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Full-Text Articles in Biology

Prospects Of Kenaf As An Alternative Field Crop In Virginia, Harbans L. Bhardwaj, Charles L. Webber Iii Oct 2005

Prospects Of Kenaf As An Alternative Field Crop In Virginia, Harbans L. Bhardwaj, Charles L. Webber Iii

Virginia Journal of Science

Kenaf (Hibiscus cannabinus L.), a warm-season annual plant, has shown potential as an alternate source of fiber in the United States. Although preliminary research has indicated feasibility of kenaf production in Virginia, production details are lacking. Field experiments were conducted during 1995 and 1996 to determine optimal row spacing and fertilizer needs, and to compare available kenaf cultivars. Although results indicated that differences in dry matter yields from four row spacings (30, 60, 90, and 120 cm) and four rates each of N, P, and K fertilizers (50, 100, 150, and 200 kg ha-1) were not …


Fish Consumption Patterns Of Populations In Vicinities Of Lake Kastoria And Lake Pamvotis, Greece, Eugene G. Maurakis, David V. Grimes, Dimitra Bobori Oct 2005

Fish Consumption Patterns Of Populations In Vicinities Of Lake Kastoria And Lake Pamvotis, Greece, Eugene G. Maurakis, David V. Grimes, Dimitra Bobori

Virginia Journal of Science

Objectives are to establish fish consumption patterns of populations in vicinities of two lakes (Kastoria and Pamvotis) in Greece for use in the assessment of risks associated with consumption of fishes in these agri-chemically impaired lakes. Parameters measured were demographics (i.e., gender, age, weight, education level, occupation, residency), freshwater fish eating frequency, species and sizes of fishes consumed, and fish consumption habits [i.e., quantity, parts, and preparation method). All annual mean site-specific consumption rates of the four gender-age class sub-populations surveyed in vicinity of Lake Kastoria (avg. range= 0.103-0.29 kg/day) exceed those of Greece (0.066 kg/day), EU (0.068 kg/day), Spain …


Bats Of Skydusky Hollow, Bland County, Virginia, Virgil Brack Jr., Richard J. Reynolds, Wil Orndorff, Joe Zokaites, Carol Zokaites Jul 2005

Bats Of Skydusky Hollow, Bland County, Virginia, Virgil Brack Jr., Richard J. Reynolds, Wil Orndorff, Joe Zokaites, Carol Zokaites

Virginia Journal of Science

During the period 22 November 1999 – 11 October 2001, winter hibernacula surveys, spring staging/autumn swarming surveys, and summer surveys for bats were completed in caves of Skydusky Hollow, Bland County, Virginia. During winter, 12 caves were entered and 16,185 bats counted: 235 Myotis sodalis (Indiana bat), 14,475 Myotis lucifugus (little brown myotis), 12 Myotis septentrionalis (northern myotis), 7 Myotis leibii (eastern small-footed myotis), 1,441 Pipistrellus subflavus (eastern pipistrelle), and 15 Eptesicus fuscus (big brown bat). Myotis sodalis hibernated in thermally stable areas of 7 -9 ̊C. The largest concentration of M. lucifugus (n = 4,280) hibernated in an area …


A Defensin-Like Gene Expressed In The Black-Legged Tick, Ixodes Scapularis (Acari: Ixodidae), Wayne L. Hynes, S. M. Ceraul, S. M. Todd, K. C. Seguin, Daniel E. Sonenshine Jan 2005

A Defensin-Like Gene Expressed In The Black-Legged Tick, Ixodes Scapularis (Acari: Ixodidae), Wayne L. Hynes, S. M. Ceraul, S. M. Todd, K. C. Seguin, Daniel E. Sonenshine

Biological Sciences Faculty Publications

The black-legged tick Ixodes scapularis linnaeus (Acari: Ixodidae) is an important vector of microbial pathogens. Knowledge of the tick's innate immune response, particularly defensin and other antimicrobial peptides, is important for understanding how microbes survive in this tick. A defensin gene (slnA) from I. scapularis was obtained by reverse transcription-polymerase chain reaction (RT-PCR) using mRNA extracted from tissues of female ticks. RT-PCR indicated the gene was expressed in the midgut, haemocytes, and fat-body, although no evidence of a peptide was found. Sequencing a cloned cDNA fragment revealed a 225 bp open reading frame encoding a 74 amino acid pre-prodefensin, including …


The Architecture Of A Proteomic Network In The Yeast, Emad Ramadan, Christopher Osgood, Alex Pothern Jan 2005

The Architecture Of A Proteomic Network In The Yeast, Emad Ramadan, Christopher Osgood, Alex Pothern

Biological Sciences Faculty Publications

We describe an approach to clustering the yeast protein-protein inter-action network in order to identify functional modules, groups of proteins forming multi-protein complexes accomplishing various functions in the cell. We have developed a clustering method that accounts for the small-world nature of the network. The algorithm makes use of the concept of k-cores in a graph, and employs recursive spectral clustering to compute the functional modules. The computed clusters are annotated using their protein memberships into known multi-protein complexes in the yeast. We also dissect the protein interaction network into a global subnetwork of hub proteins (connected to several clusters), …


Comparison Of Phytoplankton And Autotrophic Picoplankton Populations Over A 24-Hour Period From A Pond's Surface And Subsurface Waters, Harold G. Marshall, Lubomira Burchardt, Elżbieta Wilk-Woźniak Jan 2005

Comparison Of Phytoplankton And Autotrophic Picoplankton Populations Over A 24-Hour Period From A Pond's Surface And Subsurface Waters, Harold G. Marshall, Lubomira Burchardt, Elżbieta Wilk-Woźniak

Biological Sciences Faculty Publications

Phytoplankton abundance and composition relationships within the surface layer changed over short time periods (4-6 hours) in comparison with more constant associations at 2 cm below the surface. Both strata had a diverse algal flora (>50 taxa), but no distinct neuston assemblage characterized the surface layer algal composition over the 24-hour study. The similarity between the two strata indicated the floral composition of the surface layer came from the water column algae below the surface. Chlorophytes, diatoms, cyanobacteria, and cryptophytes represented the most abundant algal categories for both strata with a total mean phytoplankton abundance of 3,566 cells ml …


Mechanical Properties Of The Integument Of The Common Gartersnake, Thamnophis Sirtalis (Serpentes: Colubridae), Gabriel Rivera, Alan H. Savitzky, Jeffrey A. Hinkley Jan 2005

Mechanical Properties Of The Integument Of The Common Gartersnake, Thamnophis Sirtalis (Serpentes: Colubridae), Gabriel Rivera, Alan H. Savitzky, Jeffrey A. Hinkley

Biological Sciences Faculty Publications

The evolution of the ophidian feeding mechanism has involved substantial morphological restructuring associated with the ability to ingest relatively large prey. Previous studies examining the morphological consequences of macrophagy have concentrated on modifications of the skull and cephalic musculature. Although it is evident that macrophagy requires highly compliant skin, the mechanical properties of the ophidian integument have received limited attention, particularly in the context of feeding. We examined mechanical properties of skin along the body axis in Thamnophis sirtalis (Colubridae). Data were collected from tensile tests and were analyzed using a multivariate analysis of variance (MANOVA) and post-hoc multiple comparison …


3,4-Methylenedioxymethamphetamine Activates Nuclear Factor- Κb, Increases Intracellular Calcium, And Modulates Gene Transcription In Rat Heart Cells, David A. Tiangco, Frank A. Lattanzio Jr., Christopher J. Osgood, Stephen J. Beebe, Julie A. Kerry, Barbara Y. Hargrave Jan 2005

3,4-Methylenedioxymethamphetamine Activates Nuclear Factor- Κb, Increases Intracellular Calcium, And Modulates Gene Transcription In Rat Heart Cells, David A. Tiangco, Frank A. Lattanzio Jr., Christopher J. Osgood, Stephen J. Beebe, Julie A. Kerry, Barbara Y. Hargrave

Biological Sciences Faculty Publications

3,4-Methylenedioxymethamphetamine (MDMA) is an illicit psychoactive drug that has gained immense popularity among teenagers and young adults. The cardiovascular toxicological consequences of abusing this compound have not been fully characterized. The present study utilized a transient transfection/dual luciferase genetic reporter assay, fluorescence confocal microscopy, and gene expression macroarray technology to determine nuclear factor-κB (NF-κB) activity, intracellular calcium balance, mitochondrial depolarization, and gene transcription profiles, respectively, in cultured rat striated cardiac myocytes (H9c2) exposed to MDMA. At concentrations of 1×10−3 M and 1×10−2 M, MDMA significantly enhanced NF-κB reporter activity compared with 0 M (medium only) control. This …


Demonstration Of Toxicity To Fish And To Mammalian Cells By Pfiesteria Species: Comparison Of Assay Methods And Strains, Joann M. Burkholder, Andrew S. Gordon, Peter D. Moeller, J. Mac Law, Kathryn J. Coyne, Alan J. Lewitus, John S. Ramsdell, Harold G. Marshall, Nora J. Deamer, S. Craig Cary, Jason W. Kempton, Steven L. Morton, Parke A. Rublee Jan 2005

Demonstration Of Toxicity To Fish And To Mammalian Cells By Pfiesteria Species: Comparison Of Assay Methods And Strains, Joann M. Burkholder, Andrew S. Gordon, Peter D. Moeller, J. Mac Law, Kathryn J. Coyne, Alan J. Lewitus, John S. Ramsdell, Harold G. Marshall, Nora J. Deamer, S. Craig Cary, Jason W. Kempton, Steven L. Morton, Parke A. Rublee

Biological Sciences Faculty Publications

Toxicity and its detection in the dinoflagellate fish predators Pfiesteria piscicida and Pfiesteria shumwayae depend on the strain and the use of reliable assays. Two assays, standardized fish bioassays (SFBs) with juvenile fish and fish microassays (FMAs) with larval fish, were compared for their utility to detect toxic Pfiesteria. The comparison included strains with confirmed toxicity, negative controls (noninducible Pfiesteria strains and a related nontoxic cryptoperidiniopsoid dinoflagellate), and P. shumwayae strain CCMP2089, which previously had been reported as nontoxic. SFBs, standardized by using toxic Pfiesteria (coupled with tests confirming Pfiesteria toxin) and conditions conducive to toxicity expression, reliably detected …