Life Sciences Commons^™

Studies Of Sumoylation In Regulating Mif Stability And Rangap1 Nucleo-Cytoplasmic Shuttling In Controlling Its Sumo Modification, Progga Sen

Wayne State University Dissertations

SUMOylation is an essential post-translational modification that regulates a variety of critical cellular pathways ranging from nuclear transport to protein stability. Accumulating lines of evidence have shown that a perturbation of the SUMOylation pathway is associated with human diseases, especially various types of cancer. Our recent proteomic studies revealed a drastic increase in levels of SUMO2/3 modification on the proinflammatory cytokine MIF in the metastatic breast cancer cell line compared to the non-metastatic control cell line. Interestingly, the increase in levels of both MIF and global SUMO-2/3 modification in the metastatic cells are positively correlated to that of unmodified MIF …

3d Bioprinting Of Stem Cells And Polymer/Bioactive Glass Composite Scaffolds For Bone Tissue Engineering, Caroline Murphy, Krishna Kolan, Wenbin Li, Julie A. Semon, D. E. Day, Ming-Chuan Leu

Biological Sciences Faculty Research & Creative Works

A major limitation of using synthetic scaffolds in tissue engineering applications is insufficient angiogenesis in scaffold interior. Bioactive borate glasses have been shown to promote angiogenesis. There is a need to investigate the biofabrication of polymer composites by incorporating borate glass to increase the angiogenic capacity of the fabri-cated scaffolds. In this study, we investigated the bioprinting of human adipose stem cells (ASCs) with a polycaprolac-tone (PCL)/bioactive borate glass composite. Borate glass at the concentration of 10 to 50 weight %, was added to a mixture of PCL and organic solvent to make an extrudable paste. ASCs suspended in Matrigel …

Impact Of The Capillary Pressure-Saturation Pore-Size Distribution Parameter On Geological Carbon Sequestration Estimates, Chu-Lin Cheng, Edmund Perfect, T. Edward Yu, Michael J. Gragg

School of Earth, Environmental, & Marine Sciences Faculty Publications

Cost estimates for geologic carbon sequestration (GCS) are vital for policy and decision makers evaluating carbon capture and storage strategies. Numerical models are often used in feasibility studies for the different stages of carbon injection and redistribution. Knowledge of the capillary pressure-saturation function for a selected storage rock unit is essential in applications used for simulating multiphase fluid flow and transport. However, the parameters describing these functions (e.g. the van Genuchten m pore size distribution parameter) are often not measured or neglected compared to other physical properties such as porosity and intrinsic permeability. In addition, the use of average …

Linear And Nonlinear Effects Of Temperature And Precipitation On Ecosystem Properties In Tidal Saline Wetlands, Laura C. Feher, Michael J. Osland, Kereen T. Griffith, James B. Grace, Rebecca J. Howard, Camille L. Stagg, Nicholas M. Enwright, Ken W. Krauss, Christopher A. Gabler, Richard H. Day, Kerrylee Rogers

School of Earth, Environmental, & Marine Sciences Faculty Publications

Climate greatly influences the structure and functioning of tidal saline wetland ecosystems. However, there is a need to better quantify the effects of climatic drivers on ecosystem properties, particularly near climate‐sensitive ecological transition zones. Here, we used climate‐ and literature‐derived ecological data from tidal saline wetlands to test hypotheses regarding the influence of climatic drivers (i.e., temperature and precipitation regimes) on the following six ecosystem properties: canopy height, biomass, productivity, decomposition, soil carbon density, and soil carbon accumulation. Our analyses quantify and elucidate linear and nonlinear effects of climatic drivers. We quantified positive linear relationships between temperature and above‐ground productivity …

The Knowledge And Practice Of Workers Toward Occupational Hazards: Case Study From United Arab Emirates, Fares M. Howari, Yousef Nazzal, Mustaffah Awad

All Works

© 2017, Universidade Federal do Rio de Janeiro. All rights reserved. The focus of this investigation is to assess the level of knowledge, attitude and practice (KAP) of workers on occupational health and safety issues in the industrial town of Mussafah, United Arab Emirates. A combination of personnel interviews in conjunction with t-test and logistic regressions were used to assess the KAP of the workers. Around 98% of the survey results indicate that the computed p-value of the responses is greater than the significance level alpha = 0.05, hence the null hypothesis H0 of the test of normality cannot be …

Animal Sentience And The Precautionary Principle, Jonathan Birch

Animal Sentience

In debates about animal sentience, the precautionary principle is often invoked. The idea is that when the evidence of sentience is inconclusive, we should “give the animal the benefit of the doubt” or “err on the side of caution” in formulating animal protection legislation. Yet there remains confusion as to whether it is appropriate to apply the precautionary principle in this context, and, if so, what “applying the precautionary principle” means in practice regarding the burden of proof for animal sentience. Here I construct a version of the precautionary principle tailored to the question of animal sentience together with a …

Shoring Up The Precautionary Bar, Jon Mallatt

Animal Sentience

I offer four ways to reinforce Birch’s precautionary principle so it can be used effectively and practically in deciding which animals to cover by legislation for humane treatment: (1) add one more credible indicator of sentience to the BAR rule; (2) use phylogenetic classification, not the outdated Linnaean classification, to test which animal clades have sentience; (3) disentangle the pain of suffering from sentience; and (4) reconsider the sentient status of decapods and insects to remove potential inconsistencies in the proposed framework.

What Harmful Practices? The Material Scope Of Animal Protection Legislation, Eze Paez

Animal Sentience

Jonathan Birch proposes a criterion for the subjective scope of animal protection legislation. He says nothing about its material scope: which harmful practices it should regulate. I argue, first, that most moral views would agree that the worst forms of animal exploitation should be legally forbidden, even if there will inevitably be disagreement about some cases of animal experimentation. I also argue that, when feasible, there should be legal provisions to help wild animals.

The Value Of Bayesian Statistics For Assessing Credible Evidence Of Animal Sentience, Anil K. Seth, Zoltan Dienes

Animal Sentience

Determining what constitutes practically relevant, statistically significant evidence for animal sentience, under the precautionary principle, could be enhanced through Bayesian statistics. A Bayesian approach allows the incorporation of multiple evidence sources through prior probabilities, the tracking of changing evidence across time, and a principled means of adjusting evidentiary bars via Bayes factors.

Chicken Of The Sea, Jonathan Balcombe

Animal Sentience

Marino summarizes research showing that chickens perform cognitively and emotionally at a higher level than previously assumed. Here, I describe capacities of teleost fishes that parallel those of chickens, including the ability to recognize human faces, perspective-taking, and referential communication. Research on chickens and on fishes reveals an emerging trend in cognitive ethology: abilities once thought limited to a scant few highly intelligent non-humans may actually occur broadly across taxa.

Breastfeeding And Motor Development: A Longitudinal Cohort Study, T Grace, W Oddy, M Bulsara, B Hands

Health Sciences Papers and Journal Articles

Background: While there is a large body of work supporting the importance of early feeding practices on cognitive, immunity, behavioural and mental outcomes, few longitudinal studies have focused on motor development. The relationship between duration of breast feeding and motor development outcomes at 10, 14, and 17 years were examined.

Methods: Data were obtained from the Western Australian Pregnancy (Raine) Study. There were 2868 live births recorded and children were examined for motor proficiency at 10 (M = 10.54, SD = 2.27), 14 (M = 14.02, SD = 2.33) and 17 (M = 16.99, SD = 2.97) years using the …

Biological Maturity And The Anthropometric, Physical And Technical Assessment Of Talent Identified U16 Australian Footballers, A Cripps, C Joyce, C Woods, L Hopper

Health Sciences Papers and Journal Articles

This study compared biological maturation, anthropometric, physical and technical skill measures between talent and non-talent identified junior Australian footballers. Players were recruited from the under 16 Western Australian Football League and classified as talent (state representation; n = 25, 15.7 ± 0.3 y) or non-talent identified (non-state representation; n = 25, 15.6 ± 0.4 y). Players completed a battery of anthropometric, physical and technical skill assessments. Maturity was estimated using years from peak height velocity calculations. Binary logistic regression was used to identify the variables demonstrating the strongest association with the main effect of ‘status’. A receiver operating characteristic curve …

The Most Important "Factor" In Producing Clubhead Speed In Golf, C Joyce

Health Sciences Papers and Journal Articles

Substantial experiential research into x-factor, and to a lesser extent crunch-factor has been undertaken with the aim of increasing clubhead speed. However, a direct comparison of the golf swing kinematics associated with each ‘factor’ has not, and possible differences when using a driver compared to an iron. Fifteen low handicap male golfers who displayed a modern swing had their golf swing kinematic data measured when hitting their own driver and five-iron, using a 10-camera motion analysis system operating at 250 Hz. Clubhead speed was collected using a validated launch monitor. No between-club differences in x-factor and crunch-factor existed. Correlation analyses …

Community-Based Prescribing For Impetigo In Remote Australia: An Opportunity For Antimicrobial Stewardship, S Oliver, J Cush, J Ward

Health Sciences Papers and Journal Articles

Background: To support antibiotic prescribing for both hospital and community-based health professionals working in remote North Western Australia, a multidisciplinary Antimicrobial Stewardship (AMS) Committee was established in 2013. This Committee is usually focused on hospital-based prescribing. A troubling increase in sulfamethoxazole/ trimethoprim resistance in Staphylococcus aureus antibiograms from 9 to 18% over 1 year prompted a shift in gaze to community prescribing.

What we did: Finding a paucity of relevant research, we first investigated contextual factors influencing local prescribing. We also designed a systematic survey of experts with experience relevant to our setting using a structured response survey (12 questions) …

The Improving Rural Cancer Outcomes Trial: A Cluster-Randomised Controlled Trial Of A Complex Intervention To Reduce Time To Diagnosis In Rural Cancer Patients In Western Australia, Jon D. Emery, Victoria Gray, Fiona M. Walter, Shelley Cheetham, Emma J. Croager, Terry Slevin, Christobel Saunders, Timothy Threlfall, Kirsten Auret, Anna K. Nowak, Elizabeth Geelhoed, Max Bulsara, C D'Arcy J. Holman

Health Sciences Papers and Journal Articles

Background: Rural Australians have poorer survival for most common cancers, due partially to later diagnosis. Internationally, several initiatives to improve cancer outcomes have focused on earlier presentation to healthcare and timely diagnosis. We aimed to measure the effect of community- based symptom awareness and general practice-based educational interventions on the time to diagnosis in rural patients presenting with breast, prostate, colorectal or lung cancer in Western Australia.

Methods: 2_2 factorial cluster randomised controlled trial. Community Intervention: cancer symptom awareness campaign tailored for rural Australians. GP intervention: resource card with symptom risk assessment charts and local cancer referral pathways implemented through …

Physical Activity, Body Mass Index, And Clustered Metabolic Risk In U.S. Adolescents: 2007-2012 Nhanes., Bethany Dawn Williams

UNF Graduate Theses and Dissertations

Objectives: To examine variation in clustered metabolic risk (cMetS) in adolescents classified as not overweight/active (NOA), not overweight/not active (NONA), overweight/active (OA), and overweight/not active (ONA).

Background: While studies to date have shown that children and adolescents who meet the current physical activity (PA) recommendations and maintain a healthy body weight demonstrate significantly lower cardiometabolic risk, there are some studies that suggest the relationship between PA and metabolic risk may be mediated by adiposity.

Methods: The sample included adolescent participants (n=875; 12-17 years) of the 2007-2012 National Health and Nutrition Examination Survey (NHANES). The cMetS score included triglycerides, high-density lipoprotein …

Application Of A Coupled Human Natural System Framework To Organize And Frame Challenges And Opportunities For Biodiversity Conservation On Private Lands, John E. Quinn, Jesse Wood

Biology Publications

Conservation science addresses the complementary goals of preventing future biodiversity loss while sustaining critical human foundations. In this paper we use two case studies focused on land management to discuss how private lands conservation can be more effective by considering how planning and decision making reflects a coupled human and natural system (CHANS). The first case study focuses on conservation easements in the temperate forests of eastern United States; the second focuses on conservation opportunities in Midwestern agroecosystems, in particular the value of agroforestry. For each case study we discuss the natural and human subsystems, how elements and interactions within …

Mast Cell Activation By Diverse Stimuli Can Be Suppressed By Steroid Therapy And Targeting The Fyn-Stat5b Cascade, Anuya Paranjape

Theses and Dissertations

Mast cells are critical effectors of allergic disease that can be activated by numerous stimuli. We have examined mast cell control by the inflammatory cytokine, IL-33, as well as IgG. In the first study reported here, we found that the synthetic glucocorticoid, dexamethasone, potently and rapidly suppressed IL-33-induced cytokine production from murine bone marrow–derived and peritoneal mast cells, as well as human mast cells. Dexamethasone also antagonized IL-33-mediated enhancement of IgE-induced cytokine production and migration. Although dexamethasone had no effect on IL-33-induced phosphorylation of MAP kinases or NFκB p65 subunit, it antagonized AP-1 and NFκB-mediated transcriptional activity. Finally, intraperitoneal administration …

Nitrosative Stress Sensing In Porphyromonas Gingivalis: Structure And Function Of The Heme Binding Transcriptional Regulator Hcpr, Benjamin R. Belvin

Theses and Dissertations

Porphyromonas gingivalis, a Gram negative anaerobe implicated in the progression of periodontal disease, is capable of surviving and causing infection despite high levels of reactive nitrogen species found in the oral cavity due to its efficient nitrosative stress response. HcpR is an important sensor-regulator that plays a vital step in the initiation of the nitrosative stress response in many Gram negative anaerobic bacteria. We employ a combination of X-ray crystallography, SAXS, resonance Raman spectroscopy, UV-Vis spectroscopy, and molecular biology techniques to better understand this key regulator. Knockout of the hcpR gene in W83 P. gingivalis results in the inability of …

The Effect Of Chronic Nutrient Addition From Wastewater On Forest Ecosystems At The Rice Rivers Center, Michael Beck

Theses and Dissertations

Wastewater application to land can be a useful tool for mitigating impacts of nutrient enrichment on aquatic systems. A land application treatment system at VCU’s Rice Rivers Center in Charles City County, VA provided an opportunity to study the impact of wastewater addition on the biogeochemistry of forests representative of the Mid-Atlantic Coastal Plain. Nutrient concentrations in throughfall and leachate were measured at Treatment and Control sites to assess differences in nutrient deposition and retention. Wastewater amended plots from the Walter L. Rice education building received 20-fold (N) and 6-fold (P) higher inputs relative to Control plots and plots located …

Isolation And Spectroscopic Characterization Of Zn(Ii), Cu(Ii), And Pd(Ii) Complexes Of 1,3,4-Thiadiazole-Derived Ligand, Dariusz Karcz, Arkadiusz Matwijczuk, Bozena Boron, Bernadette Creaven, Leszek Fiedor, Andrzej Niewiadomy, Mariusz Gagos

Articles

A series of complexes incorporating Zn(II), Cu(II), and Pd(II) ions, and 4-(5-heptyl-1,3,4-thiadiazol-2-yl)benzene-1,3-diol (L1) as model ligand, was synthesized in order to examine the nature of potential interactions between biologically active ligands, 1,3,4-thiadiazoles and metal ions with proven biological relevance. The structures of the compounds isolated were characterized using a number of spectroscopic methods including IR, Uv–vis, AAS, steady state and time-resolved fluorescence (TRF). The results obtained suggest that the L1-Zn(II) and L1-Pd(II) complexes consist of one molecule of L1 and one acetate ion acting as ligands, while the L1-Cu(II) complex adapts a 2:1 (L1: metal) stoichiometry. The coordination of L1 …

Anecdotes Can Be Evidence Too, Heather Browning

Animal Sentience

Birch’s criterion for the precautionary principle imposes a high evidential standard that many cases will fail to meet. Reliable, relevant anecdotal evidence suggestive of animal sentience should also to fall within the scope of the precautionary principle. This would minimize potential suffering (as happened in the case cephalopods) while further evidence is gathered.

Animal Pain And The Social Role Of Science, Leslie Irvine

Animal Sentience

Assuming that all animals are sentient would mean ending their use in most scientific research. This does not necessarily imply an unscientific or anti-scientific stance. Examining the social role of science reveals its considerable investment in preserving the status quo, including the continued use of animal subjects. From this perspective, the use of animal subjects is a custom that science could move beyond, rather than a methodological requirement that it must defend.

Fishes Are Gaining Academic Respect, Jonathan Balcombe

Animal Sentience

I respond to five commentaries on my 2016 book What a Fish Knows. The commentaries express more harmony than dissent about my interpretation of fishes as cognitive, aware individuals deserving better treatment by humankind.

Regime Shifts And Panarchies In Regional Scale Social-Ecological Water Systems, Lance Gunderson, Barbara A. Cosens, Brian C. Chaffin, Craig A. (Tony) Arnold, Alexander K. Fremier, Ahjond S. Garmestani, Robin Kundis Craig, Hannah Gosnell, Hannah E. Birge, Craig R. Allen, Melinda H. Benson, Ryan R. Morrison, Mark C. Stone, Joseph A. Hamm, Kristine Nemec, Edella Schlager, Dagmar Llewellyn

Tallgrass Prairie Publications and Reports

In this article we summarize histories of nonlinear, complex interactions among societal, legal, and ecosystem dynamics in six North American water basins, as they respond to changing climate. These case studies were chosen to explore the conditions for emergence of adaptive governance in heavily regulated and developed social-ecological systems nested within a hierarchical governmental system. We summarize resilience assessments conducted in each system to provide a synthesis and reference by the other articles in this special feature. We also present a general framework used to evaluate the interactions between society and ecosystem regimes and the governance regimes chosen to mediate …

Linking Nutrient Reduction Practices With Biomass Energy, Eric Giddens, Laura Jackson, Kamyar Enshayan

Tallgrass Prairie Publications and Reports

This report outlines options for prairie biomass harvesting, storage, processing, and conversion equipment, and it provides a payback period calculator to analyze a range of scenarios of biomass production and processing costs versus the cost of propane.

An Effective Computational Method Incorporating Multiple Secondary Structure Predictions In Topology Determination For Cryo-Em Images, Abhishek Biswas, Desh Ranjan, Mohammad Zubair, Stephanie Zeil, Kamal Al Nasr, Jing He

Computer Science Faculty Publications

A key idea in de novo modeling of a medium-resolution density image obtained from cryo-electron microscopy is to compute the optimal mapping between the secondary structure traces observed in the density image and those predicted on the protein sequence. When secondary structures are not determined precisely, either from the image or from the amino acid sequence of the protein, the computational problem becomes more complex. We present an efficient method that addresses the secondary structure placement problem in presence of multiple secondary structure predictions and computes the optimal mapping. We tested the method using 12 simulated images from alpha-proteins and …

Freshwater Future: The Influence Of Exposure To Extreme Summer Rainfall Events On The Resistance And Recovery Patterns Of An Estuarine Seagrass, Chanelle Webster

Theses : Honours

Restoring and maintaining the ecological resilience of seagrass ecosystems will be a major challenge of the 21st century. The decline of seagrasses worldwide is attributed to the erosion or elimination of their ecological resilience driven by human impacts, extreme climate events and climate change. Ecological resilience refers to the ability of ecosystems to resist or recover from disturbances whilst maintaining their integral structure and function. Seagrass resilience is influenced by life history, meadow form (transitory or enduring) and habitat type. The purpose of this honours project was to investigate the influence of extreme climate events and meadow form on a …

Opuntioid Cacti Management Guide: Best Practice Information For Austrocylindropuntia, Cylindropuntia And Opuntia Species In Australia, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity published reports

Key points:

• Australia does not have any native cacti.
• Opuntioid cacti impact on Australia environmentally, agriculturally and aesthetically, as well as posing a risk to animal welfare and human safety.
• Invasive cacti are drought resistant and hardy, surviving in low rainfall environments.
• All species spread vegetatively through the rooting of cladodes (stem segments), fruits or flowers. Some species also spread by seed.
• Opuntioid cacti vary significantly in their form and habit, ranging from low-growing shrubs under 50 cm to erect trees up to approximately 6 m tall.
• Australian rangelands are especially vulnerable to cacti invasion.
• The cost of control often …

Morphological Variation In The Monardella Odoratissima – Monardella Villosa Complex, Monica Estiandan

University of the Pacific Theses and Dissertations

In this study, morphological characters were investigated to better characterize the species boundaries between members of the Monardella odoratissima – M. villosa complex (Lamiaeceae). Traditionally, it has been very difficult to identify and separate members of this complex. Monardella linoides, M. purpurea, and M. sheltonii populations are distributed in the overlapping boundaries of these two species (M. odoratissima and M. villosa) and considered within the complex as well. Previous studies and floral manuals have found that the vegetative plant parts from these five Monardella species intergrade with the other taxa in the complex throughout their distribution in California and in …