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Articles 1081 - 1110 of 1681
Full-Text Articles in Biology
In Vitro Propagation Of Diyarbakır Watermelons And Comparison Of Direct-Seeded And Transplanted Watermelon, Veysi̇ Okumuş, Vedat Pi̇ri̇nç, Ahmet Onay, Davut Başaran
In Vitro Propagation Of Diyarbakır Watermelons And Comparison Of Direct-Seeded And Transplanted Watermelon, Veysi̇ Okumuş, Vedat Pi̇ri̇nç, Ahmet Onay, Davut Başaran
Turkish Journal of Biology
A rapid protocol using shoot tip explants for micropropagation of Diyarbakır watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] types Sürme, Beyazkış and Karakış was achieved. Shoot tips from 5-day-old in vitro germinated seedlings were cultured on shoot regeneration medium for 3 weeks. The effects of the different concentrations of benzyl adenine (BA) and carbohydrate types on shoot proliferation were examined. The results confirmed that a range of 0.5-1.0 mg/L of BA was almost equally effective in promoting the shoot length of cultures in the 3 genotypes. Sucrose, in the presence of BA, was superior to other carbohydrates for the 3 …
Antioxidant Capacity Of Juice From Different Papaya (Carica Papaya L.) Cultivars Grown Under Greenhouse Conditions In Turkey, Aysun Özkan, Hami̇de Gübbük, Esma Güneş, Ayşe Erdoğan
Antioxidant Capacity Of Juice From Different Papaya (Carica Papaya L.) Cultivars Grown Under Greenhouse Conditions In Turkey, Aysun Özkan, Hami̇de Gübbük, Esma Güneş, Ayşe Erdoğan
Turkish Journal of Biology
The fruits of Carica papaya L. (Caricaceae) are valuable as food and are also used in traditional medicine. The present study was designed to assess the antioxidant potential of the juices of 3 papaya cultivars (PCJ): Sunrise Solo, Red Lady, and Tainung. The antioxidant capacity of PCJ obtained from fully ripened fruit was determined by the following methods: scavenging of the free radical 1,1-diphenyl-2-picrylhydrazyl (DPPH), reducing power assay, scavenging of superoxide radicals, 2-deoxyribose oxidation assay, and thiocyanate assay. Total phenolic content (TPC) of PCJ was determined by the Folin-Ciocalteu method. In the DPPH assay (IC_{50} values), the effect of Sunrise …
Chemical Composition And Nutritional Value Of A Wild Edible Ectomycorrhizal Mushroom, Tricholoma Anatolicum, Erbi̇l Kalmiş, Hasan Yildiz, Bülent Ergönül, Fati̇h Kalyoncu, Mehmet Hali̇l Solak
Chemical Composition And Nutritional Value Of A Wild Edible Ectomycorrhizal Mushroom, Tricholoma Anatolicum, Erbi̇l Kalmiş, Hasan Yildiz, Bülent Ergönül, Fati̇h Kalyoncu, Mehmet Hali̇l Solak
Turkish Journal of Biology
The chemical composition and nutritional value of a wild edible ectomycorrhizal mushroom from southwestern Anatolia, Tricholoma anatolicum, were analyzed. Moisture, crude oil, protein, ash, total carbohydrate content, and mineral composition of the mushrooms studied, including Fe, Na, K, Zn, Cu, Ca, Cd, and Pb, were determined. The energy values of the samples were also calculated. The analyses were conducted during the 3 different growing stages of the mushrooms: mycelium, young fruiting bodies, and mature fruiting bodies. The highest values for moisture and crude oil contents were found to be in the mycelium, ash, and carbohydrate content of young fruiting bodies. …
Biological Characterization Of Iranian Walnut (Juglans Regia) Leaves, Reza Sharafati Chaleshtori, Farhad Sharafati Chaleshtori, Mahmoud Rafieian
Biological Characterization Of Iranian Walnut (Juglans Regia) Leaves, Reza Sharafati Chaleshtori, Farhad Sharafati Chaleshtori, Mahmoud Rafieian
Turkish Journal of Biology
In recent years, emphasis has been placed on the use of natural materials in the control and treatment of various infections, as some chemically synthesized drugs have undesirable side effects. In this study, the ethanol extract of Iranian walnut leaves was examined for antibacterial activities on Streptococcus mutans, Streptococcus salivarius, Streptococcus sanguinis, and Actinomyces viscosus using the microdilution method. Total phenols, flavonoids, and flavonols were also determined colorimetrically. The minimum inhibitory concentrations (MIC) for ethanolic extract ranged between 15.6 and 187.5 mg/mL and minimum bactericidal concentrations (MBC) ranged between 31.25 and 250 mg/mL. Total phenols were 410 ± 14.43 mg/g …
Determination Of Volatile Compounds Of Pine Honeys, Si̇bel Si̇li̇ci̇
Determination Of Volatile Compounds Of Pine Honeys, Si̇bel Si̇li̇ci̇
Turkish Journal of Biology
The volatile compounds of 13 Turkish pine (Pinus brutia Ten.) honey samples were characterized by solid phase microextraction (SPME) analysis, followed by gas chromatography-mass spectrometry (GC-MS) analysis. A total of 42 volatile compounds were identified, but volatile compounds such as nonana1, benzene, 4-hexen-3-ol, alpha-pinene, and 2-heptanone were recognized to be specific floral origin markers of the pine honey. The SPME extraction method was proposed as an alternative way to carry out pollen analysis for floral source detection, especially for products such as pine honey, by characterization of honeydew elements.
The Optimization Of Adna Extraction Protocol And Sex Determination Of Bronze Age Individuals From Oylum Höyük (Kilis, Turkey), Serdal Arslan, Ayşen Açikkol, Ertan Mahi̇r Korkmaz
The Optimization Of Adna Extraction Protocol And Sex Determination Of Bronze Age Individuals From Oylum Höyük (Kilis, Turkey), Serdal Arslan, Ayşen Açikkol, Ertan Mahi̇r Korkmaz
Turkish Journal of Biology
Oylum Höyük falls within the borders of the province of Kilis, located in the southern part of Turkey. It is an important Bronze Age settlement dating back to 5500 BC. In this study, the sex of 36 individuals found in Oylum Höyük was examined using morphological and genetic methods. In the morphological method, sex was identified in 14 female and 13 male individuals. The remaining 8 individuals could not be identified due to the poor preservation of their skeletal remains. Genetic sex was determined with the amplification of sex-specific X, Y centromeric alphoid repeats and ZFX-ZYF gene regions of DNA, …
C- And Nor Stained Karyotypes Of Mole Rat, Nannospalax Xanthodon (2n = 54) From Kırıkkale, Turkey, Ati̇lla Arslan, Kubi̇lay Toyran, Serdar Gözütok, Tarkan Yorulmaz
C- And Nor Stained Karyotypes Of Mole Rat, Nannospalax Xanthodon (2n = 54) From Kırıkkale, Turkey, Ati̇lla Arslan, Kubi̇lay Toyran, Serdar Gözütok, Tarkan Yorulmaz
Turkish Journal of Biology
In the present study, the 2n = 54 chromosomal race of blind mole rats, Nannospalax xanthodon superspecies, from Kırıkkale Province in Turkey was investigated. Conventional chromosome staining, Ag-NOR (Nucleolus Organizer Region) staining, and C-banding analysis were carried out on specimens of mole rats. The karyotype including 3 metacentric pairs (nos. 1-3), 3 submetacentric pairs (nos. 4-6), 3 subtelocentric pairs (nos. 7-9), and 17 acrocentric pairs (nos. 10-26) of autosomes (NFa = 70). C-heterochromatin regions were found in the centromeric and pericentromeric region and the short arms of some bi-armed autosomal pairs, and C-heterochromatin was localized in pericentromeric areas of a …
Cytogenetic And Biochemical Effects Of Imazethapyr In Wheat (Triticum Durum), Manijeh Hoseiny Rad, Ali Ashraf Aivazi, Shobha Jagannath
Cytogenetic And Biochemical Effects Of Imazethapyr In Wheat (Triticum Durum), Manijeh Hoseiny Rad, Ali Ashraf Aivazi, Shobha Jagannath
Turkish Journal of Biology
Mitotic abnormalities, chromosomal behavior, and protein content in wheat (Triticum durum) due to imazethapyr (IM) treatment were determined. Wheat seeds (variety HI-8663) were exposed to 5 IM concentrations (0.5, 0.89, 1.58, 2.81, and 5 ppm) according to the between paper method, in laboratory conditions. The protein content in the root-shoot axis and endosperm decreased significantly with the dosage increase. Cytological observations showed that the mitotic frequency in the root meristematic cells decreased parallel to the increase in concentrations. The most common types of the observed anomalies were sticky chromosomes, lagging chromosomes, scattered chromosomes, chromatin bridges, and micronuclei. The results revealed …
Studies On The Effect Of Ph And Carbon Sources On Enzyme Activities Of Some Pectinolytic Bacteria Isolated From Jute Retting Water, Biswapriya Das, Ashis Chakraborty, Sagarmoy Ghosh, Kalyan Chakrabarti
Studies On The Effect Of Ph And Carbon Sources On Enzyme Activities Of Some Pectinolytic Bacteria Isolated From Jute Retting Water, Biswapriya Das, Ashis Chakraborty, Sagarmoy Ghosh, Kalyan Chakrabarti
Turkish Journal of Biology
The effect of pH of the assay media and different carbon sources in the culture media on the activities of 4 different enzymes, namely polygalacturonase (PG), pectin lyase (PNL), xylanase, and cellulase, of some pectinolytic bacteria were studied. The pectinolytic bacterial strains were isolated from the jute retting water bodies of 2 important jute growing areas of North 24 Parganas, West Bengal, India. The highest PG (12.45 to 41.0 IU/g cell wet weight) and PNL (22.55 to 75.7 U/mL) activities occurred at the alkaline pH of 8.0 and 8.5, respectively. Maximum xylanase activity (0.01 to 0.735 \mumol/mL/min) occurred at pH …
Detection Of Prokaryotic Microbial Communities Of Çamaltı Saltern, Turkey, By Fluorescein In Situ Hybridization And Real-Time Pcr, Mehmet Burçi̇n Mutlu, Kiymet Güven
Detection Of Prokaryotic Microbial Communities Of Çamaltı Saltern, Turkey, By Fluorescein In Situ Hybridization And Real-Time Pcr, Mehmet Burçi̇n Mutlu, Kiymet Güven
Turkish Journal of Biology
Knowledge of the functions, interactions, and diversity of extremely halophilic microorganisms mostly comes from the results of studies performed in different salterns throughout the world. As model habitats, salterns allow the comparison of different techniques used for qualitative and quantitative analysis of halophilic communities in these hypersaline environments. Çamaltı Saltern is the biggest coastal solar saltern located on the Aegean coast of Turkey, and it produces most of the salt consumed in the country. In the present study, detection of prokaryotic communities of the Çamaltı Saltern was performed using 2 culture-independent methods. Real-time polymerase chain reaction (RT-PCR) and fluorescein in …
Production Of A Novel Bifunctional Catalase-Phenol Oxidase Of Scytalidium Thermophilum In The Presence Of Phenolic Compounds, Yonca Yüzügüllü, Zümrüt Begüm Ögel, Ufuk Bölükbaşi, Nursen Çoruh, Gürkan Karakaş
Production Of A Novel Bifunctional Catalase-Phenol Oxidase Of Scytalidium Thermophilum In The Presence Of Phenolic Compounds, Yonca Yüzügüllü, Zümrüt Begüm Ögel, Ufuk Bölükbaşi, Nursen Çoruh, Gürkan Karakaş
Turkish Journal of Biology
A novel bifunctional catalase with additional phenol oxidase activity (CATPO) is produced by the thermophilic fungus, Scytalidium thermophilum, in a growth-associated manner. In order to study the biological significance of this dual enzyme in relation to phenolic compounds, 14 phenolics were tested for their effect on growth and CATPO production. It was determined that some phenolics exerted a negative effect on growth (catechol, coumaric acid, hydroquinone, kaempferol, myricetin), while others either did not influence growth in a negative manner or enhanced growth by a maximum of 50% increase in biomass (caffeic acid, chlorogenic acid, catechin, gallic acid, resorcinol, vanillic acid). …
Production, Purification, And Characterization Of \Alpha-Amylase By Bacillus Subtilis And Its Mutant Derivates, Eli̇f Demi̇rkan
Production, Purification, And Characterization Of \Alpha-Amylase By Bacillus Subtilis And Its Mutant Derivates, Eli̇f Demi̇rkan
Turkish Journal of Biology
The effects of various carbon and nitrogen sources on production of \alpha-amylase by Bacillus subtilis and its mutant derivates were investigated. The maximum production of \alpha-amylase by all strains was obtained in the presence of mesoinositol as the carbon source. There was no more significant increase in enzyme yield in the case of the supplementation of nitrogen sources, whereas malt extract and tryptone were preferred nitrogen sources for amylase production by Bacillus subtilis and mutant U 2-6 strain, respectively. \alpha-Amylases of B. subtilis and its mutant strain (EBUE 5-3) were purified through a series of steps, and characterized. The optimum …
A Comparative Evaluation Of Antioxidant And Anticancer Activity Of Essential Oil From Origanum Onites (Lamiaceae) And Its Two Major Phenolic Components, Aysun Özkan, Ayşe Erdoğan
A Comparative Evaluation Of Antioxidant And Anticancer Activity Of Essential Oil From Origanum Onites (Lamiaceae) And Its Two Major Phenolic Components, Aysun Özkan, Ayşe Erdoğan
Turkish Journal of Biology
The objectives of the present study were to determine the effects of essential oil from Turkish oregano (Origanum onites L.) and its 2 major phenolic components, carvacrol and thymol, on cell viability, and to investigate their cytoprotective (antioxidant) effects against hydrogen peroxide-induced cytotoxicity and membrane damage in hepatoma G2 (Hep G2) cells. Their antioxidant properties were also evaluated with 2 in vitro complementary test systems: DPPH radical scavenging activity and linoleic acid oxidation inhibition. The aerial parts of the essential oil were isolated using a Clevenger-type apparatus (hydrodistillation) and its components were analyzed by gas chromatography/mass spectrometry (GC/MS). The essential …
Indirect Somatic Embryogenesis And Shoot Organogenesis From Cotyledonary Leaf Segments Of Digitalis Lamarckii Ivan., An Endemic Medicinal Species, Sandeep K. Verma, Bahti̇yar Buhara Yücesan, Songül Gürel, Ekrem Gürel
Indirect Somatic Embryogenesis And Shoot Organogenesis From Cotyledonary Leaf Segments Of Digitalis Lamarckii Ivan., An Endemic Medicinal Species, Sandeep K. Verma, Bahti̇yar Buhara Yücesan, Songül Gürel, Ekrem Gürel
Turkish Journal of Biology
This study describes, for the first time, an in vitro protocol for the development of somatic embryos and shoots from a callus derived from cotyledonary leaf segments excised from 3-week-old in vitro-germinated seedlings of Digitalis lamarckii Ivan. (dwarf foxglove), an endemic medicinal species of Turkey. The embryogenic callus was induced on Murashige and Skoog (MS) medium containing 0.54 \muM \mu-naphthalene acetic acid (NAA) and 2.22 \muM 6-benzylaminopurine (BAP). Somatic embryos developed readily when the embryogenic callus was transferred to MS medium containing BAP (4.44 or 8.87 \muM) alone or BAP combined with NAA (1.34, 2.69, or 5.37 \muM). The most …
Bioassay Studies Of 2'-O-Ethylmurrangatin Isolated From A Medicinal Plant, Murraya Paniculata, Azizuddin Shaikh, Muhammad Iqbal Choudhary
Bioassay Studies Of 2'-O-Ethylmurrangatin Isolated From A Medicinal Plant, Murraya Paniculata, Azizuddin Shaikh, Muhammad Iqbal Choudhary
Turkish Journal of Biology
A secondary metabolite, 2^'-O-ethylmurrangatin (1), was isolated from the leaves of a medicinal plant, Murraya paniculata. The structure of compound 1 was identified with the help of spectroscopic techniques. Compound 1 was screened for its antioxidant, lipoxygenase, prolyl endopeptidase, and respiratory burst inhibitory activities. 2^'-O-Ethylmurrangatin (1) was found to have significant activity against lipoxygenase enzyme and moderate respiratory burst inhibitory activity, but no activity against either DPPH radical scavenging assay or prolyl endopeptidase enzyme.
Anti-Inflammatory Activity Of Anthemis Aciphylla Var. Aciphylla Boiss., Si̇nem Özkaynak, Hati̇ce Efsun Kolatan, Osman Yilmaz, Bi̇jen Kivçak
Anti-Inflammatory Activity Of Anthemis Aciphylla Var. Aciphylla Boiss., Si̇nem Özkaynak, Hati̇ce Efsun Kolatan, Osman Yilmaz, Bi̇jen Kivçak
Turkish Journal of Biology
Ethanolic (EEAA) and sesquiterpene lactone (SEAA) extracts of the aerial parts of Anthemis aciphylla var. aciphylla Boiss. (Asteraceae) were evaluated for anti-inflammatory activity on carrageenin-induced paw edema (acute model) and cotton pellet-induced granuloma (chronic model) in rats. In carrageenin-induced paw edema, EEAA and SEAA at intraperitoneal doses of 50, 100, and 200 mg/kg dose-dependently inhibited the paw edema. In cotton pellet-induced granuloma, the oral administration of EEAA and SEAA at 50, 100, and 200 mg/kg dosages was also found to significantly inhibit granuloma tissue formation.
Evaluation Of The Antioxidant And Acetylcholinesterase Inhibitory Activities Of Arnebia Densiflora Ledeb., İlkay Orhan, F. Sezer Şenol, Ufuk Koca, Tuğba Erçeti̇n, Gülnur Toker
Evaluation Of The Antioxidant And Acetylcholinesterase Inhibitory Activities Of Arnebia Densiflora Ledeb., İlkay Orhan, F. Sezer Şenol, Ufuk Koca, Tuğba Erçeti̇n, Gülnur Toker
Turkish Journal of Biology
Arnebia densiflora Ledeb. (Boraginaceae), a medicinal plant growing in Turkey, has been reported to contain a well-known red pigment, shikonin, a naphthoquinone derivative. In the current study, the acetylcholinesterase (AChE) inhibitory and antioxidant activities of the chloroform, ethyl acetate, methanol, and water extracts of the root, stem, and flowers of the plant, as well as shikonin, were investigated. AChE inhibition was tested using an ELISA microplate reader at 250, 500, 1000, and 2000 µg mL^{-1}. Antioxidant activity was determined by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging test and the Fe+2-ferrozine test system for metal chelating power. The results indicated that the …
Chromosome Aberrations Induced By Curcumin And Aloin In Allium Cepa L. Root Meristem Cells, Loganathan Palanikumar, Irulappan Ragunathan, Natarajan Panneerselvam
Chromosome Aberrations Induced By Curcumin And Aloin In Allium Cepa L. Root Meristem Cells, Loganathan Palanikumar, Irulappan Ragunathan, Natarajan Panneerselvam
Turkish Journal of Biology
To date, a variety of plant compounds have been studied extensively with regard to anti-mutagenic, anti-carcinogenic, and anti-genotoxic activity. Nevertheless, very few of them have been studied in terms of clastogenicity. In the present study the clastogenic activity of curcumin (the active component of turmeric) and aloin (the active component of aloe) was evaluated at doses of 5, 10, 20, 30, 50, and 100 µg/mL for 4, 8, and 12 h. Both curcumin and aloin had significant dose- and time-dependent clastogenic effects on the test plant system (Allium cepa L.). Compared to curcumin, aloin was more clastogenic. Clastogenic activity was …
Isolation And Identification Of Trichoderma Species From Different Habitats And Their Use For Bioconversion Of Solid Waste, Ahsanur Rahman, Most. Ferdousi Begum, Matiur Rahman, M. A. Bari, G.N.M. Illias, M. Firoz Alam
Isolation And Identification Of Trichoderma Species From Different Habitats And Their Use For Bioconversion Of Solid Waste, Ahsanur Rahman, Most. Ferdousi Begum, Matiur Rahman, M. A. Bari, G.N.M. Illias, M. Firoz Alam
Turkish Journal of Biology
The native population of Trichoderma species collected from different habitats was quantified and characterised. The identified strains are T. harzianum (IMI-392432, 392433, 392434); T. pseudokoningii (IMI-392431) and T. virens (IMI-392430). Out of five strains, T. harzianum was the most common in all of the habitats. Colony forming units (cfu's) of Trichoderma species varied significantly (P = 0.05) in different habitats and were positively correlated with the physico-chemical characteristics of the habitat. Trichoderma was found to have adapted to an acidic habitat. In testing for an efficient bioconversion agent, T. harzianum (IMI-392432) was found to be the most effective in kitchen …
The Antioxidant And Dna Protection Potential Of Indian Tribal Medicinal Plants, Gunjan Guha, Venkatadri Rajkumar, Lazar Mathew, Rangasamy Ashok Kumar
The Antioxidant And Dna Protection Potential Of Indian Tribal Medicinal Plants, Gunjan Guha, Venkatadri Rajkumar, Lazar Mathew, Rangasamy Ashok Kumar
Turkish Journal of Biology
Prevention and treatment of various degenerative diseases using traditional medicines is increasingly generating interest, especially in geriatric clinical research. This study aimed to evaluate the free-radical scavenging properties and potential to prevent DNA damage (from oxidative stress) of 56 extracts (polar and non-polar) from 14 medicinal plants (from 12 families) used by diverse Indian tribes and ethnic groups. Although widely known for traditional uses, these plants have not been extensively used for clinical purposes. Scavenging of DPPH^{\bullet} radical by all the extracts was evaluated to estimate their antioxidant potential and rank them accordingly. Extracts occupying the top 10 ranks were …
Spectrum And Frequency Of Macromutations Induced In Chickpea (Cicer Arietinum L.), Aijaz A. Wani
Spectrum And Frequency Of Macromutations Induced In Chickpea (Cicer Arietinum L.), Aijaz A. Wani
Turkish Journal of Biology
The present investigation was undertaken to study the frequency and spectrum of morphological mutants induced by gamma rays and EMS in chickpea (Cicer arietinum L.). The seeds of 2 varieties of chickpea, Pusa-212 and Pusa-372, were treated with gamma rays (150 Gy, 200 Gy, 300 Gy, and 400 Gy), EMS (0.1%, 0.2%, 0.3%, and 0.4%) and combination treatments (200Gy+0.2%EMS, 300Gy+0.2%EMS, 200Gy+0.3%EMS, and 300 Gy+0.3%EMS). The M2 population was carefully screened for various viable macromutations. A wide spectrum of viable morphological mutations affecting almost all parts of the plant were isolated in the M2 generation. The most striking mutants isolated included …
Total Phenolic Contents And Antioxidant Activities Of Prangos Lindl. (Umbelliferae) Species Growing In Konya Province (Turkey), Jehad Ahmed, Ayşegül Güvenç, Nurgün Küçükboyaci, Ayşe Baldemi̇r, Maksut Coşkun
Total Phenolic Contents And Antioxidant Activities Of Prangos Lindl. (Umbelliferae) Species Growing In Konya Province (Turkey), Jehad Ahmed, Ayşegül Güvenç, Nurgün Küçükboyaci, Ayşe Baldemi̇r, Maksut Coşkun
Turkish Journal of Biology
Prangos species are widely used as medicinal plants in Turkey, and 14 species of this genus grow naturally in Anatolia. In the present study, the phenolic contents and antioxidant activities of the water and methanol (MeOH) extracts obtained from the root, herb, and fruits of 4 species of Prangos (Prangos ferulacea, P. heyniae, P. meliocarpoides var. meliocarpoides, and P. uechtritzii) collected in Konya province were compared. The phenolic contents of the samples were determined using Folin-Ciocalteu's phenol reagent. Antioxidant activities of the extracts were studied by qualitative DPPH^. (1,1-diphenyl-2-picrylhydrazyl radical) assay to detect the free radical scavenging activity and by …
Molecular Cloning, Characterization, And Expression Analysis Of A Gene Encoding A Ran Binding Protein (Ranbp) In Cucumis Melo L., Mehmet Cengi̇z Baloğlu, Florence Negre-Zakharov, Hüseyi̇n Avni̇ Öktem, Ayşe Meral Yücel
Molecular Cloning, Characterization, And Expression Analysis Of A Gene Encoding A Ran Binding Protein (Ranbp) In Cucumis Melo L., Mehmet Cengi̇z Baloğlu, Florence Negre-Zakharov, Hüseyi̇n Avni̇ Öktem, Ayşe Meral Yücel
Turkish Journal of Biology
Ran binding proteins (RanBPs) are highly conserved members of the GTP-binding protein family that are involved in nuclear protein export between the nucleus and the cytoplasm. In this study, a CmRanBP gene from a melon was isolated (Cucumis melo L.) using the RACE (rapid amplification of cDNA ends) method. The 778 basepair long melon, with a RanBP cDNA encoding consisting of 197 amino acids (22.2 kDa protein), was characterized (GenBank accession no: EU853459). The predicted amino acid sequence of CmRanBP was found to be 70% identical to VvRanBP, PtRanBP, and RcRanBP from Vitis vinifera, Populus trichocarpa, and Ricinus communis, respectively. …
Biochemical Response Of Olea Europaea Cv. Gemlik To Short-Term Salt Stress, Mehmet Ali̇ Demi̇ral, Deni̇z Aktaş Uygun, Murat Uygun, Erkan Kasirğa, Ari̇fe Alev Karagözler
Biochemical Response Of Olea Europaea Cv. Gemlik To Short-Term Salt Stress, Mehmet Ali̇ Demi̇ral, Deni̇z Aktaş Uygun, Murat Uygun, Erkan Kasirğa, Ari̇fe Alev Karagözler
Turkish Journal of Biology
One-year-old olive (Olea europaea L. cv. Gemlik) seedlings were exposed to increasing levels of salinity in pot culture. The experiment was laid out in a randomized block design with 6 replicates. Dry matter (DM) percentage, sodium (Na) and chloride (Cl) concentrations, 1,1-diphenyl-2-picryl hydrazyl (DPPH) scavenging activity, reducing power (RP), total phenolic content (TPC), and proline (PRO) content were determined in the leaves of the plants. The results showed that the olive plant used physiological and biochemical mechanisms consecutively to alleviate the effects of salt stress. The type of mechanism used was related to the severity of the salt stress. Salinity-induced …
Biochemical Identification And Numerical Taxonomy Of Aeromonas Spp. Isolated From Food Samples In Turkey, Belgi̇n Erdem, Ergi̇n Kari̇ptaş, Eli̇f Çi̇l, Kami̇l Işik
Biochemical Identification And Numerical Taxonomy Of Aeromonas Spp. Isolated From Food Samples In Turkey, Belgi̇n Erdem, Ergi̇n Kari̇ptaş, Eli̇f Çi̇l, Kami̇l Işik
Turkish Journal of Biology
Numerical taxonomy was used to analyze phenotypic data obtained from 126 new isolates of Aeromonas strains taken from red meat, raw chicken, minced meat, and fish samples. Each strain was tested for 86 characters but only the final data including 63 characters were analysed using the S_{SM} coefficients and the UPGMA clustering algorithm. At S_{SM} values of => 83%, the strains clustered into 10 aggregate groups consisting of 7 major (5 and up strains) and 3 minor (2-4 strains), and 5 single member clusters, each of which was identified as A. hydrophila, A. caviae, and A. sobria, respectively. It was …
Numerical Classification Of Streptomycetes Isolated From Karstic Caves In Turkey, Mustafa Yamaç, Kami̇l Işik, Nevzat Şahi̇n
Numerical Classification Of Streptomycetes Isolated From Karstic Caves In Turkey, Mustafa Yamaç, Kami̇l Işik, Nevzat Şahi̇n
Turkish Journal of Biology
In the present study, a total of 290 actinomycetes isolates were obtained from 19 karstic caves in Turkey. Out of these, 131 were chosen for numerical classification studies according to their morphology and pigmentation properties. Representative isolates were compared with the reference Streptomyces strains based on their various taxonomic characteristics such as biochemical, physiological, nutritional, and morphological, totalling 134 unit characters. The data were evaluated using simple matching coefficients (S_{SM}), and clustering achieved using the Complete algorithm by X-Taxon and NTSys-pc programs. The test strains were assigned to 9 major (5-11 strains), 18 minor (2-4 strains), and 31 single membered …
Preliminary Studies On Antiinflammatory, Antipyretic, And Antidiarrhoeal Properties Of Evolvulus Alsinoides, U.M.Dhana Lekshmi, P.Neelakanta Reddy
Preliminary Studies On Antiinflammatory, Antipyretic, And Antidiarrhoeal Properties Of Evolvulus Alsinoides, U.M.Dhana Lekshmi, P.Neelakanta Reddy
Turkish Journal of Biology
The ethanolic extract of the whole plant of Evolvulus alsinoides L. (Convolvulaceae) was investigated for antiinflammatory, antipyretic and antidiarrhoeal properties in female albino rats. Preliminary phytochemical studies were carried out to confirm the presence of active chemical constituents in the plant extract, and the result showed a positive report for the presence of flavonoids, alkaloids and cardiac glycosides. The pharmacological result showed that the ethanolic extract at a dose of 250 mg/kg body weight and 500 mg/kg body weight caused a significant (P < 0.05) inhibition of the carrageenan-induced rat paw oedema and a significant (P < 0.05) reduction of hyperpyrexia induced by Brewer's yeast in rats. The extract also elicited marked antidiarrhoeal activity against castor oil-induced diarrhoea at a dose of 500 mg/kg. This study has proved scientifically the ancient claim that the whole plant of Evolvulus alsinoides has antiinflammatory, antipyretic and antidiarrhoeal activities.
Investigation Of Legionella Pneumophila And Free Living Amoebas In The Domestic Hot Water Systems In İstanbul, Di̇lara Meryem Burak, Zuhal Zeybek
Investigation Of Legionella Pneumophila And Free Living Amoebas In The Domestic Hot Water Systems In İstanbul, Di̇lara Meryem Burak, Zuhal Zeybek
Turkish Journal of Biology
Legionella pneumophila (L. pneumophila), known to cause legionnaires' disease, has been found in various aquatic environments and man-made water systems. Free living amoebas (FLAs) live in the same environments and can cause infections such as encephalitis, keratitis. Moreover, FLAs play a major role in L. pneumophila transmission. In the present study, the presence of L. pneumophila and FLAs was examined in water and swab samples obtained from shower heads (hot water) in 61 houses in İstanbul. Nonnutrient agar (NNA) was used for isolation of FLAs in all samples and buffered charcoal yeast extract (BCYE) agar with addition of glycine, vancomycine, …
Utilization Of Cytochrome Oxidase I In Cephus Pygmeus (L.) (Hymenoptera: Cephidae), Ertan Mahi̇r Korkmaz, Mahi̇r Budak, Hasan Hüseyi̇n Başibüyük
Utilization Of Cytochrome Oxidase I In Cephus Pygmeus (L.) (Hymenoptera: Cephidae), Ertan Mahi̇r Korkmaz, Mahi̇r Budak, Hasan Hüseyi̇n Başibüyük
Turkish Journal of Biology
The mitochondrial cytochrome oxidase I (COI) gene region of Cephus pygmeus (L.) (Hymenoptera: Cephidae) was characterized at the nucleotide and amino acid levels. The partial sequence (687 bp) of the gene was determined and compared with other cephids and insect species at both the nucleic acid and amino acid sequence levels. The examined fragment was placed at an equal distance to both ends of the gene and consisted of 13 structural regions. The 5' end of the partial COI gene was more informative than the 3' end at the intraspecific level. Pairwise sequence divergences ranged from 0.15% to 1.17% at …
The Effects Of Leptin Hormone On Locomotor Activity In Syrian Hamsters (Mesocricetus Auratus), Bülent Gündüz, Alper Karakaş
The Effects Of Leptin Hormone On Locomotor Activity In Syrian Hamsters (Mesocricetus Auratus), Bülent Gündüz, Alper Karakaş
Turkish Journal of Biology
The suprachiasmatic nucleus (SCN) generates and controls the circadian rhythms in mammals including the rhythm of locomotor activity. Leptin is a hormone secreted by adipose tissue that informs the brain about the fat stores. SCN neurons express leptin receptors. Here we investigated the effects of 3 different leptin administrations on the locomotor activity of the Syrian hamsters maintained in constant darkness. Animals were intraperitoneally (ip) injected (4 µg/kg), subcutaneously (sc) infused (4 µg/kg), or intra-SCN infused (0.4 µg/kg) with leptin for 3 days at circadian time 10 whereas the controls received saline (0.9% NaCl) at the same time in order …