bonab, Safar Farajnia, Reza Ghotaslou, Elhameh Nikkhah
Turkish Journal of Biology
Meningitis due to Neisseria meningitidis and Haemophilus influenzae is a common infectious disease among infants and children that can be fatal in the absence of rapid and proper diagnosis and treatment. Therefore, a rapid and sensitive method for diagnosis is a high priority. The aim of this study was to compare the performance of culture, conventional polymerase chain reaction (PCR), and nested PCR methods for diagnosis of meningitis caused by N. meningitidis and H. influenzae. A total of 106 cerebrospinal fluid samples were collected from infants and children suspected of having meningitis. A portion of these samples were used for …
Evaluation Of Antiinflammatory And Antinociceptive Effects Of Some Juniperus Species Growing In Turkey, Ni̇lüfer Orhan, Esra Akkol, Fatma Ergun
Evaluation Of Antiinflammatory And Antinociceptive Effects Of Some Juniperus Species Growing In Turkey, Ni̇lüfer Orhan, Esra Akkol, Fatma Ergun
Turkish Journal of Biology
Different parts and products of Juniperus species have been used as folk medicine for various infectious diseases, rheumatism, and wounds in Turkey. In order to evaluate this traditional usage, antiinflammatory and antinociceptive activities of leaf and berry extracts of 3 Juniperus species (J. excelsa, J. foetidissima, and J. sabina) were investigated. Additionally, antiinflammatory and antinociceptive activities of subextracts of J. oxycedrus subsp. oxycedrus (Joso) berries and leaves were evaluated. The p-benzoquinone-induced writhing test for antinociceptive activity and the carrageenan-induced hind paw edema model for antiinflammatory activity in mice were employed. Ethanol extract prepared from berries of J. foetidissima provided a …
Identification And Isolation Of Salt-Stress-Responsive Transcripts From Gossypium Arboreum L., Muhammad Naveed Shahid, Adil Jamal, Bushra Rashid, Beenish Aftab, Tayyab Husnain
Identification And Isolation Of Salt-Stress-Responsive Transcripts From Gossypium Arboreum L., Muhammad Naveed Shahid, Adil Jamal, Bushra Rashid, Beenish Aftab, Tayyab Husnain
Turkish Journal of Biology
Salinity has adverse effects on plant development and ultimately reduces production. Very few reports on the genes induced by salt stress in Gossypium arboreum are available. In this report salt-stress-responsive genes were screened from cotton leaves using the differential display (DD) technique, and gene expression was compared under control and salt stressed conditions. By using a 107 primer combination, 25 gene fragments were found to be up-regulated in response to salt stress. Out of 25 induced fragments, 12 were rejected as false positives after reamplification and quality control assay. The remaining 13 fragments were selected for cloning and genetic transformation. …
Antioxidant And Acetylcholinesterase Inhibitory Potential Of Arnica Montana Cultivated In Bulgaria, Dimitrina Zheleva Dimitrova, Vessela Balabanova
Antioxidant And Acetylcholinesterase Inhibitory Potential Of Arnica Montana Cultivated In Bulgaria, Dimitrina Zheleva Dimitrova, Vessela Balabanova
Turkish Journal of Biology
The antioxidant and acetylcholinesterase inhibitory potential of methanol extract from Arnica montana cultivated in Bulgaria was evaluated. For the determination of antioxidant activity 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) di-ammonium salt (ABTS) free radicals, and ferric reducing antioxidant power (FRAP) assay were used. Modified Ellman's colorimetric method was used for quantitative assessment of acetylcholinesterase inhibition potential. Butylated hydroxytoluene (BHT) and galantamine hydrobromide were used as positive controls, respectively. In addition, total polyphenols and tannins, flavonoids, hydroxycinnamic derivatives, and sesquiterpene lactones were determined using Folin-Ciocalteu reagent, AlCl_3, Na_2MoO_4, and m-dinitrobenzene, respectively. The results demonstrated that Arnica extract has strong antioxidant activity and moderate …