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Articles 991 - 1020 of 1681

Full-Text Articles in Biology

Colonization Of Mycobacterium Phlei In The Rhizosphere Of Wheat Grown Under Saline Conditions, Dilfuza Egamberdieva Jan 2012

Colonization Of Mycobacterium Phlei In The Rhizosphere Of Wheat Grown Under Saline Conditions, Dilfuza Egamberdieva

Turkish Journal of Biology

One of the natural reservoirs of potentially human-pathogenic bacteria is believed to be the rhizosphere. The aim of the present work was to test nontuberculous mycobacterium Mycobacterium phlei MbP18 for its ability to colonize the rhizosphere of wheat and to evaluate its effect on plant growth under saline conditions. In competitive wheat root tip colonization assays, M. phlei MbP18 showed poor competitive colonization of the wheat rhizosphere compared to the reference strain. The strain produced lipase, amylase, cellulase, and pectinase and grew well in the presence of high salt (up to 4% NaCl) and at high temperatures (up to 40 …


Biomarkers Of Petroleum Contamination In The Digestive Gland Of Perna Viridis (L.), Xivanand Narayan Verlecar, Karmabeer Jena, Somshekher Raghoba Desai, Priya Brata Das, Gagan Bihari Nityananda Chainy Jan 2012

Biomarkers Of Petroleum Contamination In The Digestive Gland Of Perna Viridis (L.), Xivanand Narayan Verlecar, Karmabeer Jena, Somshekher Raghoba Desai, Priya Brata Das, Gagan Bihari Nityananda Chainy

Turkish Journal of Biology

Oxidative stress responses were evaluated in the digestive gland tissue of Perna viridis exposed to water-accommodated fractions (WAFs) of petrol and diesel at 0.5% and 5% concentrations over 5-, 10-, and 15-day periods. Increased lipid peroxidation, protein carbonyl, DNA integrity, and hydrogen peroxide in WAF-exposed specimens are indicative of oxidative damage due to dissolved petroleum extracts. A consistent increase in superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, reduced glutathione, and ascorbic acid in WAF-exposed specimens signify the importance of antioxidants in protecting the cell against the oxidative damage arising from petroleum contamination. The responses of field specimens collected from …


Dats Suppresses Invasion Of Oral Squamous Cell Carcinoma Cell Lines By Reducing Matrix Metalloproteinase-9 Via Pi3k/Akt, Jian Bin Yang, Dong Yi Wei, Zhi Yan Wu, Su Hua Xu Jan 2012

Dats Suppresses Invasion Of Oral Squamous Cell Carcinoma Cell Lines By Reducing Matrix Metalloproteinase-9 Via Pi3k/Akt, Jian Bin Yang, Dong Yi Wei, Zhi Yan Wu, Su Hua Xu

Turkish Journal of Biology

Oral squamous cell carcinomas (OSCCs) are the most common type of oral cancers, and high morbidity and mortality are largely attributable to late-stage diagnosis. Despite significant advances in therapeutic strategies, the 5-year survival rate still remains low. Diallyl trisulfide (DATS), an oil-soluble organosulfur compound of garlic, was recently reported for its anticancer effect on several types of cancer cells in vitro; however, these anticancer effects are still unknown in human OSCC cells. In the present study, the effects of DATS on OSCC cell invasion in vitro were investigated. Human OSCC cell lines (HSC-2 and Ca9-22 cells) were treated with DATS …


Characterization Of Affinity Tag Features Of Recombinant Tetrahymena Thermophila Glutathione-S-Transferase Zeta For Tetrahymena Protein Expression Vectors, Cem Özi̇ç, Muhi̇tti̇n Arslanyolu Jan 2012

Characterization Of Affinity Tag Features Of Recombinant Tetrahymena Thermophila Glutathione-S-Transferase Zeta For Tetrahymena Protein Expression Vectors, Cem Özi̇ç, Muhi̇tti̇n Arslanyolu

Turkish Journal of Biology

Glutathione S-transferase (GST) is the one of most widely used affinity tags in biotechnology applications. The present study named a GST gene as the TtGSTz1 gene, characterized with the conserved glutathione binding motif (SSTSWRVRIAL) under a GST zeta subfamily from Tetrahymena thermophila. Phylogenetic analysis of the TtGstz1p protein sequence, with its orthologs from different GST classes, showed that it is a member of the unicellular GSTz monophyletic clade, which is positioned close to the bacterial GSTz clade. Seven codons of the TtGSTz1 gene were first engineered by introducing silent mutations (TAA > CAA or TAG > CAG) to the code for glutamine …


A Comparative Study On The Fatty Acid Composition Of The Oils From Five Bupleurum Species Collected From Turkey, Hati̇ce Taner Saraçoğlu, Gökhan Zengi̇n, Mehtap Akin, Apdurahman Aktümsek Jan 2012

A Comparative Study On The Fatty Acid Composition Of The Oils From Five Bupleurum Species Collected From Turkey, Hati̇ce Taner Saraçoğlu, Gökhan Zengi̇n, Mehtap Akin, Apdurahman Aktümsek

Turkish Journal of Biology

The oil content and fatty acid compositions of 5 Bupleurum species (B. intermedium, B. lancifolium, B. croceum, B. rotundifolium, and B. cappadocicum) were determined. Their oil contents ranged from 2.87% to 4.20%. Gas chromatography (GC) was used for the fatty acid composition. Twenty-one fatty acids were identified and quantified by GC. Oleic acid (C18:1) accounted for more than 50% of the total fatty acids in 3 of the Bupleurum species (B. intermedium, B. lancifolium, and B. rotundifolium). The total unsaturated fatty acid contents varied from 67.28% in B. cappadocicum to 87.06% in B. rotundifolium. The total saturated fatty acids represent …


Phytase Production From Lactobacillus Brevis, Meli̇s Sümengen, Sadik Di̇nçer, Ayşenur Kaya Jan 2012

Phytase Production From Lactobacillus Brevis, Meli̇s Sümengen, Sadik Di̇nçer, Ayşenur Kaya

Turkish Journal of Biology

In this study, the phytase from Lactobacillus brevis strains isolated from fermented food products was produced and characterized. Phytase production of this strain was determined by production of clear zones around the colonies on the sodium phytate containing medium, after 48 h of incubation at 30 °C. The molecular weight of the phytase from Lactobacillus brevis was estimated as 73 kDa and 34 kDa on SDS-PAGE. The optimum activity of Lactobacillus brevis extracellular and intracellular enzymes emerged at 120 °C, pH 3.4 and at 110 °C, pH 3.4, respectively. The extracellular enzyme from Lactobacillus brevis was 91% stable for 5 …


Genetic Variability In Grapevine Virus A From Vitis Vinifera L. × Vitis Labrusca L. In Sichuan, China, Jianhui Wang, Dehui Xi, Jianjun Liu, Keling Chen, Hongwen Li, Xiao Liu, Shu Yuan, Sezai̇ Erci̇şli̇, Honghui Lin Jan 2012

Genetic Variability In Grapevine Virus A From Vitis Vinifera L. × Vitis Labrusca L. In Sichuan, China, Jianhui Wang, Dehui Xi, Jianjun Liu, Keling Chen, Hongwen Li, Xiao Liu, Shu Yuan, Sezai̇ Erci̇şli̇, Honghui Lin

Turkish Journal of Biology

Grapevine virus A (GVA) has a quasispecies nature and is closely associated with the rugose-wood disease complex of Vitis vinifera L. In the different growing regions of table grape in Sichuan, southwest China, the incidence of GVA infection was found to be 16.9% by ELISA detection among 178 grape plants (Vitis vinifera L. × Vitis labrusca L.). The results of restriction fragment length polymorphism (RFLP) studies on the polymerase chain reaction (PCR) products of a total of 139 plasmids, cloned from 15 GVA isolates, suggested that the GVA isolates contained highly divergent variants. The plasmids from each GVA isolate that …


Molecular Characterization Of Phylogeneticrelationships In Fritillaria Species Inferred From Chloroplast Trnl-Trnf Sequences, Mi̇ne Türktaş, Meral Aslay, Erdal Kaya, Fahri̇ye Ertuğrul Jan 2012

Molecular Characterization Of Phylogeneticrelationships In Fritillaria Species Inferred From Chloroplast Trnl-Trnf Sequences, Mi̇ne Türktaş, Meral Aslay, Erdal Kaya, Fahri̇ye Ertuğrul

Turkish Journal of Biology

The genus Fritillaria embraces about 165 taxa in the family Liliaceae. In this study, the molecular phylogenetic relationships between 10 Fritillaria taxa were presented. Fritillaria spp. were collected from their natural habitats, and specimens with different morphological features were obtained via selective breeding. Specimens with the desired characters were presented as promising candidate cultivars for ornamental use. Phylogenetic analysis was based on DNA sequences of the chloroplast trnL-trnF region. The phylogeny was constructed using the neighbor joining, maximum parsimony, maximum likelihood, and Bayesian inference methods. The data showed that the examined Fritillaria spp. were evidently diverged into 2 Fritillaria subgenera. …


Bioremediation Of Endosulfan-Contaminated Soil By Using Bioaugmentation Treatment Of Fungal Inoculant Aspergillus Niger, Tejomyee Sadashiv Bhalerao Jan 2012

Bioremediation Of Endosulfan-Contaminated Soil By Using Bioaugmentation Treatment Of Fungal Inoculant Aspergillus Niger, Tejomyee Sadashiv Bhalerao

Turkish Journal of Biology

The persistence of endosulfan and intermediate metabolite endosulfan sulfate in the environment and their toxic effects on biota necessitate their removal. This study investigated the bioaugmentation of endosulfan-contaminated soil by fungal inoculant Aspergillus niger ARIFCC 1053. The influence of bioaugmentation by A. niger on endosulfan-contaminated soil was evaluated with the help of change in pH and released chloride, and by thin layer chromatography and gas chromatography analysis. Its effects on soil functionality were monitored by estimating dehydrogenase and arylsulfatase enzyme activities. The endosulfan degradation reached an undetectable level on day 15. The pH of the medium was nearly neutral (6.9) …


Genotoxicity Of Bovine Bone-Derived Microhydroxyapatite (Boneplus-Xs), Mehmet Topaktaş, Erman Sali̇h İsti̇fli̇, Mehmet Büyükleyla, Hasan Basri̇ İla, Tuba Canitezer, Mehmet Kürkçü Jan 2012

Genotoxicity Of Bovine Bone-Derived Microhydroxyapatite (Boneplus-Xs), Mehmet Topaktaş, Erman Sali̇h İsti̇fli̇, Mehmet Büyükleyla, Hasan Basri̇ İla, Tuba Canitezer, Mehmet Kürkçü

Turkish Journal of Biology

The aim of this study was to evaluate the genotoxic effect of a bovine bone-derived hydroxyapatite (HA), Boneplus-xs (B-P-XS). The genotoxicity of B-P-XS was investigated by examining the potential of the xenograft (B-P-XS) to induce gene mutation (evaluated using the Ames test), in vitro chromosome aberration (CA), and sister chromatid exchange (SCE) with tests in cultured human peripheral lymphocytes. Counts of reverse mutations in Salmonella typhimurium TA98 and TA100 strains were not found to be significantly increased after treatment with B-P-XS (P > 0.05). The presence of B-P-XS in peripheral blood culture for 24 and 48 h did not lead to …


Antihyperglycemic, Antioxidant And Cytotoxic Activities Of Alocasia Macrorrhizos (Linn.) Rhizomes Extract, Md Masudur Rahman, Md. Aslam Hossain, Saiful Alam Siddique, Kaishar Parvej Biplab, Md. Helal Uddin Jan 2012

Antihyperglycemic, Antioxidant And Cytotoxic Activities Of Alocasia Macrorrhizos (Linn.) Rhizomes Extract, Md Masudur Rahman, Md. Aslam Hossain, Saiful Alam Siddique, Kaishar Parvej Biplab, Md. Helal Uddin

Turkish Journal of Biology

Alocasia macrorrhizos (L.) (Araceae), a coarse and erect plant that is widely cultivated to be eaten as a vegetable throughout Bangladesh, has been reported to possess a number of medicinal properties. The antihyperglycemic, antioxidant, and cytotoxic effects of the methanol extract of A. macrorrhizos rhizomes were investigated. The extract was given at a single dose (250 and 500 mg/kg) in alloxan-induced hyperglycemic mice. The extract at 500 mg/kg produced a significant (P < 0.05) decrease in the blood glucose level (55.49%) at 8 h of treatment when compared with the control and was comparable with the reference drug, metformin. The extract was also subjected to antioxidant potentiality and brine shrimp lethality bioassays. The IC_{50} value of the extract was 693.0 \mug/mL and the LC_{50} was 188.14 \mug/mL.


Genetic Characterization Of Turkish Bread Wheat Landraces Based On Microsatellite Markers And Morphological Characters, Özlem Ateş Sönmezoğlu, Betül Bozmaz, Ahmet Yildirim, Nejdet Kandemi̇r, Nevzat Aydin Jan 2012

Genetic Characterization Of Turkish Bread Wheat Landraces Based On Microsatellite Markers And Morphological Characters, Özlem Ateş Sönmezoğlu, Betül Bozmaz, Ahmet Yildirim, Nejdet Kandemi̇r, Nevzat Aydin

Turkish Journal of Biology

Wheat landraces could be unique sources of favorable genes for agronomic traits like yield and insect, pest, and disease resistances, as well as quality characteristics that are important for breeding programs. Molecular marker systems offer great opportunities to characterize wheat accessions, which in particular are not morphologically identifiable. In this study, 20 bread wheat landraces collected from different regions of Turkey were characterized by using microsatellite markers (SSRs) and morphological characters. Seventeen morphological characters were used. Fifteen SSR primers were prescreened and the 7 most polymorphic primers were employed in characterization. The most polymorphic SSR loci were Xgwm 95 and …


Cardioprotective Effect Of Diazepam On Ischemia-Reperfused Isolated Hyperthyroid Rat Heart, Dareuosh Shackebaei, Atefeh Asadmobini, Mahvash Hesari, Maryam Vaezi, Siamak Shahidi Jan 2012

Cardioprotective Effect Of Diazepam On Ischemia-Reperfused Isolated Hyperthyroid Rat Heart, Dareuosh Shackebaei, Atefeh Asadmobini, Mahvash Hesari, Maryam Vaezi, Siamak Shahidi

Turkish Journal of Biology

Hyperthyroidism increases the vulnerability of the heart to ischemia-reperfusion (I/R) injury. Regarding the fact that diazepam also affects cardiac I/R injury, the current study was conducted to investigate the effect of diazepam on ischemia-reperfused isolated hyperthyroid rat heart. The animals were divided into 6 groups: the control, hyperthyroid, control diazepam (1 mg/kg), hyperthyroid diazepam (1 mg/kg), control diazepam (5 mg/kg), and hyperthyroid diazepam (5 mg/kg) groups. Langendorff-perfused rat hearts underwent 40 min of global ischemia and 45 min of reperfusion. Different cardiac parameters, including the left ventricular developed pressure, heart rate, coronary flow, and rate pressure product (RPP), were measured. …


Utilization Of Gold Nanostructures In Biomedical Applications, Gamze Tan, Mehmet Ali̇ Onur, Necdet Sağlam Jan 2012

Utilization Of Gold Nanostructures In Biomedical Applications, Gamze Tan, Mehmet Ali̇ Onur, Necdet Sağlam

Turkish Journal of Biology

Every living organism on earth owes its viability to its different sizes of nanostructures and the interaction of these structures at the nano size. Nanotechnology gives us an opportunity to understand nanoscale processes in living organisms and interfere with and manipulate them. Today, biocompatible nanosized structures are designed by applying developments in nanotechnology to biomedicine; thus, therapeutic agents are available to reach diseased tissues and even cells. However, it is essential that nanomaterials that would be used for therapeutic aims be targetable to diseased areas and have low toxicity. In addition, these nanomaterials must have high biocirculation and pharmacokinetic properties. …


Effect Of Exogenous Application Of Ascorbic Acid On Antioxidant Enzyme Activities, Proline Contents, And Growth Parameters Of Saccharum Spp. Hybrid Cv. Hsf-240 Under Salt Stress, Batool Ejaz, Zahoor Ahmad Sajid, Faheem Aftab Jan 2012

Effect Of Exogenous Application Of Ascorbic Acid On Antioxidant Enzyme Activities, Proline Contents, And Growth Parameters Of Saccharum Spp. Hybrid Cv. Hsf-240 Under Salt Stress, Batool Ejaz, Zahoor Ahmad Sajid, Faheem Aftab

Turkish Journal of Biology

The present study was undertaken to examine the effects of exogenous application of ascorbic acid (AA) through different modes on growth and associated biochemical parameters in Saccharum spp. hybrid cv. HSF-240, under salt stress. In a pot experiment, AA was applied through irrigation or foliar-spray at the concentrations of 0.1, 0.5, and 1 mM with or without 100 mM NaCl concentration. Vegetative growth measurements, antioxidant enzyme activities (POD and SOD), and protein and proline contents of plants were recorded to study the effects of these treatments. The presence of salt reduced the growth of sugarcane plants. The AA application not …


Biological Screening Of Various Medicinal Plant Extracts For Antibacterial And Antitumor Activities, Fatma Pehli̇van Karakaş, Arzu Yildirim, Arzu Türker Jan 2012

Biological Screening Of Various Medicinal Plant Extracts For Antibacterial And Antitumor Activities, Fatma Pehli̇van Karakaş, Arzu Yildirim, Arzu Türker

Turkish Journal of Biology

Bioassays of 2 types (antibacterial and antitumor) were performed to show the biological activities of 16 different plants grown in Bolu, Turkey: Clinopodium vulgare L. subsp. vulgare L., Salvia verticillata L. subsp. amasiaca (Frey & Bornm.) Bornm., Salvia tomentosa Mill., Mentha pulegium L., Melilotus officinalis (L.) Desr., Melilotus alba Desr., Medicago lupulina L., Galega officinalis L., Xeranthemum annuum L., Cichorium intybus L., Plantago lanceolata L., Plantago major L. subsp. major, Fumaria officinalis L., Galium palustre L., Echium vulgare L., and Sambucus nigra L. For each plant, 3 different extracts (aqueous, ethanol, and methanol) were obtained, and a total of 48 …


Effect Of Cell-Conditioned Media On Biomass Production Of Leishmania Parasites, Melahat Bagirova, Rabi̇a Çakir Koç, Adi̇l Allahverdi̇yev, Meli̇ke Ersöz Jan 2012

Effect Of Cell-Conditioned Media On Biomass Production Of Leishmania Parasites, Melahat Bagirova, Rabi̇a Çakir Koç, Adi̇l Allahverdi̇yev, Meli̇ke Ersöz

Turkish Journal of Biology

A large amount of parasite biomass production necessitates a higher volume of culture media, and this further increases the overall cost. For this reason, novel and alternative culture supplementary materials have been continuously investigated. The aim of this study was therefore to evaluate the effect of cell-conditioned media, collected as waste material during routine cell culture, on the growth and development of Leishmania parasites in vitro. In this study, for the first time, we show that cell-conditioned medium obtained from MCF-7 culture increased the growth of Leishmania parasites in vitro. These results show that the metabolites that are present in …


Isolation, Culturing And Characterization Of Rat Adipose Tissue-Derived Mesenchymal Stem Cells: A Simple Technique, Mehmet Ni̇yaz, Özer Ayli̇n Gürpinar, Serdar Günaydin, Mehmet Ali̇ Onur Jan 2012

Isolation, Culturing And Characterization Of Rat Adipose Tissue-Derived Mesenchymal Stem Cells: A Simple Technique, Mehmet Ni̇yaz, Özer Ayli̇n Gürpinar, Serdar Günaydin, Mehmet Ali̇ Onur

Turkish Journal of Biology

In this study, our aim was to develop a new simple technique for isolation of mesenchymal stem cells from adipose tissue. For this purpose, mesenchymal stem cells were isolated from rat adipose tissue by using the primary explant culture technique. When the cells became confluent, they were passaged 4 times by using the standard trypsinization method with trypsin/EDTA solution. Cells at second passage were characterized by using immunofluorescence staining against CD13 and CD29 markers. The results showed that these cultured cells were positive for CD13 and CD29 markers. Flow cytometry analysis was also done against CD29, CD90, CD54, MHC Class …


Assessment Of Antioxidant, Antibacterial, Antimycobacterial, And Antifungal Activities Of Some Plants Used As Folk Remedies In Turkey Against Dermatophytes And Yeast-Like Fungi, Di̇dem Deli̇orman Orhan, Berri̇n Özçeli̇k, Sanem Hoşbaş, Meci̇t Vural Jan 2012

Assessment Of Antioxidant, Antibacterial, Antimycobacterial, And Antifungal Activities Of Some Plants Used As Folk Remedies In Turkey Against Dermatophytes And Yeast-Like Fungi, Di̇dem Deli̇orman Orhan, Berri̇n Özçeli̇k, Sanem Hoşbaş, Meci̇t Vural

Turkish Journal of Biology

Twenty-seven aqueous extracts obtained from 21 plants used in the treatment of respiratory tract infections as folk remedies in Turkey were investigated for their relative total phenolic contents and antioxidant, antibacterial, antimycobacterial, and antifungal activities. Antibacterial and antifungal activities against dermatophytes and yeast-like fungi were determined using the microdilution method. Antibacterial activity of all extracts was more pronounced against gram-positive bacteria than against gram-negative bacteria. These extracts also exhibited higher antifungal activity against Candida krusei than ketaconazole. Six extracts displayed antidermatophytic activity against Epidermophyton floccosum, while only 3 extracts were highly active against Trichophyton rubrum. The extracts showed strong activity …


The Expression Of Gst Isoenzymes In Acinar Adenocarcinoma, Intraepithelial Neoplasia, And Benign Prostate Tissue: Correlation Of Clinical Parameters With Gst Isoenzymes, Gülçi̇n Şi̇mşek, Serpi̇l Oğuztüzün, Servet Güreşçi̇, Murat Kiliç, Ömer Faruk Bozkurt, Ali̇ Ünsal Jan 2012

The Expression Of Gst Isoenzymes In Acinar Adenocarcinoma, Intraepithelial Neoplasia, And Benign Prostate Tissue: Correlation Of Clinical Parameters With Gst Isoenzymes, Gülçi̇n Şi̇mşek, Serpi̇l Oğuztüzün, Servet Güreşçi̇, Murat Kiliç, Ömer Faruk Bozkurt, Ali̇ Ünsal

Turkish Journal of Biology

This study investigated the immunohistochemical staining characteristics of glutathione-S-transferase (GST) alpha, pi, mu, and theta in prostatic acinar adenocarcinoma (PCA), prostatic intraepithelial neoplasia (PIN), and benign prostatic tissues from 19 patients. Relationships between GST isoenzyme expression in benign, PIN, and PCA tissue were examined by the Wilcoxon signed-rank test and clinicopathological data were examined by the Spearman correlation rank test. When the benign, PIN, and PCA tissues from these cases were compared according to their staining intensity, GST alpha, pi, mu, and theta expressions in tumor cells were significantly lower than in benign epithelial cells (P < 0.05). The GST alpha class displayed the lowest level of expression in PIN and PCA. Expression of GST pi was lower in PCA tissue than in PIN and benign epithelial tissue (P < 0.05). We hypothesize that carcinogenesis in the prostate results from impaired cellular handling of mutagenic agents owing to reduction or loss of expression of multiple GST isoenzymes and other detoxifying and antimutagenesis agents. This study confirms the down-regulation of GST isoenzymes in PCA of the prostate and shows that the loss of GST isoenzyme expression is a phenotype associated with malignant transformation. There was no statistical relationship between GST isoenzyme expression and the clinicopathological data (age, Gleason score, and total serum prostate-specific antigen levels) (P > 0.05).


Management Of Root-Knot Disease In Eggplant Through The Application Of Biocontrol Fungi And Dry Neem Leaves, Mujeebur Rahman Khan, Fayaz Ahmad Mohiddin, Mohd Nadeem Ejaz, Mohd Mahmud Khan Jan 2012

Management Of Root-Knot Disease In Eggplant Through The Application Of Biocontrol Fungi And Dry Neem Leaves, Mujeebur Rahman Khan, Fayaz Ahmad Mohiddin, Mohd Nadeem Ejaz, Mohd Mahmud Khan

Turkish Journal of Biology

The incorporation of dry neem leaves into the soil significantly enhanced the yield of uninoculated eggplant, but aldicarb treatments at 4 kg a.i./ha proved to be phytotoxic. Inoculation with root-knot nematode Meloidogyne incognita (1500 J_2/plant) caused a significant decrease in both the plant growth (12.5%) and the yield (11.9%) of eggplant cv. Pusa Purple Round. Treatments involving neem leaves, Pochonia chlamydosporia, Paecilomyces lilacinus, Trichoderma harzianum, or aldicarb reduced the suppressive effect of the nematode, leading to a significant increase in the dry matter production and yield of inoculated plants compared to the inoculated control. Neem leaves induced a 19% increase …


Properties Of Biological Activity Of Ten Wild Almond (Prunus Amygdalus L.) Species, Ali Jahanban Esfahlan, Rashid Jamei Jan 2012

Properties Of Biological Activity Of Ten Wild Almond (Prunus Amygdalus L.) Species, Ali Jahanban Esfahlan, Rashid Jamei

Turkish Journal of Biology

To compare the antioxidant and antiradical activity of phenolic extracts of different species of wild almond kernels (including the brown skin), 10 wild almond species were selected from Shahindezh and Qasemloo Valley in West Azarbaijan Province, Iran. The fruits of these almonds were collected, their kernels were separated from their hulls and shells and then ground, and methanolic extracts were prepared from the kernels. The total phenolic and flavonoid contents were determined using the Folin-Ciocalteu method and a colorimetric method, respectively. The extracts were also evaluated on their reducing power and their capacity to scavenge for DPPH radicals. Significant differences …


The Histopathologic Effects Of Morus Alba Leaf Extract On The Pancreas Of Diabetic Rats, Jamshid Mohammadi, Prakash R. Naik Jan 2012

The Histopathologic Effects Of Morus Alba Leaf Extract On The Pancreas Of Diabetic Rats, Jamshid Mohammadi, Prakash R. Naik

Turkish Journal of Biology

Many traditional treatments have been recommended in the alternative system of medicine for the treatment of diabetes mellitus. Mulberry (Morus alba) is a nontoxic natural therapeutic agent shown to possess hypoglycemic, hypotensive, and diuretic properties. The purpose of this study was to examine the histopathologic effects of Morus alba leaf extract on the pancreas of diabetic rats. The animals were treated with mulberry leaf extract at dosages of 400 and 600 mg/kg body weight for 35 days. The various parameters studied included blood glucose, the relative body weight of the pancreas, the diameter of islets, and the number of \beta …


Thermostable \Alpha-Amylase From Moderately Halophilic Halomonas Sp. Aad21, Kami̇l Serkan Uzyol, Berna Sariyar Akbulut, Azi̇z Akin Deni̇zci̇, Di̇lek Kazan Jan 2012

Thermostable \Alpha-Amylase From Moderately Halophilic Halomonas Sp. Aad21, Kami̇l Serkan Uzyol, Berna Sariyar Akbulut, Azi̇z Akin Deni̇zci̇, Di̇lek Kazan

Turkish Journal of Biology

The moderately halophilic Halomonas sp. strain AAD21, which produces extracellular thermostable \alpha-amylase, was isolated from the Çamaltı Saltern area located in İzmir, Turkey. NaCl, carbon, and nitrogen sources in the growth medium were optimized to enhance \alpha-amylase yield. The highest enzyme yield was measured in the presence of 20% NaCl with peptone as the nitrogen and starch as the carbon sources in the fermentation broth. This microorganism was also found to utilize waste potato peel as a carbon source for \alpha-amylase production. Concentrations of carbon and nitrogen sources were optimized using a statistical approach, and \alpha-amylase activity increased from 4.07 …


Antioxidant Activity And Luteolin Content Of Marchantia Polymorpha L., Alper Gökbulut, Basri̇ Satilmiş, Kadi̇r Batçioğlu, Barbaros Çeti̇n, Engi̇n Şarer Jan 2012

Antioxidant Activity And Luteolin Content Of Marchantia Polymorpha L., Alper Gökbulut, Basri̇ Satilmiş, Kadi̇r Batçioğlu, Barbaros Çeti̇n, Engi̇n Şarer

Turkish Journal of Biology

Antioxidant activity of the methanol and ethyl acetate extracts of Marchantia polymorpha L., which grows naturally in Turkey, was tested by DPPH and ABTS methods. According to the applied methods, both extracts exhibited moderate activity. Some phenolic compounds that could be responsible for the activity were investigated by RP-HPLC, qualitatively and quantitatively, in the methanolic extract of liverwort. While caffeic acid, chlorogenic acid, rutin, myricetin, quercetin, and kaempferol were not found in the extract, luteolin as the liable antioxidant agent was determined as 0.0052 ± 0.0002% among the investigated compounds.


Cloning And Sequencing Of Coat Protein Gene Of Zucchini Yellow Mosaic Virus Isolated From Squash And Muskmelon In Turkey, Meryem Özer, Hi̇kmet Murat Si̇pahi̇oğlu, Mustafa Usta, Hakan Fi̇dan Jan 2012

Cloning And Sequencing Of Coat Protein Gene Of Zucchini Yellow Mosaic Virus Isolated From Squash And Muskmelon In Turkey, Meryem Özer, Hi̇kmet Murat Si̇pahi̇oğlu, Mustafa Usta, Hakan Fi̇dan

Turkish Journal of Biology

The coat protein (CP) genes of the genomic RNA of 2 severe Turkish isolates of Zucchini yellow mosaic virus (ZYMV) from squash and muskmelon [ZYMV-Adana (Ad) and ZYMV-Ahlat (Ah), respectively] were cloned, and their complete nucleotide sequences and deduced amino acids were determined. The analysis revealed that both Turkish ZYMV-CP genes contained 837 nucleotides encoded for a CP of about 31.2 kDa. Phylogenetic trees based on nucleic acid sequences were constructed by the neighbor joining and unweighted pair group mean arithmetic (UPGMA) methods with 100 bootstrap replicates. A high degree of homology was detected between the 2 Turkish isolates on …


A Study On The Characterization And Pathogenicity Of Bacteria From Lymantria Dispar L. (Lepidoptera: Lymantriidae), İsmai̇l Demi̇r, Emi̇ne Eryüzlü, Zi̇hni̇ Demi̇rbağ Jan 2012

A Study On The Characterization And Pathogenicity Of Bacteria From Lymantria Dispar L. (Lepidoptera: Lymantriidae), İsmai̇l Demi̇r, Emi̇ne Eryüzlü, Zi̇hni̇ Demi̇rbağ

Turkish Journal of Biology

Gypsy moth, Lymantria dispar L. (Lepidoptera: Lymantriidae), is one of the most serious pests affecting fruit, forest, industrial, and foliage plants worldwide. The aim of this study was to isolate and characterize pathogenic bacteria from this pest and determine their pathogenicity. L. dispar larvae were collected from various populations in the forest areas of the Black Sea region of Turkey in May and June of 2008 and 2009, and 9 culturable bacterial isolates were obtained from these larvae. Morphological and physiological characteristics of bacterial isolates were determined by conventional and routine techniques, and biochemical properties and metabolic enzyme profiles were …


Copper Bioremoval By Novel Bacterial Isolates And Their Identification By 16s Rrna Gene Sequence Analysis, Eli̇f Ölmezoğlu, Bi̇nnur Kiratli Herand, Mehmet Sali̇m Öncel, Kenan Tunç, Melek Özkan Jan 2012

Copper Bioremoval By Novel Bacterial Isolates And Their Identification By 16s Rrna Gene Sequence Analysis, Eli̇f Ölmezoğlu, Bi̇nnur Kiratli Herand, Mehmet Sali̇m Öncel, Kenan Tunç, Melek Özkan

Turkish Journal of Biology

Copper-tolerant bacteria were isolated from soil samples taken from a region where metal industries are located. After selecting 2 isolates with relatively higher bioremoval efficiency, the effects of increasing copper concentration, pH, and temperature on the bioremoval efficiency of the growing isolates were determined. Strain N1c and strain N5a showed maximal bioremoval efficiency of 82% and 75%, respectively, in 20 mg/L copper-containing medium at pH 6.8 and 30 °C. Although the isolates did not grow well at pH 5, a low amount of copper was removed at pH 5. Slow growth of N5a at pH 5 allowed for 26% copper …


Evaluation Of Biological Activities Of Goldmoss Stonecrop (Sedum Acre L.), Milan Stankovic, Ivana Radojevic, Milena Curcic, Sava Vasic, Marina Topuzovic, Ljiljana Comic, Snezana Markovic Jan 2012

Evaluation Of Biological Activities Of Goldmoss Stonecrop (Sedum Acre L.), Milan Stankovic, Ivana Radojevic, Milena Curcic, Sava Vasic, Marina Topuzovic, Ljiljana Comic, Snezana Markovic

Turkish Journal of Biology

In vitro antioxidant, antimicrobial, and antiproliferative activities of methanol, acetone, and ethyl acetate extracts of Sedum acre L. were determined. Their total phenolic contents as well as the concentrations of flavonoids were also evaluated. The total phenolic content was determined with Folin-Ciocalteu reagent and it ranged between 18.25 to 181.75 mg gallic acid/g. The concentration of flavonoids in various extracts of S. acre was determined by using a spectrophotometric method with aluminum chloride and the obtained results varied from 8.42 to 173.42 mg rutin/g. Antioxidant activity was monitored spectrophotometrically and expressed in terms of IC_{50} (\mug/mL), and its values ranged …


Genoprotective Potential Of Brassica Juncea (L.) Czern. Against Mercury-Induced Genotoxicity In Allium Cepa L., Sonia Sharma, Avinash Nagpal, Adarsh Pal Vig Jan 2012

Genoprotective Potential Of Brassica Juncea (L.) Czern. Against Mercury-Induced Genotoxicity In Allium Cepa L., Sonia Sharma, Avinash Nagpal, Adarsh Pal Vig

Turkish Journal of Biology

Brassica juncea (L.) Czern. is an important species of the family Brassicaceae with immense medicinal value. The main goal of this study was to investigate the antigenotoxic effects of different concentrations (0.1%-1.0%) of chloroform extract of B. juncea seeds on mercury-induced (0.75 ppm) genotoxic effects in root cells of Allium cepa. Three different modes of treatment were performed: pretreatment, posttreatment, and simultaneous treatment. For pretreatment, freshly emerged root tips were first treated with different concentrations of seed extract for 3 h and then treated with Hg for 3 h. For posttreatment, the roots were first treated with Hg and then …