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Articles 631 - 660 of 1681
Full-Text Articles in Biology
Analysis Of Global Micrornaome Profiles Of Caenorhabditis Elegans Oocytes And Early Embryos, Selen Güçlü Durgun, Ahmet Raşi̇t Öztürk, Arzu Atalay
Analysis Of Global Micrornaome Profiles Of Caenorhabditis Elegans Oocytes And Early Embryos, Selen Güçlü Durgun, Ahmet Raşi̇t Öztürk, Arzu Atalay
Turkish Journal of Biology
One of the most complex cell transformations in biology is the remodeling of a fertilized oocyte into a totipotent zygote. There is no transcription during this event, and therefore mRNAs and proteins accumulated in the oocyte during oogenesis are utilized until zygotic genome activation. In order to differentiate into somatic cells, the embryo must erase the totipotency signature coming from the oocyte cytoplasm in the somatic blastomeres. In this study, the microRNAome profiles of oocytes and early embryos before and after zygotic genome activation were identified. By using next-generation high-throughput pyrosequencing and miRNA microarray techniques, miRNAome profiles of Caenorhabditis elegans …
Human Adipose-Derived Stem Cells Differentiation Into Epidermal Cells And Interaction With Human Keratinocytes In Coculture, Alexandra Gaspar, Daniel Constantin, Ana-Maria Seciu, Lucia Moldovan, Oana Craciunescu, Elena Ganea
Human Adipose-Derived Stem Cells Differentiation Into Epidermal Cells And Interaction With Human Keratinocytes In Coculture, Alexandra Gaspar, Daniel Constantin, Ana-Maria Seciu, Lucia Moldovan, Oana Craciunescu, Elena Ganea
Turkish Journal of Biology
Current procedures used for skin injury reepithelization have yet to improve. At present, stem cell-based therapies provide great potential in repairing different damaged tissues due to the multipotent character of these cells. This study aimed to investigate the in vitro differentiation capacity of human adipose-derived stem cells (ASCs) into epidermal cells and to evaluate their interaction with HaCaT keratinocytes in coculture experimental models that mimicked the living environment of human skin. The mesenchymal phenotype of isolated stem cells was indicated by their spindle-shaped morphology and the expression of specific surface markers CD73, CD90, and CD105. After 21 days of cultivation …
Baculoviral Vector Loaded Mesenchymal Stem Cells As Efficient Gene Therapy Tools For Cancer Treatment, Sedef Hande Aktaş, Hakan Akbulut, Ayşe Eser Elçi̇n, Mahmut Parmaksiz, Arzu Keski̇n Aktan, Arzu Çöleri̇ Ci̇han, Yaşar Murat Elçi̇n, Fi̇kri̇ İçli̇
Baculoviral Vector Loaded Mesenchymal Stem Cells As Efficient Gene Therapy Tools For Cancer Treatment, Sedef Hande Aktaş, Hakan Akbulut, Ayşe Eser Elçi̇n, Mahmut Parmaksiz, Arzu Keski̇n Aktan, Arzu Çöleri̇ Ci̇han, Yaşar Murat Elçi̇n, Fi̇kri̇ İçli̇
Turkish Journal of Biology
Insufficient targeting of the therapeutic genes to tumor cells is one of the major reasons for failure in cancer gene therapy. Mesenchymal stem cells (MSCs) seem to be a good candidate as a carrier for gene therapy because of its selective tumor tissue-homing properties. In the current study, we constructed baculoviral vectors (BVs) carrying cytosine deaminase (CD) (BV-CD) or green fluorescence protein (GFP) genes (BV-GFP) and tested the transduction efficiency of the vectors in tumor and mesenchymal stem cells. We also tested the in vivo efficacy of the BV-CD vector in a colon cancer model. Our results showed that the …
Genetic Identification With Heteroplasmic Variations In Maternally Related Individuals In Forensic Cases, Ayşen Tezel, Ömer Çeti̇nkaya, Burçak Güzeltepe, Nurgül Kiliç
Genetic Identification With Heteroplasmic Variations In Maternally Related Individuals In Forensic Cases, Ayşen Tezel, Ömer Çeti̇nkaya, Burçak Güzeltepe, Nurgül Kiliç
Turkish Journal of Biology
Mitochondrial DNA (mtDNA), inherited from the mother, is used in a large variety of areas. In order to evaluate the connection between an incident and the victim or suspect in the analysis of criminal cases, DNA was obtained from buccal swabs sampled from 21 volunteers related through the mother's generation. Using Seqscape (v2.5.5) in an ABI 3100x1 capillary electrophoresis system, mtDNA sequence analyses were performed and compared to the revised Cambridge Reference Sequence. Individual mtDNA variations were detected. In three individuals, point heteroplasmy was observed. The results were evaluated according to the mtDNA typing guidelines established by the International Society …
In Silico Genome-Wide Identification And Analysis Of Microsatellite Repeats In The Largest Rna Virus Family (Closteroviridae), Biju George, Binu George, Mayur Awasthi, Ram Nageena Singh
In Silico Genome-Wide Identification And Analysis Of Microsatellite Repeats In The Largest Rna Virus Family (Closteroviridae), Biju George, Binu George, Mayur Awasthi, Ram Nageena Singh
Turkish Journal of Biology
Microsatellites are known to exhibit ubiquitous presence across all kingdoms of life, including viruses. Members of the family Closteroviridae are the largest RNA viruses and severely affect different agricultural crops worldwide, including1397645907citrus, grapevine, and vegetables. Here we identified and systematically analyzed the nature and distribution of both simple and complex microsatellites present in the complete genome of 36 species belonging to Closteroviridae. Our results showed, in all analyzed genomes, that neither genome size nor GC content had any influence on number, relative abundance, or relative density of microsatellites. For each genome, dinucleotide repeats were found to be highly predominant and …
Comparative And Phylogenetic Analysis Of Zinc Transporter Genes/Proteins In Plants, Recep Vatansever, İbrahi̇m İlker Özyi̇ği̇t, Ertuğrul Fi̇li̇z
Comparative And Phylogenetic Analysis Of Zinc Transporter Genes/Proteins In Plants, Recep Vatansever, İbrahi̇m İlker Özyi̇ği̇t, Ertuğrul Fi̇li̇z
Turkish Journal of Biology
Zinc is an important catalytic element for more than 300 enzymes and plays a structural role in the stabilization of many proteins. Protein domain analysis showed that identified Zn transporter proteins belong to the ZIP protein family (PF02535). Zn transporter sequences were found to have similar molecular weights (33.1-51.4 kDa) and amino acid lengths (306-478 amino acids) with 5.31-8.92 pI values. Subcellular localization of Zn transporters was predicted as the plasma membrane. They had 6-9 putative transmembrane domains with a variable region between TM-3 and TM-4, which could contain a potential histidine-rich metal-binding domain. Moreover, alignment analysis showed that the …
A Small Intron Of Drosophila Gamma Carboxylase, Not Predictable In Silico For Mammalian Splicing Machinery, Is Spliced Improperly In Human Cells, Solmaz Moniri Javadhesari, Alireza Zomorodipour, Spideh Valimehr
A Small Intron Of Drosophila Gamma Carboxylase, Not Predictable In Silico For Mammalian Splicing Machinery, Is Spliced Improperly In Human Cells, Solmaz Moniri Javadhesari, Alireza Zomorodipour, Spideh Valimehr
Turkish Journal of Biology
The splicing of two small introns of 58 and 67 nucleotides (nts) of the Drosophila gamma carboxylase (DγC) in cultured human cells was examined. The two introns are below the minimum size, which was proposed as a requirement for an intron to be spliced in human cells. None of the well-known software examined was able to provide a proper prediction for the DγC intron 1 in a mammalian host. Our in vivo experiments recognized only donor splice sites (not the acceptor site) of the DγC intron 1 by the mammalian cell, which, together with a new splicing acceptor site, generated …
Immunohistochemical Identification Of Aquaporin 2 In The Kidneys Of Wild Boars (Sus Scrofa), Katarzyna Michalek, Ewa Czerniawska-Piatkowska, Marta Grabowska, Maria Laszczynska
Immunohistochemical Identification Of Aquaporin 2 In The Kidneys Of Wild Boars (Sus Scrofa), Katarzyna Michalek, Ewa Czerniawska-Piatkowska, Marta Grabowska, Maria Laszczynska
Turkish Journal of Biology
No abstract provided.
Machilus Thunbergii Extract Inhibits Inflammatory Response Inlipopolysaccharide-Induced Raw264.7 Murine Macrophages Viasuppression Of Nf-$\Kappa$B And P38 Mapk Activation, Shu-Ju Wu, Wen-Bin Len, Chien-Yi Huang, Chian-Jiun Liou, Wen-Chung Huang, Chwan-Fwu Lin
Machilus Thunbergii Extract Inhibits Inflammatory Response Inlipopolysaccharide-Induced Raw264.7 Murine Macrophages Viasuppression Of Nf-$\Kappa$B And P38 Mapk Activation, Shu-Ju Wu, Wen-Bin Len, Chien-Yi Huang, Chian-Jiun Liou, Wen-Chung Huang, Chwan-Fwu Lin
Turkish Journal of Biology
Machilus thunbergii Sieb. and Zucc. (Lauraceae) is a medicinal plant used to treat edema and pain in China and Taiwan. The anti-inflammatory effects of M. thunbergii were not clear. In this study, we evaluated the anti-inflammatory effects of M. thunbergii extract in LPS-stimulated RAW 264.7 cells. The cells were pretreated with various doses (12.5 μg/mL-100 μg/mL) of M. thunbergii extract and activated with LPS. We found that 25 μg/mL M. thunbergii extract inhibited nitric oxide (NO), prostaglandin E2 (PGE2), inducible nitric oxide synthetase (iNOS), and cyclooxygenase-2 (COX-2) expression, as well as levels of proinflammatory cytokines including interleukin (IL)-6 and tumor …
Epigallocatechin 3-Gallate Applications On Ht-29 And Mcf-7 Cell Lines Andevaluation Of Tumor Suppressor Gene Methylation, Yunus Kasim Terzi̇, Özge Özer Kaya, Özlem Darcansoy İşeri̇, Zerri̇n Çeli̇k, Feri̇de İffet Şahi̇n
Epigallocatechin 3-Gallate Applications On Ht-29 And Mcf-7 Cell Lines Andevaluation Of Tumor Suppressor Gene Methylation, Yunus Kasim Terzi̇, Özge Özer Kaya, Özlem Darcansoy İşeri̇, Zerri̇n Çeli̇k, Feri̇de İffet Şahi̇n
Turkish Journal of Biology
No abstract provided.
Redox Gene Expression Of Adipose-Derived Stem Cells In Response To Soft Hydrogel, Fahsai Kantawong, Thasaneeya Kuboki, Satoru Kidoaki
Redox Gene Expression Of Adipose-Derived Stem Cells In Response To Soft Hydrogel, Fahsai Kantawong, Thasaneeya Kuboki, Satoru Kidoaki
Turkish Journal of Biology
No abstract provided.
Eukaryotic Expression, Purification, Identification, And Tissuedistribution Of Porcine Pid1, Xiaoling Chen, Huan Wang, Bo Zhou, Zhiqing Huang, Gang Jia, Guangmang Liu, Hua Zhao
Eukaryotic Expression, Purification, Identification, And Tissuedistribution Of Porcine Pid1, Xiaoling Chen, Huan Wang, Bo Zhou, Zhiqing Huang, Gang Jia, Guangmang Liu, Hua Zhao
Turkish Journal of Biology
No abstract provided.
The Effect Of Mart Gene On Biofilm Production Of Salmonella Typhimurium, Burcu Yener İlçe, Nefi̇se Akçeli̇k
The Effect Of Mart Gene On Biofilm Production Of Salmonella Typhimurium, Burcu Yener İlçe, Nefi̇se Akçeli̇k
Turkish Journal of Biology
No abstract provided.
Improvement Of Seed Germination And In Vitro Propagation Of Bupleurum Latissimum Nakai Through Embryogenesis, Kee Hwa Bae
Improvement Of Seed Germination And In Vitro Propagation Of Bupleurum Latissimum Nakai Through Embryogenesis, Kee Hwa Bae
Turkish Journal of Biology
No abstract provided.
Cdk Inhibitors-Induced Ssat Expression Requires Nf?B And Ppar? Inmcf-7 Breast Cancer Cells, Pinar Obakan, Şeyma Yildirim, Mert Burak Öztürk, Özge Berrak, Ajda Çoker Gürkan, Eli̇f Damla Arisan, Zeynep Narçi̇n Ünsal
Cdk Inhibitors-Induced Ssat Expression Requires Nf?B And Ppar? Inmcf-7 Breast Cancer Cells, Pinar Obakan, Şeyma Yildirim, Mert Burak Öztürk, Özge Berrak, Ajda Çoker Gürkan, Eli̇f Damla Arisan, Zeynep Narçi̇n Ünsal
Turkish Journal of Biology
The cyclin-dependent kinase (CDK) inhibitors purvalanol and roscovitine are therapeutic agents that control cell proliferation through regulating cell-cycle machinery. They also affect polyamine (PA) metabolism, which is activated in malignant tissues. Therefore, PA catabolism became a remarkable target in cancer therapies. Induction of the PA catabolic enzyme spermidine/spermine N1-acetyltransferase (SSAT) is under the control of transcription factors such as NF?B and PPARγ. The purpose of this study was to investigate the therapeutic potential of CDK inhibitors in combination with PAs in MCF-7 breast cancer cells. In order to understand the involvement of PA catabolic enzyme SSAT in this process we …
Identification And Evolutionary Genomics Of Novel Ltr Retrotransposons In Brassica, Faisal Nouroz, Shumaila Noreen, John Seymour Heslop-Harrison
Identification And Evolutionary Genomics Of Novel Ltr Retrotransposons In Brassica, Faisal Nouroz, Shumaila Noreen, John Seymour Heslop-Harrison
Turkish Journal of Biology
No abstract provided.
Discovery Of Dhn3 Variants In Wild Barley (Hordeum Spontaneum) Byhigh-Resolution Melting (Hrm) Technology, Cüneyt Uçarli, Nazaret Poyraz, Ayşe Fi̇li̇z Gürel
Discovery Of Dhn3 Variants In Wild Barley (Hordeum Spontaneum) Byhigh-Resolution Melting (Hrm) Technology, Cüneyt Uçarli, Nazaret Poyraz, Ayşe Fi̇li̇z Gürel
Turkish Journal of Biology
No abstract provided.
Effects Of A Western-Style Diet High In Cholesterol And Saturated Faton The Rabbit Exocrine Pancreas, María Alba Martínez Burgos, Maria Dolores Yago Torregrosa, Maria Dolores Mesa García, Maria Belen López Millán, Jesús Alcalá Bejarano Carrillo, Ana Rivas Velasco, Ángel Gil Hernández, Mariano Mañas Almendros, Emilio Martínez De Victoria Muñoz
Effects Of A Western-Style Diet High In Cholesterol And Saturated Faton The Rabbit Exocrine Pancreas, María Alba Martínez Burgos, Maria Dolores Yago Torregrosa, Maria Dolores Mesa García, Maria Belen López Millán, Jesús Alcalá Bejarano Carrillo, Ana Rivas Velasco, Ángel Gil Hernández, Mariano Mañas Almendros, Emilio Martínez De Victoria Muñoz
Turkish Journal of Biology
No abstract provided.
Effects Of Squirting Cucumber (Ecballium Elaterium) Fruit Juice Onagrobacterium Tumefaciens-Mediated Transformation Of Plants, Sancar Fati̇h Özcan, Mustafa Yildiz, Hussein Abdullah Ahmed Ahmed, Muhammad Aasim
Effects Of Squirting Cucumber (Ecballium Elaterium) Fruit Juice Onagrobacterium Tumefaciens-Mediated Transformation Of Plants, Sancar Fati̇h Özcan, Mustafa Yildiz, Hussein Abdullah Ahmed Ahmed, Muhammad Aasim
Turkish Journal of Biology
No abstract provided.
Development Of Therapeutic Proteins: Advances And Challenges, Muhammad Sajid Hamid Akash, Kanwal Rehman, Muhammad Tariq, Shuqing Chen
Development Of Therapeutic Proteins: Advances And Challenges, Muhammad Sajid Hamid Akash, Kanwal Rehman, Muhammad Tariq, Shuqing Chen
Turkish Journal of Biology
Therapeutic proteins have shown to be effective against a variety of diseases. Pharmaceutical companies are progressively focusing on research using proteins in search of novel effective therapeutics. The significant attention by researchers has resulted in considerable advances in the production and usage of therapeutic proteins in recent years. In the current study we focused on the understanding of therapeutic proteins and their impact on the human health care system. Advancements in the field of biotechnology have increased and facilitated the production of therapeutically significant proteins to combat various fatal diseases. Although protein-based therapeutics have taken center stage in drug discovery …
Changes In Gene Expression In Sirt3 Knockout Liver Cells, Randa Tao, Jamie Leclerc, Kübra Yildiz, Seong-Hoon Park, Barbara Jung, David Gius, Özkan Özden
Changes In Gene Expression In Sirt3 Knockout Liver Cells, Randa Tao, Jamie Leclerc, Kübra Yildiz, Seong-Hoon Park, Barbara Jung, David Gius, Özkan Özden
Turkish Journal of Biology
The sirtuin (SIRT) gene family is reported to regulate critical intracellular processes from aging to cellular metabolism and repair. SIRT3 knockout (SIRT3-/-) mice develop receptor positive mammary tumors starting at 13 months and SIRT3 expression is decreased in human breast cancer samples as well as several other diseases. It is established that carcinogenesis results from the accumulation of multiple aberrant genetic events including the activation of oncogenes and inactivation of tumor-suppressor genes. To determine the potential early genomic events that may play a role in the tumor-permissive phenotype observed in SIRT3-/- cells, we compared gene expression profile in SIRT3-/- and …
Immobilized Periphytic Cyanobacteria For Removal Of Nitrogenous Compounds And Phosphorus From Shrimp Farm Wastewater, Sanjoy Banerjee, Helena Khatoon, Mohamed Shariff, Fatimah Yusoff
Immobilized Periphytic Cyanobacteria For Removal Of Nitrogenous Compounds And Phosphorus From Shrimp Farm Wastewater, Sanjoy Banerjee, Helena Khatoon, Mohamed Shariff, Fatimah Yusoff
Turkish Journal of Biology
Cyanobacteria can be used to remove nitrogenous compounds from wastewater, but a major bottleneck in the process is the separation of cyanobacterial biomass from the treated water discharge, which may cause eutrophication. The current study assessed the suitability of three periphytic cyanobacteria (Geitlerinema sp., Gloeotrichia sp., and Lyngbya sp.) isolated from shrimp ponds. These cyanobacteria were immobilized by self-adhesion to polyvinyl chloride sheets, forming mats, and were screened for their efficacy to reduce nitrogenous compounds and phosphorus. Among the three isolates, Geitlerinema sp. showed the highest reduction rate (98%) and was further tested using shrimp pond wastewater. The results showed …
Antibacterial And Cytotoxic Properties Of Boron-Containing Dental Composite, Selami̇ Demi̇rci̇, Mustafa Sarp Kaya, Ayşegül Doğan, Şaban Kalay, Nergi̇s Özlem Altin, Ayşen Yarat, Serap Hati̇ce Akyüz, Fi̇kretti̇n Şahi̇n
Antibacterial And Cytotoxic Properties Of Boron-Containing Dental Composite, Selami̇ Demi̇rci̇, Mustafa Sarp Kaya, Ayşegül Doğan, Şaban Kalay, Nergi̇s Özlem Altin, Ayşen Yarat, Serap Hati̇ce Akyüz, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
Secondary dental caries are one of the major reasons for restoration replacements. Incorporating antimicrobial properties into dental materials would limit the initiation and progression of dental caries. In the current study, dental composites having 1%, 5%, and 10% (w/w) sodium pentaborate pentahydrate were prepared and analyzed for their mechanical properties, degree of monomer conversion (DC) rate, antibacterial effects against Streptococcus mutans, and biocompatibility with human dental pulp stem cells (hDPSCs). Incorporation of boron into the composites significantly decreased flexural strength and DC in a dose-dependent manner, but the value for 1% boron-containing composite still remained within acceptable levels. Compressive strength …
Whole-Genome Dna Methylation Analysis In Cotton (Gossypium Hirsutum L.) Under Different Salt Stresses, Xuke Lu, Xiaojie Zhao, Delong Wang, Zujun Yin, Junjuan Wang, Weili Fan, Shuai Wang, Tianbao Zhang, Wuwei Ye
Whole-Genome Dna Methylation Analysis In Cotton (Gossypium Hirsutum L.) Under Different Salt Stresses, Xuke Lu, Xiaojie Zhao, Delong Wang, Zujun Yin, Junjuan Wang, Weili Fan, Shuai Wang, Tianbao Zhang, Wuwei Ye
Turkish Journal of Biology
No abstract provided.
Ploidy Stability Of Oryza Sativa. L Cv Ir64 Transformed With The Moth Bean P5cs Gene With Significant Tolerance Against Drought And Salinity, Arumugam Mohana Priya, Subramanian Radhesh Krishnan, Manikandan Ramesh
Ploidy Stability Of Oryza Sativa. L Cv Ir64 Transformed With The Moth Bean P5cs Gene With Significant Tolerance Against Drought And Salinity, Arumugam Mohana Priya, Subramanian Radhesh Krishnan, Manikandan Ramesh
Turkish Journal of Biology
Rice is one of the world's most important crops. Thus, it is necessary to improve it by genetic transformation. IR64 is a high-yielding and disease-resistant variety, but it is sensitive to abiotic stresses, mainly salinity and drought. Therefore, an efficient transformation system is a prerequisite for transforming this indica rice with a stress-tolerant gene, the potent osmolyte proline-coding P5CS gene of Vigna aconitifolia. Successful transgene integration was confirmed by polymerase chain reaction (PCR) and Southern blot analysis. A Mendelian pattern of transgene inheritance was also observed in the progeny plants. The individual transformants expressed significant tolerance over wild-type control plants, …
Feasibility And Assessment Of The Phytoremediation Potential Of Duckweed For Triarylmethane Dye Degradation With The Emphasis On Some Physiological Responses And Effect Of Operational Parameters, Samaneh Torbati
Turkish Journal of Biology
Phytoremediation is a low-cost and effective technology that removes pollutants, such as dye-containing effluents, from the environment through the use of plants. In the present study, the potential of Lemna minor L. for decolorization and degradation of the triarylmethane dye malachite green was investigated. The results revealed that the decolorization ability of the plant species is as high as 88%. The effect of some operational parameters (initial dye concentration, temperature, pH, and amount (weight) of plant) on the efficiency of biological decolorization process was determined. The metabolic fate of the dye was proposed by identifying 8 intermediate compounds produced during …
Diazoxide Attenuates Ros Generation And Exerts Cytoprotection Under Conditions Of Ros Overproduction In Rat Uterus Cells, Olga Vadzyuk, Sergiy Kosterin
Diazoxide Attenuates Ros Generation And Exerts Cytoprotection Under Conditions Of Ros Overproduction In Rat Uterus Cells, Olga Vadzyuk, Sergiy Kosterin
Turkish Journal of Biology
Reactive oxygen species (ROS) overproduction may severely affect cell functions and even provoke cell death. Diazoxide, an opener of ATP-sensitive potassium channels, induces pharmacological preconditioning in different types of cells. However, the target of action of diazoxide is not clear enough because this substance can activate ATP-sensitive potassium channels localized in mitochondria (mitoKATP) as well as in plasma membranes (KATP channels). It has not yet been established if diazoxide activates mitoKATP in uterus cells. Our objective was to examine the influence of diazoxide on ROS production and on the viability of rat uterus cells under oxidative stress conditions. Using an …
Aspects Of Mycorrhizal Colonization In Adaptation Of Sweet Marjoram (Origanum Majorana L.) Grown On Industrially Polluted Soil, Marieta Hristozkova, Maria Geneva, Ira Stancheva, Madlen Boychinova, Efrosina Djonova
Aspects Of Mycorrhizal Colonization In Adaptation Of Sweet Marjoram (Origanum Majorana L.) Grown On Industrially Polluted Soil, Marieta Hristozkova, Maria Geneva, Ira Stancheva, Madlen Boychinova, Efrosina Djonova
Turkish Journal of Biology
The current study reveals the effects of mycorrhization on heavy metal uptake and accumulation, antioxidant potential, and essential oil composition of Origanum majorana L., grown on soil polluted with industrial Cd and Pb. Two strains of Claroideoglomus claroideum (EEZ 54 and EEZ 55) were isolated from soil that is naturally rich in metals. EEZ 35 (Funneliformis mosseae) was isolated from a place with industrial contamination. The percentage of mycorrhizal colonization with the EEZ 54 strain was higher than those of the other strains; there was no significant difference between EEZ 55 and EEZ 35. The highest value of the total …
Influence Of Carbon Sources On Growth And Gc-Ms Based Metabolite Profiling Of Arnica Montana L. Hairy Roots, Maria Petrova, Ely Zayova, Ivayla Dincheva, Ilian Badjakov, Mariana Vlahova
Influence Of Carbon Sources On Growth And Gc-Ms Based Metabolite Profiling Of Arnica Montana L. Hairy Roots, Maria Petrova, Ely Zayova, Ivayla Dincheva, Ilian Badjakov, Mariana Vlahova
Turkish Journal of Biology
Arnica montana L. (Asteraceae) is an economically important herb that contains numerous valuable biologically active compounds accumulated in various parts of the plant. The effects of carbon sources (sucrose, maltose, and glucose) at different concentrations (1%, 3%, 5%, 7%, and 9%) on growth were studied and GC-MS based metabolite profiling of A. montana hairy roots was conducted. The optimal growth and biomass accumulation of transformed roots were observed on an MS nutrient medium containing 3% or 5% sucrose. GC-MS analysis of hairy roots of A. montana showed the presence of 48 compounds in polar fractions and 22 compounds in apolar …
Evaluation Of The Phytoremediation Capacity Of Lemna Minor L. In Crude Oil Spiked Cultures, Taylan Kösesakal, Vesi̇le Selma Ünlü, Oktay Külen, Abdülrezzak Memon, Bayram Yüksel
Evaluation Of The Phytoremediation Capacity Of Lemna Minor L. In Crude Oil Spiked Cultures, Taylan Kösesakal, Vesi̇le Selma Ünlü, Oktay Külen, Abdülrezzak Memon, Bayram Yüksel
Turkish Journal of Biology
Phytoremediation of freshwater contaminated with crude oil is a technology that can restore damaged freshwater areas. Lemna minor is a small vascular plant that reproduces rapidly, is sensitive to a wide variety of pollutants, and is easy to culture. This study aimed to evaluate the phytoremediation capacity of L. minor in crude oil spiked cultures. Cultivation was carried out for 7 days in a greenhouse with a natural photoperiod and in nutrient solutions containing 0.5%, 1%, 2%, and 3% crude oil. Extracts were analyzed using GC/MS and synchronous UV fluorescence spectroscopy. After a week of cultivation, the fresh weight of …