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Articles 241 - 270 of 296

Full-Text Articles in Animal Sciences

Leptin In Horses: Influences Of Body Condition, Gender, Insulin Insensitivity, Feeding, And Dexamethasone, Joshua Allen Cartmill Jan 2004

Leptin In Horses: Influences Of Body Condition, Gender, Insulin Insensitivity, Feeding, And Dexamethasone, Joshua Allen Cartmill

LSU Doctoral Dissertations

The experiments described herein were designed to answer questions that arose from initially attempting to determine whether treatment with dexamethasone increased concentrations of leptin in geldings. Dexamethasone treatment did in fact increase leptin concentrations in mares, geldings, and stallions. Additional experiments were designed to determine leptin's interaction, not only with the adrenal axis, but with the thyroid axis, the growth hormone/insulin-like growth factor-1 axis, as well as glucose and insulin metabolism in geldings, mares, and stallions. During the course of these experiments, differences in leptin concentrations in the horse were attributed to degree of body condition, gender, and feeding time. …

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The Effects Of Inorganic And Organic Selenium Sources On Growth Performance, Carcass Traits, Tissue Mineral Concentrations, And Enzyme Activity In Poultry, Robert L, Iii Payne Jan 2004

The Effects Of Inorganic And Organic Selenium Sources On Growth Performance, Carcass Traits, Tissue Mineral Concentrations, And Enzyme Activity In Poultry, Robert L, Iii Payne

LSU Doctoral Dissertations

The purpose of this research was to compare dietary inorganic and organic Se sources in poultry. An experiment was conducted to assess daily egg production and Se deposition in eggs of hens fed diets supplemented with inorganic or organic Se. The results from this experiment indicated that hen production was not affected by Se source, and organic Se increased egg Se concentrations more than inorganic Se. Two experiments then were conducted with broilers to compare dietary organic and inorganic Se on growth performance, carcass traits, breast and plasma Se concentrations, and plasma glutathione peroxidase activity. The results from these experiments …

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The Non-Phosphorus Effects Of Dietary Phytase In Swine And Poultry, Jason Layne Shelton Jan 2004

The Non-Phosphorus Effects Of Dietary Phytase In Swine And Poultry, Jason Layne Shelton

LSU Doctoral Dissertations

This research was conducted to determine the non-phosphorus effects of phytase in diets for swine and poultry. An experiment was conducted to determine the effect of phytase addition on energy availability and protein and fat deposition in growing pigs. Results from this experiment indicated that phytase had small positive effects on energy availability, protein deposition, and fat deposition. In this study, 23 of 29 response variables were at least numerically increased with phytase addition. Two experiments were conducted to determine the accuracy of the energy and amino acid matrix values for Natuphos 1200 in broilers from 0 to 21 or …

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The Chick Embryo Amnion As An In Vitro Culture System For Ivf And Nt Embryos, Tonya Renea Davidson Jan 2004

The Chick Embryo Amnion As An In Vitro Culture System For Ivf And Nt Embryos, Tonya Renea Davidson

LSU Doctoral Dissertations

Calving rates are significantly reduced following in vitro production of embryos. Thus, if a technique could be developed that would increase calving rates by as little as one viable offspring, significant research advances could be made. Therefore, in a series of experiments, the efficiency and quality of culturing IVP bovine embryos in the amnion of a domestic chicken egg was tested. In Experiment I, by culturing IVP bovine embryos in the chick amnion (day 4 to 7 of incubation) it was discovered that there was no significant difference in blastocyst rates compared with controls. In Experiment II, it was shown …

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Preservation Of Sperm Harvested From The Rat, Caprine, Equine, And Bovine Epididymis, Aida Nioma James Jan 2004

Preservation Of Sperm Harvested From The Rat, Caprine, Equine, And Bovine Epididymis, Aida Nioma James

LSU Doctoral Dissertations

The interest in preserving endangered species has increased the amount of attention lent to the recovery of functional sperm from the epididymides of deceased males (Foote, 2000). Postmortem specimens have a finite time period before decomposition affects functionality. Determination of this “window of opportunity” to harvest and preserve epididymal sperm would be beneficial for further research in sperm preservation and assisted reproductive technologies. The objective of these studies was to determine 1) the “window of opportunity” to collect viable rat, caprine, equine and bovine epididymal sperm, 2) if epididymal sperm collected could be cryopreserved, 3) to test two common cryoprotectants …

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Somatic Cell Interspecies Nuclear Transfer, Marina Julia Sansinena Jan 2004

Somatic Cell Interspecies Nuclear Transfer, Marina Julia Sansinena

LSU Doctoral Dissertations

The low efficiency of the nuclear transfer (NT) procedure requires large number of oocytes to produce embryos and live offspring. A series of experiments were conducted to evaluate the ability of the bovine cytoplast to reprogram nuclei from horses and llamas. In a preliminary study, equine oocytes from small (<20mm diameter) follicles were either pretreated with roscovitine or placed in maturation (IVM only) prior to NT. Roscovitine pretreatment did not improve nuclear maturation rates (roscovitine pretreatment 57% vs. IVM only 66%) and no fusion was obtained from roscovitine-pretreated oocytes after NT. Another preliminary study was conducted with the objective to produce llama NT embryos and to compare their development in two in vitro culture conditions (G1.2® vs. CR1aa). No difference was found in the number of embryos cleaved after 2 d of culture. This resulted in the first scientific report of somatic cell NT, in vitro culture and transfer of NT embryos in the llama. In the next experiment, adult horse and llama fibroblasts were injected into enucleated cow oocytes. The results showed the cow cytoplasm is capable of partially reprogramming nuclei from other species and support mitotic divisions. However, this study also showed a consistent embryonic developmental arrest at the 8- to 16- cell stage when horse or llama donor cells were used as donor nuclei. When a more closely related species of donor cell (banteng) and recipient oocyte (domestic cattle) were used for NT, no embryonic developmental arrest was found. Embryos progressed to achieve high blastocyst rates (banteng male cell line 28% vs. banteng female cell line 15%). Two banteng interspecies NT pregnancies were established and subsequently lost from the banteng male cell line. In the final study, the effect of a mixed mitochondrial population (heteroplasmy) on early embryonic development was investigated. Ooplasmic transfer performed in combination with NT procedure indicated presence of foreign mitochondria clustered in a small portion of the cytoplasm in early stages of embryo development. When goat ooplasm was transferred into interspecies (cow oocyte-goat donor cell) NT embryos, fusion and cleave rates were reduced suggesting an increased level of heteroplasmy or nuclear-ooplasmic incompatibilities.

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Enhancement Of Ehrlichia Canis Infection Of Canine Blood Monocytes In Vitro Through The Addition Of Seropositive And Monoclonal Antibody, John Paul Gniady May 2003

Enhancement Of Ehrlichia Canis Infection Of Canine Blood Monocytes In Vitro Through The Addition Of Seropositive And Monoclonal Antibody, John Paul Gniady

Honors Capstones

No abstract provided.

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Effects Of Free Methionine And Lysine On In Vitro Fermentation And In Vivo Performance And Ruminal Fermentation Of Late Lactation Holstein Cows, Yi-Hua Chung Jan 2003

Effects Of Free Methionine And Lysine On In Vitro Fermentation And In Vivo Performance And Ruminal Fermentation Of Late Lactation Holstein Cows, Yi-Hua Chung

LSU Master's Theses

Two experiments were conducted to investigate the effects of free Met and Lys supplementation on ruminal fermentation in vitro and in vivo. In the in vitro study, supplementation of Met and Lys had effects on concentrations of total VFA and NH4+, proportions of acetate, propionate, butyrate, isovalerate, valerate, and the ratio of acetate to propionate. Percentages of microbial N synthesis in effluent pellets were not affected by supplementing Lys and Met. The combination of 0.52% Met and 1.03% (90% DM) Lys resulted in the highest concentration of total VFA, second highest concentration of NH4+, …

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Use Of Urine Ph To Predict Incidence Of Ketosis In Transition Dairy Cows, Amy Elizabeth Beem Jan 2003

Use Of Urine Ph To Predict Incidence Of Ketosis In Transition Dairy Cows, Amy Elizabeth Beem

LSU Master's Theses

Forty-one (10 primiparous and 31 multiparous) Holstein cows were used in an experiment to determine the effects of prepartum dietary energy (PDE) and supplemental Ca-propionate on the incidence of ketosis in transition dairy cows and to evaluate the usefulness of urine pH as a predictor for ketosis. Treatment factors were prepartum dietary energy concentration (normal vs. high) with or without 113.5g Ca-Propionate/day (Nutro Cal®, Kemin Industries, Inc., Des Moines, IA). All cows were fed the same basal diet postpartum. Ca-propionate supplementation continued postpartum. Cows were individually fed and dry matter intakes (DMI) were measured daily. Milk production was measured and …

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Cryopreservation By Pellet Freezing Of Epididymal And Ejaculated Spermatozoa From Male Dogs, Brooke Fahrig Jan 2003

Cryopreservation By Pellet Freezing Of Epididymal And Ejaculated Spermatozoa From Male Dogs, Brooke Fahrig

LSU Master's Theses

In this study, I evaluated the cryopreservation by pellet freezing of spermatozoa from individual dogs. In Experiment I, spermatozoa from 15 pairs of epididymides were suspended in glycerol, frozen as pellets of 10, 50, 100, or 200 μl volumes, and thawed by dilution with TALP (Tyrode's solution plus albumin, lactate, pyruvate). In Experiment II, spermatozoa from 16 pairs of epididymides were suspended in glycerol, dimethyl sulfoxide, or ethylene glycol, frozen as 100 μl pellets, and thawed by dilution with TALP, canine capacitation medium (CCM), or 3% sodium citrate solution. In Experiment III, spermatozoa from 15 pairs of epididymides were suspended …

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The Effects Of Composition And Processing Of Milk On Foam Characteristics As Measured By Steam Frothing, Michael Levy Jan 2003

The Effects Of Composition And Processing Of Milk On Foam Characteristics As Measured By Steam Frothing, Michael Levy

LSU Master's Theses

Steam frothing of milk is required to produce an acceptable foam for many espresso coffee drinks. Specific aspects of composition and processing may affect the foaming properties of milk. The aim of this study was to determine the effect of fat content, heat treatment, free fatty acid addition and storage time on the frothing properties of milk. The four treatments included: fat content (0.08% and 3.25%), pasteurization temperatures (171°F for 15 seconds and 210°F for 45 seconds), pre and post-pasteurization addition of lauric acid solution (0.0% and 2.0% of 0.5 M concentration) and storage time (1 and 10 days). For …

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Characteristics Of Fat Free Yogurt As Influenced By The Incorporation Of Folic Acid, Charles August Boeneke Jan 2003

Characteristics Of Fat Free Yogurt As Influenced By The Incorporation Of Folic Acid, Charles August Boeneke

LSU Doctoral Dissertations

Folic acid fortification is used in the prevention of neural tube defects such as spina bifida and ancencephaly, heart defects, facial clefts, urinary tract abnormalities, and limb deficiencies. Although yogurt is not a good source of folic acid, fortification could aid in prevention of above mentioned defects. Fortification of yogurt with folic acid may or may not change its physico-chemical characteristics. Fat free sugar free yogurt was manufactured using 0, 25%, 50%, 75% and 100% of the recommended daily allowance of 400 micrograms of folic acid. Treatments included addition of folic acid at these levels before and after pasteurization. Lemon …

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Proportional Assessment Of X And Y Chromosome-Bearing Spermatozoa In Bull And Boar Ejaculates Using Conventional And Real-Time Pcr Techniques, Julius Braden Paul Jan 2003

Proportional Assessment Of X And Y Chromosome-Bearing Spermatozoa In Bull And Boar Ejaculates Using Conventional And Real-Time Pcr Techniques, Julius Braden Paul

LSU Doctoral Dissertations

Considerable variation in the percentage of Y-chromosome bearing spermatozoa (%Y-CBS) between ejaculates in the same male has been reported. Collection regime has been speculated to influence the degree of variation in %Y-CBS in bull ejaculates (Chandler et al., 1998). Experiments were designed to evaluate the effects of two collection regimes on %Y-CBS. Conventional PCR combined with gel electrophoresis and image analysis was employed to determine %Y-CBS in ejaculates from two bulls collected on 7-day intervals and two bulls on 21-day intervals. Real-time PCR technology was used to quantify %Y-CBS in the same ejaculates. Boar ejaculates were also analyzed with both …

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Oocyte Production In The Early Postpartum Cow, Oscar Perez Jan 2003

Oocyte Production In The Early Postpartum Cow, Oscar Perez

LSU Doctoral Dissertations

The postpartum period has been the focus of numerous studies; however, there is no information available relating to oocyte production in early postpartum cows. Ovaries of early postpartum cows were stimulated with FSH to produce follicular development and oocytes. The objectives of these studies were: (1) to evaluate the use of FSH for oocyte production in early postpartum beef cows, (2) to evaluate follicular response and oocyte quality of beef cows treated with FSH shortly after calving, (3) to evaluate FSH for oocyte production in early postpartum dairy cows, (4) to evaluate responses of FSH-treated, early postpartum beef cows in …

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The Louisiana Calf-To-Carcass Program: Growth And Carcass Traits, James E. Devillier Jan 2003

The Louisiana Calf-To-Carcass Program: Growth And Carcass Traits, James E. Devillier

LSU Doctoral Dissertations

Feedlot and carcass data from 1,533 weanling steers consigned to the Louisiana Calf-to-Carcass program from 1992 to 1998 were used to evaluate the influence of feeder calf grade, sire breed, and initial feedlot weight on growth and carcass traits. Each October, spring born calves were delivered to Clinton, Ruston, or Lake Charles loadout sites. Steers were identified by sire breed, tagged, weighed, assigned a feeder calf grade. Forty-six, 18, 26, and 10 percent of the steers graded large frame-thick muscle, large frame-moderate muscle, medium frame-thick muscle, and medium frame-moderate muscle, respectively. Steers were trucked to a commercial feedlot and sorted …

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Population Structure And Genetics Of Longevity In A Colony Of Dog Guides, John B. Cole Jan 2003

Population Structure And Genetics Of Longevity In A Colony Of Dog Guides, John B. Cole

LSU Doctoral Dissertations

The objectives of this study were the description of changes in genetic diversity in a colony of dog guides since its founding, and the investigation of the genetics of longevity in that population. Two breeds of dog, German Shepherds (GS) and Labrador Retrievers (LR), were evaluated. There were rapid increases in average pairwise relationship in both breeds, although the average was approximately one-third higher in the GS population than in the LR population. A similar trend was observed for average inbreeding. In the current generation, relationship and inbreeding for all animals averaged 25.3% and 26.2% in GS and 15.5% and …

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Quantitative Inheritance Of Calpastatin Activity As An Assessment Measure For Meat Tenderness In Brahman Steers, Fuad Mohammad Tawfiq Odeh Jan 2003

Quantitative Inheritance Of Calpastatin Activity As An Assessment Measure For Meat Tenderness In Brahman Steers, Fuad Mohammad Tawfiq Odeh

LSU Doctoral Dissertations

Calpastatin activity at 24 h postmortem is inversely proportional to postmortem tenderization and accounts for a greater proportion of the variation in beef tenderness. Tenderness was determined by measurement of 24 h postmortem longissimus muscle calpastatin activity (CA) and Warner-Bratzler shear force after 7 and 14 d of steak aging. The quantitative inheritance of tenderness in Brahman steers was investigated phenotypically by Multiple Trait Derivative-Free Restricted Maximum Likelihood (MTDFREML) procedures and genotypically by using restriction fragment length polymorphisms (RFLP), a microsatellite marker, and DNA sequence analyses. In experiment 1, MTDFREML was used to obtain the genetic parameters of tenderness traits …

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The Effects Of Egg Positioning On Broiler Breeder Eggs During Incubation On Hatchability And Chick Weight, Brian Tyler Thompson May 2002

The Effects Of Egg Positioning On Broiler Breeder Eggs During Incubation On Hatchability And Chick Weight, Brian Tyler Thompson

Honors Capstones

No abstract provided.

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The Effects Of Albumen Removal On Broiler Breeder Eggs Prior To Incubation On Hatchability And Chick Weight, Charles Sutcliffe May 2002

The Effects Of Albumen Removal On Broiler Breeder Eggs Prior To Incubation On Hatchability And Chick Weight, Charles Sutcliffe

Honors Capstones

No abstract provided.

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The Effects Of Growth Hormone Or Melatonin On The Reproductive Axis Of Stallions, William Andrew Storer Jan 2002

The Effects Of Growth Hormone Or Melatonin On The Reproductive Axis Of Stallions, William Andrew Storer

LSU Master's Theses

Two experiments were conducted to determine the effects of growth hormone (GH) or melatonin on the reproductive axis of the stallion. In Experiment 1, nine stallions were treated with GH (20 µg/kg BW) or saline for 21 d starting in January. During the last week of treatment, stallions were subjected to low and high dose injections of luteinizing hormone (LH), as well as low and high dose combined injections of gonadotropin releasing hormone (GnRH) and thyrotropin releasing hormone (TRH). Two months after the onset of GH treatment, semen was collected from all stallions every other day for 2 weeks. Treatment …

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The Effects Of Phytase In Nutritionally Adequate Diets, Diets Deficient In Calcium And Phosphorus, And The Interactive Effects Of Phytase And Eimeria Acervulina Infection In Broiler Chicks, Brandy Centrell Watson Jan 2002

The Effects Of Phytase In Nutritionally Adequate Diets, Diets Deficient In Calcium And Phosphorus, And The Interactive Effects Of Phytase And Eimeria Acervulina Infection In Broiler Chicks, Brandy Centrell Watson

LSU Master's Theses

Six experiments were conducted to determine the interactive effects of Eimeria acervulina (E. acervulina) infection and phytase, and the effects of phytase in nutritionally adequate diets and in diets deficient in Ca and available P (aP). Corn-soybean meal (C-SBM) diets were used. In Exp 1, treatments were: 1) C-SBM, 1.0% Ca and 0.45% aP; 2) C-SBM, 0.80% Ca and 0.25% aP; 3) Diet 1 + 600 FTU phytase/kg; 4) Diet 2 + 600 FTU phytase/kg; 5 to 8) Diets 1 to 4 but infected with coccidiosis. Weight gain (ADG), feed intake (ADFI), and gain:feed were reduced (P < 0.01) by the coccidial infection and the reduction in Ca and aP. Phytase increased (P < 0.02) ADG and ADFI, regardless of the Ca and aP content of the diet or the presence of coccidiosis. Gain:feed was increased by phytase but only in uninfected chicks (phytase x coccidiosis interaction, P < 0.02). Phytase increased (P < 0.02) bone ash percentage but only in diets deficient in Ca and aP (P < 0.01). Experiments 2 and 3 included only treatments 1 to 4 of Exp 1. The reduction in Ca and aP reduced (P < 0.01) ADG, ADFI, and gain:feed. Phytase addition increased (P < 0.02) ADG and ADFI in diets deficient in Ca and aP and in the nutritionally adequate diets. Experiments 4, 5, and 6 were conducted to determine the effects of phytase on intestinal transit time in broilers. Diets were: 1) C-SBM, 0.9% Ca and 0.35% aP; 2) C-SBM, 0.80% Ca and 0.25% aP + 600 FTU phytase/kg. Transit time on Day 1, but not on Day 7, was faster (P < 0.03) in chicks fed phytase. These data indicate that phytase is effective in the presence of a coccidial infection, but it may not be as effective as in uninfected chicks. Futhermore, phytase increases growth in diets deficient in Ca and aP and in diets formulated to be adequate in all nutrients. This increase in growth may be due to a faster transit time of feed through the digestive tract, resulting in a greater feed intake and gain.

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The Effect Of Nocturnal Sampling On Semen Quality And The Efficiency Of Collection In Bovine Species, Jennifer H. Yates Jan 2002

The Effect Of Nocturnal Sampling On Semen Quality And The Efficiency Of Collection In Bovine Species, Jennifer H. Yates

LSU Master's Theses

The objective of this study was to evaluate two semen collection schedules utilizing the assumed behavioral differences between bovine species. The efficiency was measured by bull behavior and semen quality parameters. Four Holstein and four Brahman bulls were collected each during a morning and a night collection time weekly. Ejaculates (n=64) were obtained via artificial vagina over four-weeks. To avoid masking benefits of night collection, the first collection of the week was alternated between night and day. Two collection teams were employed to reduce the time needed for collection, and the variability in collection method. Sampling order and collection team …

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Follicular Growth And Development And Gonadotropin Response Of Mares Treated With Dihydrotestosterone And Estradiol Benzoate, Scarlett Lynn Mcmeen Jan 2002

Follicular Growth And Development And Gonadotropin Response Of Mares Treated With Dihydrotestosterone And Estradiol Benzoate, Scarlett Lynn Mcmeen

LSU Master's Theses

An experiment was conducted to determine the effects of dihydrotestosterone (DHT) and estradiol benzoate (EB) given simultaneously on concentrations of FSH and LH and the associated follicular activity, and to monitor the subsequent recovery of the gonadotropes and follicular growth following treatment with either DHT or EB. Twelve mares were assigned to daily treatments of DHT (150 Fg/kg BW) plus EB (22 Fg/kg BW) from d 0 through 30 (Phase I). Beginning on d 31, mares were randomly assigned to one of three treatment groups: 1) oil, 2) DHT (150 Fg/kg BW), or 3) EB (22 Fg/kg BW). On d …

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Endocrine And Reproductive Responses To Implants Of Deslorein Acetate In Horses, Carrie Ann Johnson Jan 2002

Endocrine And Reproductive Responses To Implants Of Deslorein Acetate In Horses, Carrie Ann Johnson

LSU Doctoral Dissertations

Four experiments were performed to study the effects of the gonadotropin releasing hormone (GnRH) analog, deslorelin acetate (Ovuplant™), on endocrine and reproductive characteristics in mares. The first experiment tested whether anecdotal field reports of Ovuplant causing extended interovulatory intervals would be detectable under controlled, experimental conditions. The use of Ovuplant to hasten ovulation in 13 mares, compared to 12 controls, increased (P < 0.05) the interovulatory interval by 6.2 d and suppressed (P < 0.05) plasma concentrations of both luteinizing hormone (LH) and follicle stimulating hormone (FSH) for approximately 11 d. Two mares receiving Ovuplant did not return to estrus within 30 d. In the second experiment, 10 control mares and 10 mares induced to ovulate with Ovuplant were administered GnRH (50 μg) on d 1, 4, 7, and 10 after ovulation. Again, treated mares had a longer (4.4 d, P < 0.05) interovulatory interval and suppressed LH and FSH concentrations in daily plasma samples. The gonadotropin response to GnRH was lower (P < 0.05) in the deslorelin mares on d 1, 4, and 7, indicating a lack of pituitary responsiveness. In the third experiment, 9 stallions and 12 steroid-treated geldings were used to determine if males were potential models for studying the deslorelin-induced gonadotropin suppression. In both cases, treatment with Ovuplant caused an initial rise in both gonadotropins followed by suppression for about 14 d. In the last experiment, 21 mares were used to determine if multiple doses of deslorelin would cause complete ovarian shutdown. Mares received either sham injections, three Ovuplant implants on the first day, or one implant per day for 3 d (n = 7 per group). Treatment with multiple deslorelin implants increased (P < 0.05) the interovulatory interval by 14.8 d and suppressed LH and FSH concentrations for approximately 25 d, however no mares exhibited complete ovarian shutdown. In conclusion, deslorelin acetate implants in horses in the form of Ovuplant induce short-term increases in LH and FSH secretion followed by long-term suppression of these concentrations and an insensitivity of the pituitary to GnRH. In a small percentage of mares, long-term ovarian shutdown is a possibility.

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Superfetation In Beef Cattle, Joel Andrew Carter Jan 2002

Superfetation In Beef Cattle, Joel Andrew Carter

LSU Doctoral Dissertations

Although superfetation has been reported in cattle and other species, there is considerable skepticism as to its existence due to the lack of clear evidence in these reports. Other explanations such as embryonic diapause or differential growth of twins have been suggested as more accurate descriptions of the cases reported as superfetation. The hypothesis of this study is that if a viable pregnancy can ensure maternal recognition in cattle, an asynchronous embryo can develop in a more chronologically advanced uterine environment. The objective was to produce superfetation by (1) ovulation induction and artificial insemination (AI) of pregnant cows and (2) …

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Production Of Transgenic Goats By Somatic Cell Nuclear Transfer, Brett C. Reggio Jan 2002

Production Of Transgenic Goats By Somatic Cell Nuclear Transfer, Brett C. Reggio

LSU Doctoral Dissertations

A series of experiments were conducted to produce transgenic goats by somatic cell nuclear transfer (NT). In all experiments, donor cells were electrically fused to enucleated metaphase II oocytes, then chemically activated. In a preliminary study to evaluate embryonic development following NT with proliferating or quiescent fibroblast or cumulus cells, significantly more embryos reconstructed with quiescent cumulus cells fused (77%) compared with proliferating cumulus cells (41%), proliferating fibroblasts (36%) or quiescent fibroblasts (37%). Improved development to the eight- to sixteen-cell stage was observed when fibroblast cells were serum starved (serum starved 39% vs. serum fed 15%). However, there was no …

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Hypoxanthine Phosphoribosyl Transferase And Early Embryo Development, Michael Gerard Collins Ii Jan 2002

Hypoxanthine Phosphoribosyl Transferase And Early Embryo Development, Michael Gerard Collins Ii

LSU Doctoral Dissertations

The objectives of the first experiment were to ascertain if there were any differences between two mouse areola and adipose tissue cell lines. These cell lines differed only in the presence or absence of the hypoxanthine phosphoribosyl transferase (HPRT) gene. It was found that the cell lines were similar for all parameters. The objectives of the second experiment were to determine if the HPRT gene had an effect on the development of preimplantation mouse embryo. To test this transgenic mouse lines were created. The embryo sex and genotype ratio, the embryo diameter, and the embryo developmental patterns, using a Collin-Ryan …

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Nutrient Profile, Functional Properties, And Microstructure Of Dried Waste Milk Products For Use As A Potential Animal Feed, Ronald I. Thompson Jan 2002

Nutrient Profile, Functional Properties, And Microstructure Of Dried Waste Milk Products For Use As A Potential Animal Feed, Ronald I. Thompson

LSU Doctoral Dissertations

A study was conducted to determine the feasibility of concentrating the wastewater of a butter/powder factory for use as an animal feed. Two concentrated products were evaluated: recovered milk product (RMP), which is the direct factory waste stream; and formulated recovered milk product (FRMP), which is a product made by combining RMP and separator de-sludge in a 3:1 ratio. Three pilot scale dryer systems were used to concentrate the product: a spray dryer, a roller dryer, and a pulse combustion dryer. Dried samples were analyzed for fat, moisture, protein, ash, nitrates, chloride, pH, calcium, phosphorus, sodium and amino acids. Values …

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Tryptophan Requirements And The Effects Of Supplemental Tryptophan On Growth Performance, Plasma Metabolites, And Meat Quality In Nursery, Growing, And Finishing Pigs, Amy Christina Guzik Jan 2002

Tryptophan Requirements And The Effects Of Supplemental Tryptophan On Growth Performance, Plasma Metabolites, And Meat Quality In Nursery, Growing, And Finishing Pigs, Amy Christina Guzik

LSU Doctoral Dissertations

This research was conducted to estimate the true digestible Trp (dTrp) requirements in nursery, growing, and late finishing pigs and the effects of supplemental Trp on physiology, behavior, and meat quality. Five experiments were conducted to estimate the dTrp requirement in nursery pigs. Using broken-line regression analysis, dTrp requirements were 0.21, 0.20, and 0.18% for Phase I (5.2 to 7.3 kg), II (6.3 to 10.2 kg), and III (10.3 to 15.7) nursery pigs. In addition, four experiments were conducted to estimate the dTrp requirements in growing and finishing pigs. Using broken-line regression analysis, the dTrp requirement of pigs weighing 30.9, …

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Growth And Endocrine Patterns Of Cloned And Noncloned Brangus Heifers, William Buck Kyle May 2001

Growth And Endocrine Patterns Of Cloned And Noncloned Brangus Heifers, William Buck Kyle

Honors Capstones

No abstract provided.

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