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2011

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Articles 361 - 390 of 5491

Full-Text Articles in Life Sciences

Determining The Validity Of The Nintendo Wii Balance Board As An Assessment Tool For Balance, Sabrina Mae Deans Dec 2011

Determining The Validity Of The Nintendo Wii Balance Board As An Assessment Tool For Balance, Sabrina Mae Deans

UNLV Theses, Dissertations, Professional Papers, and Capstones

Context. Application of the Nintendo Wii-fit balance board and its games have been used in Physical Therapy clinics, showing success in individuals with neurological disorders, and has been recommended as a minimum baseline assessment of a symptoms checklist and standardized cognitive and balance assessments for concussion management by the NCAA. However, it still faces challenges of being considered a reliable and consistent tool for producing normative data in the allied healthcare. Because there is little to no evidence for the Wii-fit balance board as a valid balance assessment tool for clinical and/or research usage, the significance of this study is …


A Polymerase Chain Reaction Method For The Detection Of Selenomonas Noxia, Arthuro Mehretu Dec 2011

A Polymerase Chain Reaction Method For The Detection Of Selenomonas Noxia, Arthuro Mehretu

UNLV Theses, Dissertations, Professional Papers, and Capstones

In recent studies, periodontal health has been linked to being overweight and/or obese. Among common oral bacteria, Selenomonas noxia has been implicated in converting periodontal health to disease. Selenomonas spp. have also been found in gastric ulcers, and were misdiagnosed as Helicobacter -like organisms, but no further studies were conducted. The increasing clinical and epidemiological importance of S. noxia necessitates the development of a rapid detection method. In this study, a TaqMan 16S rRNA based real-time Polymerase Chain Reaction (PCR) method was developed, optimized and evaluated for the rapid and specific detection of S. noxia . The 16S PCR assay …


Retrospective Evaluation Of Microbial Presence In Existing Saliva Repository: A Pcr Based Molecular Survey Of Oral Microbial Populations From Existing Saliva Samples, Jay Ericksen Davis Dec 2011

Retrospective Evaluation Of Microbial Presence In Existing Saliva Repository: A Pcr Based Molecular Survey Of Oral Microbial Populations From Existing Saliva Samples, Jay Ericksen Davis

UNLV Theses, Dissertations, Professional Papers, and Capstones

The overall purpose of this research project is to explore the link between oral health and disease in the University of Nevada, Las Vegas - School of Dental Medicine (UNLV-SDM) clinic patient population. More specifically, the population of interest is the UNLV-SDM orthodontic clinic patient population, which is mostly composed of adults, females, and minorities. The University of Nevada, Las Vegas, School of Dental Medicine, with more than 70,000 active patients and a post-graduate specialty program in Orthodontics is uniquely positioned to perform this type of oral health epidemiology survey, as well as targeted oral microbial testing among populations within …


A Landscape Approach To Late Prehistoric Settlement And Subsistence Patterns In The Mojave Sink, Tiffany Ann Thomas Dec 2011

A Landscape Approach To Late Prehistoric Settlement And Subsistence Patterns In The Mojave Sink, Tiffany Ann Thomas

UNLV Theses, Dissertations, Professional Papers, and Capstones

The environment of the Late Prehistoric period (1200 A.D. to Historic Contact) Mojave Sink was wetter than modern conditions. The settlement and subsistence patterns of the occupants of the region during this period were driven by the availability of water, subsistence resources, raw material sources, and tradition. These people utilized the regional landscape based upon the seasonal availability of these resources. Supplemental agricultural production has been proposed for the Mojave River Delta due to the more favorable environmental conditions of this period. If agriculture was being practiced it would have affected the regional land-use patterns. For this thesis I propose …


Assessing Biodegradable Containers For Growing Long-Term Greenhouse Crops In Subirrigation System, Stephanie A. Beeks Dec 2011

Assessing Biodegradable Containers For Growing Long-Term Greenhouse Crops In Subirrigation System, Stephanie A. Beeks

Graduate Theses and Dissertations

The objective for this research was to assess the efficacy of biodegradable containers compared to plastic containers for the production of long-term crops using a subirrigation system. Plastic and various biocontainers were used to grow `Rainier Purple' cyclamen (Cyclamen persicum L.) in ebb-and-flood benches. After 15 weeks, the physical properties of the containers were tested. The used plastic containers had strengths of 23.3, 23.5 and 21.9 g for the bottom, middle and top zones respectively. The peat, dairy manure and wood fiber containers had strengths of less than 2 kg for each zone. The peat, dairy manure, wood fiber and …


Bycatch Of The Lake Pontchartrain Basin Inshore Shrimp Fishery And Its Effect On Two Sea Catfish Species: The Gafftopsail Catfish (Bagre Marinus) And The Hardhead Catfish (Ariopsis Felis), Scott P. Eustis Dec 2011

Bycatch Of The Lake Pontchartrain Basin Inshore Shrimp Fishery And Its Effect On Two Sea Catfish Species: The Gafftopsail Catfish (Bagre Marinus) And The Hardhead Catfish (Ariopsis Felis), Scott P. Eustis

LSU New Orleans Theses and Dissertations

In Lake Pontchartrain Basin, commercial fishing in estuarine habitats impacts many non-target species collected as bycatch. I investigated the bycatch assemblages collected by commercial vessels and compared these to assemblages collected by typical fishery-independent methods. I compared assemblages using analysis of similarity (ANOSIM) and determined important species by weight and abundance using similarity percentages analyses (SIMPER). I also examined differences in size-class distributions by gear type using density kernel plots and Mann-Whitney U tests. The two gear types collected significantly different assemblages (ANOSIM R = 0.522, p = 0.001) and gear type explained more composition differences than other factors such …


Bryoecology In The American Southwest: Patterns Of Biodiversity And Responses To Global Change, John Carroll Brinda Dec 2011

Bryoecology In The American Southwest: Patterns Of Biodiversity And Responses To Global Change, John Carroll Brinda

UNLV Theses, Dissertations, Professional Papers, and Capstones

This dissertation combines investigation of the large-scale responses of bryophyte species diversity and distribution with small-scale physiological adaptations to global change. These two areas of inquiry are linked because one way to predict plant species responses to global change is to examine their distribution across current ecological gradients produced by factors such as latitude and elevation. By examining these biogeographic patterns one can identify those species that have a narrow tolerance and therefore are most sensitive to change. Selected bryophytes might then be used as indicator species in long-term monitoring programs. Where historical data exist, these can be used to …


Effect Of A Work Site Exercise Program On Selected Fitness And Psychological Parameters, Kristen Ann Christiansen Dec 2011

Effect Of A Work Site Exercise Program On Selected Fitness And Psychological Parameters, Kristen Ann Christiansen

UNLV Theses, Dissertations, Professional Papers, and Capstones

The increasing rates of obesity continue to threaten the vitality of our nation. Health care costs are soaring and chronic diseases are reaching even the youngest populations. Physical activity is an integral component in reversing the obesity epidemic and improving the health of today's workforce. The present study sought to determine the effect of a work site exercise program on select fitness and psychological parameters. Five female employees participated in a six-week exercise program utilizing HealthBeat(TM) outdoor exercise equipment in a circuit fashion for 30 minutes, 2 days per week. Eighty percent of participants were categorized as sedentary or low-active …


Global And Specific Controls Of Protein Synthesis In Hibernators, Peipei Pan Dec 2011

Global And Specific Controls Of Protein Synthesis In Hibernators, Peipei Pan

UNLV Theses, Dissertations, Professional Papers, and Capstones

Mammalian hibernation is a highly dynamic physiological process that is composed of a series of torpor bouts, wherein hibernators oscillate between periods of torpor and interbout arousal. Although normally vital to homeostasis, many energetically consumptive processes such as translation or protein synthesis are virtually ceased during hibernation. Earlier studies indicated that protein synthesis had fallen to almost negligible levels. Cap-dependent initiation of translation is well regulated by eukaryotic translation initiation factor 4E (eIF4E) and its binding partner eIF4E-binding protein 1 (4E-BP1) when hibernators cycle in and out the torpor state. Herein, I investigated well-characterized regulatory mechanisms of global and specific …


Microbial Impacts On Endocrine Disrupting Contaminants: Las Vegas Wash And Lake Mead, Nevada, Susanna May Blunt Dec 2011

Microbial Impacts On Endocrine Disrupting Contaminants: Las Vegas Wash And Lake Mead, Nevada, Susanna May Blunt

UNLV Theses, Dissertations, Professional Papers, and Capstones

International concern over endocrine disrupting chemicals (EDCs) has become heightened in recent years as more studies reveal their persistence in the environment and their detrimental effects on wildlife. However, little is known about the role of microorganisms in the fate and transport of these compounds in surface waters. Las Vegas Wash, a stream flowing into Lake Mead and fed primarily by treated wastewater effluent, provided a unique experimental system in which to study the role microorganisms play in the dispersal of these compounds in aquatic systems. Samples were collected from the Las Vegas Wash downstream of the Las Vegas Valley's …


A Population-Genetic Perspective On The Similarities And Differences Among Worldwide Human Populations, Noah A. Rosenberg Dec 2011

A Population-Genetic Perspective On The Similarities And Differences Among Worldwide Human Populations, Noah A. Rosenberg

Human Biology Open Access Pre-Prints

Recent studies have produced a variety of advances in the investigation of genetic similarities and differences among human populations. Here, I pose a series of questions about human population- genetic similarities and differences, and I then answer these questions by numerical computation with a single shared population-genetic dataset. The collection of answers obtained provides an introductory perspective for understanding key results on the features of worldwide human genetic variation.


Effect Of Diet Quantity And Quality On Female Sampling Behavior And Mating Preferences In A Field Cricket, Heidi L. Bulfer Dec 2011

Effect Of Diet Quantity And Quality On Female Sampling Behavior And Mating Preferences In A Field Cricket, Heidi L. Bulfer

School of Biological Sciences: Dissertations, Theses, and Student Research

Understanding the adaptive significance of variation in female mating behaviour is important because variation may often be favored by selection instead of a change in mean mating behaviour, particularly in variable environments. Females are known to adjust their mating behaviour to a variety of intrinsic and extrinsic factors including nutrition. There are multiple reasons why female behaviours might vary with nutrition; we tested two of these hypotheses: the search cost hypothesis and the direct benefits hypothesis. These hypotheses are not mutually exclusive, but they make contrasting predictions under some conditions. Low nutrition females may have less available energy to support …


Life History Tradeoffs And Phenotypic Plasticity: The Tale Of A Flight Polyphenic Field Cricket, Chandreyee Mitra Dec 2011

Life History Tradeoffs And Phenotypic Plasticity: The Tale Of A Flight Polyphenic Field Cricket, Chandreyee Mitra

School of Biological Sciences: Dissertations, Theses, and Student Research

Most organisms are resource limited. Such limitations can result in tradeoffs between life history traits -- any traits that affect survival or reproduction. Flight polyphenic field crickets are thought to be a classic example of such a life history tradeoff, in which individuals tradeoff investment in flight capability and investment in reproduction. This polyphenism results from the interaction of two morphological traits: wing morphology (short or long) and flight muscle morphology (functional pink or non-functional white), and is affected by both genes and the environment. I examined life history traits of a flight polyphenic field cricket, Gryllus lineaticeps. First, …


Analysis Of Turnip Crinkle Virus Effects On The Innate And Adaptive Immunity In Arabidopsis Thaliana, Teresa J. Donze Dec 2011

Analysis Of Turnip Crinkle Virus Effects On The Innate And Adaptive Immunity In Arabidopsis Thaliana, Teresa J. Donze

School of Biological Sciences: Dissertations, Theses, and Student Research

The ability to understand the interactions between plants and the variety of pathogens they encounter on a daily basis is an important area of research. In the following work presented in this dissertation, I sought to better understand the mechanisms that Turnip crinkle virus (TCV) employs to elude the defense responses of the host plant Arabidopsis thaliana. It was previously determined that TCV coat protein (CP) interacts with a transcription factor, TIP, within the about 10 amino acid region near the N-terminus of the CP called the R-domain. When this interaction was disrupted by making single amino acid substitutions …


Msh1 Influence On Plant Mitochondrial Genome Recombination And Phenotype In Tobacco, Peibei Sun Dec 2011

Msh1 Influence On Plant Mitochondrial Genome Recombination And Phenotype In Tobacco, Peibei Sun

School of Biological Sciences: Dissertations, Theses, and Student Research

Plant mitochondrial genomes are composed of unusually complex structures, due to active recombination at numerous repeated sequences in the genome. The maintenance of mitochondrial genome stability is under the control of identifiable nuclear genes. In plants, three nuclear genes (MSH1, RECA3 and OSB1) have been shown to participate in recombination surveillance and the suppression of illegitimate recombination in mitochondria. Disruption of these loci in Arabidopsis results in reproducible mitochondrial genome rearrangements. We demonstrated that repeat-mediated de novo recombination was also enhanced in both Arabidopsis and tobacco during passage through in vitro culture. Furthermore, in vitro conditions led …


Impact Of Crop And Residue Management On The Physical And Chemical Stabilization Of Soil Organic Matter At Farm Level, Ana B. Wingeyer Dec 2011

Impact Of Crop And Residue Management On The Physical And Chemical Stabilization Of Soil Organic Matter At Farm Level, Ana B. Wingeyer

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

This dissertation explores changes over time in soil organic matter (SOM) stabilization of two irrigated production fields: a continuous maize sequence that was converted from no-till to conservation deep tillage (Site 1), and a no-till maize-soybean rotation (Site 2). An integrated approach using humic acid extractions and density-based physical fractionation of SOM within aggregate size classes was developed to evaluate the changes in SOM stabilization (physical protection, organo-mineral associations and humification). At Site 1, loss of SOM in the surface layer was compensated for by increased SOM in deeper soil layers with no net change in C stocks. Whole field …


Review Of Teaching Children Science: Hands-On Nature Study In North America, 1890-1930 By Sally Kohlstedt, Meena M. Balgopal Dec 2011

Review Of Teaching Children Science: Hands-On Nature Study In North America, 1890-1930 By Sally Kohlstedt, Meena M. Balgopal

The Prairie Naturalist

Many scientists and educators agree that the goal of science education is to prepare students "to know, use and interpret scientific explanations of the natural world," as cited in the National Research Council publication, Taking Science to School: Learning and Teaching Science in Grades K-8 (DuschI et al. 2007). Yet, many science instructors of K-12 and post-secondary students often rely on teacher-telling modes of pedagogy and neglect to engage their students in natural inquiry and scientific study that model the research methods used by scientists. As a result, many young people are not aware of how scientists make discoveries about …


Depth And Littoral Habitat Association Of Age-0 Yellow Perch In Two South Dakota Glacial Lakes, Daniel J. Dembkowski, Melissa R. Wuellner, David W. Willis Dec 2011

Depth And Littoral Habitat Association Of Age-0 Yellow Perch In Two South Dakota Glacial Lakes, Daniel J. Dembkowski, Melissa R. Wuellner, David W. Willis

The Prairie Naturalist

Yellow perch (Perca flavescens) are a recreationally important species and represent a key ecological component of glacial lake littoral fish assemblages (Stone 1996, Blackwell et al. 1999). Research has shown a generalized pattern of juvenile (age-0) yellow perch spatial distribution wherein larvae hatch in near-shore areas, migrate to limnetic areas where they remain for approximately 40 d, and then return to demersal behaviors and within near-shore littoral habitats (Noble 1975, Whiteside et al. 1985). However, anomalous distribution and habitat use by age-0 yellow perch has been observed in South Dakota glacial lakes (Fisher and Willis 1997) and the …


Massasauga Repatriation On A Restored Wet Prairie, Francis E. Durbian, Brian N. Lomas, Jeff Briggler, Paul Mckenzie, Tom Nagel Dec 2011

Massasauga Repatriation On A Restored Wet Prairie, Francis E. Durbian, Brian N. Lomas, Jeff Briggler, Paul Mckenzie, Tom Nagel

The Prairie Naturalist

The massasauga (Sistrurus catenatus) is a small rattlesnake that occurs from Texas to New York (Schmidt and Davis 1941, Conant and Collins 1991, Szymanski 1998). In Missouri, the Eastern massasauga rattlesnake (EMR; S. c. catenatus), a subspecies of the massasauga, occurs north and east of the Missouri River, is a former candidate for listing under the United States Endangered Species Act (Code of Federal Regulations 64 FR 57534; Szymanski 1998), and is listed as a state endangered species (Missouri Natural Heritage Program 2011). Missouri currently harbors five extant EMR populations (Johnson 2000, Durbian et al., unpublished report, …


Book Review Of Encyclopedia Of Biological Invasions, Edited By Daniel Simberloff And Marcel Rejmanek, Qinfeng Guo Dec 2011

Book Review Of Encyclopedia Of Biological Invasions, Edited By Daniel Simberloff And Marcel Rejmanek, Qinfeng Guo

The Prairie Naturalist

Book review of Encyclopedia of Biological Invasions, edited by Daniel Simberloff and Marcel Rejmanek.

Species introductions and consequent biotic invasions and homogenization are major components of global change that are drawing increasing concern and various levels of actions and reactions around the world. Invasion ecology has advanced rapidly during the last few decades, and the discipline is now increasingly integrated with the social and economic sciences. A better understanding of the invasion process and its effects is thus clearly needed. For basic research, invasion biology offers fascinating and sometimes unique opportunities for testing certain ecological or evolutionary theories and …


Modeling Parental Provisioning By Red-Winged Blackbirds In North Dakota, George M. Linz, Richard S. Sawin, Mark W. Lutman, William J. Bleier Dec 2011

Modeling Parental Provisioning By Red-Winged Blackbirds In North Dakota, George M. Linz, Richard S. Sawin, Mark W. Lutman, William J. Bleier

The Prairie Naturalist

Male red-winged blackbirds (Agelaius phoeniceus) exhibit a difference in nest provisioning rates along an east-west gradient in North America. North Dakota is located in the center of North America and harbors a large population of breeding red-winged blackbirds (RWBL). This location provided an opportunity to compare provisioning rates in the central U.S. with those reported for the eastern and western populations. We placed video cameras at RWBL nests to record male and female feeding trips. Thirty-four nests were located on territories with original males and 30 were on territories where a replacement (floater) male had taken over a …


Manuscript Content: Where Does It Belong?, Christopher N. Jacques Dec 2011

Manuscript Content: Where Does It Belong?, Christopher N. Jacques

The Prairie Naturalist

One of the most common issues we address during content editing of papers for The Prairie Naturalist (journal) is the appropriate placement of content in the text body. Based on my experience with the editorial process, content placement also is one of the issues that authors are most resistant to suggestions or do not fully understand why we are so persistent about it (Thompson 2010). One of the primary objectives of scientific writing is to concisely and accurately disseminate information. Scientific papers are structured to help both the author and reader accomplish this objective. The material that belongs in each …


Fall Field Report, August–November 2011, W. Ross Silcock Dec 2011

Fall Field Report, August–November 2011, W. Ross Silcock

Nebraska Bird Review

Overall, this fall migration was routine (waterfowl, warblers, sparrows), but there were a few unexpected highlights. The flooded Missouri River Valley provided habitat that led to some amazing fall counts: 3000 pelicans, 5700 coots, 338 American Golden-Plovers, and 1200 Pectoral Sandpipers. The 10,000 cormorants at Harlan County Reservoir may not have been affected by the Missouri River flooding, and were a record count also. While we're talking numbers, the martin roost in Omaha topped out at 50,000–55,000, and seasonal totals of 4 Red-necked Grebes and 15 Philadelphia Vireos for the state, 34 Mississippi Kites over Ogallala (it's often hard to …


Nebraska Bird Review (December 2011) 79(4), Whole Issue Dec 2011

Nebraska Bird Review (December 2011) 79(4), Whole Issue

Nebraska Bird Review

Fall Field Report, August–November 2011 ... 118

First Documented Nebraska Sighting of Brown-headed Nuthatch (Sitta pusilla) ... 136

First Discovery of a Four-Egg Clutch for Mountain Plover (Charadrius montanus) in Nebraska ... 139

Smith's Longspur in Knox County, Nebraska ... 141

Fall 2011 Bird Banding at Wildcat Hills Nature Center ... 142

Kearney Fall Field Days ... 143

Index to Volume 79 ... 148

Subscription and Organization Information ... 159


First Documented Nebraska Sighting Of Brown-Headed Nuthatch (Sitta Pusilla), Larry Einemann Dec 2011

First Documented Nebraska Sighting Of Brown-Headed Nuthatch (Sitta Pusilla), Larry Einemann

Nebraska Bird Review

Monday, November 8, 2010, was an unusually warm (upper 70s) autumn day for Lincoln, Nebraska, with no clouds and a wind from the southwest. I went to Holmes Lake Park in southeast Lincoln to scan for late migrating waterfowl and then to check the stand of conifers for the arrival of winter migrants. Around 11 A.M. I was attracted to a feeding group of six Black-capped Chickadees and three Red-breasted Nuthatches in the 30- to 40-year-old pines and Douglas firs north of Hyde Observatory. I noticed a differently patterned nuthatch in the group. I had seen a Pygmy Nuthatch several …


Kearney Fall Field Days Dec 2011

Kearney Fall Field Days

Nebraska Bird Review

The 2011 NOU Fall Field Days were held at the Iain Nicolson Center at the Audubon Rowe Sanctuary southeast of Kearney on September 23–25, and 55 members and friends were in attendance. The meeting was organized by Kent Skaggs.

Field trip destinations included Harlan County Reservoir, led by Kent Skaggs; the Rainwater Basin, led by Paul Dunbar; Bittern's Call WPA and Johnson Lake, led by John Murphy; and Sherman Reservoir, led by Robin Harding and Lanny Randolph. A final tally of 143 species was recorded Highlights included the Neotropic Cormorants at Harlan County Reservoir and Sherman Reservoir and the numerous …


Biological Analysis Of Prebiotics In Various Processed Food Matrices, Kristina Elise Moore Dec 2011

Biological Analysis Of Prebiotics In Various Processed Food Matrices, Kristina Elise Moore

Department of Food Science and Technology: Dissertations, Theses, and Student Research

The addition of prebiotic to a variety of food products has become a more common occurrence in recent years. Although research on the stability of prebiotics has been conducted, knowledge of the stability of prebiotics in processed foods is limited. The purpose of this research was to determine the biological stability of five prebiotics in a variety of food matrices when processed under various conditions. A biological test, the prebiotic activity assay, was used to test for stability of six food products (muffin, cookie, granola bar, breakfast cereal, sports drink, and bread) containing five different prebiotics (fructooligosaccharides (FOS), inulin, galactooligosaccharides …


Identification And Characterization Of Putative Allergens In Pecan Species, Jelena Spiric Dec 2011

Identification And Characterization Of Putative Allergens In Pecan Species, Jelena Spiric

Department of Food Science and Technology: Dissertations, Theses, and Student Research

The identification and characterization of soluble pecan proteins was found to be qualitatively dependent on the chemical characteristics of the extraction buffer that also had a significant influence on the total protein content of an extract. The ionic strength of the extraction buffer had a greater impact on protein extractability than pH. A high salt phosphate buffer (0.01 M PBS, 1M NaCl, pH 7.2) was used as an optimal extraction solution that allowed remarkable detection of clinically relevant allergens.

The 2S albumin family of seed storage proteins has been shown to be one of the most stable allergens, thus potentially …


Obituary: Jerry Ronald Choate, 1943-2009, Elmer J. Finck, Hugh H. Genoways, Justin D. Hoffman, Carleton J. Phillips, Robert J. Baker Dec 2011

Obituary: Jerry Ronald Choate, 1943-2009, Elmer J. Finck, Hugh H. Genoways, Justin D. Hoffman, Carleton J. Phillips, Robert J. Baker

University of Nebraska State Museum: Mammalogy Papers

Jerry Ronald Choate (1943–2009) had just retired as Director of the Sternberg Museum of Natural History and Professor of Biological Sciences, Fort Hays State University, Hays, Kansas, at the time of his death. Jerry served the American Society of Mammalogists in numerous capacities, including Recording Secretary, First Vice President, and most notably as a member and chair of the Board of Trustees.

The hallmark of Jerry’s life was to turn the ordinary into something magnificent. Whether it was his photography that changed an ordinary landscape into a magnificent masterpiece, or his convincing a reluctant graduate student that they could do …


The Feathers Of Winter, Paul A. Johnsgard Dec 2011

The Feathers Of Winter, Paul A. Johnsgard

Paul Johnsgard Collection

For many Nebraska birders, the last big event of the year is the Audubon Christmas Bird Count, which is held annually during the last week of December. It is an occasion to join with friends in a day out to try see as many species as possible in a single day. More importantly, it provides a database that, combined with those of more than 50,000 other observers, provides a highly documented population sample of early-winter birds throughout North America, Latin America and the Caribbean region. The tradition began in 1900, and as of 2011 there have been 111 national counts. …