Life Sciences Commons^™

Definition Of The Landscape Of Chromatin Structure At The Frataxin Gene In Friedreich’S Ataxia, Eunah Kim

Dissertations and Theses (Open Access)

Friedreich’s ataxia (FRDA) is caused by the transcriptional silencing of the frataxin (FXN) gene. FRDA patients have expansion of GAA repeats in intron 1 of the FXN gene in both alleles. A number of studies demonstrated that specific histone deacetylase inhibitors (HDACi) affect either histone modifications at the FXN gene or FXN expression in FRDA cells, indicating that the hyperexpanded GAA repeat may facilitate heterochromatin formation. However, the correlation between chromatin structure and transcription at the FXN gene is currently limited due to a lack of more detailed analysis. Therefore, I analyzed the effects of the hyperexpanded GAA …

The Role Of E2f1 In The Response To Dna Double Strand Breaks, Jie Chen

Dissertations and Theses (Open Access)

The importance of E2F transcription factors in the processes of proliferation and apoptosis are well established. E2F1, but not other E2F family members, is also phosphorylated and stabilized in response to various forms of DNA damage to regulate the expression of cell cycle and pro-apoptotic genes. E2F1 also relocalizes and forms foci at sites of DNA double-strand breaks but the function of E2F1 at sites of damage is still unknown. Here I reveal that E2F1 deficiency leads to increased spontaneous DNA break and impaired recovery following exposure to ionizing radiation. In response to DNA double-strand breaks, NBS1 phosphorylation and foci …

The Dissociation Of Location And Object Working Memory Using Fmri And Meg, Antony Passaro

Dissertations and Theses (Open Access)

Visual working memory (VWM) involves maintaining and processing visual information, often for the purpose of making immediate decisions. Neuroimaging experiments of VWM provide evidence in support of a neural system mainly involving a fronto-parietal neuronal network, but the role of specific brain areas is less clear. A proposal that has recently generated considerable debate suggests that a dissociation of object and location VWM occurs within the prefrontal cortex, in dorsal and ventral regions, respectively. However, re-examination of the relevant literature presents a more robust distribution suggestive of a general caudal-rostral dissociation from occipital and parietal structures, caudally, to prefrontal regions, …

Role Of The Gcn5 Histone Acetyltransferase In Spinocerebellar Ataxia Type 7 And In Immature Neurons, Yi Chun Chen

Dissertations and Theses (Open Access)

Spinocerebellar Ataxia type 7 (SCA7) is a neurodegenerative disease caused by expansion of a CAG repeat encoding a polyglutamine tract in ATXN7, a component of the SAGA histone acetyltransferase (HAT) complex. Previous studies provided conflicting evidence regarding the effects of polyQ-ATXN7 on the activity of Gcn5, the HAT catalytic subunit of SAGA. Here I showed that reducing Gcn5 expression accelerates both cerebellar and retinal degeneration in a mouse model of SCA7. Deletion of Gcn5 in Purkinje cells in mice expressing wild type Atxn7, however, causes only mild ataxia and does not lead to the early lethality observed in SCA7 mice. …

The Cytoplasmic Tail Of Mhc Class I Molecules Plays A Critical Role In Dendritic Cell-Induced T Cell Immunity, Tania G. Rodriguez-Cruz

Dissertations and Theses (Open Access)

The presentation of MHC class I (MHC-I)/peptide complexes by dendritic cells (DCs) is critical for the maintenance of central tolerance to self and for the regulation of cytotoxic T lymphocytes (CTL)-mediated adaptive immune responses against pathogens and cancer cells. Interestingly, several findings have suggested that the cytoplasmic tail of MHC class I plays a functional role in the regulation of CTL immune responses. For example, our previous studies demonstrated that exon 7-deleted MHC-I molecules not only showed extended DC cell surface half-lives but also induced significantly increased CTL responses to viral challange invivo. Although exon 7-deleted variant of MHC-I does …

Review Of Sandhill And Whooping Cranes: Ancient Voices Over America's Wetlands By Paul Johnsgard, Jane E. Austin

The Prairie Naturalist

Paul Johnsgard has long been captivated by wild cranes and their unique vocalizations, courtship dances, and wide-ranging migrations. As a scientist and an admirer, Johnsgard has watched their migrations and behaviors for decades as hundreds of thousands of cranes staged each spring by the central Platte River, not far from his home in Lincoln, Nebraska. As an artist, he has skillfully captured their courtship dances and other behaviors in his exceptional line drawings. And, as an author, he has written extensively on their ecology in three earlier books: Cranes of the World (1983), Those of the Gray Wind: The Sandhill …

Book Review: Effects Of Climate Change On Birds, Edited By Anders Pape Meller, Wolfgang Fiedler, And Peter Berthold, Brian J. Olsen

The Prairie Naturalist

Book review of Effects of Climate Change on Birds, edited by Anders Pape Meller, Wolfgang Fiedler, and Peter Berthold.

Climate scientists from across the globe predict vast changes during the next century in the planet's temperatures, precipitation, storm intensities, fire regimes, hydrologic cycles, and atmospheric, water, and soil chemistries. The changes will be global, but their effects will be felt locally everywhere. To find a scientifically and socially engaging bellwether of these events, we need to look only through the lens of avian biology. Birds are found from pole to pole; their movements connect continents. They are loud, colorful …

Dna Electrophoretic Migration Patterns Change After Exposure Of Jurkat Cells To A Single Intense Nanosecond Electric Pulse, Stefania Romeo, Luigi Zeni, Maurizio Sarti, Anna Sannino, Maria Rosaria Scarfi, P. Thomas Vernier, Olga Zeni

Bioelectrics Publications

Intense nanosecond pulsed electric fields (nsPEFs) interact with cellular membranes and intracellular structures. Investigating how cells respond to nanosecond pulses is essential for a) development of biomedical applications of nsPEFs, including cancer therapy, and b) better understanding of the mechanisms underlying such bioelectrical effects. In this work, we explored relatively mild exposure conditions to provide insight into weak, reversible effects, laying a foundation for a better understanding of the interaction mechanisms and kinetics underlying nsPEF bio-effects. In particular, we report changes in the nucleus of Jurkat cells (human lymphoblastoid T cells) exposed to single pulses of 60 ns duration and …

Relative Importance Of Environmental Variables For Spawning Cues And Tributary Use By An Adfluvial Lake Sucker, Brian A. Hines

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The endangered June sucker (Chasmistes liorus mictus), which is only found in Utah Lake, UT, historically spawned in all streams flowing into the lake, but due to human-caused changes their spawning is restricted to the Provo River. The purpose of this study was to gain a better understanding of the spawning and early life-history of the
June sucker for recovery purposes. My specific objectives were to determine:

1. what environmental factors attract or deter June suckers to certain Utah Lake tributaries for spawning,
2. what cues June suckers to migrate upstream to spawn,
3. if June suckers use more than one …

Effects Of Tannin-Containing Feed On Haemonchous Contortus In Sheep And Its Behavioral Implications, Jessica A. Juhnke

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Herbivores prefer foods that supply required nutrients and avoid those with excess nutrients and plant secondary compounds (PSC). Nevertheless, PSC such as condensed tannins can provide beneficial medicinal effects to herbivores. The objectives of this study were to determine: 1) if parasitized lambs increased preference for a tannin-rich food after they experienced the beneficial antiparasitic effects of tannins relative to parasitized lambs that did not experience such benefits, 2) if preference for the tannin-containing food in the former group decreased when parasite burdens subsided, and 3) if the tannin-enriched food decreased parasitic burdens.

Twenty-two lambs were familiarized with beet pulp …

Molecular Interactions Of Salmonella With The Host Epithelium In Presence Of Commensals, Prerak T. Desai

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Food-borne infections are a major source of mortality and morbidity. Salmonella causes the highest number of Food-borne bacterial infections in the US. This work contributes towards developing strategies to control Salmonella by (a) defining receptors used by Salmonella to adhere to and invade the host epithelium; (b) developing a host receptor based rapid detection method for the pathogen in food matrix; (C) and defining mechanisms of how probiotics can help alleviate Salmonella-induced cell death in the host epithelium.

We developed a cell-cell crosslinking method to discover host-microbe receptors, and discovered three new receptor-ligand interactions. Interaction of Salmonella Ef-Tu with …

Have Introduced Brown Trout (Salmo Trutta) Affected Native Aquatic Vertebrates In Western United States Streams?, Nora K. Burbank

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The introduction of exotic species to areas outside of their native range is one of the greatest threats to the biodiversity of native freshwater organisms. Exotic species have outcompeted native species for resources such as food and shelter, and in some cases these interactions have resulted in local extinctions or reductions in abundance of those native species. Brown trout are native to Eurasia, but have been introduced to much of the rest of the world, including the United States. In some parts of their introduced range, brown trout have substantially reduced the abundance and home ranges of some native fish …

Characterizing Water And Nitrogen Dynamics In Urban/Suburban Landscapes, Hongyan Sun

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This research investigated the water use of different plant types in urban landscapes, nitrogen (N) and water transport in turf, and potential N leaching from urban landscapes to ground water. In the first study, three landscape treatments integrating different types of plants—woody, herbaceous perennial, turf—and putative water use classifications—Mesic, Mixed, Xeric—were grown in large drainage lysimeters. Each landscape plot was divided into woody, turf, and herbaceous perennial plant hydrozones and irrigated for optimum water status over two years, with water use measured using a water balance approach. For woody plants and herbaceous perennials, canopy cover, rather than plant type or …

Isotopic Tracer Reveals Depth-Specific Water Use Patterns Between Two Adjacent Native And Non-Native Plant Communities, Clémence Pascale Warren

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Non-native plants have invaded over 100 millions of acres of western arid land in the US and dramatically altered nutrient cycling rates. Changes in water cycling caused by invasive species are of particular interest because primary production in the Western US is typically limited by water availability and aquifer recharge reflects plant demand. Large-scale invasions can, therefore, be expected to cause large-scale changes in hydrological cycles, but until recently, there have been considerable limitations in the ability to measure the timing, location, and extent of water use. Here we injected a tracer, deuterated water (D₂O), into five soil …

An Assessment Of Vegetation Metrics And Plot Types To Measure Seasonal Variation And Grazing Effects On Riparian Plant Communities, Caroline M. Laine

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Monitoring changes in vegetation is important on public lands. Observing how plant communities respond to changes in land management can give insightful information about which management practices are sustainable. Because water is scarce in the western United States, many land managers focus their monitoring efforts along streams. However, monitoring streamside vegetation is challenging due to a variety of factors and can be confounded by factors such as seasonal climate variation and management activities, like cattle grazing.

In vegetation assessment, there are a variety of measurements that are taken to glean valuable information. Some attributes of interest include how much vegetation …

Analysis Of Food Web Effects Of Non-Native Fishes And Evaluation Of Stream Restoration Potential For The San Rafael River, Utah, Timothy E. Walsworth

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Native fishes of the Colorado River Basin have experienced dramatic reductions in range and abundance as a result of extensive human alterations to the basin’s waterways. Many of these native fishes are federally listed under the Endangered Species Act, while several others are subject to range-wide conservation agreements between state and federal management agencies. Three of the native species subject to range-wide conservation agreements are the flannelmouth sucker, bluehead sucker, and roundtail chub (hereafter, the “three species”). Each of the “three species” is still found in the San Rafael River of southeastern Utah, which has experienced habitat degradation and non-native …

Assessment Of Colorado’S Wilderness Areas: Manager Perceptions And Remoteness Modeling, Gary D. Vaughn

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

This study assessed visitor use levels and resource and social conditions in wilderness areas across the State of Colorado using existing and collected spatial data. This is the first attempt to spatially assess wilderness conditions at the state level. A state-wide assessment of wilderness conditions allows local and regional managers to make informed regional decisions and to prioritize and direct their time and energy efficiently. This assessment clarifies the recreational use and impacts across the state. This study consists of two projects: 1) managers’ perceptions of the location and extent of resource and social condition problems; and 2) a geographic …

Using Species Distribution Models To Assess Invasion Theory And Provide Management Recommendations For Riparian Areas In The Eastern Columbia And Western Missouri River Basins, Diane R. Menuz

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Globalization has created opportunities for plant and animal species to be transported to novel ecosystems. A subset of these species become invasive species, which are able to persist and spread rapidly to become influential components in their new ecosystems. Invasive species are often able to out-compete co-occurring native species and can alter fundamental ecosystem properties, such as soil nutrient availability or time between wildfires in a region. The United States is estimated to lose $34 billion per year to invasive plant species through costs to control unwanted species and lowered crop and forage production.

We used statistical models to better …

Reconstructing The Holocene Arroyo History Of The Upper Escalante River, Southern Utah, Using Optically Stimulated Luminescence (Osl) And Radiocarbon Dating, Anne E. Hayden

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Arroyos are steep-walled, entrenched, typically ephemeral streams commonly found in dryland river systems that form when streams incise into previously deposited alluvial fill. Arroyos in the southwestern U.S. have been studied extensively following the historic period of arroyo cutting in the late 1800s and early 1900s A.D. The upper Escalante River in south-central Utah similarly began incising in 1909, and records evidence for past cut and fill cycles in well-exposed walls along the now continuous arroyo.

Establishing robust geochronologies of past arroyo cycles in these fluvial settings has been difficult. Recent improvements in accelerator mass spectrometry (AMS) radiocarbon (^{14 …}

Rotating Algal Biofilm Reactors: Mathematical Modeling And Lipid Production, Paul A. Woolsey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Harvesting of algal biomass presents a large barrier to the success of biofuels made from algae feedstock. Small cell sizes coupled with dilute concentrations of biomass in lagoon systems make separation an expensive and energy intense-process. The rotating algal biofilm reactor (RABR) has been developed at USU to provide a sustainable technology solution to this issue. Algae cells grown as a biofilm are concentrated in one location for ease of harvesting of high density biomass. A mathematical model of this biofilm system was developed based on data generated from three pilot scale reactors at the City of Logan, Utah wastewater …

Causes And Consequences Of Plant Spatial Patterns On Natural And Experimental Great Basin (Usa) Plant Communities, Andrew P. Rayburn

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The processes by which plant spatial patterns are formed, and the effects of those patterns on plant community dynamics, remain important areas of research in plant ecology. Plant spatial pattern formation has been linked to many ecological processes that act to structure plant communities at different spatiotemporal scales. Past studies of pattern formation are common, but recent methodological advances in data collection and analysis have permitted researchers to conduct more advanced observational studies of pattern formation in space and time. While studies of the effects of plant spatial patterns were formally rare, they have increased in the last decade as …

Front Matter

The Synapse: Intercollegiate science magazine

No abstract provided.

Alohamora! Unlocking Magic With The Wonders Of Science, Anna Dardick

The Synapse: Intercollegiate science magazine

No abstract provided.

What's Up, Weelic?: Tracking Neural Pathways With A Sugar-Coated Virus, Weelic Chong

The Synapse: Intercollegiate science magazine

No abstract provided.

This Is Your Brain On Acetylcholine, Ksenia Vlasov

The Synapse: Intercollegiate science magazine

No abstract provided.

What Are Friends For?: The Importance Of Friend Groups In Academic Success, Emily Neuhoff

The Synapse: Intercollegiate science magazine

No abstract provided.

Mike Got A Bigger Bike: Mike Rauscher On Nuclear Space Travel, Quinton Steele

The Synapse: Intercollegiate science magazine

No abstract provided.

Honors Research, Matt Hartsock, Sam Asinof, Sage Aronson, Yuhua Lu

The Synapse: Intercollegiate science magazine

No abstract provided.

Fatty Acids And Lipogenesis In Ruminant Adipocytes, Taber Burns

All Dissertations

Obsesity, the excess deposition of white adipose tissue, is a growing problem in the U.S. and other developed countries. Formerly thought to be inert, adipose tissue is now recognized as a dynamic endocrine organ with its secretion of adipokines and a newly proposed class hormone class 'lipokine'. Adipocytes are the functional unit of adipose tissue and can influence the tissue through hyperplasic and hypertrophic growth. In order to investigate the mechanisms involved in adipogenesis and lipogenesis of adipose tissue, stromal vascular cultures were isolated from adipose tissue of finishing cattle for use in experiments.
There is a positive relationship between …

Thermal Inactivation Of Stress Adapted Pathogens In Compost, Randhir Singh

All Dissertations

In the United States, 1.3 billion tons of animal wastes are produced annually. Disposal of this huge amount of waste on agricultural land without proper treatment is a public health safety issue as animal waste is a potential source of several human pathogens. Therefore, composting of animal wastes is an economical solution to this problem. The high temperature reached during this process also brings about inactivation of pathogens in the waste. However, survival of pathogens has still been reported from different composting studies, indicating the complex nature of this process. The objectives of this study were to: 1) study thermal …