Dairy Science Commons^™

Prevention Of Viral Drug Resistance By Novel Combination Therapy, W. F. Mangel, M. T. Brown, M. L. Baniecki, Dale L. Barnard, W. J. Mcgrath

Animal, Dairy, and Veterinary Science Faculty Publications

A new form of antiviral clinical therapy is proposed in which three different drugs are administered against three different targets on the same virus-coded protein. If the physiological functions of the three different target sites are not independent of each other, then a mutation conferring drug resistance at one site may alter the physiological functions at the other sites and further drug resistance may not arise. The adenovirus proteinase, with its two cofactors that act synergistically on enzyme activity, may be a good model system within which to test the efficacy of this form of combination therapy.

Genvir, Dale L. Barnard

Animal, Dairy, and Veterinary Science Faculty Publications

Flamel Technologies is developing Genvir (formerly known as Viropump), a twice-daily controlled-release formulation of aciclovir, for potential use in the treatment of herpes simplex virus and varicella zoster virus infections. Genvir utilizes Flamel's proprietary Micropump technology, a microparticle-based drug delivery system designed to extend the time of absorption of drugs in the small intestine. The drug shows a comparable therapeutic efficacy to valaciclovir and famciclovir (both GlaxoSmithKline) [313393]. Phase III trials have been completed [302829]. In August 2000, Flamel filed for regulatory approval for the treatment of herpes in France, as a prelude to a pan-European approval [378641] and is …

Inhibition Of Measles Virus Replication By 5'-Norcarbocyclic Nucleoside Analogs, Dale L. Barnard, V. Stowell, K. L. Seley, V. R. Hegde, R. Subha, P. Rajappan, S. W. Schneller, Donald F. Smee, R. W. Sidwell

Animal, Dairy, and Veterinary Science Faculty Publications

Despite intense efforts to increase vaccine coverage, measles virus (MV) still causes significant morbidity and mortality in the world, sometimes as the result of severe, chronic, lethal disease. In an effort to develop therapies to supplement immunization strategies, a number of 5′-nor carbocyclic adenosine analogues were evaluated for anti-MV activity in CV-1 monkey kidney cells. Of those compounds tested, those either unsubstituted at C4 or possessing a hydroxyl, azido or amino substituent at that position were the most active, with particularly significant inhibition of MV, strain Chicago-1. The EC50 values against this strain ranged from100 mg/ml in actively growing and …

2000 Semi-Annual Report, Various Authors

Annual Reports

No abstract provided.

1999 Annual Report, Various Authors

Annual Reports

No abstract provided.

2000 Annual Report, Various Authors

Annual Reports

No abstract provided.

Pegasys, Dale L. Barnard

Animal, Dairy, and Veterinary Science Faculty Publications

No abstract provided.

1998 Annual Report, Various Authors

Annual Reports

No abstract provided.

13th Biennial Cheese Industry Conference, Various Authors

Cheese Industry Conference

No abstract provided.

The Influence Of Connective Tissue In Meat Tenderness (A Histological And Comparative Study), Rachael Anne Adams

Undergraduate Honors Capstone Projects

There are several means of determining the tenderness of meat. Recently, a great deal of effort has been focused on connective tissue, or collagen, as a measure of tenderness. These foci have included biochemical determination of collagen types, the ratios of soluble and insoluble collagen, changes of collagen with age, and abundance of collagen.

The purpose of this study is to evaluate the abundance of collage as a means of differentiating levels of tenderness , using histology. Steaks taken from the bovine tenderloin, inside round, and bottom round (eye) were observed histologically using special stains for connective tissue. This method, …

The Role Of Ammonia In Reproductive Inefficiency In High-Producing Dairy Cows Fed Excess Rumen Degraded Protein, Douglas Scott Hammon

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The role of ammonia in reproductive inefficiency in early lactation dairy cows was studied in a series of experiments designed to determine the concentrations of ammonia in normal bovine follicular fluid (bFF), to determine the effects of ammonia on the bovine embryo during specific stages of development, and to test the hypothesis that elevated plasma urea nitrogen concentration is associated with elevated ammonia and urea nitrogen concentrations in the reproductive fluids.

In the first study, ammonia concentration in different size follicles and the effect of ammonia during in vitro maturation on embryo development were determined. Ammonia concentration in the bFF …

1998 Annual Meeting, Various Authors

Annual Reports

No abstract provided.

Selecting Linear-Score Distributions For Modeling Milk-Culture Results, H. G. Allore, David J. Wilson, H. N. Erb, P. A. Oltenacu

Animal, Dairy, and Veterinary Science Faculty Publications

The data for this cross-sectional retrospective study are from surveys of 65 dairy-cattle herds in central New York, USA sampled between February, 1993 and March, 1995. The objective was to identify probability distributions of logarithmically transformed somatic-cell counts (linear score) for use in a simulation model of mastitis and milk quality. Probability density functions were estimated using maximum-likelihood estimators for the linear score of individual-cow composite milk samples culture negative and culture positive for the pathogens Streptococcus agalactiae, Streptococcus non-agalactiae, Staphylococcus aureus, and coagulase-negative staphylococci for the complete dataset and by bulk-tank somatic-cell count group (< 500 000, ≥ 500 000 SCC/ml). Based on the rankings of three goodness-of-fit tests (Anderson-Darling, Kolmogorov-Smirnov and x^{2 …}

Swiss Cheese Consortium Project Report, Various Authors

Other Documents

No abstract provided.

1997 Annual Report, Various Authors

Annual Reports

No abstract provided.

Western Dairy Center Project Report, Bruce L. Geller

Other Documents

No abstract provided.

Acyclic Phosphonomethylether Nucleosideinhibitors Of Respiratory Viruses, Dale L. Barnard, N. Bischofberger, C. U. Kim, J. H. Huffman, R. W. Sidwell, J. P. Dougherty, W. Lew, M. A. Williams, W. Yang

Animal, Dairy, and Veterinary Science Faculty Publications

A series of acyclic phosphonomethylether nucleosides were synthesized and then evaluated for inhibitory activity against respiratory viruses of clinical significance using CPE inhibition, neutral red uptake and virus yield reduction assays. Of the 20 compounds synthesized, none significantly inhibited influenza A or B viruses or respiratory syncytial virus strains A2, Long or 18537; the selective indices (SI) were less than 10. A new compound, GS-2128 (2R, 5R-9-[2,5-dihydro-5-(phosphonomethoxy)-2-furanyl]adenine; D4API), selectively inhibited adenovirus 5 (SI>10) as did GS-0577 (9-(3-hydroxy-2-phosphonylmethoxypropyl)-adenine; HPMPA) and GS-0504 [(S)-1-[3-hydroxy-2-(phosphonylmethoxypropyl)]-cytosine; HPMPC]. The 50% effective concentrations (EC50) ranged from 8-100 μg mL-1 and 50% cell inhibitory concentrations (CC50) from …

Western Dairy Center Project Report, Donald J. Mcmahon, Jeffery Broadbent, Craig Oberg

Other Documents

No abstract provided.

Bovine Mastitis Pathogens In New York Andpennsylvania: Prevalence And Effects On Somatic Cell Count And Milk Production, David J. Wilson, R. N. Gonzalez, H. H. Das

Animal, Dairy, and Veterinary Science Faculty Publications

Milk samples were collected from 108,312 dairy cows during 1601 farm visits made between January 1991 and June 1995. The herd visits were made by personnel from the Central Laboratory of the Quality Milk Promotion Services at Cornell University (Ithaca, NY) to farms located in central New York and northern Pennsylvania. Dairy Herd Improvement Association records were available for 32,978 cows in 327 herds. Intramammary infections, as defined by positive milk cultures, were present in 48.5% of all cows and in 36.3% of cows in herds enrolled in the Dairy Herd Improvement Association. Over 75% of the intramammary infections were …

In Vitro Influenza Virus-Inhibitory Effects Of A Series Of Germanium- Orsilicon-Centered Polyoxometalates, J. H. Huffman, R. W. Sidwell, Dale L. Barnard, A. Morrison, M. I. Otto, C. L. Hill, R. F. Schinazi

Animal, Dairy, and Veterinary Science Faculty Publications

A series of germanium- or silicon-centred heteropolytungstates (polyoxometalates) with the Barrel, Keggin or double Keggin structure were evaluated in vitro for their effects against influenza A (IV-A) and B (IV-B) viruses. Their 50% effective concentrations (EC₅₀) against recent isolates of IV-A (H1 N1 ) and IV-B ranged from 0.1 to 7.8 μM; against IV-A (H3N2), the EC₅₀ concentrations were often 10-fold higher. Recent clinical isolates of IV-A were generally more susceptible to these antiviral effects than older, laboratory-adapted strains. These experiments used inhibition of viral CPE in MDCK cells as determined microscopically and by Neutral Red (NR) …

12th Biennial Cheese Industry Conference, Various Authors

Cheese Industry Conference

No abstract provided.

The Influence Of Fluorescent Light On The Development Of In Vitro Fertilized Bovine Oocytes, Jared Bunch

Undergraduate Honors Capstone Projects

Advances in in vitro fertilization and in vitro culture techniques have allowed considerable progress in identifying physiological requirements of mammalian embryos. Parrish et al. (11) reported a major breakthrough on in vitro fertilization when his group identified heparin as an important factor for the capacitation of spermatozoa. Capacitation is necessary for fertilization of matured oocytes. During the precoculture era of embryos (prior to 1980), the development of early preirnplantation embryos was vary limited regardless of medium, medium supplement, gas atmosphere, osmolarity or pH used (7). Coculture techniques of fertilized oocytes using somatic cells during in vitro production, particularly of bovine …

The Use Of The Xylose Tolerance Test As An Indicator Of Malabsorption In Cryptosporidiosis, James A. Evans

Undergraduate Honors Capstone Projects

Cryptosporidium parvum, a protozoan parasite infecting epithelial cells lining the intestinal tract of animals and humans, causes fulminate diarrhea and malabsorption of essential nutrients following damage to the cellular brush border. The present study was undertaken to evaluate the use of a pentose sugar (D-xylose) in a standardized tolerance test as an indicator of malabsorption due to cryptosporidiosis in the dexamethasone (DEX)-immunosuppressed adult C57BL/6N mouse model. One group containing 14 experimental mice (in addition to appropriate control groups) was immunosuppressed using daily intraperitoneal injections of DEX (125 μg/mouse). On day 7 postimmunosuppression, mice were inoculated orally with 104 C. …

1996 Annual Report, Various Authors

Annual Reports

No abstract provided.

1995 Annual Report, Various Authors

Annual Reports

No abstract provided.

Lowfat Cheese Research Conference Proceedings, Various Authors

Other Documents

No abstract provided.

1994 Annual Report, Various Authors

Annual Reports

No abstract provided.

1993 Annual Report, Various Authors

Annual Reports

No abstract provided.

Biological Action Of Mycotoxins, Roger A. Coulombe Jr.

Animal, Dairy, and Veterinary Science Faculty Publications

Mycotoxins are ubiquitous, mold-produced toxins that contaminate a wide variety of foods and feeds. Ingestion of mycotoxins cause a range of toxic responses, from acute toxicity to long-term or chronic health disorders. Some mycotoxins have caused outbreaks of human toxicoses, and at least one mycotoxin, aflatoxin B1, is a presumed human hepatocarcinogen. As part of a comprehensive effort to curtail the adverse health effects posed by mycotoxins, substantial research has been conducted to determine the mechanism of action of mycotoxins in animals. This review presents some of the current knowledge on the biological action of four diverse classes of mycotoxins—aflatoxin …

Selection And Development Of Replacement Beef Heifers, Norris J. Stenquist, James A. Bennett

Archived Agriculture Publications

Selection, development, management, and nutrition of heifer calves to be used as herd replacements are key factors in maintaining beef herd productivity. A cow/calf producer, to be successful, must have some goals or criterion for heifer selection and development. For practically all Utah cattle production areas, economics dictate that heifers must calve as two-year-olds. Heifers must be properly developed and managed to permit this. The following factors are important: Heifers should 1) become pregnant in the first 25 days of the breeding season, 2) give birth to a live calf with little or no calving difficulty, 3) raise a calf …