Open Access. Powered by Scholars. Published by Universities.®

Animal Sciences Commons™

Open Access. Powered by Scholars. Published by Universities.®

2017

Discipline
Institution
Keyword
Publication
Publication Type
File Type

Articles 271 - 300 of 1104

Full-Text Articles in Animal Sciences

Kentucky Warbler (Vol. 93, No. 2), Kentucky Library Research Collections May 2017

Kentucky Warbler (Vol. 93, No. 2), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.


Species Differences In Egocentric Navigation: The Effect Of Burrowing Ecology On A Spatial Cognitive Trait In Mice, Jason N. Bruck, Noah A. Allen, Kelsey E. Brass, Brian A. Horn, Polly Campbell May 2017

Species Differences In Egocentric Navigation: The Effect Of Burrowing Ecology On A Spatial Cognitive Trait In Mice, Jason N. Bruck, Noah A. Allen, Kelsey E. Brass, Brian A. Horn, Polly Campbell

Faculty Publications

© 2017 The Association for the Study of Animal Behaviour Efficient navigation is a critical component of fitness for most animals. While most species use a combination of allocentric (external) and egocentric (internal) cues to navigate through their environment, subterranean environments present a unique challenge in that visually mediated allocentric cues are unavailable. The relationship between egocentric spatial cognition and species differences in ecology is surprisingly understudied. We used a maze-learning task to test for differences in egocentric navigation between two closely related species of mice, the eastern house mouse, Mus musculus musculus, and the mound-building mouse, Mus spicilegus. The …


Demographic Performance Of Brownlip Abalone : Exploration Of Wild And Cultured Harvest Potential, Lachlan W.S. Strain, Alexander Hesp, Frank Fabris, Anthony M. Hart May 2017

Demographic Performance Of Brownlip Abalone : Exploration Of Wild And Cultured Harvest Potential, Lachlan W.S. Strain, Alexander Hesp, Frank Fabris, Anthony M. Hart

Fisheries Research Reports

This research provides the most comprehensive evaluation of Brownlip abalone (Haliotis conicopora) biology and fisheries assessment to date, thereby laying a solid foundation for future management of the Brownlip abalone fishery in Western Australia (WA). For wild populations, it has provided the most reliable estimates of natural and fishing mortality, size composition and the first to model growth throughout all stages of life.


Beyond Capture: Development And Validation Of A Method To Assess Body Condition In Mule Deer (Odocoileus Hemionus) Using Camera Traps, Rachel A. Smiley 6820420 May 2017

Beyond Capture: Development And Validation Of A Method To Assess Body Condition In Mule Deer (Odocoileus Hemionus) Using Camera Traps, Rachel A. Smiley 6820420

University Scholar Projects

Advances in technology and availability associated with camera traps have resulted in a rapid rise in their use to monitor wildlife distribution, abundance, and behavior. We focus on assessing body condition, a new application of camera traps. Body condition indices must relate to the percent body fat if they are to be useful. To acquire measurements of body fat, most body condition indices require capture or mortality of animals to estimate, which has limitations when applied to free-ranging animals. We developed a non-invasive, visual body condition index (VBCI) to assess body condition of mule deer that can be applied …


Time-Activity And Energy Budgets Of Eared Grebes (Podiceps Nigricollis) At Salton Sea, Samantha San Francisco May 2017

Time-Activity And Energy Budgets Of Eared Grebes (Podiceps Nigricollis) At Salton Sea, Samantha San Francisco

Theses

Time-activity budgets have been a widely accepted way of approximating the daily energy expenditure of many species, including waterbirds. For this study, focal animal sampling was used to study Eared Grebes (Podiceps nigricollis) at their post-wintering stopover site, Salton Sea. Daylight time-activity budgets were converted to energy budgets using energy equivalents for diving, preening, swimming, and resting on water developed for Eared Grebes previously by H. I. Ellis (1994). Post-wintering Eared Grebes arrive at Salton Sea in waves at differing times in the spring. As a result, there can be individuals in a variety of physiological states during …


Evaluating Habitat-Based Niche Requirements And Potential Recruitment Bottlenecks For Imperiled Bluehead Sucker (Catostomus Discobolus), Bryan C. Maloney May 2017

Evaluating Habitat-Based Niche Requirements And Potential Recruitment Bottlenecks For Imperiled Bluehead Sucker (Catostomus Discobolus), Bryan C. Maloney

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Changes to rivers that alter physical and thermal habitat may cause fish population abundance to decline, due to fewer individuals maturing and entering the adult population. The Weber River has become highly degraded with many dams and diversions altering fish habitat, river volume, velocity, and temperature, and limiting movement between reaches. Bluehead suckers (Catostomus discobolus) occupy only 47% of their historical range and the genetically-distinct Weber River (northern UT) population is declining and contains few young, juvenile fish. My objectives were to determine whether spawning and rearing habitat available in the Weber River may be limiting bluehead sucker …


Genetic Polymorphisms Of The Glucocorticoid Receptor And Interleukin-8 Receptor Genes Are Related To Production Traits And Hair Coat Score In Crossbred Cattle, Avery Deaton May 2017

Genetic Polymorphisms Of The Glucocorticoid Receptor And Interleukin-8 Receptor Genes Are Related To Production Traits And Hair Coat Score In Crossbred Cattle, Avery Deaton

Animal Science Undergraduate Honors Theses

The objective of this thesis was to identify polymorphisms in the glucocorticoid receptor (GR) and interleukin-8 receptor (CXCR2) genes and to associate genotypes between the above mentioned polymorphisms and production traits in crossbred cattle. The hypothesis was that polymorphisms will exist for GR and CXCR2 genes and will be linked to production traits. Glucocorticoid receptors have been positively associated with higher milk yields, lactose content, feed intake, and feed conversion rates. Interleukin-8 genes are part of the innate immune response and help with many aspects of female reproduction health, such as protecting the embryo from the maternal immune system during …


The Bovine Rumen Microbiome Revealed By Different Fractions Of Rumen Contents, Ashlee L. Breakstone May 2017

The Bovine Rumen Microbiome Revealed By Different Fractions Of Rumen Contents, Ashlee L. Breakstone

Animal Science Undergraduate Honors Theses

The bovine rumen microbiota is very important in terms of animal functionality and digestion. The fermentative capability of the rumen provides means for the digestion of complex plant material that is indigestible by humans. The rumen is the working ground for millions of microorganisms, primarily bacteria, to digest and ferment feed into volatile fatty acids and bacterial protein for the animal’s energy and protein needs, respectively. Of significant importance is the rumen microbiomes ability to conform to certain factors such as genetics, feed, or geographic location. These changes can have a direct measure on animal growth, health, and performance. The …


Predicting The Onset Of Parturition By Determining Calcium In Prepartum Milk Of Sheep, Lensey Watson May 2017

Predicting The Onset Of Parturition By Determining Calcium In Prepartum Milk Of Sheep, Lensey Watson

Animal Science Undergraduate Honors Theses

Multiple methods of pregnancy detection can be used in sheep production. The gestation period for sheep is usually between 144 and 150 days; however, depending on the breed and age of the ewes, this number can vary. A breeding marker, such as a breeding crayon, is used to detect when the ewe is bred by the ram, and the date the ewe will lamb is typically calculated based on an average 145-day gestation. Unfortunately, this method provides only a rough estimate of when the ewe could lamb. In addition, because sheep routinely give birth to multiples (twins, triplets, and, sometimes, …


Beyond Capture: Development And Validation Of A Method To Assess Body Condition In Mule Deer (Odocoileus Hemionus) Using Camera Traps, Rachel A. Smiley 6820420 May 2017

Beyond Capture: Development And Validation Of A Method To Assess Body Condition In Mule Deer (Odocoileus Hemionus) Using Camera Traps, Rachel A. Smiley 6820420

Honors Scholar Theses

Advances in technology and availability associated with camera traps have resulted in a rapid rise in their use to monitor wildlife distribution, abundance, and behavior. We focus on assessing body condition, a new application of camera traps. Body condition indices must relate to the percent body fat if they are to be useful. To acquire measurements of body fat, most body condition indices require capture or mortality of animals to estimate, which has limitations when applied to free-ranging animals. We developed a non-invasive, visual body condition index (VBCI) to assess body condition of mule deer that can be applied …


Identification Of Molecular Signatures Of Environmental And Nutritional Stress Responses In Poultry, Phuong Hieu Nguyen May 2017

Identification Of Molecular Signatures Of Environmental And Nutritional Stress Responses In Poultry, Phuong Hieu Nguyen

Graduate Theses and Dissertations

Poultry production plays an important role in the food supply and security for billions of people. Poultry meat is considered the most efficient, nutritious source of protein, without religious taboos, and is relatively inexpensive. However, the poultry industry is facing substantial challenges. The global environmental temperature has experienced dramatic changes in the last few years. Modern broilers are not well adapted to environmental challenges (heat and cold stress), which have resulted in heavy economic loss in the poultry industry worldwide. In addition, the use of feed restriction regimens in management of breeders as well as the transportation of young chicks …


Phenology, Habitat Use, And The Impacts Of Wetland Management On Autumn Migrating Rails In Missouri, Auriel M.V. Fournier May 2017

Phenology, Habitat Use, And The Impacts Of Wetland Management On Autumn Migrating Rails In Missouri, Auriel M.V. Fournier

Graduate Theses and Dissertations

Rails (Family: Rallidae) are among the least studied birds in North America, in large part due to their elusive nature. As a wetland-dependent species, understanding the timing of their migration and their habitat needs during migration is especially important since management needs to be timed to balance the needs of many species. I developed and verified a new distance sampling based nocturnal ATV spotlight survey because traditional call-broadcast surveys are not effective during autumn migration because of the drop off in call rate after the breeding season. These surveys allow us to ask point-level questions about what habitats rails select …


Influence Of Exogenous Phytase Level And Interaction With Supplmental Xylanase On Live Performance And Phosphorus Retention Of Broilers, Peyton Gilbert May 2017

Influence Of Exogenous Phytase Level And Interaction With Supplmental Xylanase On Live Performance And Phosphorus Retention Of Broilers, Peyton Gilbert

Graduate Theses and Dissertations

Two 3-week experiments were conducted, each using 480 day-old male broilers placed randomly among 96 battery cages (5 per cage; 680cm2 per bird), to evaluate the influence of phytase supplementation, with or without supplemental xylanase, on bird performance. Individual body weights and cage feed weights were taken on days 0, 7, 14, and 21, and mean BW, body weight gain (BWG), feed intake (FI), and FCR over each period calculated. Mortality was recorded daily and used to calculate livability and adjust FCR in both trials. In trial 2, excreta was collected by cage from 14 to 21 days for bone …


Heterogeneity Of Avian Breeding Habitat On Grazing Lands Of The Northern Great Plains, Maggi S. Sliwinski May 2017

Heterogeneity Of Avian Breeding Habitat On Grazing Lands Of The Northern Great Plains, Maggi S. Sliwinski

School of Natural Resources: Dissertations, Theses, and Student Research

Native rangelands in the Great Plains are largely privately owned and used for beef production. Vegetation heterogeneity is important for maintaining biodiversity, but private land may be more homogenous than desired. My research had two components: 1) to examine whether a variety of grazing strategies created vegetation heterogeneity in a large, intact rangeland, and 2) to understand beef producers’ attitudes about vegetation heterogeneity.

First, I sampled vegetation structure, composition, and bird abundance at multiple plots on eleven management units in Cherry County, Nebraska. Units were managed with commonly used grazing strategies (e.g., short-duration grazing and season-long continuous grazing). I examined …


Capture Theory & State Regulation Of Animal Cruelty, Kyla Henderson May 2017

Capture Theory & State Regulation Of Animal Cruelty, Kyla Henderson

Political Science Theses and Capstones

In this thesis I theorize that regulatory capture impacts animal cruelty laws in several different ways. Capture theory is the process by which regulatory agencies eventually come to be dominated by the industries they were charged with regulating. Regulatory capture happens when a regulatory agency, formed to act in the public’s interest, eventually acts in ways that benefit the industry it is supposed to be regulating, rather than the public. Animal cruelty regulations are an important factor in the way that everyday products are produced. Capture theory affecting animal cruelty is an important look at the growing concern with animal …


Does Personality Similarity In Bottlenose Dolphin Pairs Influence Dyadic Bond Characteristics?, Kelsey R. Moreno May 2017

Does Personality Similarity In Bottlenose Dolphin Pairs Influence Dyadic Bond Characteristics?, Kelsey R. Moreno

Master's Theses

Social structures are critical to the success of many species and have repercussions on health, well-being, and adaptation, yet little is known about the factors which shape these structures aside from ecology and life history strategies. Dyadic bonds are the basis of all social structures; however, mechanisms for formations of specific bonds or patterns in which individuals form which types of bonds have yet to be demonstrated. There is a variety of evidence indicating personality may be a factor in shaping bonds, but this relationship has not been explored with respect to bond components and is yet to be demonstrated …


Do Pinnipeds Have Personality? Coding Harbor Seal (Phoca Vitulina) And California Sea Lion (Zalophus Californianus) Behavior Across Contexts, Amber J. De Vere May 2017

Do Pinnipeds Have Personality? Coding Harbor Seal (Phoca Vitulina) And California Sea Lion (Zalophus Californianus) Behavior Across Contexts, Amber J. De Vere

Master's Theses

Personality has now been studied in species as diverse as chimpanzees (King & Figueredo, 1997) and cuttlefish (Carere et al., 2015), but marine mammals remain vastly underrepresented in this area. A broad range of traits have been assessed only in the bottlenose dolphin (Highfilll & Kuczaj, 2007), while consistent individual differences in a few specific behaviors have been identified in grey seals (Robinson et al., 2015; Twiss & Franklin, 2010; Twiss, Culloch & Pomeroy, 2011; Twiss, Cairns, Culloch, Richards & Pomeroy, 2012). Furthermore, the context component of definitions of personality is not often assessed, despite evidence that animals may show …


Sparking A Dolphin's Curiosity: Individual Differences In Dolphins' Reactions To Surprising And Expectation-Violating Events, Malin Katarina Lilley May 2017

Sparking A Dolphin's Curiosity: Individual Differences In Dolphins' Reactions To Surprising And Expectation-Violating Events, Malin Katarina Lilley

Master's Theses

Non-scientific literature consistently describes dolphins as “curious animals,” but there has been little systematic research on curiosity in dolphins. Curiosity in humans and certain non-human animal species, including birds and non-human primates, has been studied by examining individual differences in exploration and reactions to novel stimuli. Additionally, research has explored how human infants and non-human animals react when an event violates their expectations. The present study explored dolphins’ reactions to spontaneously surprising and expectation-violating stimuli. The reactions of dolphins, 15 bottlenose (Tursiops truncatus) and 6 rough-toothed (Steno bredanensis), at Gulf World Marine Park were analyzed in …


Life History Of The Non-Native Invasive Red Lionfish (Pterois Volitans) In The Northern Gulf Of Mexico, Alexander Q. Fogg May 2017

Life History Of The Non-Native Invasive Red Lionfish (Pterois Volitans) In The Northern Gulf Of Mexico, Alexander Q. Fogg

Master's Theses

Invasive Red Lionfish (Pterois volitans) were first detected in the northern Gulf of Mexico (nGOM) in 2010 and since then their numbers have increased dramatically. From 2010 to 2015, more than 15,000 Red Lionfish were collected opportunistically from the nGOM for this study. Length and weight relationships differed significantly among ecoregions by sex and there was clear sexual dimorphism in size with males being larger and heavier. Red Lionfish age ranged from 0-4.5 years old and males achieved greater growth rate (K) and asymptotic maximum lengths (Linf) compared to females and these parameters were also different …


The Effect Of Boat Type On Bottlenose Dolphin (Tursiops Trucatus) Behavior In The Mississippi Sound, Maria Zapetis May 2017

The Effect Of Boat Type On Bottlenose Dolphin (Tursiops Trucatus) Behavior In The Mississippi Sound, Maria Zapetis

Master's Theses

Increases in oceanic shipping are a global phenomenon, and a leading cause of concern for marine animal welfare. While it may be difficult to assess the effect of boat traffic on all species in all contexts, it is vital to report anthropogenic impacts where longitudinal data is available, and doubly so where a dearth of information exists. The purpose of this study is to describe how dolphin behavior changed in the presence of boats in the Mississippi Sound between 2006 and 2012, and more specifically, to detail how different boat types impacted dolphins’ behavioral states. This study is unique in …


Laterality Preferences Completing A Tool-Use Task In Asian Small-Clawed Otters (Aonyx Cinereus), Stephanie E. James May 2017

Laterality Preferences Completing A Tool-Use Task In Asian Small-Clawed Otters (Aonyx Cinereus), Stephanie E. James

Honors Theses

Laterality is defined as the occurrence of each cerebral hemisphere having asymmetric control over the different sides of the body, leading to preferences to use one side of the body over the other for various behaviors. Many types of laterality exist, but handedness is the form that is most predominantly assessed. Handedness in animals is of special interest to laterality researchers, as humans were once thought to be the only species that exhibited lateralized hand preferences. The aim of the current study was to determine if Asian small-clawed otters exhibit significant paw preferences in behaviors associated with completing a tool-use …


Pragmatic Understanding Of Bottlenose Dolphins (Tursiops Truncatus) Use Of A Two-Way Communication System, Pepper Reid Hanna May 2017

Pragmatic Understanding Of Bottlenose Dolphins (Tursiops Truncatus) Use Of A Two-Way Communication System, Pepper Reid Hanna

Dissertations

Pragmatics focuses on how a communication system is used to achieve a communicative goal, the social context of the communication, and the organizational structure of communications (Horn & Ward, 2004; McLaughlin, 1998). There is evidence of pragmatics within animal communication systems. For example, context appears to be an important component in both signal production and a receiver’s response in vervet monkeys (Seyfarth et al. 1980).

MA Thesis: http://aquila.usm.edu/masters_theses/170/

The current study used an underwater keyboard to establish a two-way communication system between humans and dolphins. The purpose of this study was to determine if, under these conditions, dolphins displayed pragmatic …


Efficacy Of Cognitive Enrichment For Bottlenose Dolphins (Tursiops Truncatus): Evaluation Of Planning Abilities Through The Use Of A Novel Problem Solving Task, Lisa Kay Lauderdale May 2017

Efficacy Of Cognitive Enrichment For Bottlenose Dolphins (Tursiops Truncatus): Evaluation Of Planning Abilities Through The Use Of A Novel Problem Solving Task, Lisa Kay Lauderdale

Dissertations

Environmental enrichment is a key component to improving the psychological and physiological well being of animals in human care. Enrichment can be achieved through a variety of modalities, including the addition of objects and scents, or by providing the animals with additional challenges. The effectiveness of specific enrichment should be evaluated on a case-by-case basis to determine if the desired result is achieved. Environmental enrichment devices (EED’s) can be utilized to present novel problems to animals in human care. When confronted with a novel problem, dolphins can plan their behavior to create a more efficient strategy then previously modeled.

The …


Growth Patterns And Size-Group Interactions In Hatchery-Reared Age-0 Lake Sturgeon, Joseph M. Susco May 2017

Growth Patterns And Size-Group Interactions In Hatchery-Reared Age-0 Lake Sturgeon, Joseph M. Susco

All NMU Master's Theses

Raising lake sturgeon (Acipenser fulvescens) in hatcheries is an important tool for rehabilitation and conservation, but is expensive. When hatchery sturgeon do not grow large enough before stocking, their odds of survival decline significantly and resources are wasted. We investigated the potential limiting effect of large lake sturgeon on the growth rate of smaller conspecifics housed in the same tank, and the possibility of increasing growth rates by separating individuals into different size classes. We also examined plasma cortisol levels of the different size classes over time as an indicator of stress levels, and levels of free plasma …


Late Quaternary Dragon Lizards (Agamidae: Squamata) From Western Australia, Julie Rej May 2017

Late Quaternary Dragon Lizards (Agamidae: Squamata) From Western Australia, Julie Rej

Electronic Theses and Dissertations

Fossil Agamidae from Western Australia have been the subject of limited study. To aid in fossil agamid identification, Hocknull (2002) examined the maxilla and dentary of several extant species from Australia and determined diagnostic characters for various species groups. In the study here, fossil agamids from two localities in Western Australia, Hastings Cave and Horseshoe Cave, were examined, grouped, and identified to the lowest unambiguous taxonomic level. Morphometric analyses were conducted to compare morphotypes, and find additional diagnostic characters. From Hastings Cave there were two maxilla morphotypes and three dentary morphotypes. Based on identifications, taxa present at this locality were …


Evaluation Of Absorbezz On Swine Reproductive Health And A Comparative Study Of Two Commercially Available Swine Show Feeds, Scott G. Sutton May 2017

Evaluation Of Absorbezz On Swine Reproductive Health And A Comparative Study Of Two Commercially Available Swine Show Feeds, Scott G. Sutton

Electronic Theses and Dissertations

The efficacy of a feed supplement fed to gilts and sows was tested in study I. The supplement was stated to improve reproductive soundness, conception rates, immune health, and overall well-being of livestock. Thirty gilts were split into a control group (Base diet) and a treatment group (Absorbezz® 0.32 mL on base diet). Upon completion of the trial results indicate the treatment group significantly underperformed (

Two commercially available show pig growing rations were tested in study II to find a high quality, cost efficient product. A total of 10 pigs (5 barrows, 5 gilts), at an average weight of …


Backgrounding Calf Management Strategies Using Corn Residue And Double Cropped Forages, Jordan L. Cox-O'Neill May 2017

Backgrounding Calf Management Strategies Using Corn Residue And Double Cropped Forages, Jordan L. Cox-O'Neill

Department of Animal Science: Dissertations, Theses, and Student Research

Approximately 70% of calves in the U.S. are born in the spring and weaned in the fall, this results in a large supply of calves potentially available for backgrounding over the winter. Backgrounding systems positively impact the beef industry by efficiently using forage resources available. These systems also provide production value by enhancing the calf’s frame size, mature BW (increase HCW), and health prior to entering the feedlot. In Nebraska opportunities to integrate both crop and livestock production, by backgrounding calves abound. Approximately 13,100 metric tons of corn residue or 34,000 Animal Unit Months are available for grazing in Nebraska. …


Estimates Of Corn Residue Quality, Tasha M. King May 2017

Estimates Of Corn Residue Quality, Tasha M. King

Department of Animal Science: Dissertations, Theses, and Student Research

Two experiments were conducted to determine the effect of corn residue harvest method on animal performance and diet digestibility. Corn residue harvest methods used in these experiments were low-stem, high-stem, and conventional. Steers had greater ADG when fed low-stem in a growing diet compared to high-stem and conventional residues. Addition of supplemental RUP to corn-residue based diets resulted in greater ADG and G:F in steers. Digestibility of DM, OM, and NDF were greatest in lambs fed diets containing low-stem residue. Low-stem residue had greater digestible energy (DE) compared to high-stem and conventional, which did not differ.

Lastly, a study was …


Evaluation Of Protein And Fiber From Distillers’ Grains Plus Solubles In Finishing Beef Cattle Diets, Zachary E. Carlson May 2017

Evaluation Of Protein And Fiber From Distillers’ Grains Plus Solubles In Finishing Beef Cattle Diets, Zachary E. Carlson

Department of Animal Science: Dissertations, Theses, and Student Research

Four studies were conducted to evaluate the components of distillers grains plus solubles in finishing beef cattle diets. Experiment 1 evaluated the effects of five composites of feedstuffs formulated to be similar in nutrient composition to DDGS on finishing performance of cattle. Experiment 2 evaluated isolating the protein from distillers grains using a feedstuff from the wet milling ethanol industry to determine the impacts of protein on the feeding value of WDGS in finishing performance of cattle. Experiments 3 and 4 evaluated the effects of protein from distillers grains with the diets used in Exp. 2 on site of digestion, …


Use Of Byproducts In Forage-Based, Post-Weaning Beef Systems And Effects Of Serial Slaughter On Performance And Profitability, Robert Gregory Bondurant Jr. May 2017

Use Of Byproducts In Forage-Based, Post-Weaning Beef Systems And Effects Of Serial Slaughter On Performance And Profitability, Robert Gregory Bondurant Jr.

Department of Animal Science: Dissertations, Theses, and Student Research

Crude glycerin was included in high-forage beef growing diets at 0, 4, 8, and 12% diet DM to determine the effect on fiber digestion by evaluating changes in microbial species abundance, NDF digestibility, and VFA concentrations. Total tract NDF digestibility decreased with increasing inclusion of GLY in high-forage diets. However, there was no decrease in in situ NDF digestibility and Fibrobacter succinogenes microbial populations were unaffected, indicating that fiber digestion was not directly affected by inclusion of GLY. Acetate decreased while propionate and butyrate VFA proportions increased as GLY increased.

Spayed heifers were utilized in a 2-yr study to evaluate …