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2023

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Articles 61 - 90 of 1258

Full-Text Articles in Animal Sciences

Addressing Water Hyacinth (Pontederia Crassipes) Impacts On Aquatic Biota In Lake Okeechobee, Joseph Salerno Dec 2023

Addressing Water Hyacinth (Pontederia Crassipes) Impacts On Aquatic Biota In Lake Okeechobee, Joseph Salerno

All HCAS Student Capstones, Theses, and Dissertations

The incursion of water hyacinth, Pontederia crassipes in Lake Okeechobee has resulted in management systems to be implemented to reduce the coverage of the invasive macrophyte. Its residence in the Lake Okeechobee ecosystem and the effects it has on organisms in the lake, whether it be positive or harmful is unknown. This study attempted to assess the potential effects that water hyacinth has on aquatic biota in Lake Okeechobee. Biotic data were collected on open water, water hyacinth covered, and native vegetation covered habitats via hook-and-line fishing, electrofishing, baited minnow traps, and the sampling of plant roots over a thirteen-month …


Oliver Agee Catfish Neurocranium, Charlotte R. Oliver, Kristina Agee, David Kerstetter Dec 2023

Oliver Agee Catfish Neurocranium, Charlotte R. Oliver, Kristina Agee, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from 10.7 cm total length, [Catfish], collected by [Kerstetter].


Paternal Programming: The Role Of Seminal Plasma In Pregnancy Hemodynamics And Offspring Growth, Rebecca Michele Swanson Dec 2023

Paternal Programming: The Role Of Seminal Plasma In Pregnancy Hemodynamics And Offspring Growth, Rebecca Michele Swanson

Theses and Dissertations

Seminal plasma is commonly known to serve as a transport medium for sperm as it moves through the female reproductive tract for fertilization, however, more recent evidence demonstrates seminal plasma induces an expansive inflammatory response in the uterus. Murine models have found this inflammatory response is important for clearing pathogens and poor-quality sperm, eliciting the secretion of cytokines, chemokines, and embryokines that aid in embryo attachment and growth, placental angiogenesis, and blunting maternal immunity to the embryo. However, there is minimal research on the impacts of seminal plasma uterine priming in bovine, and more specifically embryo growth, uterine blood flow, …


Effects Of Supplementing Sources Of Zinc On Digestibility Parameters Of Beef Steers, Arminda Spikes James Dec 2023

Effects Of Supplementing Sources Of Zinc On Digestibility Parameters Of Beef Steers, Arminda Spikes James

Theses and Dissertations

Zinc (Zn) has numerous functions and life sustaining processes depend on the presence of Zn within the body. Zinc sulfate is a common form of Zn supplemented in the beef industry. Inorganic sulfate based minerals have been associated with negative rumen effects, mainly, decreased rumen fermentation and protozoa numbers. Our studies concluded no difference in digestibility of three different forms of Zn (zinc glycinate, zinc sulfate, and zinc hydroxychloride). Although overall digestibility did not change, there were specific mineral concentration differences after steers consumed concentrate treatment for 10 days. These differences implied that the mineral fed was consumed and absorbed …


Increased Coding Potential Of Bovine Herpesvirus 1, Victoria Jefferson Dec 2023

Increased Coding Potential Of Bovine Herpesvirus 1, Victoria Jefferson

Theses and Dissertations

Bovine respiratory disease (BRD) costs the cattle industry millions of dollars in costs in treatment and loss every year in the United States. A significant pathogen often contributes to BRD is Bovine Herpesvirus 1 (BoHV-1), a double stranded DNA virus with the ability to establish latency in the trigeminal ganglia and neurons. Primary infection with BoHV-1 results in immunosuppression that increases the risk of secondary bacterial infection and pneumonia. Because herpesviruses infect their hosts for life and can be reactivated in times of stress, BoHV-1 can present a recurring risk of BRD. The following research aims to expand the knowledge …


Evaluating Post-Release Survival And Habitat Utilization Of Undersized Swordfish (Xiphias Gladius) Caught By The U.S Pelagic Longline Fishery In The Western North Atlantic, Zachery Young Dec 2023

Evaluating Post-Release Survival And Habitat Utilization Of Undersized Swordfish (Xiphias Gladius) Caught By The U.S Pelagic Longline Fishery In The Western North Atlantic, Zachery Young

All HCAS Student Capstones, Theses, and Dissertations

This study used survivorship pop-up archival tags (sPAT) tags to determine the post-release survival and habitat use from twenty-four undersized swordfish captured in the northwest Atlantic Ocean during normal operation within the commercial U.S pelagic longline industry. From these deployments, sixteen tags were used to determine post-release survival, and eight of the surviving tags were used to analyze short-term habitat utilization. Mortality was estimated to be 43.8% and was not significantly affected by variables such as fish size, latitude caught, or horizontal distance traveled. The relatively high mortality, in combination with the high at-haulback mortality reported in previous studies, does …


Isolation Of The Toxic Principle Of Senecio Latifolius By Means Of The Sensory Receptors Of Sheep, Leendert D. Snyman Dec 2023

Isolation Of The Toxic Principle Of Senecio Latifolius By Means Of The Sensory Receptors Of Sheep, Leendert D. Snyman

Poisonous Plant Research (PPR)

The aversive substance of Senecio latifolius was isolated by means of the sensory receptors of sheep averted to S. latifolius. Chemical fractions refused due to the presence of the aversive substance sensed by the sheep were fractionated until a purified substance had been isolated. Nuclear magnetic resonance (NMR) analysis of the purified substance showed it to be sceleratine nitrogen oxide, the toxic principle of S. latifolius.


Common Snook (Centropomus Undecimalis) Neurocranium, Ryan Robison, Lesley Wallace, David Kerstetter Dec 2023

Common Snook (Centropomus Undecimalis) Neurocranium, Ryan Robison, Lesley Wallace, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium length of 16.5 cm Common Snook (Centropomus undecimalis), collected from Dr. Kerstetter's Lab on 10/23 by Ryan Robison and Lesley Wallace.


Yellow Grouper (Epinephelus Awoara), Tyler Jurain, David Kerstetter Dec 2023

Yellow Grouper (Epinephelus Awoara), Tyler Jurain, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.


Biophysical Factors Affecting Habitat Suitability For Crassostrea Virginica, Jason D. Tilley Dec 2023

Biophysical Factors Affecting Habitat Suitability For Crassostrea Virginica, Jason D. Tilley

Dissertations

Oyster reefs provide a variety of important ecosystem services. However, the mortality rate of eastern oyster, Crassostrea virginica, the dominant species that produces oyster reefs in the northern Gulf of Mexico, is increasing at an alarming rate due to a variety of abiotic and biological factors. I examined how biophysical factors, including the less-studied fatty acid profiles of the suspended particulate matter on which oysters feed, influenced morphometric condition of C. virginica.

I sampled suspended particulate matter (SPM) and oysters in-situ in the western Mississippi Sound, which historically supported the majority of oyster production in Mississippi waters. Sampling …


Jack Crevalle Neurocranium, Rodolfo A. Heredia, David Kerstetter Dec 2023

Jack Crevalle Neurocranium, Rodolfo A. Heredia, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.


Brown Bullhead Catfish (Ameiurus Nebulosus) Neurocranium, Reginald Leon Kempson Iii, David Kerstetter Dec 2023

Brown Bullhead Catfish (Ameiurus Nebulosus) Neurocranium, Reginald Leon Kempson Iii, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

Neurocranium from [6.3] cm total length (30 cm) [Brown Bullhead Catfish (Ameiurus nebulosus)], collected from [Dr.Kerstetters lab] on [6/December/2023] by [Reginald Kempson III]


Gag Grouper (Mycteroperca Microlepis) Neurocranium, Sierra Potts, David Kerstetter Dec 2023

Gag Grouper (Mycteroperca Microlepis) Neurocranium, Sierra Potts, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

10 cm neurocranium from Gag Grouper, Mycteroperca microlepis, collected from Snapper Mike's on 05/11/2023 by Sierra Potts


Oregon State Rank Assessment For Bonneville Redside Shiner (Richardsonius Balteatus Hydrophlox), Misty Nelson Dec 2023

Oregon State Rank Assessment For Bonneville Redside Shiner (Richardsonius Balteatus Hydrophlox), Misty Nelson

Institute for Natural Resources Publications

Oregon state conservation status assessment for Bonnevile redside shiner (Richardsonius balteatus hydrophlox) using NatureServe methodology, 2023.


Resolution Of The Tetrabothrius Jagerskioeldi Cryptic Species Complex Among Holarctic Alcidae (Charadriiformes): Cestodes Among Fraterculinae—Exploring Marine Diversity, Host Range, And Dynamic Oceanography In The Greater North Pacific, Eric P. Hoberg, Kaylen Marie Soudachanh, Svetlana K. Bondarenko Dec 2023

Resolution Of The Tetrabothrius Jagerskioeldi Cryptic Species Complex Among Holarctic Alcidae (Charadriiformes): Cestodes Among Fraterculinae—Exploring Marine Diversity, Host Range, And Dynamic Oceanography In The Greater North Pacific, Eric P. Hoberg, Kaylen Marie Soudachanh, Svetlana K. Bondarenko

MANTER: Journal of Parasite Biodiversity

In the biosphere, limits for diversity among species, communities, and biomes are revealed through intensive and extensive field-based inventory and assembly of voucher specimens and associated informatics examined in a phylogenetic, historical, ecological, and biogeographic arena. Archival resources for specimens and information contribute to a cumulative view of faunal structure and assembly under a comparative umbrella. Ultimately, species definitions, and inclusive partitions among populations and lineages, are fundamental in articulating hypotheses that examine interactions about evolution, the nature of organisms, and the condition of environments across space and time. We conclude our proposals establishing species limits for tapeworms of the …


Oregon State Rank Assessment For Klamath Smallscale Sucker (Catostomus Rimiculus), Misty Nelson Dec 2023

Oregon State Rank Assessment For Klamath Smallscale Sucker (Catostomus Rimiculus), Misty Nelson

Institute for Natural Resources Publications

Oregon state conservation status assessment for Klamath smallscale sucker (Catostomus rimiculus) using NatureServe methodology, 2023.


Oregon State Rank Assessment For Jenny Creek Sucker (Catostomus Rimiculus Pop. 1), Misty Nelson Dec 2023

Oregon State Rank Assessment For Jenny Creek Sucker (Catostomus Rimiculus Pop. 1), Misty Nelson

Institute for Natural Resources Publications

Oregon state conservation status assessment for Jenny Creek sucker (Catostomus rimiculus pop. 1) using NatureServe methodology, 2023.


Two New Phreatic Snails (Mollusca, Caenogastropoda, Cochliopidae) From The Edwards And Edwards-Trinity Aquifers, Texas, Kathryn E. Perez, Yamileth Guerrero, Roel Castañeda, Peter H. Diaz, Randy Gibson, Benjamin F. Schwartz, Benjamin T. Hutchins Dec 2023

Two New Phreatic Snails (Mollusca, Caenogastropoda, Cochliopidae) From The Edwards And Edwards-Trinity Aquifers, Texas, Kathryn E. Perez, Yamileth Guerrero, Roel Castañeda, Peter H. Diaz, Randy Gibson, Benjamin F. Schwartz, Benjamin T. Hutchins

School of Integrative Biological & Chemical Sciences Faculty Publications

The Edwards and Edwards-Trinity Aquifers of Texas have diverse stygofauna, including fifteen species of snails found in phreatic and hyporheic habitats. These species have the hallmarks of adaptation to subterranean environments including extremely small body size and the loss of pigmentation and eyes. Here we use an integrative taxonomic approach, using shell, radula, and anatomical features as well as mitochondrial and nuclear DNA data, to circumscribe a new genus and two new cavesnail species from Central Texas. Vitropyrgus lillianae gen. et sp. nov. is described from Comal Springs (Comal County) and Fessenden Springs (Kerr County) and distinguished by a glassy, …


Two New Phreatic Snails (Mollusca, Caenogastropoda, Cochliopidae) From The Edwards And Edwards-Trinity Aquifers, Texas, Kathryn E. Perez, Yamileth Guerrero, Roel Castañeda, Peter H. Diaz, Randy Gibson, Benjamin F. Schwartz, Benjamin T. Hutchins Dec 2023

Two New Phreatic Snails (Mollusca, Caenogastropoda, Cochliopidae) From The Edwards And Edwards-Trinity Aquifers, Texas, Kathryn E. Perez, Yamileth Guerrero, Roel Castañeda, Peter H. Diaz, Randy Gibson, Benjamin F. Schwartz, Benjamin T. Hutchins

School of Integrative Biological & Chemical Sciences Faculty Publications

The Edwards and Edwards-Trinity Aquifers of Texas have diverse stygofauna, including fifteen species of snails found in phreatic and hyporheic habitats. These species have the hallmarks of adaptation to subterranean environments including extremely small body size and the loss of pigmentation and eyes. Here we use an integrative taxonomic approach, using shell, radula, and anatomical features as well as mitochondrial and nuclear DNA data, to circumscribe a new genus and two new cavesnail species from Central Texas. Vitropyrgus lillianae gen. et sp. nov. is described from Comal Springs (Comal County) and Fessenden Springs (Kerr County) and distinguished by a glassy, …


Activity Patterns Of The Endangered Amargosa Vole (Microtus Californicus Scripensis), Austin N. Roy, Anna D. Rivera Roy, Deana L. Clifford, Janet E. Foley Dec 2023

Activity Patterns Of The Endangered Amargosa Vole (Microtus Californicus Scripensis), Austin N. Roy, Anna D. Rivera Roy, Deana L. Clifford, Janet E. Foley

School of Earth, Environmental, & Marine Sciences Faculty Publications

Examining the activity patterns of wildlife is an important aspect of understanding the ecology of a species and may be especially important for species of conservation concern. We used remotely triggered cameras to describe the daily and seasonal activity patterns and examine ecological factors that influence the activity of the Amargosa Vole (Microtus californicus scirpensis), a California endemic listed federally and by the state as Endangered, and is a marsh habitatspecialist in the Mojave Desert. We found that vole activity was greatest during crepuscular periods, followed by nocturnal and diurnal periods. We saw strong seasonal effects, with the highest activity …


State Of The Fisheries: Status Reports And Aquatic Resources Of Western Australia 2022/23, S.J. Newman, K.G. Santoro, D.J. Gaughan Dec 2023

State Of The Fisheries: Status Reports And Aquatic Resources Of Western Australia 2022/23, S.J. Newman, K.G. Santoro, D.J. Gaughan

Status Reports of the Fisheries and Aquatic Resources

Aquatic resources within Western Australia (WA) are in good condition, and this has positioned WA as a global leader in sustainable fisheries management. The sustainable fisheries of WA continue to support our strong economy and regional communities. Nonetheless, the lack of a consistent approach to build in the knowledge of Traditional Owners remains a gap in our longer term fisheries science in Western Australia.

Climate change and climate variability continues to impact fish stocks, challenging our ability to effectively monitor, assess, and manage fish stocks. We are continually working with our stakeholders, and the broader community to be adaptive, responsive, …


Liquid Chromatography Mass Spectrometry Method Development For The Determination Of Β–Defensins In Bovine Milk, Elisha Ogbebor Dec 2023

Liquid Chromatography Mass Spectrometry Method Development For The Determination Of Β–Defensins In Bovine Milk, Elisha Ogbebor

Masters Theses & Specialist Projects

Bovine mastitis is caused by a wide range of pathogens which results in a substantial economic loss for the dairy cattle livestock industry. β-defensins are a part of the innate immune system and act as the first line of defense against mastitis in bovine. β-defensins (~6- 10kDa) are antimicrobial peptides that contain about 28 to 67 amino acids, the presence of six cysteine residues results in their three disulfide bonds and a β-sheet structure. A few β- defensins have been identified in cows based on the site of occurrence and time of expression in tissue, but not in milk. A …


Towards The Quantification Of Estrone And 17Β-Estradiol Conjugates In Dairy Cattle Urine Sorptive Stir Bar Extraction And Gas Chromatography-Mass Spectometry, S.M. Istiak Ahmed Dec 2023

Towards The Quantification Of Estrone And 17Β-Estradiol Conjugates In Dairy Cattle Urine Sorptive Stir Bar Extraction And Gas Chromatography-Mass Spectometry, S.M. Istiak Ahmed

Masters Theses & Specialist Projects

The global concern regarding the presence of compounds with estrogenic properties in the environment has grown significantly. Endocrine-disrupting compounds (EDCs) are environmental pollutants that disrupt the endocrine systems of both wildlife and humans. Estrogens, with their potent estrogenic effects, are particularly noteworthy among the various substances with endocrine-disrupting properties. They are of concern due to their prevalence in the environment, especially due to dairy waste contamination. These lipophilic compounds can accumulate in livestock fat tissues, potentially entering the human food chain. Sulfated forms of estrone and β- estriol, which exhibit a higher degree of saturation than their non-conjugated counterparts, have …


Nutritional Evaluation Of Hydroponic Corn Fodder And Its Effect As Substitute For Feed Concentrates For Buffalo Calves, Charity I. Castillo, Phoebe Lyndia T. Llantada, Cyril P. Baltazar, Mary Rose D. Uy-De Guia, Reynald D. Amido, Arnel N. Del Barrio Dec 2023

Nutritional Evaluation Of Hydroponic Corn Fodder And Its Effect As Substitute For Feed Concentrates For Buffalo Calves, Charity I. Castillo, Phoebe Lyndia T. Llantada, Cyril P. Baltazar, Mary Rose D. Uy-De Guia, Reynald D. Amido, Arnel N. Del Barrio

The Philippine Agricultural Scientist

This study investigated the effects of hydroponic corn fodder on the growth performance of buffalo calves. Twelve Bulgarian Murrah buffalo calves were divided into two treatment groups: T1, fed with Napier grass and starter feed concentrates, and T2, fed with Napier grass, starter feed concentrates, and hydroponic corn fodder, replacing 50% of the starter feed concentrate. The study found no significant differences between the two groups' average total weight gain and average daily gain. However, the average daily feed intake was significantly higher in T2, possibly due to the tender and young nature of the hydroponic corn fodder. Dry matter …


The Influence Of Rusty Crayfish And Zebra Mussel Invasion On Yellow Perch Population Dynamics In North-Central Minnesota Lakes, Kendra Fink Dec 2023

The Influence Of Rusty Crayfish And Zebra Mussel Invasion On Yellow Perch Population Dynamics In North-Central Minnesota Lakes, Kendra Fink

Biology Graduate Theses

Over the last 50 years, Minnesota Department of Natural Resources standard fisheries survey catch rates of Yellow Perch have declined. Yellow Perch Perca flavescens are an important, widespread game fish, are essential prey species for Walleye Sander vitreus and other piscivorous fish and rely greatly on benthic resources through many life stages. Aquatic invasive species (AIS) like rusty crayfish Faxonius rusticus and zebra mussels Dreissena polymorpha have the potential to change water quality, vegetation and prey assemblages in aquatic systems and could cause shifts in energy flow, subsequently altering growth, condition, and maturity of native fish species. In this study, …


A Comparison Of Devices For Race Day Characterization Of North American Turfgrass Thoroughbred Racing Surfaces, Peter Schmitt, Wayne T. Sanderson, John Trey Rogers Iii, Tyler J. Barzee, Michael L. Peterson Dec 2023

A Comparison Of Devices For Race Day Characterization Of North American Turfgrass Thoroughbred Racing Surfaces, Peter Schmitt, Wayne T. Sanderson, John Trey Rogers Iii, Tyler J. Barzee, Michael L. Peterson

UK CARES Faculty Publications

Both pre-race meet and daily turf surface condition measurements are required by regula- tions adopted as part of the Horseracing Integrity and Safety Act (HISA). The Orono Biomechanical Surface Tester (OBST) is the primary device used for characterizing a racing surface and is used for the pre-meet inspections. Tools that are better suited for the daily testing of turf surfaces are also needed to meet the new federal regulations. The purpose of this study was to compare five simple tools commonly used in turf applications to the OBST. Data were collected with each of the six devices at plots chosen …


Yellow-Throated Warbler In Scotts Bluff County, Nebraska, Mark A. Brogie, Bill F. Huser, Jerry Probst Dec 2023

Yellow-Throated Warbler In Scotts Bluff County, Nebraska, Mark A. Brogie, Bill F. Huser, Jerry Probst

Nebraska Bird Review

Yellow-throated Warbler (Setophaga dominica) is a rare to uncommon migrant in eastern and central Nebraska. It is possibly a regular breeder locally at sites containing sycamores in several southeastern Nebraska counties bordering the Missouri River. There are few Nebraska reports after July, with more reports November–January than August–October. This species has three previous documented Nebraska Panhandle records, all from May.

1. One photographed 26 May 2009, at Crescent Lake NWR, Garden Co.

2. One seen and “well described” 06 May 1931, at Antioch, Sheridan Co.

3. A specimen taken 20 May 1917, at Oshkosh, Garden Co., was once …


Parrotfish Skull, Grace Mikus, David Kerstetter Dec 2023

Parrotfish Skull, Grace Mikus, David Kerstetter

All Scans: Kerstetter Fisheries and Avian Ecology 3D Scan Series

No abstract provided.


Rapid Evolution Of A(H5n1) Influenza Viruses After Intercontinental Spread To North America, Ahmed Kandeil, Christopher Patton, Jeremy C. Jones, Trushar Jeevan, Walter N. Harrington, Sanja Trifkovic, Jon P. Seiler, Thomas Fabrizio, Karlie Woodard, Jasmine C. Turner, Jeri Carol Crumpton, Lance Miller, Adam Rubrum, Jennifer Debeauchamp, Charles J. Russell, Elena A. Govorkova, Peter Vogel, Mia Kim-Torchetti, Yohannes Berhane, David Stallknecht, Rebecca Poulson, Lisa Kercher, Richard J. Webby Dec 2023

Rapid Evolution Of A(H5n1) Influenza Viruses After Intercontinental Spread To North America, Ahmed Kandeil, Christopher Patton, Jeremy C. Jones, Trushar Jeevan, Walter N. Harrington, Sanja Trifkovic, Jon P. Seiler, Thomas Fabrizio, Karlie Woodard, Jasmine C. Turner, Jeri Carol Crumpton, Lance Miller, Adam Rubrum, Jennifer Debeauchamp, Charles J. Russell, Elena A. Govorkova, Peter Vogel, Mia Kim-Torchetti, Yohannes Berhane, David Stallknecht, Rebecca Poulson, Lisa Kercher, Richard J. Webby

United States Department of Agriculture Wildlife Services: Staff Publications

Highly pathogenic avian influenza A(H5N1) viruses of clade 2.3.4.4b underwent an explosive geographic expansion in 2021 among wild birds and domestic poultry across Asia, Europe, and Africa. By the end of 2021, 2.3.4.4b viruses were detected in North America, signifying further intercontinental spread. Here we show that the western movement of clade 2.3.4.4b was quickly followed by reassortment with viruses circulating in wild birds in North America, resulting in the acquisition of different combinations of ribonucleoprotein genes. These reassortant A(H5N1) viruses are genotypically and phenotypically diverse, with many causing severe disease with dramatic neurologic involvement in mammals. The proclivity of …


Effects Of Dietary Rumen-Protected Choline Supplementation To Periparturient Dairy Cattle On Inflammation, Metabolism, And Performance During An Intramammary Lipopolysaccharide Challenge, Turner H. Swartz, Barry Bradford, Laman Mamedova, Kari Estes Dec 2023

Effects Of Dietary Rumen-Protected Choline Supplementation To Periparturient Dairy Cattle On Inflammation, Metabolism, And Performance During An Intramammary Lipopolysaccharide Challenge, Turner H. Swartz, Barry Bradford, Laman Mamedova, Kari Estes

Dairy Science and Food Science Faculty Publications: 2022 to Present

Recent studies have suggested that dietary rumen-protected choline (RPC) supplementation can modulate immune function, attenuate inflammation, and improve performance in periparturient dairy cattle; however, this has yet to be evaluated during a mastitis challenge. Therefore, the objective of this study was to examine the effects of supplementation and dose of RPC on metabolism, inflammation, and performance during an intramammary lipopolysaccharide (LPS) challenge. Parous Holstein cows (parity, mean ± SD, 1.9 ± 1.1 at enrollment) were blocked by calving month and randomly assigned within block to receive either 45 g/d of RPC (20.4 g/d of choline ions; CHOL45, n = 18), …