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Articles 1 - 30 of 133
Full-Text Articles in Life Sciences
Downregulation Of Cystathionine γ Lyase And Endothelial Nitric Oxide Synthase And Reduced Responsiveness Of α1a Adrenergic Receptors In The Kidneys Of Left Ventricular Hypertrophied Wistar Kyoto Rats, Ashfaq Ahmad, Munavvar Abdul Sattar, Hassaan Anwer Rathore, Safia Akhtar Khan, Nor Azizan Abdullah, Edward James Johns
Downregulation Of Cystathionine γ Lyase And Endothelial Nitric Oxide Synthase And Reduced Responsiveness Of α1a Adrenergic Receptors In The Kidneys Of Left Ventricular Hypertrophied Wistar Kyoto Rats, Ashfaq Ahmad, Munavvar Abdul Sattar, Hassaan Anwer Rathore, Safia Akhtar Khan, Nor Azizan Abdullah, Edward James Johns
Turkish Journal of Biology
This article explores the relationship between the renal expression of cystathionine gamma lyase (CSE) and endothelial nitric oxide synthase (eNOS) and the responsiveness of α1A adrenergic receptors in the renal vasculature following left ventricular hypertrophy (LVH) in rats. LVH was established by administering isoprenaline (5 mg/kg, 5 injections subcutaneously, 72 h apart) with 62 mg/L caffeine in drinking water for 2 weeks. Renal vasoconstrictor responses were measured using local administration of adrenergic agonists noradrenaline (NA), phenylephrine (PE), and methoxamine (ME) and the selective α1A adrenergic antagonist 5-methylurapidil (5-MeU). Mean arterial blood pressure was higher (144 ± 9 vs. 116 ± …
Cloning And Abiotic Stress Resistance Analyses Of A New Proline-Glycine-Alaninehistidine-Rich Protein Gene From Ipomoea Batatas (L.) Lam., Xiang Tao, Bin Yong, Huan-Huan Shao, Xin-Rong Ma
Cloning And Abiotic Stress Resistance Analyses Of A New Proline-Glycine-Alaninehistidine-Rich Protein Gene From Ipomoea Batatas (L.) Lam., Xiang Tao, Bin Yong, Huan-Huan Shao, Xin-Rong Ma
Turkish Journal of Biology
Sweet potato [Ipomoea batatas (L.) Lam.] is one of the top six most important crops in the world in terms of production. It has the capacity to tolerate marginal growing conditions better than most other crops. In this study, a proline- and glycine-rich protein encoding gene, IbPGAHRP1, was isolated and characterized from the transcriptome of sweet potato cultivar Xushu 18. Sequence analyses showed that proline, glycine, alanine, and histidine account for 62.6% of the total amino acid residues. Two proline-rich tandem repeats, an alanine-rich repeat, a histidine-rich tandem repeat, and five XYPP motifs were present in IbPGAHRP1. Sequence similarity analysis …
The Aggregatibacter Actinomycetemcomitans Heat Shock Protein Groel Interacts Directly With Human Peripheral Blood T Cells, Ayten Nalbant, Tahsi̇n Saygili
The Aggregatibacter Actinomycetemcomitans Heat Shock Protein Groel Interacts Directly With Human Peripheral Blood T Cells, Ayten Nalbant, Tahsi̇n Saygili
Turkish Journal of Biology
Heat shock family protein GroEL of Aggregatibacter actinomycetemcomitans (Aa) has antigenic properties. We previously demonstrated that A. actinomycetemcomitans GroEL-like protein affects human CD4 T cells by converting them into IL-10 and IFNg double cytokine producing Tbet+ Th1 cells. The objective of this study was to investigate whether or not AaGroEL communicates with T cells directly. To do this, sorted cells from peripheral blood mononuclear cells were stimulated with AaGroEL for 48 h. Flow cytometry was used to measure soluble and intracellular cytokine expression in the cell cultures and detect TLR2 expression on the surface of T cells. Expression of six …
Effect Of Polyamines On Somatic Embryogenesis Via Mature Embryo In Wheat, Murat Aydin, Arash Hossein Pour, Kami̇l Hali̇loğlu, Meti̇n Tosun
Effect Of Polyamines On Somatic Embryogenesis Via Mature Embryo In Wheat, Murat Aydin, Arash Hossein Pour, Kami̇l Hali̇loğlu, Meti̇n Tosun
Turkish Journal of Biology
Plant tissue culture via somatic embryogenesis plays a key role in wheat genetic transformation studies. Therefore, the production of embryogenic calli with a high regeneration capacity is a prerequisite for efficient plant regeneration. Although immature embryos are the best type of explants for plant regeneration via somatic embryogenesis, the use of mature embryos has remarkable advantages compared to immature embryos as explants. However, the regeneration capacity of mature embryos is still lower than that of immature embryos. Plant regeneration from somatic embryos can only occur when they are mature enough. Moreover, the maturation of somatic embryos and their conversion into …
High Levels Of Polyphosphate Kinase Affect Not Only Endotoxin Production But Also Acid Tolerance Of Bacillus Thuringiensis, Tuğrul Doruk, Zeynep Gi̇rgi̇n Ersoy, Mehmet Sali̇m Öncel, Sedef Tunca Gedi̇k
High Levels Of Polyphosphate Kinase Affect Not Only Endotoxin Production But Also Acid Tolerance Of Bacillus Thuringiensis, Tuğrul Doruk, Zeynep Gi̇rgi̇n Ersoy, Mehmet Sali̇m Öncel, Sedef Tunca Gedi̇k
Turkish Journal of Biology
Inorganic phosphate is stored as a polyphosphate (polyP) polymer inside every living cell. This polymer is synthesized by the polyP kinase (PPK) enzyme using the terminal phosphate of ATP as substrate and it performs important functions in the cell. In this study, effects of high levels of PPK on Bacillus thuringiensis subsp. israelensis were analyzed. Recombinant Bti ppk, a PPK overproducer, was found to uptake more phosphate into the cell and produce a consistently higher amount of endotoxin than the wild type under culture conditions including a range of temperatures (25 °C, 30 °C, and 37 °C), pH values (pH …
Copper Enriches Efficacy Of Dp44mt In Breast Cancer Cells, Ufuk Özer
Copper Enriches Efficacy Of Dp44mt In Breast Cancer Cells, Ufuk Özer
Turkish Journal of Biology
Neoplastic cells need essential metals, such as iron and copper, for cellular functions and rapid growth. Metal chelation and changes in their redox cycle in favor of oxidative stress may be critical for making these cells vulnerable to cell death. The HER2-overexpressing breast cancer cell line, MCF7-HER2, and its vehicle control, MCF7-vec cells, were treated with CuCl2, FeSO4, and Dp44mT. Reactive oxygen species (ROS) production in response to these chemicals was detected with flow cytometry, and cell viability was measured by MTT assay in the cells. ROS levels were relatively increased by Dp44mT in the cells, and this was reversed …
Identification And Cloning Of Highly Epitopic Regions Of Clostridium Novyi Alpha Toxin, Najme Gord Noshahri, Mohsen Fathi Najafi, Ali Makhdoumi Kakhki, Behjat Majidi, Mohsen Mehrvarz
Identification And Cloning Of Highly Epitopic Regions Of Clostridium Novyi Alpha Toxin, Najme Gord Noshahri, Mohsen Fathi Najafi, Ali Makhdoumi Kakhki, Behjat Majidi, Mohsen Mehrvarz
Turkish Journal of Biology
The aim of the present study is to provide a strategy for predicting the production of antigenic regions of Clostridium novyi alpha toxin. The selection is based on B-cell epitopes and MHCII binding protein by immunoinformatics tools. The study resulted in identifying antigenic regions in the beginning and middle (3-17 and 965-997 amino acid residues) as suitable binders to MHCII and the carboxyl terminal of the protein (1800-1958 amino acid residues) in B-cell epitopes. The appropriate region in B-cell epitopes was chosen for cloning. The presence of recombinant protein was detected with immunological methods. Subsequently, BALB/C mice were immunized with …
Plantlet Regeneration From Young Leaf Segments Of Curaua (Ananas Erectifolius), An Amazon Species, Carolina Moreira, Helena Andrade, Suzan Bertolucci, Osmar Lameira, Aliyu Mohammed, José Eduardo Pinto
Plantlet Regeneration From Young Leaf Segments Of Curaua (Ananas Erectifolius), An Amazon Species, Carolina Moreira, Helena Andrade, Suzan Bertolucci, Osmar Lameira, Aliyu Mohammed, José Eduardo Pinto
Turkish Journal of Biology
The present study reports the first efficient in vitro regeneration of Ananas erectifolius via indirect organogenesis. Leaf segments (leaf base, middle, and apex) excised from 3- or 5-week-old in vitro plantlets were cultured on 1/4 strength MS medium supplemented with different concentrations and combinations of plant growth regulators. The explants were also exposed to pulse treatment with thidiazuron (TDZ) and 2,4-dichlorophenoxyacetic acid (2,4-D) for different periods of time. The results showed that using juvenile rather than old explants enhanced the frequency of callus induction (35.0% and 16.0%, respectively). Among the explant types tested, only leaf base segments induced calli; the …
High Dietary Fructose Load Aggravates Lipid Metabolism In The Liver Of Wistar Rats Through Imbalance Between Lipogenesis And Fatty Acid Oxidation, Ana Teofilovic, Biljana Bursac, Ana Djordjevic, Danijela Vojnovic Milutinovic, Gordana Matic, Natasa Velickovic
High Dietary Fructose Load Aggravates Lipid Metabolism In The Liver Of Wistar Rats Through Imbalance Between Lipogenesis And Fatty Acid Oxidation, Ana Teofilovic, Biljana Bursac, Ana Djordjevic, Danijela Vojnovic Milutinovic, Gordana Matic, Natasa Velickovic
Turkish Journal of Biology
Fructose ingestion is often associated with hepatic steatosis and hypertriglyceridemia. The homeostasis of hepatic lipids is mainly determined by the interplay of lipogenesis and fatty acid β-oxidation. In this study, we hypothesized that high fructose intake disturbs hepatic lipid metabolism through an imbalance between these processes. Therefore, we analyzed the effects of a 9-week-long consumption of a 60% fructose solution on physiological parameters, glycemia, and blood lipid profiles in male Wistar rats. The expression of key regulators of fatty acid oxidation (FAO) and lipogenesis in the liver were assessed by western blot and quantitative polymerase chain reaction. The results showed …
Preparation And Characterization Of Acellular Porcinepericardium For Cardiovascular Surgery, Ha Le Bao Tran, Trang Thi Huyen Dinh, My Thi Ngoc Nguyen, Quan Minh To, Anh Tho Tuan Pham
Preparation And Characterization Of Acellular Porcinepericardium For Cardiovascular Surgery, Ha Le Bao Tran, Trang Thi Huyen Dinh, My Thi Ngoc Nguyen, Quan Minh To, Anh Tho Tuan Pham
Turkish Journal of Biology
The aim of this study was to fabricate and characterize acellular porcine pericardium that can be used to patch cardiovascular repair. Porcine pericardial tissues were treated with 10 mM Tris-HCl and sodium dodecyl sulfate (SDS) at different concentrations and time intervals. The optimal decellularization protocol was determined according to elimination of cellular components and DNA via histology and DNA quantification, respectively. There were no significant changes in stress and fracture strain after decellularization. Liquid extracts from the decellularized pericardium caused no cytotoxicity towards human fibroblasts, were capable of supporting an appropriate attachment of human endothelial progenitor cells, and did not …
Cytotoxic Effects Of Peanut Phenolic Compounds Possessing Histone Deacetylase Inhibitory Activity On Human Colon Cancer Cell Lines, Somprasong Saenglee, Sanun Jogloy, A. Patanothai, Thanaset Senawong
Cytotoxic Effects Of Peanut Phenolic Compounds Possessing Histone Deacetylase Inhibitory Activity On Human Colon Cancer Cell Lines, Somprasong Saenglee, Sanun Jogloy, A. Patanothai, Thanaset Senawong
Turkish Journal of Biology
Phenolic compounds present in our diet play an important role in colon cancer chemoprevention. Previous results demonstrated that peanut testa extract inhibited both histone deacetylase (HDAC) activity and the growth of colon cancer cells. In this study, four identified phenolic compounds in peanut testae (resveratrol, p-coumaric acid, ferulic acid, and sinapinic acid) were investigated for their HDAC inhibitory and anticancer activities against colon cancer cell lines. In vitro study revealed that resveratrol exhibited the greatest HDAC inhibitory activity. Molecular docking studies demonstrated that all four compounds could bind both HDAC1 and HDAC2. Resveratrol exhibited the most effective antiproliferative activity against …
The Sensitivity Of 5-Formylcytosine To Doxorubicin Regardless Of Dna Damage, Selcen Çeli̇k Uzuner, Chris O'Neill
The Sensitivity Of 5-Formylcytosine To Doxorubicin Regardless Of Dna Damage, Selcen Çeli̇k Uzuner, Chris O'Neill
Turkish Journal of Biology
We have previously shown that DNA damage induced by doxorubicin was associated with an increase in the level of DNA methylation, 5meC, in mouse embryonic fibroblasts using a developed immunostaining methodology. Doxorubicin (also known as adriamycin), which induces double-strand DNA breaks, has been widely used in cancer therapy. Cytosine modifications are dynamically regulated in the genome during lifespan, but abnormal changes in the 5meC pattern can be associated with diseases in particular cancer progressions and DNA damage. One of the side effects of cancer therapy is that chemotherapeutic agents can damage healthy cells as well as cancerous cells. In this …
Selective Suppression Of Tumor Cells By A Tumor-Specific Bicistronic Lentiviral Vector, Saeed Ranjbar, Mohammad Sadegh Hashemzadeh, Shahryar Khoshtinat Nikkhoi, Alireza Farasat, Mahdi Tat, Majdedin Ghalavand, Ruhollah Dorostkar
Selective Suppression Of Tumor Cells By A Tumor-Specific Bicistronic Lentiviral Vector, Saeed Ranjbar, Mohammad Sadegh Hashemzadeh, Shahryar Khoshtinat Nikkhoi, Alireza Farasat, Mahdi Tat, Majdedin Ghalavand, Ruhollah Dorostkar
Turkish Journal of Biology
Lentiviral gene delivery is now considered as a major candidate in the future of cancer therapy. To avoid common side-effects associated with methods of cancer therapy, the survivin promoter shows great promise due to its high expression level in multiple cancers. In this research, using a 2A peptide, Noxa was coexpressed with hemagglutinin neuraminidase (HN) driven by the survivin promoter (Surp). Coding genes of Noxa and HN were connected to the upstream and downstream of a 2A peptide using SOE PCR. After cloning of Noxa-2A-HN and Surp into the lentiviral vector, the final construct was transfected to SK-BR3 tumor cells …
Changes In The Levels Of Liver Hsp70, Plasma Nitric Oxide, And The Antioxidative System In An Experimentally Induced Endotoxemia Mouse Model And The Role Of Reduced Glutathione, Temel İlgün, Kezban Yildiz Dalginli, Canan Gülmez, Onur Ataki̇şi̇
Changes In The Levels Of Liver Hsp70, Plasma Nitric Oxide, And The Antioxidative System In An Experimentally Induced Endotoxemia Mouse Model And The Role Of Reduced Glutathione, Temel İlgün, Kezban Yildiz Dalginli, Canan Gülmez, Onur Ataki̇şi̇
Turkish Journal of Biology
Endotoxin molecules in lipopolysaccharides are among most important molecules that initiate a cascade of events in sepsis/endotoxemia. Lipopolysaccharide exposure may result in strong immune responses, disrupt the intracellular oxidant/antioxidant balance, and cause excessive reactive oxygen species generation. The purpose of the study was to examine if reduced glutathione (GSH) has a protective role against lipopolysaccharides. The effects of lipopolysaccharide (LPS) or GSH alone or in combination on the levels of the plasma antioxidant system, NO, and liver HSP70 were investigated. A total of 100 Swiss albino mice were divided into 4 groups as Group I (control), Group II (20 μg/kg …
Cytotoxicity And Antibiofilm Activity Of Sio2/Cellulose Derivative Hybrid Materials Containing Silver Nanoparticles, Tsvetelina Angelova, Nadezhda Rangelova, Veselina Uzunova, Nelly Georgieva, Tonya Andreeva, Albena Momchilova, Rumiana Tzoneva, Rudolf Müller
Cytotoxicity And Antibiofilm Activity Of Sio2/Cellulose Derivative Hybrid Materials Containing Silver Nanoparticles, Tsvetelina Angelova, Nadezhda Rangelova, Veselina Uzunova, Nelly Georgieva, Tonya Andreeva, Albena Momchilova, Rumiana Tzoneva, Rudolf Müller
Turkish Journal of Biology
Hybrid materials based on tetraethyl orthosilicate (TEOS), hydroxypropyl cellulose (HPC), or hydroxypropyl methyl cellulose (HPMC) with silver nanoparticles were synthesized. They were analyzed and characterized using differential thermal analysis and thermogravimetry, atomic force microscopy, and static contact angle measurements. It was experimentally demonstrated that the silver-doped hybrid materials have pronounced antibacterial behavior by studying the reduction of Pseudomonas aeruginosa PAO1 biofilm formation on the tested materials. The results revealed biofilm reduction of 35.7% and 30% by SiO2/HPC/2.5% Ag and SiO2/HPMC/2.5% Ag hybrid materials, respectively, compared to the control. Cytotoxicity of examined materials and actin cytoskeleton organization of fibroblasts seeded on …
Comparison Of Phototoxic Effects Of Hypericin-Mediated Photodynamictherapy In Ht-29 And Caco-2 Colon Cancer Cells, Aysun Kiliç Süloğlu, Güldeni̇z Selmanoğlu, Şükran Yilmaz, Hande Canpinar
Comparison Of Phototoxic Effects Of Hypericin-Mediated Photodynamictherapy In Ht-29 And Caco-2 Colon Cancer Cells, Aysun Kiliç Süloğlu, Güldeni̇z Selmanoğlu, Şükran Yilmaz, Hande Canpinar
Turkish Journal of Biology
Hypericin (HYP) is a plant-derived photosensitizer. HYP is preferentially taken up by tumor cells. We designed this study to compare HYP-mediated photodynamic therapy (PDT) in HT-29 and Caco-2 colon cell lines. Cells were treated with 0.04, 0.08, and 0.15 μM HYP concentrations and irradiated. The effect of HYP on metabolic profiles, alterations in lactate dehydrogenase (LDH) leakage, and cell cycle progression was investigated for the first time. Changes in glucose consumption, lactate production, and LDH leakage were analyzed. HYP-induced cell death was quantified by double staining (acridine orange/propidium iodide) and alterations in cell cycle regulation were analyzed with flow cytometry …
Higher Expression Level Of Bat3 Is Associated With Silencing Of Themidn Gene In Primary Mouse Cardiomyocytes, Bi̇lge Şadan Selçuk, Ayşe Evri̇m Bayrak, Ni̇han Ünaltuna
Higher Expression Level Of Bat3 Is Associated With Silencing Of Themidn Gene In Primary Mouse Cardiomyocytes, Bi̇lge Şadan Selçuk, Ayşe Evri̇m Bayrak, Ni̇han Ünaltuna
Turkish Journal of Biology
Midnolin (Midn) is a developmentally regulated nucleolar protein that was originally identified in the mesencephalon of developing mouse embryos. Additionally, Midn expression was detected in various adult tissues and was associated with glucokinase activity in pancreatic beta cells and with the regulation of folliculogenesis in ovaries. In this study, our aim was to analyze the expression and function of the Midn gene in cardiomyocyte cells. We constructed a primary neonatal mouse cardiomyocyte cell culture and analyzed the expression of possible Midn-associated genes after short interfering RNA (siRNA)-mediated gene silencing for Midn. After 24 h of silencing, we observed that the …
Enhancement Of Direct Shoot Regeneration And Determination Of Bioactivesecondary Metabolites In Leaves Of Galega Officinalis L., Fatma Pehli̇van Karakaş, Günce Ci̇ngöz, Arzu Türker
Enhancement Of Direct Shoot Regeneration And Determination Of Bioactivesecondary Metabolites In Leaves Of Galega Officinalis L., Fatma Pehli̇van Karakaş, Günce Ci̇ngöz, Arzu Türker
Turkish Journal of Biology
Galega officinalis L. is a medicinal plant being used as a galactagogue and an antidiabetic in folk medicine. A new effective micropropagation protocol was established from leaf, stem, node, and root explants of G. officinalis for in vitro plant regeneration and callus induction systems. Murashige and Skoog (MS) medium supplemented with 55 various concentrations and 4 different combinations [benzyl adenine (BA)/naphthalene acetic acid (NAA), BA/indole-3-acetic acid (IAA), thidiazuron (TDZ)/indole-3-butyric acid (IBA), and TDZ/IAA] of plant growth regulators was used for the regeneration systems. The regenerated shoots were observed on only nodal explants through direct organogenesis at the 35th day. The …
The Effects Of Nutrient And Macronutrient Stress On Certain Secondary Metabolite Accumulations And Redox Regulation In Callus Cultures Of Bellis Perennis L., Günce Ci̇ngöz, Fatma Pehli̇van Karakaş
The Effects Of Nutrient And Macronutrient Stress On Certain Secondary Metabolite Accumulations And Redox Regulation In Callus Cultures Of Bellis Perennis L., Günce Ci̇ngöz, Fatma Pehli̇van Karakaş
Turkish Journal of Biology
The main purpose of this study was to investigate the effects of nutrient and macronutrient stress on complex variations in the phenolic profile and antioxidant status of Bellis perennis L. callus cultures. To obtain stress conditions, callus cultures were grown under insufficient nutrient conditions (different strengths of MS medium) or under conditions of limiting calcium (-Ca), magnesium (-Mg), or both (-Ca & -Mg). Phenolic content of callus cultures obtained from different stress treatments was detected quantitatively by LC-ESI-MS/MS analysis. The major compound was chlorogenic acid in all treatments. The accumulation of hesperidin, rutin hydrate, and chlorogenic acid was significantly induced …
Carbohydrate Deprivation Upsurges The Expression Of Genes Responsible For Programmed Cell Death In Inflorescence Tissues Of Oil Palm (Elaeis Guineensis Jacq.), Walter Ajambang, Hugo Volkaert, Sudarsono Sudarsono
Carbohydrate Deprivation Upsurges The Expression Of Genes Responsible For Programmed Cell Death In Inflorescence Tissues Of Oil Palm (Elaeis Guineensis Jacq.), Walter Ajambang, Hugo Volkaert, Sudarsono Sudarsono
Turkish Journal of Biology
The aim of this present study was to analyze global gene expression from a developing inflorescence of oil palm under complete defoliation stress. We used the Illumina platform to sequence six cDNA libraries of defoliated and nondefoliated tissues. The frequency of differentially expressed genes (DEGs) decreased with increasing fold change at P ≤ 0.05. Gene ontology analysis revealed that about 15% of all DEGs were responsible for stress response while 13.9% of genes were responsible for cell organization and developmental processes. DAVID functional analysis clustering of DEGs identified a higher enrichment score (ES) for cellular response to stress (ES = …
Trpc1 Ion Channel Gene Regulates Store-Operated Calcium Entryand Proliferation In Human Aortic Smooth Muscle Cells, Yasemi̇n Erac, Cigdem Selli, Meti̇ner Tosun
Trpc1 Ion Channel Gene Regulates Store-Operated Calcium Entryand Proliferation In Human Aortic Smooth Muscle Cells, Yasemi̇n Erac, Cigdem Selli, Meti̇ner Tosun
Turkish Journal of Biology
This study investigates whether the reciprocal changes in transient receptor potential canonical (TRPC)1 and TRPC6 expressions, which have previously been observed in aging rat aorta, are functional in store-operated calcium (SOC) entry and proliferation in human vascular smooth muscle cells. TRPC1 levels were modulated via silencing and overexpression vectors in human primary aortic smooth muscle cells. Following TRPC1 gene modulation, TRPC1 and TRPC6 expression levels were measured using quantitative real-time RT-PCR. In functional analyses, real-time changes in intracellular calcium levels and cell proliferation were determined. Microarray analysis was performed to identify genes associated with functional alterations following TRPC1 silencing. TRPC1 …
Pepper (Capsicum Annuum L.) Anther Culture: Fundamentalresearch And Practical Applications, Teodora Popova Irikova, Spyridon Kintzios, Stanislava Grozeva, Velichka Rodeva
Pepper (Capsicum Annuum L.) Anther Culture: Fundamentalresearch And Practical Applications, Teodora Popova Irikova, Spyridon Kintzios, Stanislava Grozeva, Velichka Rodeva
Turkish Journal of Biology
To create new varieties resistant to biotic and abiotic stress factors that possess high productivity and improved fruit quality, breeders require modern breeding methods and techniques. Double haploid (DH) lines are an invaluable breeding element due to their guaranteed, completely homozygous nature. One of the fastest methods for obtaining DH lines is anther culture. Microspore embryogenesis provides considerable opportunities in the areas of breeding and biotechnology and represents a model system for fundamental biological research. The effectiveness of pepper anther culture is still low, which affects the application of DH lines in breeding programs. A detailed study of the anther …
Enhanced Salt Tolerance Of Transgenic Tobacco Expressing A Wheat Salt Tolerance Gene, Musa Kavas, Mehmet Cengi̇z Baloğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem
Enhanced Salt Tolerance Of Transgenic Tobacco Expressing A Wheat Salt Tolerance Gene, Musa Kavas, Mehmet Cengi̇z Baloğlu, Ayşe Meral Yücel, Hüseyi̇n Avni̇ Öktem
Turkish Journal of Biology
Soil salinity is one of the most important limiting factors of agricultural productivity in the world. The Triticum aestivum salt tolerance-related gene (TaSTRG) possesses a functional response to salt and drought stress conditions. A variety of stress factors, such as salt, drought, abscisic acid, and cold, may induce the expression of TaSTRG in wheat. In this study, the TaSTRG gene was transferred to tobacco via Agrobacterium-mediated transformation. Overexpression of TaSTRG in transgenic tobacco plants indicated higher salt tolerance and mediated more vigorous growth than in wild-type plants. Under salt stress conditions, the transgenic tobacco plants had higher germination and survival …
A New Reliable And Sensitive Nested Pcr Assay Based On The Human Sry Gene For Detection Of Interspecific Chimeras, Jie Yu, Kaijing Li, Main Huang, Liping Guan, Min Zhang, Weihua Li, Jianfa Huang, Ting Luo, Wencong Wang, Bikun Xian, Xing Liu, Bing Huang
A New Reliable And Sensitive Nested Pcr Assay Based On The Human Sry Gene For Detection Of Interspecific Chimeras, Jie Yu, Kaijing Li, Main Huang, Liping Guan, Min Zhang, Weihua Li, Jianfa Huang, Ting Luo, Wencong Wang, Bikun Xian, Xing Liu, Bing Huang
Turkish Journal of Biology
Although interspecific chimeras can be identified using laborious techniques, more accurate and rapid detectable methods need to be established. The present study develops a nested polymerase chain reaction (nPCR) assay to detect human-mouse chimeras. A set of previously validated outer primers, specific to the human SRY gene, was used for conventional one-step PCR, while the inner primers for nPCR were designed. The specificities of PCR and nPCR assays were examined; both primer sets yielded PCR amplification products from male human epidermis-derived mesenchymal stem cell-like pluripotent cell DNA but no amplification products from negative control DNA. The sensitivity of this nPCR …
Agrobacterium-Mediated Transformation Of Five Inbred Maize Lines With The Brittle 2 Gene, Thuy Ninh Nguyen, Thi Luong Tran, Duc Thanh Nguyen
Agrobacterium-Mediated Transformation Of Five Inbred Maize Lines With The Brittle 2 Gene, Thuy Ninh Nguyen, Thi Luong Tran, Duc Thanh Nguyen
Turkish Journal of Biology
The Brittle 2 (Bt2) gene encodes a small subunit of ADP-glucose pyrophosphorylase (AGPase) enzyme, one of the enzymes that play an important role in endosperm starch biosynthesis. We carried out a study to transfer the Bt2 gene into several inbred maize lines and regenerate transgenic maize plants possessing the transgene, which can be used to further study the role of the Bt2 gene in starch biosynthesis. Agrobacterium tumefaciens strains C58 and EHA105 harboring the transformation vector pCambia2300/Ubi-Bt2-Nos were used to transfer the Bt2 gene into five inbred maize lines, H14, H26, H240, H95, and N18. Transformed calli were selected on …
Pluronic Pf68 Increases Transfection Efficiency In Electroporationof Mesenchymal Stem Cells, Safa Aydin, Mehmet Emi̇r Yalvaç, Ferruh Özcan, Fi̇kretti̇n Şahi̇n
Pluronic Pf68 Increases Transfection Efficiency In Electroporationof Mesenchymal Stem Cells, Safa Aydin, Mehmet Emi̇r Yalvaç, Ferruh Özcan, Fi̇kretti̇n Şahi̇n
Turkish Journal of Biology
Electroporation is a safe and efficient method to transfect human tooth germ stem cells (hTGSCs), which are mainly mesenchymal stem cells (MSCs). In this study, pluronic F68 (PF68), a nonionic and low-foaming surfactant, was used for the first time to increase the transfection efficiency in electroporation of hTGSCs. Our results revealed that when PF68 was used in the preincubation, electroporation, and recovery medium, it dramatically increased transfection efficiency. Moreover, use of PF68 did not alter the differentiation capacity of hTGSCs, whereas cells electroporated without PF68 had significantly lower expression of Oct-4, pluripotency marker gene, and alkaline phosphatase (ALP) activity upon …
Lycium Barbarum L. (Goji Berry) Fruits Improve Anxiety, Depression-Like Behaviors, And Learning Performance: The Moderating Role Of Sex, Fatma Pehli̇van Karakaş, Hami̇t Coşkun, Kadi̇r Sağlam, Bi̇hter Gökçe Bozat
Lycium Barbarum L. (Goji Berry) Fruits Improve Anxiety, Depression-Like Behaviors, And Learning Performance: The Moderating Role Of Sex, Fatma Pehli̇van Karakaş, Hami̇t Coşkun, Kadi̇r Sağlam, Bi̇hter Gökçe Bozat
Turkish Journal of Biology
The aim of this research was to investigate the effects of the methanol extract obtained from Lycium barbarum fruits on anxiety, depression-like behaviors, and spatial memory in Wistar albino rats. A total of 28 rats were selected, randomly assigned to four experimental groups, and tested by means of the open field and elevated plus maze tests for anxiety-like behaviors, the forced swim test for depression-like behaviors, and the Morris water maze test for spatial memory. The findings demonstrated that in the open field, L. Barbarum-administered rats spent more time at the center and showed more mobility and velocity than controls. …
Purification And Properties Of Lipoxygenase From Opium Poppy Seedlings(Papaver Somniferum L.), Ivana Holková, Frantisek Bilka, Drahomíra Rauová, Lydia Bezáková
Purification And Properties Of Lipoxygenase From Opium Poppy Seedlings(Papaver Somniferum L.), Ivana Holková, Frantisek Bilka, Drahomíra Rauová, Lydia Bezáková
Turkish Journal of Biology
Lipoxygenase (linoleate:oxygen oxidoreductase; EC 1.13.11.12; LOX) is a key enzyme in the signaling pathway leading to the production of biologically active compounds involved in plant growth development and stress responses. In this study the novel LOX enzyme from opium poppy (Papaver somniferum L.) seedlings was purified to electrophoretic homogeneity and characterized. LOX activity during the germination of opium poppy seeds was analyzed, and the highest LOX activity was determined on the fourth day of germination. Opium poppy LOX was purified from 4-day-old poppy seedlings after ammonium sulfate precipitation followed by hydrophobic chromatography (Phenyl-Sepharose CL-4B), ion exchange chromatography (Q-Sepharose), and affinity …
Role Of Glucose Repression In The Oxidative Stress Response Ofschizosaccharomyces Pombe: Analysis Of Transcript Levels Of Fbp1, Hxk2, Sod1and Ctt1 Genes In Sty1, Atf1 And Pap1 Knock-Out Mutants, Bedi̇a Palabiyik, Farinaz Jafari Ghods, Semi̇an Karaer Uzuner
Role Of Glucose Repression In The Oxidative Stress Response Ofschizosaccharomyces Pombe: Analysis Of Transcript Levels Of Fbp1, Hxk2, Sod1and Ctt1 Genes In Sty1, Atf1 And Pap1 Knock-Out Mutants, Bedi̇a Palabiyik, Farinaz Jafari Ghods, Semi̇an Karaer Uzuner
Turkish Journal of Biology
Previously we reported that the glucose sensing/signaling pathway affected the oxidative stress response of Schizosaccharomyces pombe, based on studies of glucose-repression and oxidative-stress-resistant mutants including ird5. sty1, encoding a protein kinase, and aft1 and pap1, encoding transcription factors, are important components of the oxidative stress response in S. Pombe. To analyze the relationship between the glucose sensing/signaling pathway and the oxidative stress response, we generated sty1, aft1, and pap1 knock-out mutants in ird5 and wild-type backgrounds. We evaluated the survival rates of the ird5 double mutants (5A, 5P, and 5S) and wild-type single mutants (9A, 9P, and 9S) under mild …
The Effects Of Dna Methyl Transferases On Antiaging Klotho Gene Expression, Eli̇f Çağlayan, Kadi̇r Turan
The Effects Of Dna Methyl Transferases On Antiaging Klotho Gene Expression, Eli̇f Çağlayan, Kadi̇r Turan
Turkish Journal of Biology
Several intracellular metabolic pathways in which multiple genes have a role affect the aging process. One important genetic factor associated with aging is klotho gene. In some recently published studies, klotho gene has also been associated with several types of cancer and identified as a tumor suppressor gene. Therefore, elucidation of the control of klotho gene expression has become more important. Results suggesting that the promoter region of human klotho gene could be epigenetically controlled by DNA methylation have been reported. In contrast, a study revealing whether a change in the expression level of DNMT enzymes in the cells affects …