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Articles 211 - 240 of 296
Full-Text Articles in Animal Sciences
The Effects Of Carbonated Marinade On The Shelf Life Of Enhanced Pork, Maria Ofelia Guerra
The Effects Of Carbonated Marinade On The Shelf Life Of Enhanced Pork, Maria Ofelia Guerra
LSU Master's Theses
Brine incorporation into meat products has become a well established practice in the United States. Brines are used to enhance the eating quality of pork by improving its tenderness and juiciness. Carbon dioxide and carbon monoxide have been used in modified atmosphere packaging as antimicrobial agents and color stabilizers, respectively, to increase the shelf life of retail meats. The objective of this experiment was to analyze the effects of incorporating carbon dioxide and carbon monoxide into brine solutions to subsequently inject into raw chilled pork. Pork loins were injected with brine solutions containing 2.27% phosphates, 3.79% salt and dissolved gas …
A Novel Yogurt Product With Lactobacillus Acidophilus, Senthil Ganesh
A Novel Yogurt Product With Lactobacillus Acidophilus, Senthil Ganesh
LSU Master's Theses
Health benefits of Lactobacillus acidophilus include providing immune support for infections or cancer, providing a healthy replacement of good bacteria in the intestinal tract following antibiotic therapy, reducing occurrence of diarrhea in humans, aiding in lowering cholesterol and improving the symptoms of lactose intolerance. Consumer demand exists for new dairy products. There are several types of yogurt like stir curd, set curd and drinkable yogurt and they all need to be refrigerated. Moreover there are very few dairy products that can be stored at room temperature and not many dairy foods are finger foods. A novel yogurt product like a …
Color And Tenderness In Chilled Or Frozen Pork Loin Chops After Antioxident Dipping And Modified Atmosphere Packaging, Dennis Michael Price
Color And Tenderness In Chilled Or Frozen Pork Loin Chops After Antioxident Dipping And Modified Atmosphere Packaging, Dennis Michael Price
LSU Master's Theses
The objective of this research was to evaluate color and tenderness in chilled or frozen pork loin chops after antioxidant dipping and modified atmosphere packaging. Loin chops were dipped in 0.3 M calcium chloride, 2.0 % sodium ascorbate, 0.2 M calcium ascorbate, or 0.3 M calcium ascorbate. Non-dipped chops served as controls. Chops were packaged in high oxygen (80% O2 / 20% CO2) or no oxygen (80% N2 / 20% CO2) and stored chilled (4° C) for 7 days or frozen (-18° C) for 21 days. After storage, chops were displayed under continuous fluorescent lighting for 3 or 6 days. …
The Effect Of A Condensed Tannin Containing Forage, Sericea Lespedeza On Existing And Challenge Infections Of Haemonchus Contortus In Sheep, Leigh Ann Chafton
The Effect Of A Condensed Tannin Containing Forage, Sericea Lespedeza On Existing And Challenge Infections Of Haemonchus Contortus In Sheep, Leigh Ann Chafton
LSU Master's Theses
Haemonchus contortus is one of the most threatening parasites to small ruminant production. Control has traditionally relied on the use of anthelmintics. Control has waned because the worm population has developed resistance to most of the currently available anthelmintics and alternative control measures are needed. Current consumer pressure is to reduce the use of chemicals in agricultural products, thus pushing control methods to more natural and acceptable approaches. One such approach is the feeding of condensed tannin containing forages either as fresh forage or dried products (hay, meal, pellets, etc). This study was conducted to determine the effect of a …
Cryopreservation And Intracytoplasmic Sperm Injection With Bovine Epididymal Spermatozoa, Carlos Andres Guerrero
Cryopreservation And Intracytoplasmic Sperm Injection With Bovine Epididymal Spermatozoa, Carlos Andres Guerrero
LSU Doctoral Dissertations
Recently, interest in the preservation of epididymal sperm as a potential source of valuable genes for genome resource banks has escalated. The development of a successful protocol to recover and cryopreserve sperm harvested from the epididymides would salvage germplasm from genetically valuable males that are injured and can no longer mate or have unexpectedly died and can be used as a model for the preservation of male gametes from endangered species. In a series of experiments, epididymal sperm was successfully harvested, cryopreserved and used for intracytoplasmic sperm injection. In Experiment I, ethylene glycol was found to cause significantly (P<0.05) less osmotic damage to bovine sperm during a one step addition and/or removal at 4°C as compared with glycerol in all concentrations evaluated. Furthermore, prolonged exposure (5 days at 4°C) of ethylene glycol was found to be less toxic than glycerol to sperm. In Experiment II, it was demonstrated that glycerol was more effective than ethylene glycol in providing protection against freezing injury during the cryopreservation process in the concentrations evaluated. In Experiment III, it was demonstrated that epididymal sperm retrieval using seminal plasma is beneficial to enhance sperm overall and progressive motility characteristics and to protect it from morphological abnormalities derived from the freezing process. In Experiment IV, a one step dilution process for removal of glycerol from cryopreserved epididymal sperm was found to significantly affect plasma membrane integrity and mitochondrial function of sperm previously exposed to seminal plasma. However, seminal plasma exposure did not have any significant detrimental effect on acrosome integrity. Furthermore, it was demonstrated that the longevity and survivability in vitro during a 4-hour incubation period at 37°C of post-thaw epididymal sperm exposed to seminal plasma prior to cryopreservation was not compromised when compared with the control extended sperm. In Experiment V, we have demonstrated that fertilization, blastocyst and fetal development could be achieved with cryopreserved bovine epididymal sperm by intracytoplasmic sperm injection (ICSI). To our knowledge, this is the first report in the United States and second in the world to use bovine epididymal sperm for ICSI. We achieved far markedly improved blastocyst rates over those results recently reported in the first study originating in Japan.
Comparing Bermudagrass And Bahiagrass Cultivars At Different Stages Of Harvest For Dry Matter Yield And Nutrient Content, Ryan Thomas Dore'
Comparing Bermudagrass And Bahiagrass Cultivars At Different Stages Of Harvest For Dry Matter Yield And Nutrient Content, Ryan Thomas Dore'
LSU Master's Theses
Rapid growth of warm-season grasses such as bermudagrass (Cynodon dactylon) and bahiagrass (Paspalum notatum) is associated with a decline in their nutritional value. This study was initiated to provide production and composition data with different cultivars of bermudagrass (common, Russell, Jiggs) and bahiagrass (Tifton-9, Pensacola, Argentina). Dry matter (DM), ash, crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF) composition and production were evaluated every two weeks for a ten-week period on six different cultivars. Also, Russell bermudagrass was evaluated in a second trial very similar to the first trial for composition and production but was started …
The Effects Of Micronutrients On Pullets And Broilers, Danielle Armantine Lauzon
The Effects Of Micronutrients On Pullets And Broilers, Danielle Armantine Lauzon
LSU Master's Theses
Two experiments were conducted to determine the availability of various vitamin E (E) sources (absorbed to verxite or adsorbed to silica) in broilers. In Experiment 1, at 100 and 300 IU supplemental E, an average of 94 and 44% of E intake from verxite and silica, respectively, was excreted in the feces, but at 30 IU E, 49 and 45% of E intake was excreted in the feces. In Experiment 2 at 30 IU, 52 and 43% of E intake from verxite and silica was excreted (source, P < 0.02). Based on these results, E adsorbed to silica is more available. Two experiments were conducted to determine the relative bioavailability of organic versus inorganic sources of either Mn or Zn. In Experiment 1, Mn as MnSO4 or a Mn amino acid complex (Availa-Mn) were compared. In Experiment 2, ZnSO4 or a Zn amino acid complex (Availa-Zn) were compared. The results indicate that Availa-Mn is a more available source of Mn than MnSO4, but Availa-Zn is not as available as a source of Zn as ZnSO4. An experiment was conducted to determine the effects of organic sources of Zn, Mn, and Cu on White Leghorn pullet performance. Treatment diets consisted of a control diet with 66 ppm Zn as ZnSO4 or a combination of ZnSO4 and Availa-Zn, 66ppm Mn as MnSO4 or a combination of MnSO4 and Availa-Mn, and 10 ppm Cu as CuSO4 or a combination of CuSO4 and a Cu amino acid complex (Availa-Cu). Diets are inorganic sources (IO), organic Zn (OZ), organic Zn and Mn (OZM), or organic Zn, Mn, and Cu (OZMC). Addition of OZM increased (P < 0.08) intestinal tensile strength and increased (P < 0.06) grams of ash per bone compared with OZMC. Pullets fed OZ had a higher (P < 0.08) bone concentration of all minerals than pullets fed OZM. Total tibia Mn was decreased (P < 0.06) by OZMC, but total tibia Cu was increased (P < 0.08) by OZ addition over IO or OZM.
Vitrification And Dehydration For The Preservation Of Gametes, Allison E. Moisan
Vitrification And Dehydration For The Preservation Of Gametes, Allison E. Moisan
LSU Doctoral Dissertations
Of the 36 species of felines in the world, all except the domestic cat are listed as endangered or threatened. To preserve the genetic diversity of felines and other species, genome resource banks have been established. Due to limited availability of germ cells for research, studies must use models to optimize the techniques before they are applied to endangered species. In this study, preservation of oocytes and spermatozoa was examined using the bovine as a model for felines. In the first series of experiments, bovine and feline oocytes were dehydrated, vitrified, warmed and cultured to assess their ability to undergo …
Evaluation Of A Plasmid Delivery System For Production Of Gnrh And Ghrh In The Horse And Goat, William Andrew Storer
Evaluation Of A Plasmid Delivery System For Production Of Gnrh And Ghrh In The Horse And Goat, William Andrew Storer
LSU Doctoral Dissertations
The efficacy of a novel plasmid delivery system was assessed for long-term expression of gonadotropin releasing hormone (GnRH) and growth hormone releasing hormone (GHRH) in horses and goats. The efficacy of the technology was demonstrated using 3 novel plasmids: pSEAP [expressing secreted embryonic alkaline phosphatase (SEAP)], pGHRH (expressing GHRH), and pGnRH (expressing GnRH). Geldings were electroporated with a reporter plasmid expressing SEAP in 3 muscle sites. Expression of SEAP, measured from jugular plasma samples, indicated muscle specificity for uptake and expression of the plasmid. Concentrations of SEAP were greatest (P < 0.05) after pectoralis injection, which was chosen as the site for electroporation in subsequent studies. In a second experiment, stallions were electroporated with pGHRH or pSEAP to evaluate the effect of long-term GHRH treatment on the growth hormone (GH) axis and testicular function. Stallions treated with pGHRH had increased (P < 0.05) plasma concentrations of IGF-I, increased (P < 0.05) volume of accessory sex gland fluid, and increased (P < 0.05) number of normal spermatozoa in the ejaculate relative to controls. In the third experiment, stallions were electroporated with pGnRH or pSEAP to test the effects of GnRH on the reproductive axis. Treatment with pGnRH increased (P < 0.05) plasma testosterone concentrations to d 56 and increased (P < 0.01) the LH response to GnRH on d 21, but did not alter (P > 0.1) seminal characteristics evaluated after 36 d of treatment. In …
Gamete And Cell Technologies For Use In Biological Resource Banking, Liesl Nel-Themaat
Gamete And Cell Technologies For Use In Biological Resource Banking, Liesl Nel-Themaat
LSU Doctoral Dissertations
Biological resource banking is becoming important for endangered species conservation. A series of experiments were conducted to address issues concerning collection and utilization of biomaterials from Gulf Coast Native (GCN) sheep (model species) (Ovis aries) and Eland antelope (Taurotragus oryx). In the first experiment, two ejaculates were collected 10 minutes apart from each of five rams three times a week for three weeks to maximize output and minimize handling time. Semen volume, concentration and total number of spermatozoa were significantly greater in first ejaculates, whereas pre-cooled, cooled and post-thaw motility, as well as sperm survival, were greater in second ejaculates. …
Reproductive Characteristics Of High Body Condition Mares With High Versus Low Leptin Concentrations, Cara A. Waller
Reproductive Characteristics Of High Body Condition Mares With High Versus Low Leptin Concentrations, Cara A. Waller
LSU Master's Theses
Two experiments were performed to discover what, if any, reproductive differences exist in high body condition (BCS) mares with varying concentrations of leptin. Previous research showed that mares with high body condition scores can have a range of leptin levels, from very low (<5 ng/mL) to very high (>10 ng/mL). Earlier results indicated that most mares with high body condition scores maintain estrous cycles or show significant follicular activity during the winter. Among these high BCS mares, about 30% of them exhibit hyperleptinemia and hyperinsulinemia. The first experiment was designed to compare the reproductive characteristics of high BCS mares with high versus low leptin …
Effect Of Chromium Propionate And Fat Source On Growth, Carcass Traits, And Meat Quality In Swine And The Effect Of Chromium Propionate On Growth And Carcass Traits In Broilers, Ashunti Ria Jackson
Effect Of Chromium Propionate And Fat Source On Growth, Carcass Traits, And Meat Quality In Swine And The Effect Of Chromium Propionate On Growth And Carcass Traits In Broilers, Ashunti Ria Jackson
LSU Master's Theses
Three experiments were conducted to determine the effects of the chromium propionate (CrProp) in swine and poultry diets. Two broiler experiments, each utilizing 1,460 Ross X Ross broilers, were conducted to evaluate the effects of 0, 200, 400, or 800 ppb CrProp on growth performance and carcass traits in 0 to 42 and 0 to 49 d-old broilers. In Exp. 1, 400 ppb Cr decreased (P < 0.10) ADG and G:F in the grower phase, but mortality was reduced (P < 0.10) in broilers fed 200 ppb or 800 ppb Cr. In the finisher phase, G:F was increased (P < 0.10) in broilers fed all dietary levels of Cr, and mortality was reduced (P < 0.10) in broilers fed 200 and 400 ppb Cr. Chromium did not affect the overall growth data or carcass traits. In Exp. 2, CrProp did not affect growth performance. The results indicated that Cr as CrProp improved G:F in the later phases of growth and reduced mortality in Exp. 1 but not in Exp. 2. In Exp. 3, 108 crossbred Yorkshire gilts were used to examine the effects of 0 or 200 ppb CrProp on iodine value, fatty acid profile of fat, growth, carcass traits, and pork quality from gilts fed tallow or choice white grease (CWG). Chromium decreased (P < 0.03) 10th rib back fat and increased (P < 0.02) percent muscle. Gain:feed was increased (P < 0.003) and ADFI decreased by fat addition. Fat increased hot carcass weight (P < 0.07) and dressing percent (P < 0.02). Average back fat, 9th rib CL, and total loss were decreased (P = 0.02 to 0.04) by tallow. Belly width was increased (P < 0.08) by tallow, but belly bending on both the teatline and scribe side were increased (P = 0.004 to 0.01) by CWG. Iodine values on belly and loin samples were increased (P < 0.001) by CWG. Overall, Cr affected BF and percent muscle and the inclusion of tallow decreased (P = 0.03 to 0.09) belly and loin iodine value further than CWG or no added fat.
The Effect Of Diets Varying In Dietary Cation-Anion Difference Fed In Late Gestation And In Lactation On Sow Productivity, Melanie Lynn Roux
The Effect Of Diets Varying In Dietary Cation-Anion Difference Fed In Late Gestation And In Lactation On Sow Productivity, Melanie Lynn Roux
LSU Master's Theses
Eighty-six primiparous or multiparous sows and their pigs were used to evaluate the effects of changing dietary cation-anion difference (DCAD) in late gestation and in lactation on sow productivity. Twenty sows were used in Exp. 1, and the dietary treatments consisted of a positive control (PC) corn-soybean meal (C-SBM) diet with four levels of DCAD (140, 99, 75, and 45 mEq/kg). These DCAD’s were achieved by four levels of SoyChlor (SC) additive (0, 1.5, 2.5, and 3.5%). In Exp. 2, 66 sows were used and the dietary treatments consisted of the diets providing DCAD of 140 and 45 mEq/kg. The …
Genetic (Co) Variance For Growth And Tenderness Related Traits In Purebred Brahman Steers, Joshua Dean Domingue
Genetic (Co) Variance For Growth And Tenderness Related Traits In Purebred Brahman Steers, Joshua Dean Domingue
LSU Master's Theses
Paternal half-sib groups of Brahman bull calves were purchased at weaning from purebred Brahman producers in Louisiana. Following backgrounding and grazing on ryegrass the steers shipped to a commercial feedlot in south Texas. Steers were slaughtered in groups when they reached an approximate average endpoint weight of 535 kg and 10mm backfat. After a 24 hr chill, carcasses were ribbed, data collected, and a longissimus muscle sample taken for calpastatin assay. Two 2.54 cm thick steaks were randomly removed from the large end of the strip loin, vacuum packaged, and randomly selected for 7 and 14 days of aging. Genetic …
Effects Of Protein Sources With Differing Ruminal Degradation Characteristics On Nutrient Digestibilities And Flows Through Various Segments Of The Gastrointestinal Tract Of Non-Lactating Holstein Cows, Taylor Whitney Braud
LSU Master's Theses
Six non-lactating Holstein cows with ruminal and duodenal cannulas were used in a replicated 3x3 Latin square design experiment to investigate the effects of supplemental protein source on ruminal and total tract nutrient digestibility. Supplemental protein was provided from soybean meal (SBM), expeller processed soybean meal (EXP), or menhaden fish meal (FM). Basal diets consisted of (DM basis) 33% corn silage, 20% bermudagrass hay, 27% ground corn, and 2% minerals and vitamins. Supplemental protein was provided as (DM % of total diet) 18% soybean meal (SOY), 17% soybean meal and 1% fish meal (FM), or 12% soybean meal and 6% …
The Effect Of Age And Sex On The Growth Patterns Of Bovine Cell Lines, Jeho Shin
The Effect Of Age And Sex On The Growth Patterns Of Bovine Cell Lines, Jeho Shin
LSU Master's Theses
The influence of donor animal sex or age on in vitro bovine cell culture was evaluated to provide foundation information for the selection of donor tissue for nuclear transfer. Skin biopsies were taken from each of sixteen individuals including four bulls (B), four cows (C), four male calves (MC), four female calves (FC). At passage 2, cells from in vitro culture of cell lines were influenced not by gender but by age in the mean cell generation time (MGT). When evaluating familial lineage, comparison between related and unrelated groups showed that most comparisons do not show significant differences in lag …
Comparing Calf Sex Ratio And Semen Sex Ratio Determined By Conventional Pcr, Tara Marie Taylor
Comparing Calf Sex Ratio And Semen Sex Ratio Determined By Conventional Pcr, Tara Marie Taylor
LSU Master's Theses
Thirty-nine ejaculates from 8 Holstein bulls were collected. Straws of semen (n=750) were distributed among dairies in three states. Ten straws per ejaculate were sent to Louisiana State University (LSU) Dairy Improvement Center for conventional polymerase chain reaction (PCR) analysis. Spermatozoal DNA was extracted and PCR analysis was done using one primer set amplifying a single copy 125 base pair (bp) section of the Bos taurus factor IX (Christmas factor) precursor (found on the X chromosome) and another primer set amplifying a single copy section of the Bos taurus sex determining region Y protein (SRY) gene (found on the Y …
The Effect Of Dietary Phosphorus Level And Phytase Supplementation On Growth Performance, Bone Breaking Strength, And Phosphorus Excretion In Broilers, Syrena Powell
LSU Master's Theses
This research was conducted to determine the effects of feeding different P levels with and without phytase supplementation on broiler growth performance, bone breaking strength (BBS), and P excretion. An experiment with 4 trials was conducted with 7,840 Ross x Ross straight run broilers. For each trial, 1,960 broilers were allotted on d 0 to 4 treatments with 7 replications per treatment with 70 broilers per replication. The broilers were fed a 4 period feeding program consisting of starter (0 to 14 d), grower (14 to 32 d), finisher (32 to 41 d), and withdrawal (41 to 50 d) periods. …
Characteristics Of Reduced Fat Milks As Influenced By The Incorporation Of Folic Acid, Kamalesh Achanta
Characteristics Of Reduced Fat Milks As Influenced By The Incorporation Of Folic Acid, Kamalesh Achanta
LSU Master's Theses
Folic acid plays an important role in the prevention of neural tube defects such as spina bifida and anencephaly, heart defects, facial clefts, urinary abnormalities and limb deficiencies. Milk and milk products serve as a potential source for folic acid fortification due to the presence of folate binding proteins which seem to be involved in the folate bioavailability. Though milk is a not a good source of folic acid, fortification could help in the prevention of the above mentioned defects. The objective of this study was to examine the physico-chemical characteristics of reduced fat milks fortified with folic acid. Reduced …
Reconstruction Of Nuclear Transfer Embryos In Goats And Cattle [Electronic Resource], Allison Morris Landry
Reconstruction Of Nuclear Transfer Embryos In Goats And Cattle [Electronic Resource], Allison Morris Landry
LSU Doctoral Dissertations
The low survival rates of nuclear transfer fetuses and neonates in goats and cattle have been linked to placental abnormalities. A series of studies was designed to investigate the possibility of supplementing nuclear transfer embryos with electrofused embryos to generate placental tissue in goats and cattle. The initial study was designed to determine if the breeding season of goats could be extended with hCG treatment. Progesterone concentrations in treated does increased but pregnancy rates were unaffected. In the second study, goat embryos were electrofused and combined with nuclear transfer embryos at the 8-cell stage to produce the first offspring as …
Regulation Of Glucose Metabolism In Dairy Cattle, Christie Cheatham Stanley
Regulation Of Glucose Metabolism In Dairy Cattle, Christie Cheatham Stanley
LSU Doctoral Dissertations
The experiments described herein are designed to elucidate many metabolic changes that occur to regulate the partitioning of nutrients between production and animal maintenance with particular regard to glucose metabolism and insulin sensitivity. The onset of milk production and parturition causes dramatic stress to the cow. The challenge of improving metabolism during this period is being addressed by nutritional management with the possibility of the addition of supplements. The use of Ca-propionate as a supplement did not affect glucose metabolism in transition cows. The data revealed that insulin sensitivity was low during the transition period, but the tissue responses to …
Amino Acid Requirements And Low Crude Protein, Amino Acid Supplemented Diets For Swine And Poultry, Dustin Wade Dean
Amino Acid Requirements And Low Crude Protein, Amino Acid Supplemented Diets For Swine And Poultry, Dustin Wade Dean
LSU Doctoral Dissertations
The purpose of this research was to determine requirements for amino acids that limit the use of crystalline amino acids, to determine the effects of reducing crude protein, and to evaluate the problems associated with low crude protein diets for swine and poultry. Four experiments were conducted to determine the Lys and sulfur amino acid requirements for 5- to 10-kg pigs. The results of these experiments suggest pigs in this weight range require a diet containing 1.40% true digestible (TD) Lys and the ratio of sulfur amino acids to Lys is not greater than 0.54. Two experiments were conducted to …
Effects Of Dietary Protein Level And Fish Meal On Growth And Hormonal Status Of Weaned Dairy Calves, Patricia Terrell Richardel
Effects Of Dietary Protein Level And Fish Meal On Growth And Hormonal Status Of Weaned Dairy Calves, Patricia Terrell Richardel
LSU Master's Theses
Eight weaned Holstein steers approximately 4 mo of age (mean BW = 119.47 ± 2.65 kg) were used in a replicated 4 x 4 Latin square designed experiment to study the effects of protein source and level on performance and anabolic hormonal status of weaned dairy calves. Treatments consisted of two diets containing either 16 or 20 % crude protein (CP) with or without fish meal (FM). Experimental diets were corn silage based with soybean meal (SBM) as the source of ruminal degradable protein and FM as the source of ruminal undegradable protein. The animals were fed their respective diets …
The Effect Of Holding Bovine Oocytes In Follicular Fluid On Subsequent Fertilization And Embryonic Development, Angela Marie Klumpp
The Effect Of Holding Bovine Oocytes In Follicular Fluid On Subsequent Fertilization And Embryonic Development, Angela Marie Klumpp
LSU Master's Theses
The objective of Experiment 1 was to determine the effect of bovine follicular fluid (bFF) on nuclear maturation. Treatment A (Control) oocytes were stained with Hoechst-33342 immediately after aspiration from follicles, whereas, oocytes in Treatment B were held in bFF for 12 hours at 38¢ªC and then stained to determine nuclear status. No significant difference was detected between treatment groups. Results indicate that bFF inhibits resumption of meiosis. The objective of Experiment 2 was to determine the effect of bFF on embryonic development. Oocytes in Treatment A (Control) were placed into in vitro maturation (IVM) for 22 hours followed by …
Cryopreservation Of Bovine And Caprine Oocytes By Vitrification, Sabrina Marie Luster
Cryopreservation Of Bovine And Caprine Oocytes By Vitrification, Sabrina Marie Luster
LSU Master's Theses
Cryopreservation of animal oocytes will permit germplasm of valuable or unique females to be preserved for extended times. The objective of this research was to derive a procedure to cryopreserve bovine oocytes by vitrification to be used as recipients for somatic cell nuclear transfer (SCNT). Caprine oocytes vitrified by the same procedure were assayed by cytological examination of microtubules. In the first two of three experiments, bovine oocytes matured in vitro were vitrified in a mixture of ethylene glycol (EG), dimethylsulfoxide (Me2SO) and trehalose, and then subjected to in vitro fertilization (IVF) or SCNT. For vitrification, oocytes were first exposed …
Bakso (Traditional Indonesian Meatball) Properties With Postmortem Condition And Frozen Storage, Dino Rahardiyan
Bakso (Traditional Indonesian Meatball) Properties With Postmortem Condition And Frozen Storage, Dino Rahardiyan
LSU Master's Theses
Bakso is a finely comminuted boiled Indonesian meat product that is traditionally made of starch, salt and emulsified prerigor or early postmortem meat and often sold from street vendors. Recently processors have begun to commercially manufacture bakso. This research was conducted to investigate the substitution of early postmortem meat with postrigor meat to allow more efficient manufacturing and raw material procurement. The first experiment was to determine the properties of bakso with three tapioca starch concentrations added to early or late postmortem beef. No differences (p<0.05) were observed in bakso properties of composition and texture, though bakso made of post-rigor meat had slightly less elasticity, strength and shear. These disadvantages were compensated by incorporating 15% starch concentration, indicating that the replacement of early postmortem meat with postrigor meat was applicable in industrial mass production of bakso. The second phase of experiments was to investigate the properties of bakso with different frozen storage times of raw postrigor meat and after different times of frozen storage for bakso made from postrigor and early postmortem meat. Postrigor meat substitution for early postmortem meat in bakso with 15% tapioca starch resulted in minimum composition and textural differences (p>0.05). Meat stored frozen for 2 and 4 months was still suitable as raw …
Mineral Utilization In Poultry As Affected By Virginiamycin Or Mineral Source, Tanika Ivel O'Connor-Dennie
Mineral Utilization In Poultry As Affected By Virginiamycin Or Mineral Source, Tanika Ivel O'Connor-Dennie
LSU Master's Theses
Four experiments were conducted to evaluate the effects of diets with reduced Ca or nonphytate P (nPP) levels with supplemental virginiamycin (Vm) on growth performance and bone response variables in chicks. All diets were corn-soybean meal based and all treatments were replicated six or eight times with five or six chicks each. Reducing dietary nPP from 0.45 to 0.15, 0.25, or 0.35% decreased most growth and bone response variables. The addition of Vm to diets containing 0.35% nPP and above increased both growth and bone response variables. The addition of Vm to diets containing 0.25% nPP and below had no …
Efficacy Of Prepartum Intramammary Lactating Cow Treatment In Dairy Heifers, Christopher B. Norman
Efficacy Of Prepartum Intramammary Lactating Cow Treatment In Dairy Heifers, Christopher B. Norman
LSU Master's Theses
Mastitis in prepartum dairy heifers has been recognized as a significant economic problem for the dairy industry. Intramammary infusion of cephapirin sodium was completed in 20 treatment animals and the results were compared to 25 non-treated control animals. Bacteriological data from the quarter milk samples were used to determine and compare initial infection rates and cure rates following calving between treatment and control groups. Comparisons were also made between groups for differences in reproductive performance, milk yield, somatic cell count and milk ketone concentration. Milk yield did not differ between groups for either DHIA monthly average kg/day, 305 day actual …
The Use Of Altrenogest To Control Reproductive Function In Beef Cattle, Clarence Edward Ferguson
The Use Of Altrenogest To Control Reproductive Function In Beef Cattle, Clarence Edward Ferguson
LSU Doctoral Dissertations
There has been great controversy involving progesterone (P4) levels during early pregnancy in cattle. The objectives of these experiments were to determine the effect of an early low dose administration of P4 or altrenogest (ALT) on pregnancy rates in repeat breeder (RPB) females, if an increase in pregnancy rates could result from a direct effect of P4 on the embryo and if ALT could support pregnancy in the absence of a functional CL. Firstly, ALT was evaluated for use as a progestin in cattle by synchronizing estrus in beef heifers. There were no differences in the …
Statistical Models And Genetic Evaluation Of Binomial Traits, Jose Lucio Guerra
Statistical Models And Genetic Evaluation Of Binomial Traits, Jose Lucio Guerra
LSU Doctoral Dissertations
Generalized mixed model methodology and MCMC simulations were used to estimate genetic parameters for calving rate and calf survival with the normal, probit, and logistic models. Calving rate and calf survival were defined as 0 each time a cow failed to calf or a calf failed to survive to weaning age, otherwise they were set to 1. Data were available on 1,458 cows and on 5,015 calves. Cows produced a total of 4,808 records over 4 discrete generations of rotational crosses between Angus, Brahman, Charolais, and Hereford from 1977 to 1995. The heritability of calving rate and calf survival, the …