Beef Science Commons^™

Complete Closed Genome Sequence Of The Extremely Heat- Resistant Strain Escherichia Coli Aw1.7, Manita Guragain, James L. Bono, Joseph M. Bosilevac

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Escherichia coli isolate AW1.7 is an extremely heat-resistant bacterium and has been widely used as a reference strain in extreme heat resistance studies for almost a decade. Here, we report its complete closed genome sequence.

Using Genomics To Measure Phenomics: Repeatability Of Bull Prolificacy In Multiple-Bull Pastures, Gary L. Bennett, John W. Keele, Larry A. Kuehn, Warren M. Snelling, Robert A. Cushman, Tara G. Mcdaneld, Aaron M. Dickey, Darrell Light

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Phenotypes are necessary for genomic evaluations and management. Sometimes genomics can be used to measure phenotypes when other methods are difficult or expensive. Prolificacy of bulls used in multiple-bull pastures for commercial beef production is an example. A retrospective study of 79 bulls aged 2 and older used 141 times in 4–5 pastures across 4 years was used to estimate repeatability from variance components. Traits available before each season’s use were tested for predictive ability. Sires were matched to calves using individual genotypes and evaluating exclusions. A lower-cost method of measuring prolificacy was simulated for five pastures using the bulls’ …

Production Performance Of Cows Raised With Different Postweaning Growth Patterns, Harvey C. Freetly, Robert A. Cushman, Gary L. Bennett

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The period of heifer development is a relatively small fraction of a cow's life; however, her pattern of growth may have permanent effects on her productivity as a cow. We hypothesized that altering the growth pattern during the peri-pubertal period would increase life-time productivity across genetic types of Bos taurus cows. The objective was to determine the stayability, calf production, and weight of calf weaned across six calf crops. Heifers (n = 685) were placed on one of two developmental programs at 256 ± 1 d of age. Control heifers received a diet that provided 228 kcal ME·(body weight [BW], …

The Usda-Ars Ag100pest Initiative: High-Quality Genome Assemblies For Agricultural Pest Arthropod Research, Anna K. Childers, Scott M. Geib, Sheina B. Sim, Monica F. Poelchau, Brad S. Coates, Tyler J. Simmonds, Erin D. Scully, Timothy P. L. Smith, Christopher P. Childers, Renee L. Corpuz, Kevin Hackett, Brian Scheffler

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The phylum Arthropoda includes species crucial for ecosystem stability, soil health, crop production, and others that present obstacles to crop and animal agriculture. The United States Department of Agriculture’s Agricultural Research Service initiated the Ag100Pest Initiative to generate reference genome assemblies of arthropods that are (or may become) pests to agricultural production and global food security. We describe the project goals, process, status, and future. The first three years of the project were focused on species selection, specimen collection, and the construction of lab and bioinformatics pipelines for the efficient production of assemblies at scale. Contig-level assemblies of 47 species …

Enhancement Of Dry-Aged Beef Quality By Dietary Supplementation Of High Levels Of Vitamin E, David Manuel Velazco Marroquin

Department of Animal Science: Dissertations, Theses, and Student Research

Crossbred cattle (n = 150; 10/pen) were grain-finished with the dietary addition of 2,200 international units of vitamin E (α-tocopherol) per head per day for 100 days. One low-Choice carcass (n = 12) was selected, and strip loins were collected. Low Choice control carcasses (n = 12) were randomly selected from commercial, fed-cattle production to serve as controls. Strip loins were randomly assigned to wet or dry aging for 42 days. On day 42, control dry-aged and control wet-aged loins had the highest TBARS values and vitamin E wet and dry-aged loins had lower TBARS values ( …

Reference Transcriptomes Of Porcine Peripheral Immune Cells Created Through Bulk And Single-Cell Rna Sequencing, Juber Herrera-Uribe, Jayne E. Wiarda, Sathesh K. Sivasankaran, Lance Daharsh, Haibo Liu, Kristen A. Byrne, Timothy P. L. Smith, Joan K. Lunney, Crystal L. Loving, Christopher Tuggle

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Pigs are a valuable human biomedical model and an important protein source supporting global food security. The transcriptomes of peripheral blood immune cells in pigs were defined at the bulk cell-type and single cell levels. First, eight cell types were isolated in bulk from peripheral blood mononuclear cells (PBMCs) by cell sorting, representing Myeloid, NK cells and specific populations of T and B-cells. Transcriptomes for each bulk population of cells were generated by RNA-seq with 10,974 expressed genes detected. Pairwise comparisons between cell types revealed specific expression, while enrichment analysis identified 1,885 to 3,591 significantly enriched genes across all 8 …

Engineering And Characterization Of Human Β-Defensin-3 And Its Analogues And Microcin J25 Peptides Against Mannheimia Haemolytica And Bovine Neutrophils, Harpreet Dhingra, Kamaljit Kaur, Baljit Singh

Pharmacy Faculty Articles and Research

Mannheimia haemolytica-induced bovine respiratory disease causes loss of millions of dollars to Canadian cattle industry. Current antimicrobials are proving to be ineffective and leave residues in meat. Antimicrobial peptides (AMPs) may be effective against M. haemolytica while minimizing the risk of drug residues. Cationic AMPs can kill bacteria through interactions with the anionic bacterial membrane. Human β-Defensin 3 (HBD3) and microcin J25 (MccJ25) are AMPs with potent activity against many Gram-negative bacteria. We tested the microbicidal activity of wild-type HBD3, three HBD3 peptide analogues (28 amino acid, 20AA, and 10AA) derived from the sequence of natural HBD3, and MccJ25 …

Effects Of Farrowing Stall Layout And Number Of Heat Lamps On Sow And Piglet Behavior, Suzanne Leonard, Hongwei Xin, Tami M. Brown-Bandl, Brett C. Ramirez, Anna K. Johnson, Somak Dutta, G.A. Rohrer

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Farrowing stalls are used in the United States swine industry to reduce pre-weaning piglet mortality, enable efficient individual animal management, and decrease facility construction and operating costs. The quantity and quality of space provided for sows and piglets in farrowing stalls are important economic and welfare considerations. To further explore the impacts of farrowing stall space allocation, a large-scale field study was conducted to compare sow and piglet behavior when housed in three farrowing stall layouts (TSL – traditional stall layout, ECSL – expanded creep area stall layout, ESCSL – expanded sow and creep area stall layout) with either one …

Cattle Producer Motivations And Willingness To Participate In Industry Animal Welfare Program Verification, Chase Decoite

Master's Theses

As consumers have become increasingly interested in where their food comes from and how it is grown, the cattle and beef industry is without exception. Many consumers are beginning to question traditional animal husbandry practices and on-farm animal welfare protocol. It is essential that the food system meets new demands and understands consumer views. In order to address these concerns, the industry has rallied around the Beef Quality Assurance (BQA) program to set the standard for cattle welfare and beef quality, with the explicit goal of improving consumer confidence. The goal of this research is to determine if cattle producers …

Invited Review: Mineral Nutrition Considerations For Extensive Sheep Production Systems, W. C. Stewart, J. D. Scasta, J. B. Taylor, T. W. Murphy, A. A.M. Julian

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Purpose: Our purpose is to discuss contemporary and pertinent considerations related to mineral-supplementation strategies for sheep grazing marginal rangelands in extensively managed production systems. Sources: Grey literature, peer-reviewed literature, and data (published and unpublished) from co-authors were used. Synthesis: Precision trace-mineral nutrition of sheep that are grazing extensive production systems requires a comprehensive understanding of the sheep and the grazing environment. Generally, extensive sheep production systems are found in remote geographical regions composed of marginal rangelands not suitable for cultivated crop farming or improved forages. Sheep production is subject to the accessibility and availability of grazable forage, which may vary …

Some (Im)Material Girls, Living In (Im)Material Worlds, With Seeds, Stars, And Shit, Matthew Weiderspon

Theses and Dissertations

This writing situates material and gestural vocabularies cultivated in my artwork in relation to my lived experience; primarily my rural upbringing in Colorado. Scattered floor dispersals, calling sounds, and bodily movements desire reconsiderations of hope in precarity through a disorientation of place, association, scale, and language.

Variants In The Mitochondrial Genome Sequence Of Rhyzopertha Dominica (Fabricius) (Coleoptera: Bostrycidae), Lindsey C. Perkin, Timothy P.L. Smith, Brenda Oppert

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The lesser grain borer, Rhyzopertha dominica, is a coleopteran pest of stored grains and is mainly controlled by phosphine fumigation, but the increase in phosphine-resistant populations threatens efficacy. Some phosphine-resistant insects have reduced respiration, and thus studying the mitochondrial genome may provide additional information regarding resistance. Genomic DNA from an inbred laboratory strain of R. dominica was extracted and sequenced with both short (Illumina) and long (Pacific Biosciences) read technologies for whole genome sequence assembly and annotation. Short read sequences were assembled and annotated by open software to identify mitochondrial sequences, and the assembled sequence was manually annotated and verified …

Strain And Host-Cell Dependent Role Of Type-1 Fimbriae In The Adherence Phenotype Of Super-Shed Escherichia Coli O157:H7, Robab Katani, Indira T. Kudva, Sreenidhi Srinivasan, Judith B. Stasko, Megan Schilling, Lingling Li, Rebecca Cote, Chitrita Debroy, Terrance M. Arthur, Evgeni V. Sokurenko, Vivek Kapur

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Super-shed (SS) Escherichia coli O157 (E. coli O157) demonstrate a strong, aggregative, locus of enterocyte effacement (LEE)-independent adherence phenotype on bovine recto-anal junction squamous epithelial (RSE) cells, and harbor polymorphisms in non-LEE-adherence-related loci, including in the type 1 fimbriae operon. To elucidate the role of type 1 fimbriae in strain- and host-specific adherence, we evaluated the entire Fim operon (FimB-H) and its adhesion (FimH) deletion mutants in four E. coli O157 strains, SS17, SS52, SS77 and EDL933, and evaluated the adherence phenotype in bovine RSE and human HEp-2 adherence assays. Consistent with the prevailing dogma that fimH expression is genetically …

Effects Of Retail Case Environment And Led Lighting Temperature On Color Of Ground Beef Patties, Mesa Kutz

Animal Science Undergraduate Honors Theses

The objective of this study was to explore different type of retail cases and case environments, primarily lighting, to quantify the impact on shelf life. Coarse ground 80:20 beef from a local retail establishment was acquired between 7 and 10 d from the box date and fine ground through a 9.5 mm grinder plate, and then formed into 150.25 g patties with an automatic patty forming machine. Each patty was then individually packaged in PVC-overwrap packages and randomly assigned to one of the four different treatments: open, multideck cases with 3000 K lighting (OPEN3000),open 3500 K lighting (OPEN3500),enclosed, …

Exploring How Maternal Phosphorus Status Affects Calf Growth And Performance, Elizabeth Lafferty

Animal Science Undergraduate Honors Theses

This study will focus on how maternal phosphorus status of beef heifers affects the growth and performance of their calves. Heifers have been offered free-choice mineral with either 0 or 4% supplemental phosphorus from 30 days after weaning until calving. A study by H. Hilfiker, a University of Arkansas honors student, investigated the effects of these treatments from 30 days after weaning until 60 days after the breeding season when heifers were confirmed to be bred or open. For this developing heifer project 64 crossbred Angus heifers were assigned randomly into 8 groups (8 heifers/ group) before assigning each group …

Industry Funding Scheme Regulations: A Review Of Operation And Effectiveness, May 2021, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity published reports

Section 147 of the Biosecurity and Agriculture Management Act 2007 requires the Minister for Agriculture and Food to carry out a review of the operation and effectiveness of any regulations made for the purposes of establishing an Industry Funding Scheme (IFS) every five years.

In 2010, regulations establishing three IFSs commenced to address pest and disease threats relevant to Western Australia’s broadacre and pastoral cropping and livestock sectors. This report documents the second fifth-year anniversary statutory review of the following regulations:

• Biosecurity and Agriculture Management Industry Funding Scheme (Cattle) Regulations 2010

• Biosecurity and Agriculture Management Industry Funding Scheme …

Nebraska Cow–Calf Operations: Use Of Pricing Tools And Market Diversification Strategies To Manage Market Risk, Logan Kalkowski

Department of Agricultural Economics: Dissertations, Theses, and Student Research

This thesis is divided into three chapters. The first chapter of this thesis investigates years of research and data collection from multiple agencies to find connections to reasoning for producers to choose marketing and diversification tools used in their operation.

The second chapter examines cow-calf marketing and risk management practices in Nebraska. Marketing and risk management behavior are examined by using the University of Nebraska-Lincoln Cow-calf survey data collected in 2016. The survey captures characteristics of operations and their use of marketing and risk management practices. While it is important to understand what operations are doing currently, it is also …

Relationship Of Underline Scores And Production Traits In Beefmaster Cattle, Bryan R. Kutz

Graduate Theses and Dissertations

The objective of this study was to establish the relationship of underline scores (UDLS), production traits, and conformation scores in Beefmaster cattle born between 1976 through 2008. Records provided by Beefmaster Breeders United (BBU) were analyzed. Certified classifiers approved by the BBU recorded scores. Sizes and shapes of the underlines differentiated the scores (1-4). A score of one was used to designate the least amount of navel flap or sheath and a score of four represents the maximum amount of naval flap or sheath accepted for registration in the BBU. In this study the relationship of the traits was determined …

Methods To Reducing Weaning Stress In Early Weaned Spring Beef Calves, Matthew Daniel Hahn

Graduate Theses and Dissertations

Reducing weaning stress in beef calves can have dramatic benefits to the health, growth, and performance to beef calves post-weaning. Studies have shown that providing creep feed to calves during the pre-weaning period can improve their nutritional status, which can better prepare them for the stresses they will be exposed to during the weaning process. Studies have also shown that two-stage weaning can reduce the stresses associated with weaning. Previous research has suggested that this method resulted in dramatic changes in behavioral signs associated with stress. The objective of this study was to evaluate the effects of two-stage weaning with …

Sustainable Winter-Feeding Practices For Meat Goats Within The Southeastern United States, Nikisha Ryan, Nar Gurung, Jeremy Byrant, Frank Abrahamsen, Wendell Mcelhenney

Professional Agricultural Workers Journal

Winter feeding systems were evaluated on male Kiko meat goats against the traditional practice of raising goats in the winter months. Forty-Five Kiko bucks were randomly assigned to one of three treatments; Treatment 1, Treatment 2, and Treatment 3 consisted, respectively, of Cosaque Oats, annual Ryegrass, Crimson Clover mix, and 1.0% of BW concentrate supplementation with free choice hay. Forage clippings were analyzed for dry matter, crude protein, and neutral detergent fiber. Body weights were monitored over the grazing period. Blood samples were collected during the experiment, and animals were slaughtered for the evaluation of carcass traits. Data were analyzed …

Relationships Among Intramammary Health, Udder And Teat Characteristics, And Productivity Of Extensively Managed Ewes, Ryan M. Knuth, Whitney C. Stewart, Joshua B. Taylor, Bledar Bisha, Carl J. Yeoman, Megan L. Van Emon, Thomas W. Murphy

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Mastitis is an economically important disease and its subclinical state is difficult to diagnose, which makes mitigation more challenging. The objectives of this study were to screen clinically healthy ewes in order to 1) identify cultivable microbial species in milk, 2) evaluate somatic cell count (SCC) thresholds associated with intramammary infection, and 3) estimate relationships between udder and teat morphometric traits, SCC, and ewe productivity. Milk was collected from two flocks in early (<5 >d) and peak (30 to 45 d) lactation to quantify SCC (n = 530) and numerate cultivable microbial species by culture-based isolation followed by matrix-assisted laser …

Locus Of Heat Resistance (Lhr) In Meat-Borne Escherichia Coli: Screening And Genetic Characterization, Manita Guragain, Dayna M. Brichta-Harhay, James L. Bono, Joseph M. Bosilevac

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Microbial resistance to processing treatments poses a food safety concern, as treatment tolerant pathogens can emerge. Occasional foodborne outbreaks caused by pathogenic Escherichia coli have led to human and economic losses. Therefore, this study screened for the extreme heat resistance (XHR) phenotype as well as one known genetic marker, the locus of heat resistance (LHR), in 4,123 E. coli isolates from diverse meat animals at different processing stages. The prevalences of XHR and LHR among the meat-borne E. coli were found to be 10.3% and 11.4%, respectively, with 19% agreement between the two. Finished meat products showed the highest LHR …

Characterization Of The Domestic Goat Γδ T Cell Receptor Gene Loci And Gene Usage, Alexandria Gillespie, Al Yirsaw, Karthick P. Gunasekaran, Timothy P. L. Smith, Derek M. Bickhart, Michael Turley, Timothy Connelley, Janice C. Telfer, Cynthia L. Baldwin

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Goats and cattle diverged 30 million years ago but retain similarities in immune system genes. Here, the caprine T cell receptor (TCR) gene loci and transcription of its genes were examined and compared to cattle. We annotated the TCR loci using an improved genome assembly (ARS1) of a highly homozygous San Clemente goat. This assembly has already proven useful for describing other immune system genes including antibody and leucocyte receptors. Both the TCRγ (TRG) and TCRδ (TRD) loci were similarly organized in goats as in cattle and the gene sequences were highly conserved. However, the number of genes varied slightly …

Evaluation Of Condensed Algal Residue Solubles As An Ingredient In Cattle Finishing Diets And Its Effects On Digestibility And Fatty Acid Flow And A Comparison Of Single And Dual Implant Strategies In Finishing Heifers, John Calvin Gibbons

Department of Animal Science: Dissertations, Theses, and Student Research

Two experiments, a performance study (Exp. 1) and digestion study (Exp. 2) were conducted to evaluate a novel liquid feed, condensed algal residue solubles (CARS), in feedlot diets. In Exp. 1 and Exp. 2, steers were fed three inclusions of CARS at 0%, 2.5%, 5%. There were quadratic responses for greater carcass adjusted final BW, ADG, G:F, HCW, 12^th rib fat, and yield grade. There was a linear increase in marbling score and a linear decrease in DMI and LM area as CARS increased. In Exp. 2, CARS did not affect intake or digestibility. Fatty acid flow at the …

Cytokine And Haptoglobin Profiles From Shipping Through Sickness And Recovery In Metaphylaxis- Or Un-Treated Cattle, Carol G. Chitko-Mckown, Gary L. Bennett, Larry A. Kuehn, Keith D. Dedonder, Michael D. Apley, Gregory P. Harhay, Michael L. Clawson, Aspen M. Workman, Bradley J. White, Robert L. Larson, Sarah F. Capik, Brian V. Lubbers

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Fifty-six head of cattle, 28 animals with bovine respiratory disease complex (BRDC), and 28 healthy animals that were matched by treatment, sale barn of origin, day, and interactions among these variables, were identified from a population of 180 animals (60 each purchased at three sale barns located in Missouri, Tennessee, and Kentucky) enrolled in a study comparing animals receiving metaphylaxis to saline-treated controls. Cattle were transported to a feedlot in KS and assigned to treatment group. Blood samples were collected at Day 0 (at sale barn), Day 1, Day 9, and Day 28 (at KS feedlot), and transported to the …

Young Cattle Producers Conference Develops Leaders In Idaho, Carmen J. Willmore, Meranda Small, Sarah Baker, Jim Church

Journal of Extension

The need for young leadership in the beef industry has become a rising concern as the average age of primary operators increases. Extension educators developed a leadership conference for beef producers with the primary objective to educate and develop leadership qualities in young cattle producers. To achieve the objective participants, age 18-40 years old, had opportunities to learn all aspects of the industry through presentations, hands-on learning and industry tours. A post-conference survey revealed that participants planned to utilize what they learned and had a better understanding of the beef industry at the conclusion of the program.

Breed Differences In Placental Development During Late Gestation Between Chinese Meishan And White Crossbred Gilts In Response To Intrauterine Crowding, Jeremy R. Miles, Jeffrey L. Vallet

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The objective of this study was to evaluate placental development during late gestation (day 100) between Chinese Meishan (CM; n = 7) and White crossbred (WC; n = 5) gilts following intrauterine crowding induced by unilaterally hysterectomy-ovariectomy. Gross placental morphology and areolae density as well as histological morphology (i.e., folded bilayer and placental stroma) were analyzed using computer-assisted morphometry for placentas of the smallest and largest fetuses within each litter. There was a breed by fetal size interaction (P < 0.01) for areolae density in which placentas for large CM fetuses had greater areolae density compared to small CM fetuses, but the density of areolae was greater for CM fetuses compared to WC fetuses, irrespective of fetal size. The width of the folded bilayer was greater (P < 0.01) in placentas for WC gilts compared to CM gilts, irrespective of fetal size. Placentas for small fetuses had greater (P < 0.01) folded bilayer width compared to large fetuses, irrespective of breed. The placental stromal width was greater (P < 0.01) in placentas for large fetuses compared to small, irrespective of breed. The difference between stromal width in placentas between divergent-sized littermates, however, was greater (P = 0.05) in WC gilts compared to CM gilts, indicating there was a limited response to intrauterine crowding in CM gilts. These results indicate there is an altered placental development during late gestation in CM compared to WC gilts, thus, there are likely different mechanisms for responding to intrauterine crowding between breeds.

A Reference Genome Assembly Of Simmental Cattle, Bos Taurus Taurus, Michael P. Heaton, Timothy P.L. Smith, Derek M. Bickhart, Brian L. Vander Ley, Larry A. Kuehn, Jonas Oppenheimer, Wade R. Shafer, Fred T. Schuetze, Brad Stroud, Jennifer C. Mcclure, Jennifer P. Barfield, Harvey D. Blackburn, Theodore S. Kalbfleisch, Kimberly M. Davenport, Kristen L. Kuhn, Richard E. Green, Beth Shapiro, Benjamin D. Rosen

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Genomics research has relied principally on the establishment and curation of a reference genome for the species. However, it is increasingly recognized that a single reference genome cannot fully describe the extent of genetic variation within many widely distributed species. Pangenome representations are based on high-quality genome assemblies of multiple individuals and intended to represent the broadest possible diversity within a species. A Bovine Pangenome Consortium (BPC) has recently been established to begin assembling genomes from more than 600 recognized breeds of cattle, together with other related species to provide information on ancestral alleles and haplotypes. Previously reported de novo …

A Reference Genome Assembly Of American Bison, Bison Bison Bison, Jonas Oppenheimer, Benjamin D. Rosen, Michael P. Heaton, Brian L. Vander Ley, Wade R. Shafer, Fred T. Schuetze, Brad Stroud, Larry A. Kuehn, Jennifer C. Mcclure, Jennifer P. Barfield, Harvey D. Blackburn, Theodore S. Kalbfleisch, Derek M. Bickhart, Kimberly M. Davenport, Kristen L. Kuhn, Richard E. Green, Beth Shapiro, Timothy P.L. Smith

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Bison are an icon of the American West and an ecologically, commercially, and culturally important species. Despite numbering in the hundreds of thousands today, conservation concerns remain for the species, including the impact on genetic diversity of a severe bottleneck around the turn of the 20th century and genetic introgression from domestic cattle. Genetic diversity and admixture are best evaluated at genome-wide scale, for which a high-quality reference is necessary. Here, we use trio binning of long reads from a bison-Simmental cattle (Bos taurus taurus) male F1 hybrid to sequence and assemble the genome of the American plains bison (Bison …

Managing And Buying Bulls, Department Of Primary Industries And Regional Development, Western Australia

Animal production and livestock factsheets

Weaning percentage and therefore conception rate, can significantly influence profitability in the breeder herd.

Bulls need to be managed to optimise their performance for the mating period to maximise conception rates. For producers retaining females in the breeding herd, bulls can have a significant impact on future herd productivity through the genetics they pass on.

Various tools are available to assess how a bull will perform before purchase, including visual appraisal, obtaining a bull breeding soundness examination (BBSE) and utilising estimated breeding values (EBVs).