Life Sciences Commons^™

Could Dietary Peroxidized Lipids Provoke An Intestinal Inflammatory Response?, Mitsushita Doomra

Electronic Theses and Dissertations

Inflammatory Bowel Disease and Crohn's disease represent chronic intestinal inflammatory diseases. It is suspected that bacterial infection is one of the causes of gut inflammation. Studies from others as well as from our laboratory have indicated that peroxidized lipids and their decomposition products are pro-inflammatory. As we consume considerable amounts of dietary oxidized lipids (arising from deep frying of vegetable oils), we hypothesize that dietary peroxidized lipids may also lead to intestinal inflammation. To test this hypothesis, intestine from C57BL/6J mice were collected and used in this study. The intestinal epithelial tissue as well as intestinal lymphoid tissues [Peyer's Patches …

Mathematical Modeling Of Heart Rate Deflection Point In Relation To Respiratory Compensation And Treadmill Running Performance, Kayla Baker

Electronic Theses and Dissertations

Heart rate deflection point (HRDP), identified as the second breakpoint in the "intensity / heart rate" relationship, is indicative of the anaerobic threshold (AT). This point can be determined via bi-segmental linear regression (2SEG) or through use of the maximal distance model (D-max); however, the relationship between these methods has yet to be investigated 2. Purpose: To compare the use of 2SEG and D-max methods to determine HRDP and to examine the relationship between these values with a metabolic threshold, respiratory compensation point (RCP), as well as running performance [5,000 meter treadmill time trial (5Ktime)]. Methods: Nineteen recreationally active men …

Implications Of Galling Herbivory On Ground Thaw In Canada’S Northern Boreal Forest, Allison Mcmanus

Theses and Dissertations (Comprehensive)

Canada’s northern boreal forest has faced significant climate warming over the past century. This change can have direct and indirect effects on forest dynamics, including altering the disturbance patterns of herbivores and changing the abundance of natural enemies. Warmer air temperatures can impact the distribution and survival of arthropods, both novel species and resident natural enemies. Gall-inducing mites are a resident natural enemy in high latitude forests and have been shown to drive dramatic reductions in gas-exchange rates in infested plants. Physiological reductions in plants can directly affect the shrub energy balance and underlying soil processes. This can have major …

The Sublethal Physiological Effects Of Exposure To Copper And Silver Mixtures On Rainbow Trout (Oncorhynchus Mykiss), Wesley Truong

Theses and Dissertations (Comprehensive)

The mechanisms behind metal-metal interactions in freshwater environments are currently not well understood. Freshwater environments consist of many different types of metals, from those naturally present such as copper (Cu) and those that originate from anthropogenic sources like silver (Ag). Both Cu and Ag use apical sodium (Na⁺) channels for uptake into the gills of freshwater fish. In the gills, the mechanisms of Cu²⁺ and Ag⁺ toxicity appear to be similar to one another, which is by inhibiting Na⁺ /potassium (K⁺)- adenosine triphosphatase (NKA) and carbonic anhydrase (CA). Inhibition of NKA and CA …

Changes In Brain Water Content In The Rainbow Trout (Oncorhynchus Mykiss) And In The Goldfish (Carassius Auratus) Due To High External Ammonia Exposure, Phillip Q.H. Pham-Ho

Theses and Dissertations (Comprehensive)

In fishes, hyperammonemia may occur following feeding or exposure to abnormally high concentrations of environmental ammonia due to sewage effluents, agricultural run-off and in crowded aquaculture pens. Increased internal ammonia can result in hyperactivity, convulsions, coma and death. In mammals, it is also associated with potentially fatal brain edema, in which the accumulation of intracellular water results in swelling, increased intracranical pressure and herniation leading to death. Recently it was shown that rainbow trout (Oncorhynchus mykiss) and goldfish (Carassius auratus) experience brain swelling following exposure to high external ammonia (HEA). However, the mechanism of ammonia-induced brain …

The Kinetic Characterization Of Two Putative Galactosidases, Bt2857 And Sco6594, And The Preliminary Analyses Of Sco6595-97, Maria Ngo

Theses and Dissertations (Comprehensive)

Bacteroides thetaiotaomicron is a prolific bacterium found in the distal intestinal tract of humans that possesses the ability to breakdown complex polysaccharides through the release of carbohydrate-active enzymes (CAZymes). Overall, the saccharolytic prowess of B. thetaiotaomicron presents an intriguing model for understanding microbial-host nutrient exchange within the gut and to identify novel mechanisms for accessing carbohydrates. The successful expression, purification, and preliminary kinetic characterization of BT2857 are reported. BT2857 was found to be enzymatically active against the artificial substrate, 4-nitrophenyl β-D-galactopyranoside (pNP-Gal); suggesting its role as a putative β-galactosidase and allowed for the quantification of BT2857 activity by the determination …

Causes And Consequences Of Variation In Female Mate Choice And Its Relation To Sexual Conflict In Drosophila Melanogaster, David Cs Filice

Theses and Dissertations (Comprehensive)

Female mate choice is a significant driving force of evolutionary change and can explain the evolution of exaggerated male traits and/or displays, and dimorphism between the sexes. Females are thought to choose mates based on the greatest provision of direct or indirect benefits. Despite this, we often still see substantial individual variation in female mate choice behaviours both within and across populations. Recent studies suggest that female mate choice is a complex decision-making process that involves many context-dependent factors. However, the precise sources of this variation, such as previous mating experience, are not completely understood. In Drosophila melanogaster, mating …

The 2d:4d Ratio, Handedness, And Sex Across The Age Span, Helen A M Protopapas Ms.

Theses and Dissertations (Comprehensive)

The ratio of the difference between the 2nd and 4th digits of the hand (2D:4D ratio) has been demonstrated to be an indirect indicator of prenatal testosterone levels. Prenatal testosterone has been found to play a role in brain development in utero, and thus may influence lateral asymmetries, such as handedness. Consequently, one of the aims of the current study was to examine relationships between the 2D:4D ratio, hand preference, and hand performance with the factors of sex (males and females), handedness (right handers and left handers), and age considered. A total of 104 participants were tested, 90 right handers …

Validation Of Type 2 Diabetes Risk Variants Identified By Genome-Wide Association Studies In Northern Han Chinese, Ping Rao, Yong Zhou, Siqi Ge, Anxin Wang, Xinwei Yu, Mohamed Ali Alzain, Andrea K. Veronica, Jing Qiu, Manshu Song, Jie Zhang, Hao Wang, Honghong Fang, Qing Gao, Youxin Wang, Wei Wang

Research outputs 2014 to 2021

Background:

More than 60 genetic susceptibility loci associated with type 2 diabetes mellitus (T2DM) have been established in populations of Asian and European ancestry. Given ethnic differences and environmental factors, validation of the effects of genetic risk variants with reported associations identified by Genome-Wide Association Studies (GWASs) is essential. The study aims at evaluating the associations of T2DM with 29 single nucleotide polymorphisms (SNPs) from 19 candidate genes derived from GWASs in a northern Han Chinese population.

Method:

In this case-control study, 461 T2DM-diagnosed patients and 434 controls were recruited at the Jidong oil field hospital (Hebei, China) from January …

Reducing The Risk Of Invasive Forest Pests And Pathogens: Combining Legislation, Targeted Management And Public Awareness, Maartje J. Klapwijk, Anna J. M. Hopkins, Louise Eriksson, Maria Pettersson, Martin Schroeder, Ake Lindlow, Jonas Ronnberg, E Carina H. Keskitalo, Marc Kenis

Research outputs 2014 to 2021

Intensifying global trade will result in increased numbers of plant pest and pathogen species inadvertently being transported along with cargo. This paper examines current mechanisms for prevention and management of potential introductions of forest insect pests and pathogens in the European Union (EU). Current European legislation has not been found sufficient in preventing invasion, establishment and spread of pest and pathogen species within the EU. Costs associated with future invasions are difficult to estimate but past invasions have led to negative economic impacts in the invaded country. The challenge is combining free trade and free movement of products (within the …

Proof Of Acacia Nilotica Stand Expansion In Bekol Savanna, Baluran National Park, East Java, Indonesia Through Remote Sensing And Field Observations, Sutomo, Eddie Van Etten, Luthfi Wahab

Research outputs 2014 to 2021

One of woody species that is known to inhabit certain savanna ecosystems is Acacia nilotica. The Acacia nilotica tree is widespread in the northern savannah regions, and its range extends from Mali to Sudan and Egypt. Acacia nilotica was first introduced to Java Island in 1850. It then spread to Bali, East Nusa Tenggara, Timor and Papua. Found in grasslands, savanna is reported as important colonizer at Baluran National Park in East Java and Wasur National Park Papua. We conducted Vegetation analysis in three areas of the Baluran Savanna namely: Grazed, burned and unburnt. Our observation result analysis showed that …

Key Biogeochemical Factors Affecting Soil Carbon Storage In Posidonia Meadows, Oscar Serrano, Aurora M. Ricart, Paul S. Lavery, Miguel-Angel Mateo, Ariane Arias-Ortiz, Pere Masque, Mohammad Rozaimi, Andy D. L. Steven, Carlos Duarte

Research outputs 2014 to 2021

Biotic and abiotic factors influence the accumulation of organic carbon (C_org) in seagrass ecosystems.We surveyed Posidonia sinuosa meadows growing in different water depths to assess the variability in the sources, stocks and accumulation rates of C_org. We show that over the last 500 years, P. sinuosa meadows closer to the upper limit of distribution (at 2-4m depth) accumulated 3- to 4-fold higher C_org stocks (averaging 6.3 kgC_org m^-2) at 3- to 4-fold higher rates (12.8 gC_org m^-2 yr^-1/ compared to meadows closer to the deep limits of distribution …

Sensitivity Of Composite Scores To Amyloid Burden In Preclinical Alzheimer's Disease: Introducing The Z-Scores Of Attention, Verbal Fluency, And Episodic Memory For Nondemented Older Adults Composite Score, Yen Ying Lim, Peter J. Snyder, Robert H. Pietrzak, Albulene Ukiqi, Victor L. Villemagne, David Ames, Olivier Salvado, Pierrick Bourgeat, Ralph N. Martins, Colin L. Masters, Christopher C. Rowe, Paul Maruff

Research outputs 2014 to 2021

Introduction:

Cognitive composite scores developed for preclinical Alzheimer's disease (AD) often consist of multiple cognitive domains as they may provide greater sensitivity to detect β-amyloid (Aβ)-related cognitive decline than episodic memory (EM) composite scores alone. However, this has never been empirically tested. We compared the rate of cognitive decline associated with high Aβ (Aβ+) and very high Aβ (Aβ++) in cognitively normal (CN) older adults on three multidomain cognitive composite scores and one single-domain (EM) composite score.

Methods:

CN older adults (n = 423) underwent Aβ neuroimaging and completed neuropsychological assessments at baseline, and at 18-, 36-, 54-, …

Asal Usul Formasi Savana: Tinjauan Dari Nusa Tenggara Timur Dan Hasil Penelitian Di Baluran Jawa Timur [Origin Of Savanna Formation: Literature Review From East Nusa Tenggara And Research Results From Baluran East Java Indonesia], Sutomo

Research outputs 2014 to 2021

Savannas are ecosystems mostly confined to tropical and subtropical regions that are characterized by a continuous cover of C₄ grasses that have different characteristics based on seasonality and where woody plants are also an important feature, but with sparse cover and no closed canopy. This is common formation in the driest part of Indonesia such as East Nusa Tenggara, however savana can also be found in Java Island: Baluran Savanna in East Java. There has been considerable amount of debat among scientists, botanists in Indonesia regarding the origin of Indonesian savanna. Using literature study and also field observation at …

Location And Associated Carbon Storage Of Erosional Escarpments Of Seagrass Posidonia Mats, Oscar Serrano, Paul Lavery, Lourdes Lopez-Merino, Enric Ballesteros, Miguel A. Mateo

Research outputs 2014 to 2021

Seagrasses of the genus Posidonia can form an irregular seascape due to erosional processes exposing thick walls of organic matter-rich soils. However, little is known about the location and characteristics of these particular formations. Here we provide comprehensive estimates of organic carbon (C_org) storage in Posidonia oceanica and Posidonia australis meadows, while providing insight into their location and mechanisms of formation, and highlighting future research directions. Erosional reef escarpments are restricted to shallow highly productive P. oceanica meadows from the Mediterranean Sea and P. australis meadows from the Indian Ocean, and sustain the existence of C_org-rich …

Fine Particulate Air Pollution And Hospital Emergency Room Visits For Respiratory Disease In Urban Areas In Beijing, China, In 2013, Qin Xu, Xia Li, Shuo Wang, Chao Wang, Fangfang Huang, Qi Gao, Lijuan Wu, Lixin Tao, Jin Guo, Wei Wang, Xiuhua Guo

Research outputs 2014 to 2021

Background

Heavy fine particulate matter (PM_2.5) air pollution occurs frequently in China. However, epidemiological research on the association between short-term exposure to PM_2.5 pollution and respiratory disease morbidity is still limited. This study aimed to explore the association between PM_2.5 pollution and hospital emergency room visits (ERV) for total and cause-specific respiratory diseases in urban areas in Beijing. Methods Daily counts of respiratory ERV from Jan 1 to Dec 31, 2013, were obtained from ten general hospitals located in urban areas in Beijing. Concurrently, data on PM_2.5 were collected from the Beijing Environmental Protection Bureau, …

Regular Fat And Reduced Fat Dairy Products Show Similar Associations With Markers Of Adolescent Cardiometabolic Health, Theresa A. O'Sullivan, Alexandra P. Bremmer, Trevor A. Mori, Lawrence J. Beilin, Charlotte Wilson, Katherine Hafekost, Gina L. Ambrosini, Rae Chi Huang, Wendy H. Oddy

Research outputs 2014 to 2021

Reduced fat dairy products are generally recommended for adults and children over the age of two years. However, emerging evidence suggests that dairy fat may not have detrimental health effects. We aimed to investigate prospective associations between consumption of regular versus reduced fat dairy products and cardiometabolic risk factors from early to late adolescence. In the West Australian Raine Study, dairy intake was assessed using semi-quantitative food frequency questionnaires in 860 adolescents at 14 and 17-year follow-ups; 582 of these also had blood biochemistry at both points. Using generalized estimating equations, we examined associations with cardiometabolic risk factors. Models incorporated …

Autoantibody Production In Cancer—The Humoral Immune Response Toward Autologous Antigens In Cancer Patients, Pauline Zaenker, Elin Solomonovna Gray, Melanie Ruth Ziman

Research outputs 2014 to 2021

A link between autoimmune responses and cancer via autoantibodies was first described in the 1950s. Since, autoantibodies have been studied for their potential use as cancer biomarkers, however the exact causes of their production remain to be elucidated. This review summarizes current theories of the causes of autoantibody production in cancer, namely:

1) defects in tolerance and inflammation,

2) changes in protein expression levels,

3) altered protein structure, and

4) cellular death mechanisms.

We also highlight the need for further research into this field to improve our understanding of autoantibodies as biomarkers for cancer development and progression.

Coastal Connectivity And Spatial Subsidy From A Microbial Perspective, Christin Säwström, Glenn A. Hyndes, Bradley D. Eyre, Megan J. Huggett, Matthew W. Fraser, Paul S. Lavery, Paul G. Thomson, Flavia Tarquinio, P. D. Steinberg, Bonnie Laverock

Research outputs 2014 to 2021

The transfer of organic material from one coastal environment to another can increase production in recipient habitats in a process known as spatial subsidy. Microorganisms drive the generation, transformation, and uptake of organic material in shallow coastal environments, but their significance in connecting coastal habitats through spatial subsidies has received limited attention. We address this by presenting a conceptual model of coastal connectivity that focuses on the flow of microbially mediated organic material in key coastal habitats. Our model suggests that it is not the difference in generation rates of organic material between coastal habitats but the amount of organic …

Superior Inhibitory Control And Resistance To Mental Fatigue In Professional Road Cyclists, Kristy Martin, Walter Staiano, Paolo Mensaspa, Tom Hennessey, Samuele M. Marcora, Richard J. Keegan, Kevin G. Thompson, David T. Martin, Shona R. Halson, Ben Rattray

Research outputs 2014 to 2021

Purpose:

Given the important role of the brain in regulating endurance performance, this comparative study sought to determine whether professional road cyclists have superior inhibitory control and resistance to mental fatigue compared to recreational road cyclists.

Methods:

After preliminary testing and familiarization, eleven professional and nine recreational road cyclists visited the lab on two occasions to complete a modified incongruent colour-word Stroop task (a cognitive task requiring inhibitory control) for 30 min (mental exertion condition), or an easy cognitive task for 10 min (control condition) in a randomized, counterbalanced cross-over order. After each cognitive task, participants completed a 20-min time …

Accelerating Tropicalization And The Transformation Of Temperate Seagrass Meadows, Glenn A. Hyndes, Kenneth L. Heck, Jnr, Adriana Verges, Euan S. Harvey, Gary A. Kendrick, Paul Lavery, Kathryn Mcmahon, Robert J. Orth, Alan Pearce, Mathew Vanderklift, Thomas Wernberg, Scott Whiting, Shaun Wilson

Research outputs 2014 to 2021

Climate-driven changes are altering production and functioning of biotic assemblages in terrestrial and aquatic environments. In temperate coastal waters, rising sea temperatures, warm water anomalies and poleward shifts in the distribution of tropical herbivores have had a detrimental effect on algal forests. We develop generalized scenarios of this form of tropicalization and its potential effects on the structure and functioning of globally significant and threatened seagrass ecosystems, through poleward shifts in tropical seagrasses and herbivores. Initially, we expect tropical herbivorous fishes to establish in temperate seagrass meadows, followed later by megafauna. Tropical seagrasses are likely to establish later, delayed by …

The Uyghur Population And Genetic Susceptibility To Type 2 Diabetes: Potential Role For Variants In Capn10, Apm1 And Fut6 Genes, Feifei Zhao, Dolikun Mamatyusupu, Youxin Wang, Honghong Fang, Hao Wang, Qing Gao, Hao Dong, Siqi Ge, Xinwei Yu, Jie Zhang, Lijuan Wu, Manshu Song, Wei Wang

Research outputs 2014 to 2021

Genome-wide association studies have successfully identified over 70 loci associated with the risk of type 2 diabetes mellitus (T2DM) in multiple populations of European ancestry. However, the risk attributable to an individual variant is modest and does not yet provide convincing evidence for clinical utility. Association between these established genetic variants and T2DM in general populations is hitherto understudied in the isolated populations, such as the Uyghurs, resident in Hetian, far southern Xinjiang Uyghur Autonomous Region, China. In this case–control study, we genotyped 13 single-nucleotide polymorphisms (SNPs) at 10 genes associated with diabetes in 130 cases with T2DM and 135 …

Linking Vital Rates Of Landbirds On A Tropical Island To Rainfall And Vegetation Greenness, James F. Saracco, Paul Radley, Peter Pyle, Erin Rowan, Ron Taylor, Lauren Helton

Research outputs 2014 to 2021

Remote tropical oceanic islands are of high conservation priority, and they are exemplified by range-restricted species with small global populations. Spatial and temporal patterns in rainfall and plant productivity may be important in driving dynamics of these species. Yet, little is known about environmental influences on population dynamics for most islands and species. Here we leveraged avian capture-recapture, rainfall, and remote-sensed habitat data (enhanced vegetation index [EVI]) to assess relationships between rainfall, vegetation greenness, and demographic rates (productivity, adult apparent survival) of three native bird species on Saipan, Northern Mariana Islands: rufous fantail (Rhipidura rufifrons), bridled white-eye (Zosterops conspicillatus), and …

Inactivation Of Cytomegalovirus In Breast Milk Using Ultraviolet-C Irradiation: Opportunities For A New Treatment Option In Breast Milk Banking, Megan L. Lloyd, Nurul Hod, Jothsna Jayaraman, Elizabeth A. Marchant, Lukas Christen, Peter Chiang, Peter E. Hartmann, Geoffrey R. Shellam, Karen N. Simmer

Research outputs 2014 to 2021

Pasteurized donor human milk is provided by milk banks to very preterm babies where their maternal supply is insufficient or unavailable. Donor milk is currently processed by Holder pasteurization, producing a microbiologically safe product but significantly reducing immunoprotective components. Ultraviolet-C (UV-C) irradiation at 254 nm is being investigated as an alternative treatment method and has been shown to preserve components such as lactoferrin, lysozyme and secretory IgA considerably better than Holder pasteurization. We describe the inactivation of cytomegalovirus, a virus commonly excreted into breast milk, using UV-C irradiation. Full replication was ablated by various treatment doses. However, evidence of viral …

Impact Of Mooring Activities On Carbon Stocks In Seagrass Meadows, Oscar Serrano, R. Ruhon, Paul S. Lavery, Gary A. Kendrick, S. Hickey, Pere Masque, Ariane Arias-Ortiz, Andy D.L. Steven, Carlos M. Duarte

Research outputs 2014 to 2021

Boating activities are one of the causes that threaten seagrass meadows and the ecosystem services they provide. Mechanical destruction of seagrass habitats may also trigger the erosion of sedimentary organic carbon (C_org) stocks, which may contribute to increasing atmospheric CO₂. This study presents the first estimates of loss of C_org stocks in seagrass meadows due to mooring activities in Rottnest Island, Western Australia. Sediment cores were sampled from seagrass meadows and from bare but previously vegetated sediments underneath moorings. The C_org stores have been compromised by the mooring deployment from 1930s onwards, which involved …

Association Between Igf2bp2 Polymorphisms And Type 2 Diabetes Mellitus: A Case-Control Study And Meta-Analysis, Ping Rao, Hao Wang, Honghong Fang, Qing Gao, Jie Zhang, Manshu Song, Yong Zhou, Youxin Wang, Wei Wang

Research outputs 2014 to 2021

Background:

Genome-wide association studies (GWAS) found that IGF2BP2 rs4402960 and rs1470579 polymorphisms were associated with type 2 diabetes mellitus (T2DM) risk. Many studies have replicated this association, but yielded inconsistent results.

Materials and Methods:

A case-control study consisting of 461 T2DM patients and 434 health controls was conducted to detect the genetic susceptibility of IGF2BP2 in a northern Han Chinese population. A meta-analysis was to evaluate the association more precisely in Asians.

Results:

In the case-control study, the carriers of TT genotype at rs4402960 had a higher T2DM risk than the G carriers (TG + GG) (adjusted …

Can Mud (Silt And Clay) Concentration Be Used To Predict Soil Organic Carbon Content Within Seagrass Ecosystems?, Oscar Serrano, Paul Lavery, Carlos Duarte, Gary A. Kendrick, Antoni M. Calfat, P. H. York, Andy D. Steven, Peter I. Macreadie

Research outputs 2014 to 2021

The emerging field of blue carbon science is seeking cost-effective ways to estimate the organic carbon content of soils that are bound by coastal vegetated ecosystems. Organic carbon (C_org) content in terrestrial soils and marine sediments has been correlated with mud content (i.e., silt and clay, particle sizes < 63 µm), however, empirical tests of this theory are lacking for coastal vegetated ecosystems. Here, we compiled data (n =  1345) on the relationship between C_org and mud contents in seagrass ecosystems (79 cores) and adjacent bare sediments (21 cores) to address whether mud can be used to predict soil C_org content. We also combined these data with the δ¹³C signatures of the soil …

Permanent Slides For Morphological Studies Of Small Crustaceans: Serban's Method And Its Variation Applied On Bathynellacea (Malacostraca), Giulia Perina, Ana I. Camacho

Research outputs 2014 to 2021

Morphological studies of small invertebrates often involve the preparation of slides to observe minute body parts under a compound microscope. Preparation should facilitate observation, through traditional optical microscopy, of small surface structures on different planes, like pores, spines and setae. Various methods and techniques, using different mounting media that specialists have adopted to observe and preserve small crustaceans, have their advantages and disadvantages.Within the order Bathynellacea, specimens in the family Bathynellidae are particularly challenging due to their small size (0.5 to 2.25 mm body length) and very delicate exoskeleton, which tends to be completely digested when using common clearing mounting …

Metabolomics Enables Precision Medicine: “A White Paper, Community Perspective”, Richard D. Beger, Warwick B. Dunn, Michael A. Schmidt, Steven S. Cross, Jennifer A. Kirwan, Marta S. Cascante, Lorraine Brennan, David S. Wishart, Matej Orešič, Thomas Hankemeier, David Broadhurst, Andrew N. Lane, Karsten Suhre, Gabi Kastenmüller, Susan J. Sumner, Ines Thiele, Oliver E. Fiehn, Rima Kaddurah- Daouk

Research outputs 2014 to 2021

Introduction:Background to metabolomics:

Metabolomics is the comprehensive study of the metabolome, the repertoire of biochemicals (or small molecules) present in cells, tissues, and body fluids. The study of metabolism at the global or “-omics” level is a rapidly growing field that has the potential to have a profound impact upon medical practice. At the center of metabolomics, is the concept that a person’s metabolic state provides a close representation of that individual’s overall health status. This metabolic state reflects what has been encoded by the genome, and modified by diet, environmental factors, and the gut microbiome. The metabolic profile provides …

In Vitro Transcription Lab, Sarah Oh, Samantha Christ

A with Honors Projects

This is a lab and worksheet created by students of Biology 141 as a project for the A with Honors Program. to demonstrate transcription from DNA to RNA. After completing this exercise, the student should be able to distinguish between yields after electrophoresis, describe which varied substance contributed to the largest yield, and describe the importance of not contaminating the sample.