Life Sciences Commons^™

Land Cover Inventory Of The Niobrara River Watershed, Patti R. Dappen, James W. Merchant

Nebraska Forest Service: Publications

The Niobrara River watershed covers a large area in northern Nebraska, and neighboring parts of South Dakota and Wyoming, and is an important component of statewide natural resources management. Under ongoing agreements with the U.S. Bureau of Reclamation (BoR), the Nebraska Game and Parks Commission are charged with the development of management plans for various BoR projects in the Niobrara River watershed in Nebraska. A detailed delineation of land use and land cover patterns was needed in order to define and implement effective management strategies.

Nebraska Forest Services: Woody Biomass Energy: Nebraska’S Untapped Resource, Dr. Scott Josiah, Dennis Adams

Nebraska Forest Service: Publications

Energy security is a vital issue in the United States. Heavy dependence on fossil fuels obtained from politically volatile areas, an emerging consensus that carbon emissions must be substantially reduced, and dramatically increasing costs of fuel oil and natural gas are driving the urgent need for alternative energy sources, both in Nebraska and nationally.

Wood Energy Can Help Solve Pressing Problems In Nebraska By; Nebraska’s Available Woody Biomass Resources; Woody Biomass—A Proven, Reliable Energy Source For Nebraska And Beyond; Completed Engineering Feasibility Studies For Conversion To Woody Biomass Energy; Additional Facilities Interested In Or Currently Conducting Feasibility Studies; Other Emerging …

Nebraska’S Unique Forest Resources, Nebraska Forest Services, Dr. Scott J. Josiah

Nebraska Forest Service: Publications

Many Nebraskans would be surprised to learn that in our largely agricultural and grassland state, unique and diverse forest resources are substantial and growing; Nebraska’s Riparian Forest Resources; Nebraska’s Ponderosa Pine Forest Resources; Nebraska’s Niobrara Valley Forest Resources

Nebraska Forestry Consultants, Dennis Adams

Nebraska Forest Service: Publications

This directory includes individuals and firms that offer forestry consulting services in Nebraska. It was originally developed from a “Nebraska Forestry Consultants Survey” conducted in April, 2002. Corrections/additions are periodically incorporated. Endorsement by the Nebraska Forest Service is not intended or inferred. Corrections or additions may be submitted to the Nebraska Forest Service, C/O Dennis Adams, 203C Forestry Hall, UNL East Campus, Lincoln, NE 68583-0815. Phone (402) 472-5822, E-mail: [email protected]

Coaching Efficacy With Academic Leaders: A Phenomenological Investigation, Deanna Lee Vansickel-Peterson

College of Education and Human Sciences: Dissertations, Theses, and Student Research

The purpose of this psychological phenomenological research was to understand the efficacy of life coaching from the perspective of academic leaders. To date, not one investigation or attempt has been made towards the above stated purpose. This study includes a theoretical overview and a review of the coaching literature from Socrates (469-399 BC) to current day Humanistic theory presented in part by Roger (1902-1987). This process included data collection from five academic leaders who have been coached for at least two years. Levels of analysis of 365 statements, quote and/or comments produced finding of efficacy in life coaching with academic …

Fusarium Head Blight: Winter Wheat Cultivar Responses And Characterization Of Pathogen Isolates, John Fredy Hernandez Nopsa

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Fusarium Head Blight (FHB) is a destructive disease of wheat (Triticum aestivum L). FHB reduces yield and grain quality and causes accumulation of the mycotoxin deoxynivalenol (DON) in grain. Cultivar resistance is one of the most effective management strategies for FHB. Experiments were conducted to 1) identify winter wheat cultivars with resistance to FHB and DON accumulation, 2) determine the effect of winter wheat cultivar on the relationship between FHB and DON concentration, and 3) identify the major species of Fusarium causing FHB in Nebraska and characterize its isolates. Differences (P ≤ 0.05) were detected among cultivars in …

An Archaeal Trna-Synthetase Complex That Enhances Aminoacylation Under Extreme Conditions, Vlatka Godinic-Mikulcic, Jelena Jaric, Corinne D. Hausmann, Michael Ibba, Ivana Weygand-Durasevic

Biology, Chemistry, and Environmental Sciences Faculty Articles and Research

Aminoacyl-tRNA synthetases (aaRSs) play an integral role in protein synthesis, functioning to attach the correct amino acid with its cognate tRNA molecule. AaRSs are known to associate into higher-order multi-aminoacyl-tRNA synthetase complexes (MSC) involved in archaeal and eukaryotic translation, although the precise biological role remains largely unknown. To gain further insights into archaeal MSCs, possible protein-protein interactions with the atypical Methanothermobacter thermautotrophicus seryl-tRNA synthetase (MtSerRS) were investigated. Yeast two-hybrid analysis revealed arginyl-tRNA synthetase (MtArgRS) as an interacting partner of MtSerRS. Surface plasmon resonance confirmed stable complex formation, with a dissociation constant (KD) of 250 nm. Formation of the MtSerRS·MtArgRS complex …

A New Species Of Pristimantis (Anura: Strabomantidae) From The Amazonian Lowlands Of Northern Peru, Edgar Lehr, Luis Gagliardi Urrutia

Scholarship

We describe a new species of Pristimantis from the vicinity of Puerto Almendras, ca. 17 km straight southwest of Iquitos, Provincia Maynas, Region Loreto, Peru. With seven other species (P. carvalhoi, P. croceoinguinis, P. divnae, P. lirellus, P. minutulus, P. toftae, P. variabilis) from the Amazonian lowlands and lower eastern Andes in Peru, the new species shares a yellow groin and broadly expanded discs on fingers and toes. However, the new species can be distinguished by the follow¬ing combination of characters: snout–vent length 14.8 mm in the single male, 20.0–22.0 mm in females, strongly tubercular dorsal skin, absence of dorsolateral …

Role Of Alpha 7 Nicotinic Acetylcholine Receptor In Calcium Signaling Induced By Prion Protein Interaction With Stress-Inducible Protein 1, Flavio H. Beraldo, Camila P. Arantes, Tiago G. Santos, Nicolle G. T. Queiroz, Kirk Young, Jane R. Rylett, Regina P. Markus, Marco A. M. Prado, Vilma R. Martins

Anatomy and Cell Biology Publications

The prion protein (PrP(C)) is a conserved glycosylphosphatidyl-inositol-anchored cell surface protein expressed by neurons and other cells. Stress-inducible protein 1 (STI1) binds PrP(C) extracellularly, and this activated signaling complex promotes neuronal differentiation and neuroprotection via the extracellular signal-regulated kinase 1 and 2 (ERK1/2) and cAMP-dependent protein kinase 1 (PKA) pathways. However, the mechanism by which the PrPC-STI1 interaction transduces extracellular signals to the intracellular environment is unknown. We found that in hippocampal neurons, STI1-PrP(C) engagement induces an increase in intracellular Ca(2+) levels. This effect was not detected in PrP(C)-null neurons or wild-type neurons treated with an STI1 mutant unable to …

Proteoliposome Proton Flux Assays Establish Net Conductance, Ph-Sensitivity, And Functional Integrity Of A Novel Truncate Of The M2 Ion "Channel" Of Influenza A, Emily Peterson

Theses and Dissertations

A novel truncate of Influenza A M2 protein (residues 22-62), incorporated into a uniquely tailored proteoliposome proton uptake assay, demonstrated proton flux more characteristic of an ion transporter than a traditional ion "channel." The liposome paradigm was essential for testing the conductance activity of this M2 truncate at a range of extraphysiological pHs appropriate for channel vs. transport function determination. In addition to transporter-typical proton flux, M2(22-62) showed the key characteristics of functional integrity: selective proton uptake into liposomes and block of uptake by amantadine. Two sets of proteoliposome proton flux assays were carried out, Set 1 at pH values …

Systematic Approach To Electrostatically Induced 2d Crystallization Of Nanoparticles At Liquid Interfaces, Sumit Kewalramani, Suntao Wang, Yuan Lin, Huong Giang Nguyen, Qian Wang, Masafumi Fukuto, Lin Yang

Faculty Publications

We report an experimental demonstration of a strategy for inducing two-dimensional (2D)crystallization of charged nanoparticles on oppositely charged fluid interfaces. This strategy aims to maximize the interfacial adsorption of nanoparticles, and hence their lateral packing density, by utilizing a combination of weakly charged particles and a high surface charge density on the planar interface. In order to test this approach, we investigated the assembly of cowpea mosaic virus (CPMV) on positively charged lipid monolayers at the aqueous solution surface, by means of in situX-ray scattering measurements at the liquid–vapor interface. Theassembly was studied as a function of the solution …

Antimicrobial And Anticancer Activity Of Essential Oils From Guatemalan Medicinal Plants, Andrew B. Miller

Theses and Dissertations

Guatemalan medicinal plants were collected and screened for the presence of essential oils using steam distillation. Oil was found in 63 species from 24 families and was tested in tube dilution assays for activity against Escherichia coli, Staphylococcus aureus, Streptococcus mutans, Lactobacillus acidophilus and Candida albicans. Several essential oils were highly active with 20 instances of oils inhibiting the microbes at an MIC of 0.31 µl/ml. Oils were also tested against cancerous and established cell lines using a 15% (v/v) agar-media which was developed to improve essential oil solubility. Assays were performed against three cancer lines: …

The Use Of Genetics For The Management Of A Recovering Population: Temporal Assessment Of Migratory Peregrine Falcons In North America, Jeff A. Johnson, Sandra L. Talbot, Kurt K. Burnham, George K. Sage, Joseph W. Brown, Tom L. Maechtle, William S. Seegar, Michael A. Yates, Bud Anderson, David P. Mindell

Raptor Research Center Publications and Presentations

Background: Our ability to monitor populations or species that were once threatened or endangered and in the process of recovery is enhanced by using genetic methods to assess overall population stability and size over time. This can be accomplished most directly by obtaining genetic measures from temporally-spaced samples that reflect the overall stability of the population as given by changes in genetic diversity levels (allelic richness and heterozygosity), degree of population differentiation (F_ST and D_EST), and effective population size (N_e). The primary goal of any recovery effort is to produce a longterm self-sustaining …

Muscle Receptor Organs In The Crayfish Abdomen: A Student Laboratory Exercise In Proprioception, Bonnie Leksrisawat, Ann S. Cooper, Allison B. Gilberts, Robin L. Cooper

Biology Faculty Publications

The primary purpose of this experiment is to demonstrate primary sensory neurons conveying information of joint movements and positions as proprioceptive information for an animal. An additional objective of this experiment is to learn anatomy of the preparation by staining, dissection and viewing of neurons and sensory structures under a dissecting microscope. This is performed by using basic neurophysiological equipment to record the electrical activity from a joint receptor organ and staining techniques. The muscle receptor organ (MRO) system in the crayfish is analogous to the intrafusal muscle spindle in mammals, which aids in serving as a comparative model that …

Characterization Of The Lkb1-Mo25-Strad Ampkk Complex In Adult Mouse Skeletal Muscle, Cody Don Smith

Theses and Dissertations

In liver tissue, the AMP-activated protein kinase kinase (AMPKK) complex was identified as the association of LKB1, MO25α/β, and STRADα/β proteins; however, this complex has yet to be characterized in skeletal muscle. In this report, we demonstrate the expression of the LKB1-MO25-STRAD AMPKK complex in adult skeletal muscle, confirm the absence of mRNA splice variants, and report the relative mRNA expression levels of these complex-forming proteins. To facilitate this characterization we used control (ctrl) and muscle-specific LKB1 knockout (LKB1-/-) mice. LKB1 detection in untreated ctrl and LKB1-/- muscle lysates revealed two protein bands at approximately 50 and 60 kDa; although, …

The Influence Of Persistent Pathogens On Circulating Levels Of Inflammatory Markers: A Cross-Sectional Analysis From The Multi-Ethnic Study Of Atherosclerosis, Aydin Nazmi, Ana V. Diez-Roux, Nancy S. Jenny, Michael Y. Tsai, Moyses Szklo, Allison E. Aiello

Food Science and Nutrition

Background: Systemic inflammation is linked to cardiovascular risk, but the influence of persistent pathogens, which are conventionally dichotomously categorized, on circulating levels of inflammatory markers is not clear. Antibody levels of pathogens have not been examined in relation to inflammation.
Methods: Using data from a subsample of the Multi-Ethnic Study of Atherosclerosis, we examined circulating levels of interleukin-6 (IL-6), C-reactive protein (CRP) and fibrinogen in relation to five common persistent pathogens: cytomegalovirus, herpes simplex virus-1, Hepatitis A virus, Helicobacter pylori and Chlamydia pneumoniae. We tested the hypothesis that the number of seropositive pathogens (based on conventional …

Terminology Enhanced Ehr: Integration Of Archetypes And Terminology, An Implementation Experience, Sheng Yu, Damon Berry

Reports

The integration of terminology and EHR information models is an important step in the journey towards semantic interoperability. Archetypes and two-level models for EHRs provide a mechanism that not only applies constraints on clinical content but also ensures effective terminology binding. However the lack of a standardised mechanism to bind terminology to the EHR and the difficulty of systematically coding clinical content, has led to a number of possible implementation choices. This study presents a review of the problems that may occur when working with modern terminology systems and discusses some related state of art technologies. The paper aims to …

Physiological Recordings Of High And Low Output Nmjs On The Crayfish Leg Extensor Muscle, Wenhui Wu, Robin L. Cooper

Biology Faculty Publications

We explain in detail how to expose and conduct electrophysiological recordings of synaptic responses for high (phasic) and low (tonic) output motor neurons innervating the extensor muscle in the walking leg of a crayfish. Distinct differences are present in the physiology and morphology of the phasic and tonic nerve terminals. The tonic axon contains many more mitochondria, enabling it to take a vital stain more intensely than the phasic axon. The tonic terminals have varicosities, and the phasic terminal is filiform. The tonic terminals are low in synaptic efficacy but show dramatic facilitated responses. In contrast, the phasic terminals are …

Effects Of Diabetic State And Gender On Pro-Inflammatory Cytokine Secretion By Human Macrophages Infected With Burkholderia Pseudomallei, Annette J. Blam

Theses and Dissertations

Burkholderia pseudomallei is a gram-negative opportunistic soil pathogen that causes the life-threatening disease melioidosis. It is endemic in Northern Australia and Southeast Asia but can be found throughout many other regions in the world. Diabetes mellitus is a predisposing risk factor for infection with this organism and it has been demonstrated that diabetic males are particularly susceptible to severe infection. Previous research suggested that monocytes isolated from the whole blood of diabetic males demonstrated a decreased ability to produce the proinflammatory cytokines IL-1β and IL-8. We hypothesized that monocyte-derived macrophages from diabetic males would also secrete lower levels of pro-inflammatory …

Work Towards The Isolation And Characterization Of The Muscle Isoform Of Glucose 1,6-Bisphosphatase, Caleb J. Hiller

Theses and Dissertations

Glucose 1,6-bisphosphate is an important small molecule involved in the regulation of glycolysis. Four enzymes synthesize this compound. One enzyme is known to degrade it, glucose 1,6-bisphosphatase. Other groups have produced work that indicates that there are two isoforms of this enzyme, one predominant in the brain and one in the muscle. This thesis contains the work performed in attempts to isolate and characterize the muscle isoform of glucose 1,6-bisphosphatase. While this enzyme was not isolated, much was learned about it and the results from this work may help in the future identification of this enzyme.

Network Analysis And Comparative Phylogenomics Of Micrornas And Their Respective Messenger Rna Targets Using Twelve Drosophila Species, M Ryan Woodcock

Theses and Dissertations

MicroRNAs represent a special class of small (~21–25 nucleotides) non-coding RNA molecules which exert powerful post-transcriptional control over gene expression in eukaryotes. Indeed microRNAs likely represent the most abundant class of regulators in animal gene regulatory networks. This study describes the recovery and network analyses of a suite of homologous microRNA targets recovered through two different prediction methods for whole gene regions across twelve Drosophila species. Phylogenetic criteria under an accepted tree topology were used as a reference frame to 1) make inference into microRNA-target predictions, 2) study mathematical properties of microRNA-gene regulatory networks, 3) and conduct novel phylogenetic analyses …

Commercializing The Hybrid Hazelnut As A Bioenergy And Food Crop For The Central States

Nebraska Forest Service: Publications

Initiative Goal; What Is The Hybrid Hazelnut?; Superior Hybrid Hazelnuts in Nebraska; Hybrid Hazelnuts Solve Economic, Energy and Environmental Problems By: Requirements for Large-Scale Commercialization of Hybrid Hazelnuts; Resources Required For Rapid Commercialization

Forestry Best Management Practices For Nebraska

Nebraska Forest Service: Publications

Introduction;Roads;Timber Harvesting;Tree Planting;Forest Improvement and Protection

Planning For Forest Stewardship: A Desk Guide, D. Ramsey Russell Jr., Susan Stein

Nebraska Forest Service: Publications

Since 1991, the U.S. Department of Agriculture (USDA) Forest Service Forest Stewardship Program has assisted over 200,000 landowners in preparing multipurpose management plans for areas encompassing more than 20 million acres of nonindustrial private forest (NIPF). These plans promote the long-term sustainability of private forests by balancing future public needs for forest products with the need for protecting and enhancing watershed productivity, air and water quality, fish and wildlife habitat, and threatened and endangered species.

Conducting A Prescribed Burn And Prescribed Burning Checklist, Jerry D. Volesky, James L. Stubbendieck, Rob B. Mitchell

Nebraska Forest Service: Publications

The interacting forces of climate, fire and grazing formed and maintained the grasslands of the Great Plains of North America. Native Americans were the first to use fire to manipulate plant communities primarily to attract bison to lush regrowth. With the arrival of Europeans, the deliberate setting of fire was greatly curtailed because of the loss of property and potential loss of life associated with wildfires. Today, land managers realize that fire or prescribed burning is an effective tool to manage grassland

Nebraska Community Forestry Awards Program

Nebraska Forest Service: Publications

Each year the Nebraska Forest Service recognizes outstanding programs and projects in Community Forestry. There are nine categories for nominations. Awards are presented each year at the annual Tree City USA Conference and Awards Program held in Lincoln. The NFS encourages nominations. Deadline for each year will be the end of January but submissions can be made at any time.

To nominate a program, citizen, group, or business please fill out the form on the second page. Nomination categories are listed on the se for your convenience.

The Landscape Similarity Toolbox: New Tools For Optimizing The Location Of Control Sites In Experimental Studies, Thomas E. Dilts, Jian Yang, Peter J. Weisberg

Articles

Large‐scale manipulative experiments are critically important for linking ecological theory with land management at a relevant spatial scale. Statistically powerful inferential approaches such as the before‐after‐control‐impact design involve pairing a small number of treatment sites with control sites of analogous ecological structure and landscape context. Pairing treatment and control sites that are as analogous as possible is an important step to ensuring that differences are due to a treatment effect. The Landscape Similarity Toolbox provides tools for optimizing the location of potential control sites based upon the spatial characteristics of the treatment site.

Differences In Hiv Burden And Immune Activation Within The Gut Of Hiv-Positive Patients Receiving Suppressive Antiretroviral Therapy, Steven A. Yukl, Sara Gianella, Elizabeth Sinclair, Lorrie Epling, Qingsheng Li, Lijie Duan, Alex L.M. Choi, Valerie Girling, Terence Ho, Peilin Li, Katsuya Fujimoto, Harry Lampiris, C. Bradley Hare, Mark Pandori, Ashley T. Haase, Huldrych F. Günthard, Marek Fischer, Amandeep K. Shergill, Kenneth Mcquaid, Diane V. Havlir, Joseph K. Wong

School of Biological Sciences: Faculty Publications

Background. The gut is a major reservoir for human immunodeficiency virus (HIV) in patients receiving antiretroviral therapy (ART). We hypothesized that distinct immune environments within the gut may support varying levels of HIV.

Methods. In 8 HIV-1-positive adults who were receiving ART and had CD4⁺ T cell counts of >200 cells/μL and plasma viral loads of <40 copies/mL, levels of HIV and T cell activation were measured in blood samples and endoscopic biopsy specimens from the duodenum, ileum, ascending colon, and rectum.

Results. HIV DNA and RNA levels per CD4⁺ T cell were higher in all 4 gut sites compared with those in the blood. HIV DNA levels increased from the duodenum to the rectum, whereas the median HIV …

Electromyographic Analysis Of The Infraspinatus And Deltoid Muscles During Shoulder External Rotation Exercises With And Without A Towel Roll, Kazuto Sakita

Theses and Dissertations

Standing and sidelying external rotation exercises produce high activation of the deltoid and infraspinatus. Holding a towel roll under the arm at 30° shoulder abduction during these exercises may decrease deltoid activity and increase infraspinatus activity. The objective was to determine if the addition of a towel under the arm during standing and sidelying external rotation affects EMG activity of the infraspinatus, middle and posterior deltoid, and pectoralis major, compared to the no towel condition. 20 male volunteers (age; 26 ± 3, height; 1.80 m ± .07 m, weight; 77 kg ± 10 kg) had right dominant hand, bilaterally healthy …

Caribbean Corals In Crisis: Record Thermal Stress, Bleaching, And Mortality In 2005, Erinn M. Muller, Mark C. Eakin, Jessica A. Morgan, Scott F. Heron, Tyler B. Smith, Gang Liu, Lorenzo Alvarez-Filip, Bart J. Baca, Erich Bartles, Carolina Bastidas

Ocean Engineering and Marine Sciences Faculty Publications

The rising temperature of the world's oceans has become a major threat to coral reefs globally as the severity and frequency of mass coral bleaching and mortality events increase. In 2005, high ocean temperatures in the tropical Atlantic and Caribbean resulted in the most severe bleaching event ever recorded in the basin. Methodology/Principal Findings: Satellite-based tools provided warnings for coral reef managers and scientists, guiding both the iming and location of researchers' field observations as anomalously warm conditions developed and spread across the greater Caribbean region from June to October 2005. Field surveys of bleaching and mortality exceeded prior efforts …