Life Sciences Commons^™

Records Of Hippoboscidae From Mongolian Birds Of Prey With Checklist, Update, Matthias Jentzsch, Benjamin Meissner, Nayamsuren Batsaikhan, Annegret Stubbe, Michael Stubbe

Erforschung biologischer Ressourcen der Mongolei / Exploration into the Biological Resources of Mongolia, ISSN 0440-1298

The hippoboscid species Ornithophila gestroi has been rediscovered from Mongolia. It is the easternmost of all the known occurrences. Specimens were collected from Amur Falcon Falco amurensis, Saker Falcon Falco cherrug, Lesser Kestrel Falco naumanni, Eurasian Hobby, Falco subbuteo, Common Kestrel Falco tinnunculus Linnaeus, 1758 and Black Kite Milvus migrans in a breeding area located in southern Mongolia. Amur Falcon, Eurasian Hobby and Black Kite were identified as hosts of O. gestroi for the first time.

High Prevalence Of Pre-Existing Serological Cross-Reactivity Against Severe Acute Respiratory Syndrome Coronavirus-2 (Sars- Cov-2) In Sub-Saharan Africa, For Yue Tso, Salum Lidenge, Phoebe B. Peña, Ashley A. Clegg, John R. Ngowi, Julius Mwaiselage, Owen Ngalamika, Peter Julius, John T. West, Charles Wood

Nebraska Center for Virology: Faculty Publications

Objective: Significant morbidity and mortality have occurred in the USA, Europe, and Asia due to severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), whereas the numbers of infections and deaths in sub-Saharan Africa (SSA) have remained comparatively low. It has been hypothesized that exposure of the population in SSA to other coronaviruses prior to the COVID-19 pandemic resulted in some degree of cross-protection against SARS-CoV-2 infection and pathogenesis. We evaluated this hypothesis by comparing SARS-CoV-2 cross-reactive antibodies in pre-pandemic plasma samples collected from SSA and the USA.

Method: Pre-COVID-19 pandemic plasma samples from SSA and the USA were collected and tested by …

The 20th Anniversary Meeting Of The Rocky Mountain Virology Association, Joel Rovnak, Laura A. St Clair, Carley Mcalister, Chinemerem P. Ogbu, Jessica Smolenske, Randall J. Cohrs, Rushika Perera

Nebraska Center for Virology: Faculty Publications

Due to the COVID-19 pandemic and multiple devastating forest fires, the 2020 meeting of the Rocky Mountain Virology Association was held virtually. The three-day gathering featured talks describing recent advances in virology and prion research. The keynote presentation described the measles virus paradox of immune suppression and life-long immunity. Special invited speakers presented information concerning visualizing antiviral effector cell biology in mucosal tissues, uncovering the T-cell tropism of Epstein-Barr virus type 2, a history and current survey of coronavirus spike proteins, a summary of Zika virus vaccination and immunity, the innate immune response to flavivirus infections, a discussion concerning prion …

Chlorovirus Pbcv-1 Multidomain Protein A111/114r Has Three Glycosyltransferase Functions Involved In The Synthesis Of Atypical N-Glycans, Eric Noel, Anna Notaro, Immacolata Speciale, Garry A. Duncan, Cristina De Castro, James L. Van Etten

Nebraska Center for Virology: Faculty Publications

The structures of the four N-linked glycans from the prototype chlorovirus PBCV-1 major capsid protein do not resemble any other glycans in the three domains of life. All known chloroviruses and antigenic variants (or mutants) share a unique conserved central glycan core consisting of five sugars, except for antigenic mutant virus P1L6, which has four of the five sugars. A combination of ge- netic and structural analyses indicates that the protein coded by PBCV-1 gene a111/114r, conserved in all chloroviruses, is a glycosyltransferase with three putative domains of approximately 300 amino acids each. Here, in addition to in silico sequence …

Strategies Targeting Hemagglutinin As A Universal Influenza Vaccine, Brianna L. Bullard, Eric A. Weaver

Nebraska Center for Virology: Faculty Publications

Influenza virus has significant viral diversity, both through antigenic drift and shift, which makes development of a vaccine challenging. Current influenza vaccines are updated yearly to in- clude strains predicted to circulate in the upcoming influenza season, however this can lead to a mismatch which reduces vaccine efficacy. Several strategies targeting the most abundant and im- munogenic surface protein of influenza, the hemagglutinin (HA) protein, have been explored. These strategies include stalk-directed, consensus-based, and computationally derived HA immunogens. In this review, we explore vaccine strategies which utilize novel antigen design of the HA protein to improve cross-reactive immunity for development …

Zika Virus Infection Induces Endoplasmic Reticulum Stress And Apoptosis In Placental Trophoblasts, Philma Glora Muthuraj, Prakask K. Sahoo, Madison Kraus, Taylor Bruett, Arun S. Annamalai, Aryamav Pattnaik, Asit K. Pattnaik, Siddappa N. Byrareddy, Sathish Kumar Natarajan

Nebraska Center for Virology: Faculty Publications

Zika virus (ZIKV) infection to a pregnant woman can be vertically transmitted to the fetus via the placenta leading to Congenital Zika syndrome. This is characterized by microcephaly, retinal defects, and intrauterine growth retardation. ZIKV induces placental trophoblast apoptosis leading to severe abnormalities in the growth and development of the fetus. However, the molecular mechanism behind ZIKV-induced apoptosis in placental trophoblasts remains unclear. We hypothesize that ZIKV infection induces endoplasmic reticulum (ER) stress in the trophoblasts, and sustained ER stress results in apoptosis. HTR-8 (HTR-8/SVneo), a human normal immortalized trophoblast cell and human choriocarcinoma-derived cell lines (JEG-3 and JAR) were …

Molecular Identification Of Parasites In An Intestinal Coprolite From A Mummified Religious Dignitary Of The Piraino Mother Church Crypt, Sicily, Amanda Rollins, Krystiana Krupa, Georgia Millward, Dario Piombino-Mascali, Karl Reinhard, Frederika Kaestle

Karl Reinhard Publications

Intestinal contents were sampled from a spontaneously enhanced mummy from the Sepulcher of the Priests of the Piraino Mother Church in the Province of Messina, Sicily. This adult male mummy, Piraino 1, is an unidentified religious dignitary dating from the late-18th to mid-19th centuries. Immunological and molecular diagnostics were used to test for common and clinically significant parasites. A morphological diagnosis of Trichuris trichiura (whipworm) was confirmed genetically. A previously undetected Enterobius vermicularis (pinworm) infection was also identified genetically. These data indicate that the Piraino 1 individual was simultaneously infected with multiple intestinal parasite species indicative of poor hygiene. This …

Biodiversity Of The Coccidia (Apicomplexa: Conoidasida) In Vertebrates: What We Know, What We Do Not Know, And What Needs To Be Done, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Over the last two decades my colleagues and I have assembled the literature on a good percentage of most of the coccidians (Conoidasida) known, to date, to parasitise: Amphibia, four major lineages of Reptilia (Amphisbaenia, Chelonia, Crocodylia, Serpentes), and seven major orders in the Mammalia (Carnivora, Chiroptera, Lagomorpha, Insectivora, Marsupialia, Primates, Scandentia). These vertebrates, combined, comprise about 15,225 species; only about 899 (5.8%) of them have been surveyed for coccidia and 1,946 apicomplexan valid species names or other forms are recorded in the literature. Based on these compilations and other factors, I extrapolated that there yet may be an additional …

Differential Effects Of Whole Red Raspberry Polyphenols And Their Gut Metabolite Urolithin A On Neuroinflammation In Bv-2 Microglia, Ashley Mulcahy Toney, Mahaa Albusharif, Duncan Works, Luke Polenz, Stacie Schlange, Virginia Chaidez, Amanda E. Ramer-Tait, Soonkyu Chung

Department of Nutrition and Health Sciences: Faculty Publications

Whole red raspberry polyphenols (RRW), including ellagic acid, and their gut-derived metabolite, urolithin A (UroA), attenuate inflammation and confer health benefits. Although results from recent studies indicate that polyphenols and UroA also provide neuroprotective effects, these compounds differ in their bioavailability and may, therefore, have unique effects on limiting neuroinflammation. Accordingly, we aimed to compare the neuroprotective effects of RRW and UroA on BV-2 microglia under both 3 h and 12 and 24 h inflammatory conditions. In inflammation induced by lipopolysaccharide (LPS) and ATP stimulation after 3 h, RRW and UroA suppressed pro- inflammatory cytokine gene expression and regulated the …

Covid-19 Contagion Among Communities With Limited English Proficiency: Lesson From Volunteerism, Falah N. Rashoka M.S

Department of Nutrition and Health Sciences: Faculty Publications

The COVID-19 affects all communities in the United States, but its impact differs from one community to another. Undoubtedly, the vulnerable populations are more impacted than others, especially refugees and immigrants. This study seeks to understand the impact of COVID-19 on marginalized communities and the need to communicate health-related information equally. A lesson learned from observation during an ongoing pandemic, including what I learned through my volunteer work between mid-March and August 2020. The study finds that neighbors with limited English Proficiency (LEP) and limited education could quickly transfer the COVID-19 virus to another neighbor with high income and well-educated. …

The Effects Of Phosphocreatine Disodium Salts Plus Blueberry Extract Supplementation On Muscular Strength, Power, And Endurance, John Paul V. Anders, Tyler J. Neltner, Robert W. Smith, Joshua L. Keller, Terry J. Housh, F Joseph Daugherty, Michael S. Tempesta, Alekha K. Dash, Daniel J. Munt, Richard J. Schmidt, Glen O. Johnson

Department of Nutrition and Health Sciences: Faculty Publications

Background: Numerous studies have demonstrated the efficacy of creatine supplementation for improvements in exercise performance. Few studies, however, have examined the effects of phosphocreatine supplementation on exercise performance. Furthermore, while polyphenols have antioxidant and anti-inflammatory properties, little is known regarding the influence of polyphenol supplementation on muscular strength, power, and endurance. Thus, the purpose of the present study was to compare the effects of 28 days of supplementation with phosphocreatine disodium salts plus blueberry extract (PCDSB), creatine monohydrate (CM), and placebo on measures of muscular strength, power, and endurance.

Methods: Thirty-three men were randomly assigned to consume either PCDSB, CM, …

Essential Role Of Systemic Iron Mobilization And Redistribution For Adaptive Thermogenesis Through Hif2-Α/Hepcidin Axis, Jin-Seon Yook, Mikyoung You, Jiyoung Kim, University Of Nebraska-Lincoln, Rong Fan, Bhanwar Lal Puniya, Tomáš Helikar, Sophie Vaulont, Jean-Christophe Deschemin, Meshail Oklar, Liwei Xie, Manik C. Ghosh, Tracey A. Rouault, Jaekwon Lee, Soonkyu Chung

Department of Nutrition and Health Sciences: Faculty Publications

Iron is an essential biometal, but is toxic if it exists in excess. Therefore, iron content is tightly regulated at cellular and systemic levels to meet metabolic demands but to avoid toxicity. We have recently reported that adaptive thermogenesis, a critical metabolic pathway to maintain whole-body energy homeostasis, is an irondemanding process for rapid biogenesis of mitochondria. However, little information is available on iron mobilization from storage sites to thermogenic fat. This study aimed to determine the iron-regulatory network that underlies beige adipogenesis. We hypothesized that thermogenic stimulus initiates the signaling interplay between adipocyte iron demands and systemic iron liberation, …

A Study On The Pathological Effects Of Trypanorhyncha Cestodes In Dusky Groupers Epinephelus Marginatus From The Canary Islands, Carolina De Sales-Ribeiro, Miguel A. Rivero, Antonio Fernández, Natalia García-Álvarez, Jorge Francisco González, Oscar Quesada-Canales, María José Caballero

Harold W. Manter Laboratory of Parasitology: Library Materials

Trypanorhyncha are cestodes commonly infecting marine fish. Numerous studies have detailed the biology of Trypanorhyncha species, but information on the pathological changes produced by these parasites is limited. Dusky groupers are keystone species necessary for the preservation of several marine ecosystems. Considering their vulnerable state of conservation and the efforts being made to culture them, identification of the effects caused by Trypanorhyncha is vital. Here, we aimed to determine the prevalence and pathological changes produced by Trypanorhyncha in dusky groupers from the Canary Islands. The prevalence of trypanorhynch plerocerci was 96%. Grossly, in the abdominal cavity, there were numerous larvae-filled …

Jaguars And Pumas Exhibit Distinct Spatiotemporal Responses To Human Disturbances In Colombia’S Most Imperiled Ecoregion, Joe J. Figel, Sebastián Botero-Cañola, Juan David Sánchez-Londoño, Javier Racero-Casarrubia

University of Nebraska State Museum: Mammalogy Papers

Abstract

Coexistence of sympatric felids is facilitated by mutual avoidance and the partitioning of habitats, prey, and time. Anthropogenic disturbances disrupt this coexistence in fragmented landscapes, potentially triggering cascading influences in ecological communities. We used photographic data from 8,717 trap nights (November 2014–June 2016) at 87 camera trap sites in Colombia’s middle Magdalena River basin to compare spatiotemporal overlap among jaguars (Panthera onca), pumas (Puma concolor), their prey, and humans, at sites of high and low disturbance, as determined by the human influence index. Human disturbance events (e.g. domestic dogs, livestock, and humans, including armed hunters) …

Phospholipid R Ecovery From Sweet Whey And Whey Protein Concentrate: Use Of Electrodialysis With Bipolar Membrane Combined With A Dilution Factor As An Ecoefficient Method, Mélanie Faucher, Véronique Perreault, Ozan Nazim Ciftci, Sami Gaaloul, Laurent Bazinet

Department of Food Science and Technology: Faculty Publications

Electrodialysis with bipolar membrane (EDBM) is promising to recover phospholipids (PLs) from sweet whey and whey protein concentrate (WPC), as it promotes lipoprotein complex formation following a decrease in pH and ionic strength. The aim of this work was to study the impact of dilution factor (without dilution, with a 2X, 4X and 6X dilution) after EDBM on the process performances. For both products, a 4X dilution, which cor- responds to a decrease in ionic strength of 81.4 ± 1.5 % for sweet whey and 79.4 ± 0.4 % for WPC, seemed sufficient to maximize lipoprotein complex formation as a …

Patch Utilization And Flower Visitations By Wild Bees In A Honey Bee-Dominated, Grassland Landscape, Clint R. V. Otto, Larissa L. Bailey, Autumn H. Smart

Department of Entomology: Faculty Publications

Understanding habitat needs and patch utilization of wild and managed bees has been identified as a national research priority in the United States. We used occupancy models to investigate patterns of bee use across 1030 transects spanning a gradient of floral resource abundance and richness and distance from apiaries in the Prairie Pothole Region (PPR) of the United States. Estimates of transect use by honey bees were nearly 1.0 during our 3.5-month sampling period, suggesting honey bees were nearly ubiquitous across transects. Wild bees more frequently used transects with higher flower richness and more abundant flowers; however, the effect size …

Analysis Of Genetic Variation In An Important Pest, Tuta Absoluta, And Its Microbiota Witha New Bacterial Endosymbiont, Fariba Mehrkhou, Nurper Güz, Ertan Mahi̇r Korkmaz, Naci̇ye Sena Çağatay

Turkish Journal of Agriculture and Forestry

Tomato leaf miner is an aggressive and invasive pest that causes serious damage in tomato production, resulting in up to 100% yield losses during serious outbreaks. The management of tomato leaf miner with synthetic pesticides is difficult due to its leaf mining habit, short life span, high reproductive rates, and insecticide resistance problem. In this study, sequences of the mitochondrial cytochrome oxidase I (mtCOI) gene of Tuta absoluta specimens were analyzed and compared with the available COI sequence data members from GenBank. All the specimens of tomato leaf miner were clustered together in a single group with a high support …

Investigation Of Some Enthomopathogens As Biocontrol Agents Of Tinocallis (Sappocallis) Saltans (Nevsky, 1929) (Hemiptera: Aphididae), Eli̇f Tozlu, Göksel Tozlu, Recep Kotan, Önder Çalmaşur, Fati̇h Dadaşoğlu

Turkish Journal of Agriculture and Forestry

In Turkey, Tinocallis (Sappocallis) saltans (Nevsky) on Ulmus glabra Hudson was detected for the first time, and it was determined that it caused a considerable amount of damage. The study was conducted for the purpose of determining the usability of enthomopathogens in the control against it, while avoiding the negative effects of chemicals on the environment and human health. A total of 12 bacteria strains were tested for control efficacy and verified: Brevibacillus brevis (2), Bacillus thuringiensis (1), Bacillus thuringiensis subsp. kenyae (2), Bacillus thuringiensis subsp. kurstakii (2), Bacillus subtilis (1), Pseudomonas chlororaphis (1), Bacillus sphaericus GC subgroup D (1), …

Co2 Respiration Rates Of Einkorn Wheat At Different Temperature And Moisture Contents, Hakan Ki̇bar

Turkish Journal of Agriculture and Forestry

Temperature and grain moisture content are the main factors affecting grain spoilage in storage. These factors have a significant impact on the maintenance of quality, insect, fungus, and pest development. The main objective of this study was to determine the CO2 concentration and respiration rate of hulled einkorn wheat in different temperatures (5, 10, 15, 20, 25, 30, and 35 °C) and grain moisture contents (9.1%, 11.5%, 13.8%, and 15.9%, wet basis) by means of CO2 sensors. Multiple polynomial regression equation was developed to predict respiration as affected by temperature and grain moisture content. The CO2 concentrations and respiration rates …

Effects Of Position Angles In Twin-Jet Spray Applications On Droplet Penetration Of Hydraulic Nozzles, Bahadir Sayinci, Bünyami̇n Demi̇r, Emel Çeti̇n

Turkish Journal of Agriculture and Forestry

This study was conducted to determine the effects of different position angles in twin-jet spray applications on droplet penetration of different nozzle types. Seven different nozzle types (standard flat fan, ST; narrow flat fan, STN; multirange, LU; low-drift potential, AD; air-induction, IDK; twin-jet air-induction, IDKT) were used and nozzles were positioned +15° along the forward direction, perpendicular to ground surface 0? and reverse direction of forward -15°. Spray experiments at 100 L/ha constant application volume were conducted under controlled conditions of a closed facility. Water sensitive paper (WSP) was used as sampling surface. WSP samples were placed vertically and horizontally …

Impact Of Roasting Level On Fatty Acid Composition, Oil And Polycyclic Aromatic Hydrocarbon Contents Of Various Dried Nuts, Emel Öz, Eli̇f Eki̇z, Adem Savaş, Eyad Aoudeh, A.M. Abd El-Aty, Fati̇h Öz

Turkish Journal of Agriculture and Forestry

Herein, the effects of roasting levels (rare, medium, and well) on the fatty acid composition, oil and polycyclic aromatic hydrocarbon (PAH) contents of various dried nuts (almonds, hazelnuts, peanuts, and cashews) were investigated. The roasting level affected the moisture and total oil contents, as well as the fatty acid composition. The roasting level had no significant effect (P > 0.05) on saturated fatty acid (SFA), monounsaturated fatty acid (MUFA) contents of the dried nuts, while it had a significant effect (P < 0.05) on polyunsaturated fatty acid (PUFA). In raw and dry roasted nut, the most common SFA, MUFA, and PUFA were palmitic, oleic, and linoleic acid, respectively. The amounts of linoleic acid and ΣPUFA, as well as ΣPUFA/ΣSFA ratio were the lowest in the well roasted samples. Additionally, the types of dried nut had a significant effect (P < 0.01) on the amounts of total oil and fatty acid compositions. The highest total oil amount (62.15%) was found in hazelnuts, while the lowest PUFA content (7.54%) was also determined in hazelnuts. From the nutritional perspective of view, the highest ΣPUFA / ΣSFA ratio (2.40) belonged to almonds. Although the recovery rates of PAH standards varied between 70.14%-85.10%, none of the heavy PAH compounds could be detected in raw and roasted samples.

Biogenic Amines Contents Of Van Herby Cheese Matured By Different Methods, Rumeysa Bozkurt, İbrahi̇m Altun

Turkish Journal of Agriculture and Forestry

In this study, biogenic amines levels of Van herby cheese (Van Otlu peynir) matured in dry-salted/filling (basma) and brine (salamura)-salted techniques were investigated. For this purpose, a total of 100 herby cheese samples matured by dry-salted and brine-salted techniques (50 dry-salted and 50 brine-salted) were purchased from the grocery stores in Van province, and chemical characteristics and biogenic amines contents of the samples were determined. The mean chemical composition for Van herby cheese matured by dry-salted method and brine-salted methods were, respectively, as follows: pH value 4.62 and 5.16, acidity 1.50% and 1.08%, salt content 7.85% and 7.15%, dry matter …

Reforming Cotton Genes: From Elucidation Of Dna Structure To Genome Editing, Muhammad Salman Mubarik, Sajid Majeed, Sultan Habibullah Khan, Xiongming Du, James E. Frelichowski, Lori Hinze, Muhammad Tehseen Azhar

Turkish Journal of Agriculture and Forestry

Cotton is an essential fiber producing crop in the world. It also supports additional industries by providing high quality oil and protein in the form of cottonseed cake. Currently, there is an urgent need to increase lint yield, fiber quality, and resistance to biotic and abiotic stresses due to rising pressure from a global population and possible supply shortages from the effects of erratic climate changes. Classic plant breeding and transgenic strategies need more genetic breakthroughs to support the increasing pressure for fiber quantity and quality. A potential for rapid increases in crop improvement is in various state-of-the-art gene editing …

A Smart Agricultural Application: Automated Detection Of Diseases In Vine Leaves Usinghybrid Deep Learning, Ahmet Alkan, Muhammed Usame Abdullah, Hanadi̇ Omai̇sh Abdullah, Muhammed Assaf, Huiyu Zhou

Turkish Journal of Agriculture and Forestry

This paper reports a study which utilizes deep learning for automated detection of the symptoms of diseases on vine leaves. Vine fruits or grapes are very important and have existed in Syria and surrounding areas (e.g., Turkey) for many years. Quality of vine fruits is also very important in grape production as it is consumed in these areas every day. The aim of this study is to improve diseasedetection accuracy in vine leaves and to develop a system to help Syrian and Turkish farmers and agricultural engineers to maintain the quality of grape production. In this study, over 1000 images …

Analysis Of Designs Used In Monitoring Crop Growth Based On Remote Sensing Methods, Cristina Teodora Dobrota, Rahela Carpa, Anca Butiuc-Keul

Turkish Journal of Agriculture and Forestry

Choosing appropriate designs and methods for monitoring crop growth is a challenging process of major importance. Remote sensing from space and manned or unmanned airborne operations are used to measure crop reflectance and a wide variety of other agricultural parameters. While some experiments use only a few, specific methods and designs and organize the results in lists of evidence, other experiments use a wider range of techniques to create a more credible and comprehensive assessment of crop yield. Particular situations related to the available resources in terms of data collection and expertise in addition to the intended use of the …

Fine Root Vertical-Seasonal Distribution Of Robinia Pseudoacacia In Relation To Abioticfactors In A Chronosequence In Coastal Saline Alkali Land Of The Yellow River Delta, China, Longmei Guo, Banghua Cao, Peili Mao, Zexiu Li, Muzheng Hao, Tiantian Wang, Fucheng Jiang

Turkish Journal of Agriculture and Forestry

Despite the recognized importance of the fine roots in influencing tree growth and stand productivity, knowledge of fine roots is still limited, especially in adverse sites like saline-alkali land. In this paper, we applied sequential soil coring to assess the influence of stand age, soil depth and growth month on fine roots in 3, 16, 30 and 40-year old Robinia pseudoacacia stand in coastal saline-alkali land of the Yellow River Delta, China. Besides, the correlations between fine roots characteristics fine root biomass (FRB), fine root length (FRL), fine root surface area (FRSA), specific root length (SRL) and specific root surface …

Impacts Of Gümüşhane Cement Dust Emissions On Soil Elemental Compositions, Serdar Bi̇len, Müdahi̇r Özgül, Ekrem Özlü, Murat Bi̇len

Turkish Journal of Agriculture and Forestry

The cement dust deposition can cause environmental pollution and heavy metal contamination, which negatively impacts soil nutrient availability and hence crop productivity. Thus, this study evaluates the impact of cement dust emissions on soil elemental compositions in different tillage managements. In this study, composite soil samples were taken from conventional tillage (CT), and notill (NT) managed fields under wheat-sugar beet (potato)-fallow cropping sequence. Soil samples were randomly collected from 0-30 cm depth in three replications and different distances (1, 2, 4, 6, 8, and 10 km) from a cement plant. Soil pH, clay, and CaCO3 contents were higher under CT …

Apple Leaf Disease Detection And Classification Based On Transfer Learning, Cevher Özden

Turkish Journal of Agriculture and Forestry

The world population and the number of people affected by hunger constantly increases. Precision farming offers new solutions to a modern and more fertile production in agriculture. Early and in-place disease detection is one of the fields that recent studies have focused on. The present paper introduces a new approach to transfer learning in that training, validating and testing of the model have been made on images from different sources to see its effectiveness. Several optimization methods including the adaptation of a recent custom PowerSign optimization algorithm are compared in the study. Accordingly, the model with Adagrad optimizer produced more …

Genetic Characterization Of Almond (Prunus Amygdalus L) Using Microsatellite Markersin The Area Of Adriatic Sea, Jasna Hasanbegovic, Semina Hadziabulic, Mirsad Kurtovic, Fuad Gasi, Biljana Lazovic, Boris Dorbic, Azra Skender

Turkish Journal of Agriculture and Forestry

The use of microsatellite (SSR) markers has successfully found its application in genetic characterization and examination of the origin of a large number of fruit species. Mediterranean germplasm is characterized by a great variety of almond genotypes. The study covered two geographically distant regions Montenegro (Bar) and Croatia (Sibenik) in a sample of 60 almond genotypes. Genetic analysis of almonds involved the use of ten microsatellite primers for genetic characterization of 60 examined genotypes, which successfully amplified PCR products and were highly polymorphic. Nine microsatellite markers used for the genetic characterization of almonds are derived from Prunus persica (UDP97-402, UDP98-411, …

Morphological And Molecular Characterization Of Croatian Carob Tree(Ceratonia Siliqua L.) Germplasm, Snjezana Bolaric, Ivna Dragojevic Müller, Ales Vokurka, Dubravka Vitali Cepo, Mirko Ruscic, Sinisa Srecec, Dario Kremer

Turkish Journal of Agriculture and Forestry

The results of morphological and AFLP variability of 120 plants of carob tree (Ceratonia siliqua L.), collected from 12 different locations (10 biological replicates for each location) on the coast and islands of the southern Croatian Adriatic, indicate high molecular and morphological variability among these carob populations. Analysis of molecular variance revealed significant differences among populations (26.07%; p < 0.001; a = 0.05). Out of the total variability, 22.49% refers to the variability among, and 77.51% within populations. UPGMA and STRUCTURE analysis based on AFLP genetic data clustered carob populations into three main groups representing three real genetic populations. UPGMA analysis based on morphological traits of leaves, pods, and seeds clustered carob populations into five groups. Mantel test showed significant correlation between morphological and genetic data (r = 0.58, p < 0.001; a = 0.05). According to the high genetic and morphological variability, the germplasm collection in the analysis could represent an important genetic pool for future breeding programmes. The goal of future research should be the conservation of C. siliqua in its natural habitats, and the establishment of gene banks of genetic resources with the purpose of creating new carob cultivars in breeding programmes.