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Articles 1051 - 1080 of 1681
Full-Text Articles in Biology
Effects Of Plant Growth Promoting Rhizobacteria On Fruit Set, Pomological And Chemical Characteristics, Color Values, And Vegetative Growth Of Sour Cherry (Prunus Cerasus Cv. Kütahya), Hali̇l Karakurt, Recep Kotan, Fati̇h Dadaşoğlu, Rafet Aslantaş, Fi̇kretti̇n Şahi̇n
Effects Of Plant Growth Promoting Rhizobacteria On Fruit Set, Pomological And Chemical Characteristics, Color Values, And Vegetative Growth Of Sour Cherry (Prunus Cerasus Cv. Kütahya), Hali̇l Karakurt, Recep Kotan, Fati̇h Dadaşoğlu, Rafet Aslantaş, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
This study was performed to determine effects of 4 plant growth promoting rhizobacteria (Bacillus subtilis OSU - 142, Bacillus megaterium M - 3, Burkholderia cepacia OSU - 7, and Pseudomonas putida BA - 8) alone and in combinations on fruit set of sour cherry trees (Prunus cerasus L., cv. Kütahya), and to investigate their resulting pomological and chemical characteristics as well as vegetative growth. All the tested bacterial strains alone or some of their combinations have a great potential to increase especially fruit set and plant vegetative growth, and indirectly affect fruit pomological and chemical characteristics. Therefore, they may be …
The Protective Effect Of Boric Acid On Aluminum-Induced Hepatotoxicity And Genotoxicity In Rats, Hasan Türkez, Fati̇me Geyi̇koğlu, Suat Çolak
The Protective Effect Of Boric Acid On Aluminum-Induced Hepatotoxicity And Genotoxicity In Rats, Hasan Türkez, Fati̇me Geyi̇koğlu, Suat Çolak
Turkish Journal of Biology
The efficacy of boric acid (BA) was examined on liver marker enzymes in aluminum (Al)-treated rats. Also, a liver micronucleus assay was performed to evaluate the genotoxicity in hepatocytes. With these aims, Sprague-Dawley rats were randomly separated into 10 groups of 5 animals. Aluminum chloride (5 mg/kg body weight (b.w.) AlCl3) and BA (3.25, 13, 36 and 58.5 mg/kg b.w.) alone were administered with injections to the experimental animals. Furthermore, the animals were also treated with Al for 4 consecutive days followed by BA exposure for 10 days. The rats were anesthetized after Al and BA injections and the levels …
Antagonistic Potential Of Marine Actinobacteria Against Fish And Shellfish Pathogens, Selvakumar Dharmaraj
Antagonistic Potential Of Marine Actinobacteria Against Fish And Shellfish Pathogens, Selvakumar Dharmaraj
Turkish Journal of Biology
A total of 94 marine actinobacterial isolates were found to be associated with 4 sponges: Callyspongia diffusa, Mycale mytilorum, Tedania anhelans, and Dysidea fragilis. Among the cultures 7 strains showed antagonism against fish and shellfish pathogens. Analysis of morphological, physiological, biochemical, and protein profile characteristics suggested that the actinobacterial strains belonged to the genus Streptomyces. The initial screening of the isolates by the spot inoculation method exhibited antibacterial activity against Aeromonas hydrophila. In vitro screening of the submerged culture extracts showed positive inhibition against the fish and shellfish pathogens Aeromonas hydrophila, Serratia sp., and Vibrio spp. The screening of bioactive …
Isolation And Identification Of Lactic Acid Bacteria From Boza, And Their Microbial Activity Against Several Reporter Strains, Meri̇h Kivanç, Meral Yilmaz, Erdoğan Çakir
Isolation And Identification Of Lactic Acid Bacteria From Boza, And Their Microbial Activity Against Several Reporter Strains, Meri̇h Kivanç, Meral Yilmaz, Erdoğan Çakir
Turkish Journal of Biology
A total of 45 lactic acid bacteria were isolated from 10 different boza samples consumed in Turkey. The isolates were identified with respect to colonial, cellular morphology, and biochemical characters based on API CHL50 (Biomérieux) kit and also automated riboprinting was used to identify of the isolates. These isolates were tested for inhibitory activity against food-borne bacterial pathogens. Antimicrobial effects of these lactic acid bacteria were determined by the agar spot test and also agar diffusion method. As a result of the RiboPrinter® Microbial Characterization System (DuPont Qualicon) lactic acid bacteria isolates having antimicrobial activity (45 isolates) were identified as …
Determining Mitotic Index In Peripheral Lymphocytes Of Welders Exposed To Metal Arc Welding Fumes, Ceylan Alakoç, Hali̇l Erhan Eroğlu
Determining Mitotic Index In Peripheral Lymphocytes Of Welders Exposed To Metal Arc Welding Fumes, Ceylan Alakoç, Hali̇l Erhan Eroğlu
Turkish Journal of Biology
Gas metal arc welding is a widely used method in a lot of industrial areas as it is cheap and produces high quality results. In the present study, peripheral blood lymphocytes of 23 welders and 25 non-exposed subjects were monitored for cytotoxicity. Information regarding the subjects' ages, smoking habits, alcohol consumption, duration of exposure, and medicine usage was recorded. According to the results, mitotic index (2.278 ± 1.325) of the welders was higher than that of those non-exposed (0.956 ± 0.616) (P < 0.01). There was no significant difference between mitotic index rates of smoking and non-smoking subjects. In contrast, there were significant differences between non-exposed subjects and welders for both smoking and non-smoking (P < 0.05). It was found that as the time of exposure to welding fumes increased so did the mitotic index rates (P < 0.05). Both a positive correlation in welders and a negative correlation in non-exposed subjects were observed between mitotic index frequency and age. In the light of the results obtained, it is advised that welders who work with gas metal arc welding be instructed and necessary protective measures be taken because of the risk caused by metal arc welding fumes.
Comparative Analysis Of Dna Extracted From Fish Species Preserved In Formalin In Two Different Periods, Manickam Raja, Murugan Muralidharan, Muthukumarasamy Arunachalam
Comparative Analysis Of Dna Extracted From Fish Species Preserved In Formalin In Two Different Periods, Manickam Raja, Murugan Muralidharan, Muthukumarasamy Arunachalam
Turkish Journal of Biology
DNA was extracted from 2 cyprinid fishes kept under formalin preservation in 2 different time durations, and the quality and quantity were analyzed. The tissue samples from specimens preserved for shorter duration yielded higher quantity of DNA compared to long-term preserved tissues. ANOVA (one way) on the quantity of the DNA from the samples of 2 periods showed that the genetic material obtained from the recently preserved samples was highly significant (P > 0.001). The quality of the genetic material irrespective of the period of preservation was found to be the same. The purpose of the study was to check the …
Electrophoretic Analysis Of Genetic Diversity In Natural House Fly (Musca Domestica L.) Populations From The Western And Southern Coasts Of Turkey, Belgi̇n Göçmen Taşkin, Vatan Taşkin, Köksal Küçükakyüz
Electrophoretic Analysis Of Genetic Diversity In Natural House Fly (Musca Domestica L.) Populations From The Western And Southern Coasts Of Turkey, Belgi̇n Göçmen Taşkin, Vatan Taşkin, Köksal Küçükakyüz
Turkish Journal of Biology
In this study genetic variation was determined with isozyme analysis in 8 natural house fly (Musca domestica L.) populations from the western and southern coasts of Turkey, a part of the southern Palearctic region. For this purpose, 7 isozyme systems (ACP, AOX, CAT, HBDH, HXK, MDH, and SOD) were resolved using the native polyacrylamide gel electrophoresis (native-PAGE) technique. A total of 9 loci and 20 alleles were detected. The proportion of polymorphic loci in populations ranged from 44.44% to 66.67%. The overall mean number of effective alleles per locus (Ne) was 1.314 ± 0.454. The mean estimated expected heterozygosity (He) …
The Analgesic Effect Of The Ethanolic Extract Of Matricaria Aurea, Esam Qnais
The Analgesic Effect Of The Ethanolic Extract Of Matricaria Aurea, Esam Qnais
Turkish Journal of Biology
The analgesic effect of the ethanolic whole plant extract of Matricaria aurea L. (Asteraceae) was studied in rats using the cold water tail flick assay and in mice using the tail immersion, tail clip, acetic-acid-induced writhing, and formalin pain tests. The results showed dose-dependent and significant (P < 0.001) increases in pain threshold at 60 min post-treatment with doses of 100, 200, and 300 mg/kg of the extract in tail flick, tail immersion, and tail clip tests. The effects of the extract were significantly (P < 0.001) lower than those produced by morphine (10 mg/kg) in the same tests. The extract (100, 200, and 300 mg/kg) exhibited a dose-dependent inhibition of writhing and also showed a significant (P < 0.001) inhibition in both phases of the formalin pain test, but with a less intense effect in the first phase. The results indicate that the analgesic effect of M. aurea ethanolic extract is both centrally and peripherally mediated.
Determination Of Antimicrobial And Antiproliferative Activities Of The Aquatic Moss Fontinalis Antipyretica Hedw., Fi̇li̇z Savaroğlu, Cansu Fi̇li̇k İşçen, Ayşe Pinar Öztopcu Vatan, Selda Kabadere, Semra İlhan, Ruhi̇ Uyar
Determination Of Antimicrobial And Antiproliferative Activities Of The Aquatic Moss Fontinalis Antipyretica Hedw., Fi̇li̇z Savaroğlu, Cansu Fi̇li̇k İşçen, Ayşe Pinar Öztopcu Vatan, Selda Kabadere, Semra İlhan, Ruhi̇ Uyar
Turkish Journal of Biology
The purpose of the current study was to investigate the possible antimicrobial and anticancer properties of the aquatic moss Fontinalis antipyretica Hedw. (Fontinalaceae). We first obtained 8 different extracts (methanol; chloroform; acetone; ethyl acetate A, B, C, and D) by two different extraction processes. The antimicrobial activity of these extracts was then assessed by using the well diffusion method against 8 bacterial and 7 fungal strains. The results of antimicrobial studies showed that extracts of chloroform, acetone, and ethyl acetate A and C were active against almost all the species tested. The most effective extract was C, which was then …
Levels Of Phenolic Compounds And Their Effects On Antioxidant Capacity Of Wild Vaccinium Arctostaphylos L. (Qare-Qat) Collected From Different Regions Of Iran, Tahereh Hasanloo, Roshanak Sepehrifar, Homa Hajimehdipoor
Levels Of Phenolic Compounds And Their Effects On Antioxidant Capacity Of Wild Vaccinium Arctostaphylos L. (Qare-Qat) Collected From Different Regions Of Iran, Tahereh Hasanloo, Roshanak Sepehrifar, Homa Hajimehdipoor
Turkish Journal of Biology
Antioxidant activities in the leaves and fruits of wild Vaccinium arctostaphylos (locally named Qare-Qat) from different regions of Iran were evaluated using the stable 1, 1-diphenyl-2-picrylhydrazyl free radical and ferric reducing/antioxidant power methods. Variations in antioxidant activity, total phenolic, anthocyanin, and flavonoid content in fruit samples among genotypes was much greater than the variation observed between growing seasons, indicating that genetics plays a more important role than growing season in V. arctostaphylos fruits. The data indicated that the fruits of the Kelardasht genotype had the highest total phenolic content [42.73 ± 1.5 mg gallic acid equivalents (GAE) g^{-1} DW], anthocyanin …
Characterisation And Antimicrobial Activity Of Sophora Alopecuroides L. Var. Alopecuroides Alkaloid Extracts, Nurgün Küçükboyaci, Semi̇ha Özkan, Nezaket Adigüzel, Fatma Tosun
Characterisation And Antimicrobial Activity Of Sophora Alopecuroides L. Var. Alopecuroides Alkaloid Extracts, Nurgün Küçükboyaci, Semi̇ha Özkan, Nezaket Adigüzel, Fatma Tosun
Turkish Journal of Biology
The aerial parts and seeds of Sophora alopecuroides L. var. alopecuroides growing in Turkey were investigated for their alkaloid compositions and antimicrobial activities. The alkaloid extracts were analysed by capillary gas chromatography-mass spectrometry (GC-MS). The main components were identified as sophoridine (33.07%), matrine (24.03%), and sophocarpine (8.01%) in the aerial parts, and matrine (36.67%) and sophoridine (34.53%) in the seeds of the plant. The alkaloid extracts were also evaluated for their in vitro antibacterial and antifungal activities using microdilution techniques. The alkaloid extract of the aerial parts of S. alopecuroides var. alopecuroides presented significant activity against Staphylococcus aureus and Bacillus …
Testing The Putative Effect Of Dicer-Substrate Sirnas In Regulating Gene Expression At Transcriptional Level, Müge Kovancilar, Mehmet Nejat Dalay, Uğur Deli̇gezer
Testing The Putative Effect Of Dicer-Substrate Sirnas In Regulating Gene Expression At Transcriptional Level, Müge Kovancilar, Mehmet Nejat Dalay, Uğur Deli̇gezer
Turkish Journal of Biology
RNA interference (RNAi) pathway is a gene silencing process during which small double-stranded RNA (dsRNA) molecules trigger the degradation of homologous RNA targets. Small interfering RNAs (siRNAs) are the mediators of the RNAi that can be induced in vitro and in vivo by direct application of chemically synthesized siRNAs. Recently, promoter-targeted small non-coding RNAs have been described to be capable of regulating gene expression at the transcriptional level. In the present study we tested the hypothesis that Dicer-substrate siRNAs (D-siRNAs), which trigger gene silencing through intrinsic RNAi pathway and are therefore more potent in gene knockdown than traditionally used 21-23mer …
Expression Of Β-(1,3-1,4)-Glucanase Gene Of Orpinomyces Sp. Gmlf18 In Escherichia Coli Ec1000 And Lactococcus Lactis Subsp. Cremoris Mg1363, Uğur Çömlekci̇oğlu, Emi̇n Özköse, İsmai̇l Akyol, Feri̇t Can Yazdiç, Mehmet Sai̇t Eki̇nci̇
Expression Of Β-(1,3-1,4)-Glucanase Gene Of Orpinomyces Sp. Gmlf18 In Escherichia Coli Ec1000 And Lactococcus Lactis Subsp. Cremoris Mg1363, Uğur Çömlekci̇oğlu, Emi̇n Özköse, İsmai̇l Akyol, Feri̇t Can Yazdiç, Mehmet Sai̇t Eki̇nci̇
Turkish Journal of Biology
A gene encoding \beta-(1,3-1,4)-glucanase (licA) was amplified from Orpinomyces sp. GMLF18 and expressed in Escherichia coli. The DNA sequence of licA showed that the gene was 707 bp and encoded a protein with a molecular mass of 26 kDa that belongs to family glycosyl hydrolase 16. The main LicA activity was observed to be cell-associated for the licA containing transformant E. coli, and the enzyme expressed in E. coli showed the highest activity at pH 5.0-6.0 and at temperatures of 40-50 °C. The enzyme was found to be stable at 40 °C; however, 12% of LicA activity was lost at …
The Application Of Issr-Pcr To Determine The Genetic Relationship And Genetic Diversity Between Narrow Leaved Bluegrass (Poa Angustifolia) And Rough Bluegrass (Poa Trivialis) Accessions, Emi̇ne Arslan, Ahmet Tamkoç
The Application Of Issr-Pcr To Determine The Genetic Relationship And Genetic Diversity Between Narrow Leaved Bluegrass (Poa Angustifolia) And Rough Bluegrass (Poa Trivialis) Accessions, Emi̇ne Arslan, Ahmet Tamkoç
Turkish Journal of Biology
: The present study aimed to determine the genetic relationship between Poa angustifolia and Poa trivialis as well as intraspecies genetic diversity using inter simple sequence repeat (ISSR) markers. ISSR amplification was evaluated for its applicability to narrow leaved bluegrass and rough bluegrass accessions identification. A total of 401 bands, 363 of which were polymorphic (90.52%), were generated using selected 20 primers. Genetic similarity between accessions belonging to Poa species was estimated using Nei's genetic similarity and UPGMA (unweighted pair group method with arithmetic) cluster analysis was used to construct a dendrogram. UBC836 and M6 primers were observed to be …
Changes In The Hemolymph Total Protein Of Galleria Mellonella (Lepidoptera: Pyralidae) After Parasitism And Envenomation By Pimpla Turionellae (Hymenoptera: Ichneumonidae), Olga Sak, Ekrem Ergi̇n, Fevzi̇ Uçkan, David B. Rivers, Ayli̇n Er
Changes In The Hemolymph Total Protein Of Galleria Mellonella (Lepidoptera: Pyralidae) After Parasitism And Envenomation By Pimpla Turionellae (Hymenoptera: Ichneumonidae), Olga Sak, Ekrem Ergi̇n, Fevzi̇ Uçkan, David B. Rivers, Ayli̇n Er
Turkish Journal of Biology
Venom from the endoparasitoid Pimpla turionellae L. (Hymenoptera: Ichneumonidae) contains a mixture of biologically active components, which display potent paralytic, cytotoxic, and cytolytic effects towards hosts. Here, we further investigate if parasitism or envenomation by P. turionellae alters total protein of its host Galleria mellonella L. (Lepidoptera: Pyralidae). Various venom concentrations representing doses previously determined to yield host responses yet fall below the calculated LD99 were used for pupae and larvae. Parasitization was only assayed for host pupa since P. turionellae females normally parasitize host prepupae and pupae in nature. Hemolymph total protein concentration remained relatively steady at all doses …
Effects Of Cold And Salicylic Acid Treatments On Nitrate Reductase Activity In Spinach Leaves, Beli̇nda Aydin, Barbaros Nalbantoğlu
Effects Of Cold And Salicylic Acid Treatments On Nitrate Reductase Activity In Spinach Leaves, Beli̇nda Aydin, Barbaros Nalbantoğlu
Turkish Journal of Biology
The effects of long-term cold (5/3 °C) and salicylic acid (SA) (0.1 mM) treatments on the diurnal pattern of nitrate reductase (NR) activity in spinach (Spinacia oleracea L. cv. Gladiator) leaves were investigated. When the activities with cold treatment are compared to the control (20/15 °C), NR activity was decreased by 5 °C in the light period and by 3 °C in the dark period. The cold treatment also affected the timing of circadian rhythm in the diurnal pattern of NR activity. When the activities with SA treatments are compared to control and cold, it was determined that SA increases …
Phenolic Constituents Of Hypericum Triquetrifolium Turra (Guttiferae) Growing In Turkey: Variation Among Populations And Plant Parts, Cüneyt Çirak, Jolita Radusiene, Valdimaras Janulis, Liudas Ivanauskas, Necdet Çamaş, Ali̇ Kemal Ayan
Phenolic Constituents Of Hypericum Triquetrifolium Turra (Guttiferae) Growing In Turkey: Variation Among Populations And Plant Parts, Cüneyt Çirak, Jolita Radusiene, Valdimaras Janulis, Liudas Ivanauskas, Necdet Çamaş, Ali̇ Kemal Ayan
Turkish Journal of Biology
Hypericum triquetrifolium Turra (Guttiferae) has received considerable scientific interest in recent years, as it is a source of a variety of biologically active compounds including phenolics. The present study was conducted to determine the variation in the content of several phenolics, namely phenylpropane chlorogenic acid and flavonoids such as rutin, hyperoside, apigenin-7-O-glucoside, kaempferol, quercitrin, quercetin and amentoflavone among four H. triquetrifolium populations from the Central Black Sea Region of Turkey. The aerial parts representing a total of 30 shoots were collected at full flowering in each population. After drying at room temperature, they were dissected into floral, leaf and stem …
In Vitro Antagonistic Activity Of Fungi Isolated From Sclerotia On Potato Tubers Against Rhizoctonia Solani, Erkol Demi̇rci̇, Eli̇f Dane, Cafer Eken
In Vitro Antagonistic Activity Of Fungi Isolated From Sclerotia On Potato Tubers Against Rhizoctonia Solani, Erkol Demi̇rci̇, Eli̇f Dane, Cafer Eken
Turkish Journal of Biology
Forty-five fungal isolates were obtained from sclerotia of Rhizoctonia solani on potato tubers in Erzurum, Turkey. The interaction between fungal isolates and R. solani was studied in dual culture technique. Some fungal isolates affected R. solani by antibiosis and/or parasitism. Results of the antagonism tests showed that Acremonium sp., Gliocladium viride, Paecilomyces marquandii, Paecilomyces sulphurellus, Penicillium camemberti, Penicillium expansum, Penicillium frequentans (ME-50), Penicillium nigricans, Penicillium olsonii, Penicillium phialosporum, Sporothrix sp. (MCY-4), Sporothrix schenckii, and Verticillium dahliae isolates produced an inhibition zone in front of the R. solani colony to a varying degree. Trichoderma harzianum isolates were able to overgrow the …
Seasonal Variations In Phytoplankton Composition And Biomass In A Small Lowland River-Lake System (Melen River, Turkey), Tülay Baykal, İlkay Açikgöz, Abel U. Udoh, Kazim Yildiz
Seasonal Variations In Phytoplankton Composition And Biomass In A Small Lowland River-Lake System (Melen River, Turkey), Tülay Baykal, İlkay Açikgöz, Abel U. Udoh, Kazim Yildiz
Turkish Journal of Biology
A series of studies were carried out to determine the succession and phytoplankton community of the Melen River in the Western Black Sea region of Turkey. The study was conducted at 6 stations at monthly intervals between May 2003 and April 2004. It was observed that the abundance and biovolume of phytoplankton were low in winter, though higher values were recorded in late spring and summer. Among the values noted, the spring peak was 534.38 × 10^5 ind. L^{-1} and 7384.8 mm^3 L^{-1} belonging to Peridinium sp. The most common diatom, Cyclostephanos dubius, was favoured by low discharge at stations …
Antibacterial Activity Of Bee Propolis Samples From Different Geographical Regions Of Turkey Against Two Foodborne Pathogens, Salmonella Enteritidis And Listeria Monocytogenes, Ayhan Temi̇z, Ayla Şener, Asli Özkök Tüylü, Kadri̇ye Sorkun, Beki̇r Sali̇h
Antibacterial Activity Of Bee Propolis Samples From Different Geographical Regions Of Turkey Against Two Foodborne Pathogens, Salmonella Enteritidis And Listeria Monocytogenes, Ayhan Temi̇z, Ayla Şener, Asli Özkök Tüylü, Kadri̇ye Sorkun, Beki̇r Sali̇h
Turkish Journal of Biology
The aim of the present study was to investigate the antibacterial activities of 25 propolis samples collected from various geographical regions of Turkey against 2 food-borne pathogens, Salmonella Enteritidis ATCC 13076 and Listeria monocytogenes ATCC 1462. The chemical compositions of ethyl alcohol extracts of the propolis (EEP) samples were determined by gas chromatography coupled to mass spectrometry. The main components of EEP samples were flavonoids, aromatic acid esters, aromatic alcohols, aromatic acids, aliphatic carboxylic acids, terpens, and aliphatic carboxylic acid esters. Antibacterial activities of the EEP samples were tested at 2 different dilutions of 1:10 and 1:100 (v/v). All EEP …
Influence Of Different Sterilization Methods On Callus Initiation And Production Of Pigmented Callus In Arnebia Densiflora Ledeb., Hati̇ce Çölgeçen, Ufuk Koca Çalişkan, Gülnur Toker
Influence Of Different Sterilization Methods On Callus Initiation And Production Of Pigmented Callus In Arnebia Densiflora Ledeb., Hati̇ce Çölgeçen, Ufuk Koca Çalişkan, Gülnur Toker
Turkish Journal of Biology
We analyzed the effects of sodium hypochlorite and Plant Preservative Mixture (PPM) on surface sterilization. We also examined the effects that the addition of an antibiotic-antimycotic solution to the culture medium had on callus induction. Explants were initially sterilized with different concentrations of sodium hypochlorite and cultured on MS media containing kinetin (0.29 \muM) and naphthalene acetic acid (NAA, 10 \muM). No calluses were produced, either because of contamination of the explants, or loss of explants as a result of the high levels of sodium hypochlorite. The application of PPM and antibiotics at different concentrations reduced contamination and led to …
The Interaction Between Renin-Angiotensin System And Sympathetic Nervous System In The Peripheral Vasculature Of Normal Sprague-Dawley Rats, Mohammed H. Abdulla, Munavvar A. Sattar, Nor A. Abdullah, Kolla Rl. Anand Swarup, Abdul Hye Khan, Edward J. Johns
The Interaction Between Renin-Angiotensin System And Sympathetic Nervous System In The Peripheral Vasculature Of Normal Sprague-Dawley Rats, Mohammed H. Abdulla, Munavvar A. Sattar, Nor A. Abdullah, Kolla Rl. Anand Swarup, Abdul Hye Khan, Edward J. Johns
Turkish Journal of Biology
To evaluate possible interactions between the renin-angiotensin system (RAS) and the sympathetic nervous system (SNS) in the systemic circulation upon the intravenous administration of angiotensin II and a set of adrenergic agonists in normal Sprague-Dawley (SD) rats. Rats were treated with carvedilol, a non-selective \beta- and selective \alpha_1-adrenoceptor blocker, in a dose of (5 mg/kg) orally for 7 days, and on day 8, the animals were pentobarbitone-anaesthetized and prepared for systemic hemodynamic study. Dose-response relationships in terms of elevation in the magnitude of the mean arterial blood pressure in response to intravenous injection of noradrenaline (NA), phenylephrine (PE), methoxamine (ME), …
Isolation And Characterization Of Xylanolytic New Strains Of Anoxybacillus From Some Hot Springs In Turkey, Kadri̇ye İnan, Sabri̇ye Çanakçi, Ali̇ Osman Beldüz
Isolation And Characterization Of Xylanolytic New Strains Of Anoxybacillus From Some Hot Springs In Turkey, Kadri̇ye İnan, Sabri̇ye Çanakçi, Ali̇ Osman Beldüz
Turkish Journal of Biology
Some hot springs located in the west of Turkey were investigated regarding the presence of xylanolytic thermophilic microorganisms. Based on phenotyping characteristics and 16S rRNA gene sequence analysis, 9 of these xylanolytic isolates belonged to the genus Anoxybacillus, and grew optimally at about 50-60 °C on nutrient agar. The 16S rRNA gene sequence analysis showed that these isolates resembled the Anoxybacillus species =>97 and these isolates are members of the genus Anoxybacillus. Based on the DNA-DNA hybridization study, SDS-PAGE profile, and biochemical and physiological features, I3, CT1Sari, and BT2.1 isolates are new strains of Anoxybacillus gonensis; I4.2 and B9.3 …
Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu
Isolation, Production, And Characterization Of An Extracellular Lipase From Trichoderma Harzianum Isolated From Soil, Serdar Ülker, Arzu Özel, Ahmet Çolak, Şengül Alpay Karaoğlu
Turkish Journal of Biology
This is the first report about the characterization of Trichoderma harzianum lipase. A novel strain of Trichoderma harzianum IDM14D was isolated from soil. The isolated strain was cultivated for lipase production in shake flasks at 30 °C for 7 days. For lipase production, it was determined that the best carbon source was glucose and the best nitrogen source was peptone. Maximum biomass was produced at a concentration of 1.25 g/L, in 7 days. The optimum pH and temperature for activity of the enzyme were 8.5 and 40 °C, respectively. The lipase was stable at a pH range of 8.0-10.0 and …
Assessment Of Potential Plastic-Degrading Fungi In Jordanian Habitats, Ibrahim N. Ibrahim, Anwar Maraqa, Khalid M. Hameed, Ismail M. Saadoun, Hamzah M. Maswadeh
Assessment Of Potential Plastic-Degrading Fungi In Jordanian Habitats, Ibrahim N. Ibrahim, Anwar Maraqa, Khalid M. Hameed, Ismail M. Saadoun, Hamzah M. Maswadeh
Turkish Journal of Biology
Out of 70 fungal isolates recovered from soil, wall paints (Latex), and pieces of plastic debris from different habitats in Jordan and plastic shields of street light posts on campus, 35 isolates showed varied potential to degrade polyester-polyurethane (PS-PUR). Six of these isolates (Fusarium solani, Alternaria solani, Spicaria spp., Aspergillus fumigatus, Aspergillus terreus, and Aspergillus flavus) were selected on the basis of their growth rates on basal salt media amended with PS-PUR as the sole source of carbon. Isolates were further evaluated utilizing 3 different methods: 1) direct plating, 2) clear zone in a 2-layered agar media, and 3) liquid …
Acid Proteinase Enzyme Activity In Candida Albicans Strains: A Comparison Of Spectrophotometry And Plate Methods, Sevi̇m Akçağlar, Beyza Ener, Okan Töre
Acid Proteinase Enzyme Activity In Candida Albicans Strains: A Comparison Of Spectrophotometry And Plate Methods, Sevi̇m Akçağlar, Beyza Ener, Okan Töre
Turkish Journal of Biology
In recent years, the incidence of fungal infections has been rising all over the world. Although the amount of research in the field of pathogenic fungi has also increased, there is still a need for studies on fungal virulence. In this investigation, we focused on testing bloodstream-identified Candida albicans in experimental in vitro virulence assays. The proteinase enzyme activities of 30 C. albicans strains isolated from the bloodstream and sterile body fluids were investigated (15 colonizations, 15 infections). The spectrophotometric and plate methods were used to determine proteinase activity. The correlations between the 2 methods were compared. This study indicates …
A Comparative Study On Alkaline Lipase Production By A Newly Isolated Aspergillus Fumigatus Mtcc 9657 In Submerged And Solid-State Fermentation Using Economically And Industrially Feasible Substrate, Akhila Rajan, A. Jayakumaran Nair
A Comparative Study On Alkaline Lipase Production By A Newly Isolated Aspergillus Fumigatus Mtcc 9657 In Submerged And Solid-State Fermentation Using Economically And Industrially Feasible Substrate, Akhila Rajan, A. Jayakumaran Nair
Turkish Journal of Biology
A comparative study of solid-state fermentation (SSF) and submerged fermentation (SmF) for the production of alkaline lipase from a new isolate, Aspergillus fumigatus MTCC 9657, using defatted rice bran (DFRB) as a substrate was studied. Different process parameters, such as incubation period, initial pH and incubation temperature, were optimised to achieve the maximum yield of the alkaline lipase. Maximum enzyme production (8.13 U/mL) was obtained by day 7 of incubation in SSF compared to day 4 in SmF at an optimum pH of 8.5 and ambient temperature of 30 °C. Lipase produced by SSF was stable over a period of …
Characterization Of Poly Phenol Oxidase In Two In Vitro Regenerated Cultivars Of Mucuna: Mucuna Pruriens L. And Mucuna Prurita H., Raghavendra Sathyanarayana, Vadlapudi Kumar, Chapeyil Kumaran Ramesh, Mahadevappa Parmesha, Mahaboob Habeebulla Moinuddin Khan
Characterization Of Poly Phenol Oxidase In Two In Vitro Regenerated Cultivars Of Mucuna: Mucuna Pruriens L. And Mucuna Prurita H., Raghavendra Sathyanarayana, Vadlapudi Kumar, Chapeyil Kumaran Ramesh, Mahadevappa Parmesha, Mahaboob Habeebulla Moinuddin Khan
Turkish Journal of Biology
Polyphenol oxidases (PPOs EC 1.14.18.1) were isolated from Mucuna pruriens and Mucuna prurita and confirmed as tyrosinases involved in L-DOPA production. PPOs were extracted by using a 0.05 M phosphate buffer, pH 7.0. The purified enzyme was resolved into a single band by PAGE, the enzyme was confirmed as PPO by activity staining, and SDS-PAGE analysis revealed that the purified PPO of both the species is a tetramer. Substrate specificity experiments were carried out with catechol, L-DOPA, L-tyrosine, and p-cresol. Of these, catechol was evaluated as the most suitable substrate based on the determined K_{m} and V_{max} values. The optimum …
Increased Production Of Coleoptera-Specific Delta-Endotoxin By Homologous Expression Of Cry3aa11 In Bacillus Thuringiensis Mm2, Aslihan Kurt, Gülay Özcengi̇z
Increased Production Of Coleoptera-Specific Delta-Endotoxin By Homologous Expression Of Cry3aa11 In Bacillus Thuringiensis Mm2, Aslihan Kurt, Gülay Özcengi̇z
Turkish Journal of Biology
The cry3Aa11 gene of Bacillus thuringiensis strain Mm2 (B. thuringiensis Mm2) was PCR-amplified and cloned into E. coli/B. thuringiensis shuttle vector pHT315. The recombinant plasmid carrying the cry3Aa11 gene was introduced into the parental B. thuringiensis Mm2. The toxin production capacities of parental and recombinant strains grown in DSM, containing either 50 mM or 200 mM inorganic phosphate (Pi), were compared by SDS-PAGE and western blot analyses. In comparison to the parental strain, recombinant B. thuringiensis Mm2 produced 4-fold more Cry3Aa11 toxin after 12 h of growth in the presence of 50 mM Pi. However, the 200 mM Pi concentration …
Phosphate Solubility And Biocontrol Activity Of Trichoderma Harzianum, Padmavathi Tallapragada, Madhumathi Gudimi
Phosphate Solubility And Biocontrol Activity Of Trichoderma Harzianum, Padmavathi Tallapragada, Madhumathi Gudimi
Turkish Journal of Biology
Biological control of soilborne plant pathogens is a possible alternative to the use of chemical pesticides, which are harmful to the environment. Under greenhouse and field conditions, several isolated strains of the fungus Trichoderma have been found to be effective biocontrol agents of various soilborne plant pathogenic fungi. The use of phosphate solubilizing microorganisms (PSMs) has a considerable synergistic effect on the growth and development of crop plants. In the present study, T. harzianum was isolated from 10 different agro-climatic zones of Karnataka and tested for the potential of antagonistic activity against the plant pathogen Xanthomonas and the ability of …