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Full-Text Articles in Biology

Biocontrol Of Fusarium Wilt By Bacillus Pumilus, Pseudomonas Alcaligenes And Rhizobium Sp. On Lentil, Mohd Sayeed Akhtar, Uzma Shakeel, Zaki A. Siddiqui Jan 2010

Biocontrol Of Fusarium Wilt By Bacillus Pumilus, Pseudomonas Alcaligenes And Rhizobium Sp. On Lentil, Mohd Sayeed Akhtar, Uzma Shakeel, Zaki A. Siddiqui

Turkish Journal of Biology

The present study examined the effects of Bacillus pumilus, Pseudomonas alcaligenes, and Rhizobium sp. on wilt disease caused by Fusarium oxysporum f. sp. lentis and on the growth of lentil. Inoculation with F. oxysporum caused significant wilting, and reduced plant growth, the number of pods, and nodulation. Inoculation with B. pumilus together with P. alcaligenes caused a greater increase in plant growth, number of pods, nodulation, and root colonization by rhizobacteria, and also reduced Fusarium wilting to a greater degree than did individual inoculation. Use of Rhizobium sp. resulted in a greater increase in plant growth, number of pods, and …


The Effect Of 4-Chloroindole-3-Acetic Acid On Some Growth Parameters In Mung Bean Under Cadmium Stress, Mohammad Mazid, Barket Ali, Shamsul Hayat, Aqil Ahmad Jan 2010

The Effect Of 4-Chloroindole-3-Acetic Acid On Some Growth Parameters In Mung Bean Under Cadmium Stress, Mohammad Mazid, Barket Ali, Shamsul Hayat, Aqil Ahmad

Turkish Journal of Biology

Seedlings of mung bean (Vigna radiata L. Wilczek) cv. T-44 grown on a sand culture were exposed to cadmium (Cd) (100 µM) on day 10 of growth. These seedlings were subsequently sprayed with deionized water (control) or 4-chloroindole-3-acetic acid (4-Cl-IAA) (10^{-6} M) on day 15 and were sampled 30 days after sowing to assess growth and metabolite content. Growth (length, and fresh and dry mass of roots and shoots) and the level of metabolites (chlorophyll, nitrogen, phosphorus, total sugar, and soluble protein) in the leaves significantly decrease in response to Cd stress; however, the 4-Cl-IAA (10^{-6} M) treatment, both in …


In Vitro Genotoxic Effects Of Benzoyl Peroxide In Human Peripheral Lymphocytes, Arzu Yavuz, Mehmet Topaktaş, Erman Sali̇h İsti̇fli̇ Jan 2010

In Vitro Genotoxic Effects Of Benzoyl Peroxide In Human Peripheral Lymphocytes, Arzu Yavuz, Mehmet Topaktaş, Erman Sali̇h İsti̇fli̇

Turkish Journal of Biology

The aim of the present research was to investigate the genotoxic potency of benzoyl peroxide (BPO), a whitener of flour and cheese, using in vitro sister chromatid exchange (SCE), chromosome aberration (CA), and micronucleus (MN) tests in human peripheral lymphocytes. Cells were exposed to 25, 50, 75, and 100 µg/mL concentrations of BPO for 24 and 48 h. BPO induced SCEs only at the highest concentration (100 µg/mL) for 48 h treatment. Furthermore, BPO induced CAs at 75 and 100 µg/mL concentrations for 24 h treatment, and at all concentrations for 48 h treatment. BPO induced CAs in a dose-dependent …


Brown Alga Sargassum Muticum Inhibits Proinflammatory Cytokines, Inos, And Cox-2 Expression In Macrophage Raw 264.7 Cells, Weon-Jong Yoon, Wook Jae Lee, Nam Ho Lee, Chang-Gu Hyun Jan 2010

Brown Alga Sargassum Muticum Inhibits Proinflammatory Cytokines, Inos, And Cox-2 Expression In Macrophage Raw 264.7 Cells, Weon-Jong Yoon, Wook Jae Lee, Nam Ho Lee, Chang-Gu Hyun

Turkish Journal of Biology

The present study was designed to elucidate the pharmacological and biological effects of Sargassum muticum extracts on the production of inflammatory mediators in macrophages. S. muticum was extracted with 80% EtOH. The extract was then successively partitioned with n-hexane, CH_2Cl_2, EtOAc, BuOH, and water. The results indicate that the CH_2Cl_2 fraction of S. muticum extract was an effective inhibitor of LPS-induced NO and PGE_2 production in RAW 264.7 cells. These inhibitory effects of the CH2Cl2 fraction of S. muticum included dose-dependent decreases in the expression of iNOS and COX-2 proteins and iNOS and COX-2 mRNA. To test the inhibitory effects …


Stimulatory Effects Of Ghrelin On Spontaneous Contractions In The Rat Myometrium, Alpaslan Dayangaç, Seli̇m Kutlu, Vahi̇t Konar Jan 2010

Stimulatory Effects Of Ghrelin On Spontaneous Contractions In The Rat Myometrium, Alpaslan Dayangaç, Seli̇m Kutlu, Vahi̇t Konar

Turkish Journal of Biology

Ghrelin, a recently isolated hormone, has been reported to have modulatory effects on smooth muscle contractility. In this study, we investigated the effects of ghrelin on spontaneous contractions in the rat uterus in vitro. Myometrium strips were removed from Wistar rats following decapitation and placed in a jacketed tissue bath. After initiation of spontaneous contractions, control contractions were recorded for 10 min and various concentrations of ghrelin were added to the tissue bath cumulatively. The Wilcoxon signed ranks test was used for statistical analysis. Application of ghrelin augmented the spontaneous contractions in myometrial strips. The mean peak amplitudes of contractions …


Production Of (S)-2'-Fluorophenylethan-1-Ol And (S)-3'- Fluorophenylethan-1-Ol By Alternaria Alternata Using Ram Horn Peptone In Microbial Growth Medium, Esabi̇ Başaran Kurbanoğlu, Namudar İzzet Kurbanoğlu, Kani̇ Zi̇lbeyaz Jan 2010

Production Of (S)-2'-Fluorophenylethan-1-Ol And (S)-3'- Fluorophenylethan-1-Ol By Alternaria Alternata Using Ram Horn Peptone In Microbial Growth Medium, Esabi̇ Başaran Kurbanoğlu, Namudar İzzet Kurbanoğlu, Kani̇ Zi̇lbeyaz

Turkish Journal of Biology

Enantiomerically pure, fluorinated compounds have an important role in medicinal chemistry. Alternaria alternata isolates were used for the synthesis of (S)-2^'-fluorophenylethan-1-ol 1b and (S)-3^'-fluorophenylethan-1-ol 2b. Biocatalytic syntheses of optically active 1b and 2b were achieved by asymmetric reduction of 2^'-fluoroacetophenone 1a and 3^'-fluoroacetophenone 2a in the batch culture of A. alternata containing ram horn peptone (RHP). The reaction conditions (pH, temperature, and agitation) that improve the conversion of the substrates were studied. The optimal reaction conditions were found as pH 6.0, agitation 250, temperature 32 °C and substrate 3 mmol/100 mL. The gram scale productions of 1b and 2b by …


The Effect Of Ascorbic Acid And H_2o_2 Treatment On The Stability Of Anthocyanin Pigments In Berries, Elham Nikkhah, Masoud Khaiamy, Reza Heidary, Ali Sorayaie Azar Jan 2010

The Effect Of Ascorbic Acid And H_2o_2 Treatment On The Stability Of Anthocyanin Pigments In Berries, Elham Nikkhah, Masoud Khaiamy, Reza Heidary, Ali Sorayaie Azar

Turkish Journal of Biology

Anthocyanins are natural pigments widely distributed in nature. Anthocyanin pigment molecules are a subclass of flavonoids. They are responsible for the red, purple, and blue colors observed in many flowers, fruits, and vegetables. Fruits and berries are the main sources of anthocyanins in nature. Anthocyanins are thought to contribute to the nutritive value of fruits and berries due to their antioxidative, anti-carcinogenic, anti-inflammatory, and anti-angiogenic properties. Anthocyanins can also improve the nutritional value of processed foods by preventing the oxidation of lipids and proteins. As such, identification of agents that can affect the stability of anthocyanins and the protective effect …


The Effects Of 2,4-Dichlorophenoxy Acetic Acid And Isoproturon Herbicides On The Mitotic Activity Of Wheat (Triticum Aestivum L.) Root Tips, Sanjay Kumar, Shashi Kiran Arya, B. K. Roy, Atul Kumar Singh Jan 2010

The Effects Of 2,4-Dichlorophenoxy Acetic Acid And Isoproturon Herbicides On The Mitotic Activity Of Wheat (Triticum Aestivum L.) Root Tips, Sanjay Kumar, Shashi Kiran Arya, B. K. Roy, Atul Kumar Singh

Turkish Journal of Biology

The effects of the herbicides 2,4-dichlorophenoxy acetic acid and isoproturon on 3 wheat (Triticum aestivum L.) varieties (HUW 234, HUW 468, and HUW 533) were studied with regards to mitotic abnormalities and chromosomal behavior. Pre-soaked seeds were treated with both herbicides at concentrations of 50-1200 ppm. Both 2,4-D and isoproturon were highly mito-inhibitory and induced chromosomal abnormalities, such as precocious movement, stickiness, and chromosome bridges, with and without laggards and fragments at the anaphase and telophase. The frequency of chromosomal anomalies in almost all the targets used was high at the maximum dose of both herbicides, individually as well as …


Genetic Relationships Of The Genera Onobrychis, Hedysarum, And Sartoria Using Seed Storage Proteins, Emi̇ne Arslan, Kuddi̇si̇ Ertuğrul Jan 2010

Genetic Relationships Of The Genera Onobrychis, Hedysarum, And Sartoria Using Seed Storage Proteins, Emi̇ne Arslan, Kuddi̇si̇ Ertuğrul

Turkish Journal of Biology

Fifteen species belonging to 3 genera (Onobrychis, Hedysarum and Sartoria), collected from different geographical regions of Turkey were studied for the polypeptide patterns of their seed storage proteins. The variability of seed storage proteins was analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). As many as 72 bands were scored and a dendrogram was constructed using UPGMA (unweighted pair group method with arithmetic mean). The dendrogram of the electrophoretic protein profiles of seeds showed 2 main clusters. While the first cluster with further subclusters included Onobrychis species, the second cluster included Sartoria and Hedysarum species present in 2 separate subclusters. …


The Superiority Of Cv ‘Rosea’ Over Cv ‘Alba’ Of Periwinkle (Catharanthus Roseus L.) In Alkaloid Production And Other Physiological Attributes, Mohd Idrees, M. Naeem, M. Masroor A. Khan Jan 2010

The Superiority Of Cv ‘Rosea’ Over Cv ‘Alba’ Of Periwinkle (Catharanthus Roseus L.) In Alkaloid Production And Other Physiological Attributes, Mohd Idrees, M. Naeem, M. Masroor A. Khan

Turkish Journal of Biology

A pot experiment was conducted during the winter season of 2006, on 2 cvs. of periwinkle (Catharanthus roseus L.), namely "rosea" (Pink) and "alba" (White), to investigate their performance in terms of growth, yield, and alkaloid production, as well as other physiological and biochemical attributes. The plants were harvested at 60, 90, and 150 days after planting (DAP) and the yield was recorded at 210 DAP. The results showed that "rosea" was superior to "alba" in plant height, total and individual leaf area, and fresh and dry weights at 90 DAP. Net photosynthetic rate, stomatal conductance, carbonic anhydrase activity and …


Characterization Of Essential Oils Of Some Salvia Species And Their Antimycobacterial Activities, Tüli̇n Aşkun, K. Hüsnü Can Başer, Gülendam Tümen, Mi̇ne Kürkçüoğlu Jan 2010

Characterization Of Essential Oils Of Some Salvia Species And Their Antimycobacterial Activities, Tüli̇n Aşkun, K. Hüsnü Can Başer, Gülendam Tümen, Mi̇ne Kürkçüoğlu

Turkish Journal of Biology

The compositions of the essential oils of 5 Turkish Salvia species, namely Salvia aucheri Bentham var. aucheri (endemic for Turkey), Salvia aramiensis Rech. fil., Salvia fruticosa Mill., Salvia tomentosa Mill., and Salvia verticillata L. subsp. amasiaca (Freyn & Bornm.) Bornm., were studied. Water distilled essential oils from the aerial parts of Salvia species from Turkey were analysed by GC and GC/MS. Salvia aucheri var. aucheri, Salvia aramiensis, and Salvia fruticosa oils have the same main constituent: 1,8-cineole (39.2%, 55.6%, and 52.8% respectively). \alpha-Pinene (25.1%), camphor (14.9%), and borneol (13.2%) were identified as the major components of Salvia tomentosa. The main …


Nutritive Value Of Edible Wild And Cultured Mushrooms, Sevda Kirbağ, Mehmet Akyüz Jan 2010

Nutritive Value Of Edible Wild And Cultured Mushrooms, Sevda Kirbağ, Mehmet Akyüz

Turkish Journal of Biology

The nutritive value of Pleurotus ostreatus (Jacq. ex Fr.) Kumm., Pleurotus sajor-caju (Fr.) Singer, and Agaricus bisporus (Large) Sign. was determined. Dry matter, moisture, crude ash, protein, fat, cellulose, organic matter, and nitrogen-free extract were 89.7%-90.3%, 9.7%-10.3%, 6.0%-13.7%, 27.8%-41.6%, 0.5%-1.3%, 10.0%-16.2%, 76.0%-84.0%, and 26.7%-36.8% of dry weight, respectively. Mineral element contents were 14.1-45.6 g K kg^{-1}, 0.2-1.2 g Ca kg^{-1} , 0.4-1.9 g Mg kg^{-1}, and 0.2-0.9 g Na kg^{-1} as macronutrients, and 176.5-838.0 mg Fe kg^{-1}, 35.0-46.0 mg Zn kg^{-1}, 4.8-65.4 mg Mn kg^{-1}, 6.5-21.5 mg Cu kg^{-1}, 0.0-11.5 mg Cr kg^{-1}, and 0.0-1.65 mg Cd kg^{-1} as micronutrients …


Germination Characteristics And Molecular Characterizations Of Some Wheat Varieties In Sulaimanyah By Ssr Marker, Nawroz Abdul-Razzak Tahir Jan 2010

Germination Characteristics And Molecular Characterizations Of Some Wheat Varieties In Sulaimanyah By Ssr Marker, Nawroz Abdul-Razzak Tahir

Turkish Journal of Biology

The genetic diversity of 11 bread and durum wheat varieties was estimated using germination characteristic and molecular analysis. The phenotypic data showed highly significant differences between cultivars for germination speed, germination percentage, seedling length, fresh weight, and dry weight. Simple sequence repeats (SSRs) were used to examine the genomic diversity of wheat (Triticum aestivum L. and Triticum durum L.). Eleven wheat varieties of diverse origins were analyzed with 12 selected SSRs to provide uniform and maximum genome coverage. A total of 18 bread wheat and 20 durum wheat polymorphic allelic variants were detected, ranging from 1 to 4 per locus …


Effects Of Chromium Vi Stress On Green Alga Ulva Lactuca (L.), Di̇lek Ünal, Nuray O. Işik, Atakan Sukatar Jan 2010

Effects Of Chromium Vi Stress On Green Alga Ulva Lactuca (L.), Di̇lek Ünal, Nuray O. Işik, Atakan Sukatar

Turkish Journal of Biology

In order to contribute to the understanding of its response to metal stress, Ulva lactuca (L.) was treated with different concentration of chromium (VI) (0.2, 0.5, 1, and 5 mM K2CrO4), and alterations in Fv/Fm ratio (maximal photochemical efficiency of PSII) and cell viability were investigated. Significant alterations of the maximal photochemical efficiency of PSII (Fv/Fm) ratio were observed in response to the increase in chromium concentration. The Fv/Fm ratio decreased in Ulva lactuca following 2 h treatment with 1 and 5 mM Cr(VI) solution. The cell viability was decreased in cells exposed to higher Cr(VI) concentrations. Thallus cell morphology …


Determination Of The Relationship Between Some Centaurea Species Based On Sds-Page, Tuna Uysal, Emi̇ne Arslan, Osman Tugay, Kuddi̇si̇ Ertuğrul Jan 2010

Determination Of The Relationship Between Some Centaurea Species Based On Sds-Page, Tuna Uysal, Emi̇ne Arslan, Osman Tugay, Kuddi̇si̇ Ertuğrul

Turkish Journal of Biology

The protein patterns of 47 Centaurea species from Turkey were obtained using SDS-PAGE methods. Relationships between Centaurea species and related genera were evaluated via use of a dendrogram. SDS-PAGE provided protein profiles for an important and applicable selection within Centaurea, particularly for the section Cheirolepis and its relatives. We determined that the technique could be used to resolve taxonomic and evolutionary problems in the genus Centaurea, as most of the Centaurea species studied exhibited special protein profiles and patterns.


Studies On The Shelf Life Of Bread Using Acidulants And Their Salts, Omer Mukhtar Tarar, Salim Ur-Rahman, Ghulam Mueen Ud Din, Mian Anjum Murtaza Jan 2010

Studies On The Shelf Life Of Bread Using Acidulants And Their Salts, Omer Mukhtar Tarar, Salim Ur-Rahman, Ghulam Mueen Ud Din, Mian Anjum Murtaza

Turkish Journal of Biology

White bread loaves prepared from wheat flour and the addition of different levels of acidulants, i.e. acetic and lactic acid with calcium propionate, were evaluated for their microbial shelf life as well as sensory quality characteristics during 96 h of storage. Among the sensory characteristics for the different treatments, volume of bread, color of crust, symmetry of form, crust characteristics, grain, and crumb color differed significantly, and the scores were 6.5-7.4, 5.8-6.8, 2.9-3.4, 2.7-3.1, 10.6-11.8, and 6.8-7.4, respectively, whereas evenness of bake, aroma, taste, and texture were not significantly different. Storage for 96 h affected the aroma, taste, texture, and …


A Study On The Morphology Of Argulus Foliaceus Lin., 1758 (Crustacea; Branchiura) Procured From Çavuşcu Lake (Central Anatolia-Turkey) With Scanning Electron Microscopy, Ali̇ Alaş, Ahmet Öktener, Kemal Solak Jan 2010

A Study On The Morphology Of Argulus Foliaceus Lin., 1758 (Crustacea; Branchiura) Procured From Çavuşcu Lake (Central Anatolia-Turkey) With Scanning Electron Microscopy, Ali̇ Alaş, Ahmet Öktener, Kemal Solak

Turkish Journal of Biology

In this study, 24 carps (Cyprinus carpio Lin.,1758) caught from Çavuşçu Lake (Konya; 36°41´N, 34°26´E) were examined for metazoan parasites. Infestation rate of Argulus foliaceus Lin.,1758 was found to be 45.8%. In addition, Argulus foliaceus was photographed using a scanning electron microscope (SEM) and a light microscope.


Aphrodisiac Activity And Curative Effects Of Pedalium Murex (L) Against Ethanol Induced Infertility In Male Rats, Gunasekaran Balamurugan, P. Muralidharan, Satyanarayana Polapala Jan 2010

Aphrodisiac Activity And Curative Effects Of Pedalium Murex (L) Against Ethanol Induced Infertility In Male Rats, Gunasekaran Balamurugan, P. Muralidharan, Satyanarayana Polapala

Turkish Journal of Biology

It has been suggested that chronic ethanol exposure may result in testicular damage and infertility in males. Petroleum ether extract of Pedalium murex, family Pedaliaceae (PEPM), is evaluated in this study for its ability to increase aphrodisiac activity and to cure ethanol induced germ cell damage and infertility in male rat models. Doses of 200 and 400 mg/kg of PEPM showed a significant increase (P < 0.01, P < 0.001) in mating and mounting behaviour. The effect on fertility factors such as total body weight, percentage of pregnancy, litter size were also significantly increased (P < 0.01) in comparison with the ethanol-treated group. Significant increases in sperm motility and count were observed in PEPM-treated groups in a dose-dependent manner (P < 0.01; P < 0.001) as compared with the ethanol-treated group. Similarly, reductions in the percentage of abnormal sperm were noted in animals treated with PEPM 400 mg/kg. The effects of PEPM on total protein, total cholesterol and testosterone were satisfactory, the levels being increased significantly for protein (P < 0.05), cholesterol (P < 0.01) and testosterone (P < 0.05) by 400 mg/kg PEPM. Microtome sections of the testes of animals treated with 400 mg/kg PEPM exhibited restoration and recovery of germinal cells and the luminal spermatozoa and were comparable with the control group animals. These effects of PEPM make this natural herb ideal as an aphrodisiac and a potent fertility enhancing drug.


Antioxidant And Antiradical Activities Of Phenolic Extracts From Iranian Almond (Prunus Amygdalus L.) Hulls And Shells, Ali Jahanban Isfahlan, Ahmad Mahmoodzadeh, Abdollah Hasanzadeh, Reza Heidari, Rashid Jamei Jan 2010

Antioxidant And Antiradical Activities Of Phenolic Extracts From Iranian Almond (Prunus Amygdalus L.) Hulls And Shells, Ali Jahanban Isfahlan, Ahmad Mahmoodzadeh, Abdollah Hasanzadeh, Reza Heidari, Rashid Jamei

Turkish Journal of Biology

Wild almonds of Iran show genetic diversity and have very a large distribution. Different species of these almonds have more variation in genes resistant to dryness, saltiness, diseases, pests, and other factors. In order to compare the antioxidant and antiradical activity of wild almond hull and shell phenolic extracts, 4 wild almond species (Amygdalus lycioides Spach, A. kotschyi Boiss. & Hohen, A. pabotti Browicz, A. trichamygdalus (Hand.-Mzt) Woronow) were selected from Shahindezh and Qasemloo Valley, West Azerbaijan province, Iran, in 2007. The fruits of these almonds were collected, their hulls and shells were dried and then ground, and then methanolic …


Phytoplankton Composition And Environmental Conditions Of The Mucilage Event In The Sea Of Marmara, Vi̇ldan Tüfekçi̇, Nesli̇han Balkis, Çolpan Polat Beken, Di̇lek Edi̇ger, Mustafa Mantikçi Jan 2010

Phytoplankton Composition And Environmental Conditions Of The Mucilage Event In The Sea Of Marmara, Vi̇ldan Tüfekçi̇, Nesli̇han Balkis, Çolpan Polat Beken, Di̇lek Edi̇ger, Mustafa Mantikçi

Turkish Journal of Biology

The composition and abundance of phytoplankton and the corresponding environmental conditions were investigated during a mucilage event (October 2007-February 2008) in the Sea of Marmara. The mucilage producers Gonyaulax fragilis, Skeletonema costatum, and Cylindrotheca closterium were identified as abundant species. Thallassiosira rotula was also identified in large numbers. The maximum number of G. fragilis was observed at 83,600 cells L^{-1} in November 2007 during the first sampling occasion on the surface layer of İzmit Bay, and T. rotula was the most abundant diatom species, with 131,040 cells L^{-1} in the same period. G. fragilis was recorded at 96,250 cells L^{-1} …


Diurnal Variations Of Summer Phytoplankton And Interactions With Some Physicochemical Characteristics Under Eutrophication In The Dardanelles, Muhammet Türkoğlu, Yasemi̇n Erdoğan Jan 2010

Diurnal Variations Of Summer Phytoplankton And Interactions With Some Physicochemical Characteristics Under Eutrophication In The Dardanelles, Muhammet Türkoğlu, Yasemi̇n Erdoğan

Turkish Journal of Biology

This study was carried out to determine the diurnal distributions of phytoplankton density, bio-volume, and chlorophyll a in relation to nutrients and CTD measurements in surface waters of the coastal area in the Dardanelles during high density of phytoplankton, especially high diatom production (between 3 July and 4 August 2002). Ninety nine surface samples were collected for nutrient, chlorophyll a, and phytoplankton in 3 different time periods of the day (08:00, 13:00 and 18:00 hours) in the sampling period. Based on the sample analyses, average temperature, salinity, pH, DO, TDS, TSS, NO-2+NO-3, PO-34, SiO4, chlorophyll a, N:P, and Si:P were …


Relation Of The Wnt/Β-Catenin Signaling Pathway With Gynecological Cancers, Hani̇fe Güler Tanir, Şayeste Demi̇rezen, Mehmet Si̇nan Beksaç Jan 2010

Relation Of The Wnt/Β-Catenin Signaling Pathway With Gynecological Cancers, Hani̇fe Güler Tanir, Şayeste Demi̇rezen, Mehmet Si̇nan Beksaç

Turkish Journal of Biology

The Wnt signaling pathway is evolutionary conserved and controls many biological processes like cell proliferation and differentiation. It also provides planar polarity, regulation of the cell cycle, and cell adhesion during both the embryonic and adult period. However, it has been widely considered in the literature that some pathological alterations in various biomolecules involved in this pathway, and aberrant activation of signaling have important roles primarily in the genesis of colorectal and cervical cancer and several other serious diseases. The Wnt signaling pathway diversifies into 3 types: Wnt\beta-catenin signaling pathway, planar cell polarity pathway, and Wnt/calcium (Ca^{+2}) pathway. Only the …


The Genotoxic And Antigenotoxic Effects Of Aloe Vera Leaf Extract In Vivo And In Vitro, Ahmet Kayraldiz, Ayşe Yavuz Kocaman, Eyyüp Rencüzoğullari, Erman Sali̇h İsti̇fli̇, Hasan Basri̇ İla, Mehmet Topaktaş, Yusuf Kenan Dağlioğlu Jan 2010

The Genotoxic And Antigenotoxic Effects Of Aloe Vera Leaf Extract In Vivo And In Vitro, Ahmet Kayraldiz, Ayşe Yavuz Kocaman, Eyyüp Rencüzoğullari, Erman Sali̇h İsti̇fli̇, Hasan Basri̇ İla, Mehmet Topaktaş, Yusuf Kenan Dağlioğlu

Turkish Journal of Biology

The genotoxic and antigenotoxic effects of Aloe vera leaf extract (AV) were investigated using the chromosome aberrations (CAs) test for the bone marrow cells of rats, sister chromatid exchanges (SCEs) and micronucleus (MN) and CAs tests for human lymphocytes, and the Ames Salmonella/microsome test system. In the bone marrow cells of rats, AV extract significantly induced structural and total CAs at all concentrations and in all treatment periods. In human peripheral lymphocytes, AV did not increase the mean SCE; however, it significantly induced the MN frequency and structural CAs. In addition, AV showed a cytotoxic effect by decreasing the replication …


Cytogenetic Study On The European Brown Hare (Lepus Europaeus Pallas, 1778) (Mammalia: Lagomorpha) In Turkey, Yasi̇n Demi̇rbaş, Nursel Aşan, İrfan Albayrak Jan 2010

Cytogenetic Study On The European Brown Hare (Lepus Europaeus Pallas, 1778) (Mammalia: Lagomorpha) In Turkey, Yasi̇n Demi̇rbaş, Nursel Aşan, İrfan Albayrak

Turkish Journal of Biology

Conventional and GTC, CTC, and AgNO_3 banded karyotypes of brown hares (Lepus europaeus Pallas, 1778) from Turkey are described for the first time. All specimens possessed a diploid number of 2n = 48 chromosomes and a fundamental autosomal number of NFa = 88. The chromosome set consisted of 8 metacentric and submetacentric pairs and 15 subtelocentric and acrocentric pairs decreasing in size from large to small. The X chromosome was a large submetacentric while the Y was a small acrocentric. In contrast with the other specimens, the Kilis specimen possessed a duplication in the long arm of one of the …


Cytogenetic Effects Of Helichrysum Arenarium In Human Lymphocytes Cultures, Hali̇l Erhan Eroğlu, Ergi̇n Hamzaoğlu, Ahmet Aksoy, Ümi̇t Budak, Sevi̇l Albayrak Jan 2010

Cytogenetic Effects Of Helichrysum Arenarium In Human Lymphocytes Cultures, Hali̇l Erhan Eroğlu, Ergi̇n Hamzaoğlu, Ahmet Aksoy, Ümi̇t Budak, Sevi̇l Albayrak

Turkish Journal of Biology

Helichrysum Mill., belonging to the family Asteraceae, is commonly known as "everlasting". Helichrysum species have been used as herbal tea for centuries in Turkey against gallbladder disorders because of their bile regulatory and diuretic properties. However, there are few reports in the literature regarding the genotoxic effects of these plants. In the present study, the genotoxic effects induced by aqueous (decoction) and methanol extracts of Helichrysum arenarium (L.) Moench subsp. rubicundum (K.Koch) P.H.Davis & Kupicha, H. arenarium (L.) Moench subsp. aucheri (Boiss.) P.H.Davis & Kupicha, and H. arenarium (L.) Moench subsp. erzincanicum P.H.Davis & Kupicha on human lymphocyte cultures at …


Molecular Characterization Of Rhizoctonia Solani Ag4 Using Pcr-Rflp Of The Rdna-Its Region, Meli̇ke Çebi̇ Kiliçoğlu, İbrahi̇m Özkoç Jan 2010

Molecular Characterization Of Rhizoctonia Solani Ag4 Using Pcr-Rflp Of The Rdna-Its Region, Meli̇ke Çebi̇ Kiliçoğlu, İbrahi̇m Özkoç

Turkish Journal of Biology

Rhizoctonia solani isolates obtained from common beans (Phaseolus vulgaris L.) were included in an AG4 anastomosis group in accordance with hyphal anastomosis. In the subgrouping of AG4 isolates, PCR-RFLP patterns in the rDNA-ITS were used. After obtaining the genomic DNA belonging to R. solani AG4, an approximately 700 bp amplification product of the ITS1-5.8S-ITS2 region was obtained with PCR, using ITS1 and ITS4 universal primers. The PCR products were digested with MseI, HincII, AvaII, and MfeI restriction endonucleases, and different PCR-RFLP patterns were obtained for the AG4 HGI and AG4 HGII subgroups. In this study, in addition to the enzymes …


Barley Germplasms Developed For Scald Disease Resistance Exhibited A High Level Of Genetic Diversity Based On Srap Markers, Ayten Di̇zkirici, Zeki̇ Kaya, H. Eli̇f Güren, Hi̇kmet Budak Jan 2010

Barley Germplasms Developed For Scald Disease Resistance Exhibited A High Level Of Genetic Diversity Based On Srap Markers, Ayten Di̇zkirici, Zeki̇ Kaya, H. Eli̇f Güren, Hi̇kmet Budak

Turkish Journal of Biology

The objective of this study was to assess the genetic diversity and genetic relationships among 59 Turkish barley (Hordeum vulgare L.) germplasm lines (maintained for scald disease resistance breeding) using sequence-related amplified polymorphism (SRAP) markers. Seventeen SRAP primer combinations produced 83 polymorphic markers, with a mean polymorphism of 73.5%. The dendrogram created based on Nei's unweighted-pair group method using arithmetic average (UPGMA) indicated that there were 4 main clusters, which was supported by principle component analysis (PCA). Cluster I primarily included only scald-resistant germplasm lines, while clusters III and IV consisted of only scald-sensitive lines, but cluster II had both …


The Effect Of Activator Application On The Anatomy, Morphology, And Viability Of Lycopersicon Esculentum Mill. Pollen, İlkay Öztürk Çali, Feyza Candan Jan 2010

The Effect Of Activator Application On The Anatomy, Morphology, And Viability Of Lycopersicon Esculentum Mill. Pollen, İlkay Öztürk Çali, Feyza Candan

Turkish Journal of Biology

The present study examined the effect of ACT-2, a K-vitamin group activator intensively applied to tomato (Lycopersicon esculentum Mill.), on the anatomy, morphology, and viability of tomato pollen from plants grown under greenhouse conditions. The activator was applied to tomatoes at the recommended dosage (150 cc/100 L of tap water) and double the recommended dosage (300 cc/100 L of tap water). Pollen width and length, exine and intine layer thickness, pore width and length, and crevice width and length were measured using an ocular micrometer. Reductions were observed in all the measured parameters in all application groups, except for pollen …


Physiological And Antioxidant Response Of Three Cultivars Of Cucumber (Cucumis Sativus L.) To Salinity, Gökçen Baysal Furtana, Ruki̇ye Tipirdamaz Jan 2010

Physiological And Antioxidant Response Of Three Cultivars Of Cucumber (Cucumis Sativus L.) To Salinity, Gökçen Baysal Furtana, Ruki̇ye Tipirdamaz

Turkish Journal of Biology

The effects of salinity on the growth, relative water content (RWC), chlorophyll content, inorganic ions, proline accumulation, lipid peroxidation and antioxidant enzymes, superoxide dismutase (SOD), ascorbate peroxidase (APOX), and catalase (CAT) of 3 cucumber (Cucumis sativus L.) cultivars (Çengelköy, Anadolu F1, and Beith Alpha) were investigated. Cucumber cultivars were grown in perlite culture and irrigated with half-strength Hoagland nutrient solution for a period of 35 days under controlled conditions. After this period, seedlings were treated with 150 mM NaCl solution and the plants were harvested on day 7 and day 14 of salt treatment. The fresh and dry weights, RWC, …


Adventitious Shoot Regeneration From Stem Internode Explants Of Verbena Officinalis L., A Medicinal Plant, Arzu Uçar Türker, Buhara Yücesan, Ekrem Gürel Jan 2010

Adventitious Shoot Regeneration From Stem Internode Explants Of Verbena Officinalis L., A Medicinal Plant, Arzu Uçar Türker, Buhara Yücesan, Ekrem Gürel

Turkish Journal of Biology

Verbena officinalis L. (vervain) is a medicinal plant that shows several biological activities, including antimicrobial, antiinflammatory, analgesic, neuroprotective, hypnotic/sedative, gastroprotective, hepatoprotective, anticancer, cicatrizant, and antioxidant. This report describes an efficient plant regeneration system for V. officinalis via adventitious shoot development from stem internode and petiole explants. Stem internode explants were more effective than petiole explants in terms of both the number of shoots per explant and the percentages of explants forming shoots. Increasing benzyladenine (BA) concentrations steadily increased shoot formation up to 13.32 µM, but 22.22 µM BA caused a sharp decrease. The highest number of shoots (17.1 shoots per …