Beef Science Commons^™

Evaluation Of Salmonella Biofilm Cell Transfer From Common Food Contact Surfaces To Beef Products, Rong Wong, David A. King, Norasak Kalchayanand

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Meat contamination by Salmonella enterica is a serious public health concern. Available data have suggested that biofilm formation at processing plants and contaminated contact surfaces might contribute to meat contamination. Because transfer from contact surfaces to food products via direct contact has been deemed the most common bacteria transmission route that can lead to contamination, we evaluated the effect of Salmonella biofilm forming ability, contact surface material, and beef surface tissue type on Salmonella biofilm transfer from hard surfaces to beef products. Salmonella biofilms developed on the common contact surfaces stainless steel (SS) and polyvinylchloride (PVC) were transferred consecutively via …

Hotline Number To Reach And Offer Agricultural Information To Plain Anabaptists During The Covid-19 Workplace Closures, Emily Shoop, Carly Becker, Nathan Glenn Briggs, Danielle Smarsh

Journal of Amish and Plain Anabaptist Studies

The Penn State Extension Animal Systems Team developed a toll-free hotline phone number to convey short educational messages to maintain contact and outreach to Pennsylvania’s animal producing Amish, Mennonite, and other plain Anabaptist populations. During the COVID-19 pandemic, Penn State Extension’s programming went largely online via webinars, online courses, and emails. This change in programming excluded a large sector of Pennsylvania farmers who do not use or have access to the internet and, as such, were liable to miss important best management practice reviews and timely updates in animal agriculture. The Animal Systems Hotline offered callers the choice to listen …

Relationship Of Winter Hair Coat Shedding Ability Of Purebred Angus Dams With Mineral Status, Apparent Forage Digestibility, Uterine Artery Hemodynamics, And Calf Growth And Development, Randy Hunter Burnett

Theses and Dissertations

Winter hair coat shedding ability in southern U.S. cattle production has previously been associated with differences in adjusted birth and weaning weights of calves from dams that shed by June or later (Gray et al., 2011; Cauble et al., 2019). This study was developed to research possible avenues of why these relationships exist with winter hair coat shedding ability. The primary objectives of the following experiments were to: 1) evaluate how mineral status relates to winter hair coat shedding ability, 2) evaluate how winter hair coat shedding ability relates to apparent digestibility of forage, 3) evaluate winter hair coat shedding …

Transcriptome Profiles Of The Skeletal Muscle Of Mature Cows During Feed Restriction And Realimentation, Hannah C. Cunningham-Hollinger, Larry A. Kuehn, Kristi M. Cammack, Kristin E. Hales, William T. Oliver, Matthew S. Crouse, Celine Chen, Harvey C. Freetly, Amanda K. Lindholm-Perry

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Objective: Realimentation can compensate for weight loss from poor-quality feedstuffs or drought. Mature cows fluctuate in body weight throughout the year due to nutrient availability. The objective of this study was to determine whether cows that differ in weight gain during realimentation also differ in the abundance of transcripts for enzymes associated with energy utilization in skeletal muscle. Mature cows were subjected to feed restriction followed by ad libitum feed. Skeletal muscle transcriptome expression differences during the two feeding periods were determined from cows with greater (n = 6) and less (n = 6) weight gain during the ad libitum …

Cattle Industry Funding Scheme Annual Report 2020/2021, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity published reports

In June 2010 the Cattle Industry Funding Scheme (IFS) was introduced to address biosecurity threats relevant to the Western Australian (WA) cattle industry. The Scheme was established under the Biosecurity and Agriculture Management Act 2007 to enable cattle producers to identify the pest and disease priorities at a whole-of-industry level and to raise funds for activities to address these priorities.

There are currently three Industry Funding Schemes in operation. In addition to the Cattle IFS, there is a Sheep and Goat IFS and a Grains, Seeds and Hay IFS. The three IFSs operate in a similar manner.

The purpose of …

Management Of Late Summer Planted Annual Forages For Grazing And The Impacts Of Novel Sweet Bran Plus Products On Performance And Carcass Characteristics Of Beef Finishing Steers, Devin Anthony Jakub

Department of Animal Science: Dissertations, Theses, and Student Research

Two separate grazing studies were conducted to evaluate: 1) the effects of rapeseed inclusion into late summer planted oats on the performance of growing steers during late fall and winter (Exp. 1) and 2) the effects of forage allocation on forage utilization and performance of steers grazing a late summer planted oat-rapeseed mix (Exp. 2). Variation in the corn wet milling process can result in negative effects on animal performance, nonetheless, there remains interest in further exploration and refinement of corn wet milling byproducts to achieve optimal byproduct compositions and cattle performance. Thus, another experiment was conducted to evaluate the …

Impact Of Wood-Sourced Biochar On Carbon And Nitrogen Capture In Beef Feedlot Systems, Jessica L. Sperber

Department of Animal Science: Dissertations, Theses, and Student Research

A feedlot growing and finishing experiment evaluated the effect of including pine-sourced biochar at 0.8 (growing) and 1.0% (finishing) of dietary DM on steer performance, carcass characteristics, and greenhouse gas (GHG) production (Exp 1). Two nutrient mass balance experiments were conducted during winter and summer seasons to evaluate the effect of spreading unprocessed red cedar biochar on the feedlot pen surface on manure nutrient capture and cattle performance (Exp 2). In Exp. 1, the inclusion of biochar in the growing diet did not impact steer performance. The inclusion of biochar in the finishing diet significantly reduced intake and gain, resulting …

Evaluation Of Cattle Management For Systems With Limited Perennial Pasture, Morgan Grabau

Department of Animal Science: Dissertations, Theses, and Student Research

Due to the conversion of perennial pasture and hay land to cropland in the western corn belt region, alternative methods of cow/calf management are being used to decrease reliance on perennial forages. These include grazing corn residue and feeding cow/calf pairs in confinement. Previous research has shown that limit-feeding a diet of low-quality forage and energy-dense co-products to cows in confinement is cost-effective. However, this strategy may limit the intake of the young calf and thus their performance. A study was conducted to evaluate the impacts of two alternative calf management strategies when pairs are in confinement, early weaning or …

Considerations And Best Practices In Animal Science 16s Ribosomal Rna Gene Sequencing Microbiome Studies, Margaret D. Weinroth, Aeriel D. Belk, Chris Dean, Noelle Noyes, Dana K. Dittoe, Michael J. Rothrock Jr, Steven C. Ricke, Phillip R. Myer, Madison T. Henniger, Gustavo A. Ramírez, Brian B. Oakley, Katie Lynn Summers, Asha M. Miles, Taylor B. Ault-Seay, Zhongtang Yu, Jessica L. Metcalf, James E. Wells

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Microbiome studies in animal science using 16S rRNA gene sequencing have become increasingly common in recent years as sequencing costs continue to fall and bioinformatic tools become more powerful and user-friendly. The combination of molecular biology, microbiology, microbial ecology, computer science, and bioinformatics—in addition to the traditional considerations when conducting an animal science study—makes microbiome studies sometimes intimidating due to the intersection of different fields. The objective of this review is to serve as a jumping-off point for those animal scientists less familiar with 16S rRNA gene sequencing and analyses and to bring up common issues and concerns that arise …

Twenty-Four-Month Longitudinal Study Suggests Little To No Horizontal Gene Transfer In Situ Between Third-Generation Cephalosporin-Resistant Salmonella And Third-Generation Cephalosporin-Resistant Escherichia Coli In A Beef Cattle Feedyard, John W. Schmidt, Sarah A. Murray, Aaron M. Dickey, Tommy L. Wheeler, Dayna M. Harhay, Terrance M. Arthur

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Third-generation cephalosporins (3GCs) are preferred treatments for serious human Salmonella enterica infections. Beef cattle are suspected to contribute to human 3GC-resistant Salmonella infections. Commensal 3GC-resistant Escherichia coli are thought to act as reservoirs of 3GC resistance because these strains are isolated more frequently than are 3GC-resistant Salmonella strains at beef cattle feedyards. During each of 24 consecutive months, four samples of pen surface material were obtained from five pens (N = 480) at a Nebraska feedyard to determine to the contribution of 3GC-resistant E. coli to the occurrence of 3GC-resistant Salmonella. Illumina whole genome sequencing was performed, and …

Adaptive Selection Signatures In River Buffalo With Emphasis On Immune And Major Histocompatibility Complex Genes, Yan Ren, Callum Macphillamy, Thu Hien To, Timothy P. L. Smith, John L. Williams, Wai Yee Low

Roman L. Hruska U.S. Meat Animal Research Center: Reports

River buffalo is an agriculturally important species with many traits, such as disease tolerance, which promote its use worldwide. Highly contiguous genome assemblies of the river buffalo, goat, pig, human and two cattle subspecies were aligned to study gene gains and losses and signs of positive selection. The gene families that have changed significantly in river buffalo since divergence from cattle play important roles in protein degradation, the olfactory receptor system, detoxification and the immune system. We used the branch site model in PAML to analyse single-copy orthologs to identify positively selected genes that may be involved in skin differentiation, …

Evaluating Large Spontaneous Deletions In A Bovine Cell Line Selected For Bovine Viral Diarrhea Virus Resistance, Aspen M. Workman, Michael P. Heaton, Dennis A. Webster, Gregory P. Harhay, Theodore S. Kalbfleisch, Timothy P.L. Smith, Shollie M. Falkenberg, Daniel F. Carlson, Tad S. Sonstegard

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Bovine viral diarrhea virus’s (BVDV) entry into bovine cells involves attachment of virions to cellular receptors, internalization, and pH-dependent fusion with endosomal membranes. The primary host receptor for BVDV is CD46; however, the complete set of host factors required for virus entry is unknown. The Madin-Darby bovine kidney (MDBK) cell line is susceptible to BVDV infection, while a derivative cell line (CRIB) is resistant at the level of virus entry. We performed complete genome sequencing of each to identify genomic variation underlying the resistant phenotype with the aim of identifying host factors essential for BVDV entry. Three large compound deletions …

Moraxella Species Associated With Infectious Bovine Keratoconjunctivitis: Detection, Characterization, And Immunity, Matthew M. Hille

School of Veterinary and Biomedical Sciences: Dissertations, Theses, and Student Research

Infectious bovine keratoconjunctivitis (IBK) represents the most common ocular disease of cattle. Moraxella bovis (M. bovis) is the only bacteria proven to cause IBK under experimental conditions. A closely related bacteria, Moraxella bovoculi (M. bovoculi) is cultured from IBK lesions more frequently than M. bovis, and is suspected to cause IBK, although a causal relationship between M. bovoculi and IBK has not been confirmed experimentally. Two distinct genotypes were recently characterized in M. bovoculi based on whole genome sequencing. Genotype 1 M. bovoculi appears to represent a potential pathogen …

Predicting Carcass Cut Yields In Cattle From Digital Images Using Artificial Intelligence, Darragh Matthews, Thierry Pabiou, Ross D. Evans, Christian Beder, Aengus Daly

Department of Mathematics Publications

Deep Learning (DL) has proven to be a successful tool for many image classification problems but has yet to be applied to carcass images. The aim of this study was to train DL models to predict carcass cut yields and compare predictions to more standard machine learning (ML) methods. Three approaches were undertaken to predict the grouped carcass cut yields of Grilling cuts and Roasting cuts from a large dataset of 54,598 and 69,246 animals respectively. The approaches taken were (1) animal phenotypic data used as features for a range of ML algorithms, (2) carcass images used to train Convolutional …

Localization Of Kisspeptin, Nkb, And Nk3r In The Hypothalamus Of Gilts Treated With The Progestin Altrenogest, Ashley N. Lindo, Jennifer F. Thorson, Michelle N. Bedenbaugh, Richard B. Mccosh, Justin A. Lopez, Samantha A. Young, Lanny J. Meadows, Elizabeth C. Bowdridge, Chrysanthi Fergani, Bradley A. Freking, Michael N. Lehman, Stanley M. Hileman, Clay A. Lents

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Mechanisms in the brain controlling secretion of gonadotropin hormones in pigs, particularly luteinizing hormone (LH), are poorly understood. Kisspeptin is a potent LH stimulant that is essential for fertility in many species, including pigs. Neurokinin B (NKB) acting through neurokinin 3 receptor (NK3R) is involved in kisspeptin-stimulated LH release, but organization of NKB and NK3R within the porcine hypothalamus is unknown. Hypothalamic tissue from ovariectomized (OVX) gilts was used to determine the distribution of immunoreactive kisspeptin, NKB, and NK3R cells in the arcuate nucleus (ARC). Almost all kisspeptin neurons coexpressed NKB in the porcine ARC. Immunostaining for NK3R was distributed …

Relationship Of Molecular Breeding Value For Beef Tenderness With Heifer Traits Through Weaning Of Their First Calf, Robert A. Cushman, Gary L. Bennett, Richard G. Tait, Anthony K. Mcneel, Eduardo Casas, Timothy P.L. Smith, Harvey C. Freetly

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Polymorphisms in μ-calpain (CAPN1) that beneficially associate with beef tenderness are reported to antagonistically associate with calving day in beef heifers and post-partum interval to estrus in beef cows. We, therefore, hypothesized that a molecular breeding value for slice shear force, calculated based on CAPN1 and calpastatin (CAST) genotypes, would demonstrate an antagonistic relationship between genomically predicted slice shear force and ordinal calving date in replacement beef heifers. A secondary objective of this study was to evaluate the association of a polymorphism in diacylglycerol O-acyltransferase (DGAT1) with reproductive traits in beef heifers. One hundred eighty-seven MARC III heifers (¼ Angus, …

Validation Of High-Resolution Melting Assays For The Detection Of Virulent Strains Of Escherichia Coli O26 And O111 In Beef And Pork Enrichment Broths, Frank J. Velez, Joseph M. Bosilevac, Prashant Singh

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Extensive validation of diagnostic assays using widely collected surveillance samples is critical for developing pathogen detection assays. The detection of potentially virulent E. coli strains is critical to the red meat industry. We previously developed two high-resolution melting (HRM) assays for detecting potentially virulent and avirulent E. coli O26 and O111 strains. Assays were validated using enriched beef (n = 36) and pork (n = 36) samples collected as part of a U.S. federal regulatory surveillance program. Data from this study showed more than 90% sensitivity and specificity for both the HRM assays, demonstrating suitability for the red meat industry …

Genomic Analysis Of Shiga Toxin-Containing Escherichia Coli O157:H7 Isolated From Argentinean Cattle, Ariel Amadio, James L. Bono, Matías Irazoqui, Mariano Larzábal, Wanderson Marques Da Silva, María Florencia Eberhardt, Nahuel A. Riviere, David Gally, Shannon D. Manning, Angel Cataldi

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Cattle are the main reservoir of Enterohemorrhagic Escherichia coli (EHEC), with O157:H7 the distinctive serotype. EHEC is the main causative agent of a severe systemic disease, Hemolytic Uremic Syndrome (HUS). Argentina has the highest pediatric HUS incidence worldwide with 12–14 cases per 100,000 children. Herein, we assessed the genomes of EHEC O157:H7 isolates recovered from cattle in the humid Pampas of Argentina. According to phylogenetic studies, EHEC O157 can be divided into clades. Clade 8 strains that were classified as hypervirulent. Most of the strains of this clade have a Shiga toxin stx2a-stx2c genotype. To better understand the molecular bases …

Heat Stress And Reproduction In Beef Cattle, Reganne K. Briggs, Kerry A. Rood

All Current Publications

Reproduction could be considered the most important production trait on a cow-calf operation. Economic outputs are sustained when cattle rebreed and calve every year in a timely manner. However, when cattle are unable to rebreed efficiently, profits may decrease for producers. It is well known that heat stress can negatively impact reproduction in cattle (Jordan, 2003). The Intermountain West has been impacted by drought and increasing temperatures, both of which can impact reproduction rates in cattle. This fact sheet helps producers understand the consequences of heat stress on cattle reproduction and how they can manage reproduction during increasing temperatures.

Characterisation Of Early Positive Mcr‐1 Resistance Gene And Plasmidome In Escherichia Coli Pathogenic Strains Associated With Variable Phylogroups Under Colistin Selection, Guerrino Macori, Scott V. Nguyen, Ankita Naithani, Daniel Hurley, Li Bai, Farid El Garch, Frédérique Woehrlé, Christine Miossec, Benjamin Roques, Peadar O’Gaora, James L. Bono, Séamus Fanning

Roman L. Hruska U.S. Meat Animal Research Center: Reports

An antibiotic susceptibility monitoring programme was conducted from 2004 to 2010, re-sulting in a collection of 143 Escherichia coli cultured from bovine faecal samples (diarrhoea) and milk‐aliquots (mastitis). The isolates were subjected to whole‐genome sequencing and were distrib-uted in phylogroups A, B1, B2, C, D, E, and G with no correlation for particular genotypes with pathotypes. In fact, the population structure showed that the strains belonging to the different phy-logroups matched broadly to ST complexes; however, the isolates are randomly associated with the diseases, highlighting the necessity to investigate the virulence factors more accurately in order to identify the mechanisms …

Beef Cow Operations During Drought: Dealing With More Than Dry Conditions, Matthew Stockton

Cornhusker Economics

The most recent drought conditions have grown to cover a large portion of the west and the central United States, as shown in Figure 1. Those depending on rain continue to watch and hope that conditions will change, while implementing and considering various strategies to preserve their livelihoods. Those who have been in the business for years have seen other occasions when dry conditions limited forage production and created hardship. Each occurrence has varying outcomes, depending on the many factors faced by individuals. While drought may produce the same dry conditions for many, its effects are individualized. There are as …

Primary Industries Development Research Highlights 2021, Department Of Primary Industries And Regional Development, Tim Scanlon

Books & book chapters

The Primary Industries Development Research Highlights 2021 showcases the breadth and depth of the Department of Primary Industries and Regional Development’s research and development activities over the past several years.

Stories featured in Research Highlights 2021 stem from about 60 (of 140) current and recently-completed projects undertaken by the Department of Primary Industries and Regional Development’s (DPIRD) 1100 scientists, technical experts and economists throughout the State.

Explore our Research Highlights 2021.

The publication demonstrates the innovative and applicable research that DPIRD and its collaborators and investment partners deliver to Western Australia.

Download the Research Highlights 2021 here. Alternatively, …

Meat Tenderness: Advances In Biology, Biochemistry, Molecular Mechanisms And New Technologies, Robyn D. Warner, Tommy L. Wheeler, Minh Ha, Xin Li, Alaa El-Din Bekhit, James Morton, Rozita Vaskoska, Frank R. Dunshea, Rui Lui, Peter Purslow, Wangang Zhang

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Meat tenderness is an important quality trait critical to consumer acceptance, and determines satisfaction, repeat purchase and willingness-to-pay premium prices. Recent advances in tenderness research from a variety of perspectives are presented. Our understanding of molecular factors influencing tenderization are discussed in relation to glycolysis, calcium release, protease activation, apoptosis and heat shock proteins, the use of proteomic analysis for monitoring changes, proteomic biomarkers and oxidative/nitrosative stress. Each of these structural, metabolic and molecular determinants of meat tenderness are then discussed in greater detail in relation to animal variation, postmortem influences, and changes during cooking, with a focus on recent …

Behavioral And Productive Parameters Of Lactating Dairy Cows As Indicators Of The Early Detection Of Metabolic And Digestive Diseases, Juan Manuel Vergara

International Programs

No abstract provided.

Pilot-Scale H2S And Swine Odor Removal System Using Commercially Available Biochar, Kyoung S. Ro, Brian Woodbury, Mindy Spiehs, Ariel A. Szogi, Philip J. Silva, Okhwa Hwang, Sungback Cho

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Although biochars made in laboratory seem to remove H2S and odorous compounds effectively, very few studies are available for commercial biochars. This study evaluated the efficacy of a commercial biochar (CBC) for removing H2S and odorous volatile organic compounds (VOCs). We found that the well-known Ergun equation may not be adequate in predicting pressure drop for properly sizing a ventilation system. The H2S breakthrough adsorption capacity of the CBC was 2.51 mg/g under humid conditions, which was much higher than that for dry conditions. The breakthrough capacity increased with the influent concentration of H2S. The efficacy of a pilot-scale biochar …

The Ovarian Follicle Of Ruminants: The Path From Conceptus To Adult, Jennifer L. Juengel, Robert A. Cushman, Joëlle Dupont, Stéphane Fabre, Richard G. Lea, Graeme B. Martin, Francesca Mossa, Janet L. Pitman, Christopher A. Price, Peter Smith

Roman L. Hruska U.S. Meat Animal Research Center: Reports

This review resulted from an international workshop and presents a consensus view of critical advances over the past decade in our understanding of follicle function in ruminants. The major concepts covered include: (1) the value of major genes; (2) the dynamics of fetal ovarian development and its sensitivity to nutritional and environmental influences; (3) the concept of an ovarian follicle reserve, aligned with the rise of anti-Müllerian hormone as a controller of ovarian processes; (4) renewed recognition of the diverse and important roles of theca cells; (5) the importance of follicular fluid as a microenvironment that determines oocyte quality; (6) …

Evolution Of The Sheep Industry And Genetic Research In The United States: Opportunities For Convergence In The Twenty-First Century, J. W. Thorne, B. M. Murdoch, B. A. Freking, R. R. Redden, Thomas W. Murphy, J. B. Taylor, Harvey D. Blackburn

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The continuous development and application of technology for genetic improvement is a key element for advancing sheep production in the United States. The US sheep industry has contracted over time but appears to be at a juncture where a greater utilization of technology can facilitate industry expansion to new markets and address inefficiencies in traditional production practices. Significant transformations include the increased value of lamb in relation to wool, and a downtrend in large-scale operations but a simultaneous rise in small flocks. Additionally, popularity of hair breeds not requiring shearing has surged, particularly in semi-arid and subtropical US environments. A …

Economic Contribution Of The Agricultural Sector To The Arkansas Economy In 2019, Leah English, Jennie Popp, Wayne Miller

AAES Research Reports and Research Bulletins

Agriculture and associated agricultural activities are major contributors to the Arkansas economy. Agriculture is defined as the sum of agricultural production and processing activities, unless otherwise specified, and includes crop and animal production and processing, agricultural support industries, forestry and forest products, and textile goods. Agriculture contributes to the economy through direct agricultural production and value-added processing, leading to economic activity in other parts of the economy.

This report is the fourteenth in a series that examines agriculture’s economic contribution to the Arkansas economy. The total economic contribution of agriculture (direct, indirect, and induced effects) on value added, employment, and …

Influence Of Estradiol On Bovine Trophectoderm And Uterine Gene Transcripts Around Maternal Recognition Of Pregnancy, Emmalee J. Northrop-Albrecht, Jerica J.J. Rich, Robert A. Cushman, Runan Yao, Xijin Ge, George A. Perry

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Embryo survival and pregnancy success is increased among animals that exhibit estrus prior to fixed time-artificial insemination, but there are no differences in conceptus survival to d16. The objective of this study was to determine effects of preovulatory estradiol on uterine transcriptomes, select trophectoderm (TE) transcripts, and uterine luminal fluid proteins. Beef cows/heifers were synchronized, artificially inseminated (d0), and grouped into either high (highE2) or low (lowE2) preovulatory estradiol. Uteri were flushed (d16); conceptuses and endometrial biopsies (n = 29) were collected. RNA sequencing was performed on endometrium. Real-time polymerase chain reaction (RT-PCR) was performed on TE (n = 21) …

Low-Oxygen Dry Aging Of Beef, Joseph A. Sonderman

Department of Animal Science: Dissertations, Theses, and Student Research

The objective of this research project was to determine the effects of removing oxygen from the beef dry aging process. Boneless upper 2/3 Choice beef strip loins (n = 18) were randomly assigned to three aging treatments: wet aging, traditional (aerobic) dry aging, and low-oxygen dry aging. All treatments were aged for 41 days at 2 ± 1°C. The dry-aged treatments were held at 50% relative humidity (RH) with a fan speed of 2,200 revolutions per minute (RPM). Low-oxygen dry-aged samples were aged in chambers housed within an oxygen impermeable film bubble. After aging, yield was determined for the …