Life Sciences Commons^™

Implementing Food Science-Based Instruction In Career Technical Education Courses, Jasmine D. Hendrix

Theses and Dissertations

Students are exposed to food concepts in agriculture-based career technical education (CTE) courses which provide a gateway for students to become more aware of food science career pathways. Professional development for Mississippi (MS) CTE teachers is needed to effectively implement food science-based instruction since there is not a food science curriculum framework adopted in MS. The objective of this study was to assess a food science professional development training for MS CTE teachers that would increase their self-perceived knowledge of food science, self-perceived ability to conduct food science skills, and their self-efficacy to implement food science-based instruction. Thirty-one teachers participated …

Genetic Characterization Of Antimicrobial Activities Of Endophytic Bacteria Burkholderia Strains Ms455 And Ms389, Jiayuan Jia

Theses and Dissertations

Strains MS455 and MS389, endophytic bacteria, were isolated from healthy soybean plant growing adjacent to a patch of plants affected by charcoal rot disease, caused by the fungal pathogen Macrophomina phaseolina. The complete genomes of both strains were sequenced and identified as Burkholderia species Strain MS455 exhibits broad-spectrum antifungal activities against economically important pathogens, including Aspergillus flavus. Random and site-specific mutations were employed in discovery of the genes that share high homology to the ocf gene cluster of Burkholderia contaminans strain MS14, which is responsible for production of the antifungal compound occidiofungin. RNA-seq analysis demonstrated ORF1, a …

Minimizing Off-Target Herbicide Movement Using Novel Application Technology, Hayden B. Quick

Theses and Dissertations

Drift is a point of contention with pesticide applications, causing the need to research application methods that provide consistent efficacy while minimizing off-target movement. Experiments were conducted to evaluate eight undiluted herbicides on invasive woody plants, Chinese tallow (Triadica sebifera) and Callery pear (Pyrus calleryana), when applied individual plant treatment (IPT) via hack-and-squirt. Applications of undiluted aminocyclopyrachlor or imazapyr at 1 ml per 7.6 cm of tree diameter at breast height (DBH) made in the spring provided superior control over other herbicides or application timings. CamelBak® hydration reservoirs were evaluated for storage durability with eight undiluted herbicides. A third study …

Relationship Of Winter Hair Coat Shedding Ability Of Purebred Angus Dams With Mineral Status, Apparent Forage Digestibility, Uterine Artery Hemodynamics, And Calf Growth And Development, Randy Hunter Burnett

Theses and Dissertations

Winter hair coat shedding ability in southern U.S. cattle production has previously been associated with differences in adjusted birth and weaning weights of calves from dams that shed by June or later (Gray et al., 2011; Cauble et al., 2019). This study was developed to research possible avenues of why these relationships exist with winter hair coat shedding ability. The primary objectives of the following experiments were to: 1) evaluate how mineral status relates to winter hair coat shedding ability, 2) evaluate how winter hair coat shedding ability relates to apparent digestibility of forage, 3) evaluate winter hair coat shedding …

Physiological Roles Of Selenoprotein H In Mice, Qingzhou Wang

Theses and Dissertations

Low-hierarchy selenoproteins are sensitive to selenium (Se) deficiency and are proposed to confer the protection of body Se against age- and development-related diseases. Selenoprotein H (SELENOH), a low-hierarchy selenoprotein, is greatly downregulated by both dietary Se deficiency and age. To explore physiological roles of SELENOH, Selenoh knockout mice were employed.

Segregation analyses demonstrated reduced frequencies of homozygotes and heterozygotes among the neonates of the breeding combinations of Selenoh^+/- males vs. Selenoh^+/- or Selenoh^-/- females, demonstrating essential roles of SELENOH in embryogenesis. Litter sizes from these two breeding groups were comparable with control, suggesting a role of SELENOH …

Dissection Of The Genetic Architecture Of Grain Quality In Rice, Shuai Liu

Theses and Dissertations

Rice is an important human staple food for over half of the world’s population. Amylose content (AC), gelatinization temperature (GT), grain protein content (GPC), percentage grain chalkiness (PGC), and mineral content are important parameters for evaluating rice quality, which attracts customers and breeders. Only limited genes or QTLs (OsAAP6, OsGluA2, OsASN1, Chalk5, OsHMA3, etc.) are reported regulating rice GPC, PGC, and mineral content due to the lack of genetic knowledge and molecular markers. To dissect the genetic architecture of rice grain quality regulation, genome wide association studies (GWAS) were performed using two populations …

Virtual Reality And Postural Control: The Virtual Moving Room Paradigm, Hannah R. Freeman

Theses and Dissertations

Virtual reality (VR) is becoming increasingly important in balance training. However, the influence of VR generated visual perturbation, through the novel virtual moving room paradigm, is unknown. Thirty healthy individuals had their static balance assessed on a BTrackS balance plate under eight different conditions: baseline eyes open and eyes closed with and without VR, unexpected toward and away moving VR perturbation, and expected toward and away moving VR perturbation. Multiple statistical analyses were conducted, and the results revealed significantly higher postural sway variables in the unexpected moving toward trials compared to the other moving room conditions; significantly higher postural sway …

Evaluation Of Alternate Wetting And Drying Irrigation Management In Rice, Richard Lee Atwill Ii

Theses and Dissertations

Water level declines in the Mississippi River Valley Alluvial Aquifer (MRVAA) are attributed largely to withdrawals for rice (Oryza sativa L.) irrigation. This study was performed to determine if alternative irrigation strategies for rice could reduce withdrawal from the MRVAA without having an adverse effect on grain yield, grain quality, control of barnyardgrass, and profitability. Research was conducted in Stoneville, MS and 19 on-farm locations across the Delta region of Mississippi from 2014 through 2017 to determine the irrigation threshold for alternate wetting and drying (AWD) rice irrigation, the effect of AWD management on barnyardgrass control, and effects of …

Systematics And Population Structure Of Amblyomma Maculatum Group Ticks And Rickettsia Parkeri, An Emerging Human Pathogen In Southern Arizona, Usa, Michelle E. J. Allerdice

Theses and Dissertations

The recent discovery of Amblyomma maculatum sensu lato (s. l.) ticks in southern Arizona has renewed discussions around species designations for members of the Amblyomma maculatum tick group. Amblyomma maculatum s. l. from Arizona appear to be morphologically intermediate between A. maculatum sensu stricto (s. s.) and A. triste s. s. At present there is no conclusive species designation for the ticks from Arizona. My research focused on analyzing the systematics of both A. maculatum s. l. and Rickettsia parkeri, a common bacterial pathogen transmitted by these ticks.

In the laboratory, A. maculatum s. l. from Arizona and A. maculatum …

Predation Efficiency Of Lionfish (Pterois Volitans And P. Miles) In Differing Levels Of Habitat Complexity, Abigail Ehlers Kimbrel

All HCAS Student Capstones, Theses, and Dissertations

The invasion of the Indo-Pacific lionfish (Pterois sp.) into the Atlantic and Caribbean has received attention around the globe. Venomous spines, over-sized fanlike pectoral fins, slow movement, and novel feeding strategies have allowed lionfish to become effective apex- predators. Recent research has determined that lionfish use inshore mangrove habitats as foraging grounds, which are also used as nursery habitats for juvenile fish, thus reducing recruitment of native fish populations. The purpose of this study was three-fold: (1) determine the predation efficiency of lionfish in differing mangrove habitat complexities, (2) examine the influence of temperature and behavior of prey commonly found …

Hydrodynamic Assessment Of Natural And Nature-Based Features For Escatawpa River And Grand Bay In The Northern Gulf Of Mexico, Peter Bacopoulos, Karim Alizad, Davina Passeri, Matthew Bilskie, Stephen Medeiros, Scott Hagen

Faculty Publications

This presentation showcases a hydrodynamic assessment of natural and nature-based features (NNBFs) for the Pascagoula River, the Escatawpa River and Grand Bay, located along the Mississippi coast of the northern Gulf of Mexico. Two separate NNBF projects are being considered to: (1) restore the historical footprint (ca. 1848) of Grand Batture Island for coastal protection purposes; and (2) reconnect the hydraulics between the Escatawpa River and Grand Bay for ecosystem services purposes. The intended coastal protection benefits of the first project include buffering agency to wave attack and attenuation of storm surge with the restored island. The intended ecosystem services …

Burning And Management Alternatives On Forage Accumulation And Floristic Composition Of A Native Pasture, I. Heringer, A. V. A. Jacques

IGC Proceedings (1977-2023)

The forage accumulation and floristic composition of native pasture, submitted to burning and alternative management practices, was evaluated during one year. The experimental design was a completely randomized, with five treatments and three replications. The forage accumulation was evaluated in a grazing exclusion cage, and the floristic composition was estimated with Botanal method. The systems without burning were more productive and, among these, the forage accumulation was similar between the one without burning and without mowing and the improve treatments, and the annual accumulation was greater than 9,000 Kg/ha of green forage dry matter. The species Paspalum notatum, P. …

Nitrogen Flow To Duodenum Of Steers Grazing On Orchardgrass And Meadowfescue Pastures, Masaaki Hanada, Y. Aibibula, Meiji Okamoto

IGC Proceedings (1977-2023)

This experiment was conducted to evaluate nitrogen flow to the duodenum of steers grazing on orchardgrass (Dactylis glomerata L., OG) or meadowfescue (Festuca elatior L., MF) pasture. Holstein steers fitted with cannulas in the rumen and duodenum were used. Nitrogen content and in vitro dry matter digestibility of herbage did not differ between OG and MF pastures. Herbage nitrogen intake of steers grazing on OG and MF pastures averaged 229 and 271 g/day, respectively. Nitrogen flows to the duodenum of steers grazing on orchardgrass and meadowfescue pastures averaged 187 and 195g/d, respectively. Microbial nitrogen flow to the duodenum …

The Effects Of Post-Grazing Sward Conditions On Herbage Accumulation In Winter And Spring, F. P. Hughes, P. N. P. Matthews, C. Matthew

IGC Proceedings (1977-2023)

Two on-farm experiments were conducted in the winter and spring of 1998 on a New Zealand seasonal production dairy farm to determine the effect of herbage mass present after grazing on subsequent pasture growth rates. Experiments involved a range of post-grazing levels of herbage mass (870, 1140, 1390, 1640, 1920 kg DM/ha in winter, and 1100, 1420, 1700 and 1910 kg DM/ha in spring) representing a range in cow intakes of 6.1-20.0 and 7.6-18.5 kg DM/cow/day in winter and spring, respectively. In both experiments there was a range of postgrazing herbage mass (1200-1600 kg DM/ha in winter and 1400-1800 kg …

The Effects Of Various Carbon Sources And The Presence Of Vitamin B-12 On The Production Of 1,3-Propanediol By L. Reuteri., Kayden Stevenson

Fall Student Research Symposium 2021

Dairy product preservation has evolved over thousands of years and has been a major benefit to humanity by creating stable, nutrient-dense food sources. Today the lactic acid bacteria Lactobacillus reuteri is important in preserving fermented dairy products through the production of the compound reuterin. Reuterin (1,3-propanediol) demonstrates antibiotic properties that prevent the fermentation of dairy products by undesirable bacteria. The purpose of this study is to determine if L. reuteri can produce 1,3-propanediol from a variety of carbon sources. Bacterial strains were incubated in the presence of a carbon source with vitamin B-12 or without B-12 for seven days with …

Distribution, Abundance, And Population Genetics Of The Alligator Snapping Turtle (Macrochelys Temminckii) In Mississippi, Luke Pearson

Dissertations

The southeastern United States is a freshwater turtle biodiversity hotspot, second only to southeastern Asia, and this region is inhabited by one of the largest freshwater turtles in the world: the alligator snapping turtle (Macrochelys temminckii). This species was heavily harvested from the 1960’s to the 1980’s for the turtle soup industry, apparently contributing to substantial, range-wide population declines. These declines, coupled with anthropogenic impacts on riverine and floodplain habitats, have led to M. temminckii being petitioned for federal listing under the U.S. Endangered Species Act; it is currently under review by the U.S. Fish and Wildlife Service …

Granulins In Norm And Neurodegenerative Pathologies, Anukool Bhopatkar

Dissertations

Granulins (GRNs) are small, cysteine-rich modules produced from the proteolytic cleavage of the precursor protein called progranulin (PGRN). GRNs are present in the form of seven tandem repeats within the precursor and are known to be produced in the extracellular and in lysosomal environments. In physiology, PGRN and GRNs plays pleiotropic roles such as neuronal growth and differentiation, immunomodulation, wound healing. Recent studies have implicated pathological role for PGRN in Alzheimer disease (AD) and frontotemporal dementia (FTD) but specific mechanism(s) remains unclear. However, potential interactions between GRNs and Ab42 and TDP-43 seem like a plausible underlying mechanism. Studies presented here …

Dicer Represses Antiviral Innate Immunity Pathways In Mouse Embryonic Stem Cells, Chandan Gurung

Dissertations

Recent studies have demonstrated that embryonic stem cells (ESCs) are deficient in expressing type I interferons (IFN), the cytokines that play key roles in antiviral responses. However, the underlying molecular mechanisms and biological implications of this finding are poorly understood. In this study, I used a synthetic RNA-based assay that can simultaneously assess multiple forms of antiviral responses in ESCs. Dicer is an enzyme essential for RNA interference (RNAi), which is used as a major antiviral mechanism in invertebrates but not clear in vertebrates. RNAi activity is detected in wild-type ESCs but is abolished in Dicer knockout ESCs (D−/−ESCs) as …

Discovering Virally Encoded Proteins That Block Type Iv Crispr Immune Systems, Andrew Williams

Fall Student Research Symposium 2021

Bacteria and the viruses that infect them have been at war from the beginnings of life until today. Due to selective pressure from viral infection, bacteria have evolved various biological defense systems, including CRISPR-Cas systems that use a genetic memory of previous viral encounters to protect against future invasions. However, recently it has been shown that viruses have evolved counter-strategies to evade CRISPR systems. Virally encoded proteins called anti-CRISPRs use a variety of mechanisms to block the activity of CRISPR immune systems in order to infect bacterial cells. The Jackson lab at USU recently showed that a Type IV-A CRISPR-Cas …

Characterization Of The Atpase Activity Of Casding, Christian Cahoon

Fall Student Research Symposium 2021

The battle between bacteria and phage has been ongoing for eons. This battle has generated the evolutionary pressure necessary for the development of microbial immune systems. Characterization of these systems has led to the discovery of molecular tools such CRISPR-Cas systems. This system uses a genetic memory of past viral infections coupled with associated proteins to form ribonucleoprotein complexes which seek out and destroy foreign genetic elements. These systems have been repurposed by scientists to create powerful gene editing tools such as Cas9. With such powerful molecular tools being discovered, we have pursued the characterization of a relatively unknown system, …

Characterizing The Mechanisms Of C. Elegans Prmt1 Temperature Dependence, Arianna Towne

Fall Student Research Symposium 2021

Over time, cellular enzymes evolve through amino acid mutations which allow them to remain functional at temperatures specific to the host organism. This activity may be partially or completely lost when enzymes are removed from their optimal temperature range, as is observed for the C. elegans protein arginine methyltransferase 1 (cPRMT1). This construct demonstrates maximum enzymatic activity at the C. elegans optimum of 20°C, but no activity at 37°C where activity for mammalian PRMT1 variants is observed. Given dysregulation of PRMT1 has been linked to various disease states, we are interested in exploiting the biophysical mechanisms of cPRMT1 temperature dependence …

Determining The Nucleic Acid Binding Affinities Of Crispr-Associated Ding (Casding), Matt Armbrust

Fall Student Research Symposium 2021

CRISPR-Cas systems are adaptive prokaryotic immune systems that enable host cells to defend against attack from foreign nucleic acids such as phage infections or plasmids. CRISPR-Cas systems are diverse and encompass 2 classes, 6 types, and 33 subtypes. The Type IV-A CRISPR-Cas system from Pseudomonas aeruginosa strain 83 is composed of five different genes (csf1, csf2, csf3, cas6, and dinG). Type IV-A systems are poorly understood, and currently there is little research detailing their biological and biochemical mechanism of immunity. CasDinG, an ancillary protein within the Type IV-A system, is required for an immune response in vivo. However, the role …

True Grit (1969) And Feminism: How It Succeeds And Why We Should Expect Better, Courtney Isom

Fall Student Research Symposium 2021

Hollywood Westerns are certainly not renowned for their contribution to feminist plots or characters, but True Grit (1969) is one of the more forward thinking movies with respect to women’s roles. However, the movie still portrays traditional power structures of patriarchy that undermine the female character’s autonomy. Looking at this film through the lens of both Critical Rhetoric and a Gender Diversity Perspective allows us to see the nuances of Mattie Ross' stand against oppressive traditional roles, while also examining the ways in which she is still dominated by discursive patriarchal practices. Celeste Condit’s gender diversity perspective encourages ditching traditional …

Halotolerant Rhizosphere Bacteria: Isolation Of Rhizosphere Bacteria From Native Utah Plant Ceanothus Velutinus, Alyssa Nielsen

Fall Student Research Symposium 2021

Increasing incidences of drought forced farmers to use the secondary, degraded water for irrigation. These water sources are rich in salt concentrations. This project has started with the hopes of finding bacteria from the rhizosphere of a native to Utah plant, Ceanothus velutinus, that helps the plant survive the saltier conditions of Utah. The rhizosphere, a layer of soil attached to the roots of a plant, contains microorganisms that may contribute to the plants' abiotic and biotic stress resistance. These microorganisms are known as Plant Growth Promoting Rhizobacteria (PGPR). The roots and rhizosphere samples were collected from Tony Grove in …

Identification And Isolation Of Halotolerant Endophytes In Ceanothus Velutinus May Lead To Plant Health In Saline Conditions, Katherine Hewitt

Fall Student Research Symposium 2021

Plant-microbe relations are integral to plant survival and crop productivity. Part of a plant’s biosphere are endophytes, microbes found between the cells of a plant. Endophytes can be beneficial to help a plant cope with abiotic stressors such as salinity. In Utah, our soils are high in salinity due to topography and climate. Climate change has increased soil salinity across the world, and water availability is becoming scarcer leading agriculture to use more saline sources to irrigate. Thus, salt is a concern for farmers. We aim to identify halotolerant endophytes from Ceanothus velutinus, Snowbrush which is native to the …

Understanding How Changes In Precipitation Intensity Will Affect Vegetation In The Western U.S., Cristina Chirvasa

Fall Student Research Symposium 2021

Precipitation events are becoming more intense as the atmosphere warms, but it remains unclear how precipitation intensification will affect plant growth in arid and semiarid ecosystems. There is conflicting evidence suggesting that larger precipitation events may either increase or decrease plant growth. Here, we report the growth responses of herbaceous and woody plants to experimental manipulations of precipitation intensity in a cold, semi-arid ecosystem in Utah, USA. In this experiment, precipitation was collected and redeposited as fewer, larger events with total annual precipitation kept constant across treatments. Results from the first two growing seasons revealed that more intense events ‘pushed’ …

Log Proximity And Moss As Indicators Of Conifer Seedling Abundance In Old-Growth Douglas-Fir/ Hemlock Forests, Isabella Wetzler

Fall Student Research Symposium 2021

It is increasingly essential to develop a comprehensive understanding of forest processes so that we may better conserve and maintain our old-growth forests in the face of climate change. In the Pacific Northwest, logs might elevate and remove conifer seedlings from light competition with moss and herbs, but seedlings growing on the forest floor may benefit from log shade. Moss can retain moisture which may result in higher water availability for seedlings, but in droughty conditions the desiccated moss might leave seedlings ‘high and dry’. Logs increase mircrosite heterogeneity, providing cool, moist microclimates for seedlings and protecting them from droughty …

Climate Change Impacts On Atmospheric Ammonia And Implications For Human Health, Casey Olson, Connor Snow, Bridger Jorgensen

Fall Student Research Symposium 2021

According to national data Cache Valley has the highest concentrations of atmospheric ammonia in the nation. This study aims to answer the questions of whether climate variables and events such as precipitation, averaged winds, geopotential height, and teleconnections can be used to predict the behavior of pollutants and how human biology is potentially affected. Data from the Utah Climate Center shows that the 3rd yearly quartile has the highest levels of airborne ammonia due to the high levels of fertilizer use and livestock emissions in the farming industry in Cache Valley. After data analysis, there seems to be a connection …

A Method To Assess Response Inhibition During A Balance Recovery Step, Molly Rowley

Fall Student Research Symposium 2021

Background: Correlations between falls and executive function tests, particularly those tests emphasizing inhibitory control, suggests that the ability to suppress automatic, but unwanted action, is important in fall prevention. Response inhibition has been a topic of considerable interest in the cognitive neuroscience community for many decades, bringing with it, the development of techniques that could be used to inform assessment of reactive balance. Research question: Can we apply a method used in traditional cognitive testing - the stop signal task - to measure response inhibition in a speeded, balance recovery task? Methods: Twenty healthy, young adults completed a novel reactive …

Synthetic Hagfish Slime Mimetics: Mechanical Characterization, Hayden Johns, Spencer Walker

Fall Student Research Symposium 2021

Hagfish are ancient animals that eject a slime when attacked by predators. The slime that the hagfish emit is almost entirely composed of water but protein strands within the slime cause the slime to have incredible strength. However, these protein strands will be formed synthetically for the purposes of our experiment. To defend against foes, the Navy launches plastic ropes into the propellers of enemy warships in order to decrease the thrust of the motors. In a push to find a more biodegradable solution, the utilization of hagfish slime has shown great promise in stopping propellers.While we know that the …