Life Sciences Commons^™

Technological Development Of In-Cell Nmr: Microfluidic, Metabolomic, And Fluxomic Approaches, Nicholas Sciolino

Electronic Theses & Dissertations (2024 - present)

In the fields of structural biology, metabolomics, and fluxomics, there is a driving, technique-based concern when it comes to high-resolution spectrometry and its marriage to biological and physiological relevance. Sample preparation often involves cellular perturbation, lysate separation schemes, combinations with non-biological reagents, and timeline slack. Conceptually, the farther we get from the cell, the greater the potential disparity between what exists in vivo and our in vitro manipulations. This is particularly true when seeking to collect data on transfected targets of interest, and on sensitive metabolic equilibria that occur on the order of minutes. The question remains: if we are …

Conserved A-Rich Regions In The 3’ Utr Of Zika Virus Differentially Affect Viral Gene Expression, Kristen Kaytes

Electronic Theses & Dissertations (2024 - present)

Following its discovery in 1947, Zika virus garnered little attention in the global stage until a rapidly spreading epidemic brough the virus to the global stage in 2015. Continuous advances in research have begun to further the understanding of the molecular biology of Zika virus, however much is still unknown. Much of our understanding of the virus comes from cross reference to other viruses within the same family, such as Dengue virus and West Nile virus. A commonality of these viruses is that they resemble cellular mRNAs and have a single-stranded positive-sense RNA genome that contains a 5’-cap but lacks …

Investigating Rna Dysfunction Of Inherited Neuromuscular And Neurodevelopmental Disease, Jacob H. Schroader

Electronic Theses & Dissertations (2024 - present)

ITPase deficiency is a rare but fatal, autosomal recessive enzyme deficiency involving encephalopathy, microcephaly, congenital cataracts, hypotonia, developmental delay, and dilated cardiomyopathy. Within a decade since the original description of the severe form in humans, there are huge gaps in the field regarding the underlying molecular mechanisms driving pathogenesis resulting from ITPase deficiency. Past studies have focused on characterization of the organism level ITPase-deficient phenotype, but the molecular and cellular characterization remains to be fully described. Due to the biochemical function and established biological role of the ITPase enzyme, investigation into the consequences of inosine accumulation and misincorporation into RNA …

The Complex Development Of Psoralen-Interstrand Crosslink Resistance In Escherichia Coli Requires Acrr Inactivation, Retention Of A MarboxSequence, And One 3 Of Three Mara, Soxs, Or Rob Global Regulators, Travis K. Worley, Ayah H. Asal, Lo Cooper, Charmain T. Courcelle, Justin C. Courcelle

Biology Faculty Publications and Presentations

Crosslinking agents, such as psoralen and UVA radiation, can be effectively used as antimicrobials and for treating several dysplastic conditions in humans, including some cancers. Yet, both cancer cells and bacteria can become resistant to these compounds, making it important to understand how resistance develops. Recently, several mutants were isolated that developed high-levels of resistance to these compounds through upregulation of components of the AcrAB-TolC-efflux pump. Here, we characterized these mutants and found that resistance specifically requires inactivating mutations of the acrR transcriptional repressor which also retain the marbox sequence found within this coding region. In addition, the …

Heterospecific Coprophagy In The White-Tailed Deer Odocoileus Virginianus (Zimmermann, 1780) In Costa Rica, Mora Manuel Jose, Juan De Dios Astorga-Acuña

Biology Faculty Publications and Presentations

Animals may engage in coprophagy, including heterospecific coprophagy, where they consume the feces of other species. The white-tailed deer, Odocoileus virginianus, primarily feeds on plant material, such as tender forbs and shrub shoots. This report documents a case of heterospecific coprophagy by the white-tailed deer in Costa Rica, where two individuals were observed consuming domestic horse feces. The reasons for heterospecific coprophagy in ruminants like the white-tailed deer are unclear. Nutritional supplementation, microbial transfer, or other ecological factors may play a role in this behavior. However, such a behavior also poses risks, including the transmission of diseases and parasites from …

Wasting Disease Of A Marine Foundation Species Links Community Interactions To Disease Dynamics, Lillian R. Aoki, Olivia J. Graham, Reyn M. Yoshioka, Rebecca Maher, Lindsay Alma, Eileen E. Hofmann, Colleen A. Burge, Drew Harvell, Maya L. Groner

CCPO Publications

Infectious disease plays a key role in shaping marine communities, including in seagrass meadows, which form biodiverse coastal habitats. Eelgrass (Zostera marina) is the most widespread seagrass species and is susceptible to seagrass wasting disease, caused by the protist Labyrinthula zosterae. As a foundation species, eelgrass strongly influences ecosystem structure, function and services; recent work has begun to explore the links between critical community interactions and seagrass wasting disease. Here, we highlight recent advances about how the eelgrass community regulates and responds to seagrass wasting disease, from the microbiome to herbivores and filter feeders. We further show …

Spatial And Temporal Shifts In Bacterial Communities And Nutrient Removal In Two Springfield Wastewater Facilities, Rubina Sherchan

Graduate Theses/Dissertations

Bacterial communities play a central role in the biological nutrient removal and overall performance of wastewater treatment plants (WWTPs). However, very few is known about the spatial and seasonal dynamics of these communities. This study explores the spatial and seasonal variations in bacterial community composition across multiple treatment stages in two municipal WWTPs—Southwest (SWTP) and Northwest (NWTP)—in Springfield, Missouri. Using 16S rRNA gene amplicon sequencing, I analyzed 87 samples collected during summer and winter of 2024 from influent, sludge, aeration basins, clarifiers, and final effluent stages. I retrieved 16.4 million (summer) and 3.2 million (winter) sequences. Proteobacteria dominated both seasons …

A Comprehensive Parcel-Level Dataset On Farmland Assessment: Addressing Grid-Cell Data Bias Estimation, Wai Yan Siu, Man Li, Arthur J. Caplan

ODU Articles

Grid-cell data are increasingly used in research due to the growing availability and accessibility of remote sensing products. However, grid-cell data often fails to represent the actual decision-making unit, leading to biased estimates in socio-economic analysis. To this end, this paper presents a comprehensive parcel-level dataset for Salt Lake County, Utah, spanning from 2008 to 2018. This dataset combines detailed spatial and temporal data on land ownership, land use, and preferential farmland tax assessments under the Greenbelt program. Compiled from multiple geospatial sources, the dataset includes nearly 200,000 parcel-year observations, providing valuable insights into landowner decision-making and the impact of …

Clbbm And Clplt2 Function Redundantly During Both Male And Female Gametophytes Development In Watermelon, Qiyan Liu, Dongfen Han, Jinfan Chen, Jie Wang, Denghu Cheng, Xiner Chen, Jiao Jiang, Shujjuan Tian, Jiafa Wang, Man Liu, Haizheng Xiong, Li Yuan

Horticulture Faculty Publications and Presentations

As a prerequisite for sexual reproduction, gametophyte development is an interesting process involving cell proliferation, differentiation and specialization. Gametogenesis has been extensively explored in model plants, but the regulatory mechanism of gametophyte development largely remains unknown in Cucurbitaceae species. In present study, we have shown that watermelon ClBBM and ClPLT2, two AP2/ERF transcription factors, participated in both male and female gametophyte development. Clbbm and Clplt2 single mutants resembled wild-type phenotypes in both vegetative and reproductive development. But Clbbm/Clplt2 double mutant showed partial pollen abortion and bore less seeds comparing to WT. Our results indicated that the abnormal pollen grains were …

Identification Of Salt Tolerance And Stress Response In Us Department Of Agriculture Tomato Germplasm At The Seedling Stage, Ibtisam Alatawi, Haizheng Xiong, Kenani Chiwina, Hanan Alkabkabi, Qun Luo, Neelendra K. Joshi, Xiaolun Sun, Xuan Zhuang, Kai-Shi Ling, Ainong Shi

Horticulture Faculty Publications and Presentations

Soil salinity is a significant abiotic factor that impedes sustainable crop production in key agricultural regions worldwide. Saline cultivation adversely affects soil quality, whereas the use of saline water for irrigation disrupts the physiological and biochemical processes of plants. Continuous irrigation with high salt concentrations leads to a gradual buildup of soil salinity, thus hindering optimal plant growth and development. Consequently, there is a growing emphasis on breeding salinity-tolerant cultivars of various crops. In this study, 71 tomato accessions sourced from 20 countries and provided by the US Department of Agriculture were evaluated under controlled greenhouse conditions and subjected to …

Artificial Intelligence In Cancer-Related Malnutrition And Cachexia: A Transformative Tool In Clinical Nutrition, Salvatore Carbone

EVMS School of Health Professions Faculty Publications

[Introduction] Malnutrition and cachexia are common complications in cancer patients, and they negatively influence prognosis, treatment efficacy, and tolerability as well as quality of life [[1], [2], [3]]. Accurately identifying and effectively managing malnutrition and cachexia in this population remains a clinical challenge. Conventional validated screening tools may lack the sensitivity and specificity required for early detection and personalized intervention in diverse cancer types and treatment settings [4,5]. Over the last decade, the use of artificial intelligence (AI), including machine learning (ML) and deep learning (DL) strategies, has shown promising results in clinical nutrition, with the potential to revolutionize nutritional …

Comprehensive Investigation Of Proteoglycan Gene Expression In Breast Cancer: Discovery Of A Unique Proteoglycan Gene Signature Linked To The Malignant Phenotype, Simone Buraschi, Gabriel Pascal, Federico Liberatore, Renato V. Iozzo

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

Solid tumors present a formidable challenge in oncology, necessitating innovative approaches to improve therapeutic outcomes. Proteoglycans, multifaceted molecules within the tumor microenvironment, have garnered attention due to their diverse roles in cancer progression. Their unique ability to interact with specific membrane receptors, growth factors, and cytokines provides a promising avenue for the development of recombinant proteoglycan-based therapies that could enhance the precision and efficacy of cancer treatment. In this study, we performed a comprehensive analysis of the proteoglycan gene landscape in human breast carcinomas. Leveraging the available wealth of genomic and clinical data regarding gene expression in breast carcinoma and …

In-Person Versus Peer-Coordinated Virtual Health Coaching For Metabolic And Behavioral Outcomes In Women In Sober Living Facilities, Craig R. Witt

Dissertations

Background: Women in early recovery from substance use disorder often face metabolic health vulnerabilities, yet physical health behaviors are frequently under-emphasized in sober living settings. Feasible and accessible wellness strategies are needed to support both behavioral and metabolic health in this population.

Purpose: To compare the effects of professionally delivered in-person health coaching versus peer-coordinated virtual health coaching on metabolic outcomes and health-promoting behaviors among women in sober living facilities.

Methods: In this quasi-experimental study, 32 women enrolled in a 12-week group-based health coaching program focused on nutrition, physical activity, and stress management; 18 completed post-intervention assessments (in-person: n = …

Protein Ingestion And High-Intensity Resistance Training Decrease Respiratory Exchange Ratio Following Exercise In Females Across Various Body Compositions., Lynnsey Bowling

Dissertations

Metabolic dysfunction is associated with excess adipose tissue and interventions to elicit improvements to body composition could combat these health consequences. Acute nutritional and exercise strategies have the potential to shift metabolic processes toward those favoring fat oxidation. The purpose of this study was to investigate the effects of high-intensity resistance training (HIRT) and pre-exercise protein (PRO) ingestion on post-exercise respiratory exchange ratio (RER) in lean (LN) and overweight (OW) females. Twenty recreationally-active females (LN: n =10; OW: n = 10) completed two experimental sessions, consisting of HIRT and two acute nutritional interventions: PRO and placebo (PLA). RER was analyzed …

Astaxanthin Supplementation Does Not Significantly Alter Cognitive Performance Following Mental Fatigue In Females, Gavin A. Barker

Dissertations

Mental fatigue (MF) is a psychobiological state caused by prolonged periods of demanding cognitive activity, often characterized by impairments to cognitive performance. Astaxanthin (AX) is a naturally occurring antioxidant that can cross the blood-brain barrier and may preserve cognition when presented with a MF challenge. As yet, no studies have yet been conducted in an all-female cohort. This is the first study to investigate 4-weeks of AX supplementation on markers of cognitive performance (reaction time (RT), speed, and responses correct per second (RCS), visual analog scale of mental exertion (VAS-E)) following acute MF. Twenty-five recreationally active female subjects (AX: n …

The Impact Of University Engagement On Psychosocial Factors Among Division I Female Student-Athletes, Ashley A. Cook

Dissertations

Background: Female student-athletes navigate unique psychosocial challenges balancing academics and athletics, yet limited research has explored the impact of university engagement on their social and emotional well-being. This study examines the impact of university engagement on sense of social fit (SSF), nonspecific psychological distress (NPD), and loneliness among Division I female student-athletes.

Method: A cross-sectional survey was conducted among female student-athletes (n = 111) from eight sports at a mid-sized NCAA Division I university in the Southern U.S. Participants completed validated measures using the SSF scale, UCLA Loneliness scale, and Kessler-6 Psychological Distress scale. An exploratory factor analysis was performed …

Comparison Of Post-Exercise Lipid Oxidation Rates Between Lower And 1 Upper-Body Resistance Training In Well-Trained Females, Alyssa L. Parten

Dissertations

INTRODUCTION: Lipid oxidation (LOx) is a relevant physiological process linked to metabolic health. Despite growing interest in resistance training (RT) as a means to influence substrate utilization LOx responses in females remain poorly characterized, with no study examining whether lower- and upper body RT elicits different LOx patterns during the recovery period following exercise. This distinction may  be relevant because lower-body exercises involves greater fat-free mass (FFM) and energy expenditure,  whereas upper-body exercises recruit less FFM, potentially influencing metabolic effects.

PURPOSE: To  determine if LOx rates after exercise differ between lower- and upper-body RT in well-trained females.

METHODS: Twenty-two females …

Menstrual Cycle Phase Exerts No Influence On Running Economy Parameters In Recreational Female Runners, Savanna K. Knight

Dissertations

Hormonal fluctuations that occur throughout the menstrual cycle (MC) have been shown to affect substrate utilization and oxygen uptake when running. These MC induced changes could manifest as altered running economy (RE) when expressed as energy cost (Ec) and oxygen cost (O2c). Therefore, the current study aimed to determine if RE differs between MC phases, the early follicular phase (E-FP) and the mid-luteal phase (M-LP). Twelve eumenorrheic female recreational runners (ages 25 ± 4y; VO2peak 40.9±4.6 ml/kg/min) completed submaximal RE bouts (60%, 70%, 80% of VO2peak) during the E-FP and M-LP. During each bout, oxygen consumption (VO2), respiratory exchange ratio …

Validation Of Nutrition Focus Physical Assessment Via Telehealth, Ann Flickinger Ms, Rd, Ldn, Melissa Faura Rd, Ldn, Shae Duka Mph

Clinical Nutrition Service

No abstract provided.

Micro/Nanoplastics And Cancer: Focus On Gastrointestinal Malignancies - A Narrative Review, Sana Rabeeah, Priyata Dutta, Ahmad Mahdi, Alejandra Vargas, Edward C. Oldfield, David A. Johnson

Department of Medicine Faculty Publications

Micro- and nanoplastics (MNPs) are plastic particles smaller than 5 mm and 1 μm, respectively, and are emerging environmental pollutants with growing implications for human health. These particles stem from either 'primary sources', such as intentionally manufactured microbeads and industrial abrasives, or 'secondary sources', where larger plastic items break down into smaller fragments over time. Human exposure primarily occurs through ingestion and inhalation, with contaminated seafood and plastic-laden food packaging representing key routes of entry. Once ingested, MNPs can cross the intestinal barrier, accumulate in gastrointestinal (GI) tissues, and trigger biological responses. Mechanistic studies reveal that MNPs induce oxidative stress, …

Assessing Inter-Organizational Collaboration Within The Transboundary Network Governing The Conservation Of Southern Resident Killer Whales, Dane Pedersen, Owen Temby, Antonia Sohns, Evelyn Roozee, Gordon M. Hickey

School of Earth, Environmental, & Marine Sciences Faculty Publications

The Southern Resident Killer Whale (SRKW) is an endangered and charismatic species whose home range spans the Salish Sea, an ecosystem that extends across the Canada-US border. Conserving and recovering the SRKW represents a high-profile transboundary governance challenge that depends heavily on collaboration between a wide range of organizations. To better understand the factors affecting transboundary SRKW governance in the Salish Sea, this study applies a multi-dimensional trust, control, perceived risk framework to assess the inter-organizational architecture supporting collaboration. The analysis is based on key informant interviews (n=32) and surveys (n=35) conducted with policy actors working for different organizations involved …

Cellphone Separation Modulates The Effects Of Working Memory Load On Ex-Gaussian Parameters Of Choice Reaction Time, Michael Gazzanigo, Alexa Quesnel, Catalina Roldan, Xiao Yang

Psychology Faculty Publications

Cognitive effects of cellphone dependency among young adults have garnered increasing research attention. While cellphones have been identified as a distractor in daily tasks, related psychological processes remain unclear. As a potential mechanism underlying those effects of cellphones, excessive working memory (WM) load has not yet been well examined. Our study investigated the effects of the mental representation of cellphone separation on WM. Seventy-five participants (M(age) = 21.3 years; 55 females, 20 males) were assigned into three groups: the cued separation, natural separation, or control group, and completed a block of choice reaction time (CRT) task, and a dual-task block: …

They Forgot We Were Seeds: Critical Counter-Narratives Supporting Culturally Informed Community Seed Work, Florentina Rodriguez

Antioch University Dissertations & Theses

This Professional Innovation dissertation utilizes Critical Counter-Narrative both as research methodology to generate narrative reflections and as praxis for liberatory transformation. Through research vignettes adapted from the author’s experience with critical collaborative autoethnographic seed work, this study produced critical counter-narratives aimed at addressing inequities in small-scale seed systems. These counter-narratives were incorporated into curriculum materials synthesized as an educational toolkit for a professional research

sister-project: the new Culturally Informed Community Seed Pledge and Code of Ethics (CICSP/COE). This project seeks to address disparity and extractive practices; outline relational pathways for reciprocity and respecting communities of origin; and provide evaluation metrics …

Becoming The Donkey Derby’S ‘Karen’: A Humane Educator’S Autoethnographic Journey Toward Anti-Speciesist Communication, Jesika Keener

Antioch University Dissertations & Theses

Language not only carries oppression as an educative force, but research indicates that language is inherently speciesist and highly pervasive (Ethical Globe, 2021; Guevara Labaca, 2017; Hamlett, 2024; Leach et al., 2023). However, the ontological commitment to speciesism is historically foundational, which makes the total eradication of speciesist communication only viable through an unemerged hypothetical future society (Kahn, 2011). For the possibilities of such a world to come into being, pivotal steps must first be taken to critically engage with the communicated forms of speciesism in order to better understand it and actively resist its reproduction—with the aspiration for this …

Psychological Techniques For Athletes With Orthopedic Musculoskeletal Injuries: A Systematic Review, Nicole L. Williams

Antioch University Dissertations & Theses

Musculoskeletal injuries and recovery from them play a vital role in both the physical and psychological wellbeing of the public. Existing research has been conducted primarily within the athlete population with rehabilitation from these injuries having a profound impact on the physical and mental health. One of the most frequent psychological responses related to sports injuries is a trauma response, which has been shown to hinder the rehabilitation process and to increase the likelihood for reinjury upon return to play. Embedded within the many inputs to trauma responses to these injuries are psychological distress reactions, in particular fear connected to …

Resilience Of Food Sovereignty Organizations To The Covid-19 Pandemic, Rachel M. Brice

Antioch University Dissertations & Theses

This sequential explanatory mixed methods study explored the organizational resilience of food sovereignty organizations (FSOs) in the northeastern United States during the COVID-19 pandemic. Using a novel “open structural learning approach” that integrates structural contingency theory, open systems theory, and organizational learning theory, this research examined how FSOs’ structural attributes, resource flows, and organizational culture shaped their ability to navigate crisis. The study engaged FSO leaders through surveys (n = 13) and in-depth interviews (n = 7), supplemented by archival data analysis, to explore factors that strengthened or diminished FSO resilience. Findings revealed that FSOs navigated COVID-19 with multiple concurrent …

The Role Of Floodplain Complexity In Wood Turtle Habitat Use, Laura S. Deming

Antioch University Dissertations & Theses

Wood turtles occupy cold, low-gradient riverine habitat throughout the northeastern United State and southeastern Canada. Stream alterations, such as dams, channel straightening, and floodplain development have diminished the natural hydrologic and sedimentation regimes that result in complex channel and floodplain geomorphology. Studies throughout the species’ range have quantified many aspects of their habitat, but none have quantified components of geomorphology of wood turtle sites. The goal of this study was to explore the role of stream channel and floodplain geomorphology in wood turtle habitat use, with specific objectives to (1) quantify components of stream channel and floodplain geomorphic complexity, (2) …

Pick Up A Frog, Or Pet A Dog; Animals Are Our First Teachers, Allison Ratner

Antioch University Dissertations & Theses

This dissertation’s goal was an attempt to answer the question: What are the emotional, cognitive, and/or spiritual effects of children’s relationships/interactions with animals that potentially impact their development? By utilizing autoethnography, I analyzed instances from my own life where interacting with animals changed me permanently; my thinking, my knowing, my feeling, my judgement, my empathy, my attitude, and my ethics. With a standpoint rooted in the research of experts from the fields of psychology and education, I combined my personal knowledge gained over a lifetime, to grant merit to the profundity of children’s relationships with animals. With the analyzed data, …

Behavioral Response Of Wild Greater Caribbean Manatees (Trichechus Manatus Manatus) To Playbacks Of Conspecifics In Maya Beach, Belize, Phoebe Sonia Hodson

Antioch University Dissertations & Theses

The behavioral response of the wild Greater Caribbean manatee (Trichechus manatus manatus) to the acoustic vocalizations of their closely related subspecies, the Florida manatee (Trichechus manatus latirostris), was examined for the first time in Placencia Lagoon, Maya Beach, Belize. The main goal of this study was to determine how vocalizations from conspecifics influence the behavior of the wild Greater Caribbean manatee and how this behavioral change may give insight into the functionality of manatee vocalizations. Three call types (squeak, squeal and high squeak) and chewing sounds were played to varying size aggregations of manatees. Broad behavioral …

Distribution And Ecology Of The Cedar Keys Mole Skink (Plestiodon Egregius Insularis), Kevin M. Enge, Louis A. Somma, Paul E. Moler, Travis M. Thomas, Matthew T. Fedler, John M. Matter, Dennis C. Haney

Papers in Herpetology

We conducted surveys for the Cedar Keys Mole Skink, Plestiodon egregius insularis (Mount), during 1989– 1999 and 2015–2024, examined museum vouchers, and compiled personal observations from others to determine the status and distribution of this subspecies, which has the smallest geographic distribution of the five subspecies and is being considered for federal listing. We observed Cedar Keys Mole Skinks on Airstrip Island, Atsena Otie, Cedar Point, Deer Island, North Key, Scale Key, Seahorse Key, Snake Key, and possibly Dog Island. We have anecdotal reports of Mole Skinks on Way Key, which contains the most potential habitat and is the main …