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Articles 181 - 210 of 296
Full-Text Articles in Animal Sciences
Determinants Of Fluid Milk Quality, Jose Luis Vargas
Determinants Of Fluid Milk Quality, Jose Luis Vargas
LSU Master's Theses
The objectives of this study were to provide an overview of fluid milk quality in the U.S., determine if there is a correlation between rapid and traditional tests, determine if results from different analyses correlate with a sensory analysis and to determine any correlation between any of the analyses done in this study. Whole and 2% milks were received from 17 dairy processing plants located in the west, midwest, and southern regions of the U.S in duplicates. Samples were pasteurized at the processing plants by HTST method (161-175oF for 15-25 seconds). For standard plate counts at day 0 no significant …
Effect Of Milk Replacer Composition On Growth And Rumen Development Of Neonatal Holstein Calves, Anthony Joseph Bridges
Effect Of Milk Replacer Composition On Growth And Rumen Development Of Neonatal Holstein Calves, Anthony Joseph Bridges
LSU Master's Theses
A study was conducted to determine effects of differing fat and protein levels in commercial milk replacers on the growth performance and rumen development of neonatal Holstein calves. Fifty-three calves (23 male; 30 female) were assigned to one of three dietary treatments which included 3 milk replacers (20/20; 25/15; 28/20) fed once per day at 10% of birth weight through weaning. Calves were offered a 20% CP calf starter and water free choice beginning on day 5. All calves were weaned on day 42. Body weights were measured at birth and weekly through weaning. Additionally, hip height, wither height, and …
The Isolation And Characterization Of Bovine Adult Derived Adipose Stem Cells For The Use In Nuclear Transfer, Alicia A. Picou
The Isolation And Characterization Of Bovine Adult Derived Adipose Stem Cells For The Use In Nuclear Transfer, Alicia A. Picou
LSU Master's Theses
Since the cloning of Dolly there has been little change in the efficiency of nuclear transfer (NT). Research is beginning to investigate the characteristics of donor cells. Adiposetissue is an abundant source of adult-derived cells that have displayed “stemness” in-vitro(Gimble et al., 2003). The overall goal of this research was to define the in-vitro characteristicsof bovine adipose-derived adult stem cells (ADAS) for the use in NT. Isolation methods weredetermined by a 3 x 3 factorial design. 1 g of subcutaneous fat was collected and subjected to0.10%, 0.25% or 0.50% collagenase type I solution for 1, 2 and 3 h. Nucleated …
Characterization Of Porcine Adipose Tissue-Derived Stem Cells, Kellie J. Williams
Characterization Of Porcine Adipose Tissue-Derived Stem Cells, Kellie J. Williams
LSU Doctoral Dissertations
Nuclear transfer (NT) results in low success rates and research has reported problems with the welfare of cloned animals produced. Evidence suggests the use of a less differentiated donor cell, such as a stem cell, may lead to an increase in the efficiency of the NT procedure. Stem cell characteristics must be clearly defined in order to isolate, identify and optimally culture potential donor cells. Stem cell characteristics such as self-renewal and differentiation have not been identified in stem cells from porcine adipose tissue, an easily attainable tissue resource. The phenotypic profile of cell surface proteins and chromosomal stability of …
The Interactive Effects Of Phosporus And Calcium On Phosphorus Requirement, Utilization, And Phytase Efficacy In Poultry, Syrena Powell
The Interactive Effects Of Phosporus And Calcium On Phosphorus Requirement, Utilization, And Phytase Efficacy In Poultry, Syrena Powell
LSU Doctoral Dissertations
This research was conducted with broilers to investigate the interactive effects of P and Ca on P requirement, digestibility, utilization, and phytase efficacy. Each treatment had a minimum of 6 replications. One experiment with 3 trials was conducted to identify the nonphytate P (nPP) requirement of 0 to 14 d old broilers. The nPP levels fed ranged from 0.30 to 0.60% with a Ca:nPP of 2.22:1 or 1.9:1. Requirement estimates for 0 to 14 d ranged from 0.52 to 0.60% nPP. One experiment with 2 trials was conducted to identify the nPP requirement of 14 to 28 d old broilers. …
Hatchability Of End Of Lay Egg Production Broiler Breeder Eggs As Influenced By Prestorage Warming, Kyle Homan
Hatchability Of End Of Lay Egg Production Broiler Breeder Eggs As Influenced By Prestorage Warming, Kyle Homan
Honors Capstones
No abstract provided.
The Influence Of Calf Selenium Status On Gpx-1 And 3 Activity And Liver Gpx-1 Mrna, Genevieve Elizabeth Tanner
The Influence Of Calf Selenium Status On Gpx-1 And 3 Activity And Liver Gpx-1 Mrna, Genevieve Elizabeth Tanner
LSU Master's Theses
The purpose of this research was to determine the influence of dietary Se on glutathione peroxidase (GPx)-1 and 3 activities and relative liver GPx mRNA levels in growing Holstein bull calves. Calves (n = 14) were started 28 d after birth on either a Se adequate (0.15 ppm Se) or deficient (0.01 ppm Se) diet consisting of 3 growth phases and maintained on the diet until 180 d of age. Blood samples were taken from each calf for determination of GPx-1 and GPx-3 activity. Three calves were euthanized at d 21 of age for determination of baseline liver GPx-1 mRNA …
Effects Of Probiotics And Yeast Culture On Rumen Development And Growth Of Dairy Calves, Jennifer Marie Laborde
Effects Of Probiotics And Yeast Culture On Rumen Development And Growth Of Dairy Calves, Jennifer Marie Laborde
LSU Master's Theses
Forty-eight calves (heifers n=20, bulls n=28) were randomly assigned one of four dietary treatments which included no additive (C); the yeast culture Saccharomyces cerevisiae (YC); the probiotics Bacillus lichenformis and Bacillus subtilis (P); and both yeast culture and probiotics (YCP). Calves were administered treatments from day 2 to 56 in starter and from day 57 to 84 in grower diets. Body weights were measured at birth and weekly thereafter until day 112 of age. Wither and hip heights were measured weekly. Feed intake, water intake, and fecal scores were recorded twice daily until day 56. Rumen fluid was collected on …
Hatchability Of Post-Peak Egg Production Broiler Breeder Eggs As Influenced By Pre-Incubation Warming, Cameron Benjamin Wiggins Ii
Hatchability Of Post-Peak Egg Production Broiler Breeder Eggs As Influenced By Pre-Incubation Warming, Cameron Benjamin Wiggins Ii
LSU Master's Theses
This research was conducted to determine the effects of pre-incubation warming on the hatchability of post-peak egg production broiler breeder eggs. An experiment with six trials was conducted with 7,920 freshly laid eggs from Ross 308 and 708 broiler breeders from 61-67-wks of age. For each trial, 1,320 eggs were used to determine if pre-incubation warming treatments of 0, 2, 4, and 6, or 0, 3, 6, and 9, or 0, 9, 12 and 15 hrs (at 37.6°C) could improve the hatchability of eggs stored (at 15.5°C) for three days. After a storage period of three days, the eggs were …
The Use Of Serum Uric Acid As An Indicator Of Amino Acid Utilization In Diets For Broilers, Amanda L. Donsbough
The Use Of Serum Uric Acid As An Indicator Of Amino Acid Utilization In Diets For Broilers, Amanda L. Donsbough
LSU Master's Theses
The purpose of this research was to determine if serum uric acid (SUA) could be used as an indicator of amino acid (AA) utilization in broilers and to determine the Lys requirement of broilers using SUA as a response variable. Treatments were replicated with at least 6 pens with at least 6 broilers per pen. Experiments (Exp.) were conducted from 0- to 14, 17, or 18- d posthatching in brooder batteries. Five experiments were conducted to determine if SUA and uric acid content of the excreta (UAE) could be used to determine AA adequacy of a diet. The results of …
Evaluation Of Novel Dietary Feed Additives For Nursery And Growing/Finishing Pigs, Victor D. Naranjo
Evaluation Of Novel Dietary Feed Additives For Nursery And Growing/Finishing Pigs, Victor D. Naranjo
LSU Master's Theses
The objectives of this research were: 1) to compare the effect of source and level of inclusion of dried whey permeate (DWP) and a carbohydrate product (CHO) on growth performance of nursery pigs, 2) to evaluate the effect of a fortified nutrient pack (FNP) on growth performance and total tract nutrient digestibility in growing/finishing pigs, and 3) to compare chromic oxide (Cr2O3) and acid insoluble ash (AIA) as digestibility markers for total tract digestibility determination in growing/finishing pigs. Three experiments were conducted to compare the feeding value of DWP (80% lactose) and CHO (40% lactose, 30% sucrose, and 10% glucose) …
Maternal Corticosterone Influences On Fear Responses In Offspring Of Quail Divergent Stress Response Lines, Kyndal A. Davis
Maternal Corticosterone Influences On Fear Responses In Offspring Of Quail Divergent Stress Response Lines, Kyndal A. Davis
LSU Master's Theses
Increased fearfulness has been associated with adrenocortical activation. Maternal corticosterone (B) treatment increases egg yolk B, and elevated B in ovo enhances chick avoidance of humans. Quail selected for exaggerated (high stress, HS) as opposed to reduced (low stress, LS) plasma B response to stress are more fearful, and more B is found in the egg yolks of HS than LS hens. Therefore, the underlying fearfulness (tonic immobility, TI) and timidity (hole-in-the-wall box, HWB, emergence) responses were assessed in chicks hatched from eggs of LS and HS hens implanted with silastic tubes containing no B (CON) or B (B-IMPLANT) during …
The Utilization Of Red Blood Cells In Diets For Swine And Poultry, Emily Dawn Frugé
The Utilization Of Red Blood Cells In Diets For Swine And Poultry, Emily Dawn Frugé
LSU Master's Theses
The purpose of this research was to determine if increasing levels of RBC would affect growth performance and carcass characteristics of finishing pigs and growth performance of broilers. Three experiments were conducted to determine the effect of incremental levels of red blood cells (RBC; 0 to 4% and 0 to 2%), and thus increasing levels of dietary Leu on growth performance and linear carcass measurements of finishing pigs. Our results suggest that feeding 3 or 4% RBC causes a decrease in growth performance. However, feeding 1 or 2% RBC in the diets of finishing pigs had no detrimental effects on …
Distributions And Associations Of Single Nucleotide Polymorphisms In The Leptin Gene Of Bos Taurus And Bos Indicus Cattle, Douglas Henry Fischer, Jr
Distributions And Associations Of Single Nucleotide Polymorphisms In The Leptin Gene Of Bos Taurus And Bos Indicus Cattle, Douglas Henry Fischer, Jr
LSU Master's Theses
In recent years, the use of genetic markers has become more and more prevalent in beef breeding programs. This research focused on four previously identified single nucleotide polymorphisms in a leptin gene on chromosome 4 of beef cows. The SNP were E2FB, T945M, UA1, and UA2. Beef cows used in this research were maintained at the Louisiana State University AgCenter Central Research Station. Cows consisted of purebred Bos tauras and Bos indicus cattle as well as crossbreds. The objectives were to estimate genotypic and allelic frequencies for each SNP and to determine the influence of cow breed type, cow age, …
Influences Of Maternal Corticosterone On Incubation Length And Hatchability Of Eggs Laid By Quail Hens Selected For Divergent Adrenocortical Stress Responsiveness, Jason Berante' Schmidt
Influences Of Maternal Corticosterone On Incubation Length And Hatchability Of Eggs Laid By Quail Hens Selected For Divergent Adrenocortical Stress Responsiveness, Jason Berante' Schmidt
LSU Master's Theses
Unstressed and stressed quail hens selected for exaggerated (HS, high stress) rather than reduced (LS, low stress) plasma corticosterone (B) response to brief restraint deposit more B into their eggs than do their LS hen counterparts. HS hens implanted with B also show reduced egg lay when compared to LS- and HS-control and LS-B-implanted hens. Herein, the effects of stress line on length of egg incubation (LEI) and chick body weight at emergence (BWTE) (Exp. 1) and the interactive influences of line with maternal B-treatment (sub-Q control, CON-, or B-implants) on LEI (Exp. 2) and on egg fertility (FERT), total …
Effects Of Protein Sources On Growth And Hormonal Status Of Weaned Dairy Calves, Christopher Aaron Sissell
Effects Of Protein Sources On Growth And Hormonal Status Of Weaned Dairy Calves, Christopher Aaron Sissell
LSU Master's Theses
Eight Holstein calves approximately 6 months of age (mean BW 185.15 + 16.16 kg) were used in a replicated 4 x 4 Latin Square experiment to study effects of protein sources on performance of weaned dairy calves. Dietary treatments consisted of 16% CP diets with three sources of ruminally undegradable protein (RUP). Experimental diets were corn-silage based, with soybean meal (SBM) as the source of ruminally degradable protein (control) and 3 sources of RUP including heat treated soybean meal (SBM) (SoyPLUS®), animal protein blend (PRO-LAK), and extruded-expelled SBM, included at 45% of the dietary CP. The animals were fed their …
Effect Of Culture Conditions On Gene Expression In Manipulated Bovine Embryos, Megan Nicole Purpera
Effect Of Culture Conditions On Gene Expression In Manipulated Bovine Embryos, Megan Nicole Purpera
LSU Master's Theses
Numerous studies have reported aberrant gene expression levels attributed to suboptimal in vitro culture conditions presented to embryos. Since the culture environment is a common aspect of both in vitro production (IVP) and nuclear transfer (NT), research focusing on the in vitro culture system will have the potential to improve both techniques. This study investigated the effects of different culture systems and protein sources on the developmental competence of IVP embryos measured by cleavage and blastocyst rates, cell number, and relative abundance of oct-4, nanog, connexin 43, and GLUT-1 transcripts when compared to in vivo embryos. Experiment 1 compared IVP …
Equine Obesity-Related Hyperleptinemia, Nan Killen Huff
Equine Obesity-Related Hyperleptinemia, Nan Killen Huff
LSU Doctoral Dissertations
Plasma leptin concentrations in obese adult horses have been shown to vary widely, and horses tend to fit into two groups: low leptin (<10 ng/mL) and hyperleptinemic (10 to 50 ng/mL). Observations over time revealed that the hyperleptinemic condition was consistent, possibly indicating a relatively permanent underlying cause. Based on these observations, three experimental approaches were used to further study equine obesity-related hyperleptinemia. The first experiment determined the prevalence of hyperleptinemia among postpartum, lactating mares, evaluated its consequence on their re-breeding success, and investigated correlations between leptin levels in lactating and non-lactating mares. Postpartum mares (n = 198) and non-foaling mares (n = 31) were categorized based on their leptin status: normoleptinemic or hyperleptinemic. Leptin in the lactating mares averaged 4.8 ng/mL, and 11 of the 198 (13%) displayed hyperleptinemia. Leptin in the non-lactating mares averaged 7.5 ng/mL, with 9 mares (29%) displaying hyperleptinemia. Of the 198 lactating mares bred, 81% became pregnant; there was no effect of leptin status on re-breeding success. To study one possible cause for hyperleptinemia in well-fed horses, a second experiment explored polymorphism(s) within exon 2 of the equine leptin gene. The DNA from five hyperleptinemic and five normal mares of high body condition was used to analyze exon 2 of the leptin gene for polymorphisms. Based on the 10 mares tested, there was no polymorphism in exon 2 of the equine leptin gene; therefore, polymorphism is not a likely explanation for the high vs. low leptin difference. The third experiment explored the possible effects of hyperleptinemia on the endocrine and immune systems. Endotoxin was given to mares and geldings to investigate the role and/or regulation of leptin in the pro-inflammatory cytokine response. Of the endpoints measured, only platelet count differed between normal and hyperleptinemic horses. Endotoxin infusion caused the expected pro-inflammatory cytokine and endocrine responses, but leptin status was not a significant factor for any endpoint. It is concluded that hyperleptinemia in mares is not associated with polymorphism in exon 2 of the leptin gene, does not affect re-breeding rates of foaling mares, and does not alter the endotoxin-induced responses of the endocrine and immune systems.
In Vitro Development Of Bovine Embryos Cultured In A Frozen-Thawed Commercial Culture Medium, Jeanne Lee Glaser
In Vitro Development Of Bovine Embryos Cultured In A Frozen-Thawed Commercial Culture Medium, Jeanne Lee Glaser
LSU Master's Theses
As in vitro fertilization (IVF) becomes more acceptable for the treatment of human infertility, it is essential for clinics to have in place an optimal embryo culture system. The objective of this study was to evaluate whether freezing and storing human embryo culture medium will alter embryo post-thaw viability. In consecutive experiments, commercial culture medium was frozen at -20°C and at -80°C and stored at 4°C until its use in IVF. Each IVF-derived replicate of bovine embryos contained a laboratory control group, a fresh commercial culture medium group(s) and a frozen-thawed commercial culture medium group(s). The commercial culture medium was …
Cryopreservation Of White-Tail Deer Epididymal Sperm For Artificial Insemination, Jesse Ray Saenz
Cryopreservation Of White-Tail Deer Epididymal Sperm For Artificial Insemination, Jesse Ray Saenz
LSU Master's Theses
The ability to cryopreserve epididymal sperm from mature postmortem bucks has long been of interest to both wildlife conservationists and deer ranchers. Increased understanding of the cryobiology of epididymal sperm from a non domestic species, such as White-tail deer, could aid in development of future protocols to assist in the preservation of endangered species. In Experiment I, results showed that after cooling postmortem bull testes for 22 hours, no significant difference was noted between sperm parameters of epididymal sperm collected at room temperature or at a cool enviorment. In Experiment II, it was shown that White-tail deer sperm could be …
Reproductive And Metabolic Effects Of Recombinant Equine Leptin On Seasonally Anovulatory Mares, Pamela Boliew Mitcham
Reproductive And Metabolic Effects Of Recombinant Equine Leptin On Seasonally Anovulatory Mares, Pamela Boliew Mitcham
LSU Master's Theses
Mares of poor body condition have low plasma leptin concentrations, while obese mares have the highest concentrations. Leptin is a primary signal of body condition to the brain in other species; therefore, low leptin concentrations in thin mares could contribute to their extended anovulatory period in winter compared to obese mares. The current experiment was designed to determine whether recombinant equine leptin, administered to seasonally anovulatory mares, would induce ovarian activity and ovulation in the winter. Leptin effects on metabolism were also studied. Beginning January 7, leptin-treated mares (n = 9) received daily i.m. injections of 10 mg recombinant equine …
The Effects Of Various Feed Additives On Growth Performance Of Nursery Pigs, Jennifer Tucker
The Effects Of Various Feed Additives On Growth Performance Of Nursery Pigs, Jennifer Tucker
LSU Master's Theses
The objective of this research was to evaluate the effects of various feed additives on growth performance of nursery pigs. An experiment was conducted in which pigs were fed a conventional Phase 1 diet containing: 1) no salmon protein hydrolysate (SPH) or spray-dried porcine plasma (SDPP); 2-3) 1.5% SPH or SDPP; 4-5) 3.0% SPH or SDPP; or 6) 1.5% SPH and 1.5% SDPP. The results of this experiment showed growth performance was unaffected by source or level of protein during any growth phase. Overall gain:feed was greater (P = 0.08) for pigs fed the 1.5% level of protein than for …
Intracellular Lipids In Bos Indicus And Box Taurus Oocytes, Casey Bryant Ballard
Intracellular Lipids In Bos Indicus And Box Taurus Oocytes, Casey Bryant Ballard
LSU Master's Theses
It has been proposed that Bos indicus when compared with Bos taurus embryos are more sensitive to cryopreservation procedures due to higher intracellular lipid content of the embryos. With cyropreservation of Bos indicus embryos increasing for export, it is essential to improve the viability of these cryopreserved embryos. In Experiment I, M-II oocytes from mice, pigs and cows were subjected to two different procedures to evaluate lipid content. The lipid content present in pig oocytes was significantly (P<0.05) higher when compared with mouse and cow oocytes. Pig oocytes had a lower settling point in the sucrose buoyant density gradient and higher Nile Red units, which indicated higher lipids present in the oocytes. In Experiment II, it was determined that M-II oocytes aspirated from superstimulated Brahman donors were significantly higher (P<0.05) in lipid content compared with English breed donors. The lipid content present in Brahman oocytes was higher when compared with English breeds when tested with a sucrose buoyant density gradient and Nile Red staining. In addition, donor females with higher body condition scores were significantly (P<0.05) higher in oocyte lipid content when compared with donor females of lower body condition scores. Furthermore, animal body condition scores, cholesterol and triglyceride levels prior to oocyte aspiration may be indicators of intracellular lipids in the oocytes and embryos. To our knowledge, this is the first report that evaluated intracellular lipid content of M-II oocytes of different beef breed types using a sucrose buoyant density gradient and Nile Red staining.
Low Crude Protein, Amino Acid-Supplemented Diets, And The Glycine Requirement In Low Crude Protein Diets For Broilers, April Marie Waguespack
Low Crude Protein, Amino Acid-Supplemented Diets, And The Glycine Requirement In Low Crude Protein Diets For Broilers, April Marie Waguespack
LSU Master's Theses
The purpose of this research was to determine the optimal level of crystalline amino acid (AA) supplementation that supports maximum growth performance in broilers, and to determine the Gly requirement in broilers. Treatments were replicated with a minimum of 7 pens with 6 broilers per pen. Experiments (Exp.) were conducted from 0- to 18- days (d) post-hatching in brooder batteries. Three Exp. were conducted to determine the maximum level of L-Lys•HCl that could be supplemented to corn-soybean meal (C-SBM) diets without negatively affecting growth performance of broilers. The results of these Exp. indicate that broilers can achieve maximum growth performance …
Effect Of Age, Body Condition, Pregnancy And Lactation On Circulating Leptin Concentrations In Beef Cattle, Glen Talmage Gentry, Jr.
Effect Of Age, Body Condition, Pregnancy And Lactation On Circulating Leptin Concentrations In Beef Cattle, Glen Talmage Gentry, Jr.
LSU Doctoral Dissertations
A series of experiments were conducted to evaluate the potential role of leptin in bovine reproduction. In Experiment 1, mean circulating leptin concentrations of postpartum cows were not affected by exogenous dexamethasone treatments. In Experiment 2, mean leptin concentrations were not correlated with female age or body weight but were positively correlated with body condition scores of beef cattle. Leptin concentrations were higher in 1 year old heifers (8.9 ng/ml) compared with 2 year old cows (6.0 ng/ml), but heifer leptin concentrations were not different than 4 to 6 year old cows (8.0 ng/ml) and cows ≥7 years of age …
In Utero And In Vitro Sex Ratio Of Bovine Embryos And Calves Originating From The Left And Right Ovaries, Darin Alan Hylan
In Utero And In Vitro Sex Ratio Of Bovine Embryos And Calves Originating From The Left And Right Ovaries, Darin Alan Hylan
LSU Doctoral Dissertations
An asymmetric distribution of the sexes within the left and right uterine horns has been described in multiple polytocous, laboratory species. A series of experiments were conducted to evaluate the sex ratio (% male) of calves gestated in the left and right uterine horns, as well as the sex ratio of embryos originating from the left and right ovaries of cattle. In Experiment 1, the sex ratio of calves and fetuses gestated in the left and right uterine horns was investigated. The sex ratio of calves and fetuses gestated in the right uterine horn was significantly higher compared with the …
Effects Of Cryopreservation And Constituents Of Semen Extenders On Mitochondrial Function Of Bull Spermatozoa, Abdulhakeem Hashim Eljarah
Effects Of Cryopreservation And Constituents Of Semen Extenders On Mitochondrial Function Of Bull Spermatozoa, Abdulhakeem Hashim Eljarah
LSU Doctoral Dissertations
This study investigated the effects of semen extender constituents and cryopreservation on bovine spermatozoal mitochondrial function. Three yearling Holstein bulls were used. Two ejaculates per bull were collected and pooled on a weekly basis for five weeks and extended in four treatments: 1) sodium citrate egg yolk extender with antibiotics (lincomycin, spectinomycin, gentamicin and tylosin); 2) ¡°1¡± with glycerol; 3) ¡°2¡± without antibiotics; and 4) ¡°1¡± without antibiotics. Each was divided into portions for analyses before freezing and after cryopreservation. The pre-freeze and thawed semen were transferred to a 37¡ãC water bath, the same assays were performed. In experiment 1, …
Chromosomal Stability And Epigenetic Modifications Of Fibroblast Cells Used For Nuclear Transfer, Angelica Maria Giraldo Gomez
Chromosomal Stability And Epigenetic Modifications Of Fibroblast Cells Used For Nuclear Transfer, Angelica Maria Giraldo Gomez
LSU Doctoral Dissertations
Investigations into the importance of the nuclear integrity of the donor cell prior to nuclear transfer (NT) are limited. In Experiment 1, the proliferative characteristics, chromosomal stability and level of histone phosphorylation in cell lines established by explants and enzymatic dissociation at different population doublings (PDs) were investigated. The cells divided at a constant rate and cell cycle length increased only at the end of the proliferative stage. The level of aneuploidies was high and remained elevated throughout the study independent of the technique used to establish the primary culture. High levels of multinucleated cells and abnormal spindle configurations were …
Evaluation Of Bovine Spermatid Sex Ratio By Fluorescent In Situ Hybridization, Michael Alan Stout
Evaluation Of Bovine Spermatid Sex Ratio By Fluorescent In Situ Hybridization, Michael Alan Stout
LSU Master's Theses
The objective of this study was to use fluorescent in situ hybridization (FISH) to determine if a skew in the sex ratio was present at the level of the bovine round spermatid. Paraffin embedded bovine testicular tissue obtained from biopsies were used to perform FISH, and the seminiferous tubules were examined. Our findings show that the skew does exist within the round spermatids. Analysis of variance on X- and Y-chromosome bearing round spermatids as affected by sex, slide, picture set, and tubule was performed. Sex showed that the variation in round spermatid sex ratio differed between slides (P = 0.0001). …
Estradiol Interactions With Dopamine Antagonists In Mares: Prolactin Secretion And Reproductive Traits, Kristian Kandis Kelley
Estradiol Interactions With Dopamine Antagonists In Mares: Prolactin Secretion And Reproductive Traits, Kristian Kandis Kelley
LSU Master's Theses
Two experiments studied the effects of pretreatment with estradiol benzoate before treatment with a dopamine antagonist on prolactin secretion and reproductive traits in mares during 1) the seasonal anovulatory period and 2) the normal breeding season. Experiment 1 was performed in winter with 17 mares selected for low follicular activity. Nine mares received estradiol benzoate injections every other day for a total of 10 injections; 8 mares received similar injections of vehicle. Ten days after onset of injections, all mares were placed on a daily injections of sulpiride (250 mg) for 45 d or until ovulation. Plasma prolactin concentrations were …