Life Sciences Commons^™

Drosophila Vitelline Membrane Assembly: A Critical Role For An Evolutionarily Conserved Cysteine In The “Vm Domain” Of Sv23, T. Wu, Anita L. Manogaran, J. M. Beauchamp, Gail L. Waring

Biological Sciences Faculty Research and Publications

The vitelline membrane (VM), the oocyte proximal layer of the Drosophila eggshell, contains four major proteins (VMPs) that possess a highly conserved “VM domain” which includes three precisely spaced, evolutionarily conserved, cysteines (CX⁷CX⁸C). Focusing on sV23, this study showed that the three cysteines are not functionally equivalent. While substitution mutations at the first (C123S) or third (C140S) cysteines were tolerated, females with a substitution at the second position (C131S) were sterile. Fractionation studies showed that sV23 incorporates into a large disulfide linked network well after its secretion ceases, suggesting that post-depositional mechanisms are in place to …

11/13/2010 Swosu Vs Acu (Wide Angle), Southwestern Oklahoma State University

Football Videos

Football video of SWOSU vs ACU (Abilene Christian University) on November 13, 2010. Footage was compiled from smaller clips and has no audio.

Also attached is a PDF of the statistics from that game, collected from: https://static.swosuathletics.com/custompages/stats/football/foot2010/10-11-13.htm

Moving Towards A Complete Molecular Framework Of The Nematoda: A Focus On The Enoplida And Early-Branching Clades, Holly M. Bik, P. John D. Lambshead, W. Kelley Thomas, David H. Lunt

Hubbard Center for Genome Studies (HCGS)

Background: The subclass Enoplia (Phylum Nematoda) is purported to be the earliest branching clade amongst all nematode taxa, yet the deep phylogeny of this important lineage remains elusive. Free-living marine species within the order Enoplida play prominent roles in marine ecosystems, but previous Molecular phylogenies have provided only the briefest evolutionary insights; this study aimed to firmly resolve internal relationships within the hyper diverse but poorly understood Enoplida. In addition, we revisited the Molecular framework of the Nematoda using a rigorous phylogenetic approach in order to investigate patterns of early splits amongst the oldest lineages (Dorylaimia and Enoplia). Results: Morphological …

Meg And Fmri Fusion For Non-Linear Estimation Of Neural And Bold Signal Changes, Sergey M. Plis, Vince D. Calhoun, Michael Patrick Weisend, Tom Eichele, Terran D.R. Lane

Wright State Research Institute Publications

The combined analysis of magnetoencephalography (MEG)/electroencephalography and functional magnetic resonance imaging (fMRI) measurements can lead to improvement in the description of the dynamical and spatial properties of brain activity. In this paper we empirically demonstrate this improvement using simulated and recorded task related MEG and fMRI activity. Neural activity estimates were derived using a dynamic Bayesian network with continuous real valued parameters by means of a sequential Monte Carlo technique. In synthetic data, we show that MEG and fMRI fusion improves estimation of the indirectly observed neural activity and smooths tracking of the blood oxygenation level dependent (BOLD) response. In …

Accelerated Shelf Life Determination Of Antioxidant Stabilized High Oleic Sunflower And Canola Oils In Plastic Bottles, Christine Nicole Shearer

Theses and Dissertations

Vegetable oil is an important commodity; however, the majority of commercially available vegetable oils have a fairly short shelf life, which limits their usefulness for long term food storage, disaster relief, space travel, food aid programs, and military rations. Vegetable oils with high oleic acid and reduced linolenic acid contents, especially with added antioxidant combinations, were previously found to have significantly longer oil stability index (OSI) values than traditional vegetable oils. This study used accelerated shelf life testing to estimate the ambient shelf life of high oleic sunflower oil (HOSUN) and high oleic canola oil (HOCAN), each containing 1,000 ppm …

Leishmania Major Survival In Selective Phlebotomus Papatasi Sand Fly Vector Requires A Specific Scg-Encoded Lipophosphoglycan Galactosylation Pattern, Deborah E. Dobson, Shaden Kamhawi, Phillip Lawyer, Salvatore J. Turco, Stephen M. Beverley, David L. Sacks

Molecular and Cellular Biochemistry Faculty Publications

Phlebotomine sand flies that transmit the protozoan parasite Leishmania differ greatly in their ability to support different parasite species or strains in the laboratory: while some show considerable selectivity, others are more permissive. In "selective" sand flies, Leishmania binding and survival in the fly midgut typically depends upon the abundant promastigote surface adhesin lipophosphoglycan (LPG), which exhibits species- and strain-specific modifications of the dominant phosphoglycan (PG) repeat units. For the "selective" fly Phlebotomus papatasi PpapJ, side chain galactosyl-modifications (scGal) of PG repeats play key roles in parasite binding. We probed the specificity and properties of this scGal-LPG PAMP (Pathogen Associated …

Smokejumper Obituary: Ruska, Mitchell (Mccall 1963), National Smokejumper Association

Smokejumper Obituaries

No abstract provided.

Resonance Assignments And Secondary Structure Predictions Of The As(Iii) Metallochaperone Arsd In Solution, Jun Ye, Yanan He, Jack Skalicky, Barry P. Rosen, Timothy L. Stemmler

Biochemistry and Molecular Biology Faculty Publications

ArsD is a metallochaperone that delivers As(III) to the ArsA ATPase, the catalytic subunit of the ArsAB pump encoded by the arsRDABC operon of Escherichia coli plasmid R773. Conserved ArsD cysteine residues (Cys12, Cys13 and Cys18) construct the As(III) binding site of the protein, however a global structural understanding of this arsenic binding remains unclear. We have obtained NMR assignments for ArsD as a starting point for probing structural changes on the protein that occur in response to metalloid binding and upon formation of a complex with ArsA. The predicted solution structure of ArsD is in agreement with recently published …

Investigating The Spectrum Of Biological Activity Of Ring-Substituted Salicylanilides And Carbamoylphenylcarbamates, Jiahui Guo, Aidan Coffey, Et. Al.

Department of Biological Sciences Publications

In this study, a series of twelve ring-substituted salicylanilides and carbamoylphenylcarbamates were prepared and characterized. The compounds were analyzed using RP-HPLC to determine lipophilicity. They were tested for their activity related to the inhibition of photosynthetic electron transport (PET) in spinach (Spinacia oleracea L.) chloroplasts. Moreover, their site of action in the photosynthetic apparatus was determined. Primary in vitro screening of the synthesized compounds was also performed against mycobacterial, bacterial and fungal strains. Several compounds showed biological activity comparable with or higher than the standards 3-(3,4-dichlorophenyl)-1,1-dimethylurea, isoniazid, penicillin G, ciprofloxacin or fluconazole. The most active compounds showed minimal anti-proliferative activity …

Talk By Dr. A.L. Bement, Jr. On Energy Sustainability, Arden L. Bement Jr.

PPRI Digital Library

The following remarks were invited by Purdue's Energy Club based on questions prepared by the club students. The meeting took place on November 9, 2010 and was well attended. The responses to the questions elicited were many well posed follow-up questions and comments.

Land Use As It Relates To Land Slope, James D. Plourde, Bryan C. Pijanowski

GIS Day

The goal of this project is to analyze the relationship between the slope of land and agricultural land use. The output was analyzed to determine a threshold point at which agriculture practices drop off in relation to higher slope values. Slope of the land is important to agricultural practices because it impacts drainage and net crop primary production. Agricultural land was extracted from the 2001 National Land Cover Dataset using reclassification tools in ArcGIS 9.3. The slope of the contiguous United States was derived from the United States Geological Survey National Elevation Dataset by using the slope tool in the …

Sound Diversity In The Landscape: The Effects Of Land Use, Luis J. Villanueva-Rivera, Bryan C. Pijanowski

GIS Day

Climate change, land use change and the introduction of exotic species are the three most important anthropogenic threats to the ecosystems and their biodiversity. In order to evaluate the impacts these threats are having, ecologists need better methods to measure, in space and time, the biodiversity in a fast and scalable way. Our group is proposing the use of the sounds produced by animals in a landscape, the biophony, as a proxy for the biodiversity. As a first step in the quantification of biophony, we collected sound recordings from seven sites in the Tippecanoe County, Indiana. These sites were located …

Micrornas Reveal The Interrelationships Of Hagfish, Lampreys, And Gnathostomes And The Nature Of The Ancestral Vertebrate, Alysha M. Heimberg, Richard Cowper-Sal{Middle Dot}Lari, Marie Semon, Philip C. J. Donoghue, Kevin J. Peterson

Dartmouth Scholarship

Hagfish and lampreys are the only living representatives of the jawless vertebrates (agnathans), and compared with jawed vertebrates (gnathostomes), they provide insight into the embryology, genomics, and body plan of the ancestral vertebrate. However, this insight has been obscured by controversy over their interrelationships. Morphological cladistic analyses have identified lampreys and gnathostomes as closest relatives, whereas molecular phylogenetic studies recover a monophyletic Cyclostomata (hagfish and lampreys as closest relatives). Here, we show through deep sequencing of small RNA libraries, coupled with genomic surveys, that Cyclostomata is monophyletic: hagfish and lampreys share 4 unique microRNA families, 15 unique paralogues of more …

Diatoms As Indicators Of Water-Level Change In Freshwater Lakes, Julie A. Wolin, Jeffrey R. Stone

Biological, Geological, and Environmental Faculty Publications

Water-level changes result from a variety of geological, biological, and/or climatic processes. Many of these changes occur over long periods; others may be rapid or result from catastrophic events. In aquatic environments, diatoms are highly sensitive indicator organisms and their microfossils, deposited in lake sediments, can be used to infer environmental changes (Smol, 2008). Unambiguous diatom signals can be reconstructed from lakes isolated from marine or brackish waters (e.g. Fritz et al., this volume; Horton & Sawai, this volume). However, in freshwater systems lake-level changes are often recorded as increases in planktonic (free-floating) diatoms – although as discussed below, interpretation …

Colorful Fruit And Foliage, Larry A. Sagers

Archived Gardening Publications

No abstract provided.

Creating Fall Color, Larry A. Sagers

Archived Gardening Publications

No abstract provided.

Qualitative Study To Determine Quality Of Life Factors Based On Reported Efnep Success Stories, Megan L. Hlavacek

Department of Nutrition and Health Sciences: Dissertations, Theses, and Student Research

The purpose of this study was to develop an understanding of how adult participants describe quality of life during or after participation in the Expanded Food and Nutrition Education Program (EFNEP) at sites across the United States. The study analyzed success stories submitted by EFNEP staff and participants from sites in nine states from the years 2004 to 2010. A total of 1,057 success stories were analyzed.

The success stories were descriptions of the participants’ experiences with EFNEP. The stories were analyzed by highlighting words, phrases, and/or sentences, and creating a short one to three word codes that would best …

Flexible Bootstrapping-Based Ontology Alignment, Prateek Jain, Pascal Hitzler, Amit P. Sheth

Kno.e.sis Publications

BLOOMS (Jain et al, ISWC2010) is an ontology alignment system which, in its core, utilizes the Wikipedia category hierarchy for establishing alignments. In this paper, we present a Plug-and-Play extension to BLOOMS, which allows to flexibly replace or complement the use of Wikipedia by other online or offline resources, including domain-specific ontologies or taxonomies. By making use of automated translation services and of Wikipedia in languages other than English, it makes it possible to apply BLOOMS to alignment tasks where the input ontologies are written in different languages.

Dianna Krebs, Dianna Krebs, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Dianna Krebs has had an interesting path to school nutrition. After majoring in finance in college she worked as a bookkeeper, first at a bank, and then as the bookkeeper for her local school district, eventually becoming the bookkeeper for the child nutrition programs, where she rose through the ranks, becoming a coordinator, an assistant to the director, and finally the school nutrition director, where she currently supervises eighteen schools.

Lark Christian, Lark Christian, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Lark Christian has worked as a child care supervisor and statewide coordinator for child nutrition programs for the State of Mississippi. She is now the food service director for Jackson County, Mississippi.

11/06/2010 Swosu Vs Uco (Endzone Angle), Southwestern Oklahoma State University

Football Videos

Football video of the SWOSU vs UCO game on Nov. 6, 2010 in Weatherford, OK. Footage was compiled from individual clips and has no audio.

Also attached is a PDF of the statistics from the game, collected from: https://static.swosuathletics.com/custompages/stats/football/foot2010/10-11-06.htm

11/06/2010 Swosu Vs Uco (Sideline Angle), Southwestern Oklahoma State University

Football Videos

Football video of the SWOSU vs UCO game on Nov. 6, 2010 in Weatherford, OK. Footage was compiled from individual clips and has no audio.

Also attached is a PDF of the statistics from the game, collected from: https://static.swosuathletics.com/custompages/stats/football/foot2010/10-11-06.htm

Saundra Isabelle, Saundra Isabelle, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

Saundra Isabelle started working part-time in child nutrition when her children started school, but immediately fell in love with it. She quickly rose through the ranks and became a manager at Pass Christian Elementary, Middle, and High Schools for five years. She is now the food service director for the Pass Christian School District.

Judy Mumphrey, Judy Mumphrey, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

After earning bachelors and master’s degrees at the University of Southern Mississippi, Judy Mumphrey taught home economics for eleven and a half years, before moving into school food service. She started out as the School Foodservice Administrator at Pass Christian before moving to Bay-Waveland Schools, where she finished her career. Her biggest challenge over that career was dealing with the aftermath of Hurricane Katrina.

Wanda Salley, Wanda Salley, Institute Of Child Nutrition. Child Nutrition Archives.

Oral History Project (all interviews)

An Alabama native, Wanda Salley holds degrees from Auburn University and Mississippi State University, and is also a Registered Dietitian. After beginning her career in hospitals she transitioned into school food service, first becoming a food service director in Houston, MS, followed by stints in Greenville, Pass Christian, and Harrison County, MS schools.

Making Of Viral Replication Organelles By Remodeling Interior Membranes, Zsuzsanna Sasvari, Peter D. Nagy

Plant Pathology Faculty Publications

Positive-stranded RNA (+RNA) viruses exploit host cell machinery by subverting host proteins and membranes and altering cellular pathways during infection. To achieve robust replication, some +RNA viruses, such as poliovirus (PV), build special intracellular compartments, called viral replication organelles. A recent work from the Altan-Bonnett laboratory [1] gave new insights into the formation of poliovirus replication organelles, which are unique subcellular structures containing many individual replication complexes as a result of dynamic cellular membrane remodeling.

Aminopeptidases Do Not Directly Degrade Tau Protein, K. Martin Chow, Hanjun Guan, Louis B. Hersh

Molecular and Cellular Biochemistry Faculty Publications

BACKGROUND: Tau hyperphosphorylation and aggregation to form intracellular neurofibrillar tangles is prevalent in a number of tauopathies. Thus there is current interest in the mechanisms involved in Tau clearance. It was recently reported that Tau can be degraded by an aminopeptidase known as the puromycin sensitive aminopeptidase (PSA). Until now PSA has been reported to only cleave peptides, with the largest reported substrates having 30-50 amino acids. We have studied this unique PSA cleavage reaction using a number of different PSA preparations.

RESULTS: An N-terminally His tagged-PSA was expressed and purified from Sf9 insect cells. Although this PSA preparation cleaved …

Translation Elongation Factor 1a Facilitates The Assembly Of The Tombusvirus Replicase And Stimulates Minus-Strand Synthesis, Zhenghe Li, Judit Pogany, Steven Tupman, Anthony M. Esposito, Terri Goss Kinzy, Peter D. Nagy

Plant Pathology Faculty Publications

Replication of plus-strand RNA viruses depends on host factors that are recruited into viral replicase complexes. Previous studies showed that eukaryotic translation elongation factor (eEF1A) is one of the resident host proteins in the highly purified tombusvirus replicase complex. Using a random library of eEF1A mutants, we identified one mutant that decreased and three mutants that increased Tomato bushy stunt virus (TBSV) replication in a yeast model host. Additional in vitro assays with whole cell extracts prepared from yeast strains expressing the eEF1A mutants demonstrated several functions for eEF1A in TBSV replication: facilitating the recruitment of the viral RNA template …

Kinematic Changes Of The Tennis Forehand Ground Stroke As Post Contact Ball Speed Increases, Merrill D. Funk

Theses and Dissertations

Neuromuscular and kinematic patterns during the tennis forehand have been studied extensively. However, no one has evaluated potential upper-extremity kinematic changes during the forehand as ball speed increases. The purpose of this study was to evaluate changes in shoulder and trunk kinematics as forehand ball speed increased, in an attempt to better understand how kinematics may promote forehand ball speed. Peak trunk rotation angle, shoulder horizontal abduction/adduction and internal/external rotation angle, and corresponding angular velocities were measured between initial backswing and ball contact during forehands that were performed at three different speeds (50%, 75%, and 100% of maximal post-impact ball …

The Mycobacterium Tuberculosis Drugome And Its Polypharmacological Implications, Sarah L. Kinnings, Li Xie, Kingston H. Fung, Richard M. Jackson, Lei Xie, Phillip E. Bourne

Publications and Research

We report a computational approach that integrates structural bioinformatics, molecular modelling and systems biology to construct a drug-target network on a structural proteome-wide scale. The approach has been applied to the genome of Mycobacterium tuberculosis (M.tb), the causative agent of one of today’s most widely spread infectious diseases. The resulting drug-target interaction network for all structurally characterized approved drugs bound to putative M.tb receptors, we refer to as the ‘TB-drugome’. The TB-drugome reveals that approximately one-third of the drugs examined have the potential to be repositioned to treat tuberculosis and that many currently unexploited M.tb receptors may be chemically druggable …