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Section Abstracts: Biology With Microbiology And Molecular Biology

Virginia Journal of Science

Abstracts of the Biology with Microbiology and Molecular Biology Section for the 89th Annual Meeting of the Virginia Academy of Science, May 25-27, 2011, University of Richmond, Richmond VA.

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Section Abstracts: Natural History And Biodiversity

Virginia Journal of Science

Abstracts of the Natural History and Biodiversity Section for the 89th Annual Meeting of the Virginia Academy of Science, May 25-27, 2011, University of Richmond, Richmond VA.

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Uptake Of Nanoparticles Of Cerium Oxide And Yttrium Oxide By Acanthamoeba Castellanii (Protozoa) And Daphnia Magna (Crustacea), James R. Palmieri, Geneva Gehring, Catherine Minichino, Shaadi F. Elswaifi

Virginia Journal of Science

Currently, nanoparticles are synthesized and used at an unprecedented rate for industrial, medical, and research applications. The use of cerium oxide nanoparticles (CeONP) and yttrium oxide nanoparticles (YtONP) results in their spread as contaminants into the environment. Once in the environment, CeONP and YtONP can be taken up by organisms in the food chain where they may pose a public health risk. In this study we determine whether Acanthamoeba castellanii and Daphnia magna uptake CeONP or YtONP from their environment and thereby play a role in the transmission of the nanoparticles. Using electron microscopy, orgranisms exposed to the nanoparticles were …

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Preliminary Analysis Of An Agent-Based Model For A Tick-Borne Disease, Holly Gaff

Biological Sciences Faculty Publications

Ticks have a unique life history including a distinct set of life stages and a single blood meal per life stage. This makes tick-host interactions more complex from a mathematical perspective. In addition, any model of these interactions must involve a significant degree of stochasticity on the individual tick level. In an attempt to quantify these relationships, I have developed an individual-based model of the interactions between ticks and their hosts as well as the transmission of tick-borne disease between the two populations. The results from this model are compared with those from previously published differential equation based population models. …

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Advertisement Call And Distribution Of The Treefrogs Hyla Chrysoscelis And Hyla Versicolor In Virginia, Joseph C. Mitchell, Christopher A. Pague

Virginia Journal of Science

The gray treefrog complex consists of two sibling species that are indistinguishable morphologically, the diploid Hyla chrysoscelis and the tetraploid Hyla versicolor. Identification is possible in the field only by audio recognition of male advertisement call trill rates (pulses/second). During 1979-1983 we evaluated taped calls of these two species taken from 89 populations from throughout Virginia to map their respective ranges and to evaluate differences in call parameters. Hyla chrysoscelis occurs in the Coastal Plain, eastern and southern Piedmont, and in southwestern Virginia. Hyla versicoloroccurs in the Piedmont, Blue Ridge, and Ridge and Valley regions south to Wythe …

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Analysis Of Biological Features Associated With Meiotic Recombination Hot And Cold Spots In Saccharomyces Cerevisiae, Loren Hansen, Nak-Kyeong Kim, Leonardo Mariño-Ramírez, David Landsman

Mathematics & Statistics Faculty Publications

Meiotic recombination is not distributed uniformly throughout the genome. There are regions of high and low recombination rates called hot and cold spots, respectively. The recombination rate parallels the frequency of DNA double-strand breaks (DSBs) that initiate meiotic recombination. The aim is to identify biological features associated with DSB frequency. We constructed vectors representing various chromatin and sequence-based features for 1179 DSB hot spots and 1028 DSB cold spots. Using a feature selection approach, we have identified five features that distinguish hot from cold spots in Saccharomyces cerevisiae with high accuracy, namely the histone marks H3K4me3, H3K14ac, H3K36me3, and H3K79me3; …

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Distributions And Abundances Of Microstegium Vimineum Along Forest Roadsides At The Grassy Hill Natural Area, Franklin County, Virginia, Gregory D. Turner

Virginia Journal of Science

In summer 2005, plots were surveyed along roads passing through forest habitats at the Grassy Hill Natural Area Preserve in Franklin County, Virginia to assess the distributions and abundances of Microstegium vimineum in transects located at increasing distances away from roadsides into forest interiors. Across plots, Microstegium was encountered almost exclusively in roadside transects, where abundances were relatively high. While forest composition and topographic features were similar across plots, percent canopy cover and leaf litter depth were greater in interior compared to roadside transects due to undisturbed tree canopies and ground cover located in interior plot areas. Results imply that …

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A New Species Of The Subterranean Amphipod Crustacean Genus Stygobromus (Crangonyctidae) From A Cave In Nevada, Usa, Steven J. Taylor, John R. Holsinger

Biological Sciences Faculty Publications

Stygobromus albapinus, a new stygobiotic amphipod crustacean species in the family Crangonyctidae, is described from two pools in Model Cave in Great Basin National Park, White Pine County, Nevada, USA. The type specimens were collected on two different visits to the cave. The new species is assigned to the hubbsi group, bringing the number of described species in this group to 45, but many other provisionally recognized species assigned to this group remain undescribed. With exception of a single species from deep wells in southeastern Wisconsin, all other members of the hubbsi group are recorded from a wide variety …

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Putting The X In Biology: A Review Of The Mathematics Of Life, John Adam

Mathematics & Statistics Faculty Publications

(First Paragraph) Charles Darwin's 1859 work On the Origin of the Species contained no equations. But that does not mean mathematics has no role to play in the science of life; in fact, the field of biomathematics is burgeoning and has been for several decades. Ian Stewart's new book does an admirable job of unfolding the mathematics undergirding so much of the research being carried out today in the many fields that comprise the subject of biology. Stewart sets the context by noting five great revolutions that have changed the way scientists think about life. These five revolutions are: (i) …

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Gulf Of Mexico Oil Blowout Increases Risks To Globally Threatened Species, Claudio Campagna, Frederick T. Short, Beth A. Polidoro, Roger Mcmanus, Bruce B. Collette, Nicolas J. Pilcher, Yvonne Sadovy De Mitcheson, Simon N. Stuart, Kent E. Carpenter

Biological Sciences Faculty Publications

Fourteen marine species in the Gulf of Mexico are protected by the US Endangered Species Act, the Marine Mammal Protection Act, and the Migratory Bird Treaty Act. As the British Petroleum oil spill recovery and remediation proceed, species internationally recognized as having an elevated risk of extinction should also receive priority for protection and restoration efforts, whether or not they have specific legal protection. Forty additional marine species-unprotected by any federal laws-occur in the Gulf and are listed as threatened on the International Union for Conservation of Nature's (IUCN) Red List. The Red List assessment process scientifically evaluates species' global …

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Range Extension For The Dismal Swamp Southern Bog Lemming, Synaptomys Cooperi Helaletes, In Eastern Virginia, Robert K. Rose

Biological Sciences Faculty Publications

The distribution of the Dismal Swamp Southern Bog Lemming is extended to include three counties west of the Dismal Swamp in southeastern Virginia, long considered its only location in the state. Evidence of lemmings was detected at 10 of 27 survey sites, and confirmed by trapping at three of five sites, all dominated by herbaceous vegetation.

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Classroom Manipulative To Engage Students In Mathematical Modeling Of Disease Spread: 1 + 1 = Achoo!, H. Gaff, M. Lyons, G. Watson

Biological Sciences Faculty Publications

Infectious diseases ranging from the common cold to cholera affect our society physically, emotionally, ecologically, and economically. Yet despite their importance and impact, there remains a lack of effective teaching materials for epidemiology and disease ecology in K-12, undergraduate, and graduate curricula [2]. To address this deficit, we've developed a classroom lesson with three instructional goals: (1) Familiarize students on basic concepts of infectious disease ecology; (2) Introduce students to a classic compartmental model and its applications in epidemiology; (3) Demonstrate the application and importance of mathematical modeling as a tool in biology. The instructional strategy uses a game-based mathematical …

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Behavior Constrains The Dispersal Of Long-Lived Spiny Lobster Larvae, Mark J. Butler Iv, Claire B. Paris, Jason S. Goldstein, Hirokazu Matsuda, Robert K. Cowen

Biological Sciences Faculty Publications

Behavior such as ontogenetic vertical migration (OVM) limits the transport of marine larvae with short pelagic larval durations (PLDs), but its effect on the supposed long-distance dispersal of larvae with long PLDs is unknown. We conducted laboratory tests of ontogenetic change in larval phototaxis and examined size-specific patterns of larval distribution in the plankton to characterize OVM in the Caribbean spiny lobster Panulirus argus during its long (6 mo) PLD. We then used a coupled biophysical model to explore the consequences of OVM and hydrodynamics on larval P. argus dispersal in the Caribbean Sea. Larvae reared in the laboratory were …

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Foundational Checklist Of The Amphibians Of Wise County, Virginia, Sarah R.A. Davidson, David L. Chambers

Virginia Journal of Science

The Appalachian Mountains are arguably home to the highest degree of amphibian diversity in the world, particularly caudate (salamander) biodiversity. Despite the high degree of amphibian endemism in the Appalachians, several regions remain unsurveyed for amphibian species. In addition to this knowledge gap, we are in the midst of alarming amphibian biodiversity loss. Thus, it is of the utmost importance to bridge this knowledge gap by conducting surveys before some of these amphibian species are lost. We surveyed Wise County (previously unsurveyed county in the Appalachian Mountains with no records existing in the primary literature) over two years to assess …

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"Beyond Bio2010: Celebration And Opportunities" At The Intersection Of Mathematics And Biology, John R. Jungck, Holly D. Gaff, Adam P. Fagen, Jay B. Labov

Biological Sciences Faculty Publications

With this special edition of CBE-LSE, which focuses on connections between and integration of the biological and mathematical sciences, it is especially fitting that we report on an important symposium, Beyond BIO2010: Celebration and Opportunities,1 which was held at the National Acad- emy of Sciences (NAS) in Washington, D.C. on May 21–22, 2010. This symposium was organized to assess what progress has been made in addressing the challenges and recommendations in the National Research Council’s (NRC) report: BIO2010: Transforming Undergraduate Education for Future Research Biologists (NRC, 2003a). Most of the presen- tations and posters at this event emphasized the increasing …

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Mathematical Manipulative Models: In Defense Of "Beanbag Biology", John R. Jungck, Holly Gaff, Anton E. Weisstein

Biological Sciences Faculty Publications

Mathematical manipulative models have had a long history of influence in biological research and in secondary school education, but they are frequently neglected in undergraduate biology education. By linking mathematical manipulative models in a four-step process-1) use of physical manipulatives, 2) interactive exploration of computer simulations, 3) derivation of mathematical relationships from core principles, and 4) analysis of real data sets-we demonstrate a process that we have shared in biological faculty development workshops led by staff from the BioQUEST Curriculum Consortium over the past 24 yr. We built this approach based upon a broad survey of literature in mathematical educational …

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Section Abstracts: Biology With Microbiology And Molecular Biology

Virginia Journal of Science

Abstracts of the Biology with Microbiology and Molecular Biology Section for the 88th Annual Meeting for the Virginia Academy of Science, May 20-21, 2010, James Madison University, Harrisonburg, VA.

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Corrections To The Paper "Phytoplankton Productivity In The Tidal Regions Of Four Chesapeake Bay (Usa) Tributaries." Virginia Journal Of Science. 2007 58(4): 191-204, By K. K. Nesius, H. G. Marshall, And T.A. Egerton

Virginia Journal of Science

Necessary corrections associated with the paper "Phytoplankton productivity in the tidal regions of four Chesapeake Bay (USA) tributaries", originally published in Virginia Journal of Science Volume 58, Number 4, pages 191-204, in 2007, by K.K. Nesius, H.G. Marshall, and T.A. Egerton.

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Section Abstracts: Natural History And Biodiversity

Virginia Journal of Science

Abstracts of the Natural History and Biodiversity Section for the 88th Annual Meeting of the Virginia Academy of Science, May 20-21, 2010, James Madison University, Harrisonburg, VA.

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Inter-Island Variability In Above And Belowground Plant Biomass In Interior Marshes On The Virginia Barrier Islands, Staci B. Blecha

Biological Sciences Theses & Dissertations

The Virginia Coast Reserve's Long Term Ecological Research (LTER) site is of great interest because the barrier islands have been migrating, rapidly at times, and are exceptionally dynamic. The barrier islands have tidal saltwater marshes on the lagoon side and freshwater marshes on the island interiors. In these interior marshes on North Hog Island, South Smith Island, and North Parramore Island, salinity and depth to water table were measured to determine if these factors were influencing biomass and biomass allocation patterns in the marsh. Hog Island had the freshest interior marshes (0 and 0.7 ppm), and the most diverse (H'=0.84 …

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A Habitat Model For The Detection Of Two-Lined Salamanders At C. F. Phelps Wildlife Management Area, Fauquier And Culpeper Counties, Virginia, Jay Mcghee, Michael D. Killian

Virginia Journal of Science

Aquatic salamanders represent an important component of Virginia river watersheds, but despite potential declines, few specifics are known about their habitat preferences. We surveyed the habitats of the northern two-lined salamander and collected data on an array of habitat variables associated with the species. We used a logistic regression analysis to develop a model predicting its presence or absence for a given 50m-transect. Our final model incorporated the variation in stream depth and direction of stream flow and accounted for 25% of the variation in our data. We conclude that stream depth variation is an important feature of salamander habitat …

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Functional Feeding Groups, Species Richness, And Spatial Distributions Of Fishes In Rocky And Sandy Beach Habitats Of St. John, U.S. Virgin Islands, Eugene G. Maurakis, George E. Maurakis, Demetri E. Maurakis

Virginia Journal of Science

Objectives were to identify and compare fish species richness, functional feeding group richness and diversity, and delineate distributions of fishes at rocky and sandy beach habitats at St. John, U.S. Virgin Islands. Visual observations using snorkel and mask were made at 3-m intervals seaward from shore during daylight hours. A total of 69 taxa (67 species) representing 33 families of fishes were observed. Total (53) and average fish species richness (32.7) at rocky beach habitats were greater than those (total=43; average=24.3) at sandy beach habitats. Twelve functional feeding groups were identified (diurnal planktivores, excavators/eroders, macroalgae browsers, macrocarnivores, mobile benthic scrapers, …

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Conservation Status And Habitat Use Of The West Virginia Spring Salamander (Gyrinophilus Subterraneus) And Spring Salamander (G. Porphyriticus) In General Davis Cave, Greenbrier Co., West Virginia, Matthew L. Niemiller, Michael S. Osbourn, Dante B. Fenolio, Thomas K. Pauley, Brian T. Miller, John R. Holsinger

Biological Sciences Faculty Publications

The West Virginia Spring Salamander (Gyrinophilus subterraneus) is one of four obligate cave-dwelling species of plethodontid salamanders found east of the Mississippi River in the United States. This species is endemic to a single cave system; General Davis Cave, in Greenbrier Co., West Virginia, where it is syntopic with the closely-related Spring Salamander (G. porphyriticus). Accordingly, the West Virginia Spring Salamander is a species of critical conservation concern. Because of it's conservation status and lack of data regarding the ecology and life history, particularly about population trends, we present data on relative abundance of and habitat …

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Impacts Of The Spotted Spiny Lobster (Panulirus Guttatus) On The Long-Spined Sea Urchin (Diadema Antillarum) And Patch Reef Communities In The Florida Keys, Meredith D. Kintzing

Biological Sciences Theses & Dissertations

Caribbean coral reefs have undergone a phase shift from a system dominated by corals to one where algae are pervasive. This shift was precipitated by the loss of herbivores, including the mass mortality of the long spined sea urchin (Diadema antillarum), coupled with disease and the recruitment failure of hermatypic corals. Diadema populations have recovered in some areas of the Caribbean, but are still below historical levels in the Florida Keys, likely due to low larval supply coupled with predation on juveniles. Lobsters are sea urchin predators in other systems and the spotted spiny lobster (Panulirus guttatus …

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First Records Of Hypleurochilus Geminatus And Centropristis Philadelphica From Chesapeake Bay, Aimee D. Halvorson

Virginia Journal of Science

During the fall of 2007, Centropris philadelphica (rock seabass) and Hypleurochilus geminatus (Crested blenny) were collected from Chesapeake Bay. These captures are significant as they represent the first substantiated record of C. philadelphica from Chesapeake Bay and only the second and third validated records of H. geminatus. Additionally, the first record of H. geminatus from Chesapeake Bay was only recently recognized since the specimen had been previously misidentified as Parablennius marmoreus (seaweed blenny). The collection of seven individuals of H. geminatus in 2007, from two locations, indicates that the species may be resident within the Chesapeake Bay estuary.

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Phytoplankton Blooms: Their Occurrence And Composition Within Virginia's Tidal Tributaries, Harold G. Marshall, Todd A. Egerton

Virginia Journal of Science

Sporadic algal bloom development within a 10 year monitoring program in Virginia tidal tributaries of Chesapeake Bay is reviewed. These blooms were common events, characteristically producing a color signature to the surface water, typically short lived, occurring mainly from spring into autumn throughout different salinity regions of these rivers, and were produced primarily by dinoflagellates. The abundance threshold levels that would identify bloom status from a non-bloom presence were species specific, varied with the taxon's cell size, and ranged from ca. 10 to 10⁴cells mL^-1. Among the most consistent sporadic bloom producers were the dinoflagellates Akashiwo …

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Section Abstracts: Biology And Microbiology & Molecular Biology

Virginia Journal of Science

Abstracts of the Biology Section and Microbiology & Molecular Biology for the 87th Annual Meeting of the Virginia Academy of Science, May 27th-29th, 2009, Virginia Commonwealth University, Richmond, VA.

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Biotic And Abiotic Characteristics Influencing Nest Location And Trophic Relationships Of The Fungus-Growing Ant Trachymyrmex Septentrionalis (Formicidae: Attini), Jonathan Paul Howell

Biological Sciences Theses & Dissertations

Trachymyrmex septentrionalis represents the only species of fungus-growing ant in the southeastern United States. T. septentrionalis cultivates a symbiotic fungus on which the ants feed. Worker ants collect plant and animal debris to feed the fungus. These ants are common in Florida, but increasingly scarce as they near the northern extent of their range in New Jersey. Colonies of T. septentrionalis occur in patches throughout Blackwater Ecological Preserve, a longleaf pine forest in southeastern Virginia. Blackwater Ecologic Preserve is a 129 hectare tract of land, located in Isle of Wight county, that is undergoing a regimen of controlled burning to …

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Seasonal Variation In Diet Of A Marginal Population Of The Hispid Cotton Rat, Sigmodon Hispidus, Lynn A. Walker, Robert K. Rose

Virginia Journal of Science

Cotton rats live in oldfields, habitats with a variety of mostly herbaceous plants. Based on other studies, the hispid cotton rat, Sigmodon hispidus, eats many kinds of herbaceous plants but grasses predominate. In contrast, our population of cotton rats ate many monocots but mostly they were not grasses. Our study sought to determine the diet of the cotton rat in eastern Virginia, near the northern limit of distribution on the Atlantic Coast. Fecal samples, collected each month during an on-going capture-mark-release demographic study of the rodent community, were analyzed using a standard method. A greater variety of foods (including …

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Graminicolous Fungi Of Virginia: Fungi In Collections 2004-2007, Curtis W. Roane

Virginia Journal of Science

Fungus-grass associations recognized in Virginia from 2004 to 2007 are recorded. Many associations are new to the United States (U), eastern United States (EU) and Virginia (V); other associations extend the known distribution of those previously discovered. These reports contribute further to knowledge of the mycoflora of Virginia.

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Full-Text Articles in Biology

Section Abstracts: Biology With Microbiology And Molecular Biology

Virginia Journal of Science

Section Abstracts: Natural History And Biodiversity

Virginia Journal of Science

Uptake Of Nanoparticles Of Cerium Oxide And Yttrium Oxide By Acanthamoeba Castellanii (Protozoa) And Daphnia Magna (Crustacea), James R. Palmieri, Geneva Gehring, Catherine Minichino, Shaadi F. Elswaifi

Virginia Journal of Science

Preliminary Analysis Of An Agent-Based Model For A Tick-Borne Disease, Holly Gaff

Biological Sciences Faculty Publications

Advertisement Call And Distribution Of The Treefrogs Hyla Chrysoscelis And Hyla Versicolor In Virginia, Joseph C. Mitchell, Christopher A. Pague

Virginia Journal of Science

Analysis Of Biological Features Associated With Meiotic Recombination Hot And Cold Spots In Saccharomyces Cerevisiae, Loren Hansen, Nak-Kyeong Kim, Leonardo Mariño-Ramírez, David Landsman

Mathematics & Statistics Faculty Publications

Distributions And Abundances Of Microstegium Vimineum Along Forest Roadsides At The Grassy Hill Natural Area, Franklin County, Virginia, Gregory D. Turner

Virginia Journal of Science

A New Species Of The Subterranean Amphipod Crustacean Genus Stygobromus (Crangonyctidae) From A Cave In Nevada, Usa, Steven J. Taylor, John R. Holsinger

Biological Sciences Faculty Publications

Putting The X In Biology: A Review Of The Mathematics Of Life, John Adam

Mathematics & Statistics Faculty Publications

Gulf Of Mexico Oil Blowout Increases Risks To Globally Threatened Species, Claudio Campagna, Frederick T. Short, Beth A. Polidoro, Roger Mcmanus, Bruce B. Collette, Nicolas J. Pilcher, Yvonne Sadovy De Mitcheson, Simon N. Stuart, Kent E. Carpenter

Biological Sciences Faculty Publications

Range Extension For The Dismal Swamp Southern Bog Lemming, Synaptomys Cooperi Helaletes, In Eastern Virginia, Robert K. Rose

Biological Sciences Faculty Publications

Classroom Manipulative To Engage Students In Mathematical Modeling Of Disease Spread: 1 + 1 = Achoo!, H. Gaff, M. Lyons, G. Watson

Biological Sciences Faculty Publications

Behavior Constrains The Dispersal Of Long-Lived Spiny Lobster Larvae, Mark J. Butler Iv, Claire B. Paris, Jason S. Goldstein, Hirokazu Matsuda, Robert K. Cowen

Biological Sciences Faculty Publications

Foundational Checklist Of The Amphibians Of Wise County, Virginia, Sarah R.A. Davidson, David L. Chambers

Virginia Journal of Science

"Beyond Bio2010: Celebration And Opportunities" At The Intersection Of Mathematics And Biology, John R. Jungck, Holly D. Gaff, Adam P. Fagen, Jay B. Labov

Biological Sciences Faculty Publications

Mathematical Manipulative Models: In Defense Of "Beanbag Biology", John R. Jungck, Holly Gaff, Anton E. Weisstein

Biological Sciences Faculty Publications

Section Abstracts: Biology With Microbiology And Molecular Biology

Virginia Journal of Science

Corrections To The Paper "Phytoplankton Productivity In The Tidal Regions Of Four Chesapeake Bay (Usa) Tributaries." Virginia Journal Of Science. 2007 58(4): 191-204, By K. K. Nesius, H. G. Marshall, And T.A. Egerton

Virginia Journal of Science

Section Abstracts: Natural History And Biodiversity

Virginia Journal of Science

Inter-Island Variability In Above And Belowground Plant Biomass In Interior Marshes On The Virginia Barrier Islands, Staci B. Blecha

Biological Sciences Theses & Dissertations

A Habitat Model For The Detection Of Two-Lined Salamanders At C. F. Phelps Wildlife Management Area, Fauquier And Culpeper Counties, Virginia, Jay Mcghee, Michael D. Killian

Virginia Journal of Science

Functional Feeding Groups, Species Richness, And Spatial Distributions Of Fishes In Rocky And Sandy Beach Habitats Of St. John, U.S. Virgin Islands, Eugene G. Maurakis, George E. Maurakis, Demetri E. Maurakis

Virginia Journal of Science

Biological Sciences Faculty Publications

Impacts Of The Spotted Spiny Lobster (Panulirus Guttatus) On The Long-Spined Sea Urchin (Diadema Antillarum) And Patch Reef Communities In The Florida Keys, Meredith D. Kintzing

Biological Sciences Theses & Dissertations

First Records Of Hypleurochilus Geminatus And Centropristis Philadelphica From Chesapeake Bay, Aimee D. Halvorson

Virginia Journal of Science

Phytoplankton Blooms: Their Occurrence And Composition Within Virginia's Tidal Tributaries, Harold G. Marshall, Todd A. Egerton

Virginia Journal of Science

Section Abstracts: Biology And Microbiology & Molecular Biology

Virginia Journal of Science

Biotic And Abiotic Characteristics Influencing Nest Location And Trophic Relationships Of The Fungus-Growing Ant Trachymyrmex Septentrionalis (Formicidae: Attini), Jonathan Paul Howell

Biological Sciences Theses & Dissertations

Seasonal Variation In Diet Of A Marginal Population Of The Hispid Cotton Rat, Sigmodon Hispidus, Lynn A. Walker, Robert K. Rose

Virginia Journal of Science

Graminicolous Fungi Of Virginia: Fungi In Collections 2004-2007, Curtis W. Roane

Virginia Journal of Science